Please review the QC test results indicated by icon below as well as clone information before placing your order.

(n1)oxStayGold/pRSET

Fluorescent protein oxStayGold for fusion to the C-terminus of target molecule

Sequence (full)	RDB19607zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

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提供条件	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19607_B2Eap1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724) Region: 6xHis,T7 tag,Xpress tag,insert 5' Sequence file: RDB19607_B2Eaa.seq
>D08836A3_B2Ea_3_lacIpro-B_F_B05_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCGC NNCGNNTCNG TGANCGAGGA AGCGGAAGAG CGCCCAATAC    61 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGGATCTCG ATCCCGCGAA   121 ATTAATACGA CTCACTATAG GGAGACCACA ACGGTTTCCC TCTAGAAATA ATTTTGTTTA   181 ACTTTAAGAA GGAGATATAC ATATGCGGGG TTCTCATCAT CATCATCATC ATGGTATGGC   241 TAGCATGACT GGTGGACAGC AAATGGGTCG GGATCTGTAC GACGATGACG ATAAGGATCC   301 GATGGTGTCT ACAGGCGAGG AGCTGTTTAC CGGCGTGGTG CCCTTCAAGT TCCAGCTGAA   361 GGGCACCATC AACGGCAAGA GCTTCACCGT GGAAGGCGAG GGCGAGGGCA ATAGCCACGA   421 GGGCAGCCAC AAAGGCAAGT ACGTGTGCAC CAGCGGCAAA CTGCCAATGT CTTGGGCCGC   481 CCTGGGAACT AGCTTCGGCT ATGGCATGAA GTACTACACC AAGTACCCCA GCGGCCTGAA   541 GAACTGGTTC CACGAGGTGA TGCCCGAGGG CTTCACCTAC GACAGACACA TCCAGTACAA   601 GGGCGACGGC AGCATCCACG CCAAGCACCA GCACTTCATG AAGAACGGCA CCTACCACAA   661 CATCGTGGAG TTCACCGGCC AGGACTTCAA GGAGAACAGC CCCGTGCTGA CCGGCGACAT   721 GAACGTGAGC CTGCCCAACG AGGTGCAGCA CATCCCCAGA GATGACGGCG TGGANTGNCC   781 AGTGNNCCTG CTGTACCCTC TGCTGANCGA CAAGAGCAAG TGCGTGGAGG CCTACCAGAA   841 CACCATCATC AAGCCCNTGC ACAATCAGCC AGCCNCCGAT GTGCCNTACC ACTGGATCAG   901 AAAGCAGTAC ACCCAGANCN NGGNNGANAC CGANGNNAGA GANCACATCA TCCANANCGA   961 NANCNNNNNN NNCCNCCTGN NNNAANTCNN ANCTNGNTNN NNCTGNTNNC NAAGNCNNAA 1021 NNNNANCTNN NNNNNNNN //
Primer: f1ori_R3 (Pr0142) Region: T7 ter,insert 3' Sequence file: RDB19607_B2Eab.seq
>D08836A3_B2Ea_3_f1ori_R3_A05_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTTG GGANGGCGAN CGGTGCGGGC CTCTTCGCTA    61 TTACGCCAGA TCCGGATATA GTTCCTCCTT TCAGCAAAAA ACCCCTCAAG ACCCGTTTAG   121 AGGCCCCAAG GGGTTATGCT AGTTATTGCT CAGCGGTGGC AGCAGCCAAC TCAGCTTCCT   181 TTCGGGCTTT GTTAGCAGCC GGATCAAGCT TCGAATTCTT ACAGGTGGGC CTCCAGGGTC   241 TCGCTCTGGA TGATGTGGTC TCTCTCCTCG GTGTCGTCCT TGCTCTGGGT GTACTGCTTT   301 CTGATCCAGT GGTATGGCAC ATCGGGGGCT GGCTGATTGT GCAGGGGCTT GATGATGGTG   361 TTCTGGTAGG CCTCCACGCA CTTGCTCTTG TCGCTCAGCA GAGGGTACAG CAGGGTCACT   421 GGGCACTCCA CGCCGTCATC TCTGGGGATG TGCTGCACCT CGTTGGGCAG GCTCACGTTC   481 ATGTCGCCGG TCAGCACGGG GCTGTTCTCC TTGAAGTCCT GGCCGGTGAA CTCCACGATG   541 TTGTGGTAGG TGCCGTTCTT CATGAAGTGC TGGTGCTTGG CGTGGATGCT GCCGTCGCCC   601 TTGTACTGGA TGTGTCTGTC GTAGGTGAAG CCCTCGGGCA TCACCTCGTG GAACCAGTTC   661 TTCAGGCCGC TGGGGTACTT GGTGTAGTAC TTCATGCCAT AGCCGAAGCT AGTTCCCAGG   721 GCGGCCCAAG ACATTGGCAG TTTGCCGCTG GTGCACACGT ACTTGCCTTT GTGGCTGCCC   781 TCGTGGCTAT TGCCCTCGCC CTCGCCTTCC NCGGTGAANC TCTTGCCGTT GANGGTGCCC   841 TTCAGCTGGA ACTTGAANGG CACCACGCCG GTAAACAGCT CCTCGCCTGT AGANACCATC   901 GGATCCTTAT CGTCATCGTC GNACAGATCC CGANCCATTT GCTGNNCNCC AGNCATGCTA   961 NCCATANCNN GNNGANGANG NNGANGANAA CCCCNNNTAT GNNTATNNNN NTNNNNNAAG 1021 TTNANNNAAA TNNNTTNNNN NANGNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl