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NPY-mK-GO/pcDNA3.1(+) (#RDB15245)

Neuropeptide Y (NPY)-tagged fluorescent timer protein, monomeric Kusabira Green Orange (mK-GO).

Clone info. Neuropeptide Y (NPY)-tagged fluorescent timer protein, monomeric Kusabira Green Orange (mK-GO). Green, yellow, and red fluores-cence of dense-core vesicle in PC12 cells were implicated as new, intermediate, and old ages, respectively (Tsuboi, T., Mol. Biol. Cell 21 (1): 87-94 (2010)).
Vector backbone pCDNA3.1 (+) (Plasmid)
Gene/insert name Human NPY cDNA Fungia concinna Orange fluorescent protein cDNA
Depositor|Developer Miyawaki, Atsushi |
 

Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, Miyawaki A. (2010) Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell 21 (1): 87-94.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
宮脇敦史博士による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, Miyawaki A. (2010) Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell 21 (1): 87-94.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
備考 提供条件は英文のまま記載してください。
宮脇敦史博士の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15245 NPY-mK-GO/pcDNA3.1(+) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The NPY-mK-GO/pcDNA3.1(+) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15245).

Reference section:

Tsuboi, T., Kitaguchi, T., Karasawa, S., Fukuda, M., Miyawaki, A., Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell. 21 (1): 87-94 (2010). PMID 19889833. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15245_A7H9p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,T7 pro,insert 5'(NPY,mK-GO 5')
Sequence file: RDB15245_A7H9g.seq check
>D04498F3-2_A7H9_CMV-Forward_D08_11_ABI24.ab1
    1 NNNNNNNNNN NNNTTGNNGC NNTGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACTTAA GCTTAGCCAC CATGCTAGGT
  181 AACAAGCGAC TGGGGCTGTC CGGACTGACC CTCGCCCTGT CCCTGCTCGT GTGCCTGGGT
  241 GCGCTGGCCG AGGCGTACCC CTCCAAGCCG GACAACCCGG GCGAGGACGC ACCAGCGGAG
  301 GACATGGCCA GATACTACTC GGCGCTGCGA CACTACATCA ACCTCATCAC CAGGCAGAGA
  361 TATGGAAAAC GATCCAGCCC AGAGACACTG ATTTCAGACC TCTTGATGAG AGAAAGCACA
  421 GAAAATGTTC CCAGAACTCG GCTTGAAGAC CCTGCAATGT GGACGGTACC GCGGGCCCGG
  481 GATCCGATGG TGAGTGTGAT TAAACCAGAG ATGAAGATGA GGTACTACAT GGACGGCTCC
  541 GTCAATGGGC ATGAGTTCAC AATTGAAGGT GAAGGCACAG GCAGACCTTA CGAGGGACAT
  601 CAAGAGATGA CACTACGCGT CACAATGGCC GAGGGCGGGC CAATGCCTTT CGCGTTTGAC
  661 TTAGTGTCAC ACGTGTTCTG TTACGGCCAC AGAGTATTTA CTAAATATCC AGAAGAGATA
  721 CCAGACTATT TCAAACAAGC ATTTCCTGAA NGCCTGTCAT GGGAAAGGTC GTTGGAGTTC
  781 GAAGANGGTG GGTCCGCTTC AGTCAGTGCG CATATAAGCC TTAGAGGAAA CACCTTCTAC
  841 CACNAATCCN AATTTACTGG GGTTNANTTT CCTGNNNATN GNCCTATCAT GNNAAANCNA
  901 AGNGTTGNNT GGGANCNNTC AACCNANNAA NNTACTGNNN NNNNNNGNNT NCTNNANNNN
961 NGATNNTNNN NNNNNNN
//
Primer: bGH_rev2 (Pr0606)
Region: bGHpA,insert 3'(mK-GO)
Sequence file: RDB15245_A7H9h.seq check
>D04498F3-2_A7H9_BGH_rev2_E08_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGNGGNNGN NNNNNNNNNN GATGGCTGGC AACTAGAAGG
   61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTCTA GACTCGAGCG GCCGCCACTG
  121 TGCTGGATAT CTGCAGAATT CTTAGGAATG AGCTACTGCA TCTTCTACCA GCTCAGTAAT
  181 GTTGCCTTCG GTTTTCCTGA CGAGGCGATG GCCGATGTAA TGGTCTCCTG GCATTTCAAG
  241 AATCTCTTTT GCCGCCTTGT AAGTAGTCTT GAATTGGCAT TTGTGATTGC CGCCTCCTTC
  301 AAGTTTTAGG TACATCGTAA CATCACCCTT CAGAACTCCG TCGCTGGCAG TAATTTTCTC
  361 GGTTGATGGC TCCCAATCAA CACTTTGGTT TTGCATGATA GGACCATCGG CAGGAAAGTT
  421 AACCCCAGTA AATTTGGATT TGTGGTAGAA GGTGTTTCCT CTAAGGCTTA TATGCGCACT
  481 GACTGAAGCG GACCCACCAT CTTCGAACTC CAACGACCTT TCCCATGACA GGCCTTCAGG
  541 AAATGCTTGT TTGAAATAGT CTGGTATCTC TTCTGGATAT TTAGTAAATA CTCTGTGGCC
  601 GTAACAGAAC ACGTGTGACA CTAAGTCAAA CGCGAAAGGC ATTGGCCCGC CCTCGGCCAT
  661 TGTGACGCGT AGTGTCATCT CTTGATGTCC CTCGTAAGGT CTGCCTGTGC CTTCACCTTC
  721 AATTGTGAAC TCATGCCCAT TGACGGANCC GTCCATGTAG TACCTCATCT TCATCTCTGG
  781 TTTAATCACA CTCACCATCG GATCCCGGGC CCGCGGTACC GTCCACATTG CANGGNCTTC
  841 NAGCCNAGTT CTGGGAACAT TTTCTGTGCT TTCTCTCATC NAGANGNCTG AAATCAGTGT
  901 CTCTGGGCTG GATNGNTTTN NNTATCTCTG CNNGGNNNNN ANNNNGANGN ANNNNCNCAN
961 CGCCNANNAN TATCNNGNNN NTNNNNNNNN NCNNNNNNNN NN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR
Sequence file: RDB15245_A7H9i.seq check
>D04498F3-2_A7H9_SV40pro_F_F08_17_ABI24.ab1
    1 CNNNNNNNNN NNNNNNNNNN NNNNGTGNNG NNGNTTTTTT GGNNGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GANTCATCGA CTGTGNNCGG CTGGGTGTGG CGGANCGCTA
  781 TCAGGACATA NCGTTGGCTA CNCGTGATAT TGCTGAAGAN CTTGGCGGCG AANGGGCTGA
  841 NCGCTTCCTC GNGCTTTACN GTATCGCCGC TCCCGATTCG CAGCGCATCG CCNTCTATCG
  901 CCTTCTTGNN GAGTNCTTNN NNNCGGGNNT NNNGGGGNTC NAAATGNNCG ANNNANNGAC
961 NCCNNANCNG NNNTCNCNNN NNTNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Tsuboi, T., Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell. 21 (1): 87-94 (2010). PMID 19889833. [link to RRC of NBRP]