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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

dKeima/pRSETB

Plasmd clone of dimeric Keima-Red (dKeima).

Catalog number RDB15234
Resource name dKeima/pRSETB
Clone info. Plasmd clone of dimeric Keima-Red (dKeima).
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, Miyawaki A. (2006) A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15234 dKeima/pRSETB DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence (partial) RDB15234pta01.seq compiled by us

Original reference

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15234_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F
Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
Sequence file: RDB15234_A7H9a.seq check
>15234_B0L4F_1_lacIpro-B_F_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCGCA NCGNNTCNGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG
   61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGGATCTCGA TCCCGCGAAA
  121 TTAATACGAC TCACTATAGG GAGACCACAA CGGTTTCCCT CTAGAAATAA TTTTGTTTAA
  181 CTTTAAGAAG GAGATATACA TATGCGGGGT TCTCATCATC ATCATCATCA TGGTATGGCT
  241 AGCATGACTG GTGGACAGCA AATGGGTCGG GATCTGTACG ACGATGACGA TAAGGATCCG
  301 ACCATGGTGA GTGTGATCGC TAAACAAATG ACCTACAAGG TTTATATGTC AGGCACGGTC
  361 AATGGACACT ACTTTGAGGT CGAAGGCGAT GGAAAAGGAA AGCCTTACGA GGGAGAGCAG
  421 ACAGTAAAGC TCACTGTCAC CAAGGGTGGA CCTCTGCCAT TTGCTTGGGA TATATTATCA
  481 CCACTGTTTC AGTACGGAAG CATACCATTC ACCAAGTACC CTGAAGACAT CCCTGATTAT
  541 GTAAAGCAGT CATTCCCTGA GGGATATACA TGGGAGAGGA CCATGAACTT TGAAGATGGT
  601 GCAGTGTGTA CTGTCAGCAA TGATTCCAGC ATCCAAGGCA ACTGTTTCAT CTACAATGTC
  661 AAAATCTCTG GTACGAACTT TCCTCCCAAT GGACCTGTTA TGCAGAAGAA GACACAGGGC
  721 TGGGAACCCA GCACTGAGCG TCTCTTTGCA CGAGATGGAA TGCTGATAGG AAACGATTAT
  781 ATGGCTCTGA AGTTGGAAGG AGGTGGTCAC TATTTGTGTG AATTTAAATC TACTTACAAG
  841 GCAAAGAANC CTGTGANGAT GNCAGGGTAT CACTATATTG ACCGCAAACT GGATGNNNNC
  901 AGTCACNACA GGGNNTANAC ATCTGNTGAN CANNGTGAAA TANCCNNTGN ACGCCACTCT
  961 TTGNTCGGNN GNNTACCTTT CNGANTTAAA TCNNNNNANN NNANCCANNN NNNTAAAAAN
1021 NGTANATTTN NNTTTTANNN NNNANNNNNN NNNN
//
Primer: T7ter_R1
Region: insert 3'
Sequence file: RDB15234_A7H9b.seq check
>15234_B0L4F_1_T7ter_R1_H01_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGTNNNTGNT NNGCGGTGGC AGCAGCCAAC TCAGCTTCCT
   61 TTCGGGCTTT GTTAGCAGCC GGATCAAGCT TCGAATTCGC GGCCGCAGGA ATTTTTTTTT
  121 TTTTTTTGTT TTCAAAAGTT TAATCACTGA AGGTTGTTTA CACTTCTTCG TTCATACTTC
  181 TCTAAGCTAA AATCAAAATC TACGTTTTTT TACGCCACTG GGTATTTCTG CCTTGATTTA
  241 ACTCCGAAAG GTAGTCAACC GAGCAAAGAG TGGCGTGCAA TGGCTATTTC ACACTGCTCA
  301 ACAGATGTGT AATCCCTGTT GTGACTGGTT ACATCCAGTT TGCGGTCAAT ATAGTGATAC
  361 CCTGGCATCC TCACAGGCTT CTTTGCCTTG TAAGTAGATT TAAATTCACA CAAATAGTGA
  421 CCACCTCCTT CCAACTTCAN AGCCATATAA TCGTTTCCTA TCAGCATTCC ATCTCGTGCA
  481 AAGAGACGCT CAGTGCTGGG TTCCCAGCCC TGTGTCTTCT TCTGCATAAC AGGTCCATTG
  541 GGAGGAAAGT TCGTACCANA NATTTTGACA TTGTANATGA AACAGTTGCC TTGGATGCTG
  601 GAATCATTGC TGACAGTACA CACTGCACCA TCTTCAAAGT TCATGGTCCT CTCCCATGTA
  661 TATCCCTCAG GGAATGACTG CTTTACATAA TCAGGGATGT CTTCAGGGTA CTTGGTGAAT
  721 GGTATGCTTC CGTACTGAAA CAGTGGTGAT AATATATCCC AAGCAAATGG CAGANGTCCA
  781 CCCTTGGTGA CAGTGAGCTT TACTGTCTGC TCTCNCTCGT AANGNTTTCC TTTTCCNTCG
  841 CCTTCNACCT CAAAGTAGTG NCCATTGACC GTGCCTGACA TATAAACCTT GNANNNCATT
  901 TGNTTANCGA TCACACTCNC CATGGTCGGA TCCTTATCGN CNTCGTCGTA CAGATCCCGA
  961 NCNNTTTGCT GNNCACCAGN CATGNTANCC ATANCATGNN GANGANGNNG ANGANAANCC
 1021 CNNNNATGTA NNTNNNNNTN NNNNANNNNN ANNNAANNNN NNTNNNNNNN GNNANNNNNN
1081 NNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

2021.08.13

GNP_filter3_RDBDEP_html_210618.pl