Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mKikGR/pRSETB

Photoswitchable green fluorescent protein, monomeric Kikume Green-Red (mKikGR).

Catalog number RDB15261
Resource name mKikGR/pRSETB
Alternative name mKikGR13.2/pRSETB
Clone info. Monomeric Kikume Green-Red (mKikGR). It can be switched into red fluorescence upon illumination with UV light.
Vector backbone pRSET B (Plasmid)
Selectable markers Amp^r
Gene/insert name Favia favus Kikume Green-Red (mKikGR) fluorescent protein cDNA
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Habuchi S, Tsutsui H, Kochaniak AB, Miyawaki A, van Oijen AM. (2008) mKikGR, a monomeric photoswitchable fluorescent protein. PLoS One 3 (12): e3944.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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Remarks
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15261 mKikGR/pRSETB DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Habuchi, S., mKikGR, a monomeric photoswitchable fluorescent protein. PLoS One 3 (12): e3944 (2008). PMID 19079591.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15261_A7H9p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp
Sequence file: RDB15261_A7H9a.seq check
>D04498H-1_A7H9_T7_20bp_A01_01_ABI08.ab1
    1 AATTTACCTT CTAGAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACAT ATGCGGGGTT
   61 CTCATCATCA TCATCATCAT GGTATGGCTA GCATGACTGG TGGACAGCAA ATGGGTCGGG
  121 ATCTGTACGA CGATGACGAT AAGGATCCCA TGAGTGTGAT TACATCAGAA ATGAAGATCG
  181 AGCTGCGTAT GGAAGGCTCT GTAAACGGGC ACAAGTTCGT GATTACAGGG AAAGGAAGTG
  241 GCAGACCTTA CGAGGGAACA CAGACTGTGG ACCTGACAGT CATAGAGGGC GGACCTCTTC
  301 CTTTTGCTTT CGATATCCTG ACAACAGCAT TCCATTACGG CAACCGGGTA TTTGTCGAAT
  361 ACCCAGAAGA AATAGTAGAC TACTTCAAGC AGTCGTTTCC TGAGGGTTAT TCTTGGGAAC
  421 GAAGCATGAG TTACGAAGAC GGGGGAATTT GCCTCGCCAC AAACAATATA ACGATGAAGA
  481 AAGACGGCAG CAACACTTTT GTCAATGAAA TTCGATTTGA TGGTACAAAC TTTCCTGCCA
  541 ATGGTCCAGT TATGCAGAGG AAGACCGTCA AATGGGAGCC ATCCACTGAG AAAATGTATG
  601 TGCGTGATGG AGTGCTGAAG GGTGATGTTG AAATGGCTCT GTTGCTTGAA GGAGGTGGCC
  661 ATTACCGATG TGACTTCAGA ACTACTTACA AAGCAAAGAA GGTTGTCCAG TTGCCAGACT
  721 ATCACTACGT GGATCATCAA ATGGAGATAA CAAGCCATGA CAAGGATTAC AACAAGGTTA
  781 AGGCGTATGA ACATGCTAAG GCTTATTCCG GGACCTACAG GGGCGCCAAG TATGAATTTG
  841 AAGCCTAACT CGAGATCTGC AGCTGGTACC ATGGAATTCG AAGCTTGATC CGGCTGCTAA
  901 CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC
  961 CCTTGGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGAGGGA CTATATCCGG
 1021 GATCTGGCCG TAATAGCGAG GAGCCCGCAC CGATCGTCCT TCCCAACAGT GGCGCAGCCT
1081 GTATGGTCGA TTGGTACGCG CCCTGATAAG CGGGCGGCCA TTTAA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Habuchi, S., mKikGR, a monomeric photoswitchable fluorescent protein. PLoS One 3 (12): e3944 (2008). PMID 19079591.

2019.12.08

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