RDB15703 - UnaG/pcDNA3-FLAG

Catalog number	RDB15703
Resource name	UnaG/pcDNA3-FLAG
Clone info.	Expression vector of eel UnaG, bilirubin-inducible fluorescent protein.
Vector backbone	pcDNA3-FLAG (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Anguilla japonica (Japanese eel) UnaG cDNA
Reference of nucleotide sequence	AB763906 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Kumagai, A., Ando. R,, Miyatake, H., Greimel, P., Kobayashi, T., Hirabayashi, Y., Shimogori, T., Miyawaki, A. (2013) A Bilirubin-Inducible Fluorescent Protein from Eel Muscle. Cell, 153 (7): 1602-1611. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	((Additional form)) Approval Form(FormD)

Distribution information (domestic users)

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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Kumagai, A., Ando. R,, Miyatake, H., Greimel, P., Kobayashi, T., Hirabayashi, Y., Shimogori, T., Miyawaki, A. (2013) A Bilirubin-Inducible Fluorescent Protein from Eel Muscle. Cell, 153 (7): 1602-1611. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	【追加提供依頼書】承諾書(FormD) 提供条件は英文のまま記載してください。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15703	UnaG/pcDNA3-FLAG	DNA solution

original	Kumagai, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684.
original	Miyawaki, A., Novel polypeptide exhibiting fluorescent properties and use thereof. Patent publication US 2016 0009771 A1.

Featured content	Fluorescent protein resources (English text)
Featured content	Fluorescent protein resources (Japanese text)
Featured content	Sensor and Visualization (English text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: CMV_Forward Region: CMV pro,insert,bGHpA Sequence file: RDB15703_A7Lca.seq
>D05018C7_A7Lc_2_CMV-Forward_D02_11_ABI24.ab1 1 AGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC 121 TCACTATAGG GAGACCCAAG CTTGCCACCA TGGATTACAA GGATGACGAC GATAAGGGAT 181 CCGGTGGTTC TGGTATGGTC GAGAAATTTG TTGGCACCTG GAAGATCGCA GACAGCCATA 241 ATTTTGGTGA ATACCTGAAA GCTATCGGAG CCCCAAAGGA ATTAAGCGAT GGTGGGGATG 301 CCACGACGCC GACATTGTAC ATCTCCCAGA AGGACGGAGA CAAAATGACA GTGAAAATAG 361 AGAATGGACC TCCTACGTTC CTTGACACTC AAGTAAAGTT CAAATTAGGG GAGGAGTTCG 421 ACGAATTTCC TTCTGATCGA AGAAAAGGCG TAAAATCTGT CGTGAACTTG GTGGGAGAGA 481 AGCTGGTGTA CGTACAAAAG TGGGACGGCA AGGAGACGAC GTATGTCCGA GAGATAAAGG 541 ACGGTAAACT GGTCGTGACA CTTACGATGG GAGACGTCGT GGCTGTGCGC AGCTACCGGA 601 GGGCGACGGA ATGAGAATTC TGCAGATAGC TAGCTAGCTA TCCATCACAC TGGCGGCCGC 661 TCGAGCATGC ATCTAGAGGG CCCTATTCTA TAGTGTCACC TAAATGCTAG AGCTCGCTGA 721 TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT 781 TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA 841 TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGGTGGGGG CAGGACAGCA 901 AGGGGGGAGG ATTGGGGAAA GACAATAGCA GGCATGCTGG GGGATGCGGG TGGGGCTCTA 961 TGGCTTTCTG AGGCGGGAAG AAACCAGCTG GGGGCTCTAG GGGGTATCCC CCACGCGCCC 1021 TGTAGCCGGC CGCCATTTAA GCCGCGGCGG GCTGTGATTG GTTAACGCCG GCAGGCGATG 1081 ACTGCTACAC TTGGCAGCGA CCCTTAGTCG ACCCCGGACT //
Primer: SV40pro_F Region: SV40 pro_ori,NeoR Sequence file: RDB15703_A7Lcb.seq
>D05018C7_A7Lc_2_SV40pro_F_E02_14_ABI24.ab1 1 CACTGTCCTG GACATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT 781 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 841 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 901 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG AAATGACCGA CCAAGCGACG 961 CCCAACCCTG CCATCACGAG ATTTCGATTC CCACCGCCGC CTTTCTATGA AGGTTGGGCC 1021 TTCGGATCGT TTTCCCGGGA CGCCGGCCTT GTATGGATTC TTCAGGCGCG AGATCTCATG 1081 CTTGGCAAGT TCCATCCGAC CGACCCCAAC GATG //

original	Kumagai, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684.
original	Miyawaki, A., Novel polypeptide exhibiting fluorescent properties and use thereof. Patent publication US 2016 0009771 A1.
reference	Mu, A., Enhancements of the production of bilirubin and the expression of β-globin by carbon monoxide during erythroid differentiation. FEBS Lett. 590 (10): 1447-1454 (2016). PMID 27087140.
reference	Iwatani, S., Fluorescent protein-based detection of unconjugated bilirubin in newborn serum. Sci. Rep. 6: 28489 (2016). PMID 27324682.
review	Miyawaki, A., Exploiting the cyanobacterial light-harvesting machinery for developing fluorescent probes. Nat. Methods 13 (9): 729-730 (2016). PMID 27575623.