Resource data sheet
Prev. |  Lentiviral vector plasmid > 

mCherry-hCdt1(1/100)/pCSII-EF

Lentivirus expression vector of mCherry-hCdt1(1/100). EF-1 alpha promoter.

Catalog number RDB15442
Resource name mCherry-hCdt1(1/100)/pCSII-EF
Clone info. Lentivirus expression vector of mCherry-hCdt1(1/100). EF-1 alpha promoter.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Amp^r (E. coli), Zeo^r (mammalian cells).
Gene/insert name Human CDT1 cDNA Discosoma sp. RFP cDNA
Reference of nucleotide sequence LC192885 (DDBJ accession number)
Originator Miyawaki, Atsushi |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
6. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
(Japanese text [open/close])
Remarks ((Additional form)) Dr. Miyawaki's Approval
((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.
(Japanese text [open/close])

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15442_A7Jvp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB15442_A7Jva.seq
>D05018A2_A7Jv_2_EF1a-C_F_A01_01_ABI24.ab1
    1 CCTTTGGGAA TTTGGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG
   61 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAAC ACGCTACCGG TCTCGAGAAT
  121 TCGCCACCAT GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC
  181 GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG
  241 GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC
  301 CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG
  361 TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT
  421 GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC
  481 TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG
  541 GCCCCGTAAT GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG
  601 AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT
  661 ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT
  721 ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC
  781 AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGGGAT
  841 ATCCATCACA CTGGCGGCCG CTCGAGATGG AGCAGCGCCG CGTCACCGAC TTCTTCGCGC
  901 GCCGCCGCCC CGGGCCCCCC CCGCATCGCG CCGCCCAAGC TGGCCTGCCG CACCCCCAGC
  961 CCCGCCAGGC CCGCACTCCG CGCCCCGGCC TCCGCTACCA GTGGCAGCCG CAAGCGCGCC
 1021 CGCCCGGCCG CCGCCCCCGG ACGCGAACCA GGCCAGGGCC ACCGGGCCCG CAGAGACTGC
 1081 GGCTGGTCGG TGGAACGAGT TTCAGCCCCA AGTTACCCCC GAGGCCTCAG AAACATTCCC
1141 AGGCCTGCCC GTTC
//
Primer: PRE_rev RDB15442_A7Jvb.seq
>D05018A2_A7Jv_2_PRE_rev_B01_04_ABI24.ab1
    1 AGGAATCACA TAGCGTAAAG GAGCAACATA GTTAAGAATA CCAGTCAATC TTTCACAAAT
   61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGATTAT
  121 TTCTTTATCT TCTGGCCCGG AGAAGGGCAG GCTGGGATGT CTGGGGCCTC GGGGGTACTG
  181 GGGCTGGAAA CCTCGTCCAC CGACAGCCGC AGTCTCCTGC GGGCCGGTGG CCTGGCCTGG
  241 TCGCGTCCGG GGGCGGCGGG CGGGCGGGCG CGCTTGCGGC TGCCACTGGT AGCGGAGGCC
  301 GGGGCGCGGA GTGCGGGCCT GGCGGGGCTG GGGGTGCGGC AGGCCAGCTT GGGCGGCGCG
  361 ATGCGGGGGG GCCCGGGGCG GCGGCGCGCG AAGAAGTCGG TGACGCGGCG CTGCTCCATC
  421 TCGAGCGGCC GCCAGTGTGA TGGATATCCC TTGTACAGCT CGTCCATGCC GCCGGTGGAG
  481 TGGCGGCCCT CGGCGCGTTC GTACTGTTCC ACGATGGTGT AGTCCTCGTT GTGGGAGGTG
  541 ATGTCCAACT TGATGTTGAC GTTGTAGGCG CCGGGCAGCT GCACGGGCTT CTTGGCCTTG
  601 TAGGTGGTCT TGACCTCAGC GTCGTAGTGG CCGCCGTCCT TCAGCTTCAG CCTCTGCTTG
  661 ATCTCGCCCT TCAGGGCGCC GTCCTCGGGG TACATCCGCT CGGAGGAGGC CTCCCAGCCC
  721 ATGGTCTTCT TCTGCATTAC GGGGCCGTCG GAGGGGAAGT TGGTGCCGCG CAGCTTCACC
  781 TTGTAGATGA ACTCGCCGTC CTGCAGGGAG GAGTCCTGGG TCACGGTCAC CACGCCGCCG
  841 TCCTCGAAGT TCATCACGCG CTCCCACTTG AAGCCCTCGG GGAAGGACAG CTTCAAGTAG
  901 TCGGGGATGT CGGCGGGGTG CTTCACGTAG GCCCTTGGAG CCGTACATGA ACTGAGGGGA
  961 CAGGGATGTC CCAGGCGAAG GCAGGGGCCA CCCTTTGGTC ATCATCAGCT GCGGTCTGGT
 1021 GCCTTCGGTA GGGCGTCTCG TCTCGCCATC GATCTCGACT CGTGTCGGTC ACGAGTCATC
1081 ATGTGCACAT GGAGCGCAAT GGACCTTCCA TTGAG
//
Primer: CMV-Forward RDB15442_A7Jvc.seq
>D05018A2_A7Jv_2_CMV-Forward_C01_07_ABI24.ab1
    1 CGGTTCCACC TCCGGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG CGCGTTTTGC CTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG
  121 GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG
  181 CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC
  241 TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT GGCGCCCGAA CAGGGACTTG AAAGCGAAAG
  301 GGAAACCAGA GGAGCTCTCT CGACGCAGGA CTCGGCTTGC TGAAGCGCGC ACGGCAAGAG
  361 GCGAGGGGCG GCGACTGGTG AGTACGCCAA AAATTTTGAC TAGCGGAGGC TAGAAGGAGA
  421 GAGATGGGTG CGAGAGCGTC AGTATTAAGC GGGGGAGAAT TAGATCGCGA TGGGAAAAAA
  481 TTCGGTTAAG GCCAGGGGGA AAGAAAAAAT ATAAATTAAA ACATATAGTA TGGGCAAGCA
  541 GGGAGCTAGA ACGATTCGCA GTTAATCCTG GCCTGTTAGA AACATCAGAA GGCTGTAGAC
  601 AAATACTGGG ACAGCTACAA CCATCCCTTC AGACAGGATC AGAAGAACTT AGATCATTAT
  661 ATAATACAGT AGCAACCCTC TATTGTGTGC ATCAAAGGAT AGAGATAAAA GACACCAAGG
  721 AAGCTTTAGA CAAGATAGAG GAAGAGCAAA ACAAAAGTAA GACCACCGCA CAGCAAGGGC
  781 CGCTGATCTT CAGACCTGGA GGAGGAGATA TGAGGGACAA TTGGAGAAGT GAATTATATA
  841 AATATAAAGT AGTAAAAATT GAACCATTAG GAGTAGCACC CACCAAGGCA AAGAGAAGAG
  901 TGGTGCAGAG AGAAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA
  961 GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CCAGACAATT
 1021 ATTGTCTGGG TATAGTGCAG CAGCCAGACA ATTTGCTGGA GGCCTATTGA GCGCAACCAG
1081 CATCTGTGCA CTCACAGTCC TGGGGGCATC AAGTAAGCCT CCCAAG
//
Primer: BGH_rev2 RDB15442_A7Jvd.seq
>D05018A2_A7Jv_2_BGH_rev2_D01_10_ABI24.ab1
    1 CATTCAGGTT AGATGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC
   61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA
  121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA
  181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT
  241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG
  301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT
  361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT
  421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT
  481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT
  541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC
  601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG
  661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC
  721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC
  781 GACGGTATCG ATGCGGGGAG GCGGCCCAAA GGGAGATCCG ACTCGTCTGA GGGCGAAGGC
  841 GAAGACGCGG AAGAGGCCGC AGAGCCGGCA GCAGGCCGCG GGAAGGAAAG GTCCGCTGGA
  901 TTGAGGGCCG AAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGAAATCCAG GTGGCAACAC
  961 AGGCGAGCAG CCCATGGAAA AGGACGTCAG CTTCCCCGAC ACACCACGGG AAATTGTCAG
 1021 TGCCCACAGC CGAGCTCCTG TCCAGCAGCG GGCAGCAGCG GCGGATGAGG TCGTCGTGCC
 1081 ATAGGGAGGG GGAAGCGAGT CTGGCGGAGC TGACAGTGTG GCCAATGCCT CAACCAAGTG
1141 GGGGGAATC
//
Primer: SV40pro_F RDB15442_A7Jve.seq
>D05018A2_A7Jv_2_SV40pro_F_E01_13_ABI24.ab1
    1 CCCTGGGCCC TTGACATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA
  121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC
  181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT
  241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT
  301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC
  361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT
  421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG
  481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA
  541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
  601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT
  661 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
  721 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC
  781 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC
  841 GTAAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCAAC AATTCCACAC
  901 AACATACGAG CCGGGAAGCA TAAAGTGTAA AGCCCTGGGG TGCCTAATGA GTGAGCTAAC
  961 TCACATTAAT TGCGGTTGCG CTCACTGCCC GGCTTTCCAG TCGGGGAACC CTGTCGTGCC
 1021 AGCTGCATTA ATGGATCGGG CCAACGCGCG GGGGAGAGGC GGTTTTGCGT ATTTGGGCGC
 1081 TCTTCCGACT TCCTTCGCTC AACTTGAACT CGGCTGGCGC CTCGGATCGA TCAGGCTGGC
1141 GGCGGGCAGG ATAATCAAGA CGTTCACAAT
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References

original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

2017.12.11

GNP_filter3_RDBDEP_html_171106.pl