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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mAG-hGeminin (1/60) / pT2KXIGdeltain

hGem-based S/G2/M marker. for zebrafish cells to label nuclei with green fluorescent protein mAG.

Catalog number RDB15277
Resource name mAG-hGeminin (1/60) / pT2KXIGdeltain
Clone info. hGem-based S/G2/M marker. for zebrafish cells to label nuclei with green fluorescent protein mAG.
Vector backbone pT2KXIGdeltain (plasmid)
Size of vector backbone 8.1 kb
Selectable markers Amp^r
Gene/insert name human Geminin cDNA Galaxeidae stony coral GFP cDNA
Reference of nucleotide sequence AB505861 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
 
Other clones in our bank human GMNN (NCBI Gene 51053) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817.
3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15277 mAG-hGeminin (1/60) / pT2KXIGdeltain DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Transposon and plasmid, Kawakami laboratory (English text)
Featured content Fucci-related materials (en) (English text)
Featured content Fucci-related materials (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15277_A8Hop1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-N_R2
Region: insert 5', Tol2R
Sequence file: RDB15277_A8Hoe.seq check
>15277_15277_A8Ho_1_EF1a-N_R2_E11_14_ABI24.ab1
    1 TTTAACGGGC AAGATTGCAA GTTTGATTTG CATTGGAAAG GGTCGCTGGC TTTTGTGTTA
   61 CACGCCCCTT ATTTGTGCTT GATTAGATGA TCCCCCTGCT CGAGGGCCCA TCTGGCCTGT
  121 GTTTCAGACA CCAGGGAGTC TCTGCTCACG TTTCCTGCTA TTTGCAGCCT CTCTATCAAG
  181 ACTAATACAC CTCTTCCCGC ATCGGCTGCC TGTGAGAGGC TTTTCAGCAC TGCAGGATTG
  241 CTTTTCAGCC CCAAAAGAGC TAGGCTTGAC ACTAACAATT TTGAGAATCA GCTTCTACTG
  301 AAGTTAAATC TGAGGTTTTA CAACTTTGAG TAGCGTGTAC TGGCATTAGA TTGTCTGTCT
  361 TATAGTTTGA TAATTAAATA CAAACAGTTC TAAAGCAGGA TAAAACCTTG TATGCATTTC
  421 ATTTAATGTT TTTTGAGATT AAAAGCTTAA ACAAGAATCT CTAGTTTTCT TTCTTGCTTT
  481 TACTTTTACT TCCTTAATAC TCAAGTACAA TTTTAATGGA GTACTTTTTT ACTTTTACTC
  541 AAGTAAGATT CTAGCCAGAT ACTTTTACTT TTAATTGAGT AAAATTTTCC CTAAGTACTT
  601 GTACTTTCAC TTGAGTAAAA TTTTTGAGTA CTTTTTACAC CTCTGTCAAG AACTCCTGGA
  661 CAAACCTCTG ACCTGTGTGG AACAGAGTGG ATATGGGTGT CTGAACAGAT ATTCACGTCT
  721 TTTGCAGATC AGAGGGCATT TCTGGTGTTG TGCAATGACC TGGGTCCAAC AGGTAGGCGT
  781 ACTCGTATCC AAGAGCATTG TTGGACAGGA GGTAGTCTCC ATTCCTATAA AGAGGAAGGA
  841 ATGGCACCAT GTAGTAGCCG TCATTGTGGC CAATTGGTGC ATTGGTACGT GGGTAGATGG
  901 ACCGGGGAGG CTGATGAGTT CTTAGCCAGC GCTCAAAGAT GCTAGAAATG ATGTAAGAAA
  961 CAAATATGCA AGATGAAAGT TAAGATAAGA TAAAAAAAAT TTAAACAAAT AAATGCAATT
 1021 ATGTTGGTGT TTCCTATTTC CACCAATAAT TGCCTCACGC AGTAAAAGCC TAACAGAATA
1081 TTTTTTTTTG ATTTCTTTTG CTTCTTGGCA AGGCCCAAAC CCCA
//
Primer: pAxCALNLF1
Region: insert mid (mAG-hGeminin 1_60)
Sequence file: RDB15277_A8Hof.seq check
>15277_15277_A8Ho_1_pAxCALNL_F1_D11_11_ABI24.ab1
    1 AATAAAGATA TCTTATCATG TCTGGATCAT CATCGATTAA GATGTTAAGT GGTCATTCCG
   61 ATGTTTCCTT TTGGACAAGC CTGCGGACAG CTCATTTTCT CTTCCAACAA GAGATCCAGA
  121 TGCAGAAGGC TGAATCATCT TCAGAGTTCT TCTTGGGACA GAACTATTCT TTATATTCTC
  181 TTTGATTTCT TCTTGTTTCT GCTTCATACT GGGATTCATC TCGAGCGGCC GCCAGTGTGA
  241 TGGATATCCC TTGGCCTGGC TGGGCAGCAT GGAGTACCTG GCCACGGCGT TCTCGTACAG
  301 CTTCACCTTG TTGTAGTCCT TGTCGTGCTT CAGGATCTCG ATCCTGTGGT CGATCTTGTG
  361 GGCGTCGGGC AGCCTCACCT CCTTCTTGGC CTTGTAGGTG GTCTTGAAGT CGCACCTGTA
  421 GTGGCCGCCG CCCTCCAGCA GCAGCCTCAT GTTCACGTCG CCCTTCAGCA CGCCGTCCTC
  481 CACGTACATC TTCTCGGTGC TGGGCTCCCA CTTCAGGGTC TTCTTCTGCA TCACGGGGCC
  541 GTTGGGGGGG AAGTTGGTGC CGTCGAACCT GATGTCGTAG AAGAAGCAGT CGCCCCTCAT
  601 GCTGATGTTG CTGGTGGCGG TGCAGATGCC CTGGTCCTCG TAGGTCATGC TCCTCTCCCA
  661 GTGGTAGCCC TCGGGGAAGG TCTGCTTGAA GTAGTCCTGG ATGTCGGCGG GGTACTTGGT
  721 GAAGGCCCTG TTGCCGTACT GGAACACGGT GGTCAGGATG TCGTAGGCGA AGGGCAGGGG
  781 GGCGCCCTCG GTCACGTTCA GGTCCAGGAT CTGGGTGCCC TCGTAGGGGT TGCCCTTGCC
  841 CTCGCCCTCG ATCACGAAGT TGTGGCCGTT CACGGTGCCC CTCATGCACA GCTTGATCTT
  901 CATCTCGGGC TTGATCACGC TCACCATGGT GGCGAAATTC CAGCACACTG GCGGCCGTTT
  961 ACTAGTGGAT CCGTCGAGGG AATTCTTTTG CCCAAAATGA TGAGACAGCA CAACAAACTC
 1021 AGCACGTTTG CCCAGGGAGC TGTAGGAAGA GAGACGGCAT GAACCATGTT AGCAGAGGGG
1081 CCTGGATTGG AACTCAGAGT AACTTTATCG TCATCCTCAA ACAGGTG
//
Primer: pUC-CAPbs_up_F
Region: Tol2L, insert 3'
Sequence file: RDB15277_A8Hog.seq check
>15277_15277_A8Ho_1_pUC-CAP_bs_up_F_G11_20_ABI24.ab1
    1 GACGACAGTG GAGCGGGGCA GTGAGCGCAA CGCATTAATG TGAGTTAGCT CACTCATTAG
   61 GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA
  121 TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCGCGC AATTAACCCT
  181 CACTAAAGGG AACAAAAGCT GGAGCTCCAC CGCGGTGGCG GCCGCTCTAG AACTAGTGGA
  241 TCTGCTGGGC TTGCTGAAGG TAGGGGGTCA AGAACCAGAG GTGTAAAGTA CTTGAGTAAT
  301 TTTACTTGAT TACTGTACTT AAGTATTATT TTTGGGGATT TTTACTTTAC TTGAGTACAA
  361 TTAAAAATCA ATACTTTTAC TTTTACTTAA TTACATTTTT TTAGAAAAAA AAGTACTTTT
  421 TACTCCTTAC AATTTTATTT ACAGTCAAAA AGTACTTATT TTTTGGAGAT CACTTCATTC
  481 TATTTTCCCT TGCTATTACC AAACCAATTG AATTGCGCTG ATGCCCAGTT TAATTTAAAT
  541 GTTATTTATT CTGCCTATGA AAATCGTTTT CACATTATAT GAAATTGGTC AGACATGTTC
  601 ATTGGTCCTT TGGAAGTGAC GTCATGTCAC ATCTATTACC ACAATGCACA GCACCTTGAC
  661 CTGGAAATTA GGGAAATTAT AACAGTCAAT CAGTGGAAGA AAATGGAGGA AGTATGTGAT
  721 TCATCAGCAG CTGCGAGCAG CACAGTCCAA AATCAGCCAC AGGATCAAGA GCACCCGTGG
  781 CCGTATCTTC GCAGATCTGA TCTAGAGGAT CATAATCAGC CATACCACAT TTGTAGAGGT
  841 TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC
  901 AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCATAGACAT
  961 CACAAATTTC ACAATAAGCA TTTTTTTCAC TGCATTCTAG TTTGTGGTTT GTCCAAACTC
 1021 ATCATGTATC TTATCATGTC TGGATCATCA TCGATTAAGG AATGTAGTGG TCAATTTCCG
1081 GAATGTTTCG TTTTTGGACC AGCTGGCCGG ACAAGCTCAA TTTCCTCCTT ACCAAC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430.

2020.12.17

GNP_filter3_RDBDEP_html_201216.pl