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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

tdStayGold/pcDNA3

Fluorescent protein tdStayGold for fusion to the C-terminus of target molecule

Catalog number RDB19609
Resource name tdStayGold/pcDNA3
Clone info. For fusion of tdStayGold to the C-terminus of a target molecule (X). tdStayGold is an intramolecular dimeric unit that performs as a monomer, and is composed of n1, StayGold, c4, EV linker, n1, and StayGold. The gene for the former StayGold has been resynthesized with mammalian-preferred codons, and the gene for the latter StayGold has the jellyfish original codons. n1 and c4 are the appendages that facilitate fusions to the N- and C-termini of StayGold, respectively. X-tdStayGold is required to terminate with the original C-terminus of StayGold. See Hirano, M. et al., Nat. Biotechnol. 2022 doi: 10.1038/s41587-022-01278-2 for more information.
Comment The accession numbers in the DDBJ/EMBL/GenBank databases are LC601652 for StayGold, LC593679 for h-StayGold and LC601653 for oxStayGold.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell).
Gene/insert name Cytaeis uchidae GFP cDNA
Depositor|Developer Miyawaki, Atsushi | Ando, Ryoko |
Other clones in our bank

External Database
Cytaeis uchidae GFP

            Reference sequence
              
             
            Sequence (full) RDB19609zzk02.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB19609 tdStayGold/pcDNA3 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Hirano, M., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 2022 Apr 25. doi: 10.1038/s41587-022-01278-2. Epub ahead of print. PMID 35468954.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content 明るく、退色しにくい新規の緑色蛍光タンパク質 「StayGold」 (Japanese text)
            Featured content StayGold, highly photostable GFP (English text)
            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB19609_B2Eap1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward
            Region: CMV pro,insert 5'((GGGGS)x3,tdStayGold 5')
            Sequence file: RDB19609_B2Eaa.seq check
            >D08836A5_B2Ea_3_CMV_Forward_C04_07_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNTG NNGCNNATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
               61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
              121 CGACTCACTA TAGGGAGACC CAAGCTTGGT ACCGCATGGT GGAGGCGGTT CAGGCGGAGG
              181 TGGCTCTGGC GGTGGCGGAT CGATCGGGGA TCCGATGGTG TCTACAGGCG AGGAGCTGTT
              241 TACCGGCGTG GTGCCCTTCA AGTTCCAGCT GAAGGGCACC ATCAACGGCA AGAGCTTCAC
              301 CGTGGAAGGC GAGGGCGAGG GCAATAGCCA CGAGGGCAGC CACAAAGGCA AGTACGTGTG
              361 CACCAGCGGC AAACTGCCAA TGTCTTGGGC CGCCCTGGGA ACTAGCTTCG GCTATGGCAT
              421 GAAGTACTAC ACCAAGTACC CCAGCGGCCT GAAGAACTGG TTCCACGAGG TGATGCCCGA
              481 GGGCTTCACC TACGACAGAC ACATCCAGTA CAAGGGCGAC GGCAGCATCC ACGCCAAGCA
              541 CCAGCACTTC ATGAAGAACG GCACCTACCA CAACATCGTG GAGTTCACCG GCCAGGACTT
              601 CAAGGAGAAC AGCCCCGTGC TGACCGGCGA CATGAACGTG AGCCTGCCCA ACGAGGTGCA
              661 GCACATCCCC AGAGATGACG GCGTGGAGTG CCCAGTGACC CTGCTGTACC CTCTGCTGAG
              721 CGACAAGAGC AAGTGCGTGG AGGCCCACCA GAACACCATC TGCAAGCCCC TGCACAATCA
              781 GCCAGCCCCC GATGTGCCAT ACCACTGGAT CAGAAAGCAG TACACCCAGA GCAAGGACGA
              841 NACCGANGAN AGAGANCACA TCTGCCANAN CGAGANCCTG GNNGCCCACC TGCCATGGCA
              901 CGAGCCTTCN GCTTCTGNCG NGNAATTCAN NGNNNGTGGT TCTGCTNGAN GAANCGCCNN
              961 NNNNTCNNCN GGGGGNAAGC NNNNNNNCNN CNGGNNGCNC TNGCNGNNNN GNNNGGGGGN
            1021 ANTNNNNNNN NNGNNNNNNN NNN
            //
            Primer: BGH_rev2
            Region: insert 3'(tdStayGold 3')
            Sequence file: RDB19609_B2Eab.seq check
            >D08836A5_B2Ea_3_BGH_rev2_C11_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGNNNNNNA CAGANGGCTG GCAACTAGAA
               61 GGCACAGTCG AGGCTGNTCN NNNAGCTCTA GCATTTAGGT GACACTATAG AATAGGGCCC
              121 TCTAGATGCA TGCTCGAGCG GCCGCTTATA AGTGTGCTTC GAGAGTCTCT GATTGACAAA
              181 TATGATCACG TTCCTCGGTA TCATCTTTGC TTTGTGTGTA TTGTTTACGA ATCCAGTGAT
              241 ATGGGACATC AGGTGCTGGT TGATTATGAA GAGGCTTGCA GATTGTATTT TGGTGAGCCT
              301 CAACGCATTT TGATTTATCC GATAATAAAG GATAAAGCAA GGTCACTGGG CATTCTACTC
              361 CATCATCTCT GGGTATATGT TGAACTTCAT TCGGTAATGA GACATTCATA TCTCCAGTTA
              421 AGACTGGACT ATTTTCTTTA AAATCCTGAC CAGTAAATTC AACAATGTTG TGATAAGTCC
              481 CATTTTTCAT AAAGTGTTGG TGTTTTGCAT GGATACTCCC ATCGCCTTTA TATTGAATAT
              541 GACGATCGTA GGTAAAGCCT TCAGGCATTA CTTCATGAAA CCAGTTCTTC AGTCCACTAG
              601 GATATTTGGT ATAATATTTC ATTCCATAAC CAAAGGATGT TCCAAGTGCT GCCCATGACA
              661 TCGGTAGTTT TCCACTTGTA CAAACATATT TTCCTTTATG AGAACCTTCA TGTGAATTTC
              721 CTTCACCTTC NCCTTCAACG GTAAACGATT TGCCATTGAT GGTTCCTTTA AGTTGAAATT
              781 TAAATGGCAC CACCCCGGTG AACAGCTCCT CCCCTGTACT CNCCATAAGC TTCCCACCNG
              841 CGCTACCGNC NGCACTCNCT CCNGCGGAAN CGCCAGCAGA NCCACNNNCA GATGTNGANC
              901 CTCCANCGCN NCCACCNGCA CNNNNNGANC CCCCCGGNAG ATNCACNNGC GCTNCNNNNA
            961 GNNGNANNNN CNTGCACNAC CCCNNAGCAN NNGTNNNTTN CNNNNNNNNN N
            //
            Primer: hStayGold_F1
            Region: insert mid
            Sequence file: RDB19609_B2Eac.seq check
            >D08836A5_B2Ea_3_hStayGold_F1_D04_10_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNCCTGCC NNACGAGGTG CAGCACATCC CCAGAGATGA
               61 NGGCGTGGAG TGCCCAGTGA CCCTGCTGTA CCCTCTGCTG AGCGACAAGA GCAAGTGCGT
              121 GGAGGCCCAC CAGAACACCA TCTGCAAGCC CCTGCACAAT CAGCCAGCCC CCGATGTGCC
              181 ATACCACTGG ATCAGAAAGC AGTACACCCA GAGCAAGGAC GACACCGAGG AGAGAGACCA
              241 CATCTGCCAG AGCGAGACCC TGGAGGCCCA CCTGCCATGG CACGAGCCTT CTGCTTCTGC
              301 CGTGGAATTC AGTGCTGGTG GTTCTGCTGG AGGAAGCGCC GGAGGTTCCG CTGGGGGAAG
              361 CGCAGGCGGC TCCGGCAGCG CTGGCGGGAG TGCCGGGGGC AGTACCTCCG CAGGAGGAAG
              421 TGCAGGGGGA AGCGCTGGTG GCAGTGCGGG GGGTTCCGCA GGCGGTAGCG GTTCCGCCGG
              481 TGGATCTGCC GGTGGAAGCA CATCTGCTGG GGGTAGTGCA GGAGGGTCCG CTGGTGGGAG
              541 CGCAGGTGGA TCTGCCGGGG GGTCTGGAAG TGCAGGTGGT AGCGCTGGAG GGTCCACATC
              601 TGCAGGTGGG TCTGCTGGCG GTTCCGCCGG AGGGAGTGCC GGCGGTAGCG CCGGTGGGAA
              661 GCTTATGGTG AGTACAGGGG AGGAGCTGTT CACCGGGGTG GTGCCATTTA AATTTCAACT
              721 TAAAGGAACC ATCAATGGCA AATCGTTTAN CGTTGAAGGC GAANGTGAAG GAAATTCACA
              781 TGAAGGTTCT CATAAAGGAA AATATGTTTG TACAAGTGGA AAACTACCGA TGTCATGGGC
              841 AGCACTTGGA ACATCCTTTG GTTATGGAAT GAAATATTAT ACCAAATATC CNANTGNANT
              901 GAANAACTGG NTTCATGAAG TAATGNCTGA AGGNTTTNNC TACNATCGTC NTATTCNATA
              961 TAAANGNNAN GGGANTATNC NTGCAAAACA CCNACNCTTT ATGAAAAATG GNNNTNNTCA
            1021 CNNNNTTGNT NNNNTTNCNN NNNNNNATTT NNNNNNNNNN
            //
            Primer: EV_linker_F1
            Region: insert mid
            Sequence file: RDB19609_B2Ead.seq check
            >D08836A5_B2Ea_3_EV_linker_F1_E04_13_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNG NNGGNNNGCN NGTGGNNCTG CCGGGGGGTC TGGAAGTGCA
               61 GGTGGTAGCG CTGGAGGGTC CACATCTGCA GGTGGGTCTG CTGGCGGTTC CGCCGGAGGG
              121 AGTGCCGGCG GTAGCGCCGG TGGGAAGCTT ATGGTGAGTA CAGGGGAGGA GCTGTTCACC
              181 GGGGTGGTGC CATTTAAATT TCAACTTAAA GGAACCATCA ATGGCAAATC GTTTACCGTT
              241 GAAGGCGAAG GTGAAGGAAA TTCACATGAA GGTTCTCATA AAGGAAAATA TGTTTGTACA
              301 AGTGGAAAAC TACCGATGTC ATGGGCAGCA CTTGGAACAT CCTTTGGTTA TGGAATGAAA
              361 TATTATACCA AATATCCTAG TGGACTGAAG AACTGGTTTC ATGAAGTAAT GCCTGAAGGC
              421 TTTACCTACG ATCGTCATAT TCAATATAAA GGCGATGGGA GTATCCATGC AAAACACCAA
              481 CACTTTATGA AAAATGGGAC TTATCACAAC ATTGTTGAAT TTACTGGTCA GGATTTTAAA
              541 GAAAATAGTC CAGTCTTAAC TGGAGATATG AATGTCTCAT TACCGAATGA AGTTCAACAT
              601 ATACCCAGAG ATGATGGAGT AGAATGCCCA GTGACCTTGC TTTATCCTTT ATTATCGGAT
              661 AAATCAAAAT GCGTTGAGGC TCACCAAAAT ACAATCTGCA AGCCTCTTCA TAATCAACCA
              721 GCACCTGATG TCCCATATCA CTGGATTCGT AAACAATACA CACAAAGCAN AGANGANACC
              781 GANGAANGTG ATCATATTTG TCAATCANAN ANTCTCGAAN CACACTTATA ANCGGCCGCT
              841 CGAGCATGCA TCTAGAGGGN CCTATTCTAT AGTGNCACCT AAATGNTANA NCTCGCTGAN
              901 CANCCTCNAN TGTGCCTTCN ANTTGNCAGC CATCTGTTGN TTGNCCCNCC CCCNNGNCNT
              961 NCNTGNNCCN NGNANNNGNC NNNCCNNCNG NNNNTTNNNN NTNAAATGNN NAAANNNNNN
            1021 NNNANNNTCT GANNNNNNNN NN
            //
            Primer: SV40pro_ori_F
            Region: SV40 pro_ori,NeoR
            Sequence file: RDB19609_B2Eae.seq check
            >D08836A5_B2Ea_3_SV40pro_ori_F_G04_19_ABI24.ab1
                1 NNNNNNNNNN NNNNNNGNNN CCTCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT
               61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
              121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
              181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
              241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
              301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
              361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
              421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
              481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
              541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
              601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
              661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA
              721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG
              781 CCGGCTGGGT GTGGCGGANC GCTATCANGA CATAGCGTTG GCTACCCGTG ATATTGCTGA
              841 AGANCTTGGC GGCGAATGNN CTGACCGCTT CCTCGTGCTT TACNGTATCG CCGCTCCCGA
              901 NTCGCANCGC ATCGCCTTCN ATCGCCTTCT TGANNANTNC TTCNGANCGG GNNTCTGGGG
              961 NTCNAAATGA NCGANCAAGC GNNGCCCAAN CNNGNCNTCN CNANNNTTCN ANNNNNNNNN
            1021 CNNNNNNCTA NGAAANNNNN GNNNTNNNNN NNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Hirano, M., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 2022 Apr 25. doi: 10.1038/s41587-022-01278-2. Epub ahead of print. PMID 35468954.

            2022.08.04

            GNP_filter3_RDBDEP_html_220727.pl