RDB15312 - hmKO1/pRSETB

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Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381: 307-312. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381: 307-312. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15312	hmKO1/pRSETB	DNA solution

Featured content	Fluorescent protein resources (English text)
Featured content	Fluorescent protein resources (Japanese text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

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hmKO1/pRSETB

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QC test results

References

Primer: T7_20bp (Pr0261) Region: 6xHis,T7 tag,Xpress™ tag,insert 5' Sequence file: RDB15312_A7H9b.seq
>D04498J3_A7H9_2_T7_20bp_C08_08_ABI24.ab1 1 NNNNNNNNNN NNNNATTTTG TNNNNNTTAA GAAGGAGNNA TACATATGCG GGGTTCTCAT 61 CATCATCATC ATCATGGTAT GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGGGATCTG 121 TACGACGATG ACGATAAGGA TCCGACCATG GTGAGCGTGA TCAAGCCCGA GATGAAGATG 181 AGGTACTACA TGGACGGCTC CGTCAATGGG CATGAGTTCA CAATCGAGGG TGAGGGCACA 241 GGCAGACCTT ACGAGGGACA TCAGGAGATG ACACTGCGCG TCACAATGGC CAAGGGCGGG 301 CCAATGCCTT TCGCCTTCGA CCTGGTGTCC CACGTGTTCT GTTACGGCCA CAGACCTTTT 361 ACTAAATATC CAGAAGAGAT CCCAGACTAT TTCAAGCAGG CCTTTCCTGA GGGCCTGTCC 421 TGGGAGAGGT CCCTGGAGTT CGAGGACGGC GGCTCCGCCT CCGTGAGCGC CCACATCAGC 481 CTGAGGGGCA ACACCTTCTA CCACAAGTCC AAGTTCACCG GCGTGAACTT CCCCGCCGAC 541 GGCCCCATCA TGCAGAACCA GAGCGTGGAC TGGGAGCCCT CCACCGAGAA GATCACCGCC 601 AGCGACGGCG TGCTGAAGGG CGACGTGACC ATGTACCTGA AGCTGGAGGG CGGCGGCAAC 661 CACAAGTGCC AGTTCAAGAC CACCTACAAG GCCGCCAAGA AGATCCTGAA GATGCCCGGC 721 AGCCACTACA TCAGCCACAG GCTGGTGANG AAGANCGANG GCAACATCAC CGANCTGGTG 781 GAGGANGCCG TGGCCCACTC CTAAGAATTC GAAGCTTGAT CCGGCTGCTA ACAAAGCCCG 841 AAAGGAAGCT GANTTGGCTG CTGCCACCNC NNANCAATAA CNANCATAAC CCCCTTNGGG 901 CCNNNNAACG GGNCTTGANG GGTTTTTTTG CTGAAAGGAN GAACTATNTN NNGNNTNNNG 961 NNNNNNNNCG NANNANNNCN GCNNNGNNNG CCNNNNNNNN NN //
Primer: f1ori_R3 (Pr0142) Region: T7 ter,insert 3' Sequence file: RDB15312_A7H9c.seq
>D04498J3_A7H9_2_f1ori_R3_D08_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNNANN GTTGGGANGG CGNNCGGTGC GGGCCTCTTC GCTATTACGC 61 CNGATCCGGA TATAGTTCCT CCTTTCAGCA AAAAACCCCT CAAGACCCGT TTAGAGGCCC 121 CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC CAACTCAGCT TCCTTTCGGG 181 CTTTGTTAGC AGCCGGATCA AGCTTCGAAT TCTTAGGAGT GGGCCACGGC GTCCTCCACC 241 AGCTCGGTGA TGTTGCCCTC GGTCTTCCTC ACCAGCCTGT GGCTGATGTA GTGGCTGCCG 301 GGCATCTTCA GGATCTTCTT GGCGGCCTTG TAGGTGGTCT TGAACTGGCA CTTGTGGTTG 361 CCGCCGCCCT CCAGCTTCAG GTACATGGTC ACGTCGCCCT TCAGCACGCC GTCGCTGGCG 421 GTGATCTTCT CGGTGGAGGG CTCCCAGTCC ACGCTCTGGT TCTGCATGAT GGGGCCGTCG 481 GCGGGGAAGT TCACGCCGGT GAACTTGGAC TTGTGGTAGA AGGTGTTGCC CCTCAGGCTG 541 ATGTGGGCGC TCACGGAGGC GGAGCCGCCG TCCTCGAACT CCAGGGACCT CTCCCAGGAC 601 AGGCCCTCAG GAAAGGCCTG CTTGAAATAG TCTGGGATCT CTTCTGGATA TTTAGTAAAA 661 GGTCTGTGGC CGTAACAGAA CACGTGGGAC ACCAGGTCGA AGGCGAAAGG CATTGGCCCG 721 CCCTTGGCCA TTGTGACGCG CAGTGTCATC TCCTGATGTC CCTCGTAAGG TCTGCCTGTG 781 CCCTCACCCT CGATTGTGAA CTCATGCCCA TTGACGGANC CGTCCATGTA GTACNNCATC 841 TTCATCTCGG GCTTGATCAC GCTCACCNNG GNCGGNNCCT TATCGTCATC GNCGTACAGA 901 TCCCGANCNN TTTGCTGNNC NCCANNCANG NNNGCCNTAC NNNGATGANG ANGATGATGA 961 GAACCNNNNN TATGNNNATN NNNNTNNNNN AANNNNANNN AANNTNNNNN NNNNN //