Please review the QC test results indicated by icon below as well as clone information before placing your order.

tdStayGold(c4)/pBS Coupler

Fluorescent protein tdStayGold for fusion to the N-terminus of target molecule

Sequence (full)	RDB19610zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

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提供条件	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19610_B2Eap1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBAC_lac_F (Pr0544) Region: T7 pro,insert 5' Sequence file: RDB19610_B2Eaa.seq
>D08836A6_B2Ea_1_pBAC_lac_F_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNCNNNNNNA ANACGACTCN CTATAGGGCG AATTGGAGCT    61 CCACCGCGGT GGCGGCCGCA GCCACCATGG TGTCTACAGG CGAGGAGCTG TTTACCGGCG   121 TGGTGCCCTT CAAGTTCCAG CTGAAGGGCA CCATCAACGG CAAGAGCTTC ACCGTGGAAG   181 GCGAGGGCGA GGGCAATAGC CACGAGGGCA GCCACAAAGG CAAGTACGTG TGCACCAGCG   241 GCAAACTGCC AATGTCTTGG GCCGCCCTGG GAACTAGCTT CGGCTATGGC ATGAAGTACT   301 ACACCAAGTA CCCCAGCGGC CTGAAGAACT GGTTCCACGA GGTGATGCCC GAGGGCTTCA   361 CCTACGACAG ACACATCCAG TACAAGGGCG ACGGCAGCAT CCACGCCAAG CACCAGCACT   421 TCATGAAGAA CGGCACCTAC CACAACATCG TGGAGTTCAC CGGCCAGGAC TTCAAGGAGA   481 ACAGCCCCGT GCTGACCGGC GACATGAACG TGAGCCTGCC CAACGAGGTG CAGCACATCC   541 CCAGAGATGA CGGCGTGGAG TGCCCAGTGA CCCTGCTGTA CCCTCTGCTG AGCGACAAGA   601 GCAAGTGCGT GGAGGCCCAC CAGAACACCA TCTGCAAGCC CCTGCACAAT CAGCCAGCCC   661 CCGATGTGCC ATACCACTGG ATCAGAAAGC AGTACACCCA GAGCAAGGAC GACACCGAGG   721 AGAGAGACCA CATCTNNNNN AGCGAGACCC TGGANGCCCA CCTGCCATGG CACGAGCCTT   781 CTGCTTCTGC CGTGNAATTC AGTGCTGGTG GTTCTGCTGG ANGAAGCGCC GGANGTTCCG   841 CTNGGGGAAG CNNANGCGGC TCCGGCAGCG CTGGCGGGAN TGCCGGGGGC AGTACCTCNN   901 CNNAGGAAGT GCAGGGGGAN NCGCTNGTGG CAGTGCGGGG GGNNNCGCNN NCNGNANNNN   961 NTNNCNCNNG NNGATCTGCC NGTGGAANCN CATCTGCNNG GGGGTNNTGN NNNNNGGTNN 1021 NCNNNNNNGG NNNCNNNNNN NNNN //
Primer: reverse-2 (Pr0100) Region: T3 pro,(GGGGS)x3,insert 3' Sequence file: RDB19610_B2Eab.seq
>D08836A6_B2Ea_1_reverse-2_F05_17_ABI24.ab1     1 NNNNNNNNNN NNNNCNNNCG CNNNNNAACC CTCNCTAAAG GGAACAAAAG CTGGGTACCG    61 GGCCCCCCCT CGAGGTCGAC GGTATCGATA AGCTTGATGG CGATCCGCCA CCGCCAGAGC   121 CACCTCCGCC TGAACCGCCT CCACCATCGA ATTCCTGCAG CCCGGGGGAT CCTACTGCCG   181 ATGCTGAAGG CTCATGCCAA GGTAAGTGTG CTTCGAGAGT CTCTGATTGA CAAATATGAT   241 CACGTTCCTC GGTATCATCT TTGCTTTGTG TGTATTGTTT ACGAATCCAG TGATATGGGA   301 CATCAGGTGC TGGTTGATTA TGAAGAGGCT TGCAGATTGT ATTTTGGTGA GCCTCAACGC   361 ATTTTGATTT ATCCGATAAT AAAGGATAAA GCAAGGTCAC TGGGCATTCT ACTCCATCAT   421 CTCTGGGTAT ATGTTGAACT TCATTCGGTA ATGAGACATT CATATCTCCA GTTAAGACTG   481 GACTATTTTC TTTAAAATCC TGACCAGTAA ATTCAACAAT GTTGTGATAA GTCCCATTTT   541 TCATAAAGTG TTGGTGTTTT GCATGGATAC TCCCATCGCC TTTATATTGA ATATGACGAT   601 CGTAGGTAAA GCCTTCAGGC ATTACTTCAT GAAACCAGTT CTTCAGTCCA CTAGGATATT   661 TGGTATAATA TTTCATTCCA TAACCAAAGG ATGTTCCAAG TGCTGCCCAT GACATCGGTA   721 GTTTTCCACT TGTACAAACA TATTTTCCTT TATGAGAACC TTCATGTGAA TTTCCTTCAC   781 CTTCGCCTTC AACGGTAAAC GATTTGCCAT TGATGGTTCC TTTAAGTTGA AATNNNNNGG   841 CACCACCCCG GTGAACAGCT CCTCNCNNGT ACTCACCATA AGCTTCCCAC CGGCGCTANC   901 GCCGGCNCTC CCNNCGGCGG ANNCGCCAGC ANANCCACCN GNANANGTGN ANCNNCCANC   961 GNTACCNNCT GNNCTTCCAN ANCCCCCGGN ANANNCNNCT GNNCTNCNNN NANCGGANCN 1021 NNNNGNNNTA NCCNNNNNAN NNGNGNTTNC NNCNNNNNNN NNN //
Primer: hStayGold_F1 (Pr1213) Region: insert mid Sequence file: RDB19610_B2Eac.seq
>D08836A6_B2Ea_1_hStayGold_F1_D05_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNTGCC NNACGAGGTG CAGCACATCC CCNGAGATGA    61 CGGCGTGGAG TGCCCAGTGN NCCTGCTGTA CCCTCTGCTG AGCGACAAGA GCAAGTGCGT   121 GGAGGCCCAC CAGAACACCA TCTGCAAGCC CCTGCACAAT CAGCCAGCCC CCGATGTGCC   181 ATACCACTGG ATCAGAAAGC AGTACACCCA GAGCAAGGAC GACACCGAGG AGAGAGACCA   241 CATCTGCCAG AGCGAGACCC TGGAGGCCCA CCTGCCATGG CACGAGCCTT CTGCTTCTGC   301 CGTGGAATTC AGTGCTGGTG GTTCTGCTGG AGGAAGCGCC GGAGGTTCCG CTGGGGGAAG   361 CGCAGGCGGC TCCGGCAGCG CTGGCGGGAG TGCCGGGGGC AGTACCTCCG CAGGAGGAAG   421 TGCAGGGGGA AGCGCTGGTG GCAGTGCGGG GGGTTCCGCA GGCGGTAGCG GTTCCGCCGG   481 TGGATCTGCC GGTGGAAGCA CATCTGCTGG GGGTAGTGCA GGAGGGTCCG CTGGTGGGAG   541 CGCAGGTGGA TCTGCCGGGG GGTCTGGAAG TGCAGGTGGT AGCGCTGGAG GGTCCACATC   601 TGCAGGTGGG TCTGCTGGCG GTTCCGCCGG AGGGAGTGCC GGCGGTAGCG CCGGTGGGAA   661 GCTTATGGTG AGTACAGGGG AGGAGCTGTT CACCGGGGTG GTGCCATTTA AATTTCAACT   721 TAAAGGAACC ATCAATGGCA AATCGTTTAC CGTTGAAGGC GAANGTGAAG GAANTTCACA   781 TGAANGTTCT CATAAAGGAA AATATGTTTG TACAAGTGGA AAACTACCGA TGTCATGGGC   841 AGCACTTGNA ACATCCTTTG GTTATGGAAT GAAATATTAT ACCNNATATC CTANTGGANT   901 GAAGAANTGG TTTCATGAAG TAATGNCTGA ANGNTTTANC TACNATCGTC ATANNCNATA   961 TAAAGGNNAT GGGANTATCC NTGCNAAANA CCNNCNCTTT NTGAAAAATG GGNNTNATCN 1021 NNNNNTTNNT NGNNNTTNCN NNNNNNNANT TNNAANNAAA NNNNNNNNNN //
Primer: EV_linker_F1 (Pr1214) Region: insert mid Sequence file: RDB19610_B2Ead.seq
>D08836A6_B2Ea_1_EV_linker_F1_E05_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGNN GGNNCNCNNG TGGNNCTGCC GGGGGGTCTG GAAGTGCAGG    61 TGGTAGCGCT GGAGGGTCCA CATCTGCAGG TGGGTCTGCT GGCGGTTCCG CCGGAGGGAG   121 TGCCGGCGGT AGCGCCGGTG GGAAGCTTAT GGTGAGTACA GGGGAGGAGC TGTTCACCGG   181 GGTGGTGCCA TTTAAATTTC AACTTAAAGG AACCATCAAT GGCAAATCGT TTACCGTTGA   241 AGGCGAAGGT GAAGGAAATT CACATGAAGG TTCTCATAAA GGAAAATATG TTTGTACAAG   301 TGGAAAACTA CCGATGTCAT GGGCAGCACT TGGAACATCC TTTGGTTATG GAATGAAATA   361 TTATACCAAA TATCCTAGTG GACTGAAGAA CTGGTTTCAT GAAGTAATGC CTGAAGGCTT   421 TACCTACGAT CGTCATATTC AATATAAAGG CGATGGGAGT ATCCATGCAA AACACCAACA   481 CTTTATGAAA AATGGGACTT ATCACAACAT TGTTGAATTT ACTGGTCAGG ATTTTAAAGA   541 AAATAGTCCA GTCTTAACTG GAGATATGAA TGTCTCATTA CCGAATGAAG TTCAACATAT   601 ACCCAGAGAT GATGGAGTAG AATGCCCAGT GACCTTGCTT TATCCTTTAT TATCGGATAA   661 ATCAAAATGC GTTGAGGCTC ACCAAAATAC AATCTGCAAG CCTCTTCATA ATCAACCAGC   721 ACCTGATGTC CCATATCACT GGATTCGTAA ACAATACACA CAAAGCAAAG ATGATACCGA   781 GGAACGTGAT CATATTTGTC AATCAGAGAC TCTCGAANCA CACTTACCTT GGCATGAGCC   841 TTCNGCATCG GCAGTANGNN CCCCCGGGCT GCANGAATTC GATGGTGGAN GCGGNTCNNN   901 CGGANGTGNN TCTGGNGGNG GCGGNNCGCC ATCAAGCTNA TCNATACCGT CNANCNCNAG   961 GGGGGGCCCN GNNNCCANCT TTTGNTCCNN TTANTGANGG NNNNNNGNNN NCTTNNNNNN 1021 NNCNTNNNNA TANCTNNTNN CNNNNNNNNA NNNNNNNNNN //
Primer: pBR322-ori-R (Pr1218) Region: pBR322 ori 3',AmpR Sequence file: RDB19610_B2Eae.seq
>D08836A6_B2Ea_1_pBR322-ori-R_C10_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN GGNAGCTCTT GNNCCGGNNN ANNNACCACC GCTGGTAGCG    61 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC   121 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT   181 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT   241 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA   301 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG   361 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC   421 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG   481 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC   541 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA   601 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC   661 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC   721 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCNC   781 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGNGNNTANT   841 CAACCAAGTC ATTCTGANAN TAGTGTATGC NGCGNNCGAN TTGCTCTTGC CCGGCGTCNA   901 NTNNNGGATA ATACNNCGCN NCATANCANA ANTTTNAAAG TGNTCATNAT TGNNAAANGT   961 TCTTNGGGNN NAAAACTCTC NNNNANCTTN NCNNNTGTTG ANANNCNGTT CNANGTNNCC 1021 CANTNNNGNN NCCNNNNNGA TNNNNNNCNT NTTTNNNTTN NNNNNNNNN //
Primer: f1ori_F (Pr0280) Region: f1 ori,AmpR pro,AmpR Sequence file: RDB19610_B2Eaf.seq
>D08836A6_B2Ea_1_f1ori_F_B10_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGTAGCGG TCNCGCTGCG CGTAACCACC ACACCCGCCG    61 CGCTTAATGC GCCGCTACAG GGCGCGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA   121 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC   181 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG   241 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC   301 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG   361 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA   421 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC   481 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG   541 AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA   601 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG   661 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA   721 ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT   781 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT   841 GGANGGANGC GGANAAAGTT GCAGGANCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGN   901 TNATTGNTGA NAAATCTGGA NCCGGNGANC GNGGGTCTCG CGGTATCNTT GNNGCNNNGG 961 GGNNNGANGG NAAGCCCNNC CGTANNGNAN NNATCTACNC NANNGGGNNN NNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl