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Ratiometric pericam-nu/pcDNA3

Calcium ion sensor clone Ratiometric pericam-nu tagged with nuclear localization signal.

Catalog number RDB15145
Resource name Ratiometric pericam-nu/pcDNA3
Clone info. Calcium ion sensor clone Ratiometric pericam-nu having a circularly permuted YFP and a CaM. Tagged with nuclear localization signal PKKKRKVEDA. Refer Fig.1 of Nagai, T. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202, 2001 for its composition.
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
X. laevis CaM synthetic myosin light chain kinase peptide (M13) Aequorea victoria GFP

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202.
        4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        Schedule A (GFP-A) [Link]
        Approval form [Word] by Dr. Atsushi Miyawaki
        Remarks
        Please use Schedule A (GFP-A) additionally.
        Please obtain written approval of Dr. Atsushi Miyawaki.
        提供案内 (日本国内) [open/close]

        提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
        2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
        3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
        Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202.
        4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
        5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        Schedule A(GFP-A, 英語版のみ) [Link]
        宮脇敦史博士による提供承諾書 [Word]
        備考 提供条件は英文のまま記載してください。
        Schedule A(GFP-A, 英語版のみ)が追加で必要です。
        宮脇敦史博士の書面による提供承諾が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15145 Ratiometric pericam-nu/pcDNA3 DNA solution

        Ordering Information [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Calcium Ion Sensor Clones (English text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15145_A7H9p1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV_Forward
        Region: CMV pro, insert 5'
        Sequence file: RDB15145_A7H9a.seq check
        >D04498D3_A7H9_1-CMV-Forward_E08_14_ABI24.ab1
            1 ACGGATTAAA TTCGGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
           61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
          121 ATACGACTCA CTATAGGGAG ACCCAAGCTT GCGGCCGCCA CCATGGTGAA GAGGCGCTGG
          181 AAGAAAAACT TCATTGCCGT CAGCGCTGCC AACCGGTTCA AGAAGATCTC CAGCTCCGGG
          241 GCACTGGGGT CTGCAGGCTA CAACAGCGAC AACGTCTATA TCATGGCCGA CAAGCAGAAG
          301 AACGGCATCA AGGCCAACTT CAAGATCCGC CACAACATCG AGGACGGCGG CGTGCAGCTC
          361 GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC
          421 CACTACCTGA GCTTCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG
          481 GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG
          541 GTCGACGGTG GCAGCGGTGG CACCGGTGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG
          601 GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC
          661 GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGCTCATCTG CACCACCGGC
          721 AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCTTCG GCTACGGCCT GAAGTGCTTC
          781 GCCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAAGC
          841 TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG
          901 GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG
          961 GAGGACGGCA ACATCCTGGG GCACAAGCTG GGAGTACAAC GGTACCGAAC CAACTGACAG
         1021 AAGAGCAGAT TGCAGAGTTC AAAGAAGCCC TTTCTCATTA TTTCGACAAG GAATGGGGAC
         1081 GGCACCATCA CCACAAAAGA CCTGCACGTT ATGAGTTCGC TTGGACAAAA ACCCAAACGG
        1141 AAAGCACAGA
        //
        Primer: BGH_rev2
        Region: bGH pA, insert 3'
        Sequence file: RDB15145_A7H9b.seq check
        >D04498D3_A7H9_1-BGH_rev2_F08_17_ABI24.ab1
            1 CATTACGGGG GTCAGAAGGC ACGGGGGGAG GGGCAAACAA CAGATGGCTG GCAACTAGAA
           61 GGCACAGTCG AGGCTGATCA GCGAGCTCTA GCATTTAGGT GACACTATAG AATAGGGCCC
          121 TCTAGATGCA TGCTCGAGCG GCCGCCAGTG TGATGGATAT CTGCAGAATT CTTAGGCGTC
          181 CTCCACCTTC CTCTTCTTCT TGGGCTTTGC TGTCATCATT TGTACAAACT CTTCATAGTT
          241 TACTTGGCCA TCACCATCGA TATCTGCTTC CCTTATCATT TCATCAACTT CTTCATCTGT
          301 TAACTTCTCC CCGAGGTTTG TCATGACGTG ACGTAATTCA GCAGCGCTGA TGTAGCCGTT
          361 CCCATCCTTG TCAAAAACAC GGAATGCTTC TCGGATTTCC TCTTCGCTGT CTGTGTCCTT
          421 CATTTTTCTA GCCATCATAG TAAGAAATTC AGGAAAGTAA ATCGTTCCAT TGCCATCAGC
          481 ATCGACTTCA TTGATCATAT CCTGCAATTC TGCTTCCGTT GGGTTTTGTC CAAGCGACCT
          541 CATAACGGTG CCAAGTTCCT TTGTGGTGAT GGTGCCGTCC CCATCCTTGT CGAATAATGA
          601 GAAGGCTTCT TTGAACTCTG CAATCTGCTC TTCTGTCAGT TGGTCGGTAC CGTTGTACTC
          661 CAGCTTGTGC CCCAGGATGT TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA GCTCGATGCG
          721 GTTCACCAGG GTGTCGCCCT CGAACTTCAC CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC
          781 CTTGAAGAAG ATGGTGCGCT CCTGGACGTA GCCTTCGGGG CATGGCGGAC TTGAAGAAGT
          841 CGTGCTGCTT CATGTGGTCG GGGTAGCGGG CGAAGCACTT CAGGCCGTAG CCGAAGGTGG
          901 TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAGC TTCAGGGTCA
          961 GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC GCTGAAACTT GTGGCCGGTT
         1021 TACGTCGCCC GTCCAGCTCG GACCAAGGAT GGGGCACACC CCGGGTGAAA CAGCTTCCTC
         1081 GCCCTGGCTC ACACGGTGGC CAACCGCTGG CCACGTTCGA ACTGTACAAG CTCGGTTCCA
        1141 TGTCCTGAGG AGATGGAAAT TCCCTCCGGC
        //
        Primer: SV40pro_F
        Region: SV40 pro_ori, NeoR
        Sequence file: RDB15145_A7H9c.seq check
        >D04498D3_A7H9_1-SV40pro_F_G08_20_ABI24.ab1
            1 ACTTGGTCCT CGGAACATTC AGAGGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
           61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG
          121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
          181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
          241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
          301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
          361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
          421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
          481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
          541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
          601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG
          661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
          721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
          781 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
          841 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA
          901 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGG TTCGAAATGA CCGACCAAGC
          961 GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
         1021 CTTTCGGGAA TCGTTTTCCC GGGACGCCGG CTGGGATGAT CCTCCCAGCG CGGGGATCTC
         1081 AATGCTGGAA GTTCTTCGCC ACCCTACCTG GTTATTTGCA GCTTATAATT GGTTACCAAC
        1141 TAAAGGC
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.

        2022.02.09

        GNP_filter3_RDBDEP_html_220112.pl