Please review the QC test results indicated by icon below as well as clone information before placing your order.

UnaG/pcDNA3-FLAG

Expression vector of eel UnaG, bilirubin-inducible fluorescent protein.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Kumagai, A., Ando. R,, Miyatake, H., Greimel, P., Kobayashi, T., Hirabayashi, Y., Shimogori, T., Miyawaki, A. (2013) A Bilirubin-Inducible Fluorescent Protein from Eel Muscle. Cell, 153 (7): 1602-1611. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	((Additional form)) Approval Form(FormD) Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Kumagai, A., Ando. R,, Miyatake, H., Greimel, P., Kobayashi, T., Hirabayashi, Y., Shimogori, T., Miyawaki, A. (2013) A Bilirubin-Inducible Fluorescent Protein from Eel Muscle. Cell, 153 (7): 1602-1611. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	【追加提供依頼書】承諾書(FormD) 提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15703_A7Lcp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5'(FLAG,UnaG 5') Sequence file: RDB15703_A7Lcc.seq
>D05018C7_A7Lc_2_CMV-Forward_D02_11_ABI24.ab1     1 NNNNNNNNNN NCCNTNNNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC   121 TCACTATAGG GAGACCCAAG CTTGCCACCA TGGATTACAA GGATGACGAC GATAAGGGAT   181 CCGGTGGTTC TGGTATGGTC GAGAAATTTG TTGGCACCTG GAAGATCGCA GACAGCCATA   241 ATTTTGGTGA ATACCTGAAA GCTATCGGAG CCCCAAAGGA ATTAAGCGAT GGTGGGGATG   301 CCACGACGCC GACATTGTAC ATCTCCCAGA AGGACGGAGA CAAAATGACA GTGAAAATAG   361 AGAATGGACC TCCTACGTTC CTTGACACTC AAGTAAAGTT CAAATTAGGG GAGGAGTTCG   421 ACGAATTTCC TTCTGATCGA AGAAAAGGCG TAAAATCTGT CGTGAACTTG GTGGGAGAGA   481 AGCTGGTGTA CGTACAAAAG TGGGACGGCA AGGAGACGAC GTATGTCCGA GAGATAAAGG   541 ACGGTAAACT GGTCGTGACA CTTACGATGG GAGACGTCGT GGCTGTGCGC AGCTACCGGA   601 NGGCGACGGA ATGAGAATTC TGCAGATAGC TAGCTAGCTA TCCATCACAC TGGCGGCCGC   661 TCGAGCATGC ATCTAGAGGG CCCTATTCTA TAGTGTCACC TAAATGCTAG AGCTCGCTGA   721 TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC NCCCGTGCCT   781 TCCTTGACCC TGGAANGTGC CACTCNNACT GTCCTTTCCT AATAAAATGA NGAAATTGCA   841 TCGCATNNNC TGANTANGNG TCATTCTATT CTGGGGGNNN GGGGNGGGGN NANGNNANCN   901 ANGGGGGANN NNTGGGGNAN NANNNNNNCN NNNNTGCNGG GGNNNGCNGN NNGGNNNNNN 961 NNNN //
Primer: BGH_rev2 (Pr0606) Region: bGH pA,insert(UnaG,FLAG) Sequence file: RDB15703_A7Lcd.seq
>15703_15703_B3BeF_BGH_rev2_H01_22_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GGNNGNNGGG CNNACNACAG ATGGCTGGCA ACTAGAAGGC    61 ACAGTCGAGG CTGATCAGCG AGCTCTAGCA TTTAGGTGAC ACTATAGAAT AGGGCCCTCT   121 AGATGCATGC TCGAGCGGCC GCCAGTGTGA TGGATAGCTA GCTAGCTATC TGCAGAATTC   181 TCATTCCGTC GCCCTCCGGT AGCTGCGCAC AGCCACGACG TCTCCCATCG TAAGTGTCAC   241 GACCAGTTTA CCGTCCTTTA TCTCTCGGAC ATACGTCGTC TCCTTGCCGT CCCACTTTTG   301 TACGTACACC AGCTTCTCTC CCACCAAGTT CACGACAGAT TTTACGCCTT TTCTTCGATC   361 AGAAGGAAAT TCGTCGAACT CCTCCCCTAA TTTGAACTTT ACTTGAGTGT CAAGGAACGT   421 AGGAGGTCCA TTCTCTATTT TCACTGTCAT TTTGTCTCCG TCCTTCTGGG AGATGTACAA   481 TGTCGGCGTC GTGGCATCCC CACCATCGCT TAATTCCTTT GGGGCTCCGA TAGCTTTCAG   541 GTATTCACCA AAATTATGGC TGTCTGCGAT CTTCCAGGTG CCAACAAATT TCTCGACCAT   601 ACCAGAACCA CCGGATCCCT TATCGTCGTC ATCCTTGTAA TCCATGGTGG CAAGCTTGGG   661 TCTCCCTATA GTGAGTCGTA TTAATTTCGA TAAGCCNGTA AGCAGTGNGT TCTCTAGTTA   721 GCCAGANAGC TCTGCTTATA TAGANCTCCC ACCGNACACG CCTACCGNCC ATTTGCGTCA   781 ATGGGGCGNA NTTGTTACNA CATTTTGNAA AGTCCCNTTG ANTTTGGNGC CNAAACAAAC   841 TCCNATTGAN GTNNNTGGGN NNNNNNNTNN NNNNCCCCNN NNNTCNNNNN GNTNNCNNNN 901 CCNNTNGANG TNCTGNNNAA NNNNNNNNNN //
Primer: SV40pro_F (Pr0110) Region: NeoR Sequence file: RDB15703_A7Lce.seq
>D05018C7_A7Lc_2_SV40pro_F_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNCNNN NNNNTGNNGN NGCTTTTTTG GANGCCTAGG CTTTTGCAAA    61 AAGCTCCCGG GANCTTGTAT ATCCNTTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG   121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG   181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG   241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT   301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA   361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG   421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT   481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA   541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG   601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG   661 CCCGACGGCG AGGNTCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG   721 GANNATGGCC GCTTTTCTGG ATTCATCGAC TGTGNCCGGC TGGGTGTGGC GGANCGCTAT   781 CANGACATAG CGTTGGCTAC CCGTGATATT GCTGAANNNC TTGGCGGCGA ATGGGCTGAN   841 CGCTTCCTCG NGCTTTACNG TATCGCCGCN CCCNANTCNC ANCGCATCGC CTTCNATCGC 901 CNNNNNGNNN ANNNNNNCNG ANCGGNNNTN NNGGGNNNCN NAATNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl