RDB15442 - mCherry-hCdt1(1/100)/pCSII-EF

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mCherry-hCdt1(1/100)/pCSII-EF (#RDB15442)

Lentivirus vector plasmid of mCherry-hCdt1(1/100). EF-1 alpha promoter.

Clone info.	Lentivirus vector plasmid of mCherry-hCdt1(1/100). EF-1 alpha promoter.
Vector backbone	CSII-EF-MCS (RDB04378) (plasmid)
Size of vector backbone	9.1 kb
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name	Human CDT1 cDNA Discosoma sp. RFP cDNA
Reference of insert sequence	LC192885 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose)[Word] [Example of MTA ]
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Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
Additional terms and conditions:
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.

Remarks Remember that you will be working with samples containing infectious virus.
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose)[Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks	Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] [MTAの記入例 ] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. MTAに書く付加的使用条件：レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。提供条件は英文のまま記載してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15442	mCherry-hCdt1(1/100)/pCSII-EF	DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The mCherry-hCdt1(1/100)/pCSII-EF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15442).

Reference section:

Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15442_A7Jvp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,mCherry-hCdt1(1/100) 5' Sequence file: RDB15442_A7Jva.seq
>D05018A2_A7Jv_2_EF1a-C_F_A01_01_ABI24.ab1 1 CCTTTGGGAA TTTGGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG 61 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAAC ACGCTACCGG TCTCGAGAAT 121 TCGCCACCAT GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC 181 GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG 241 GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC 301 CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG 361 TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT 421 GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC 481 TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG 541 GCCCCGTAAT GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG 601 AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT 661 ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT 721 ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC 781 AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGGGAT 841 ATCCATCACA CTGGCGGCCG CTCGAGATGG AGCAGCGCCG CGTCACCGAC TTCTTCGCGC 901 GCCGCCGCCC CGGGCCCCCC CCGCATCGCG CCGCCCAAGC TGGCCTGCCG CACCCCCAGC 961 CCCGCCAGGC CCGCACTCCG CGCCCCGGCC TCCGCTACCA GTGGCAGCCG CAAGCGCGCC 1021 CGCCCGGCCG CCGCCCCCGG ACGCGAACCA GGCCAGGGCC ACCGGGCCCG CAGAGACTGC 1081 GGCTGGTCGG TGGAACGAGT TTCAGCCCCA AGTTACCCCC GAGGCCTCAG AAACATTCCC 1141 AGGCCTGCCC GTTC //
Primer: PRE_rev (Pr0250) Region: PRE,mCherry-hCdt1(1/100) 3' Sequence file: RDB15442_A7Jvb.seq
>D05018A2_A7Jv_2_PRE_rev_B01_04_ABI24.ab1 1 AGGAATCACA TAGCGTAAAG GAGCAACATA GTTAAGAATA CCAGTCAATC TTTCACAAAT 61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGATTAT 121 TTCTTTATCT TCTGGCCCGG AGAAGGGCAG GCTGGGATGT CTGGGGCCTC GGGGGTACTG 181 GGGCTGGAAA CCTCGTCCAC CGACAGCCGC AGTCTCCTGC GGGCCGGTGG CCTGGCCTGG 241 TCGCGTCCGG GGGCGGCGGG CGGGCGGGCG CGCTTGCGGC TGCCACTGGT AGCGGAGGCC 301 GGGGCGCGGA GTGCGGGCCT GGCGGGGCTG GGGGTGCGGC AGGCCAGCTT GGGCGGCGCG 361 ATGCGGGGGG GCCCGGGGCG GCGGCGCGCG AAGAAGTCGG TGACGCGGCG CTGCTCCATC 421 TCGAGCGGCC GCCAGTGTGA TGGATATCCC TTGTACAGCT CGTCCATGCC GCCGGTGGAG 481 TGGCGGCCCT CGGCGCGTTC GTACTGTTCC ACGATGGTGT AGTCCTCGTT GTGGGAGGTG 541 ATGTCCAACT TGATGTTGAC GTTGTAGGCG CCGGGCAGCT GCACGGGCTT CTTGGCCTTG 601 TAGGTGGTCT TGACCTCAGC GTCGTAGTGG CCGCCGTCCT TCAGCTTCAG CCTCTGCTTG 661 ATCTCGCCCT TCAGGGCGCC GTCCTCGGGG TACATCCGCT CGGAGGAGGC CTCCCAGCCC 721 ATGGTCTTCT TCTGCATTAC GGGGCCGTCG GAGGGGAAGT TGGTGCCGCG CAGCTTCACC 781 TTGTAGATGA ACTCGCCGTC CTGCAGGGAG GAGTCCTGGG TCACGGTCAC CACGCCGCCG 841 TCCTCGAAGT TCATCACGCG CTCCCACTTG AAGCCCTCGG GGAAGGACAG CTTCAAGTAG 901 TCGGGGATGT CGGCGGGGTG CTTCACGTAG GCCCTTGGAG CCGTACATGA ACTGAGGGGA 961 CAGGGATGTC CCAGGCGAAG GCAGGGGCCA CCCTTTGGTC ATCATCAGCT GCGGTCTGGT 1021 GCCTTCGGTA GGGCGTCTCG TCTCGCCATC GATCTCGACT CGTGTCGGTC ACGAGTCATC 1081 ATGTGCACAT GGAGCGCAAT GGACCTTCCA TTGAG //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5'LTR,HIV-1 psi Sequence file: RDB15442_A7Jvc.seq
>D05018A2_A7Jv_2_CMV-Forward_C01_07_ABI24.ab1 1 CGGTTCCACC TCCGGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT 61 ATATAAGCAG CGCGTTTTGC CTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG 121 GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG 181 CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC 241 TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT GGCGCCCGAA CAGGGACTTG AAAGCGAAAG 301 GGAAACCAGA GGAGCTCTCT CGACGCAGGA CTCGGCTTGC TGAAGCGCGC ACGGCAAGAG 361 GCGAGGGGCG GCGACTGGTG AGTACGCCAA AAATTTTGAC TAGCGGAGGC TAGAAGGAGA 421 GAGATGGGTG CGAGAGCGTC AGTATTAAGC GGGGGAGAAT TAGATCGCGA TGGGAAAAAA 481 TTCGGTTAAG GCCAGGGGGA AAGAAAAAAT ATAAATTAAA ACATATAGTA TGGGCAAGCA 541 GGGAGCTAGA ACGATTCGCA GTTAATCCTG GCCTGTTAGA AACATCAGAA GGCTGTAGAC 601 AAATACTGGG ACAGCTACAA CCATCCCTTC AGACAGGATC AGAAGAACTT AGATCATTAT 661 ATAATACAGT AGCAACCCTC TATTGTGTGC ATCAAAGGAT AGAGATAAAA GACACCAAGG 721 AAGCTTTAGA CAAGATAGAG GAAGAGCAAA ACAAAAGTAA GACCACCGCA CAGCAAGGGC 781 CGCTGATCTT CAGACCTGGA GGAGGAGATA TGAGGGACAA TTGGAGAAGT GAATTATATA 841 AATATAAAGT AGTAAAAATT GAACCATTAG GAGTAGCACC CACCAAGGCA AAGAGAAGAG 901 TGGTGCAGAG AGAAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA 961 GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CCAGACAATT 1021 ATTGTCTGGG TATAGTGCAG CAGCCAGACA ATTTGCTGGA GGCCTATTGA GCGCAACCAG 1081 CATCTGTGCA CTCACAGTCC TGGGGGCATC AAGTAAGCCT CCCAAG //
Primer: EF1a-N_R (Pr0024) Region: EF-1 alpha pro,cPPT_CTS,RRE Sequence file: RDB15442_A7Jvf.seq
>15442_15442_A9Da_EF1a-N_R_C01_03_ABI08.ab1 1 TCCTTTTCCC GGGGGATTGT GGGCGATGTG CGCTCTGCCC ACTGACGGGC ACCGGAGCCT 61 CACGAAGCTT GAATTAATTC AAAGTGAATT TCTGCTGTCC CTGTAATAAA CCCGAAAATT 121 TTGAATTTTT GTAATTTGTT TTTGTAATTC TTTAGTTTGT ATGTCTGTTG CTATTATGTC 181 TACTATTCTT TCCCCTGCAC TGTACCCCCC AATCCCCCCT TTTCTTTTAA AATTGTGGAT 241 GAATACTGCC ATTTGTAGAT CCGTTCACTA ATCGAATGGA TCTGTCTCTG TCTCTCTCTC 301 CACCTTCTTC TTCTATTCCT TCGGGCCTGT CGGGTCCCCT CGGGGTTGGG AGGTGGGTCT 361 GAAACGATAA TGGTGAATAT CCCTGCCTAA CTCTATTCAC TATAGAAAGT ACAGCAAAAA 421 CTATTCTTAA ACCTACCAAG CCTCCTACTA TCATTATGAA TAATTTTATA TACCACAGCC 481 AATTTGTTAT GTTAAACCAA TTCCACAAAC TTGCCCATTT ATCTAATTCC AATAATTCTT 541 GTTCATTCTT TTCTTGCTGG TTTTGCGATT CTTCAATTAA GGAGTGTATT AAGCTTGTGT 601 AATTGTTAAT TTCTCTGTCC CACTCCATCC AGGTCGTGTG ATTCCAAATC TGTTCCAGAG 661 ATTTATTACT CCAACTAGCA TTCCAAGGCA CAGCAGTGGT GCAAATGAGT TTTCCAGAGC 721 AACCCCAAAT CCCCAGGGAG CTGTTGATCC TTTAGGTATC TTTCCACAGC CAGGATTCTT 781 GCCTGGAGCT GCTTGATGCC CCAGACTGTG AGTTGCAACA GATGCTGTTG CGCCTCAATA 841 GCCCTCAGCA AATTGTTCTG CTGCTGCACT ATACCAGACA ATAAATTGTC TGGCCTGTAC 901 CGTCAGCGTC ATTGACGCTG CGCCCATAGT GCTTCCTGCT GCTCCCAAGA ACCCAAAGGA 961 AACAAAGCTC CTATTCCCAC TGCTCCTTTT TTTCTCTCTG CACCACTCTT TCTCTTTGCT 1021 TTGGGTGGGT GCTACTTCCT AAATGGTTCG TTTTTACTAC TTTATATTTA TATAAATCAA 1081 CCTTCTTCCA AATGGTCCGC TCAAATCTAC TCTCAAGGTT CCTGGAAGAA ATTCCAAGAC 1141 CGGCG //
Primer: BGH_rev2 (Pr0606) Region: bGHpA,3' LTR,PRE Sequence file: RDB15442_A7Jvd.seq
>D05018A2_A7Jv_2_BGH_rev2_D01_10_ABI24.ab1 1 CATTCAGGTT AGATGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC 61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA 121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA 181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT 241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG 301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT 361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT 421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT 481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT 541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC 601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG 661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC 721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC 781 GACGGTATCG ATGCGGGGAG GCGGCCCAAA GGGAGATCCG ACTCGTCTGA GGGCGAAGGC 841 GAAGACGCGG AAGAGGCCGC AGAGCCGGCA GCAGGCCGCG GGAAGGAAAG GTCCGCTGGA 901 TTGAGGGCCG AAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGAAATCCAG GTGGCAACAC 961 AGGCGAGCAG CCCATGGAAA AGGACGTCAG CTTCCCCGAC ACACCACGGG AAATTGTCAG 1021 TGCCCACAGC CGAGCTCCTG TCCAGCAGCG GGCAGCAGCG GCGGATGAGG TCGTCGTGCC 1081 ATAGGGAGGG GGAAGCGAGT CTGGCGGAGC TGACAGTGTG GCCAATGCCT CAACCAAGTG 1141 GGGGGAATC //
Primer: SV40pro_F (Pr0110) Region: SV40 pro_ori,Zeo,SV40pA Sequence file: RDB15442_A7Jve.seq
>D05018A2_A7Jv_2_SV40pro_F_E01_13_ABI24.ab1 1 CCCTGGGCCC TTGACATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA 61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA 121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC 181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT 241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT 301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC 361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT 421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG 481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA 541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG 601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT 661 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA 721 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC 781 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC 841 GTAAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCAAC AATTCCACAC 901 AACATACGAG CCGGGAAGCA TAAAGTGTAA AGCCCTGGGG TGCCTAATGA GTGAGCTAAC 961 TCACATTAAT TGCGGTTGCG CTCACTGCCC GGCTTTCCAG TCGGGGAACC CTGTCGTGCC 1021 AGCTGCATTA ATGGATCGGG CCAACGCGCG GGGGAGAGGC GGTTTTGCGT ATTTGGGCGC 1081 TCTTCCGACT TCCTTCGCTC AACTTGAACT CGGCTGGCGC CTCGGATCGA TCAGGCTGGC 1141 GGCGGGCAGG ATAATCAAGA CGTTCACAAT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535. [link to RRC of NBRP]

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human CDT1

External Database
Human CDT1

Discosoma sp. RFP

Reference sequence