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UnaG/pcDNA3-FLAG (#RDB15703)

Expression vector of eel UnaG, bilirubin-inducible fluorescent protein.

Clone info. Expression vector of eel UnaG, bilirubin-inducible fluorescent protein.
Vector backbone pcDNA3-FLAG (Plasmid)
Selectable markers Amp^r
Gene/insert name Anguilla japonica (Japanese eel) UnaG cDNA
Reference of insert sequence AB763906 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

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Terms and conditions for distribution 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Kumagai, A. et al., Cell 153 (7): 1602-1611 (2013).
Remarks ((Additional form)) Approval Form(FormD)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Kumagai, A. et al., Cell 153 (7): 1602-1611 (2013).
備考 【追加提供依頼書】承諾書(FormD)
提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15703 UnaG/pcDNA3-FLAG DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The UnaG/pcDNA3-FLAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15703).

Reference section:

Kumagai, A., Ando, R., Miyatake, H., Greimel, P., Kobayashi, T., Hirabayashi, Y., Shimogori, T., Miyawaki, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15703_A7Lcp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert 5'(FLAG,UnaG 5')
Sequence file: RDB15703_A7Lcc.seq check
>D05018C7_A7Lc_2_CMV-Forward_D02_11_ABI24.ab1
    1 NNNNNNNNNN NCCNTNNNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTTGCCACCA TGGATTACAA GGATGACGAC GATAAGGGAT
  181 CCGGTGGTTC TGGTATGGTC GAGAAATTTG TTGGCACCTG GAAGATCGCA GACAGCCATA
  241 ATTTTGGTGA ATACCTGAAA GCTATCGGAG CCCCAAAGGA ATTAAGCGAT GGTGGGGATG
  301 CCACGACGCC GACATTGTAC ATCTCCCAGA AGGACGGAGA CAAAATGACA GTGAAAATAG
  361 AGAATGGACC TCCTACGTTC CTTGACACTC AAGTAAAGTT CAAATTAGGG GAGGAGTTCG
  421 ACGAATTTCC TTCTGATCGA AGAAAAGGCG TAAAATCTGT CGTGAACTTG GTGGGAGAGA
  481 AGCTGGTGTA CGTACAAAAG TGGGACGGCA AGGAGACGAC GTATGTCCGA GAGATAAAGG
  541 ACGGTAAACT GGTCGTGACA CTTACGATGG GAGACGTCGT GGCTGTGCGC AGCTACCGGA
  601 NGGCGACGGA ATGAGAATTC TGCAGATAGC TAGCTAGCTA TCCATCACAC TGGCGGCCGC
  661 TCGAGCATGC ATCTAGAGGG CCCTATTCTA TAGTGTCACC TAAATGCTAG AGCTCGCTGA
  721 TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC NCCCGTGCCT
  781 TCCTTGACCC TGGAANGTGC CACTCNNACT GTCCTTTCCT AATAAAATGA NGAAATTGCA
  841 TCGCATNNNC TGANTANGNG TCATTCTATT CTGGGGGNNN GGGGNGGGGN NANGNNANCN
  901 ANGGGGGANN NNTGGGGNAN NANNNNNNCN NNNNTGCNGG GGNNNGCNGN NNGGNNNNNN
961 NNNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGH pA,insert(UnaG,FLAG)
Sequence file: RDB15703_A7Lcd.seq check
>15703_15703_B3BeF_BGH_rev2_H01_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GGNNGNNGGG CNNACNACAG ATGGCTGGCA ACTAGAAGGC
   61 ACAGTCGAGG CTGATCAGCG AGCTCTAGCA TTTAGGTGAC ACTATAGAAT AGGGCCCTCT
  121 AGATGCATGC TCGAGCGGCC GCCAGTGTGA TGGATAGCTA GCTAGCTATC TGCAGAATTC
  181 TCATTCCGTC GCCCTCCGGT AGCTGCGCAC AGCCACGACG TCTCCCATCG TAAGTGTCAC
  241 GACCAGTTTA CCGTCCTTTA TCTCTCGGAC ATACGTCGTC TCCTTGCCGT CCCACTTTTG
  301 TACGTACACC AGCTTCTCTC CCACCAAGTT CACGACAGAT TTTACGCCTT TTCTTCGATC
  361 AGAAGGAAAT TCGTCGAACT CCTCCCCTAA TTTGAACTTT ACTTGAGTGT CAAGGAACGT
  421 AGGAGGTCCA TTCTCTATTT TCACTGTCAT TTTGTCTCCG TCCTTCTGGG AGATGTACAA
  481 TGTCGGCGTC GTGGCATCCC CACCATCGCT TAATTCCTTT GGGGCTCCGA TAGCTTTCAG
  541 GTATTCACCA AAATTATGGC TGTCTGCGAT CTTCCAGGTG CCAACAAATT TCTCGACCAT
  601 ACCAGAACCA CCGGATCCCT TATCGTCGTC ATCCTTGTAA TCCATGGTGG CAAGCTTGGG
  661 TCTCCCTATA GTGAGTCGTA TTAATTTCGA TAAGCCNGTA AGCAGTGNGT TCTCTAGTTA
  721 GCCAGANAGC TCTGCTTATA TAGANCTCCC ACCGNACACG CCTACCGNCC ATTTGCGTCA
  781 ATGGGGCGNA NTTGTTACNA CATTTTGNAA AGTCCCNTTG ANTTTGGNGC CNAAACAAAC
  841 TCCNATTGAN GTNNNTGGGN NNNNNNNTNN NNNNCCCCNN NNNTCNNNNN GNTNNCNNNN
901 CCNNTNGANG TNCTGNNNAA NNNNNNNNNN
//
Primer: SV40pro_F (Pr0110)
Region: NeoR
Sequence file: RDB15703_A7Lce.seq check
>D05018C7_A7Lc_2_SV40pro_F_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNNN NNNNTGNNGN NGCTTTTTTG GANGCCTAGG CTTTTGCAAA
   61 AAGCTCCCGG GANCTTGTAT ATCCNTTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG
  121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG
  661 CCCGACGGCG AGGNTCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  721 GANNATGGCC GCTTTTCTGG ATTCATCGAC TGTGNCCGGC TGGGTGTGGC GGANCGCTAT
  781 CANGACATAG CGTTGGCTAC CCGTGATATT GCTGAANNNC TTGGCGGCGA ATGGGCTGAN
  841 CGCTTCCTCG NGCTTTACNG TATCGCCGCN CCCNANTCNC ANCGCATCGC CTTCNATCGC
901 CNNNNNGNNN ANNNNNNCNG ANCGGNNNTN NNGGGNNNCN NAATNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kumagai, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684. [link to RRC of NBRP]
reference Mu, A., Enhancements of the production of bilirubin and the expression of β-globin by carbon monoxide during erythroid differentiation. FEBS Lett. 590 (10): 1447-1454 (2016). PMID 27087140.
reference Iwatani, S., Fluorescent protein-based detection of unconjugated bilirubin in newborn serum. Sci. Rep. 6: 28489 (2016). PMID 27324682.
review Miyawaki, A., Exploiting the cyanobacterial light-harvesting machinery for developing fluorescent probes. Nat. Methods 13 (9): 729-730 (2016). PMID 27575623.