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Ratiometric pericam-nu/pcDNA3

Calcium ion sensor clone Ratiometric pericam-nu tagged with nuclear localization signal.

Catalog number RDB15145
Resource name Ratiometric pericam-nu/pcDNA3
Clone info. Calcium ion sensor clone Ratiometric pericam-nu having a circularly permuted YFP and a CaM. Tagged with nuclear localization signal PKKKRKVEDA. Refer Fig.1 of Nagai, T. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202, 2001 for its composition.
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
 
Other clones in our bank X. laevis (KEGG orthology K02183) | synthetic (KEGG orthology K00907) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Approval form by depositor [Word]
Remarks
Please use Schedule A (GFP-A) additionally.
Please obtain written approval of the depositor.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15145 Ratiometric pericam-nu/pcDNA3 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Calcium Ion Sensor Clones (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15145_A7H9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, insert 5'
Sequence file: RDB15145_A7H9a.seq check
>D04498D3_A7H9_1-CMV-Forward_E08_14_ABI24.ab1
    1 ACGGATTAAA TTCGGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
  121 ATACGACTCA CTATAGGGAG ACCCAAGCTT GCGGCCGCCA CCATGGTGAA GAGGCGCTGG
  181 AAGAAAAACT TCATTGCCGT CAGCGCTGCC AACCGGTTCA AGAAGATCTC CAGCTCCGGG
  241 GCACTGGGGT CTGCAGGCTA CAACAGCGAC AACGTCTATA TCATGGCCGA CAAGCAGAAG
  301 AACGGCATCA AGGCCAACTT CAAGATCCGC CACAACATCG AGGACGGCGG CGTGCAGCTC
  361 GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC
  421 CACTACCTGA GCTTCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG
  481 GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG
  541 GTCGACGGTG GCAGCGGTGG CACCGGTGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG
  601 GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC
  661 GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGCTCATCTG CACCACCGGC
  721 AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCTTCG GCTACGGCCT GAAGTGCTTC
  781 GCCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAAGC
  841 TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG
  901 GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG
  961 GAGGACGGCA ACATCCTGGG GCACAAGCTG GGAGTACAAC GGTACCGAAC CAACTGACAG
 1021 AAGAGCAGAT TGCAGAGTTC AAAGAAGCCC TTTCTCATTA TTTCGACAAG GAATGGGGAC
 1081 GGCACCATCA CCACAAAAGA CCTGCACGTT ATGAGTTCGC TTGGACAAAA ACCCAAACGG
1141 AAAGCACAGA
//
Primer: BGH_rev2
Region: bGH pA, insert 3'
Sequence file: RDB15145_A7H9b.seq check
>D04498D3_A7H9_1-BGH_rev2_F08_17_ABI24.ab1
    1 CATTACGGGG GTCAGAAGGC ACGGGGGGAG GGGCAAACAA CAGATGGCTG GCAACTAGAA
   61 GGCACAGTCG AGGCTGATCA GCGAGCTCTA GCATTTAGGT GACACTATAG AATAGGGCCC
  121 TCTAGATGCA TGCTCGAGCG GCCGCCAGTG TGATGGATAT CTGCAGAATT CTTAGGCGTC
  181 CTCCACCTTC CTCTTCTTCT TGGGCTTTGC TGTCATCATT TGTACAAACT CTTCATAGTT
  241 TACTTGGCCA TCACCATCGA TATCTGCTTC CCTTATCATT TCATCAACTT CTTCATCTGT
  301 TAACTTCTCC CCGAGGTTTG TCATGACGTG ACGTAATTCA GCAGCGCTGA TGTAGCCGTT
  361 CCCATCCTTG TCAAAAACAC GGAATGCTTC TCGGATTTCC TCTTCGCTGT CTGTGTCCTT
  421 CATTTTTCTA GCCATCATAG TAAGAAATTC AGGAAAGTAA ATCGTTCCAT TGCCATCAGC
  481 ATCGACTTCA TTGATCATAT CCTGCAATTC TGCTTCCGTT GGGTTTTGTC CAAGCGACCT
  541 CATAACGGTG CCAAGTTCCT TTGTGGTGAT GGTGCCGTCC CCATCCTTGT CGAATAATGA
  601 GAAGGCTTCT TTGAACTCTG CAATCTGCTC TTCTGTCAGT TGGTCGGTAC CGTTGTACTC
  661 CAGCTTGTGC CCCAGGATGT TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA GCTCGATGCG
  721 GTTCACCAGG GTGTCGCCCT CGAACTTCAC CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC
  781 CTTGAAGAAG ATGGTGCGCT CCTGGACGTA GCCTTCGGGG CATGGCGGAC TTGAAGAAGT
  841 CGTGCTGCTT CATGTGGTCG GGGTAGCGGG CGAAGCACTT CAGGCCGTAG CCGAAGGTGG
  901 TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAGC TTCAGGGTCA
  961 GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC GCTGAAACTT GTGGCCGGTT
 1021 TACGTCGCCC GTCCAGCTCG GACCAAGGAT GGGGCACACC CCGGGTGAAA CAGCTTCCTC
 1081 GCCCTGGCTC ACACGGTGGC CAACCGCTGG CCACGTTCGA ACTGTACAAG CTCGGTTCCA
1141 TGTCCTGAGG AGATGGAAAT TCCCTCCGGC
//
Primer: SV40pro_F
Region: SV40 pro_ori, NeoR
Sequence file: RDB15145_A7H9c.seq check
>D04498D3_A7H9_1-SV40pro_F_G08_20_ABI24.ab1
    1 ACTTGGTCCT CGGAACATTC AGAGGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
   61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG
  121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
  181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
  241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
  301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
  361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
  421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
  481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
  541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
  601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG
  661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
  721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
  781 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
  841 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA
  901 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGG TTCGAAATGA CCGACCAAGC
  961 GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
 1021 CTTTCGGGAA TCGTTTTCCC GGGACGCCGG CTGGGATGAT CCTCCCAGCG CGGGGATCTC
 1081 AATGCTGGAA GTTCTTCGCC ACCCTACCTG GTTATTTGCA GCTTATAATT GGTTACCAAC
1141 TAAAGGC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.

2020.08.06

GNP_filter3_RDBDEP_html_200316.pl