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pdsVenus

Destabilized Venus (dsVenus) expression vector with CMV promoter.

Catalog number RDB15994
Resource name pdsVenus
Clone info. Destabilized Venus (dsVenus) expression vector with CMV promoter. PEST sequence was derived from mouse ornithine decarboxylase (422-461aa). For the constitution, please refer Fig. 5 of Ando, H. et al., Sci. Rep., 7(1): 12642, 2017.
Comment Please refer Nagai, T. et al., Nat. Biotechnol., 20 (1): 87-90, 2002. PMID: 11753368 about Venus fluorescent protein.
Vector backbone pcDNA3.1/Hygro (Plasmid)
Size of vector backbone 5.6 kb
Selectable markers Amp^r (E. coli), Hygro^r (mammalian cells).
Gene/insert name Mouse Odc1 cDNA
Depositor|Developer Miyawaki, Atsushi | Ando, Hideaki |
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External Database
Mouse Odc1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Atsushi Miyawaki or an acknowledgment to Dr. Miyawaki are requested.
          Nagai, T. et al., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol., 20 (1): 87-90, (2002).
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          提供条件 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Atsushi Miyawaki or an acknowledgment to Dr. Miyawaki are requested.
          Nagai, T. et al., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol., 20 (1): 87-90, (2002).
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          備考 提供条件は英文のまま記載してください。

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          RDB15994 pdsVenus DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pdsVenus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15994).

          Reference section:

          Ando, H., Hirose, M., Kurosawa, G., Impey, S., Mikoshiba, K., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          Featured content Fluorescent protein resources (English text)
          Featured content Fluorescent protein resources (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15994_A8Gup1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,insert 5'(PEST,Venus 5')
          Sequence file: RDB15994_A8Gud.seq check
          >D05739A1-3_A8Gu_CMV-Forward_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNCNNT TGNNNNNNNN GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
             61 TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA
            121 TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCCACCA TGAGCCATGG CTTCCCGCCG
            181 GAGGTGGAGG AGCAGGATGA TGGCACGCTG CCCATGTCTT GTGCCCAGGA GAGCGGGATG
            241 GACCGTCACC CTGCAGCCTG TGCTTCTGCT AGGATCAATG TGGGGAAGCT TGGTCGAGGG
            301 CTAGCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC
            361 GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT
            421 GCCACCTACG GCAAGCTGAC CCTGAAGCTG ATCTGCACCA CCGGCAAGCT GCCCGTGCCC
            481 TGGCCCACCC TCGTGACCAC CCTGGGCTAC GGCCTGCAGT GCTTCGCCCG CTACCCCGAC
            541 CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC
            601 ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC
            661 GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC
            721 CTGGGGCACA AGCTGGAGTA CAACTACNAC AGCCACAACG TCTATATCAC CGCCGACAAG
            781 CAGAAGANNG GCATCAAGGC NNACTTCAAG ATCCGCCACA ACATCGAGGA NGGCGGCGTG
            841 CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ANGGNCCCGN GCTGCTGCCC
            901 GANAACNACT ACCTGANCTA CCAGNNCGNC CNNANCAAAG ANCCCNACNA GAANCNCGAT
          961 NNCANNNNNC NGCNNNNNTN NNNGANNNNN NNCNGNNNNA CTNNNNNNNN NN
          //
          Primer: BGH_rev2 (Pr0606)
          Region: bGH pA,insert 3'(PEST,Venus 3')
          Sequence file: RDB15994_A8Gue.seq check
          >D05739A1-3_A8Gu_BGH_rev2_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGGG GNNGGGCAAA CAACAGANGG CTGGCAACTA
             61 GAAGGCACAG TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGACTC GAGCGGCCGC
            121 CACTGTGCTG GATATCTGCA GAATTCCACC ACACTGGACT AGTGGATCCC GGGCCCGCGG
            181 TACCGTCGAC TGCAGAATTC GAAGCTTCTA CACATTGATC CTAGCAGAAG CACAGGCTGC
            241 AGGGTGACGG TCCATCCCGC TCTCCTGGGC ACAAGACATG GGCAGCGTGC CATCATCCTG
            301 CTCCTCCACC TCCGGCGGGA AGCCATGGCT AGATCTGAGT CCGGACTTGT ACAGCTCGTC
            361 CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT
            421 CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GTAGCTCAGG TAGTGGTTGT CGGGCAGCAG
            481 CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCCGCC
            541 GTCCTCGATG TTGTGGCGGA TCTTGAAGTT GGCCTTGATG CCGTTCTTCT GCTTGTCGGC
            601 GGTGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC
            661 GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA
            721 CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG TGCGCTCCTG
            781 GACNTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA
            841 GCGGGCGAAN CACTGCAGGN CGTAGCCCNN GGTGNTCNNN ANGGTGGGCC AGGGCNCGGG
            901 CAGCTTGNNN GNGGTGCANA TNANCTTCAG GGTCAGCTNG NCGNANGTGG NNNTCGCCCT
            961 CNNCNNCNNN GGANANNCNG NANCTTGTGG CNGNTNACGT CNNNNNNCNN NNNNGNNNAN
           1021 GNNGGNNNNC NNCNNNNNNN ANNNNNNTNN NNNNNNNTNN NNNANNNNNN NNNGNNNNNN
          1081 NNNN
          //
          Primer: SV40pro_F (Pr0110)
          Region: SV40 pro_ori,HygrR
          Sequence file: RDB15994_A8Guf.seq check
          >D05739A1-3_A8Gu_SV40pro_F_C01_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGTGAGGAGG NTTTTTTGGA GGCCTAGGCT
             61 TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG CACGTGATGA
            121 AAAAGCCTGA ACTCACCGCG ACGTCTGTCG AGAAGTTTCT GATCGAAAAG TTCGACAGCG
            181 TCTCCGACCT GATGCAGCTC TCGGAGGGCG AAGAATCTCG TGCTTTCAGC TTCGATGTAG
            241 GAGGGCGTGG ATATGTCCTG CGGGTAAATA GCTGCGCCGA TGGTTTCTAC AAAGATCGTT
            301 ATGTTTATCG GCACTTTGCA TCGGCCGCGC TCCCGATTCC GGAAGTGCTT GACATTGGGG
            361 AATTCAGCGA GAGCCTGACC TATTGCATCT CCCGCCGTGC ACAGGGTGTC ACGTTGCAAG
            421 ACCTGCCTGA AACCGAACTG CCCGCTGTTC TGCAGCCGGT CGCGGAGGCC ATGGATGCGA
            481 TCGCTGCGGC CGATCTTAGC CAGACGAGCG GGTTCGGCCC ATTCGGACCG CAAGGAATCG
            541 GTCAATACAC TACATGGCGT GATTTCATAT GCGCGATTGC TGATCCCCAT GTGTATCACT
            601 GGCAAACTGT GATGGACGAC ACCGTCAGTG CGTCCGTCGC GCAGGCTCTC GATGAGCTGA
            661 TGCTTTGGGC CGAGGACTGC CCCGAAGTCC GGCACCTCGT GCACGCGGAT TTCGGCTCCN
            721 ACAATGTCCT GACGGACAAT GGCCGCATAA CAGCGGTCAT TGACTGGAGC GAGGCGATGT
            781 TCGGGGATTC CNAATACGAG GTCGCCAACA TCTTCTTCTG GANGNCGTGG TTGGCTTGTA
            841 TGGAGCAGCA GANGCGCTAC TTNGAGCGGA GGCNTCCGGA NCTTGCAGGN TCGCCGCGGC
            901 NNCNGGNGTA TATGCNNCGC ATTGGNCTNG ANCNACTCTA TNANANCNNG GTTGNNGGNN
            961 ATTNCNATGA NGCANNTNNG GCGCANGGNC NATGCGANGN NNTCGTNCNN ATNNNGNNCN
          1021 NGNNNNNNNG GNNGNNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737. [link to RRC of NBRP]
          reference Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. [link to RRC of NBRP]

          2024.03.26

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