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pRSETB-BReleaCa (#RDB15908)

Bacteria expression vector of UnaG/CaM hybrid protein, BReleaCa, capable of binding to bilirubin and calcium.

Clone info. Expression vector of UnaG/CaM hybrid protein, BReleaCa, capable of binding to bilirubin and calcium.
Comment UnaG(1-84)-ratCaM-UnaG(85-139)
Vector backbone pRSET B (Plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Growth conditions 37c, LB + Amp.
Gene/insert name Anguilla japonica (Japanese eel) UnaG cDNA Rat Calm2 cDNA
Depositor|Developer Miyawaki, Atsushi | Shitashima, Yoh |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Shitashima, Y. et al., Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018).
提供案内 (日本国内) [open/close]

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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Shitashima, Y. et al., Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15908 pRSETB-BReleaCa Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pRSETB-BReleaCa was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15908).

Reference section:

Shitashima, Y., Shimozawa, T., Asahi, T., Miyawaki, A., A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin. Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018). PMID 29395087. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Shitashima, Y., A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin. Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018). PMID 29395087. [link to RRC of NBRP]
reference Chen, T.W., Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature 499 (7458): 295-300 (2013). PMID 23868258.