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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Mermaid

Voltage indicator Mermaid.

Catalog number RDB15262
Resource name Mermaid
Clone info. Voltage indicator Mermaid.
For the compornent of Mermaid, refer Fig.S4 of Tsutsui, H., Nat. Methods 5 (8): 683-685, 2008. The nucleotide sequence of Umi-Kinoko Green florescent protein has been deposited as AB425088.
Vector backbone pCS2+ (Plasmid)
Gene/insert name Ciona intestinalis Ci-VSP cDNA Sarcophyton sp. GFP (Umikinoko-green) cDNA Fungia concinna OFP (Kusabira Orange) cDNA
Depositor|Developer Miyawaki, Atsushi |
 
Other clones in our bank Ciona intestinalis vsp (KEGG orthology K18079) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Tsutsui H, Karasawa S, Okamura Y, Miyawaki A. (2008) Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
Ordering Please visit Information of Request for Distribution.[link] 
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15262 Mermaid DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Tsutsui, H., Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685 (2008). PMID 18622396.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Sensor and Visualization (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15262_A7H9p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F
Sequence file: RDB15262_A7H9a.seq check
>D04498H2_2_A7H9_pCMV-IE94_F_A01_01_ABI08.ab1
    1 GTGGGCGGGG AAGCGGTGCT AATGGGAGGT CTATATAAGC AATGCTCGTT TAGGGAACCG
   61 CCATTCTGCC TGGGGACGTC GGAGCTAGCT TGATTTAGGT GACACTATAG AATACAAGCT
  121 TATGGAGGGA TTCGACGGTT CAGATTTTAG TCCTCCAGCT GATTTAGTTG GCGTTGACGG
  181 TGCAGTCATG CGGAACGTCG TTGACGTCAC GATAAATGGT GACGTCACTG CTCCGCCGAA
  241 AGCTGCGCCA AGAAAAAGTG AATCGGTAAA GAAAGTTCAT TGGAATGATG TAGACCAAGG
  301 ACCGAGTGAA AAACCAGAGA CAAGACAGGA GGAACGAATA GATATACCCG AGATTTCAGG
  361 TCTATGGTGG GGCGAGAATG AACATGGAGT GGACGATGGG AGAATGGAGA TACCTACTAC
  421 TGGTGTAGGT CGCGTCCAGT TTCGTGTCCG AGCAGTGATT GATCATCTAG GGATGCGAGT
  481 CTTTGGAGTC TTCCTAATTT TCTTGGACAT CATCCTCATG ATCATTGATC TCAGTCTTCC
  541 AGGAAAAAGT GAATCTTCAC AATCCTTTTA TGACGGGATG GCTTTGGCTC TTTCTTGTTA
  601 TTTCATGCTG GATTTAGGAT TAAGGATATT TGCCTACGGG CCCAAGAATT TCTTCACCAA
  661 CCCCTGGGAG GTTGCTGATG GTTTGATTAT CGTTGTCACA TTCGTCGTCA CGATATTTTA
  721 CACTGTGTTA GATGAATACG TGCAAGAAAC AGGAGCCGAT GGTTTGGGGC AATTGGTTGT
  781 GTTGGCCCGT TTGCTGCGTG TGGTTCGATT AGCAAGAATA TTTTATTCCC ACCAACAAAT
  841 GAAGGCTTCA AGCAGAAGAA CAATATCAGG GGATCCCATG AGTGTGATTA AAGAGGAAAT
  901 GAAGATCAAG CTGCATATGG AAGGAAATGT AAACGGTCAT GCATTTGTGA TTGAAAGGAG
  961 ATGGGAAAAG GGAAAGCCCT TACGATGGGA CACAGACTTT TAAACCCTGA CAGTGAAAGA
 1021 AGCCGCAACC TCTCCCTTTT TCTTTACGAC CATCTGACCA ATGCGTTCCA GTTACCGAAA
1081 TAGAGCAATT CACTAAATAT TCCAGGACCG G
//
Primer: mKO2-N_R
Sequence file: RDB15262_A7H9b.seq check
>D04498H2_2_A7H9_mKO2-N_R_B04_02_ABI08.ab1
    1 GAATCTCATT GTGACTCATG CCCATTGACG GAGCCGTCCA TGTAGTACCT CATCTTCATC
   61 TCTGGTTTAA TCACACTCAC CATGGTCTCG AGCTTCGAAG CCTGACTTGG CAGCAAAGAA
  121 TTGCGAGCAA CCGCATTCTC ATACAGCTTG ACTTTGCTGT AATCCCTGTC ATGGCTCAAG
  181 ATCTCGATAC GATGGTCGAT CTTGTGGGCA TCTGGCAACT GCACAACCTT TTTCGCTTTG
  241 TAGGAAGTTT TGAAGTCACA TCGGTGATGG CCACCTCCTT CAAGCAACAG AGCCATGTTA
  301 ACATCACCCA TCAGAAACCC ATCACGCACG TACATCATCT CAGTGGATGG TTCCCACTTC
  361 AAAGTTTTCT TCTGCATAAC TGGACCATTG GGGGGAAAGT TCTTGCCATC AAACCGAATT
  421 TTATAGATAA AGCAGTCGCC TTCCATGCTG ATCTCGCTTC TCACGTTGCA AATGGCGTTG
  481 TCTTCATAAC TCATGGTTCT TTCCCATGAA TACCCCTCGG GAAACGTCTG CTTGAAATAG
  541 TCTGGTATAT CGGCTGGATA TTTAGTGAAT GCTCTATTTC CGTACTGGAA CGCATTTGTC
  601 AAGATGTCGT AAGAAAAAGG GAGAGGTGCG CCTTCTTTCA CTGTCAGGTT TAAAGTCTGT
  661 GTCCCATCGT AAGGCTTTCC TTTTCCATCT CCTTCAATCA CAAATGCATG ACCGTTTACA
  721 TTTCCTTCCA TATGCAGCTT GATCTTCATT TCCTCTTTAA TCACACTCAT GGGATCCCCT
  781 GATATTGTTC TTCTGCTTGA AGCCTTCATT TGTTGGTGGG AATAAAATAT TCTTGCTAAT
  841 CGAACCACAC GCAGCAAACG GGCCAACACA ACCAATTGCC CCAAAACCAT CGGCTCCTGT
  901 TTCTTGCACG TATTCATCTA ACACAGTGTA AAATATCGTG ACGACGAATG TGACAACGAT
  961 AATCAAACCA TCAGCAACCC TCCCAGGGGT TTGGTGAAGA ATTTCTTGGG CCGTAGGCAA
 1021 ATTATCCTTA ATCCCTAAAT CCAGCATGAA TAACCAAGGA AAGAGCCAAA GCCCATTCCC
1081 GTCATAAAGA TGTGAGATCA CTTTTTTCCT GGGAAGAACT
//
Primer: pAxCALNL_F1
Sequence file: RDB15262_A7H9c.seq check
>D04498H2_A7H9_2_pAxCALNL_F1_G04_19_ABI24.ab1
    1 TTCATGTATC TTATCATGTC TGGATCTACG TAATACGACT CACTATAGTT CTAGATTAGG
   61 AATGAGCTAC TGCATCTTCT ACCTGCTCAG TAATGTTGCC TTCGGTTTTC CTGACGAGGC
  121 GATGGCCGAT GTAATGGTCT CCTGGCATTT CAAGAATCTC TTTTGCCGCC TTGTAAGTAG
  181 TCTTGAATTG GCATTTGTGA TTGCCGCCTC CTTCAAGTTT TAGGTACATC GTAACATCAC
  241 CCTTCAGAAC TCCGTCGCTG GCAGTAATTT TCTCGGTTGA TGGCTCCCAA TCAACACTTT
  301 GGTTTTGCAT GATAGGACCA TCGGCAGGAA AGTTAACCCC AGTAAATTTG GATTTGTGGT
  361 AGAAGGTGTT TCCTCTAAGG CTTATATGCG CACTGACTGA AGCGGACCCA CCATCTTCGA
  421 ACTCCAACGA CCTTTCCCAT GACAGGCCTT CAGGAAATGC TTGTTTGAAA TAGTCTGGTA
  481 TCTCTTCTGG ATATTTAGTA AATACTCTGT GGCCGTAACA GAACACGTGT GACACTAAGT
  541 CAAACGCGAA AGGCATTGGC CCGCCCTCGG CCATTGTGAC GCGTAGTGTC ATCTCTTGAT
  601 GTCCCTCGTA AGGTCTGCCT GTGCCTTCAC CTTCAATTGT GAACTCATGC CCATTGACGG
  661 AGCCGTCCAT GTAGTACCTC ATCTTCATCT CTGGTTTAAT CACACTCACC ATGGTCTCGA
  721 GCTTCGAAGC CTGACTTGGC AGCAAAGAAT TGCGAGCACC GCATTCTCAT ACAGCTTGAC
  781 TTTGCTGTAT CCCTGTCATG GCTCAGATCT CGATACGATG GTCGATCTTG TGGGCATCTG
  841 GCACTGCAAC ACCTTTTTCG CTTTGTAGAG TTTGAGTCAC ATCGTGATGG CACTCTCCAG
  901 CCACAGAGCC ATGGTAACAT CACCCATCGA AACCATCACG CACGGTACTC ATCTCCATGA
  961 TGTCACTTCA GTTTCTTCGC TGACTGCACA TTGGGCAAGT TCTTGCACTA CGATTATAGT
1021 AGCGGTGCTC T
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Tsutsui, H., Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685 (2008). PMID 18622396.

2021.08.13

GNP_filter3_RDBDEP_html_210618.pl