Resource data sheet
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mVenus-hGeminin(1/110) / pCSII-EF-MCS

Lentiviral expression vector of Fucci2-S/G2/M phase probe. Component of Fucci2 cell cycle probe.

Catalog number RDB15271
Resource name mVenus-hGeminin(1/110) / pCSII-EF-MCS
Clone info. Lentiviral expression vector of Fucci2-S/G2/M phase probe, S/G2/M-Y(N). One of the component of Fucci2 cell cycle probe. Fluorescence was detected in the nucleus.
For the composition, refer Fig.1 of Sakaue-Sawano, A., Chem. Biol. 15 (12): 1243-1248 (2008).
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Amp^r (E. coli), Zeo^r (mammalian cells)
Gene/insert name human Geminin cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence AB512479 (DDBJ accession number)
Originator Miyawaki, Atsushi |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248.
Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
(Japanese text [open/close])
Remarks ((Additional form)) Dr. Miyawaki's Approval
((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.
(Japanese text [open/close])

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15271_A7H3p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB15271_A7H3a.seq
>D04498I2-1_A7H3_EF1a-C_F_E01_13_ABI24.ab1
    1 AACCTCATGG GATTGCCGTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG
   61 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAAC ACGCTACCGG TCTCGAGAAT
  121 TCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG
  181 ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT
  241 ACGGCAAGCT GACCCTGAAG CTGATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA
  301 CCCTCGTGAC CACCCTGGGC TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA
  361 AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT
  421 TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC
  481 TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC
  541 ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA
  601 ACGGCATCAA GGCCAACTTC AAGATCCGCC ACAACATCGA GGACGGCGGC GTGCAGCTCG
  661 CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC
  721 ACTACCTGAG CTACCAGTCC AAGCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG
  781 TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAAG
  841 GGATATCCAT CACACTGGCG GCCGCTCGAG ATGAATCCCA GTATGAAGCA GAAACAAGAA
  901 GAAATCAAAG AGAATATAAA GAATAGTTCT GTCCCAAGAA GAACTCTGAA GATGATTCAG
  961 CCTTCTGCAT CTGGGATCTC TTGTTGGGAA GAGAAAATGA GCTGTCCGCA GGCTTGTCCC
 1021 AAAAGGAAAC ATCGGATGAC CACTTTAACA TCTACAACTT TCCAGCCCTG GGGGTTATTG
 1081 TCCCAGAATC TAGTGAAAAT AAAAATCTGG AGAGTCACCA GAAGTCAATT GGATCTTATG
1141 AATAAAGAAA AATCCATTCC CTCCTTCAGG TTATTTGGGA AAGGA
//
Primer: PRE_rev RDB15271_A7H3b.seq
>D04498I2-1_A7H3_PRE_rev_E02_14_ABI24.ab1
    1 ACCGTACCCA ATAGCGTAAA GGAGCAACAT AGTTAAGAAT ACCAGTCAAT CTTTCACAAA
   61 TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATCCC TCGATGTTAA CTCTAGATTA
  121 CAGCGCCTTT CTCCGTTTTT CTGCCACTTC CTTCCAATAC TGAGAGGATG GATTTTCTTT
  181 AATCATAAGA TCAAATGACT CCTGGGTGAC TCCTCCAAGA TTTTTATTTT CACTAGATTC
  241 TGGGACAATA ACCCCAGGGC TGGAAGTTGT AGATGTTAAG TGGTCATTCC GATGTTTCCT
  301 TTTGGACAAG CCTGCGGACA GCTCATTTTC TCTTCCAACA AGAGATCCAG ATGCAGAAGG
  361 CTGAATCATC TTCAGAGTTC TTCTTGGGAC AGAACTATTC TTTATATTCT CTTTGATTTC
  421 TTCTTGTTTC TGCTTCATAC TGGGATTCAT CTCGAGCGGC CGCCAGTGTG ATGGATATCC
  481 CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT CCAGCAGGAC
  541 CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGCTTG GACTGGTAGC TCAGGTAGTG
  601 GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT AGTGGTCGGC
  661 GAGCTGCACG CCGCCGTCCT CGATGTTGTG GCGGATCTTG AAGTTGGCCT TGATGCCGTT
  721 CTTCTGCTTG TCGGCGGTGA TATAGACGTT GTGGCTGTTG TAGTTGTACT CCAGCTTGTG
  781 CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC AGCTCGATGC GGTTCACCAG
  841 GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA
  901 GATGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC TTGAAGAAGT CGTGCTGCTT
  961 CATGTGGTCG GGGTAGCGGG CGAAGCACTG CAGCCGTAGC CCAGGTGGTC ACGAGGTGGG
 1021 CCAGGGCACG GGCAGCTTGC CGTGTGCAGA TCAGCTTCAG GTCAGCTGCC GTAGGTGGCA
 1081 TCGTCTCGTC TCGCGAACAC GCTGAACTGT GGCCGGTTAC GTCGCGGTCC AGCTCGGACC
1141 AGCATGGCAC CCACCCCGGG TGAACAAGCT TCCTCTCGG
//
Primer: CMV-Forward RDB15271_A7H3c.seq
>D04498I2-1_A7H3_CMV-Forward_E03_15_ABI24.ab1
    1 TTGTCGCTCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT
  121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG
  181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT
  241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC
  301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG
  361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG
  421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT
  481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT
  541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT
  601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC
  661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT
  721 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG GGCCGCTGAT
  781 CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA
  841 AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAG GCAAAGAGAA GAGTGGTGCA
  901 GAGAGAAAAA AGAGCAGTGG GAATAGGAGC TTTGTTCCTT GGGTTCTTGG GAGCAGCAGG
  961 AAGCACTATG GGCGCAGCGT CAATGACGCT GACGGTACAG GCCAGACAAT TATTGTCTGG
 1021 TATAGTGCAG CAGCAGAACA TTTTGCTGAG GGCTATTGAG CGCAACAGCC ATCCTGGTGC
 1081 CACTCACAGT CTGGGGCAAT CAAGCAGCTC AGCAGATCTG CTGGTGGAAG ATACCTAAAA
1141 GG
//
Primer: BGH_rev2 RDB15271_A7H3d.seq
>D04498I2-1_A7H3_BGH_rev2_E04_13_ABI24.ab1
    1 CCTTTCAACC GTTCAGTAGC ACGGGGGAGG GGCAACAAGC AGATGGCTGG CAGACTAGAA
   61 GGCACAGTCG AGGCTGATCA GCGGGTTTAA ACGGGCCCTG CTAGAGATTT TCCACACTGA
  121 CTAAAAGGGT CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC
  181 TTGAAGCACT CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA
  241 GAGCTCCCAG GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGTCCG GATGCAGCTC
  301 TCGGGCCATG TGATGAAATG CTAGGCGGCT GTCAAACCTC CACTCTAATA CTTCTCTCTC
  361 CGGGTCATCC ATCCCATGCA GGCTCACAGG GTGTAACAAG CGGGTGTTCT CTCCTTCATT
  421 GGCTTCTTCT ACCTTCTCTT GCTCAACTGG TACTAGCTTG TAGCACCATC CAAAGGTCAG
  481 TGGATATCTG ATCCCTGGCC CTGGTGTGTA GTTCTGCCAA TCAGGGAAGT AGCCTTGTGT
  541 GTGGTAGATC CACAGATCAA GGATATCTTG TCTTCGTTGG GAGTGAATTA GCCCTTCCAG
  601 TCCCCCCTTT TCTTTTAAAA AGTGGCTAAG ATCTACAGCT GCCTTGTAAG TCATTGGTCT
  661 TAAAGGTACC TGAGGTGTGA CTGGAAAACC CACCTCCTCC TCCTCTTGTG CTTCTAGCCA
  721 GGCACAATCA GCATTGGTAG CTGCTGTATT GCTACTTGTG ATTGCTCCAT GTTTTTCTAG
  781 GTCTCGACGG TATCGATGCG GGGAGGCGGC CCAAAGGGAG ATCCGACTCG TCTGAGGGCG
  841 AAGGCGAAGA CGCGGAAGAG GCCGCAGAGC CGGCAGCAGG CCGCGGGAAG GAAAGGTCCG
  901 CTGGATTGAG GGCCGAAAGG GACGTAGCAG AAGGACGTCC CGCGCAGAAT CCAGGTGGCA
  961 ACACAGGCGA GCAGCCATGG AAAGGACGTC AGCTTCCCCG ACACACCACG GAAATTGTCA
 1021 GTGCCAACAG CCGAGCCCCT GTCCAGCAGC GGGCAAGCAG CGGCGATGAG TTCCGCCGTT
 1081 GTATTAGGGA GGGGGGAAAG CGAAAGTCCT GCAAGAGCTT GACAGGTGAT GCATGCCCAA
1141 CATGGATGCC GTCAGCTAAC CCACAAAGT
//
Primer: SV40pro_F RDB15271_A7H3e.seq
>D04498I2-1_A7H3_SV40pro_F_E05_14_ABI24.ab1
    1 CCTGGTCCCT GGAACTTTCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
   61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT
  121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG
  181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT
  241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG
  301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA
  361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG
  421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT
  481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG
  541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG
  601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
  661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
  721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
  781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG
  841 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA
  901 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA
  961 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCCTGTC GTGCCAGCTG
 1021 CATTTAATGA ATCGGGCCCA ACGCGCGGGG GAGAGGCGGT TTGCGTATTG GGGCGCTTCT
 1081 TCGCTTCTCG CTCACTGACT CGCTTGCGCT CGATCGATCC GGCTGCCGGC GAGCGTATCA
1141 GGCTCACTCT CAAGACGATA CTACGGTTAT TACATCCTAT AG
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References

original Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression [Japanese text]. The journal of Japanese Biochemical Society (Seikagaku) 84 (1): 47-52 (2012).
original Sakaue-Sawano, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468.
reference Ohgushi, M., A RHO Small GTPase Regulator ABR Secures Mitotic Fidelity in Human Embryonic Stem Cells. Stem Cell Reports 9 (1): 58-66 (2017). PMID 28579391.
reference Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130.
reference Oki, T., A novel cell-cycle-indicator, mVenus-p27K-, identifies quiescent cells and visualizes G0-G1 transition. Sci. Rep. 4: 4012 (2014). PMID 24500246.
reference Abe, T., Visualization of cell cycle in mouse embryos with Fucci2 reporter directed by Rosa26 promoter. Development 140 (1): 237-246 (2013). PMID 23175634.

2017.10.26

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