RDB15276 - mAG-zGeminin(1/100) / pT2KXIGdeltain

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

mAG-zGeminin(1/100) / pT2KXIGdeltain (#RDB15276)

zGem-based S/G2/M marker for zebrafish cells to label nuclei with green fluorescent protein mAG. A component of the zFucci.

Clone info.	zGem-based S/G2/M marker for zebrafish cells to label nuclei with green fluorescent protein mAG. A component of the zFucci.
Vector backbone	pT2KXIGdeltain (plasmid)
Size of vector backbone	8.1 kb
Selectable markers	Amp^r
Gene/insert name	zebrafish zGeminin cDNA Galaxeidae stony coral GFP cDNA
Reference of insert sequence	AB505860 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions:
The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. MTAに書く付加的使用条件： Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
備考	提供条件は英文のまま記載してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15276	mAG-zGeminin(1/100) / pT2KXIGdeltain	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The mAG-zGeminin(1/100) / pT2KXIGdeltain was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15276).

Reference section:

Sugiyama, M., Sakaue-Sawano, A., Iimura, T., Fukami, K., Kitaguchi, T., Kawakami, K., Okamoto, H., Higashijima, S., Miyawaki, A., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15276_A7H9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F2 () Sequence file: RDB15276_A7H9a.seq
>D04498I7_A7H9_2_EF1a-C_F2_A06_03_ABI24.ab1 1 GATTGGGGGT TAAGGCCCGG TTCGCTCTCT TCCTCACCGG GTCTGCGGCG AGTTCTAGCT 61 GAAGCTTCCT GCAGGTCGAC CGATCCTGAG AACTTCAGGG TGAGTTTGGG GACCCTTGAT 121 TGTTCTTTCT TTTTCGCTAT TGTAAAATTC ATGTTATATG GAGGGGGCAA AGTTTTCAGG 181 GTGTTGTTTA GAATGGGAAG ATGTCCCTTG TATCACCATG GACCCTCATG ATAATTTTGT 241 TTCTTTCACT TTCTACTCTG TTGACAACCA TTGTCTCCTC TTATTTTCTT TTCATTTTCT 301 GTAACTTTTT CGTTAAACTT TAGCTTGCAT TTGTAACGAA TTTTTAAATT CACTTTTGTT 361 TATTTGTCAG ATTGTAAGTA CTTTCTCTAA TCACTTTTTT TTCAAGGCAA TCAGGGTATA 421 TTATATTGTA CTTCAGCACA GTTTTAGAGA ACAATTGTTA TAATTAAATG ATAAGGTAGA 481 ATATTTCTGC ATATAAATTC TGGCTGGCGT GGAAATATTC TTATTGGTAG AAACAACTAC 541 ACCCTGGTCA TCATCCTGCC TTTCTCTTTA TGGTTACAAT GATATACACT GTTTGAGATG 601 AGGATAAAAT ACTCTGAGTC CAAACCGGGC CCCTCTGCTA ACCATGTTCA TGCCTTCTTC 661 TCTTTCCTAC AGCTCCTGGG CAACGTGCTG GTTGTTGTGC TGTCTCATCA TTTTGGCAAA 721 GAATTCCTCG ACGGATCCAC TAGTAACGGC CGCCAGTGTG CTGGAATTCG CCACCATGGT 781 GAGCGTGATC AAGCCCGAGA TGAAGATCAA GCTGTGCATG AGGGGCACCG TGAACGGCCA 841 CAACTTCGTG ATCGAGGGCG AGGGCAAGGG CAACCCCTAC GAGGGCACCC AGATCCTGGA 901 CCTGAACGTG ACCGAGGGCG CCCCCCCTGC CCTTCGCCTA CGACATCCTG ACCACCGTGT 961 TCCAGTACGG CAACAGGGCC TTCACCAAGT ACCCCGCCGA CATCCAGGGA CTACTTTCAA 1021 GCAGACCCTT CCCCGAGGGC TACCAACTGG AGAGGAGCAT GAACCTACGA GGAACAGGCC 1081 ATCTGCACGC CACCAGCACA TCAGCATGAG GGCGACCTGC CTTCCTCTAC GACAACCAGG 1141 GTTCGA //
Primer: pAxCALNL_F1 () Sequence file: RDB15276_A7H9b.seq
>D04498I7_A7H9_2_pAxCALNL_F1_B06_06_ABI24.ab1 1 CTCAATTAAA TCTTATCATG TCTGGATCAT CATCGATTAC TCATAGGCCT CTTGTGTGAC 61 CCCCTCAGGC TGGTTTTCAT TTTCTGCATT TGTGGATTTG ACAGCCACCT CTGCTTTGAC 121 TCTCTTTGAA CCCTTAACTT GCTCGGCGCT CCACATCTTC CTTTTGGCCA CTGCTTTACC 181 ATTCTCAATT ATACGACCAA GGTTTTTGTT TACAGCAGAG GGCTGAAGCA CCTGAAGCGT 241 CCTCCTCCCC ATTCCTGCTC CAGATGTTGG CGCTACCAGG AATTTCTTGA TGTTTTCAGA 301 CGGGTTTTCT GCATTCTTTG GTCTTCTGAT GGAACTCATC TCGAGCGGCC GCCAGTGTGA 361 TGGATATCCC TTGGCCTGGC TGGGCAGCAT GGAGTACCTG GCCACGGCGT TCTCGTACAG 421 CTTCACCTTG TTGTAGTCCT TGTCGTGCTT CAGGATCTCG ATCCTGTGGT CGATCTTGTG 481 GGCGTCGGGC AGCCTCACCT CCTTCTTGGC CTTGTAGGTG GTCTTGAAGT CGCACCTGTA 541 GTGGCCGCCG CCCTCCAGCA GCAGCCTCAT GTTCACGTCG CCCTTCAGCA CGCCGTCCTC 601 CACGTACATC TTCTCGGTGC TGGGCTCCCA CTTCAGGGTC TTCTTCTGCA TCACGGGGCC 661 GTTGGGGGGG AAGTTGGTGC CGTCGAACCT GATGTCGTAG AAGAAGCAGT CGCCCCTCAT 721 GCTGATGTTG CTGGTGGCGG TGCAGATGCC CTGGTCCTCG TAGGTCATGC TCCTCTCCCA 781 GTGGTAGCCC TCGGGGAAGG TCTGCTTGAA GTAGTCCTGG ATGTCGGCGG GGTACTTGGT 841 GAAGGCCCTG TTGCCGTACT GGAACACGGT GGTCAGGATG TCGTAGGCGA AGGGCAGGGG 901 GGCGCCCTCG GTCACGTTCA GGTCCAGGAT CTGGGTGCCC TCGTAGGGTT TGCCCTTGCC 961 CTCGCCCTCG ATCACGAAGT TGTGGCCGTT CACGGGTGCC CCTCATGCAC AGCTTGATCT 1021 TCATCTTCGG GCTTGATCAC GCTTCACCAA TGGGTGGGCG AAATTCCAGC ACACTTGGGC 1081 GGGCCCGTTA CCTAGTGATC CGGTCGGAAG GAAATTTTCT TTTTGACCAA AAATGTGAAT 1141 GAGAGAACCC AGCACAC //
Primer: EF1a-N_R2 () Sequence file: RDB15276_A7H9c.seq
>D04498I7_A7H9_2_EF1a-N_R2_C06_09_ABI24.ab1 1 TTACGGGCAA GAATTGCAAG TTTGATTTGC ATTGGAAAGG GTCGCTGGCT TTTGTGTTAC 61 ACGCCCCTTA TTTGTGCTTG ATTAGATGAT CCCCCTGCTC GAGGGCCCAT CTGGCCTGTG 121 TTTCAGACAC CAGGGAGTCT CTGCTCACGT TTCCTGCTAT TTGCAGCCTC TCTATCAAGA 181 CTAATACACC TCTTCCCGCA TCGGCTGCCT GTGAGAGGCT TTTCAGCACT GCAGGATTGC 241 TTTTCAGCCC CAAAAGAGCT AGGCTTGACA CTAACAATTT TGAGAATCAG CTTCTACTGA 301 AGTTAAATCT GAGGTTTTAC AACTTTGAGT AGCGTGTACT GGCATTAGAT TGTCTGTCTT 361 ATAGTTTGAT AATTAAATAC AAACAGTTCT AAAGCAGGAT AAAACCTTGT ATGCATTTCA 421 TTTAATGTTT TTTGAGATTA AAAGCTTAAA CAAGAATCTC TAGTTTTCTT TCTTGCTTTT 481 ACTTTTACTT CCTTAATACT CAAGTACAAT TTTAATGGAG TACTTTTTTA CTTTTACTCA 541 AGTAAGATTC TAGCCAGATA CTTTTACTTT TAATTGAGTA AAATTTTCCC TAAGTACTTG 601 TACTTTCACT TGAGTAAAAT TTTTGAGTAC TTTTTACACC TCTGTCAAGA ACTCCTGGAC 661 AAACCTCTGA CCTGTGTGGA ACAGAGTGGA TATGGGTGTC TGAACAGATA TTCACGTCTT 721 TTGCAGATCA GAGGGCATTT CTGGTGTTGT GCAATGACCT GGGTCCAACA GGTAGGCGTA 781 CTCGTATCCA AGAGCATTGT TGGACAGGAG GTAGTCTCCA TTCCTATAAA GAGGAAGGAA 841 TGGCACCATG TAGTAGCCGT CATTGTGGCC AATTGGTGCA TTGGTACGTG GGTAGATGGA 901 CCGGGGGAGG CTGATGAGTT CTTAGCCAGC GCTCAAAGAT GCTAGAAATG ATGTAAGAAA 961 CAAATATGCA AGATGAAAGT TAAGATAAGA TAAAAAAATT TAAACAAATA AATGCAATTT 1021 ATGTTTTGTG TTTCTATTTC CCACCCAATA ATTAACCTCA CGCAGTAAAG CCCACCAGAA 1081 TATTTTTTTG ATCCTTGCTC TGGCAGGCAG TCGCAGCTCG TAACGACTGG AATTCTCCAA 1141 AAT //
Primer: pAxEF_R2 () Sequence file: RDB15276_A7H9d.seq
>D04498I7_A7H9_2_pAxEF_R2_D06_12_ABI24.ab1 1 CGGGGAAAAA AGGCTTTTAT TTGTGAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA 61 TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG 121 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT 181 ATGATCCTCT AGATCAGATC TGCGAAGATA CGGCCACGGG TGCTCTTGAT CCTGTGGCTG 241 ATTTTGGACT GTGCTGCTCG CAGCTGCTGA TGAATCACAT ACTTCCTCCA TTTTCTTCCA 301 CTGATTGACT GTTATAATTT CCCTAATTTC CAGGTCAAGG TGCTGTGCAT TGTGGTAATA 361 GATGTGACAT GACGTCACTT CCAAAGGACC AATGAACATG TCTGACCAAT TTCATATAAT 421 GTGAAAACGA TTTTCATAGG CAGAATAAAT AACATTTAAA TTAAACTGGG CATCAGCGCA 481 ATTCAATTGG TTTGGTAATA GCAAGGGAAA ATAGAATGAA GTGATCTCCA AAAAATAAGT 541 ACTTTTTGAC TGTAAATAAA ATTGTAAGGA GTAAAAAGTA CTTTTTTTTC TAAAAAAATG 601 TAATTAAGTA AAAGTAAAAG TATTGATTTT TAATTGTACT CAAGTAAAGT AAAAATCCCC 661 AAAAATAATA CTTAAGTACA GTAATCAAGT AAAATTACTC AAGTACTTTA CACCTCTGGT 721 TCTTGACCCC CTACCTTCAG CAAGCCCAGC AGATCCACTA GTTCTAGAGC GGCCGCCACC 781 GCGGTGGAGC TCCAGCTTTT GTTCCCTTTA GTGAGGGTTA ATTGCGCGCT TGGCGTAATC 841 ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG 901 AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAA CTCACATTAA 961 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC CTGTCGTGCC AGCTGCATTA 1021 ATGAATCGGC CAACGCGCGG GGAGAGGCCG GTTTGCGTAT GGGCGCTCTT CGCTTTCCTC 1081 GCTTCACTGA ACTTCGGCTG CCGCTCGGGA TCGATTCGGG CCTGGCCGGG CCGTAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. [link to RRC of NBRP]

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Other resources in our bank

zebrafish zGeminin and ortholog

External Database
zebrafish zGeminin

Galaxeidae stony coral GFP

Reference sequence