Please review the QC test results indicated by icon below as well as clone information before placing your order.

tdStayGold/pcDNA3

Fluorescent protein tdStayGold for fusion to the C-terminus of target molecule

Sequence (full)	RDB19609zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

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提供条件	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19609_B2Eap1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5'((GGGGS)x3,tdStayGold 5') Sequence file: RDB19609_B2Eaa.seq
>D08836A5_B2Ea_3_CMV_Forward_C04_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNTG NNGCNNATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA    61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA   121 CGACTCACTA TAGGGAGACC CAAGCTTGGT ACCGCATGGT GGAGGCGGTT CAGGCGGAGG   181 TGGCTCTGGC GGTGGCGGAT CGATCGGGGA TCCGATGGTG TCTACAGGCG AGGAGCTGTT   241 TACCGGCGTG GTGCCCTTCA AGTTCCAGCT GAAGGGCACC ATCAACGGCA AGAGCTTCAC   301 CGTGGAAGGC GAGGGCGAGG GCAATAGCCA CGAGGGCAGC CACAAAGGCA AGTACGTGTG   361 CACCAGCGGC AAACTGCCAA TGTCTTGGGC CGCCCTGGGA ACTAGCTTCG GCTATGGCAT   421 GAAGTACTAC ACCAAGTACC CCAGCGGCCT GAAGAACTGG TTCCACGAGG TGATGCCCGA   481 GGGCTTCACC TACGACAGAC ACATCCAGTA CAAGGGCGAC GGCAGCATCC ACGCCAAGCA   541 CCAGCACTTC ATGAAGAACG GCACCTACCA CAACATCGTG GAGTTCACCG GCCAGGACTT   601 CAAGGAGAAC AGCCCCGTGC TGACCGGCGA CATGAACGTG AGCCTGCCCA ACGAGGTGCA   661 GCACATCCCC AGAGATGACG GCGTGGAGTG CCCAGTGACC CTGCTGTACC CTCTGCTGAG   721 CGACAAGAGC AAGTGCGTGG AGGCCCACCA GAACACCATC TGCAAGCCCC TGCACAATCA   781 GCCAGCCCCC GATGTGCCAT ACCACTGGAT CAGAAAGCAG TACACCCAGA GCAAGGACGA   841 NACCGANGAN AGAGANCACA TCTGCCANAN CGAGANCCTG GNNGCCCACC TGCCATGGCA   901 CGAGCCTTCN GCTTCTGNCG NGNAATTCAN NGNNNGTGGT TCTGCTNGAN GAANCGCCNN   961 NNNNTCNNCN GGGGGNAAGC NNNNNNNCNN CNGGNNGCNC TNGCNGNNNN GNNNGGGGGN 1021 ANTNNNNNNN NNGNNNNNNN NNN //
Primer: BGH_rev2 (Pr0606) Region: insert 3'(tdStayGold 3') Sequence file: RDB19609_B2Eab.seq
>D08836A5_B2Ea_3_BGH_rev2_C11_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGNNNNNNA CAGANGGCTG GCAACTAGAA    61 GGCACAGTCG AGGCTGNTCN NNNAGCTCTA GCATTTAGGT GACACTATAG AATAGGGCCC   121 TCTAGATGCA TGCTCGAGCG GCCGCTTATA AGTGTGCTTC GAGAGTCTCT GATTGACAAA   181 TATGATCACG TTCCTCGGTA TCATCTTTGC TTTGTGTGTA TTGTTTACGA ATCCAGTGAT   241 ATGGGACATC AGGTGCTGGT TGATTATGAA GAGGCTTGCA GATTGTATTT TGGTGAGCCT   301 CAACGCATTT TGATTTATCC GATAATAAAG GATAAAGCAA GGTCACTGGG CATTCTACTC   361 CATCATCTCT GGGTATATGT TGAACTTCAT TCGGTAATGA GACATTCATA TCTCCAGTTA   421 AGACTGGACT ATTTTCTTTA AAATCCTGAC CAGTAAATTC AACAATGTTG TGATAAGTCC   481 CATTTTTCAT AAAGTGTTGG TGTTTTGCAT GGATACTCCC ATCGCCTTTA TATTGAATAT   541 GACGATCGTA GGTAAAGCCT TCAGGCATTA CTTCATGAAA CCAGTTCTTC AGTCCACTAG   601 GATATTTGGT ATAATATTTC ATTCCATAAC CAAAGGATGT TCCAAGTGCT GCCCATGACA   661 TCGGTAGTTT TCCACTTGTA CAAACATATT TTCCTTTATG AGAACCTTCA TGTGAATTTC   721 CTTCACCTTC NCCTTCAACG GTAAACGATT TGCCATTGAT GGTTCCTTTA AGTTGAAATT   781 TAAATGGCAC CACCCCGGTG AACAGCTCCT CCCCTGTACT CNCCATAAGC TTCCCACCNG   841 CGCTACCGNC NGCACTCNCT CCNGCGGAAN CGCCAGCAGA NCCACNNNCA GATGTNGANC   901 CTCCANCGCN NCCACCNGCA CNNNNNGANC CCCCCGGNAG ATNCACNNGC GCTNCNNNNA 961 GNNGNANNNN CNTGCACNAC CCCNNAGCAN NNGTNNNTTN CNNNNNNNNN N //
Primer: hStayGold_F1 (Pr1213) Region: insert mid Sequence file: RDB19609_B2Eac.seq
>D08836A5_B2Ea_3_hStayGold_F1_D04_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNCCTGCC NNACGAGGTG CAGCACATCC CCAGAGATGA    61 NGGCGTGGAG TGCCCAGTGA CCCTGCTGTA CCCTCTGCTG AGCGACAAGA GCAAGTGCGT   121 GGAGGCCCAC CAGAACACCA TCTGCAAGCC CCTGCACAAT CAGCCAGCCC CCGATGTGCC   181 ATACCACTGG ATCAGAAAGC AGTACACCCA GAGCAAGGAC GACACCGAGG AGAGAGACCA   241 CATCTGCCAG AGCGAGACCC TGGAGGCCCA CCTGCCATGG CACGAGCCTT CTGCTTCTGC   301 CGTGGAATTC AGTGCTGGTG GTTCTGCTGG AGGAAGCGCC GGAGGTTCCG CTGGGGGAAG   361 CGCAGGCGGC TCCGGCAGCG CTGGCGGGAG TGCCGGGGGC AGTACCTCCG CAGGAGGAAG   421 TGCAGGGGGA AGCGCTGGTG GCAGTGCGGG GGGTTCCGCA GGCGGTAGCG GTTCCGCCGG   481 TGGATCTGCC GGTGGAAGCA CATCTGCTGG GGGTAGTGCA GGAGGGTCCG CTGGTGGGAG   541 CGCAGGTGGA TCTGCCGGGG GGTCTGGAAG TGCAGGTGGT AGCGCTGGAG GGTCCACATC   601 TGCAGGTGGG TCTGCTGGCG GTTCCGCCGG AGGGAGTGCC GGCGGTAGCG CCGGTGGGAA   661 GCTTATGGTG AGTACAGGGG AGGAGCTGTT CACCGGGGTG GTGCCATTTA AATTTCAACT   721 TAAAGGAACC ATCAATGGCA AATCGTTTAN CGTTGAAGGC GAANGTGAAG GAAATTCACA   781 TGAAGGTTCT CATAAAGGAA AATATGTTTG TACAAGTGGA AAACTACCGA TGTCATGGGC   841 AGCACTTGGA ACATCCTTTG GTTATGGAAT GAAATATTAT ACCAAATATC CNANTGNANT   901 GAANAACTGG NTTCATGAAG TAATGNCTGA AGGNTTTNNC TACNATCGTC NTATTCNATA   961 TAAANGNNAN GGGANTATNC NTGCAAAACA CCNACNCTTT ATGAAAAATG GNNNTNNTCA 1021 CNNNNTTGNT NNNNTTNCNN NNNNNNATTT NNNNNNNNNN //
Primer: EV_linker_F1 (Pr1214) Region: insert mid Sequence file: RDB19609_B2Ead.seq
>D08836A5_B2Ea_3_EV_linker_F1_E04_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG NNGGNNNGCN NGTGGNNCTG CCGGGGGGTC TGGAAGTGCA    61 GGTGGTAGCG CTGGAGGGTC CACATCTGCA GGTGGGTCTG CTGGCGGTTC CGCCGGAGGG   121 AGTGCCGGCG GTAGCGCCGG TGGGAAGCTT ATGGTGAGTA CAGGGGAGGA GCTGTTCACC   181 GGGGTGGTGC CATTTAAATT TCAACTTAAA GGAACCATCA ATGGCAAATC GTTTACCGTT   241 GAAGGCGAAG GTGAAGGAAA TTCACATGAA GGTTCTCATA AAGGAAAATA TGTTTGTACA   301 AGTGGAAAAC TACCGATGTC ATGGGCAGCA CTTGGAACAT CCTTTGGTTA TGGAATGAAA   361 TATTATACCA AATATCCTAG TGGACTGAAG AACTGGTTTC ATGAAGTAAT GCCTGAAGGC   421 TTTACCTACG ATCGTCATAT TCAATATAAA GGCGATGGGA GTATCCATGC AAAACACCAA   481 CACTTTATGA AAAATGGGAC TTATCACAAC ATTGTTGAAT TTACTGGTCA GGATTTTAAA   541 GAAAATAGTC CAGTCTTAAC TGGAGATATG AATGTCTCAT TACCGAATGA AGTTCAACAT   601 ATACCCAGAG ATGATGGAGT AGAATGCCCA GTGACCTTGC TTTATCCTTT ATTATCGGAT   661 AAATCAAAAT GCGTTGAGGC TCACCAAAAT ACAATCTGCA AGCCTCTTCA TAATCAACCA   721 GCACCTGATG TCCCATATCA CTGGATTCGT AAACAATACA CACAAAGCAN AGANGANACC   781 GANGAANGTG ATCATATTTG TCAATCANAN ANTCTCGAAN CACACTTATA ANCGGCCGCT   841 CGAGCATGCA TCTAGAGGGN CCTATTCTAT AGTGNCACCT AAATGNTANA NCTCGCTGAN   901 CANCCTCNAN TGTGCCTTCN ANTTGNCAGC CATCTGTTGN TTGNCCCNCC CCCNNGNCNT   961 NCNTGNNCCN NGNANNNGNC NNNCCNNCNG NNNNTTNNNN NTNAAATGNN NAAANNNNNN 1021 NNNANNNTCT GANNNNNNNN NN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR Sequence file: RDB19609_B2Eae.seq
>D08836A5_B2Ea_3_SV40pro_ori_F_G04_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNGNNN CCTCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT    61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC   121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG   181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG   241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA   301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT   361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA   421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC   481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC   541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC   601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT   661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA   721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG   781 CCGGCTGGGT GTGGCGGANC GCTATCANGA CATAGCGTTG GCTACCCGTG ATATTGCTGA   841 AGANCTTGGC GGCGAATGNN CTGACCGCTT CCTCGTGCTT TACNGTATCG CCGCTCCCGA   901 NTCGCANCGC ATCGCCTTCN ATCGCCTTCT TGANNANTNC TTCNGANCGG GNNTCTGGGG   961 NTCNAAATGA NCGANCAAGC GNNGCCCAAN CNNGNCNTCN CNANNNTTCN ANNNNNNNNN 1021 CNNNNNNCTA NGAAANNNNN GNNNTNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl