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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBS coupler 1

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Catalog number RDB15126
Resource name pBS coupler 1
Clone info. Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |
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            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Shimozono S, Miyawaki A. (2008) Engineering FRET Constructs Using CFP and YFP Methods Cell Biol. 85C: 381-393.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form             [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Shimozono S, Miyawaki A. (2008) Engineering FRET Constructs Using CFP and YFP Methods Cell Biol. 85C: 381-393.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15126 pBS coupler 1 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pBS coupler 1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15126).

            Reference section:

            Shimozono, S., Miyawaki, A., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471.

            Further references such as user reports and related articles (go to bottom)

            References and tips

            Featured content

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            Featured content Empty Backbone (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15126_A7H9p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13R ()
            Region: insert
            Sequence file: RDB15126_A7H9a.seq check
            >D04498B2_A7H9_1_M13R_F03_18_ABI24.ab1
                1 CAAGCGGCCA ATTTACCCTC ACTAAAGGGA ACAAAAGCTG GGTACCGGGC CCCCCCTCGA
               61 GGTCGACGGT ATCGATAAGC TTGATGGTGG AGGCGGTTCA GGCGGAGGTG GCTCTGGCGG
              121 TGGCGGATCG ATCGAATTCC TGCAGCCCGG GGGATCCACT AGTTCTAGAG CGGCCGCCAC
              181 CGCGGTGGAG CTCCAATTCG CCCTATAGTG AGTCGTATTA CGCGCGCTCA CTGGCCGTCG
              241 TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC
              301 ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC
              361 AGTTGCGCAG CCTGAATGGC GAATGGAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC
              421 GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC
              481 TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG
              541 TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA
              601 TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC
              661 ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA
              721 CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT
              781 AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC
              841 GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT
              901 ACATTCAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTTG
              961 AAAAAAGGAA GAGTATGAGT ATTCACATTT CCGTGTCGCC CTTATTCCCT TTTTTTGCGG
             1021 CATTTTTGCC CTTCCTTGTT TTTTGCTTCA CCCCAGAAAC GCTGGTGAAA AGTAAAAAGA
             1081 TGGCTGAAAG AATCAGTTTG GGGTGCACGA GTGGTAACAT CGACTTGGAA TTCTTCAACA
            1141 GCGGTAAAGA TTCCTTGGAG AAGGTTTTTC TTGC
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471.
            user_report Rahimova, N., Development of mKO2 fusion proteins for real-time imaging and mechanistic investigation of the degradation kinetics of human IkappaBalpha in living cells. Biochim. Biophys. Acta Mol. Cell Res. 1866 (2): 190-198 (2018). PMID 30391277.

            2023.05.22

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