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UnaG/pcDNA3-FLAG (#RDB15703)

Expression vector of eel UnaG, bilirubin-inducible fluorescent protein.

Clone info. Expression vector of eel UnaG, bilirubin-inducible fluorescent protein.
Vector backbone pcDNA3-FLAG (Plasmid)
Selectable markers Amp^r
Gene/insert name Anguilla japonica (Japanese eel) UnaG cDNA
Reference of insert sequence AB763906 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
 
Sequence (full) RDB15703hts01.seq checkAssembled from experimentally sequenced data.
Publication Kumagai, A., Cell 153 (7): 1602-1611(2013). PMID 23768684. [link to RRC of NBRP]
Publication Miyawaki, A., Patent publication US 2016 0009771 A1. [link to RRC of NBRP]
Test sheet RDB15703_B0G8p1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Kumagai, A. et al., Cell 153 (7): 1602-1611 (2013).
Remarks ((Additional form)) Approval Form(FormD)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Kumagai, A. et al., Cell 153 (7): 1602-1611 (2013).
備考 【追加提供依頼書】承諾書(FormD)
提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15703 UnaG/pcDNA3-FLAG DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The UnaG/pcDNA3-FLAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15703).

Reference section:

Kumagai, A., Ando, R., Miyatake, H., Greimel, P., Kobayashi, T., Hirabayashi, Y., Shimogori, T., Miyawaki, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684. [link to RRC of NBRP]
Miyawaki, A., Kumagai, A., Novel polypeptide exhibiting fluorescent properties and use thereof. Patent publication US 2016 0009771 A1. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kumagai, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684. [link to RRC of NBRP]
original Miyawaki, A., Novel polypeptide exhibiting fluorescent properties and use thereof. Patent publication US 2016 0009771 A1. [link to RRC of NBRP]
reference Mu, A., Enhancements of the production of bilirubin and the expression of β-globin by carbon monoxide during erythroid differentiation. FEBS Lett. 590 (10): 1447-1454 (2016). PMID 27087140. [link to Journal]
reference Iwatani, S., Fluorescent protein-based detection of unconjugated bilirubin in newborn serum. Sci. Rep. 6: 28489 (2016). PMID 27324682. [link to Journal]
review Miyawaki, A., Exploiting the cyanobacterial light-harvesting machinery for developing fluorescent probes. Nat. Methods 13 (9): 729-730 (2016). PMID 27575623. [link to Journal]