RDB15145 - Ratiometric pericam-nu/pcDNA3

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Ratiometric pericam-nu/pcDNA3 (#RDB15145)

Calcium ion sensor clone Ratiometric pericam-nu tagged with nuclear localization signal.

Clone info.	Calcium ion sensor clone Ratiometric pericam-nu having a circularly permuted YFP and a CaM. Tagged with nuclear localization signal PKKKRKVEDA. Refer Fig.1 of Nagai, T. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202, 2001 for its composition.
Vector backbone	pcDNA3 (plasmid)
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name	X. laevis CaM cDNA synthetic myosin light chain kinase peptide (M13) cDNA Aequorea victoria GFP cDNA
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
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Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001).

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001).

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001).
備考	提供条件は英文のまま記載してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15145	Ratiometric pericam-nu/pcDNA3	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Ratiometric pericam-nu/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15145).

Reference section:

Nagai, T., Sawano, A., Park, E.S., Miyawaki, A., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15145_A7H9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, insert 5' Sequence file: RDB15145_A7H9a.seq
>D04498D3_A7H9_1-CMV-Forward_E08_14_ABI24.ab1 1 ACGGATTAAA TTCGGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT 61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA 121 ATACGACTCA CTATAGGGAG ACCCAAGCTT GCGGCCGCCA CCATGGTGAA GAGGCGCTGG 181 AAGAAAAACT TCATTGCCGT CAGCGCTGCC AACCGGTTCA AGAAGATCTC CAGCTCCGGG 241 GCACTGGGGT CTGCAGGCTA CAACAGCGAC AACGTCTATA TCATGGCCGA CAAGCAGAAG 301 AACGGCATCA AGGCCAACTT CAAGATCCGC CACAACATCG AGGACGGCGG CGTGCAGCTC 361 GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC 421 CACTACCTGA GCTTCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG 481 GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG 541 GTCGACGGTG GCAGCGGTGG CACCGGTGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG 601 GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC 661 GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGCTCATCTG CACCACCGGC 721 AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCTTCG GCTACGGCCT GAAGTGCTTC 781 GCCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAAGC 841 TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG 901 GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG 961 GAGGACGGCA ACATCCTGGG GCACAAGCTG GGAGTACAAC GGTACCGAAC CAACTGACAG 1021 AAGAGCAGAT TGCAGAGTTC AAAGAAGCCC TTTCTCATTA TTTCGACAAG GAATGGGGAC 1081 GGCACCATCA CCACAAAAGA CCTGCACGTT ATGAGTTCGC TTGGACAAAA ACCCAAACGG 1141 AAAGCACAGA //
Primer: BGH_rev2 (Pr0606) Region: bGH pA, insert 3' Sequence file: RDB15145_A7H9b.seq
>D04498D3_A7H9_1-BGH_rev2_F08_17_ABI24.ab1 1 CATTACGGGG GTCAGAAGGC ACGGGGGGAG GGGCAAACAA CAGATGGCTG GCAACTAGAA 61 GGCACAGTCG AGGCTGATCA GCGAGCTCTA GCATTTAGGT GACACTATAG AATAGGGCCC 121 TCTAGATGCA TGCTCGAGCG GCCGCCAGTG TGATGGATAT CTGCAGAATT CTTAGGCGTC 181 CTCCACCTTC CTCTTCTTCT TGGGCTTTGC TGTCATCATT TGTACAAACT CTTCATAGTT 241 TACTTGGCCA TCACCATCGA TATCTGCTTC CCTTATCATT TCATCAACTT CTTCATCTGT 301 TAACTTCTCC CCGAGGTTTG TCATGACGTG ACGTAATTCA GCAGCGCTGA TGTAGCCGTT 361 CCCATCCTTG TCAAAAACAC GGAATGCTTC TCGGATTTCC TCTTCGCTGT CTGTGTCCTT 421 CATTTTTCTA GCCATCATAG TAAGAAATTC AGGAAAGTAA ATCGTTCCAT TGCCATCAGC 481 ATCGACTTCA TTGATCATAT CCTGCAATTC TGCTTCCGTT GGGTTTTGTC CAAGCGACCT 541 CATAACGGTG CCAAGTTCCT TTGTGGTGAT GGTGCCGTCC CCATCCTTGT CGAATAATGA 601 GAAGGCTTCT TTGAACTCTG CAATCTGCTC TTCTGTCAGT TGGTCGGTAC CGTTGTACTC 661 CAGCTTGTGC CCCAGGATGT TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA GCTCGATGCG 721 GTTCACCAGG GTGTCGCCCT CGAACTTCAC CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC 781 CTTGAAGAAG ATGGTGCGCT CCTGGACGTA GCCTTCGGGG CATGGCGGAC TTGAAGAAGT 841 CGTGCTGCTT CATGTGGTCG GGGTAGCGGG CGAAGCACTT CAGGCCGTAG CCGAAGGTGG 901 TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAGC TTCAGGGTCA 961 GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC GCTGAAACTT GTGGCCGGTT 1021 TACGTCGCCC GTCCAGCTCG GACCAAGGAT GGGGCACACC CCGGGTGAAA CAGCTTCCTC 1081 GCCCTGGCTC ACACGGTGGC CAACCGCTGG CCACGTTCGA ACTGTACAAG CTCGGTTCCA 1141 TGTCCTGAGG AGATGGAAAT TCCCTCCGGC //
Primer: SV40pro_F (Pr0110) Region: SV40 pro_ori, NeoR Sequence file: RDB15145_A7H9c.seq
>D04498D3_A7H9_1-SV40pro_F_G08_20_ABI24.ab1 1 ACTTGGTCCT CGGAACATTC AGAGGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG 61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG 121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA 181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT 241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT 301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG 361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT 421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC 481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC 541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA 601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG 661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT 721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG 781 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC 841 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA 901 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGG TTCGAAATGA CCGACCAAGC 961 GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG 1021 CTTTCGGGAA TCGTTTTCCC GGGACGCCGG CTGGGATGAT CCTCCCAGCG CGGGGATCTC 1081 AATGCTGGAA GTTCTTCGCC ACCCTACCTG GTTATTTGCA GCTTATAATT GGTTACCAAC 1141 TAAAGGC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. [link to RRC of NBRP]