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dKeima (h)/pCS2

Expression vector of dimeric Keima-Red (dKeima).

Catalog number RDB15237
Resource name dKeima (h)/pCS2
Clone info. Expression vector of dimeric Keima-Red (dKeima).
Vector backbone pCS2+ (Plasmid)
Gene/insert name Montipora. sp RFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Montipora. sp RFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15237 dKeima (h)/pCS2 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15237_A7H9p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxCALN_LF1
            Sequence file: RDB15237_A7H9a.seq check
            >D04498E4-1_A7H9_pAxCALNL_F1_E03_15_ABI24.ab1
                1 TTCAAGCTAT CTTATCATGT CTGGATCTAC GTAATACGAC TCACTATAGT TCTAGAGGCT
               61 CGAGAGGCCT TGAATTCTTA GCCCAGCAGG GAGTGGCGGG CGATGGCGAT CTCGCACTGC
              121 TCCACGGAGG TGTAGTCCCT GTTGTGGCTG GTCACGTCCA GCTTCCTGTC GATGTAGTGG
              181 TAGCCGGGCA TCCTCACGGG CTTCTTGGCC TTGTAGGTGG ACTTGAACTC GCACAGGTAG
              241 TGGCCGCCGC CCTCCAGCTT CAGGGCCATG TAGTCGTTGC CGATCAGCAT TCCGTCCCTG
              301 GCGAACAGCC TCTCGGTGCT GGGCTCCCAG CCCTGGGTCT TCTTCTGCAT CACGGGGCCG
              361 TTGGGGGGGA AGTTGGTGCC GCTGATCTTC ACGTTGTAGA TGAAGCAGTT GCCCTGGATG
              421 CTGGAGTCGT TGCTCACGGT GCACACGGCG CCGTCCTCGA AGTTCATGGT CCTCTCCCAG
              481 GTGTAGCCCT CGGGGAAGCT CTGCTTCACG TAGTCGGGGA TGTCCTCGGG GTACTTGGTG
              541 AAGGGGATGC TGCCGTACTG GAACAGGGGG GACAGGATGT CCCAGGCGAA GGGCAGGGGG
              601 CCGCCCTTGG TCACGGTCAG CTTCACGGTC TGCTCGCCCT CGTAGGGCTT GCCCTTGCCG
              661 TCGCCCTCCA CCTCGAAGTA GTGGCCGTTC ACGGTGCCGG ACATGTACAC CTTGTAGGTC
              721 ATCTGCTTGG CGATCACGCT CACCATGGCG GATCCTGCAA AAAGAACAAG TAGCTTGTAT
              781 TCTATAGTGT CACCTAAATC AAGCTTGCTC CGACGTCCCC AGGCAGAATG GCGGTTCCCT
              841 AAACGAGCAT TGCTTATATA GACCTCCCAT TAGGCACGCC TACCGCCCAT TTACGTCAAT
              901 GGAACGCCCA TTTGCGTCAT TGCCCCTCCC CATTGACGTC AATGGGGGAT GTACTTGGCA
              961 GCCATCGCGG GCCATTTACC GCCATTGACG TCAATGGGAG TACTGCCCAA TGTACCCTGG
             1021 CGTACTTTCC AATAGTAATG TACTTTGCCA GTTACTATTT AATAGATATT GAATGTACCT
             1081 GCAGTGGGGC ATTTAACCGT CATGACGTTC AATAGGGGGC CGTGAGACGG AATAATTGAA
            1141 TGGGGACCAA T
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl