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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

UnaG/pcDNA3-FLAG

Expression vector of eel UnaG, bilirubin-inducible fluorescent protein.

Catalog number RDB15703
Resource name UnaG/pcDNA3-FLAG
Clone info. Expression vector of eel UnaG, bilirubin-inducible fluorescent protein.
Vector backbone pcDNA3-FLAG (Plasmid)
Selectable markers Amp^r
Gene/insert name Anguilla japonica (Japanese eel) UnaG cDNA
Reference of nucleotide sequence AB763906 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Kumagai, A., Ando. R,, Miyatake, H., Greimel, P., Kobayashi, T., Hirabayashi, Y., Shimogori, T., Miyawaki, A. (2013) A Bilirubin-Inducible Fluorescent Protein from Eel Muscle. Cell, 153 (7): 1602-1611.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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Material Transfer Agreement (MTA) [Word]
Approval form by depositor [Word]
Remarks ((Additional form)) Approval Form(FormD)
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15703 UnaG/pcDNA3-FLAG DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kumagai, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684.
original Miyawaki, A., Novel polypeptide exhibiting fluorescent properties and use thereof. Patent publication US 2016 0009771 A1.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)
Featured content Sensor and Visualization (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15703_A7Lcp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro,insert,bGHpA
Sequence file: RDB15703_A7Lca.seq check
>D05018C7_A7Lc_2_CMV-Forward_D02_11_ABI24.ab1
    1 AGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTTGCCACCA TGGATTACAA GGATGACGAC GATAAGGGAT
  181 CCGGTGGTTC TGGTATGGTC GAGAAATTTG TTGGCACCTG GAAGATCGCA GACAGCCATA
  241 ATTTTGGTGA ATACCTGAAA GCTATCGGAG CCCCAAAGGA ATTAAGCGAT GGTGGGGATG
  301 CCACGACGCC GACATTGTAC ATCTCCCAGA AGGACGGAGA CAAAATGACA GTGAAAATAG
  361 AGAATGGACC TCCTACGTTC CTTGACACTC AAGTAAAGTT CAAATTAGGG GAGGAGTTCG
  421 ACGAATTTCC TTCTGATCGA AGAAAAGGCG TAAAATCTGT CGTGAACTTG GTGGGAGAGA
  481 AGCTGGTGTA CGTACAAAAG TGGGACGGCA AGGAGACGAC GTATGTCCGA GAGATAAAGG
  541 ACGGTAAACT GGTCGTGACA CTTACGATGG GAGACGTCGT GGCTGTGCGC AGCTACCGGA
  601 GGGCGACGGA ATGAGAATTC TGCAGATAGC TAGCTAGCTA TCCATCACAC TGGCGGCCGC
  661 TCGAGCATGC ATCTAGAGGG CCCTATTCTA TAGTGTCACC TAAATGCTAG AGCTCGCTGA
  721 TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT
  781 TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA
  841 TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGGTGGGGG CAGGACAGCA
  901 AGGGGGGAGG ATTGGGGAAA GACAATAGCA GGCATGCTGG GGGATGCGGG TGGGGCTCTA
  961 TGGCTTTCTG AGGCGGGAAG AAACCAGCTG GGGGCTCTAG GGGGTATCCC CCACGCGCCC
 1021 TGTAGCCGGC CGCCATTTAA GCCGCGGCGG GCTGTGATTG GTTAACGCCG GCAGGCGATG
1081 ACTGCTACAC TTGGCAGCGA CCCTTAGTCG ACCCCGGACT
//
Primer: SV40pro_F
Region: SV40 pro_ori,NeoR
Sequence file: RDB15703_A7Lcb.seq check
>D05018C7_A7Lc_2_SV40pro_F_E02_14_ABI24.ab1
    1 CACTGTCCTG GACATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG
  121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG
  661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  781 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  841 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  901 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG AAATGACCGA CCAAGCGACG
  961 CCCAACCCTG CCATCACGAG ATTTCGATTC CCACCGCCGC CTTTCTATGA AGGTTGGGCC
 1021 TTCGGATCGT TTTCCCGGGA CGCCGGCCTT GTATGGATTC TTCAGGCGCG AGATCTCATG
1081 CTTGGCAAGT TCCATCCGAC CGACCCCAAC GATG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kumagai, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684.
original Miyawaki, A., Novel polypeptide exhibiting fluorescent properties and use thereof. Patent publication US 2016 0009771 A1.
reference Mu, A., Enhancements of the production of bilirubin and the expression of β-globin by carbon monoxide during erythroid differentiation. FEBS Lett. 590 (10): 1447-1454 (2016). PMID 27087140.
reference Iwatani, S., Fluorescent protein-based detection of unconjugated bilirubin in newborn serum. Sci. Rep. 6: 28489 (2016). PMID 27324682.
review Miyawaki, A., Exploiting the cyanobacterial light-harvesting machinery for developing fluorescent probes. Nat. Methods 13 (9): 729-730 (2016). PMID 27575623.

2020.10.02

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