RDB15994 - pdsVenus

Catalog number	RDB15994
Resource name	pdsVenus
Clone info.	Destabilized Venus (dsVenus) expression vector with CMV promoter. PEST sequence was derived from mouse ornithine decarboxylase (422-461aa). For the constitution, please refer Fig. 5 of Ando, H. et al., Sci. Rep., 7(1): 12642, 2017. Please refer Nagai, T. et al., Nat. Biotechnol., 20 (1): 87-90, 2002.　PMID: 11753368 about Venus fluorescent protein.
Vector backbone	pcDNA3.1/Hygro (Plasmid)
Size of vector backbone	5.6 kb
Selectable markers	Amp^r (E. coli), Hygro^r (mammalian cells).
Gene/insert name	Mouse Odc1 cDNA
Depositor\|Developer	Miyawaki, Atsushi \| Ando, Hideaki \|

Other clones in our bank	mouse Odc1 (NCBI Gene 18263) \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai, T. et al., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol., 20 (1): 87-90, (2002). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki

Terms and conditions set forth by the DEPOSITOR

1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Nagai, T. et al., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol., 20 (1): 87-90, (2002).
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.

Ordering

Please visit Information of Request for Distribution.[link]
Order form [Credit Card Payment

] [Bank Transfer or Check Payment

]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word

]
Approval form [Word]

by Dr. Atsushi Miyawaki

Distribution information (domestic users)

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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai, T. et al., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol., 20 (1): 87-90, (2002). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15994	pdsVenus	DNA solution

original	Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737.

Featured content	Fluorescent protein resources (English text)
Featured content	Fluorescent protein resources (Japanese text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

original	Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737.
reference	Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368.

Primer: CMV-Forward Region: CMV pro, PEST, Venus 5' Sequence file: RDB15994_A8Gua.seq
>D05739A1-3_A8Gu_CMV-Forward_A01_01_ABI08.ab1 1 GCGATACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA 61 TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA 121 TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCCACCA TGAGCCATGG CTTCCCGCCG 181 GAGGTGGAGG AGCAGGATGA TGGCACGCTG CCCATGTCTT GTGCCCAGGA GAGCGGGATG 241 GACCGTCACC CTGCAGCCTG TGCTTCTGCT AGGATCAATG TGGGGAAGCT TGGTCGAGGG 301 CTAGCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC 361 GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT 421 GCCACCTACG GCAAGCTGAC CCTGAAGCTG ATCTGCACCA CCGGCAAGCT GCCCGTGCCC 481 TGGCCCACCC TCGTGACCAC CCTGGGCTAC GGCCTGCAGT GCTTCGCCCG CTACCCCGAC 541 CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC 601 ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC 661 GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC 721 CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAC CGCCGACAAG 781 CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCGGCGTG 841 CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC 901 GACAACCACT ACCTGAGCTA CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT 961 CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGAACGAGCT 1021 GTACAAGTCC CGGACTCAGA TCTAGCCCAT GGGCTTTCCG CCGGAGTGAA GGAGCAGATG 1081 ATGGGCCACG CTGCCATGTC TTGGTGCCCA AGGAGAGCGG ATGACGGTCA CCTTGCCAGC 1141 CTGTGCTCCT GCTAGGAATC AAATGGGTGT TAGAAGAGCC TTTCGCGA //
Primer: BGH_rev2 Region: BGH p(A), PEST, Venus 3' Sequence file: RDB15994_A8Gub.seq
>D05739A1-3_A8Gu_BGH_rev2_B01_02_ABI08.ab1 1 CTATTCCTAG GGGTCAGGAA GGGCACGGGG GAGGGGCAAA CAACAGATGG CTGGCAACTA 61 GAAGGCACAG TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGACTC GAGCGGCCGC 121 CACTGTGCTG GATATCTGCA GAATTCCACC ACACTGGACT AGTGGATCCC GGGCCCGCGG 181 TACCGTCGAC TGCAGAATTC GAAGCTTCTA CACATTGATC CTAGCAGAAG CACAGGCTGC 241 AGGGTGACGG TCCATCCCGC TCTCCTGGGC ACAAGACATG GGCAGCGTGC CATCATCCTG 301 CTCCTCCACC TCCGGCGGGA AGCCATGGCT AGATCTGAGT CCGGACTTGT ACAGCTCGTC 361 CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT 421 CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GTAGCTCAGG TAGTGGTTGT CGGGCAGCAG 481 CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCCGCC 541 GTCCTCGATG TTGTGGCGGA TCTTGAAGTT GGCCTTGATG CCGTTCTTCT GCTTGTCGGC 601 GGTGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC 661 GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA 721 CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG TGCGCTCCTG 781 GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA 841 GCGGGCGAAG CACTGCAGGC CGTAGCCCAG GGTGGTCACG AGGGTGGGCC AGGGCACGGG 901 CAGCTTGCCG GTGGTGCAGA TCAGCTTCAG GGTCAGCTTG CCGTAGGTGG GCATCGCCCT 961 CGCCCTCGCC GGACACGCTG AAACTTGTGG CCGTTTACGT CGCCGTTCCA GCTCGACCAG 1021 GATGGGCACC ACCCCGGGTG AACAGCCTCC TCGCCCTTGG CTCACCATGG GTGGGCTAGC 1081 CCTCGACCAA GCTTCCCCCA CCATTTGATC TAAGCAGAAG CCACAGGGCT GCAGGGTGAC 1141 GTTCATTCCC GGCCTCCTCC TGGGGCCATA GACTGGTCAG CGGTGGCATC ATCCTGTCTC 1201 ATCTCCTGTG CGGATAGAGA C //
Primer: SV40pro_F Region: SV40 pro_ori, HygR Sequence file: RDB15994_A8Guc.seq
>D05739A1-3_A8Gu_SV40pro_F_C01_03_ABI08.ab1 1 CCCCTCGGGC AATGGACCAA TTCAGAAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT 61 TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG CACGTGATGA 121 AAAAGCCTGA ACTCACCGCG ACGTCTGTCG AGAAGTTTCT GATCGAAAAG TTCGACAGCG 181 TCTCCGACCT GATGCAGCTC TCGGAGGGCG AAGAATCTCG TGCTTTCAGC TTCGATGTAG 241 GAGGGCGTGG ATATGTCCTG CGGGTAAATA GCTGCGCCGA TGGTTTCTAC AAAGATCGTT 301 ATGTTTATCG GCACTTTGCA TCGGCCGCGC TCCCGATTCC GGAAGTGCTT GACATTGGGG 361 AATTCAGCGA GAGCCTGACC TATTGCATCT CCCGCCGTGC ACAGGGTGTC ACGTTGCAAG 421 ACCTGCCTGA AACCGAACTG CCCGCTGTTC TGCAGCCGGT CGCGGAGGCC ATGGATGCGA 481 TCGCTGCGGC CGATCTTAGC CAGACGAGCG GGTTCGGCCC ATTCGGACCG CAAGGAATCG 541 GTCAATACAC TACATGGCGT GATTTCATAT GCGCGATTGC TGATCCCCAT GTGTATCACT 601 GGCAAACTGT GATGGACGAC ACCGTCAGTG CGTCCGTCGC GCAGGCTCTC GATGAGCTGA 661 TGCTTTGGGC CGAGGACTGC CCCGAAGTCC GGCACCTCGT GCACGCGGAT TTCGGCTCCA 721 ACAATGTCCT GACGGACAAT GGCCGCATAA CAGCGGTCAT TGACTGGAGC GAGGCGATGT 781 TCGGGGATTC CCAATACGAG GTCGCCAACA TCTTCTTCTG GAGGCCGTGG TTGGCTTGTA 841 TGGAGCAGCA GACGCGCTAC TTCGAGCGGA GGCATCCGGA GCTTGCAGGA TCGCCGCGGC 901 TCCGGGCGTA TATGCTCCGC ATTGGTCTTG ACCAACTCTA TCAGAGCTTG GTTGACGGCA 961 ATTTCGATGA TGCAGCTTGG GCGCAGGGTC GATGCGACGC AATCGTCCGG ATCCGGAGCC 1021 GGGACTGTCG GGCGTACACA AATCGCCCCG CAGAAGCGCG GCCGTCTGGG ACGATGGGCT 1081 GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GGACGCCCAG CACTCGGTCC GAAGGGCAAG 1141 GGTATTAGCA CGTGCTACCG AGATTTCGAT ATTCACACCG GCCGCGACT //