Please review the QC test results indicated by icon below as well as clone information before placing your order.

hmKO1/pRSETB

Plasmid clone of fluorescent protein monomeric Kusabira-Orange (mKO1).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381: 307-312. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381: 307-312. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

Tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15312_A7Htp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp () Region: insert Sequence file: RDB15312_A7Hta.seq

>D04498J3_A7H9_2_T7_20bp_C08_08_ABI24.ab1     1 GAAATCAGTA GAATATTTTG TTTACTTTAA GAAGGAGATA TACATATGCG GGGTTCTCAT    61 CATCATCATC ATCATGGTAT GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGGGATCTG   121 TACGACGATG ACGATAAGGA TCCGACCATG GTGAGCGTGA TCAAGCCCGA GATGAAGATG   181 AGGTACTACA TGGACGGCTC CGTCAATGGG CATGAGTTCA CAATCGAGGG TGAGGGCACA   241 GGCAGACCTT ACGAGGGACA TCAGGAGATG ACACTGCGCG TCACAATGGC CAAGGGCGGG   301 CCAATGCCTT TCGCCTTCGA CCTGGTGTCC CACGTGTTCT GTTACGGCCA CAGACCTTTT   361 ACTAAATATC CAGAAGAGAT CCCAGACTAT TTCAAGCAGG CCTTTCCTGA GGGCCTGTCC   421 TGGGAGAGGT CCCTGGAGTT CGAGGACGGC GGCTCCGCCT CCGTGAGCGC CCACATCAGC   481 CTGAGGGGCA ACACCTTCTA CCACAAGTCC AAGTTCACCG GCGTGAACTT CCCCGCCGAC   541 GGCCCCATCA TGCAGAACCA GAGCGTGGAC TGGGAGCCCT CCACCGAGAA GATCACCGCC   601 AGCGACGGCG TGCTGAAGGG CGACGTGACC ATGTACCTGA AGCTGGAGGG CGGCGGCAAC   661 CACAAGTGCC AGTTCAAGAC CACCTACAAG GCCGCCAAGA AGATCCTGAA GATGCCCGGC   721 AGCCACTACA TCAGCCACAG GCTGGTGAGG AAGACCGAGG GCAACATCAC CGAGCTGGTG   781 GAGGACGCCG TGGCCCACTC CTAAGAATTC GAAGCTTGAT CCGGCTGCTA ACAAAGCCCG   841 AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCCTTGGGG   901 CCTCTAAACG GGTCTTGAGG GGTTTTTTTG CTGAAAGGAG GAACTATATC CGGGATCTGG   961 CGTAATAGCG AAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTTGCGC AGCCCTGAAT  1021 GGGCGAAATG GGACGCGCCC TGTAGCGGGC GCATTTAAGC CGGCGCGGGT TGTGATGGGT  1081 TTACGCGCAG CCGTGAACCG GCTACACTTT GCCAGCCGAC CCCTAGGCGG GCCCGACATC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.17

GNP_filter3_RDBDEP_html_230702.pl