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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

NPY-mK-GO/pcDNA3.1(+)

Neuropeptide Y (NPY)-tagged fluorescent timer protein, monomeric Kusabira Green Orange (mK-GO).

Catalog number RDB15245
Resource name NPY-mK-GO/pcDNA3.1(+)
Clone info. Neuropeptide Y (NPY)-tagged fluorescent timer protein, monomeric Kusabira Green Orange (mK-GO). Green, yellow, and red fluores-cence of dense-core vesicle in PC12 cells were implicated as new, intermediate, and old ages, respectively (Tsuboi, T., Mol. Biol. Cell 21 (1): 87-94 (2010)).
Vector backbone pCDNA3.1 (+) (Plasmid)
Gene/insert name Human NPY cDNA Fungia concinna Orange fluorescent protein cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank
External Database
Human NPY Fungia concinna Orange fluorescent protein
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, Miyawaki A. (2010) Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell 21 (1): 87-94.
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, Miyawaki A. (2010) Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell 21 (1): 87-94.
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          宮脇敦史博士による提供承諾書 [Word]
          備考 提供条件は英文のまま記載してください。
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15245 NPY-mK-GO/pcDNA3.1(+) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The NPY-mK-GO/pcDNA3.1(+) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15245).

          Reference section:

          Tsuboi, T., Kitaguchi, T., Karasawa, S., Fukuda, M., Miyawaki, A., Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell. 21 (1): 87-94 (2010). PMID 19889833.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content Visualization of Organelles (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15245_A7H9p1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,T7 pro,insert 5'(NPY,mK-GO 5')
          Sequence file: RDB15245_A7H9d.seq check
          1
          Primer: bGH_rev2 (Pr0606)
          Region: bGHpA,insert 3'(mK-GO)
          Sequence file: RDB15245_A7H9e.seq check
          1
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB15245_A7H9f.seq check
          1

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Tsuboi, T., Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell. 21 (1): 87-94 (2010). PMID 19889833.

          2023.05.01

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