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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mAG-zGeminin(1/100) / pT2KXIGdeltain

zGem-based S/G2/M marker for zebrafish cells to label nuclei with green fluorescent protein mAG. A component of the zFucci.

Catalog number RDB15276
Resource name mAG-zGeminin(1/100) / pT2KXIGdeltain
Clone info. zGem-based S/G2/M marker for zebrafish cells to label nuclei with green fluorescent protein mAG. A component of the zFucci.
Vector backbone pT2KXIGdeltain (plasmid)
Size of vector backbone 8.1 kb
Selectable markers Amp^r
Gene/insert name zebrafish zGeminin cDNA Galaxeidae stony coral GFP cDNA
Reference of nucleotide sequence AB505860 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
 
Other clones in our bank zebrafish gmnn (KEGG orthology K10749) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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RDB15276 mAG-zGeminin(1/100) / pT2KXIGdeltain DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Transposon and plasmid, Kawakami laboratory (English text)
Featured content Fucci-related materials (en) (English text)
Featured content Fucci-related materials (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15276_A7H9p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F2
Sequence file: RDB15276_A7H9a.seq check
>D04498I7_A7H9_2_EF1a-C_F2_A06_03_ABI24.ab1
    1 GATTGGGGGT TAAGGCCCGG TTCGCTCTCT TCCTCACCGG GTCTGCGGCG AGTTCTAGCT
   61 GAAGCTTCCT GCAGGTCGAC CGATCCTGAG AACTTCAGGG TGAGTTTGGG GACCCTTGAT
  121 TGTTCTTTCT TTTTCGCTAT TGTAAAATTC ATGTTATATG GAGGGGGCAA AGTTTTCAGG
  181 GTGTTGTTTA GAATGGGAAG ATGTCCCTTG TATCACCATG GACCCTCATG ATAATTTTGT
  241 TTCTTTCACT TTCTACTCTG TTGACAACCA TTGTCTCCTC TTATTTTCTT TTCATTTTCT
  301 GTAACTTTTT CGTTAAACTT TAGCTTGCAT TTGTAACGAA TTTTTAAATT CACTTTTGTT
  361 TATTTGTCAG ATTGTAAGTA CTTTCTCTAA TCACTTTTTT TTCAAGGCAA TCAGGGTATA
  421 TTATATTGTA CTTCAGCACA GTTTTAGAGA ACAATTGTTA TAATTAAATG ATAAGGTAGA
  481 ATATTTCTGC ATATAAATTC TGGCTGGCGT GGAAATATTC TTATTGGTAG AAACAACTAC
  541 ACCCTGGTCA TCATCCTGCC TTTCTCTTTA TGGTTACAAT GATATACACT GTTTGAGATG
  601 AGGATAAAAT ACTCTGAGTC CAAACCGGGC CCCTCTGCTA ACCATGTTCA TGCCTTCTTC
  661 TCTTTCCTAC AGCTCCTGGG CAACGTGCTG GTTGTTGTGC TGTCTCATCA TTTTGGCAAA
  721 GAATTCCTCG ACGGATCCAC TAGTAACGGC CGCCAGTGTG CTGGAATTCG CCACCATGGT
  781 GAGCGTGATC AAGCCCGAGA TGAAGATCAA GCTGTGCATG AGGGGCACCG TGAACGGCCA
  841 CAACTTCGTG ATCGAGGGCG AGGGCAAGGG CAACCCCTAC GAGGGCACCC AGATCCTGGA
  901 CCTGAACGTG ACCGAGGGCG CCCCCCCTGC CCTTCGCCTA CGACATCCTG ACCACCGTGT
  961 TCCAGTACGG CAACAGGGCC TTCACCAAGT ACCCCGCCGA CATCCAGGGA CTACTTTCAA
 1021 GCAGACCCTT CCCCGAGGGC TACCAACTGG AGAGGAGCAT GAACCTACGA GGAACAGGCC
 1081 ATCTGCACGC CACCAGCACA TCAGCATGAG GGCGACCTGC CTTCCTCTAC GACAACCAGG
1141 GTTCGA
//
Primer: pAxCALNL_F1
Sequence file: RDB15276_A7H9b.seq check
>D04498I7_A7H9_2_pAxCALNL_F1_B06_06_ABI24.ab1
    1 CTCAATTAAA TCTTATCATG TCTGGATCAT CATCGATTAC TCATAGGCCT CTTGTGTGAC
   61 CCCCTCAGGC TGGTTTTCAT TTTCTGCATT TGTGGATTTG ACAGCCACCT CTGCTTTGAC
  121 TCTCTTTGAA CCCTTAACTT GCTCGGCGCT CCACATCTTC CTTTTGGCCA CTGCTTTACC
  181 ATTCTCAATT ATACGACCAA GGTTTTTGTT TACAGCAGAG GGCTGAAGCA CCTGAAGCGT
  241 CCTCCTCCCC ATTCCTGCTC CAGATGTTGG CGCTACCAGG AATTTCTTGA TGTTTTCAGA
  301 CGGGTTTTCT GCATTCTTTG GTCTTCTGAT GGAACTCATC TCGAGCGGCC GCCAGTGTGA
  361 TGGATATCCC TTGGCCTGGC TGGGCAGCAT GGAGTACCTG GCCACGGCGT TCTCGTACAG
  421 CTTCACCTTG TTGTAGTCCT TGTCGTGCTT CAGGATCTCG ATCCTGTGGT CGATCTTGTG
  481 GGCGTCGGGC AGCCTCACCT CCTTCTTGGC CTTGTAGGTG GTCTTGAAGT CGCACCTGTA
  541 GTGGCCGCCG CCCTCCAGCA GCAGCCTCAT GTTCACGTCG CCCTTCAGCA CGCCGTCCTC
  601 CACGTACATC TTCTCGGTGC TGGGCTCCCA CTTCAGGGTC TTCTTCTGCA TCACGGGGCC
  661 GTTGGGGGGG AAGTTGGTGC CGTCGAACCT GATGTCGTAG AAGAAGCAGT CGCCCCTCAT
  721 GCTGATGTTG CTGGTGGCGG TGCAGATGCC CTGGTCCTCG TAGGTCATGC TCCTCTCCCA
  781 GTGGTAGCCC TCGGGGAAGG TCTGCTTGAA GTAGTCCTGG ATGTCGGCGG GGTACTTGGT
  841 GAAGGCCCTG TTGCCGTACT GGAACACGGT GGTCAGGATG TCGTAGGCGA AGGGCAGGGG
  901 GGCGCCCTCG GTCACGTTCA GGTCCAGGAT CTGGGTGCCC TCGTAGGGTT TGCCCTTGCC
  961 CTCGCCCTCG ATCACGAAGT TGTGGCCGTT CACGGGTGCC CCTCATGCAC AGCTTGATCT
 1021 TCATCTTCGG GCTTGATCAC GCTTCACCAA TGGGTGGGCG AAATTCCAGC ACACTTGGGC
 1081 GGGCCCGTTA CCTAGTGATC CGGTCGGAAG GAAATTTTCT TTTTGACCAA AAATGTGAAT
1141 GAGAGAACCC AGCACAC
//
Primer: EF1a-N_R2
Sequence file: RDB15276_A7H9c.seq check
>D04498I7_A7H9_2_EF1a-N_R2_C06_09_ABI24.ab1
    1 TTACGGGCAA GAATTGCAAG TTTGATTTGC ATTGGAAAGG GTCGCTGGCT TTTGTGTTAC
   61 ACGCCCCTTA TTTGTGCTTG ATTAGATGAT CCCCCTGCTC GAGGGCCCAT CTGGCCTGTG
  121 TTTCAGACAC CAGGGAGTCT CTGCTCACGT TTCCTGCTAT TTGCAGCCTC TCTATCAAGA
  181 CTAATACACC TCTTCCCGCA TCGGCTGCCT GTGAGAGGCT TTTCAGCACT GCAGGATTGC
  241 TTTTCAGCCC CAAAAGAGCT AGGCTTGACA CTAACAATTT TGAGAATCAG CTTCTACTGA
  301 AGTTAAATCT GAGGTTTTAC AACTTTGAGT AGCGTGTACT GGCATTAGAT TGTCTGTCTT
  361 ATAGTTTGAT AATTAAATAC AAACAGTTCT AAAGCAGGAT AAAACCTTGT ATGCATTTCA
  421 TTTAATGTTT TTTGAGATTA AAAGCTTAAA CAAGAATCTC TAGTTTTCTT TCTTGCTTTT
  481 ACTTTTACTT CCTTAATACT CAAGTACAAT TTTAATGGAG TACTTTTTTA CTTTTACTCA
  541 AGTAAGATTC TAGCCAGATA CTTTTACTTT TAATTGAGTA AAATTTTCCC TAAGTACTTG
  601 TACTTTCACT TGAGTAAAAT TTTTGAGTAC TTTTTACACC TCTGTCAAGA ACTCCTGGAC
  661 AAACCTCTGA CCTGTGTGGA ACAGAGTGGA TATGGGTGTC TGAACAGATA TTCACGTCTT
  721 TTGCAGATCA GAGGGCATTT CTGGTGTTGT GCAATGACCT GGGTCCAACA GGTAGGCGTA
  781 CTCGTATCCA AGAGCATTGT TGGACAGGAG GTAGTCTCCA TTCCTATAAA GAGGAAGGAA
  841 TGGCACCATG TAGTAGCCGT CATTGTGGCC AATTGGTGCA TTGGTACGTG GGTAGATGGA
  901 CCGGGGGAGG CTGATGAGTT CTTAGCCAGC GCTCAAAGAT GCTAGAAATG ATGTAAGAAA
  961 CAAATATGCA AGATGAAAGT TAAGATAAGA TAAAAAAATT TAAACAAATA AATGCAATTT
 1021 ATGTTTTGTG TTTCTATTTC CCACCCAATA ATTAACCTCA CGCAGTAAAG CCCACCAGAA
 1081 TATTTTTTTG ATCCTTGCTC TGGCAGGCAG TCGCAGCTCG TAACGACTGG AATTCTCCAA
1141 AAT
//
Primer: pAxEF_R2
Sequence file: RDB15276_A7H9d.seq check
>D04498I7_A7H9_2_pAxEF_R2_D06_12_ABI24.ab1
    1 CGGGGAAAAA AGGCTTTTAT TTGTGAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA
   61 TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG
  121 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT
  181 ATGATCCTCT AGATCAGATC TGCGAAGATA CGGCCACGGG TGCTCTTGAT CCTGTGGCTG
  241 ATTTTGGACT GTGCTGCTCG CAGCTGCTGA TGAATCACAT ACTTCCTCCA TTTTCTTCCA
  301 CTGATTGACT GTTATAATTT CCCTAATTTC CAGGTCAAGG TGCTGTGCAT TGTGGTAATA
  361 GATGTGACAT GACGTCACTT CCAAAGGACC AATGAACATG TCTGACCAAT TTCATATAAT
  421 GTGAAAACGA TTTTCATAGG CAGAATAAAT AACATTTAAA TTAAACTGGG CATCAGCGCA
  481 ATTCAATTGG TTTGGTAATA GCAAGGGAAA ATAGAATGAA GTGATCTCCA AAAAATAAGT
  541 ACTTTTTGAC TGTAAATAAA ATTGTAAGGA GTAAAAAGTA CTTTTTTTTC TAAAAAAATG
  601 TAATTAAGTA AAAGTAAAAG TATTGATTTT TAATTGTACT CAAGTAAAGT AAAAATCCCC
  661 AAAAATAATA CTTAAGTACA GTAATCAAGT AAAATTACTC AAGTACTTTA CACCTCTGGT
  721 TCTTGACCCC CTACCTTCAG CAAGCCCAGC AGATCCACTA GTTCTAGAGC GGCCGCCACC
  781 GCGGTGGAGC TCCAGCTTTT GTTCCCTTTA GTGAGGGTTA ATTGCGCGCT TGGCGTAATC
  841 ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG
  901 AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAA CTCACATTAA
  961 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC CTGTCGTGCC AGCTGCATTA
 1021 ATGAATCGGC CAACGCGCGG GGAGAGGCCG GTTTGCGTAT GGGCGCTCTT CGCTTTCCTC
1081 GCTTCACTGA ACTTCGGCTG CCGCTCGGGA TCGATTCGGG CCTGGCCGGG CCGTAG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430.

2021.02.18

GNP_filter3_RDBDEP_html_201222.pl