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NPY-mK-GO/pcDNA3.1(+)

Neuropeptide Y (NPY)-tagged fluorescent timer protein, monomeric Kusabira Green Orange (mK-GO).

Catalog number RDB15245
Resource name NPY-mK-GO/pcDNA3.1(+)
Clone info. Neuropeptide Y (NPY)-tagged fluorescent timer protein, monomeric Kusabira Green Orange (mK-GO). Green, yellow, and red fluores-cence of dense-core vesicle in PC12 cells were implicated as new, intermediate, and old ages, respectively (Tsuboi, T., Mol. Biol. Cell 21 (1): 87-94 (2010)).
Vector backbone Plasmid
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, Miyawaki A. (2010) Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell 21 (1): 87-94.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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RDB15245 NPY-mK-GO/pcDNA3.1(+) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Tsuboi, T., Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell. 21 (1): 87-94 (2010). PMID 19889833.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Organelle marker (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15245_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: CMV pro, insert | Primer: CMV_Forward | Sequence: RDB15245_A7H9a.seq
>D04498F3-2_A7H9_CMV-Forward_D08_11_ABI24.ab1
    1 CTCACTCCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACTTAA GCTTAGCCAC CATGCTAGGT
  181 AACAAGCGAC TGGGGCTGTC CGGACTGACC CTCGCCCTGT CCCTGCTCGT GTGCCTGGGT
  241 GCGCTGGCCG AGGCGTACCC CTCCAAGCCG GACAACCCGG GCGAGGACGC ACCAGCGGAG
  301 GACATGGCCA GATACTACTC GGCGCTGCGA CACTACATCA ACCTCATCAC CAGGCAGAGA
  361 TATGGAAAAC GATCCAGCCC AGAGACACTG ATTTCAGACC TCTTGATGAG AGAAAGCACA
  421 GAAAATGTTC CCAGAACTCG GCTTGAAGAC CCTGCAATGT GGACGGTACC GCGGGCCCGG
  481 GATCCGATGG TGAGTGTGAT TAAACCAGAG ATGAAGATGA GGTACTACAT GGACGGCTCC
  541 GTCAATGGGC ATGAGTTCAC AATTGAAGGT GAAGGCACAG GCAGACCTTA CGAGGGACAT
  601 CAAGAGATGA CACTACGCGT CACAATGGCC GAGGGCGGGC CAATGCCTTT CGCGTTTGAC
  661 TTAGTGTCAC ACGTGTTCTG TTACGGCCAC AGAGTATTTA CTAAATATCC AGAAGAGATA
  721 CCAGACTATT TCAAACAAGC ATTTCCTGAA GGCCTGTCAT GGGAAAGGTC GTTGGAGTTC
  781 GAAGATGGTG GGTCCGCTTC AGTCAGTGCG CATATAAGCC TTAGAGGAAA CACCTTCTAC
  841 CACAAATCCA AATTTACTGG GGTTAACTTT CCTGCCGATG GTCCTATCAT GCAAAACCAA
  901 AGTGTTGATT GGGAGCCATC AACCGAGAAA ATTACTGCCA GCGACGGAGT TCTGAAAGGG
  961 TGATGTTTAC GATGTACCTA AAACTTGAAA GGAGGCGGCA ATCACAAATG CCAAATTCAA
 1021 GACTACTTTA CACGCGGGCA AAAGAGATCC TTGAAATGCC CAGGAGACCA CTACATCGGG
1081 TCATTCGGTC CTTCGTTCAG GAAAAACCCG GTAAGGCCAC
//
Region: BGH p(A), mK-GO | Primer: BGH_rev2 | Sequence: RDB15245_A7H9b.seq
>D04498F3-2_A7H9_BGH_rev2_E08_14_ABI24.ab1
    1 CATTCATGGG TTATGGGGCA CGGGGGAGGG GCAACAAGCA GATGGCTGGC AACTAGAAGG
   61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTCTA GACTCGAGCG GCCGCCACTG
  121 TGCTGGATAT CTGCAGAATT CTTAGGAATG AGCTACTGCA TCTTCTACCA GCTCAGTAAT
  181 GTTGCCTTCG GTTTTCCTGA CGAGGCGATG GCCGATGTAA TGGTCTCCTG GCATTTCAAG
  241 AATCTCTTTT GCCGCCTTGT AAGTAGTCTT GAATTGGCAT TTGTGATTGC CGCCTCCTTC
  301 AAGTTTTAGG TACATCGTAA CATCACCCTT CAGAACTCCG TCGCTGGCAG TAATTTTCTC
  361 GGTTGATGGC TCCCAATCAA CACTTTGGTT TTGCATGATA GGACCATCGG CAGGAAAGTT
  421 AACCCCAGTA AATTTGGATT TGTGGTAGAA GGTGTTTCCT CTAAGGCTTA TATGCGCACT
  481 GACTGAAGCG GACCCACCAT CTTCGAACTC CAACGACCTT TCCCATGACA GGCCTTCAGG
  541 AAATGCTTGT TTGAAATAGT CTGGTATCTC TTCTGGATAT TTAGTAAATA CTCTGTGGCC
  601 GTAACAGAAC ACGTGTGACA CTAAGTCAAA CGCGAAAGGC ATTGGCCCGC CCTCGGCCAT
  661 TGTGACGCGT AGTGTCATCT CTTGATGTCC CTCGTAAGGT CTGCCTGTGC CTTCACCTTC
  721 AATTGTGAAC TCATGCCCAT TGACGGAGCC GTCCATGTAG TACCTCATCT TCATCTCTGG
  781 TTTAATCACA CTCACCATCG GATCCCGGGC CCGCGGTACC GTCCACATTG CAGGGTCTTC
  841 AAGCCGAGTT CTGGGAACAT TTTCTGTGCT TTCTCTCATC AAGAGGTCTG AAATCAGTGT
  901 CTCTGGGCTG GATCGTTTTC CATATCTCTG CCTGGTGATG AGGTTGATGT AGTGTCGCAG
  961 CGCCGAGTAG TATCTGGGCC ATGTCCTCCC GCTGGTGCGT CCCTCGCCCG GGTTGTCCGG
 1021 GCTTGGAGGG GTACGCCCTC GGCCAGCGCA CCCCAGGCAC ACGAGCAGGG ACAGGCGAGG
1081 GGTCAGTCAG ACAGGCCCCA GGTCGCTTGC TACCTAGCAA TGGGTTGGCT AAAGCT
//
Region: SV40pro_ori, NeoR | Primer: SV40pro_F | Sequence: RDB15245_A7H9c.seq
>D04498F3-2_A7H9_SV40pro_F_F08_17_ABI24.ab1
    1 CCTGGTCCTG TGACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG ACCAAGCGAC
  961 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTTCTATGAA GGTTGGGCTT
 1021 CGGAAATCGT TTTTCGGGAC GCCGGCTGGG ATGATCTTCA GCCGCGGGGA TCTCATGCTT
1081 GCAGTCATCG ACCACCCCAC TGATTTATTG CCAGCCTTAA TAAATTG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Tsuboi, T., Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell. 21 (1): 87-94 (2010). PMID 19889833.

2019.09.26

GNP_filter3_RDBDEP_html_190816.pl