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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

hmKO1/pRSETB

Plasmid clone of fluorescent protein monomeric Kusabira-Orange (mKO1).

Catalog number RDB15312
Resource name hmKO1/pRSETB
Clone info. Plasmid clone of fluorescent protein monomeric Kusabira-Orange (mKO1).
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381: 307-312.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering
            Order form [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Please visit Information of Request for Distribution.[link] 
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381: 307-312.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15312 hmKO1/pRSETB DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The hmKO1/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15312).

            Reference section:

            Karasawa, S., Araki, T., Nagai, T., Mizuno, H., Miyawaki, A., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15312_A7Htp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: T7_20bp ()
            Region: insert
            Sequence file: RDB15312_A7Hta.seq check
            >D04498J3_A7H9_2_T7_20bp_C08_08_ABI24.ab1
                1 GAAATCAGTA GAATATTTTG TTTACTTTAA GAAGGAGATA TACATATGCG GGGTTCTCAT
               61 CATCATCATC ATCATGGTAT GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGGGATCTG
              121 TACGACGATG ACGATAAGGA TCCGACCATG GTGAGCGTGA TCAAGCCCGA GATGAAGATG
              181 AGGTACTACA TGGACGGCTC CGTCAATGGG CATGAGTTCA CAATCGAGGG TGAGGGCACA
              241 GGCAGACCTT ACGAGGGACA TCAGGAGATG ACACTGCGCG TCACAATGGC CAAGGGCGGG
              301 CCAATGCCTT TCGCCTTCGA CCTGGTGTCC CACGTGTTCT GTTACGGCCA CAGACCTTTT
              361 ACTAAATATC CAGAAGAGAT CCCAGACTAT TTCAAGCAGG CCTTTCCTGA GGGCCTGTCC
              421 TGGGAGAGGT CCCTGGAGTT CGAGGACGGC GGCTCCGCCT CCGTGAGCGC CCACATCAGC
              481 CTGAGGGGCA ACACCTTCTA CCACAAGTCC AAGTTCACCG GCGTGAACTT CCCCGCCGAC
              541 GGCCCCATCA TGCAGAACCA GAGCGTGGAC TGGGAGCCCT CCACCGAGAA GATCACCGCC
              601 AGCGACGGCG TGCTGAAGGG CGACGTGACC ATGTACCTGA AGCTGGAGGG CGGCGGCAAC
              661 CACAAGTGCC AGTTCAAGAC CACCTACAAG GCCGCCAAGA AGATCCTGAA GATGCCCGGC
              721 AGCCACTACA TCAGCCACAG GCTGGTGAGG AAGACCGAGG GCAACATCAC CGAGCTGGTG
              781 GAGGACGCCG TGGCCCACTC CTAAGAATTC GAAGCTTGAT CCGGCTGCTA ACAAAGCCCG
              841 AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCCTTGGGG
              901 CCTCTAAACG GGTCTTGAGG GGTTTTTTTG CTGAAAGGAG GAACTATATC CGGGATCTGG
              961 CGTAATAGCG AAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTTGCGC AGCCCTGAAT
             1021 GGGCGAAATG GGACGCGCCC TGTAGCGGGC GCATTTAAGC CGGCGCGGGT TGTGATGGGT
             1081 TTACGCGCAG CCGTGAACCG GCTACACTTT GCCAGCCGAC CCCTAGGCGG GCCCGACATC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]

            2023.08.17

            GNP_filter3_RDBDEP_html_230702.pl