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pBS coupler 1 (#RDB15126)

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Clone info. Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |
 
Sequence (full) RDB15126hts01.seq checkAssembled from experimentally sequenced data.
Publication Shimozono, S., Methods Cell Biol. 85: 381-393 (2008). [link to RRC of NBRP]
Test sheet RDB15126_B1Dqp1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Shimozono, S,, Miyawaki, A., Methods Cell Biol. 85C: 381-393 (2008).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Shimozono, S,, Miyawaki, A., Methods Cell Biol. 85C: 381-393 (2008).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15126 pBS coupler 1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pBS coupler 1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15126).

Reference section:

Shimozono, S., Miyawaki, A., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. [link to RRC of NBRP]
user_report Rahimova, N., Development of mKO2 fusion proteins for real-time imaging and mechanistic investigation of the degradation kinetics of human IkappaBalpha in living cells. Biochim. Biophys. Acta Mol. Cell Res. 1866 (2): 190-198 (2018). PMID 30391277. [link to RRC of NBRP]