Please review the QC test results indicated by icon below as well as clone information before placing your order.

pdsVenus

Destabilized Venus (dsVenus) expression vector with CMV promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai, T. et al., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol., 20 (1): 87-90, (2002). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai, T. et al., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol., 20 (1): 87-90, (2002). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15994_A8Gup1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Region: CMV pro, PEST, Venus 5' Sequence file: RDB15994_A8Gua.seq
>D05739A1-3_A8Gu_CMV-Forward_A01_01_ABI08.ab1     1 GCGATACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA    61 TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA   121 TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCCACCA TGAGCCATGG CTTCCCGCCG   181 GAGGTGGAGG AGCAGGATGA TGGCACGCTG CCCATGTCTT GTGCCCAGGA GAGCGGGATG   241 GACCGTCACC CTGCAGCCTG TGCTTCTGCT AGGATCAATG TGGGGAAGCT TGGTCGAGGG   301 CTAGCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC   361 GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT   421 GCCACCTACG GCAAGCTGAC CCTGAAGCTG ATCTGCACCA CCGGCAAGCT GCCCGTGCCC   481 TGGCCCACCC TCGTGACCAC CCTGGGCTAC GGCCTGCAGT GCTTCGCCCG CTACCCCGAC   541 CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC   601 ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC   661 GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC   721 CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAC CGCCGACAAG   781 CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCGGCGTG   841 CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC   901 GACAACCACT ACCTGAGCTA CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT   961 CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGAACGAGCT  1021 GTACAAGTCC CGGACTCAGA TCTAGCCCAT GGGCTTTCCG CCGGAGTGAA GGAGCAGATG  1081 ATGGGCCACG CTGCCATGTC TTGGTGCCCA AGGAGAGCGG ATGACGGTCA CCTTGCCAGC 1141 CTGTGCTCCT GCTAGGAATC AAATGGGTGT TAGAAGAGCC TTTCGCGA //
Primer: BGH_rev2 Region: BGH p(A), PEST, Venus 3' Sequence file: RDB15994_A8Gub.seq
>D05739A1-3_A8Gu_BGH_rev2_B01_02_ABI08.ab1     1 CTATTCCTAG GGGTCAGGAA GGGCACGGGG GAGGGGCAAA CAACAGATGG CTGGCAACTA    61 GAAGGCACAG TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGACTC GAGCGGCCGC   121 CACTGTGCTG GATATCTGCA GAATTCCACC ACACTGGACT AGTGGATCCC GGGCCCGCGG   181 TACCGTCGAC TGCAGAATTC GAAGCTTCTA CACATTGATC CTAGCAGAAG CACAGGCTGC   241 AGGGTGACGG TCCATCCCGC TCTCCTGGGC ACAAGACATG GGCAGCGTGC CATCATCCTG   301 CTCCTCCACC TCCGGCGGGA AGCCATGGCT AGATCTGAGT CCGGACTTGT ACAGCTCGTC   361 CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT   421 CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GTAGCTCAGG TAGTGGTTGT CGGGCAGCAG   481 CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCCGCC   541 GTCCTCGATG TTGTGGCGGA TCTTGAAGTT GGCCTTGATG CCGTTCTTCT GCTTGTCGGC   601 GGTGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC   661 GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA   721 CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG TGCGCTCCTG   781 GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA   841 GCGGGCGAAG CACTGCAGGC CGTAGCCCAG GGTGGTCACG AGGGTGGGCC AGGGCACGGG   901 CAGCTTGCCG GTGGTGCAGA TCAGCTTCAG GGTCAGCTTG CCGTAGGTGG GCATCGCCCT   961 CGCCCTCGCC GGACACGCTG AAACTTGTGG CCGTTTACGT CGCCGTTCCA GCTCGACCAG  1021 GATGGGCACC ACCCCGGGTG AACAGCCTCC TCGCCCTTGG CTCACCATGG GTGGGCTAGC  1081 CCTCGACCAA GCTTCCCCCA CCATTTGATC TAAGCAGAAG CCACAGGGCT GCAGGGTGAC  1141 GTTCATTCCC GGCCTCCTCC TGGGGCCATA GACTGGTCAG CGGTGGCATC ATCCTGTCTC 1201 ATCTCCTGTG CGGATAGAGA C //
Primer: SV40pro_F Region: SV40 pro_ori, HygR Sequence file: RDB15994_A8Guc.seq
>D05739A1-3_A8Gu_SV40pro_F_C01_03_ABI08.ab1     1 CCCCTCGGGC AATGGACCAA TTCAGAAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT    61 TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG CACGTGATGA   121 AAAAGCCTGA ACTCACCGCG ACGTCTGTCG AGAAGTTTCT GATCGAAAAG TTCGACAGCG   181 TCTCCGACCT GATGCAGCTC TCGGAGGGCG AAGAATCTCG TGCTTTCAGC TTCGATGTAG   241 GAGGGCGTGG ATATGTCCTG CGGGTAAATA GCTGCGCCGA TGGTTTCTAC AAAGATCGTT   301 ATGTTTATCG GCACTTTGCA TCGGCCGCGC TCCCGATTCC GGAAGTGCTT GACATTGGGG   361 AATTCAGCGA GAGCCTGACC TATTGCATCT CCCGCCGTGC ACAGGGTGTC ACGTTGCAAG   421 ACCTGCCTGA AACCGAACTG CCCGCTGTTC TGCAGCCGGT CGCGGAGGCC ATGGATGCGA   481 TCGCTGCGGC CGATCTTAGC CAGACGAGCG GGTTCGGCCC ATTCGGACCG CAAGGAATCG   541 GTCAATACAC TACATGGCGT GATTTCATAT GCGCGATTGC TGATCCCCAT GTGTATCACT   601 GGCAAACTGT GATGGACGAC ACCGTCAGTG CGTCCGTCGC GCAGGCTCTC GATGAGCTGA   661 TGCTTTGGGC CGAGGACTGC CCCGAAGTCC GGCACCTCGT GCACGCGGAT TTCGGCTCCA   721 ACAATGTCCT GACGGACAAT GGCCGCATAA CAGCGGTCAT TGACTGGAGC GAGGCGATGT   781 TCGGGGATTC CCAATACGAG GTCGCCAACA TCTTCTTCTG GAGGCCGTGG TTGGCTTGTA   841 TGGAGCAGCA GACGCGCTAC TTCGAGCGGA GGCATCCGGA GCTTGCAGGA TCGCCGCGGC   901 TCCGGGCGTA TATGCTCCGC ATTGGTCTTG ACCAACTCTA TCAGAGCTTG GTTGACGGCA   961 ATTTCGATGA TGCAGCTTGG GCGCAGGGTC GATGCGACGC AATCGTCCGG ATCCGGAGCC  1021 GGGACTGTCG GGCGTACACA AATCGCCCCG CAGAAGCGCG GCCGTCTGGG ACGATGGGCT  1081 GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GGACGCCCAG CACTCGGTCC GAAGGGCAAG 1141 GGTATTAGCA CGTGCTACCG AGATTTCGAT ATTCACACCG GCCGCGACT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl