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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tFucci(SA)5/pCS2

New color variant of Fucci: Fucci(SA)5.

Catalog number RDB18220
Resource name tFucci(SA)5/pCS2
Clone info. New color variant of Fucci: Fucci(SA)5.
The accession numbers in the DDBJ/EMBL/GenBank database is LC334437.
Vector backbone pCS2 (plasmid)
Size of vector backbone 6.2 kb
Selectable markers Ampicillin
Gene/insert name Montipora monasteriata RFP cDNA Ricordea sp. GFP cDNA human CDT1 cDNA human Geminin cDNA
Reference of nucleotide sequence LC334437 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi | Sakaue-Sawano, Asako | Ando, Ryoko |
 
Other clones in our bank human CDT1 (NCBI Gene 81620) | human GMNN (NCBI Gene 51053) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A. (2020) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv doi: https://doi.org/10.1101/2020.03.30.015156.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18220 tFucci(SA)5/pCS2 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Ando, R., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18220_B0Fbp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F
Region: CMV IE94 pro, SP6 pro, insert 5'
Sequence file: RDB18220_B0Fba.seq check
>D07168A3_B0Fb_1_pCMV-TE94_F_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGGGAN GNCTATATNA GCAATGCTCG TTTAGGGAAC
   61 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG
  121 CTACTTGTTC TTTTTGCAGG ATCCGCCACC ATGGAGAACG TCAGGAGAAA GACAGGCATT
  181 CAGACGGAAA TGAAAACGAA GCTCCACATG GATGGTATGG TGAATGGACA CAGCTTTGAG
  241 ATCAAAGGGG AGGGGAAGGG AAGCCCGTAC GAGGGGGTGC AGACAATGAA GCTGAAGGTT
  301 ACTAAGGGCG CCCCACTTCC CTTCTCTATA GACATTCTTT TGCCTCAGTG CATGTATGGC
  361 AGCAAACCTT TCATTAAATA TCCTGAGAAC ATCCCCGATT ATTTGAAACT CTCATTTCCA
  421 GAGGGTATCA CTTGGGAGAG GACAATGACA TTCGAGGACG GCGCAGTATG CGATGTATCT
  481 AATGATTCCA GACTGGTCGG CAATTGCTTT ATCTACACTG TGAAGTTTCA GGGAGTCAAT
  541 TTTCCACTTG ATGGCCCCGT TATGCAAAAG AAGACCCGAG GCTGGGAACC CTCCACTGAA
  601 GTGCTGTACG AATGTGATGG GTGGATGCGC GGGTTGGTGG ACATAGCTCT GAAACTGGAA
  661 AACGGTGGAC ATTATATGTG TAATTTTAAG ACCACCTACA AATCCAAGAA AGGTCTTGAG
  721 GTGCCACCTT ACCATTTCGT GGATCATAAA CTCGANCTGC TCAGTCATAA CACCGACGGA
  781 NCCACCTTTG ANGANTTCGA ACAAGGTGAA ATCGCTCACG CGCACTTGAN NNAACTNGCC
  841 GGATATNCAT CACACTGGCG GNNGCTCNAG CCCAGCCCNN NCAGGNCCGC ACTNNNCGCC
  901 NNNGNCNNCN CTACCANNGG NAGCNNNAAG CGCGNCNGCC CNNCCGNNGN CCCCCNNNNN
961 NNANNNNNNN NNN
//
Primer: pGCAP_R
Region: T7 pro, insert 3'
Sequence file: RDB18220_B0Fbb.seq check
>D07168A3_B0Fb_1_pGCAP_R_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGGNNNNN CGTAATACGA CTCNCTATAG GGCTGCAGAA
   61 TCTAGATTAC AGCGCCTTTC TCCGTTTTTC TGCCACTTCC TTCCAATACT GAGAGGATGG
  121 ATTTTCTTTA ATCATAAGAT CAAATGACTC CTGGGTGACT CCTCCAAGAT TTTTATTTTC
  181 ACTAGATTCT GGGACAATAA CCCCAGGGCT GGAAGTTGTA GATGTTAAGT GGTCATTCCG
  241 ATGTTTCCTT TTGGACAAGC CTGCGGACAG CTCATTTTCT CTTCCAACAA GAGATCCAGA
  301 TGCAGAAGGC TGAATCATCT TCAGAGTTCT TCTTGGGACA GAACTATTCT TTATATTCTC
  361 TTTGATTTCT TCTTGTTTCT GCTTCATACT GGGATTCATC TCGAGCGGCC GCCAGTGTGA
  421 TGGATATCCC TTATTCAGGT TCTTTTCAGG CAGCAGGCTG TACCTGGCCA CAGCCCCTGC
  481 GTAGAGCTTG ACAGGGCGTC CTTCGGGCTT CTCCAGGATC TCGATCCGGT GGTCCACGAA
  541 GTGGTAATCT GGCATCTTCT CCACCTTATT TTTGGGAATA TAAGAAGTGT TGAAATCGAC
  601 TCTGAGGTGA CCACCATCCT TCAACAGCAG GGCCATATTC ACGTCGCCCT TCAGCACTCC
  661 ATCGTTTTTA TCGTCCTTGT ACATCACCTC GGTGGAAGGT TCCCATTTGA CTGTTTCCTT
  721 TTGCATGACT GGGCCGTGGG GGGGGAACTT TCCGTTGAAT TGTATTTTAT AATGGAAGCA
  781 GTCCCCTTGA ACGCTGATAT CGNACTCAAC GGTTGAGACN CCGCCGTCTT CATAGGTCAT
  841 TGTTCNTTTN CATGNNNATC CCCCGCCACT GAANGNCTGT TTGAANAAGT CTGGNATGTT
  901 ACCGGGGNAC NNGGNNAACG CCNNATTGNC GTANTGAANN GNTGGGGNNA GNANNTCGAA
961 NCTGANNNNN NNNGTCTTTN CNNNCNNNNN CNNNCNNNNN NNNNN
//
Primer: Azalea5B_F
Region: insert mid
Sequence file: RDB18220_B0Fbc.seq check
>D07168A3_B0Fb_1_Azalea5B_F_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGGNCTTG AGGTGCCACC TTACCNTTTC
   61 GTGGATCATA AACTCGACCT GCTCAGTCAT AACACCGACG GAGCCACCTT TGAGGAGTTC
  121 GAACAAGGTG AAATCGCTCA CGCGCACTTG AGTAAACTGG CCGGATATCC ATCACACTGG
  181 CGGCCGCTCG AGCCCAGCCC CGCCAGGCCC GCACTCCGCG CCCCGGCCTC CGCTACCAGT
  241 GGCAGCCGCA AGCGCGCCCG CCCGCCCGCC GCCCCCGGAC GCGACCAGGC CAGGCCACCG
  301 GCCCGCAGGA GACTGCGGCT GTCGGTGGAC GAGGTTTCCA GCCCCAGTAC CCCCGAGGCC
  361 CCAGACATCC CAGCCTGCCC TTCTCCGGGC CAGAAGATAA AGAAATCCAC CCCGGCAGCA
  421 GGTCAGCCGC CCCACCTGAC ATCCGCGCAG GACCAGGACA CCATCACCGG TCGCGCCAAG
  481 CGCGGCAGCG GCGCCACCAA CTTCAGCCTG CTGAAGCAGG CCGGCGACGT GGAGGAGAAC
  541 CCCGGCCCCC GCGAATTCAT GAGTAAACTC CAGAAAGGTG TGGAGAAAGA GATGAAGATT
  601 AAGCTCACTA TGAACTGCAC CGTGAACGAA CGGAACTTTG TGATCACAGG GCAGGGGGCT
  661 GGGGAACCTT ATGATGGAAN TCAGACACTT TACCTGACAG TGGAGGGCGG AAAGACACTG
  721 AACTTCAGCT TCGATGTACT GACCCCAGCC TTTCAGTACG GCAATAGGGC GTTTACCAAG
  781 TACCCCGGTA ACATACCAGA CTTCTTCAAA CAGACCTTCA GTGGCGGGGG ANACACATGG
  841 AAAAAGANNA ATGACNTATG AAGACGGNNG GCGNNTCNNC CGTTGANTCC GATATCANCG
  901 TTNCAAGGGG ANTGCTTCCN NTNANANNTA NNATTCNNCG GAAANTNNCC NCCCCNNNGG
  961 CCCANNCNNN CAAAANGAAA NNGNCNAATG GGNAANCNTT NNCNNNNNNG NNNTGNNNNA
1021 NNNNNNNNNA NNNNNNNNNN
//
Primer: tFucci_f1
Region: insert mid
Sequence file: RDB18220_B0Fbd.seq check
>D07168A3_B0Fb_1_tFucci_f1_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNCN NNNNTTCTCC NGGCNNNNAA GNNAANNGNA ANTCCACCCC
   61 GGCAGCAGGT CAGCCGCCCC ACCTGACATC CGCGCNNGAC CAGGACACCA TCACCGGTCG
  121 CGCCAAGCGC GGCAGCGGCG CCACCAACTT CAGCCTGCTG AAGCAGGCCG GCGACGTGGA
  181 GGAGAACCCC GGCCCCCGCG AATTCATGAG TAAACTCCAG AAAGGTGTGG AGAAAGAGAT
  241 GAAGATTAAG CTCACTATGA ACTGCACCGT GAACGAACGG AACTTTGTGA TCACAGGGCA
  301 GGGGGCTGGG GAACCTTATG ATGGAACTCA GACACTTTAC CTGACAGTGG AGGGCGGAAA
  361 GACACTGAAC TTCAGCTTCG ATGTACTGAC CCCAGCCTTT CAGTACGGCA ATAGGGCGTT
  421 TACCAAGTAC CCCGGTAACA TACCAGACTT CTTCAAACAG ACCTTCAGTG GCGGGGGATA
  481 CACATGGAAA AGAACAATGA CCTATGAAGA CGGCGGCGTC TCAACCGTTG AGTCCGATAT
  541 CAGCGTTCAA GGGGACTGCT TCCATTATAA AATACAATTC AACGGAAAGT TCCCCCCCCA
  601 CGGCCCAGTC ATGCAAAAGG AAACAGTCAA ATGGGAACCT TCCACCGAGG TGATGTACAA
  661 GGACGATAAA AACGATGGAG TGCTGAAGGG CGACGTGAAT ATGGCCCTGC TGTTGAAGGA
  721 TGGTGGTCAC CTCAGAGTCG ATTTCAACAC TTCTTATATT CCNAAAAATA AGGTGGANAA
  781 GATGCCAGAT TANCACTTCN TGGANCANCG GATCGANATC CTGGANAANC CCGAAGGANG
  841 CCCNGTCNNG CTCTACGCNN GGGCTGTGGN CAGGNTACNN NCNGCNGNCT GAAAANAANN
  901 TGAATAAGGN ATNTCCNTCA CACNGGNCNG NCGCNCGAGA TGNATCCNNG TATGANNNNG
  961 AAANNAGAAN AAATCNAAGA NAANNATNAA NNANNGTTNN GNNNCCNNNN AANAANNNNN
1021 NNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ando, R., Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

2020.12.17

GNP_filter3_RDBDEP_html_201216.pl