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NPY-mK-GO/pcDNA3.1(+) (#RDB15245)

Neuropeptide Y (NPY)-tagged fluorescent timer protein, monomeric Kusabira Green Orange (mK-GO).

Clone info. Neuropeptide Y (NPY)-tagged fluorescent timer protein, monomeric Kusabira Green Orange (mK-GO). Green, yellow, and red fluores-cence of dense-core vesicle in PC12 cells were implicated as new, intermediate, and old ages, respectively (Tsuboi, T., Mol. Biol. Cell 21 (1): 87-94 (2010)).
Vector backbone pCDNA3.1 (+) (Plasmid)
Gene/insert name Human NPY cDNA Fungia concinna Orange fluorescent protein cDNA
Depositor|Developer Miyawaki, Atsushi |
 
Sequence (full) RDB15245hts01.seq checkAssembled from experimentally sequenced data.
Publication Tsuboi, T., Mol. Biol. Cell. 21 (1): 87-94 (2010). [link to RRC of NBRP]
Test sheet RDB15245_B1Kmp1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Tsuboi, T. et al., Mol. Biol. Cell 21 (1): 87-94 (2010).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Tsuboi, T. et al., Mol. Biol. Cell 21 (1): 87-94 (2010).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15245 NPY-mK-GO/pcDNA3.1(+) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The NPY-mK-GO/pcDNA3.1(+) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15245).

Reference section:

Tsuboi, T., Kitaguchi, T., Karasawa, S., Fukuda, M., Miyawaki, A., Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell. 21 (1): 87-94 (2010). PMID 19889833. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Tsuboi, T., Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell. 21 (1): 87-94 (2010). PMID 19889833. [link to RRC of NBRP]