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pdsVenus

Destabilized Venus (dsVenus) expression vector with CMV promoter.

Catalog number RDB15994
Resource name pdsVenus
Clone info. Destabilized Venus (dsVenus) expression vector with CMV promoter. PEST sequence was derived from mouse ornithine decarboxylase (422-461aa). For the constitution, please refer Fig. 5 of Ando, H. et al., Sci. Rep., 7(1): 12642, 2017.
Please refer Nagai, T. et al., Nat. Biotechnol., 20 (1): 87-90, 2002. PMID: 11753368 about Venus fluorescent protein.
Vector backbone pcDNA3.1/Hygro (Plasmid)
Size of vector backbone 5.6 kb
Selectable markers Amp^r (E. coli), Hygro^r (mammalian cells).
Gene/insert name Mouse Odc1 cDNA
Depositor Miyawaki, Atsushi | Ando, Hideaki |
 
Other clones in our bank mouse Odc1 (NCBI Gene 18263) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Nagai, T. et al., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol., 20 (1): 87-90, (2002).
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15994 pdsVenus DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15994_A8Gup1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: CMV pro, PEST, Venus 5' | Primer: CMV-Forward | Sequence: RDB15994_A8Gua.seq
>D05739A1-3_A8Gu_CMV-Forward_A01_01_ABI08.ab1
    1 GCGATACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
   61 TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA
  121 TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCCACCA TGAGCCATGG CTTCCCGCCG
  181 GAGGTGGAGG AGCAGGATGA TGGCACGCTG CCCATGTCTT GTGCCCAGGA GAGCGGGATG
  241 GACCGTCACC CTGCAGCCTG TGCTTCTGCT AGGATCAATG TGGGGAAGCT TGGTCGAGGG
  301 CTAGCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC
  361 GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT
  421 GCCACCTACG GCAAGCTGAC CCTGAAGCTG ATCTGCACCA CCGGCAAGCT GCCCGTGCCC
  481 TGGCCCACCC TCGTGACCAC CCTGGGCTAC GGCCTGCAGT GCTTCGCCCG CTACCCCGAC
  541 CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC
  601 ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC
  661 GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC
  721 CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAC CGCCGACAAG
  781 CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCGGCGTG
  841 CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC
  901 GACAACCACT ACCTGAGCTA CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT
  961 CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGAACGAGCT
 1021 GTACAAGTCC CGGACTCAGA TCTAGCCCAT GGGCTTTCCG CCGGAGTGAA GGAGCAGATG
 1081 ATGGGCCACG CTGCCATGTC TTGGTGCCCA AGGAGAGCGG ATGACGGTCA CCTTGCCAGC
1141 CTGTGCTCCT GCTAGGAATC AAATGGGTGT TAGAAGAGCC TTTCGCGA
//
Region: BGH p(A), PEST, Venus 3' | Primer: BGH_rev2 | Sequence: RDB15994_A8Gub.seq
>D05739A1-3_A8Gu_BGH_rev2_B01_02_ABI08.ab1
    1 CTATTCCTAG GGGTCAGGAA GGGCACGGGG GAGGGGCAAA CAACAGATGG CTGGCAACTA
   61 GAAGGCACAG TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGACTC GAGCGGCCGC
  121 CACTGTGCTG GATATCTGCA GAATTCCACC ACACTGGACT AGTGGATCCC GGGCCCGCGG
  181 TACCGTCGAC TGCAGAATTC GAAGCTTCTA CACATTGATC CTAGCAGAAG CACAGGCTGC
  241 AGGGTGACGG TCCATCCCGC TCTCCTGGGC ACAAGACATG GGCAGCGTGC CATCATCCTG
  301 CTCCTCCACC TCCGGCGGGA AGCCATGGCT AGATCTGAGT CCGGACTTGT ACAGCTCGTC
  361 CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT
  421 CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GTAGCTCAGG TAGTGGTTGT CGGGCAGCAG
  481 CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCCGCC
  541 GTCCTCGATG TTGTGGCGGA TCTTGAAGTT GGCCTTGATG CCGTTCTTCT GCTTGTCGGC
  601 GGTGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC
  661 GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA
  721 CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG TGCGCTCCTG
  781 GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA
  841 GCGGGCGAAG CACTGCAGGC CGTAGCCCAG GGTGGTCACG AGGGTGGGCC AGGGCACGGG
  901 CAGCTTGCCG GTGGTGCAGA TCAGCTTCAG GGTCAGCTTG CCGTAGGTGG GCATCGCCCT
  961 CGCCCTCGCC GGACACGCTG AAACTTGTGG CCGTTTACGT CGCCGTTCCA GCTCGACCAG
 1021 GATGGGCACC ACCCCGGGTG AACAGCCTCC TCGCCCTTGG CTCACCATGG GTGGGCTAGC
 1081 CCTCGACCAA GCTTCCCCCA CCATTTGATC TAAGCAGAAG CCACAGGGCT GCAGGGTGAC
 1141 GTTCATTCCC GGCCTCCTCC TGGGGCCATA GACTGGTCAG CGGTGGCATC ATCCTGTCTC
1201 ATCTCCTGTG CGGATAGAGA C
//
Region: SV40 pro_ori, HygR | Primer: SV40pro_F | Sequence: RDB15994_A8Guc.seq
>D05739A1-3_A8Gu_SV40pro_F_C01_03_ABI08.ab1
    1 CCCCTCGGGC AATGGACCAA TTCAGAAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT
   61 TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG CACGTGATGA
  121 AAAAGCCTGA ACTCACCGCG ACGTCTGTCG AGAAGTTTCT GATCGAAAAG TTCGACAGCG
  181 TCTCCGACCT GATGCAGCTC TCGGAGGGCG AAGAATCTCG TGCTTTCAGC TTCGATGTAG
  241 GAGGGCGTGG ATATGTCCTG CGGGTAAATA GCTGCGCCGA TGGTTTCTAC AAAGATCGTT
  301 ATGTTTATCG GCACTTTGCA TCGGCCGCGC TCCCGATTCC GGAAGTGCTT GACATTGGGG
  361 AATTCAGCGA GAGCCTGACC TATTGCATCT CCCGCCGTGC ACAGGGTGTC ACGTTGCAAG
  421 ACCTGCCTGA AACCGAACTG CCCGCTGTTC TGCAGCCGGT CGCGGAGGCC ATGGATGCGA
  481 TCGCTGCGGC CGATCTTAGC CAGACGAGCG GGTTCGGCCC ATTCGGACCG CAAGGAATCG
  541 GTCAATACAC TACATGGCGT GATTTCATAT GCGCGATTGC TGATCCCCAT GTGTATCACT
  601 GGCAAACTGT GATGGACGAC ACCGTCAGTG CGTCCGTCGC GCAGGCTCTC GATGAGCTGA
  661 TGCTTTGGGC CGAGGACTGC CCCGAAGTCC GGCACCTCGT GCACGCGGAT TTCGGCTCCA
  721 ACAATGTCCT GACGGACAAT GGCCGCATAA CAGCGGTCAT TGACTGGAGC GAGGCGATGT
  781 TCGGGGATTC CCAATACGAG GTCGCCAACA TCTTCTTCTG GAGGCCGTGG TTGGCTTGTA
  841 TGGAGCAGCA GACGCGCTAC TTCGAGCGGA GGCATCCGGA GCTTGCAGGA TCGCCGCGGC
  901 TCCGGGCGTA TATGCTCCGC ATTGGTCTTG ACCAACTCTA TCAGAGCTTG GTTGACGGCA
  961 ATTTCGATGA TGCAGCTTGG GCGCAGGGTC GATGCGACGC AATCGTCCGG ATCCGGAGCC
 1021 GGGACTGTCG GGCGTACACA AATCGCCCCG CAGAAGCGCG GCCGTCTGGG ACGATGGGCT
 1081 GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GGACGCCCAG CACTCGGTCC GAAGGGCAAG
1141 GGTATTAGCA CGTGCTACCG AGATTTCGAT ATTCACACCG GCCGCGACT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737.
reference Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368.

2019.09.26

GNP_filter3_RDBDEP_html_190816.pl