RDB15234 - dKeima/pRSETB

Catalog number	RDB15234
Resource name	dKeima/pRSETB
Clone info.	Plasmd clone of dimeric Keima-Red (dKeima).
Vector backbone	Plasmid
Depositor\|Developer	Miyawaki, Atsushi \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, Miyawaki A. (2006) A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

Distribution information (domestic users)

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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, Miyawaki A. (2006) A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15234	dKeima/pRSETB	DNA solution

Electronic file	Nucleotide sequence (partial) compiled by us RDB15234pta01.seq

original	Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

Featured content	Fluorescent protein resources (English text)
Featured content	Fluorescent protein resources (Japanese text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: lacIpro-B_F Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5' Sequence file: RDB15234_A7H9a.seq
>15234_B0L4F_1_lacIpro-B_F_G01_19_ABI24.ab1 1 NNNNNNNNNN NNNNNNCGCA NCGNNTCNGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG 61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGGATCTCGA TCCCGCGAAA 121 TTAATACGAC TCACTATAGG GAGACCACAA CGGTTTCCCT CTAGAAATAA TTTTGTTTAA 181 CTTTAAGAAG GAGATATACA TATGCGGGGT TCTCATCATC ATCATCATCA TGGTATGGCT 241 AGCATGACTG GTGGACAGCA AATGGGTCGG GATCTGTACG ACGATGACGA TAAGGATCCG 301 ACCATGGTGA GTGTGATCGC TAAACAAATG ACCTACAAGG TTTATATGTC AGGCACGGTC 361 AATGGACACT ACTTTGAGGT CGAAGGCGAT GGAAAAGGAA AGCCTTACGA GGGAGAGCAG 421 ACAGTAAAGC TCACTGTCAC CAAGGGTGGA CCTCTGCCAT TTGCTTGGGA TATATTATCA 481 CCACTGTTTC AGTACGGAAG CATACCATTC ACCAAGTACC CTGAAGACAT CCCTGATTAT 541 GTAAAGCAGT CATTCCCTGA GGGATATACA TGGGAGAGGA CCATGAACTT TGAAGATGGT 601 GCAGTGTGTA CTGTCAGCAA TGATTCCAGC ATCCAAGGCA ACTGTTTCAT CTACAATGTC 661 AAAATCTCTG GTACGAACTT TCCTCCCAAT GGACCTGTTA TGCAGAAGAA GACACAGGGC 721 TGGGAACCCA GCACTGAGCG TCTCTTTGCA CGAGATGGAA TGCTGATAGG AAACGATTAT 781 ATGGCTCTGA AGTTGGAAGG AGGTGGTCAC TATTTGTGTG AATTTAAATC TACTTACAAG 841 GCAAAGAANC CTGTGANGAT GNCAGGGTAT CACTATATTG ACCGCAAACT GGATGNNNNC 901 AGTCACNACA GGGNNTANAC ATCTGNTGAN CANNGTGAAA TANCCNNTGN ACGCCACTCT 961 TTGNTCGGNN GNNTACCTTT CNGANTTAAA TCNNNNNANN NNANCCANNN NNNTAAAAAN 1021 NGTANATTTN NNTTTTANNN NNNANNNNNN NNNN //
Primer: T7ter_R1 Region: insert 3' Sequence file: RDB15234_A7H9b.seq
>15234_B0L4F_1_T7ter_R1_H01_22_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NGTNNNTGNT NNGCGGTGGC AGCAGCCAAC TCAGCTTCCT 61 TTCGGGCTTT GTTAGCAGCC GGATCAAGCT TCGAATTCGC GGCCGCAGGA ATTTTTTTTT 121 TTTTTTTGTT TTCAAAAGTT TAATCACTGA AGGTTGTTTA CACTTCTTCG TTCATACTTC 181 TCTAAGCTAA AATCAAAATC TACGTTTTTT TACGCCACTG GGTATTTCTG CCTTGATTTA 241 ACTCCGAAAG GTAGTCAACC GAGCAAAGAG TGGCGTGCAA TGGCTATTTC ACACTGCTCA 301 ACAGATGTGT AATCCCTGTT GTGACTGGTT ACATCCAGTT TGCGGTCAAT ATAGTGATAC 361 CCTGGCATCC TCACAGGCTT CTTTGCCTTG TAAGTAGATT TAAATTCACA CAAATAGTGA 421 CCACCTCCTT CCAACTTCAN AGCCATATAA TCGTTTCCTA TCAGCATTCC ATCTCGTGCA 481 AAGAGACGCT CAGTGCTGGG TTCCCAGCCC TGTGTCTTCT TCTGCATAAC AGGTCCATTG 541 GGAGGAAAGT TCGTACCANA NATTTTGACA TTGTANATGA AACAGTTGCC TTGGATGCTG 601 GAATCATTGC TGACAGTACA CACTGCACCA TCTTCAAAGT TCATGGTCCT CTCCCATGTA 661 TATCCCTCAG GGAATGACTG CTTTACATAA TCAGGGATGT CTTCAGGGTA CTTGGTGAAT 721 GGTATGCTTC CGTACTGAAA CAGTGGTGAT AATATATCCC AAGCAAATGG CAGANGTCCA 781 CCCTTGGTGA CAGTGAGCTT TACTGTCTGC TCTCNCTCGT AANGNTTTCC TTTTCCNTCG 841 CCTTCNACCT CAAAGTAGTG NCCATTGACC GTGCCTGACA TATAAACCTT GNANNNCATT 901 TGNTTANCGA TCACACTCNC CATGGTCGGA TCCTTATCGN CNTCGTCGTA CAGATCCCGA 961 NCNNTTTGCT GNNCACCAGN CATGNTANCC ATANCATGNN GANGANGNNG ANGANAANCC 1021 CNNNNATGTA NNTNNNNNTN NNNNANNNNN ANNNAANNNN NNTNNNNNNN GNNANNNNNN 1081 NNNN //