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mito-SRAI_pcDNA3 (#RDB18223)

Expression vector of fluorescent protein, mito-SRAI (signal-retaining autophagy indicator) for quantitative visualizing mitophagy.


Drawn by SnapGene® software
Sequence (full) RDB18223hts01.seq
Publication Katayama, H., Cell 181(5): 1176-1187 (2020). [link to RRC of NBRP]
Test sheet RDB18223_B0G1p1-1.pdf 
Assembled from experimentally sequenced data.
 
Alternative name pcDNA3/mito-SRAI
Clone info. Expression vector of fluorescent protein, mito-SRAI (signal-retaining autophagy indicator), fluorescent sensor for quantitative visualizing mitophagy. SRAI is a tandem fusion of YPet and TOLLES, fused with a DNA linker that encodes a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser.
Comment The accession number in the DDBJ/EMBL/GenBank databases is [LC491576]. Ypet was created by introducing mutations into EYFP. TOLLES was cloned by introducing mutations into monomeric Azami Green (MBL). Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cells)
Growth conditions LB+Amp, 37oC
Gene/insert name Aequorea victoria GFP cDNA Galaxea fascicularis GFP cDNA
Reference of insert sequence LC491576 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi | Katayama, Hiroyuki |

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Katayama H. et al., Cell 181(5): 1176-1187 (2020).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Katayama H. et al., Cell 181(5): 1176-1187 (2020).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18223 mito-SRAI_pcDNA3 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The mito-SRAI_pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18223).

Reference section:

Katayama, H., Hama, H., Nagasawa, K., Kurokawa, H., Sugiyama, M., Ando, R., Funata, M., Yoshida, N., Homma, M., Nishimura, T., Takahashi, M., Ishida, Y., Hioki, H., Tsujihata, Y., Miyawaki, A., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Katayama, H., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660. [link to RRC of NBRP]
user_report Hutto, R.A., Cone photoreceptors transfer damaged mitochondria to Müller glia. Cell Rep. 42 (2): 112115 (2023). PMID 36795565. [link to RRC of NBRP]