RDB15908 - pRSETB-BReleaCa

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pRSETB-BReleaCa (#RDB15908)

Bacteria expression vector of UnaG/CaM hybrid protein, BReleaCa, capable of binding to bilirubin and calcium.

Clone info.	Expression vector of UnaG/CaM hybrid protein, BReleaCa, capable of binding to bilirubin and calcium.
Comment	UnaG(1-84)-ratCaM-UnaG(85-139)
Vector backbone	pRSET B (Plasmid)
Size of vector backbone	2.9 kb
Selectable markers	Amp^r
Gene/insert name	Anguilla japonica (Japanese eel) UnaG cDNA Rat Calm2 cDNA
Depositor\|Developer	Miyawaki, Atsushi \| Shitashima, Yoh \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Shitashima, Y. et al., A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin. Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018).
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.

Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Shitashima, Y. et al., A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin. Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Shitashima, Y. et al., A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin. Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB15908	pRSETB-BReleaCa	Under QC test. Please contact us.	DNA solution

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pRSETB-BReleaCa was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15908).

Reference section:

Shitashima, Y., Shimozawa, T., Asahi, T., Miyawaki, A., A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin. Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018). PMID 29395087. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original	Shitashima, Y., A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin. Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018). PMID 29395087. [link to RRC of NBRP]
reference	Chen, T.W., Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature 499 (7458): 295-300 (2013). PMID 23868258.

Resource search

Link

Other resources in our bank

Rat Calm2 and ortholog

External Database
Anguilla japonica (Japanese eel) UnaG

Rat Calm2

Reference sequence