RDB15277 - mAG-hGeminin (1/60) / pT2KXIGdeltain

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

mAG-hGeminin (1/60) / pT2KXIGdeltain (#RDB15277)

hGem-based S/G2/M marker. for zebrafish cells to label nuclei with green fluorescent protein mAG.

Clone info.	hGem-based S/G2/M marker. for zebrafish cells to label nuclei with green fluorescent protein mAG.
Vector backbone	pT2KXIGdeltain (plasmid)
Size of vector backbone	8.1 kb
Selectable markers	Amp^r
Gene/insert name	human Geminin cDNA Galaxeidae stony coral GFP cDNA
Reference of insert sequence	AB505861 (DDBJ accession number)
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions:
The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. MTAに書く付加的使用条件： Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
備考	提供条件は英文のまま記載してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15277	mAG-hGeminin (1/60) / pT2KXIGdeltain	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The mAG-hGeminin (1/60) / pT2KXIGdeltain was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15277).

Reference section:

Sugiyama, M., Sakaue-Sawano, A., Iimura, T., Fukami, K., Kitaguchi, T., Kawakami, K., Okamoto, H., Higashijima, S., Miyawaki, A., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15277_A8Hop1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-N_R2 (Pr0490) Region: insert 5', Tol2R Sequence file: RDB15277_A8Hoe.seq
>15277_15277_A8Ho_1_EF1a-N_R2_E11_14_ABI24.ab1 1 TTTAACGGGC AAGATTGCAA GTTTGATTTG CATTGGAAAG GGTCGCTGGC TTTTGTGTTA 61 CACGCCCCTT ATTTGTGCTT GATTAGATGA TCCCCCTGCT CGAGGGCCCA TCTGGCCTGT 121 GTTTCAGACA CCAGGGAGTC TCTGCTCACG TTTCCTGCTA TTTGCAGCCT CTCTATCAAG 181 ACTAATACAC CTCTTCCCGC ATCGGCTGCC TGTGAGAGGC TTTTCAGCAC TGCAGGATTG 241 CTTTTCAGCC CCAAAAGAGC TAGGCTTGAC ACTAACAATT TTGAGAATCA GCTTCTACTG 301 AAGTTAAATC TGAGGTTTTA CAACTTTGAG TAGCGTGTAC TGGCATTAGA TTGTCTGTCT 361 TATAGTTTGA TAATTAAATA CAAACAGTTC TAAAGCAGGA TAAAACCTTG TATGCATTTC 421 ATTTAATGTT TTTTGAGATT AAAAGCTTAA ACAAGAATCT CTAGTTTTCT TTCTTGCTTT 481 TACTTTTACT TCCTTAATAC TCAAGTACAA TTTTAATGGA GTACTTTTTT ACTTTTACTC 541 AAGTAAGATT CTAGCCAGAT ACTTTTACTT TTAATTGAGT AAAATTTTCC CTAAGTACTT 601 GTACTTTCAC TTGAGTAAAA TTTTTGAGTA CTTTTTACAC CTCTGTCAAG AACTCCTGGA 661 CAAACCTCTG ACCTGTGTGG AACAGAGTGG ATATGGGTGT CTGAACAGAT ATTCACGTCT 721 TTTGCAGATC AGAGGGCATT TCTGGTGTTG TGCAATGACC TGGGTCCAAC AGGTAGGCGT 781 ACTCGTATCC AAGAGCATTG TTGGACAGGA GGTAGTCTCC ATTCCTATAA AGAGGAAGGA 841 ATGGCACCAT GTAGTAGCCG TCATTGTGGC CAATTGGTGC ATTGGTACGT GGGTAGATGG 901 ACCGGGGAGG CTGATGAGTT CTTAGCCAGC GCTCAAAGAT GCTAGAAATG ATGTAAGAAA 961 CAAATATGCA AGATGAAAGT TAAGATAAGA TAAAAAAAAT TTAAACAAAT AAATGCAATT 1021 ATGTTGGTGT TTCCTATTTC CACCAATAAT TGCCTCACGC AGTAAAAGCC TAACAGAATA 1081 TTTTTTTTTG ATTTCTTTTG CTTCTTGGCA AGGCCCAAAC CCCA //
Primer: pAxCALNLF1 (Pr0166) Region: insert mid (mAG-hGeminin 1_60) Sequence file: RDB15277_A8Hof.seq
>15277_15277_A8Ho_1_pAxCALNL_F1_D11_11_ABI24.ab1 1 AATAAAGATA TCTTATCATG TCTGGATCAT CATCGATTAA GATGTTAAGT GGTCATTCCG 61 ATGTTTCCTT TTGGACAAGC CTGCGGACAG CTCATTTTCT CTTCCAACAA GAGATCCAGA 121 TGCAGAAGGC TGAATCATCT TCAGAGTTCT TCTTGGGACA GAACTATTCT TTATATTCTC 181 TTTGATTTCT TCTTGTTTCT GCTTCATACT GGGATTCATC TCGAGCGGCC GCCAGTGTGA 241 TGGATATCCC TTGGCCTGGC TGGGCAGCAT GGAGTACCTG GCCACGGCGT TCTCGTACAG 301 CTTCACCTTG TTGTAGTCCT TGTCGTGCTT CAGGATCTCG ATCCTGTGGT CGATCTTGTG 361 GGCGTCGGGC AGCCTCACCT CCTTCTTGGC CTTGTAGGTG GTCTTGAAGT CGCACCTGTA 421 GTGGCCGCCG CCCTCCAGCA GCAGCCTCAT GTTCACGTCG CCCTTCAGCA CGCCGTCCTC 481 CACGTACATC TTCTCGGTGC TGGGCTCCCA CTTCAGGGTC TTCTTCTGCA TCACGGGGCC 541 GTTGGGGGGG AAGTTGGTGC CGTCGAACCT GATGTCGTAG AAGAAGCAGT CGCCCCTCAT 601 GCTGATGTTG CTGGTGGCGG TGCAGATGCC CTGGTCCTCG TAGGTCATGC TCCTCTCCCA 661 GTGGTAGCCC TCGGGGAAGG TCTGCTTGAA GTAGTCCTGG ATGTCGGCGG GGTACTTGGT 721 GAAGGCCCTG TTGCCGTACT GGAACACGGT GGTCAGGATG TCGTAGGCGA AGGGCAGGGG 781 GGCGCCCTCG GTCACGTTCA GGTCCAGGAT CTGGGTGCCC TCGTAGGGGT TGCCCTTGCC 841 CTCGCCCTCG ATCACGAAGT TGTGGCCGTT CACGGTGCCC CTCATGCACA GCTTGATCTT 901 CATCTCGGGC TTGATCACGC TCACCATGGT GGCGAAATTC CAGCACACTG GCGGCCGTTT 961 ACTAGTGGAT CCGTCGAGGG AATTCTTTTG CCCAAAATGA TGAGACAGCA CAACAAACTC 1021 AGCACGTTTG CCCAGGGAGC TGTAGGAAGA GAGACGGCAT GAACCATGTT AGCAGAGGGG 1081 CCTGGATTGG AACTCAGAGT AACTTTATCG TCATCCTCAA ACAGGTG //
Primer: pUC-CAPbs_up_F (Pr0536) Region: Tol2L, insert 3' Sequence file: RDB15277_A8Hog.seq
>15277_15277_A8Ho_1_pUC-CAP_bs_up_F_G11_20_ABI24.ab1 1 GACGACAGTG GAGCGGGGCA GTGAGCGCAA CGCATTAATG TGAGTTAGCT CACTCATTAG 61 GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA 121 TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCGCGC AATTAACCCT 181 CACTAAAGGG AACAAAAGCT GGAGCTCCAC CGCGGTGGCG GCCGCTCTAG AACTAGTGGA 241 TCTGCTGGGC TTGCTGAAGG TAGGGGGTCA AGAACCAGAG GTGTAAAGTA CTTGAGTAAT 301 TTTACTTGAT TACTGTACTT AAGTATTATT TTTGGGGATT TTTACTTTAC TTGAGTACAA 361 TTAAAAATCA ATACTTTTAC TTTTACTTAA TTACATTTTT TTAGAAAAAA AAGTACTTTT 421 TACTCCTTAC AATTTTATTT ACAGTCAAAA AGTACTTATT TTTTGGAGAT CACTTCATTC 481 TATTTTCCCT TGCTATTACC AAACCAATTG AATTGCGCTG ATGCCCAGTT TAATTTAAAT 541 GTTATTTATT CTGCCTATGA AAATCGTTTT CACATTATAT GAAATTGGTC AGACATGTTC 601 ATTGGTCCTT TGGAAGTGAC GTCATGTCAC ATCTATTACC ACAATGCACA GCACCTTGAC 661 CTGGAAATTA GGGAAATTAT AACAGTCAAT CAGTGGAAGA AAATGGAGGA AGTATGTGAT 721 TCATCAGCAG CTGCGAGCAG CACAGTCCAA AATCAGCCAC AGGATCAAGA GCACCCGTGG 781 CCGTATCTTC GCAGATCTGA TCTAGAGGAT CATAATCAGC CATACCACAT TTGTAGAGGT 841 TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC 901 AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCATAGACAT 961 CACAAATTTC ACAATAAGCA TTTTTTTCAC TGCATTCTAG TTTGTGGTTT GTCCAAACTC 1021 ATCATGTATC TTATCATGTC TGGATCATCA TCGATTAAGG AATGTAGTGG TCAATTTCCG 1081 GAATGTTTCG TTTTTGGACC AGCTGGCCGG ACAAGCTCAA TTTCCTCCTT ACCAAC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. [link to RRC of NBRP]

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Other resources in our bank

human Geminin

External Database
human Geminin

Galaxeidae stony coral GFP

Reference sequence