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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Dronpa2/pRSETB

Plasmid clone of Dronpa2.

Catalog number RDB15257
Resource name Dronpa2/pRSETB
Clone info. Plasmid clone of Dronpa2.
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Ando R, Flors C, Mizuno H, Hofkens J, Miyawaki A. (2007) Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Biophys. J. 92 (12): L97-99.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15257 Dronpa2/pRSETB DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence (partial) compiled by us RDB15257pta01.seq

Original reference

original Ando, R., Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Biophys. J. 92 (12): L97-99 (2007). PMID 17384059.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15257_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F
Region: T7 pro, RBS, 6xHis, T7 tag, Xpress tag, insert 5'
Sequence file: RDB15257_A7H9a.seq check
>15257_B0L4F_2_lacIpro-B_F_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNCGNNNC GNNTCNGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA
   61 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG GATCTCGATC CCGCGAAATT
  121 AATACGACTC ACTATAGGGA GACCACAACG GTTTCCCTCT AGAAATAATT TTGTTTAACT
  181 TTAAGAAGGA GATATACATA TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG
  241 CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCCGAT
  301 GAGTGTGATT AAACCAGACA TGAAGATCAA GCTGCGTATG GAAGGCGCTG TAAATGGACA
  361 CCCGTTCGCG ATTGAAGGAG TTGGCCTTGG GAAGCCTTTC GAGGGAAAAC AGAGTATGGA
  421 CCTTAAAGTC AAAGAAGGCG GACCTCTGCC TTTCGCCTAT GACATCTTGA CAACTGTGTT
  481 CTGTTACGGC AACAGGGTAT TCGCCAAATA CCCAGAAAAT ATAGTAGACT ATTTCAAGCA
  541 GTCGTTTCCT GAGGGCTACT CTTGGGAACG AAGCATGAAT TACGAAGACG GGGGCATTTG
  601 TAACGCGACA AACGACATAA CCCTCCATGG TGACTGTTAT ATCTATGAAA TTCGATTTGA
  661 TGGTGTGAAC TTTCCTGCCA ATGGTCCAGT TATGCAGAAG AGGACTGTGA AATGGGAGCC
  721 ATCCACTGAG AAATTGTATG TGCGTGATGG AGTGCTGAAN GGTGATGTTA ACACGGCTCT
  781 GTCGCTTGAA NGANNTGGNC ATTACCGATG TGTCTTCNAA ACTACTTATA AAGCTAAGAN
  841 GGTTGTCCAG TTGCCAGANT ATCACTTTGT GGACCACCAC ATTGAGATTA AAAGCCACGA
  901 CAAAGANTAC NGTANTGNTN ATCTGCNTGA NCACGCCNAA NCGCATTCTG ANCTGNCNAN
  961 GNNNNNCNAG TAANNNNTCN AANCTNGNTN CNNNTGNTNN CNAAGCCNNA ANNGNNNNNN
1021 NNNN
//
Primer: T7ter_R1
Region: insert, Xpress tag, T7 tag, 6xHis
Sequence file: RDB15257_A7H9b.seq check
>15257_B0L4F_2_T7ter_R1_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN NGTNNNTGNT NNGCGGTGGC AGCAGCCAAC TCAGCTTCCT
   61 TTCGGGCTTT GTTAGCAGCC GGATCAAGCT TCGAATTCTT ACTTGGCCTG CCTCGGCAGC
  121 TCAGAATGCG CTTCGGCGTG CTCATGCAGA TTAACATTAC TGTAATCTTT GTCGTGGCTT
  181 TTAATCTCAA TGTGGTGGTC CACAAAGTGA TAGTCTGGCA ACTGGACAAC CCTCTTAGCT
  241 TTATAAGTAG TTTTGAAGAC ACATCGGTAA TGGCCACCTC CTTCAAGCGA CAGAGCCGTG
  301 TTAACATCAC CCTTCAGCAC TCCATCACGC ACATACAATT TCTCAGTGGA TGGCTCCCAT
  361 TTCACAGTCC TCTTCTGCAT AACTGGACCA TTGGCAGGAA AGTTCACACC ATCAAATCGA
  421 ATTTCATAGA TATAACAGTC ACCATGGAGG GTTATGTCGT TTGTCGCGTT ACAAATGCCC
  481 CCGTCTTCGT AATTCATGCT TCGTTCCCAA GAGTAGCCCT CAGGAAACGA CTGCTTGAAA
  541 TAGTCTACTA TATTTTCTGG GTATTTGGCG AATACCCTGT TGCCGTAACA GAACACAGTT
  601 GTCAAGATGT CATAGGCGAA AGGCAGAGGT CCGCCTTCTT TGACTTTAAG GTCCATACTC
  661 TGTTTTCCCT CGAAAGGCTT CCCAAGGCCA ACTCCTTCAA TCGCGAACGG GTGTCCATTT
  721 ACAGCGCCTT CCATACGCAG CTTGATCTTC ATGTCTGGTT TAATCACACT CATCGGATCC
  781 TTATCGTCAT CGTCGTACAG ATCCCGACCC ATTTGCTGTC CACCAGTCAT GCTAGCCATA
  841 CCATGATGAT GATGATGANG AGAACCCCGC ATATGTATAT CTCCTTCTTA AAGTTAAACA
  901 AAATTATTTC TAGAGGGAAA NCGTTGTGGN CTCCCNATAG TGAGNCGNAN TAATTTCGCG
  961 GGNNNNANNT CCTGCNTTAA TGAATCGNNC NNCGCGCGGG GANANGNGGN TNGNGNATTG
1021 GGNNNTNNNN NNNNTNNNNN GNNNANNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ando, R., Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Biophys. J. 92 (12): L97-99 (2007). PMID 17384059.

2021.02.18

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