RDB15257 - Dronpa2/pRSETB

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

Dronpa2/pRSETB (#RDB15257)

Plasmid clone of Dronpa2.

Clone info.	Plasmid clone of Dronpa2.
Vector backbone	Plasmid
Depositor\|Developer	Miyawaki, Atsushi \|

Sequence (partial)	RDB15257pta01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Ando R, Flors C, Mizuno H, Hofkens J, Miyawaki A. (2007) Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Biophys. J. 92 (12): L97-99.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.

Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Ando R, Flors C, Mizuno H, Hofkens J, Miyawaki A. (2007) Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Biophys. J. 92 (12): L97-99. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Ando R, Flors C, Mizuno H, Hofkens J, Miyawaki A. (2007) Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Biophys. J. 92 (12): L97-99. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15257	Dronpa2/pRSETB	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Dronpa2/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15257).

Reference section:

Ando, R., Flors, C., Mizuno, H., Hofkens, J., Miyawaki, A., Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Biophys. J. 92 (12): L97-99 (2007). PMID 17384059. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15257_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724) Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5' Sequence file: RDB15257_A7H9a.seq
>15257_B0L4F_2_lacIpro-B_F_E03_15_ABI24.ab1 1 NNNNNNNNNN NNNNCGNNNC GNNTCNGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA 61 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG GATCTCGATC CCGCGAAATT 121 AATACGACTC ACTATAGGGA GACCACAACG GTTTCCCTCT AGAAATAATT TTGTTTAACT 181 TTAAGAAGGA GATATACATA TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG 241 CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCCGAT 301 GAGTGTGATT AAACCAGACA TGAAGATCAA GCTGCGTATG GAAGGCGCTG TAAATGGACA 361 CCCGTTCGCG ATTGAAGGAG TTGGCCTTGG GAAGCCTTTC GAGGGAAAAC AGAGTATGGA 421 CCTTAAAGTC AAAGAAGGCG GACCTCTGCC TTTCGCCTAT GACATCTTGA CAACTGTGTT 481 CTGTTACGGC AACAGGGTAT TCGCCAAATA CCCAGAAAAT ATAGTAGACT ATTTCAAGCA 541 GTCGTTTCCT GAGGGCTACT CTTGGGAACG AAGCATGAAT TACGAAGACG GGGGCATTTG 601 TAACGCGACA AACGACATAA CCCTCCATGG TGACTGTTAT ATCTATGAAA TTCGATTTGA 661 TGGTGTGAAC TTTCCTGCCA ATGGTCCAGT TATGCAGAAG AGGACTGTGA AATGGGAGCC 721 ATCCACTGAG AAATTGTATG TGCGTGATGG AGTGCTGAAN GGTGATGTTA ACACGGCTCT 781 GTCGCTTGAA NGANNTGGNC ATTACCGATG TGTCTTCNAA ACTACTTATA AAGCTAAGAN 841 GGTTGTCCAG TTGCCAGANT ATCACTTTGT GGACCACCAC ATTGAGATTA AAAGCCACGA 901 CAAAGANTAC NGTANTGNTN ATCTGCNTGA NCACGCCNAA NCGCATTCTG ANCTGNCNAN 961 GNNNNNCNAG TAANNNNTCN AANCTNGNTN CNNNTGNTNN CNAAGCCNNA ANNGNNNNNN 1021 NNNN //
Primer: T7ter_R1 (Pr0798) Region: insert, Xpress tag, T7 tag, 6xHis Sequence file: RDB15257_A7H9b.seq
>15257_B0L4F_2_T7ter_R1_F03_18_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGNN NGTNNNTGNT NNGCGGTGGC AGCAGCCAAC TCAGCTTCCT 61 TTCGGGCTTT GTTAGCAGCC GGATCAAGCT TCGAATTCTT ACTTGGCCTG CCTCGGCAGC 121 TCAGAATGCG CTTCGGCGTG CTCATGCAGA TTAACATTAC TGTAATCTTT GTCGTGGCTT 181 TTAATCTCAA TGTGGTGGTC CACAAAGTGA TAGTCTGGCA ACTGGACAAC CCTCTTAGCT 241 TTATAAGTAG TTTTGAAGAC ACATCGGTAA TGGCCACCTC CTTCAAGCGA CAGAGCCGTG 301 TTAACATCAC CCTTCAGCAC TCCATCACGC ACATACAATT TCTCAGTGGA TGGCTCCCAT 361 TTCACAGTCC TCTTCTGCAT AACTGGACCA TTGGCAGGAA AGTTCACACC ATCAAATCGA 421 ATTTCATAGA TATAACAGTC ACCATGGAGG GTTATGTCGT TTGTCGCGTT ACAAATGCCC 481 CCGTCTTCGT AATTCATGCT TCGTTCCCAA GAGTAGCCCT CAGGAAACGA CTGCTTGAAA 541 TAGTCTACTA TATTTTCTGG GTATTTGGCG AATACCCTGT TGCCGTAACA GAACACAGTT 601 GTCAAGATGT CATAGGCGAA AGGCAGAGGT CCGCCTTCTT TGACTTTAAG GTCCATACTC 661 TGTTTTCCCT CGAAAGGCTT CCCAAGGCCA ACTCCTTCAA TCGCGAACGG GTGTCCATTT 721 ACAGCGCCTT CCATACGCAG CTTGATCTTC ATGTCTGGTT TAATCACACT CATCGGATCC 781 TTATCGTCAT CGTCGTACAG ATCCCGACCC ATTTGCTGTC CACCAGTCAT GCTAGCCATA 841 CCATGATGAT GATGATGANG AGAACCCCGC ATATGTATAT CTCCTTCTTA AAGTTAAACA 901 AAATTATTTC TAGAGGGAAA NCGTTGTGGN CTCCCNATAG TGAGNCGNAN TAATTTCGCG 961 GGNNNNANNT CCTGCNTTAA TGAATCGNNC NNCGCGCGGG GANANGNGGN TNGNGNATTG 1021 GGNNNTNNNN NNNNTNNNNN GNNNANNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Ando, R., Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Biophys. J. 92 (12): L97-99 (2007). PMID 17384059. [link to RRC of NBRP]

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