RDB15237 - dKeima (h)/pCS2

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dKeima (h)/pCS2 (#RDB15237)

Expression vector of dimeric Keima-Red (dKeima).

Clone info.	Expression vector of dimeric Keima-Red (dKeima).
Vector backbone	pCS2+ (Plasmid)
Gene/insert name	Montipora. sp RFP cDNA
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.

Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15237	dKeima (h)/pCS2	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The dKeima (h)/pCS2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15237).

Reference section:

Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB19809_B2L5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F (pr 506) Region: CMV IE94 pro,SP6 pro,insert 5' Sequence file: RDB19809_B2L5a.seq
>D04498K5_B2L5_1_pCMV-IE94_F_E02_14_ABI24.ab1 1 NNNNNNNNNN NNNNGNNNNN ATGGGNNGNC TATATAAGCA ATGCTCGTTT AGGGAACCGC 61 CATTCTGCCT GGGGACGTCG GAGCAAGCTT GATTTAGGTG ACACTATAGA ATACAAGCTA 121 CTTGTTCTTT TTGCAGGATC CGCCATGGTG AGCGTGATCG CCAAGCAGAT GACCTACAAG 181 GTGTACATGT CCGGCACCGT GAACGGCCAC TACTTCGAGG TGGAGGGCGA CGGCAAGGGC 241 AAGCCCTACG AGGGCGAGCA GACCGTGAAG CTGACCGTGA CCAAGGGCGG CCCCCTGCCC 301 TTCGCCTGGG ACATCCTGTC CCCCCTGTTC CAGTACGGCA GCATCCCCTT CACCAAGTAC 361 CCCGAGGACA TCCCCGACTA CGTGAAGCAG AGCTTCCCCG AGGGCTACAC CTGGGAGAGG 421 ACCATGAACT TCGAGGACGG CGCCGTGTGC ACCGTGAGCA ACGACTCCAG CATCCAGGGC 481 AACTGCTTCA TCTACAACGT GAAGATCAGC GGCACCAACT TCCCCCCCAA CGGCCCCGTG 541 ATGCAGAAGA AGACCCAGGG CTGGGAGCCC AGCACCGAGA GGCTGTTCGC CAGGGACGGA 601 ATGCTGATCG GCAACGACTA CATGGCCCTG AAGCTGGAGG GCGGCGGCCA CTACCTGTGC 661 GAGTTCAAGT CCACCTACAA GGCCAAGAAG CCCGTGAGGN TGCCCGGCTA CCACTACATC 721 GACAGGAAGC TGGACGTGAC CAGCCACAAC AGGGACTACA CCTCCGTGGA GCAGTGCGAG 781 ATCGCCATCG CCCGCCACTC CCTGCTGGGC TAAGAATTCA AGGCCTCTCG AGCCTCTAGA 841 ACTATAGTGA GTCGNATTAC GTAGATCCNG ACATGATAAG ATACATTGAT GANTTNGGNN 901 AAACCNCAAC TAGAATGCAG NGAAAAAAAN TGCTTTATTN GNGAAANTNG NGNTGCNATN 961 GCTTTNNTTG NNNNNNNTNA NNNNCNNNNN NNAANNNNNT NNNNNNNNNN //
Primer: reverse-2 (pr 100) Region: SV40 pA,insert 3' Sequence file: RDB19809_B2L5b.seq
>D04498K5_B2L5_1_reverse-2_F02_17_ABI24.ab1 1 NNNNNNNNNN NNNNNGCNNN GCGCNNNNNN AACCCTCNCT AAAGGGAACA AAAGCTGGGT 61 ACCGGGCCCA ATGCATTGGC GCCGCGGCCG CGAATTAAAA AACCTCCCAC ACCTCCCCCT 121 GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA 181 TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA 241 TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGA TCTACGTAAT 301 ACGACTCACT ATAGTTCTAG AGGCTCGAGA GGCCTTGAAT TCTTAGCCCA GCAGGGAGTG 361 GCGGGCGATG GCGATCTCGC ACTGCTCCAC GGAGGTGTAG TCCCTGTTGT GGCTGGTCAC 421 GTCCAGCTTC CTGTCGATGT AGTGGTAGCC GGGCATCCTC ACGGGCTTCT TGGCCTTGTA 481 GGTGGACTTG AACTCGCACA GGTAGTGGCC GCCGCCCTCC AGCTTCAGGG CCATGTAGTC 541 GTTGCCGATC AGCATTCCGT CCCTGGCGAA CAGCCTCTCG GTGCTGGGCT CCCAGCCCTG 601 GGTCTTCTTC TGCATCACGG GGCCGTTGGG GGGGAAGTTG GTGCCGCTGA TCTTCACGTT 661 GTAGATGAAG CAGTTGCCCT GGATGCTGGA GTCGTTGCTC ACGGTGCACA CGGCGCCGTC 721 CTCGAAGTTC ATGGTCCTCT CCCAGGTGTA GCCCTCGGGG AAGCTCTGCT TCACGTAGTC 781 GGGGATGTCC TCGGGGTACT TGGTGAAGGG GATGCTGCCG TACTGGAACA NGGGGGACAG 841 GATGTCNCAG GCGAANGGCA NGGGGNCGCC CTNNGTCACN GNCAGCTTCA CGGNCTGCTC 901 GCCNNCNNAG GGCTTGNCNT TGCNNNCGCN NTNNNNNNCN AANTANTGGN NNTTCNNNGN 961 GNNNGNNNTG NNNNNNNNNN //
Primer: Amp_R (pr 8) Region: AmpR pro,f1 ori Sequence file: RDB19809_B2L5c.seq
>D04498K5_B2L5_1_Amp_R_G02_20_ABI24.ab1 1 NNNNNNNNNN NNNTNNTTGN NGCATTTATN NGGGTTATTG TCTCATGAGC GGATACATAT 61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 121 CACCTAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG 181 CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC 241 CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA 301 CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC 361 ACCCTAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG 421 GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA 481 GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC 541 CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCCCATTCGC CATTCANGCT 601 GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC NGTCNANCGC 661 CNATTCNATA TGGCGTATAT GGACTCNTGC CNATNCAATA TGGTGGNTCT GGANCTGTGN 721 CNATTCNNTA TGNNGTATAT GGNCTCNTGN NNNNTCNNTA TNNNGNNNNT NNACCNNNNC 781 NNNNTNNNNN TNNNNNANNN NNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919. [link to RRC of NBRP]

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External Database
Montipora. sp RFP

Reference sequence