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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tFucci(SA)5/pCS2

New color variant of Fucci: Fucci(SA)5.

Catalog number RDB18220
Resource name tFucci(SA)5/pCS2
Clone info. New color variant of Fucci: Fucci(SA)5.
Comment The accession numbers in the DDBJ/EMBL/GenBank database is LC334437.
Vector backbone pCS2 (plasmid)
Size of vector backbone 6.2 kb
Selectable markers Ampicillin
Gene/insert name Montipora monasteriata RFP cDNA Ricordea sp. GFP cDNA human CDT1 cDNA human Geminin cDNA
Reference of insert sequence LC334437 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi | Sakaue-Sawano, Asako | Ando, Ryoko |
Other clones in our bank

External Database
Montipora monasteriata RFP
    Ricordea sp. GFP
      human CDT1 human Geminin

        Reference sequence
          
         
        Sequence (full) RDB18220zzt01.seq compiled by us

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
        4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A. (2020) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv doi: https://doi.org/10.1101/2020.03.30.015156.
        5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
        Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        Approval form [Word] by Dr. Atsushi Miyawaki
        Remarks
        Please obtain written approval of Dr. Atsushi Miyawaki.
        提供案内 (日本国内) [open/close]

        提供条件 1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
        4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A. (2020) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv doi: https://doi.org/10.1101/2020.03.30.015156.
        5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        宮脇敦史博士による提供承諾書 [Word]
        備考 提供条件は英文のまま記載してください。
        宮脇敦史博士の書面による提供承諾が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB18220 tFucci(SA)5/pCS2 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Original reference

        original Ando, R., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Fucci-related materials (en) (English text)
        Featured content Fucci-related materials (ja) (Japanese text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB18220_B0Fbp1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: pCMV-IE94_F
        Region: CMV IE94 pro, SP6 pro, insert 5'
        Sequence file: RDB18220_B0Fba.seq check
        >D07168A3_B0Fb_1_pCMV-TE94_F_D02_11_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNGGGAN GNCTATATNA GCAATGCTCG TTTAGGGAAC
           61 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG
          121 CTACTTGTTC TTTTTGCAGG ATCCGCCACC ATGGAGAACG TCAGGAGAAA GACAGGCATT
          181 CAGACGGAAA TGAAAACGAA GCTCCACATG GATGGTATGG TGAATGGACA CAGCTTTGAG
          241 ATCAAAGGGG AGGGGAAGGG AAGCCCGTAC GAGGGGGTGC AGACAATGAA GCTGAAGGTT
          301 ACTAAGGGCG CCCCACTTCC CTTCTCTATA GACATTCTTT TGCCTCAGTG CATGTATGGC
          361 AGCAAACCTT TCATTAAATA TCCTGAGAAC ATCCCCGATT ATTTGAAACT CTCATTTCCA
          421 GAGGGTATCA CTTGGGAGAG GACAATGACA TTCGAGGACG GCGCAGTATG CGATGTATCT
          481 AATGATTCCA GACTGGTCGG CAATTGCTTT ATCTACACTG TGAAGTTTCA GGGAGTCAAT
          541 TTTCCACTTG ATGGCCCCGT TATGCAAAAG AAGACCCGAG GCTGGGAACC CTCCACTGAA
          601 GTGCTGTACG AATGTGATGG GTGGATGCGC GGGTTGGTGG ACATAGCTCT GAAACTGGAA
          661 AACGGTGGAC ATTATATGTG TAATTTTAAG ACCACCTACA AATCCAAGAA AGGTCTTGAG
          721 GTGCCACCTT ACCATTTCGT GGATCATAAA CTCGANCTGC TCAGTCATAA CACCGACGGA
          781 NCCACCTTTG ANGANTTCGA ACAAGGTGAA ATCGCTCACG CGCACTTGAN NNAACTNGCC
          841 GGATATNCAT CACACTGGCG GNNGCTCNAG CCCAGCCCNN NCAGGNCCGC ACTNNNCGCC
          901 NNNGNCNNCN CTACCANNGG NAGCNNNAAG CGCGNCNGCC CNNCCGNNGN CCCCCNNNNN
        961 NNANNNNNNN NNN
        //
        Primer: pGCAP_R
        Region: T7 pro, insert 3'
        Sequence file: RDB18220_B0Fbb.seq check
        >D07168A3_B0Fb_1_pGCAP_R_E02_14_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNGGNNNNN CGTAATACGA CTCNCTATAG GGCTGCAGAA
           61 TCTAGATTAC AGCGCCTTTC TCCGTTTTTC TGCCACTTCC TTCCAATACT GAGAGGATGG
          121 ATTTTCTTTA ATCATAAGAT CAAATGACTC CTGGGTGACT CCTCCAAGAT TTTTATTTTC
          181 ACTAGATTCT GGGACAATAA CCCCAGGGCT GGAAGTTGTA GATGTTAAGT GGTCATTCCG
          241 ATGTTTCCTT TTGGACAAGC CTGCGGACAG CTCATTTTCT CTTCCAACAA GAGATCCAGA
          301 TGCAGAAGGC TGAATCATCT TCAGAGTTCT TCTTGGGACA GAACTATTCT TTATATTCTC
          361 TTTGATTTCT TCTTGTTTCT GCTTCATACT GGGATTCATC TCGAGCGGCC GCCAGTGTGA
          421 TGGATATCCC TTATTCAGGT TCTTTTCAGG CAGCAGGCTG TACCTGGCCA CAGCCCCTGC
          481 GTAGAGCTTG ACAGGGCGTC CTTCGGGCTT CTCCAGGATC TCGATCCGGT GGTCCACGAA
          541 GTGGTAATCT GGCATCTTCT CCACCTTATT TTTGGGAATA TAAGAAGTGT TGAAATCGAC
          601 TCTGAGGTGA CCACCATCCT TCAACAGCAG GGCCATATTC ACGTCGCCCT TCAGCACTCC
          661 ATCGTTTTTA TCGTCCTTGT ACATCACCTC GGTGGAAGGT TCCCATTTGA CTGTTTCCTT
          721 TTGCATGACT GGGCCGTGGG GGGGGAACTT TCCGTTGAAT TGTATTTTAT AATGGAAGCA
          781 GTCCCCTTGA ACGCTGATAT CGNACTCAAC GGTTGAGACN CCGCCGTCTT CATAGGTCAT
          841 TGTTCNTTTN CATGNNNATC CCCCGCCACT GAANGNCTGT TTGAANAAGT CTGGNATGTT
          901 ACCGGGGNAC NNGGNNAACG CCNNATTGNC GTANTGAANN GNTGGGGNNA GNANNTCGAA
        961 NCTGANNNNN NNNGTCTTTN CNNNCNNNNN CNNNCNNNNN NNNNN
        //
        Primer: Azalea5B_F
        Region: insert mid
        Sequence file: RDB18220_B0Fbc.seq check
        >D07168A3_B0Fb_1_Azalea5B_F_F02_17_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGGNCTTG AGGTGCCACC TTACCNTTTC
           61 GTGGATCATA AACTCGACCT GCTCAGTCAT AACACCGACG GAGCCACCTT TGAGGAGTTC
          121 GAACAAGGTG AAATCGCTCA CGCGCACTTG AGTAAACTGG CCGGATATCC ATCACACTGG
          181 CGGCCGCTCG AGCCCAGCCC CGCCAGGCCC GCACTCCGCG CCCCGGCCTC CGCTACCAGT
          241 GGCAGCCGCA AGCGCGCCCG CCCGCCCGCC GCCCCCGGAC GCGACCAGGC CAGGCCACCG
          301 GCCCGCAGGA GACTGCGGCT GTCGGTGGAC GAGGTTTCCA GCCCCAGTAC CCCCGAGGCC
          361 CCAGACATCC CAGCCTGCCC TTCTCCGGGC CAGAAGATAA AGAAATCCAC CCCGGCAGCA
          421 GGTCAGCCGC CCCACCTGAC ATCCGCGCAG GACCAGGACA CCATCACCGG TCGCGCCAAG
          481 CGCGGCAGCG GCGCCACCAA CTTCAGCCTG CTGAAGCAGG CCGGCGACGT GGAGGAGAAC
          541 CCCGGCCCCC GCGAATTCAT GAGTAAACTC CAGAAAGGTG TGGAGAAAGA GATGAAGATT
          601 AAGCTCACTA TGAACTGCAC CGTGAACGAA CGGAACTTTG TGATCACAGG GCAGGGGGCT
          661 GGGGAACCTT ATGATGGAAN TCAGACACTT TACCTGACAG TGGAGGGCGG AAAGACACTG
          721 AACTTCAGCT TCGATGTACT GACCCCAGCC TTTCAGTACG GCAATAGGGC GTTTACCAAG
          781 TACCCCGGTA ACATACCAGA CTTCTTCAAA CAGACCTTCA GTGGCGGGGG ANACACATGG
          841 AAAAAGANNA ATGACNTATG AAGACGGNNG GCGNNTCNNC CGTTGANTCC GATATCANCG
          901 TTNCAAGGGG ANTGCTTCCN NTNANANNTA NNATTCNNCG GAAANTNNCC NCCCCNNNGG
          961 CCCANNCNNN CAAAANGAAA NNGNCNAATG GGNAANCNTT NNCNNNNNNG NNNTGNNNNA
        1021 NNNNNNNNNA NNNNNNNNNN
        //
        Primer: tFucci_f1
        Region: insert mid
        Sequence file: RDB18220_B0Fbd.seq check
        >D07168A3_B0Fb_1_tFucci_f1_G02_20_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNCN NNNNTTCTCC NGGCNNNNAA GNNAANNGNA ANTCCACCCC
           61 GGCAGCAGGT CAGCCGCCCC ACCTGACATC CGCGCNNGAC CAGGACACCA TCACCGGTCG
          121 CGCCAAGCGC GGCAGCGGCG CCACCAACTT CAGCCTGCTG AAGCAGGCCG GCGACGTGGA
          181 GGAGAACCCC GGCCCCCGCG AATTCATGAG TAAACTCCAG AAAGGTGTGG AGAAAGAGAT
          241 GAAGATTAAG CTCACTATGA ACTGCACCGT GAACGAACGG AACTTTGTGA TCACAGGGCA
          301 GGGGGCTGGG GAACCTTATG ATGGAACTCA GACACTTTAC CTGACAGTGG AGGGCGGAAA
          361 GACACTGAAC TTCAGCTTCG ATGTACTGAC CCCAGCCTTT CAGTACGGCA ATAGGGCGTT
          421 TACCAAGTAC CCCGGTAACA TACCAGACTT CTTCAAACAG ACCTTCAGTG GCGGGGGATA
          481 CACATGGAAA AGAACAATGA CCTATGAAGA CGGCGGCGTC TCAACCGTTG AGTCCGATAT
          541 CAGCGTTCAA GGGGACTGCT TCCATTATAA AATACAATTC AACGGAAAGT TCCCCCCCCA
          601 CGGCCCAGTC ATGCAAAAGG AAACAGTCAA ATGGGAACCT TCCACCGAGG TGATGTACAA
          661 GGACGATAAA AACGATGGAG TGCTGAAGGG CGACGTGAAT ATGGCCCTGC TGTTGAAGGA
          721 TGGTGGTCAC CTCAGAGTCG ATTTCAACAC TTCTTATATT CCNAAAAATA AGGTGGANAA
          781 GATGCCAGAT TANCACTTCN TGGANCANCG GATCGANATC CTGGANAANC CCGAAGGANG
          841 CCCNGTCNNG CTCTACGCNN GGGCTGTGGN CAGGNTACNN NCNGCNGNCT GAAAANAANN
          901 TGAATAAGGN ATNTCCNTCA CACNGGNCNG NCGCNCGAGA TGNATCCNNG TATGANNNNG
          961 AAANNAGAAN AAATCNAAGA NAANNATNAA NNANNGTTNN GNNNCCNNNN AANAANNNNN
        1021 NNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Ando, R., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

        2022.08.12

        GNP_filter3_RDBDEP_html_220812.pl