RDB15262 - Mermaid

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Mermaid (#RDB15262)

Voltage indicator Mermaid.

Clone info.	Voltage indicator Mermaid.
Comment	For the compornent of Mermaid, refer Fig.S4 of Tsutsui, H., Nat. Methods 5 (8): 683-685, 2008. The nucleotide sequence of Umi-Kinoko Green florescent protein has been deposited as AB425088.
Vector backbone	pCS2+ (Plasmid)
Gene/insert name	Ciona intestinalis Ci-VSP cDNA Sarcophyton sp. GFP (Umikinoko-green) cDNA Fungia concinna OFP (Kusabira Orange) cDNA
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Tsutsui, H. et al., Nat. Methods 5 (8): 683-685 (2008).
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Tsutsui, H. et al., Nat. Methods 5 (8): 683-685 (2008). Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Tsutsui, H. et al., Nat. Methods 5 (8): 683-685 (2008). 付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。
備考	提供条件は英文のまま記載してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15262	Mermaid	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Mermaid was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15262).

Reference section:

Tsutsui, H., Karasawa, S., Okamura, Y., Miyawaki, A., Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685 (2008). PMID 18622396. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15262_A7H9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F Sequence file: RDB15262_A7H9a.seq
>D04498H2_2_A7H9_pCMV-IE94_F_A01_01_ABI08.ab1 1 GTGGGCGGGG AAGCGGTGCT AATGGGAGGT CTATATAAGC AATGCTCGTT TAGGGAACCG 61 CCATTCTGCC TGGGGACGTC GGAGCTAGCT TGATTTAGGT GACACTATAG AATACAAGCT 121 TATGGAGGGA TTCGACGGTT CAGATTTTAG TCCTCCAGCT GATTTAGTTG GCGTTGACGG 181 TGCAGTCATG CGGAACGTCG TTGACGTCAC GATAAATGGT GACGTCACTG CTCCGCCGAA 241 AGCTGCGCCA AGAAAAAGTG AATCGGTAAA GAAAGTTCAT TGGAATGATG TAGACCAAGG 301 ACCGAGTGAA AAACCAGAGA CAAGACAGGA GGAACGAATA GATATACCCG AGATTTCAGG 361 TCTATGGTGG GGCGAGAATG AACATGGAGT GGACGATGGG AGAATGGAGA TACCTACTAC 421 TGGTGTAGGT CGCGTCCAGT TTCGTGTCCG AGCAGTGATT GATCATCTAG GGATGCGAGT 481 CTTTGGAGTC TTCCTAATTT TCTTGGACAT CATCCTCATG ATCATTGATC TCAGTCTTCC 541 AGGAAAAAGT GAATCTTCAC AATCCTTTTA TGACGGGATG GCTTTGGCTC TTTCTTGTTA 601 TTTCATGCTG GATTTAGGAT TAAGGATATT TGCCTACGGG CCCAAGAATT TCTTCACCAA 661 CCCCTGGGAG GTTGCTGATG GTTTGATTAT CGTTGTCACA TTCGTCGTCA CGATATTTTA 721 CACTGTGTTA GATGAATACG TGCAAGAAAC AGGAGCCGAT GGTTTGGGGC AATTGGTTGT 781 GTTGGCCCGT TTGCTGCGTG TGGTTCGATT AGCAAGAATA TTTTATTCCC ACCAACAAAT 841 GAAGGCTTCA AGCAGAAGAA CAATATCAGG GGATCCCATG AGTGTGATTA AAGAGGAAAT 901 GAAGATCAAG CTGCATATGG AAGGAAATGT AAACGGTCAT GCATTTGTGA TTGAAAGGAG 961 ATGGGAAAAG GGAAAGCCCT TACGATGGGA CACAGACTTT TAAACCCTGA CAGTGAAAGA 1021 AGCCGCAACC TCTCCCTTTT TCTTTACGAC CATCTGACCA ATGCGTTCCA GTTACCGAAA 1081 TAGAGCAATT CACTAAATAT TCCAGGACCG G //
Primer: mKO2-N_R Sequence file: RDB15262_A7H9b.seq
>D04498H2_2_A7H9_mKO2-N_R_B04_02_ABI08.ab1 1 GAATCTCATT GTGACTCATG CCCATTGACG GAGCCGTCCA TGTAGTACCT CATCTTCATC 61 TCTGGTTTAA TCACACTCAC CATGGTCTCG AGCTTCGAAG CCTGACTTGG CAGCAAAGAA 121 TTGCGAGCAA CCGCATTCTC ATACAGCTTG ACTTTGCTGT AATCCCTGTC ATGGCTCAAG 181 ATCTCGATAC GATGGTCGAT CTTGTGGGCA TCTGGCAACT GCACAACCTT TTTCGCTTTG 241 TAGGAAGTTT TGAAGTCACA TCGGTGATGG CCACCTCCTT CAAGCAACAG AGCCATGTTA 301 ACATCACCCA TCAGAAACCC ATCACGCACG TACATCATCT CAGTGGATGG TTCCCACTTC 361 AAAGTTTTCT TCTGCATAAC TGGACCATTG GGGGGAAAGT TCTTGCCATC AAACCGAATT 421 TTATAGATAA AGCAGTCGCC TTCCATGCTG ATCTCGCTTC TCACGTTGCA AATGGCGTTG 481 TCTTCATAAC TCATGGTTCT TTCCCATGAA TACCCCTCGG GAAACGTCTG CTTGAAATAG 541 TCTGGTATAT CGGCTGGATA TTTAGTGAAT GCTCTATTTC CGTACTGGAA CGCATTTGTC 601 AAGATGTCGT AAGAAAAAGG GAGAGGTGCG CCTTCTTTCA CTGTCAGGTT TAAAGTCTGT 661 GTCCCATCGT AAGGCTTTCC TTTTCCATCT CCTTCAATCA CAAATGCATG ACCGTTTACA 721 TTTCCTTCCA TATGCAGCTT GATCTTCATT TCCTCTTTAA TCACACTCAT GGGATCCCCT 781 GATATTGTTC TTCTGCTTGA AGCCTTCATT TGTTGGTGGG AATAAAATAT TCTTGCTAAT 841 CGAACCACAC GCAGCAAACG GGCCAACACA ACCAATTGCC CCAAAACCAT CGGCTCCTGT 901 TTCTTGCACG TATTCATCTA ACACAGTGTA AAATATCGTG ACGACGAATG TGACAACGAT 961 AATCAAACCA TCAGCAACCC TCCCAGGGGT TTGGTGAAGA ATTTCTTGGG CCGTAGGCAA 1021 ATTATCCTTA ATCCCTAAAT CCAGCATGAA TAACCAAGGA AAGAGCCAAA GCCCATTCCC 1081 GTCATAAAGA TGTGAGATCA CTTTTTTCCT GGGAAGAACT //
Primer: pAxCALNL_F1 Sequence file: RDB15262_A7H9c.seq
>D04498H2_A7H9_2_pAxCALNL_F1_G04_19_ABI24.ab1 1 TTCATGTATC TTATCATGTC TGGATCTACG TAATACGACT CACTATAGTT CTAGATTAGG 61 AATGAGCTAC TGCATCTTCT ACCTGCTCAG TAATGTTGCC TTCGGTTTTC CTGACGAGGC 121 GATGGCCGAT GTAATGGTCT CCTGGCATTT CAAGAATCTC TTTTGCCGCC TTGTAAGTAG 181 TCTTGAATTG GCATTTGTGA TTGCCGCCTC CTTCAAGTTT TAGGTACATC GTAACATCAC 241 CCTTCAGAAC TCCGTCGCTG GCAGTAATTT TCTCGGTTGA TGGCTCCCAA TCAACACTTT 301 GGTTTTGCAT GATAGGACCA TCGGCAGGAA AGTTAACCCC AGTAAATTTG GATTTGTGGT 361 AGAAGGTGTT TCCTCTAAGG CTTATATGCG CACTGACTGA AGCGGACCCA CCATCTTCGA 421 ACTCCAACGA CCTTTCCCAT GACAGGCCTT CAGGAAATGC TTGTTTGAAA TAGTCTGGTA 481 TCTCTTCTGG ATATTTAGTA AATACTCTGT GGCCGTAACA GAACACGTGT GACACTAAGT 541 CAAACGCGAA AGGCATTGGC CCGCCCTCGG CCATTGTGAC GCGTAGTGTC ATCTCTTGAT 601 GTCCCTCGTA AGGTCTGCCT GTGCCTTCAC CTTCAATTGT GAACTCATGC CCATTGACGG 661 AGCCGTCCAT GTAGTACCTC ATCTTCATCT CTGGTTTAAT CACACTCACC ATGGTCTCGA 721 GCTTCGAAGC CTGACTTGGC AGCAAAGAAT TGCGAGCACC GCATTCTCAT ACAGCTTGAC 781 TTTGCTGTAT CCCTGTCATG GCTCAGATCT CGATACGATG GTCGATCTTG TGGGCATCTG 841 GCACTGCAAC ACCTTTTTCG CTTTGTAGAG TTTGAGTCAC ATCGTGATGG CACTCTCCAG 901 CCACAGAGCC ATGGTAACAT CACCCATCGA AACCATCACG CACGGTACTC ATCTCCATGA 961 TGTCACTTCA GTTTCTTCGC TGACTGCACA TTGGGCAAGT TCTTGCACTA CGATTATAGT 1021 AGCGGTGCTC T //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Tsutsui, H., Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685 (2008). PMID 18622396. [link to RRC of NBRP]