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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mKikGR/pRSETB

Photoswitchable green fluorescent protein, monomeric Kikume Green-Red (mKikGR).

Catalog number RDB15261
Resource name mKikGR/pRSETB
Alternative name mKikGR13.2/pRSETB
Clone info. Monomeric Kikume Green-Red (mKikGR). It can be switched into red fluorescence upon illumination with UV light.
Vector backbone pRSET B (Plasmid)
Selectable markers Amp^r
Gene/insert name Favia favus Kikume Green-Red (mKikGR) fluorescent protein cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank
External Database
Favia favus Kikume Green-Red (mKikGR) fluorescent protein
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Habuchi S, Tsutsui H, Kochaniak AB, Miyawaki A, van Oijen AM. (2008) mKikGR, a monomeric photoswitchable fluorescent protein. PLoS One 3 (12): e3944.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form             [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Habuchi S, Tsutsui H, Kochaniak AB, Miyawaki A, van Oijen AM. (2008) mKikGR, a monomeric photoswitchable fluorescent protein. PLoS One 3 (12): e3944.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15261 mKikGR/pRSETB DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The mKikGR/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15261).

            Reference section:

            Habuchi, S., Tsutsui, H., Kochaniak, A.B., Miyawaki, A., van Oijen, A.M., mKikGR, a monomeric photoswitchable fluorescent protein. PLoS One 3 (12): e3944 (2008). PMID 19079591.

            Further references such as user reports and related articles (go to bottom)

            References and tips

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15261_A7H9p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: T7_20bp (Pr0261)
            Region: RBS,6XHis,T7 tag,Xpress tag,insert (mKikGR),T7 ter.
            Sequence file: RDB15261_A7H9a.seq check
            >D04498H-1_A7H9_T7_20bp_A01_01_ABI08.ab1
                1 AATTTACCTT CTAGAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACAT ATGCGGGGTT
               61 CTCATCATCA TCATCATCAT GGTATGGCTA GCATGACTGG TGGACAGCAA ATGGGTCGGG
              121 ATCTGTACGA CGATGACGAT AAGGATCCCA TGAGTGTGAT TACATCAGAA ATGAAGATCG
              181 AGCTGCGTAT GGAAGGCTCT GTAAACGGGC ACAAGTTCGT GATTACAGGG AAAGGAAGTG
              241 GCAGACCTTA CGAGGGAACA CAGACTGTGG ACCTGACAGT CATAGAGGGC GGACCTCTTC
              301 CTTTTGCTTT CGATATCCTG ACAACAGCAT TCCATTACGG CAACCGGGTA TTTGTCGAAT
              361 ACCCAGAAGA AATAGTAGAC TACTTCAAGC AGTCGTTTCC TGAGGGTTAT TCTTGGGAAC
              421 GAAGCATGAG TTACGAAGAC GGGGGAATTT GCCTCGCCAC AAACAATATA ACGATGAAGA
              481 AAGACGGCAG CAACACTTTT GTCAATGAAA TTCGATTTGA TGGTACAAAC TTTCCTGCCA
              541 ATGGTCCAGT TATGCAGAGG AAGACCGTCA AATGGGAGCC ATCCACTGAG AAAATGTATG
              601 TGCGTGATGG AGTGCTGAAG GGTGATGTTG AAATGGCTCT GTTGCTTGAA GGAGGTGGCC
              661 ATTACCGATG TGACTTCAGA ACTACTTACA AAGCAAAGAA GGTTGTCCAG TTGCCAGACT
              721 ATCACTACGT GGATCATCAA ATGGAGATAA CAAGCCATGA CAAGGATTAC AACAAGGTTA
              781 AGGCGTATGA ACATGCTAAG GCTTATTCCG GGACCTACAG GGGCGCCAAG TATGAATTTG
              841 AAGCCTAACT CGAGATCTGC AGCTGGTACC ATGGAATTCG AAGCTTGATC CGGCTGCTAA
              901 CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC
              961 CCTTGGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGAGGGA CTATATCCGG
             1021 GATCTGGCCG TAATAGCGAG GAGCCCGCAC CGATCGTCCT TCCCAACAGT GGCGCAGCCT
            1081 GTATGGTCGA TTGGTACGCG CCCTGATAAG CGGGCGGCCA TTTAA
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Habuchi, S., mKikGR, a monomeric photoswitchable fluorescent protein. PLoS One 3 (12): e3944 (2008). PMID 19079591.

            2023.05.01

            GNP_filter3_RDBDEP_html_230501.pl