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pdsVenus (#RDB15994)

Destabilized Venus (dsVenus) expression vector with CMV promoter.

Clone info. Destabilized Venus (dsVenus) expression vector with CMV promoter. PEST sequence was derived from mouse ornithine decarboxylase (422-461aa). For the constitution, please refer Fig. 5 of Ando, H. et al., Sci. Rep., 7(1): 12642, 2017.
Comment Please refer Nagai, T. et al., Nat. Biotechnol., 20 (1): 87-90, 2002.　PMID: 11753368 about Venus fluorescent protein.
Vector backbone pcDNA3.1/Hygro (Plasmid)
Size of vector backbone 5.6 kb
Selectable markers Amp^r (E. coli), Hygro^r (mammalian cells).
Growth conditions LB+Amp, 37oC
Gene/insert name Mouse Odc1 cDNA
Depositor|Developer Miyawaki, Atsushi | Ando, Hideaki |
 
Sequence (full) RDB15994hts01.seq checkAssembled from experimentally sequenced data.
Publication Ando, H., Sci. Rep. 7 (1): 12642 (2017). [link to RRC of NBRP]
Test sheet RDB15994_A9Ikp1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol., 20 (1): 87-90 (2002).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol., 20 (1): 87-90 (2002).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15994 pdsVenus DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pdsVenus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15994).

Reference section:

Ando, H., Hirose, M., Kurosawa, G., Impey, S., Mikoshiba, K., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737. [link to RRC of NBRP]
reference Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. [link to RRC of NBRP]