Sequencing and PCR primers for primer information.
Expression vector of dimeric Keima-Red (dKeima).
| Catalog number | RDB15237 |
|---|---|
| Resource name | dKeima (h)/pCS2 |
| Clone info. | Expression vector of dimeric Keima-Red (dKeima). |
| Vector backbone | pCS2+ (Plasmid) |
| Gene/insert name | Montipora. sp RFP cDNA |
| Depositor | Miyawaki, Atsushi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. |
|---|---|
| Additional terms and conditions for distribution | The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. |
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Approval form by depositor [Word] |
| Remarks | Please obtain written approval of the depositor. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15237 | dKeima (h)/pCS2 | DNA solution |
Electronic file
Featured content
| Featured content | Dr. Miyawaki's resources (English text) |
|---|---|
| Featured content | Fluorescent protein resources (English text) |
| Featured content | Fluorescent protein resources (Japanese text) |
References
| original | Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15237_A7H9p1.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
| Primer: pAxCALN_LF1 | RDB15237_A7H9a.seq |
|---|---|
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>D04498E4-1_A7H9_pAxCALNL_F1_E03_15_ABI24.ab1 1 TTCAAGCTAT CTTATCATGT CTGGATCTAC GTAATACGAC TCACTATAGT TCTAGAGGCT 61 CGAGAGGCCT TGAATTCTTA GCCCAGCAGG GAGTGGCGGG CGATGGCGAT CTCGCACTGC 121 TCCACGGAGG TGTAGTCCCT GTTGTGGCTG GTCACGTCCA GCTTCCTGTC GATGTAGTGG 181 TAGCCGGGCA TCCTCACGGG CTTCTTGGCC TTGTAGGTGG ACTTGAACTC GCACAGGTAG 241 TGGCCGCCGC CCTCCAGCTT CAGGGCCATG TAGTCGTTGC CGATCAGCAT TCCGTCCCTG 301 GCGAACAGCC TCTCGGTGCT GGGCTCCCAG CCCTGGGTCT TCTTCTGCAT CACGGGGCCG 361 TTGGGGGGGA AGTTGGTGCC GCTGATCTTC ACGTTGTAGA TGAAGCAGTT GCCCTGGATG 421 CTGGAGTCGT TGCTCACGGT GCACACGGCG CCGTCCTCGA AGTTCATGGT CCTCTCCCAG 481 GTGTAGCCCT CGGGGAAGCT CTGCTTCACG TAGTCGGGGA TGTCCTCGGG GTACTTGGTG 541 AAGGGGATGC TGCCGTACTG GAACAGGGGG GACAGGATGT CCCAGGCGAA GGGCAGGGGG 601 CCGCCCTTGG TCACGGTCAG CTTCACGGTC TGCTCGCCCT CGTAGGGCTT GCCCTTGCCG 661 TCGCCCTCCA CCTCGAAGTA GTGGCCGTTC ACGGTGCCGG ACATGTACAC CTTGTAGGTC 721 ATCTGCTTGG CGATCACGCT CACCATGGCG GATCCTGCAA AAAGAACAAG TAGCTTGTAT 781 TCTATAGTGT CACCTAAATC AAGCTTGCTC CGACGTCCCC AGGCAGAATG GCGGTTCCCT 841 AAACGAGCAT TGCTTATATA GACCTCCCAT TAGGCACGCC TACCGCCCAT TTACGTCAAT 901 GGAACGCCCA TTTGCGTCAT TGCCCCTCCC CATTGACGTC AATGGGGGAT GTACTTGGCA 961 GCCATCGCGG GCCATTTACC GCCATTGACG TCAATGGGAG TACTGCCCAA TGTACCCTGG 1021 CGTACTTTCC AATAGTAATG TACTTTGCCA GTTACTATTT AATAGATATT GAATGTACCT 1081 GCAGTGGGGC ATTTAACCGT CATGACGTTC AATAGGGGGC CGTGAGACGG AATAATTGAA 1141 TGGGGACCAA T // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919. |
|---|
2019.02.26
GNP_filter3_RDBDEP_html_190131.pl