Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

hmKO1/pRSETB (#RDB15312)

Plasmid clone of fluorescent protein monomeric Kusabira-Orange (mKO1).

Clone info. Plasmid clone of fluorescent protein monomeric Kusabira-Orange (mKO1).
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15312 hmKO1/pRSETB DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The hmKO1/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15312).

Reference section:

Karasawa, S., Araki, T., Nagai, T., Mizuno, H., Miyawaki, A., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15312_A7H9p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp (Pr0261)
Region: 6xHis,T7 tag,Xpress™ tag,insert 5'
Sequence file: RDB15312_A7H9b.seq check
>D04498J3_A7H9_2_T7_20bp_C08_08_ABI24.ab1
    1 NNNNNNNNNN NNNNATTTTG TNNNNNTTAA GAAGGAGNNA TACATATGCG GGGTTCTCAT
   61 CATCATCATC ATCATGGTAT GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGGGATCTG
  121 TACGACGATG ACGATAAGGA TCCGACCATG GTGAGCGTGA TCAAGCCCGA GATGAAGATG
  181 AGGTACTACA TGGACGGCTC CGTCAATGGG CATGAGTTCA CAATCGAGGG TGAGGGCACA
  241 GGCAGACCTT ACGAGGGACA TCAGGAGATG ACACTGCGCG TCACAATGGC CAAGGGCGGG
  301 CCAATGCCTT TCGCCTTCGA CCTGGTGTCC CACGTGTTCT GTTACGGCCA CAGACCTTTT
  361 ACTAAATATC CAGAAGAGAT CCCAGACTAT TTCAAGCAGG CCTTTCCTGA GGGCCTGTCC
  421 TGGGAGAGGT CCCTGGAGTT CGAGGACGGC GGCTCCGCCT CCGTGAGCGC CCACATCAGC
  481 CTGAGGGGCA ACACCTTCTA CCACAAGTCC AAGTTCACCG GCGTGAACTT CCCCGCCGAC
  541 GGCCCCATCA TGCAGAACCA GAGCGTGGAC TGGGAGCCCT CCACCGAGAA GATCACCGCC
  601 AGCGACGGCG TGCTGAAGGG CGACGTGACC ATGTACCTGA AGCTGGAGGG CGGCGGCAAC
  661 CACAAGTGCC AGTTCAAGAC CACCTACAAG GCCGCCAAGA AGATCCTGAA GATGCCCGGC
  721 AGCCACTACA TCAGCCACAG GCTGGTGANG AAGANCGANG GCAACATCAC CGANCTGGTG
  781 GAGGANGCCG TGGCCCACTC CTAAGAATTC GAAGCTTGAT CCGGCTGCTA ACAAAGCCCG
  841 AAAGGAAGCT GANTTGGCTG CTGCCACCNC NNANCAATAA CNANCATAAC CCCCTTNGGG
  901 CCNNNNAACG GGNCTTGANG GGTTTTTTTG CTGAAAGGAN GAACTATNTN NNGNNTNNNG
961 NNNNNNNNCG NANNANNNCN GCNNNGNNNG CCNNNNNNNN NN
//
Primer: f1ori_R3 (Pr0142)
Region: T7 ter,insert 3'
Sequence file: RDB15312_A7H9c.seq check
>D04498J3_A7H9_2_f1ori_R3_D08_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNANN GTTGGGANGG CGNNCGGTGC GGGCCTCTTC GCTATTACGC
   61 CNGATCCGGA TATAGTTCCT CCTTTCAGCA AAAAACCCCT CAAGACCCGT TTAGAGGCCC
  121 CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC CAACTCAGCT TCCTTTCGGG
  181 CTTTGTTAGC AGCCGGATCA AGCTTCGAAT TCTTAGGAGT GGGCCACGGC GTCCTCCACC
  241 AGCTCGGTGA TGTTGCCCTC GGTCTTCCTC ACCAGCCTGT GGCTGATGTA GTGGCTGCCG
  301 GGCATCTTCA GGATCTTCTT GGCGGCCTTG TAGGTGGTCT TGAACTGGCA CTTGTGGTTG
  361 CCGCCGCCCT CCAGCTTCAG GTACATGGTC ACGTCGCCCT TCAGCACGCC GTCGCTGGCG
  421 GTGATCTTCT CGGTGGAGGG CTCCCAGTCC ACGCTCTGGT TCTGCATGAT GGGGCCGTCG
  481 GCGGGGAAGT TCACGCCGGT GAACTTGGAC TTGTGGTAGA AGGTGTTGCC CCTCAGGCTG
  541 ATGTGGGCGC TCACGGAGGC GGAGCCGCCG TCCTCGAACT CCAGGGACCT CTCCCAGGAC
  601 AGGCCCTCAG GAAAGGCCTG CTTGAAATAG TCTGGGATCT CTTCTGGATA TTTAGTAAAA
  661 GGTCTGTGGC CGTAACAGAA CACGTGGGAC ACCAGGTCGA AGGCGAAAGG CATTGGCCCG
  721 CCCTTGGCCA TTGTGACGCG CAGTGTCATC TCCTGATGTC CCTCGTAAGG TCTGCCTGTG
  781 CCCTCACCCT CGATTGTGAA CTCATGCCCA TTGACGGANC CGTCCATGTA GTACNNCATC
  841 TTCATCTCGG GCTTGATCAC GCTCACCNNG GNCGGNNCCT TATCGTCATC GNCGTACAGA
  901 TCCCGANCNN TTTGCTGNNC NCCANNCANG NNNGCCNTAC NNNGATGANG ANGATGATGA
961 GAACCNNNNN TATGNNNATN NNNNTNNNNN AANNNNANNN AANNTNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]