Neuropeptide Y (NPY)-tagged fluorescent timer protein, monomeric Kusabira Green Orange (mK-GO).
Clone info. | Neuropeptide Y (NPY)-tagged fluorescent timer protein, monomeric Kusabira Green Orange (mK-GO). Green, yellow, and red fluores-cence of dense-core vesicle in PC12 cells were implicated as new, intermediate, and old ages, respectively (Tsuboi, T., Mol. Biol. Cell 21 (1): 87-94 (2010)). |
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Vector backbone | pCDNA3.1 (+) (Plasmid) |
Gene/insert name | Human NPY cDNA Fungia concinna Orange fluorescent protein cDNA |
Depositor|Developer | Miyawaki, Atsushi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, Miyawaki A. (2010) Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell 21 (1): 87-94. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. |
Remarks | Please obtain written approval of Dr. Atsushi Miyawaki. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, Miyawaki A. (2010) Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell 21 (1): 87-94. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. |
備考 | 提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15245 | NPY-mK-GO/pcDNA3.1(+) | DNA solution |
Materials & Methods section:
The NPY-mK-GO/pcDNA3.1(+) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15245). |
Reference section:
Tsuboi, T., Kitaguchi, T., Karasawa, S., Fukuda, M., Miyawaki, A., Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell. 21 (1): 87-94 (2010). PMID 19889833. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15245_A7H9p1-2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV_Forward (Pr0016) Region: CMV pro,T7 pro,insert 5'(NPY,mK-GO 5') Sequence file: RDB15245_A7H9g.seq |
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>D04498F3-2_A7H9_CMV-Forward_D08_11_ABI24.ab1 1 NNNNNNNNNN NNNTTGNNGC NNTGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC 61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT 121 CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACTTAA GCTTAGCCAC CATGCTAGGT 181 AACAAGCGAC TGGGGCTGTC CGGACTGACC CTCGCCCTGT CCCTGCTCGT GTGCCTGGGT 241 GCGCTGGCCG AGGCGTACCC CTCCAAGCCG GACAACCCGG GCGAGGACGC ACCAGCGGAG 301 GACATGGCCA GATACTACTC GGCGCTGCGA CACTACATCA ACCTCATCAC CAGGCAGAGA 361 TATGGAAAAC GATCCAGCCC AGAGACACTG ATTTCAGACC TCTTGATGAG AGAAAGCACA 421 GAAAATGTTC CCAGAACTCG GCTTGAAGAC CCTGCAATGT GGACGGTACC GCGGGCCCGG 481 GATCCGATGG TGAGTGTGAT TAAACCAGAG ATGAAGATGA GGTACTACAT GGACGGCTCC 541 GTCAATGGGC ATGAGTTCAC AATTGAAGGT GAAGGCACAG GCAGACCTTA CGAGGGACAT 601 CAAGAGATGA CACTACGCGT CACAATGGCC GAGGGCGGGC CAATGCCTTT CGCGTTTGAC 661 TTAGTGTCAC ACGTGTTCTG TTACGGCCAC AGAGTATTTA CTAAATATCC AGAAGAGATA 721 CCAGACTATT TCAAACAAGC ATTTCCTGAA NGCCTGTCAT GGGAAAGGTC GTTGGAGTTC 781 GAAGANGGTG GGTCCGCTTC AGTCAGTGCG CATATAAGCC TTAGAGGAAA CACCTTCTAC 841 CACNAATCCN AATTTACTGG GGTTNANTTT CCTGNNNATN GNCCTATCAT GNNAAANCNA 901 AGNGTTGNNT GGGANCNNTC AACCNANNAA NNTACTGNNN NNNNNNGNNT NCTNNANNNN 961 NGATNNTNNN NNNNNNN // |
Primer: bGH_rev2 (Pr0606) Region: bGHpA,insert 3'(mK-GO) Sequence file: RDB15245_A7H9h.seq |
>D04498F3-2_A7H9_BGH_rev2_E08_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGNGGNNGN NNNNNNNNNN GATGGCTGGC AACTAGAAGG 61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTCTA GACTCGAGCG GCCGCCACTG 121 TGCTGGATAT CTGCAGAATT CTTAGGAATG AGCTACTGCA TCTTCTACCA GCTCAGTAAT 181 GTTGCCTTCG GTTTTCCTGA CGAGGCGATG GCCGATGTAA TGGTCTCCTG GCATTTCAAG 241 AATCTCTTTT GCCGCCTTGT AAGTAGTCTT GAATTGGCAT TTGTGATTGC CGCCTCCTTC 301 AAGTTTTAGG TACATCGTAA CATCACCCTT CAGAACTCCG TCGCTGGCAG TAATTTTCTC 361 GGTTGATGGC TCCCAATCAA CACTTTGGTT TTGCATGATA GGACCATCGG CAGGAAAGTT 421 AACCCCAGTA AATTTGGATT TGTGGTAGAA GGTGTTTCCT CTAAGGCTTA TATGCGCACT 481 GACTGAAGCG GACCCACCAT CTTCGAACTC CAACGACCTT TCCCATGACA GGCCTTCAGG 541 AAATGCTTGT TTGAAATAGT CTGGTATCTC TTCTGGATAT TTAGTAAATA CTCTGTGGCC 601 GTAACAGAAC ACGTGTGACA CTAAGTCAAA CGCGAAAGGC ATTGGCCCGC CCTCGGCCAT 661 TGTGACGCGT AGTGTCATCT CTTGATGTCC CTCGTAAGGT CTGCCTGTGC CTTCACCTTC 721 AATTGTGAAC TCATGCCCAT TGACGGANCC GTCCATGTAG TACCTCATCT TCATCTCTGG 781 TTTAATCACA CTCACCATCG GATCCCGGGC CCGCGGTACC GTCCACATTG CANGGNCTTC 841 NAGCCNAGTT CTGGGAACAT TTTCTGTGCT TTCTCTCATC NAGANGNCTG AAATCAGTGT 901 CTCTGGGCTG GATNGNTTTN NNTATCTCTG CNNGGNNNNN ANNNNGANGN ANNNNCNCAN 961 CGCCNANNAN TATCNNGNNN NTNNNNNNNN NCNNNNNNNN NN // |
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR Sequence file: RDB15245_A7H9i.seq |
>D04498F3-2_A7H9_SV40pro_F_F08_17_ABI24.ab1 1 CNNNNNNNNN NNNNNNNNNN NNNNGTGNNG NNGNTTTTTT GGNNGCCTAG GCTTTTGCAA 61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC 121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG 181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG 241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA 301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC 421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA 481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA 541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT 601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT 661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT 721 GGAAAATGGC CGCTTTTCTG GANTCATCGA CTGTGNNCGG CTGGGTGTGG CGGANCGCTA 781 TCAGGACATA NCGTTGGCTA CNCGTGATAT TGCTGAAGAN CTTGGCGGCG AANGGGCTGA 841 NCGCTTCCTC GNGCTTTACN GTATCGCCGC TCCCGATTCG CAGCGCATCG CCNTCTATCG 901 CCTTCTTGNN GAGTNCTTNN NNNCGGGNNT NNNGGGGNTC NAAATGNNCG ANNNANNGAC 961 NCCNNANCNG NNNTCNCNNN NNTNNNNNNN NNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Tsuboi, T., Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell. 21 (1): 87-94 (2010). PMID 19889833. [link to RRC of NBRP] |
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