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Dronpa2/pRSETB (#RDB15257)

Plasmid clone of Dronpa2.

Clone info. Plasmid clone of Dronpa2.
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |
 
Sequence (partial) RDB15257pta01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Ando, R. et al., Biophys. J. 92 (12): L97-99 (2007).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Ando, R. et al., Biophys. J. 92 (12): L97-99 (2007).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15257 Dronpa2/pRSETB DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Dronpa2/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15257).

Reference section:

Ando, R., Flors, C., Mizuno, H., Hofkens, J., Miyawaki, A., Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Biophys. J. 92 (12): L97-99 (2007). PMID 17384059. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15257_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724)
Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
Sequence file: RDB15257_A7H9a.seq check
>15257_B0L4F_2_lacIpro-B_F_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNCGNNNC GNNTCNGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA
   61 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG GATCTCGATC CCGCGAAATT
  121 AATACGACTC ACTATAGGGA GACCACAACG GTTTCCCTCT AGAAATAATT TTGTTTAACT
  181 TTAAGAAGGA GATATACATA TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG
  241 CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCCGAT
  301 GAGTGTGATT AAACCAGACA TGAAGATCAA GCTGCGTATG GAAGGCGCTG TAAATGGACA
  361 CCCGTTCGCG ATTGAAGGAG TTGGCCTTGG GAAGCCTTTC GAGGGAAAAC AGAGTATGGA
  421 CCTTAAAGTC AAAGAAGGCG GACCTCTGCC TTTCGCCTAT GACATCTTGA CAACTGTGTT
  481 CTGTTACGGC AACAGGGTAT TCGCCAAATA CCCAGAAAAT ATAGTAGACT ATTTCAAGCA
  541 GTCGTTTCCT GAGGGCTACT CTTGGGAACG AAGCATGAAT TACGAAGACG GGGGCATTTG
  601 TAACGCGACA AACGACATAA CCCTCCATGG TGACTGTTAT ATCTATGAAA TTCGATTTGA
  661 TGGTGTGAAC TTTCCTGCCA ATGGTCCAGT TATGCAGAAG AGGACTGTGA AATGGGAGCC
  721 ATCCACTGAG AAATTGTATG TGCGTGATGG AGTGCTGAAN GGTGATGTTA ACACGGCTCT
  781 GTCGCTTGAA NGANNTGGNC ATTACCGATG TGTCTTCNAA ACTACTTATA AAGCTAAGAN
  841 GGTTGTCCAG TTGCCAGANT ATCACTTTGT GGACCACCAC ATTGAGATTA AAAGCCACGA
  901 CAAAGANTAC NGTANTGNTN ATCTGCNTGA NCACGCCNAA NCGCATTCTG ANCTGNCNAN
  961 GNNNNNCNAG TAANNNNTCN AANCTNGNTN CNNNTGNTNN CNAAGCCNNA ANNGNNNNNN
1021 NNNN
//
Primer: T7ter_R1 (Pr0798)
Region: insert, Xpress tag, T7 tag, 6xHis
Sequence file: RDB15257_A7H9b.seq check
>15257_B0L4F_2_T7ter_R1_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN NGTNNNTGNT NNGCGGTGGC AGCAGCCAAC TCAGCTTCCT
   61 TTCGGGCTTT GTTAGCAGCC GGATCAAGCT TCGAATTCTT ACTTGGCCTG CCTCGGCAGC
  121 TCAGAATGCG CTTCGGCGTG CTCATGCAGA TTAACATTAC TGTAATCTTT GTCGTGGCTT
  181 TTAATCTCAA TGTGGTGGTC CACAAAGTGA TAGTCTGGCA ACTGGACAAC CCTCTTAGCT
  241 TTATAAGTAG TTTTGAAGAC ACATCGGTAA TGGCCACCTC CTTCAAGCGA CAGAGCCGTG
  301 TTAACATCAC CCTTCAGCAC TCCATCACGC ACATACAATT TCTCAGTGGA TGGCTCCCAT
  361 TTCACAGTCC TCTTCTGCAT AACTGGACCA TTGGCAGGAA AGTTCACACC ATCAAATCGA
  421 ATTTCATAGA TATAACAGTC ACCATGGAGG GTTATGTCGT TTGTCGCGTT ACAAATGCCC
  481 CCGTCTTCGT AATTCATGCT TCGTTCCCAA GAGTAGCCCT CAGGAAACGA CTGCTTGAAA
  541 TAGTCTACTA TATTTTCTGG GTATTTGGCG AATACCCTGT TGCCGTAACA GAACACAGTT
  601 GTCAAGATGT CATAGGCGAA AGGCAGAGGT CCGCCTTCTT TGACTTTAAG GTCCATACTC
  661 TGTTTTCCCT CGAAAGGCTT CCCAAGGCCA ACTCCTTCAA TCGCGAACGG GTGTCCATTT
  721 ACAGCGCCTT CCATACGCAG CTTGATCTTC ATGTCTGGTT TAATCACACT CATCGGATCC
  781 TTATCGTCAT CGTCGTACAG ATCCCGACCC ATTTGCTGTC CACCAGTCAT GCTAGCCATA
  841 CCATGATGAT GATGATGANG AGAACCCCGC ATATGTATAT CTCCTTCTTA AAGTTAAACA
  901 AAATTATTTC TAGAGGGAAA NCGTTGTGGN CTCCCNATAG TGAGNCGNAN TAATTTCGCG
  961 GGNNNNANNT CCTGCNTTAA TGAATCGNNC NNCGCGCGGG GANANGNGGN TNGNGNATTG
1021 GGNNNTNNNN NNNNTNNNNN GNNNANNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ando, R., Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Biophys. J. 92 (12): L97-99 (2007). PMID 17384059. [link to RRC of NBRP]