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hmKO1/pRSETB

Plasmid clone of fluorescent protein monomeric Kusabira-Orange (mKO1).

Catalog number RDB15312
Resource name hmKO1/pRSETB
Clone info. Plasmid clone of fluorescent protein monomeric Kusabira-Orange (mKO1).
Vector backbone Plasmid
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381: 307-312.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Approval form by depositor [Word]
Remarks
Please obtain written approval of the depositor.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15312 hmKO1/pRSETB DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15312_A7Htp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB15312_A7Hta.seq
>D04498J3_A7H9_2_T7_20bp_C08_08_ABI24.ab1
    1 GAAATCAGTA GAATATTTTG TTTACTTTAA GAAGGAGATA TACATATGCG GGGTTCTCAT
   61 CATCATCATC ATCATGGTAT GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGGGATCTG
  121 TACGACGATG ACGATAAGGA TCCGACCATG GTGAGCGTGA TCAAGCCCGA GATGAAGATG
  181 AGGTACTACA TGGACGGCTC CGTCAATGGG CATGAGTTCA CAATCGAGGG TGAGGGCACA
  241 GGCAGACCTT ACGAGGGACA TCAGGAGATG ACACTGCGCG TCACAATGGC CAAGGGCGGG
  301 CCAATGCCTT TCGCCTTCGA CCTGGTGTCC CACGTGTTCT GTTACGGCCA CAGACCTTTT
  361 ACTAAATATC CAGAAGAGAT CCCAGACTAT TTCAAGCAGG CCTTTCCTGA GGGCCTGTCC
  421 TGGGAGAGGT CCCTGGAGTT CGAGGACGGC GGCTCCGCCT CCGTGAGCGC CCACATCAGC
  481 CTGAGGGGCA ACACCTTCTA CCACAAGTCC AAGTTCACCG GCGTGAACTT CCCCGCCGAC
  541 GGCCCCATCA TGCAGAACCA GAGCGTGGAC TGGGAGCCCT CCACCGAGAA GATCACCGCC
  601 AGCGACGGCG TGCTGAAGGG CGACGTGACC ATGTACCTGA AGCTGGAGGG CGGCGGCAAC
  661 CACAAGTGCC AGTTCAAGAC CACCTACAAG GCCGCCAAGA AGATCCTGAA GATGCCCGGC
  721 AGCCACTACA TCAGCCACAG GCTGGTGAGG AAGACCGAGG GCAACATCAC CGAGCTGGTG
  781 GAGGACGCCG TGGCCCACTC CTAAGAATTC GAAGCTTGAT CCGGCTGCTA ACAAAGCCCG
  841 AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCCTTGGGG
  901 CCTCTAAACG GGTCTTGAGG GGTTTTTTTG CTGAAAGGAG GAACTATATC CGGGATCTGG
  961 CGTAATAGCG AAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTTGCGC AGCCCTGAAT
 1021 GGGCGAAATG GGACGCGCCC TGTAGCGGGC GCATTTAAGC CGGCGCGGGT TGTGATGGGT
 1081 TTACGCGCAG CCGTGAACCG GCTACACTTT GCCAGCCGAC CCCTAGGCGG GCCCGACATC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984.

2019.08.08

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