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mito-SRAI_pcDNA3

Expression vector of fluorescent protein, mito-SRAI (signal-retaining autophagy indicator) for quantitative visualizing mitophagy.

Catalog number RDB18223
Resource name mito-SRAI_pcDNA3
Alternative name pcDNA3/mito-SRAI
Clone info. Expression vector of fluorescent protein, mito-SRAI (signal-retaining autophagy indicator), fluorescent sensor for quantitative visualizing mitophagy. SRAI is a tandem fusion of YPet and TOLLES, fused with a DNA linker that encodes a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser.
Comment The accession number in the DDBJ/EMBL/GenBank databases is [LC491576]. Ypet was created by introducing mutations into EYFP. TOLLES was cloned by introducing mutations into monomeric Azami Green (MBL). Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cells)
Gene/insert name Aequorea victoria GFP cDNA Galaxea fascicularis GFP cDNA
Reference of insert sequence LC491576 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi | Katayama, Hiroyuki |
Other clones in our bank

External Database
Aequorea victoria GFP
    Galaxea fascicularis GFP

            Reference sequence
              
             
            Sequence (partial) RDB18223pka01.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Katayama H. et al. Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. (2020). Cell 181(5), 1176–1187.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Katayama H. et al. Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. (2020). Cell 181(5), 1176–1187.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18223 mito-SRAI_pcDNA3 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The mito-SRAI_pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18223).

            Reference section:

            Katayama, H., Hama, H., Nagasawa, K., Kurokawa, H., Sugiyama, M., Ando, R., Funata, M., Yoshida, N., Homma, M., Nishimura, T., Takahashi, M., Ishida, Y., Hioki, H., Tsujihata, Y., Miyawaki, A., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660.

            Further references such as user reports and related articles (go to bottom)


            References and tips

            Featured content

            Featured content Dnaconda's recommendation EXR0112e (English text)
            Featured content Dnaconda's recommendation EXR0112j (Japanese text)
            Featured content Mitophagy (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18223_B0Ffp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward (Pr0016)
            Region: CMV pro, insert 5'
            Sequence file: RDB18223_B0Ffa.seq check
            >D07201A2_B0Ff_3_CMV_Forward_A02_02_ABI24.ab1
                1 NNNNNNNNNN NNNCNNNNTT GNNGCNNATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
               61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
              121 ACGACTCACT ATAGGGAGAC CCAAGCTTGG TACCACCATG TCCGTCCTGA CGCCGCTGCT
              181 GCTGCGGGGC TTGACAGGCT CGGCCCGGCG GCTCCCAGTG CCGCGCGCCA AGATCCATTC
              241 GTTGCCGCCG GAGGGGAAGC TTGGGATGTC CGTCCTGACG CCGCTGCTGC TGCGGGGCTT
              301 GACAGGCTCG GCCCGGCGGC TCCCAGTGCC GCGCGCCAAG ATCCATTCGT TGCCGCCGGA
              361 GGGGAAGCTC GGGGGATCCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC
              421 CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG
              481 CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGCTG CTCTGCACCA CCGGCAAGCT
              541 GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGGGCTAC GGCCTGCAGT GCTTCGCCCG
              601 CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT
              661 CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC NAGACCCGCG CCGAGGTGAA
              721 GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAN GGCATCGACT TCAANGANGA
              781 NGGCAACATC CTGGGGCACA AGCTGGANTA CAACTANNNC AGCCACAACG TCTATATCAC
              841 CNNCGANNAN CAGAAGAACN GCATCNAGGC CAACTTCANG ATNCGCCNCA ACATCNNGGA
              901 NGGNGGNNNN CANCNCGNCN ANNNCTACCA GCAGANNACC CCNNNCNNNN ANNNNCCCNN
            961 NCNNNNNNNN NANNNNNNNN NN
            //
            Primer: f1ori_R3 (Pr0142)
            Region: BGH p(A), insert 3'
            Sequence file: RDB18223_B0Ffb.seq check
            >D07201A2_B0Ef_3_f1ori_R3_C05_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTTT CNGCCTCNNA ANCCATAGAG CCCACCGCAT
               61 CCCCAGCATG CCTGCTATTG TCTTCCCAAT CCTCCCCCTT GCTGTCCTGC CCCACCCCAC
              121 CCCCCAGAAT AGAATGACAC CTACTCAGAC AATGCGATGC AATTTCCTCA TTTTATTAGG
              181 AAAGGACAGT GGGAGTGGCA CCTTCCAGGG TCAAGGAAGG CACGGGGGAG GGGCAAACAA
              241 CAGATGGCTG GCAACTAGAA GGCACAGTCG AGGCTGATCA GCGAGCTCTA GCATTTAGGT
              301 GACACTATAG AATAGGGCCC TCTAGATGCA TGCTCGAGCG GCCGCCAGTG TGATGGATAT
              361 CTGCAGAATT CCAGCACACT GGCGGCCGTT ACTAGTGGAT CCTACACATT GATCCTAGCA
              421 GAAGCACAGG CTGCAGGGTG ACGGTCCATC CCGCTCTCCT GGGCACAAGA CATGGGCAGC
              481 GTGCCATCAT CCTGCTCCTC CACCTCCGGC GGGAAGCCAT GGCTCTGGAT CTGTTTCATG
              541 AGTTGCCACA TTGGCCGTGA TCTAGCCAGG TGGATCACGA AATGGCTCAG GCTGCTGAAC
              601 CAGTTCTTGC AGGCTCTAGC CTTGGCCTGG CTGGGCAGCA TGGAGGCCCT GGCCACGGCG
              661 TTCTCGCACA GCTTCNCCTT GTTGTAGTCC TTGTCGTGCT CCAGGATCTC GATCCTGTGG
              721 TCGATCTTGT GGGCGTCNGG CANGCTCACC TCCTTCTTGG NCTTGTANGT GGTCTTGACG
              781 TCGCACCTGT AGTGGCCGCC GCCCTCCAGC ANCANCCTCA TGTTCACGTC NCCCTTCAGC
              841 ACGCCGTCCN NCACGTACAT CTTCTCGGNG NCNGGCTNCC NCTTCNNGNT CTTNTTCTGN
              901 ATCACGGGGC CNTTNGGGGG GGNANNTNGG NNNNNNNNAA NCNTNNNGNN NNANAANANN
            961 CANNNNNCCC NNNNNGCNNN NGNTNNCTNG NNNNNNGNNN NNNNNNN
            //
            Primer: EGFP-N_rev (Pr0156)
            Region: insert mid
            Sequence file: RDB18223_B0Ffc.seq check
            >D07201A2_B0Ff_3_EGFP-N_rev_F02_17_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGCN NNGGCGNNGG CGANGCCNCC TACGGCAAGC
               61 TGANCCTGAA GCTGCTCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA
              121 CCACCCTGGG CTACGGCCTG CAGTGCTTCG CCCGCTACCC CGACCACATG AAGCAGCACG
              181 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG
              241 ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC
              301 GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG
              361 AGTACAACTA CAACAGCCAC AACGTCTATA TCACCGCCGA CAAGCAGAAG AACGGCATCA
              421 AGGCCAACTT CAAGATCCGC CACAACATCG AGGACGGCGG CGTGCAGCTC GCCGACCACT
              481 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA
              541 GCTACCAGTC CGCCCTGTTC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG
              601 AGTTCCTGAC CGCCGCCGGG ATCACTGAAG GCATGAACGA GCTGTACAAG GAGCTCGGTG
              661 GAGGCGGTTC AGGCGGAGGT GGCTCTGGCG GTGGCGGATC GCTCGAGATG GTGAGCGTGA
              721 TCAAGCCCGA GATGAAGATC AAGCTGTGCA TGANGGGCAC CGTGAACGGC CACAACTTCG
              781 TGATTGAGGG CGAGGGCAAG GGCAACCCCT ACGAGGGCAC CCAGATCCTG GANCTGAACG
              841 TGACCGANGG CGCCCCCCCT GCCCTTCGCC TACGACATCC NGTCCNNNTC GTTCCAGTAC
              901 GGNNACNGGG CNNTCACCAA GTACCCCNGC CNANNTCCNN GNNNANTTCA NNCNNNCCTT
            961 NCCCNGANGG NNANNNCNGG GNNNNNNNNN N
            //
            Primer: Venus-C_F2 (Pr0454)
            Region: insert mid
            Sequence file: RDB18223_B0Ffd.seq check
            >D07201A2_B0Ff_3_Venus-C_F2_C02_08_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GNNTGNACGA GCTGTACNNA GGNAGCTCGG
               61 TGGAGGCGGT TCAGGCGGNN GTGGCTCTGG CGGTGGCGGA TCGCTCGAGA TGGTGAGCGT
              121 GATCAAGCCC GAGATGAAGA TCAAGCTGTG CATGAGGGGC ACCGTGAACG GCCACAACTT
              181 CGTGATTGAG GGCGAGGGCA AGGGCAACCC CTACGAGGGC ACCCAGATCC TGGACCTGAA
              241 CGTGACCGAG GGCGCCCCCC TGCCCTTCGC CTACGACATC CTGTCCACCT CGTTCCAGTA
              301 CGGCAACAGG GCCTTCACCA AGTACCCCGC CGACATCCAG GACTACTTCA AGCAGGCCTT
              361 CCCCGAGGGC TACCACTGGG AGAGGAGCAT GACCTACGAG GACCAGGGCA TCTGCACCGC
              421 CACCAGCAAC ATCAGCATGA GGGGCGACTG CTTCTTCTAC GACATCAGGT TCGACGGCAC
              481 CAACTTCCCC CCCAACGGCC CCGTGATGCA GAAGAAGACC CTGAAGTGGG AGCCCGACAC
              541 CGAGAAGATG TACGTGGAGG ACGGCGTGCT GAAGGGCGAC GTGAACATGA GGCTGCTGCT
              601 GGAGGGCGGC GGCCACTACA GGTGCGACGT CAAGACCACC TACAAGGCCA AGAAGGAGGT
              661 GAGCCTGCCC GACGCCCACA AGATCGACCA CAGGATCGAG ATCCTGGAGC ACGACAAGGA
              721 CTACAACAAG GTGAAGCTGT GCGAGAACGC CGTGGCCAGG GCCTCCATGC TGCCCAGCCA
              781 GGCCAAGGCT AGAGCCTGCA AGANNTGGTT CAGCAGCCTG ANCCATTTCG TGATCCACCT
              841 GGCTAGATCA CGGCCAATGN GGCAACTCAT GAAACANATC CAGANNCNTN GCTTCCCGCC
              901 NGANGNGGAN GNNCANNATG NTNGCACGCN NCCCATNNCN TNNNGCCNAN NNNANCGGGN
            961 NNGNNNCNNC NNCNNNNNNN CNNNNNNNTN NNNNNNNNGN NNNNNNNNN
            //
            Primer: SV40pro_ori_F (Pr0731)
            Region: SV40pro_ori, NeoR
            Sequence file: RDB18223_B0Ffe.seq check
            >D07201A2_B0Ff_3_SV40pro_ori_E02_14_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NCTCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT
               61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
              121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
              181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
              241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
              301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
              361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
              421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
              481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
              541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
              601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
              661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAN CTCGTCGTGA CCCATGGCGA
              721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG
              781 CCGGCTGGGT GTGGCGGANC GCTATCANGA CATANCGTTG GCTACCCGNG ATATTGCTGA
              841 AGANCTTGNC GGCGAATGGG CNGANCGCTT CCNCNNGCTT TACGGNATCG CNGCNCCNNA
              901 TTCNNANCGN ATCGCCNTCT ATCGCNTNNN NGNNNANTNC NNCNNANCNG GNNTNNNGGN
            961 NNNNNAANNN NNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Katayama, H., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660.
            user_report Hutto, R.A., Cone photoreceptors transfer damaged mitochondria to Müller glia. Cell Rep. 42 (2): 112115 (2023). PMID 36795565.

            2023.05.22

            GNP_filter3_RDBDEP_html_230512.pl