RDB15908 - pRSETB-BReleaCa

Bacteria expression vector of UnaG/CaM hybrid protein, BReleaCa, capable of binding to bilirubin and calcium.

Distribution information

Catalog number	RDB15908
Resource name	pRSETB-BReleaCa
Clone info.	Expression vector of UnaG/CaM hybrid protein, BReleaCa, capable of binding to bilirubin and calcium. UnaG(1-84)-ratCaM-UnaG(85-139)
Vector backbone	pRSET B (Plasmid)
Size of vector backbone	2.9 kb
Selectable markers	Amp^r
Gene/insert name	Anguilla japonica (Japanese eel) UnaG cDNA Rat Calm2 cDNA
Depositor\|Developer	Miyawaki, Atsushi \| ShitaShima, Yoh \|

Other clones in our bank	Rat Calm2 (KEGG orthology K02183) \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Shitashima, Y. et al., A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin. Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki

Terms and conditions set forth by the DEPOSITOR

1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Shitashima, Y. et al., A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin. Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018).
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.

Ordering

Please visit Information of Request for Distribution.[link]
Order form [Credit Card Payment

] [Bank Transfer or Check Payment

]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word

]
Approval form [Word]

by Dr. Atsushi Miyawaki

Distribution information (domestic users)

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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Shitashima, Y. et al., A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin. Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。

References and tips

Sequence information

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB15908	pRSETB-BReleaCa	Under QC test. Please contact us.	DNA solution

original	Shitashima, Y., A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin. Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018). PMID 29395087.

Featured content	Sensor and Visualization (English text)

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

original	Shitashima, Y., A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin. Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018). PMID 29395087.
reference	Chen, T.W., Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature 499 (7458): 295-300 (2013). PMID 23868258.