Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
Clone info. | Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3]. |
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Vector backbone | Plasmid |
Depositor|Developer | Miyawaki, Atsushi | |
Sequence (full) | RDB15126hts01.seq ![]() |
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Publication | Shimozono, S., Methods Cell Biol. 85: 381-393 (2008). [link to RRC of NBRP] |
Test sheet | RDB15126_B1Dqp1-2.pdf ![]() |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Shimozono, S,, Miyawaki, A., Methods Cell Biol. 85C: 381-393 (2008). |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Shimozono, S,, Miyawaki, A., Methods Cell Biol. 85C: 381-393 (2008). |
備考 | 提供条件は英文のまま記載してください。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15126 | pBS coupler 1 | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pBS coupler 1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15126). |
Reference section:
Shimozono, S., Miyawaki, A., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. [link to RRC of NBRP] |
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user_report | Rahimova, N., Development of mKO2 fusion proteins for real-time imaging and mechanistic investigation of the degradation kinetics of human IkappaBalpha in living cells. Biochim. Biophys. Acta Mol. Cell Res. 1866 (2): 190-198 (2018). PMID 30391277. [link to RRC of NBRP] |
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