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dKeima (h)/pCS2

Expression vector of dimeric Keima-Red (dKeima).

Catalog number RDB15237
Resource name dKeima (h)/pCS2
Clone info. Expression vector of dimeric Keima-Red (dKeima).
Vector backbone pCS2+ (Plasmid)
Gene/insert name Montipora. sp RFP cDNA
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15237 dKeima (h)/pCS2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

References

original Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15237_A7H9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCALN_LF1 RDB15237_A7H9a.seq
>D04498E4-1_A7H9_pAxCALNL_F1_E03_15_ABI24.ab1
    1 TTCAAGCTAT CTTATCATGT CTGGATCTAC GTAATACGAC TCACTATAGT TCTAGAGGCT
   61 CGAGAGGCCT TGAATTCTTA GCCCAGCAGG GAGTGGCGGG CGATGGCGAT CTCGCACTGC
  121 TCCACGGAGG TGTAGTCCCT GTTGTGGCTG GTCACGTCCA GCTTCCTGTC GATGTAGTGG
  181 TAGCCGGGCA TCCTCACGGG CTTCTTGGCC TTGTAGGTGG ACTTGAACTC GCACAGGTAG
  241 TGGCCGCCGC CCTCCAGCTT CAGGGCCATG TAGTCGTTGC CGATCAGCAT TCCGTCCCTG
  301 GCGAACAGCC TCTCGGTGCT GGGCTCCCAG CCCTGGGTCT TCTTCTGCAT CACGGGGCCG
  361 TTGGGGGGGA AGTTGGTGCC GCTGATCTTC ACGTTGTAGA TGAAGCAGTT GCCCTGGATG
  421 CTGGAGTCGT TGCTCACGGT GCACACGGCG CCGTCCTCGA AGTTCATGGT CCTCTCCCAG
  481 GTGTAGCCCT CGGGGAAGCT CTGCTTCACG TAGTCGGGGA TGTCCTCGGG GTACTTGGTG
  541 AAGGGGATGC TGCCGTACTG GAACAGGGGG GACAGGATGT CCCAGGCGAA GGGCAGGGGG
  601 CCGCCCTTGG TCACGGTCAG CTTCACGGTC TGCTCGCCCT CGTAGGGCTT GCCCTTGCCG
  661 TCGCCCTCCA CCTCGAAGTA GTGGCCGTTC ACGGTGCCGG ACATGTACAC CTTGTAGGTC
  721 ATCTGCTTGG CGATCACGCT CACCATGGCG GATCCTGCAA AAAGAACAAG TAGCTTGTAT
  781 TCTATAGTGT CACCTAAATC AAGCTTGCTC CGACGTCCCC AGGCAGAATG GCGGTTCCCT
  841 AAACGAGCAT TGCTTATATA GACCTCCCAT TAGGCACGCC TACCGCCCAT TTACGTCAAT
  901 GGAACGCCCA TTTGCGTCAT TGCCCCTCCC CATTGACGTC AATGGGGGAT GTACTTGGCA
  961 GCCATCGCGG GCCATTTACC GCCATTGACG TCAATGGGAG TACTGCCCAA TGTACCCTGG
 1021 CGTACTTTCC AATAGTAATG TACTTTGCCA GTTACTATTT AATAGATATT GAATGTACCT
 1081 GCAGTGGGGC ATTTAACCGT CATGACGTTC AATAGGGGGC CGTGAGACGG AATAATTGAA
1141 TGGGGACCAA T
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919.

2019.02.26

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