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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pdsVenus

Destabilized Venus (dsVenus) expression vector with CMV promoter.

Catalog number RDB15994
Resource name pdsVenus
Clone info. Destabilized Venus (dsVenus) expression vector with CMV promoter. PEST sequence was derived from mouse ornithine decarboxylase (422-461aa). For the constitution, please refer Fig. 5 of Ando, H. et al., Sci. Rep., 7(1): 12642, 2017.
Comment Please refer Nagai, T. et al., Nat. Biotechnol., 20 (1): 87-90, 2002. PMID: 11753368 about Venus fluorescent protein.
Vector backbone pcDNA3.1/Hygro (Plasmid)
Size of vector backbone 5.6 kb
Selectable markers Amp^r (E. coli), Hygro^r (mammalian cells).
Gene/insert name Mouse Odc1 cDNA
Depositor|Developer Miyawaki, Atsushi | Ando, Hideaki |
Other clones in our bank

External Database
Mouse Odc1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Nagai, T. et al., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol., 20 (1): 87-90, (2002).
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Nagai, T. et al., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol., 20 (1): 87-90, (2002).
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          宮脇敦史博士による提供承諾書 [Word]
          備考 提供条件は英文のまま記載してください。
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15994 pdsVenus DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Fluorescent protein resources (English text)
          Featured content Fluorescent protein resources (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15994_A8Gup1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Region: CMV pro, PEST, Venus 5'
          Sequence file: RDB15994_A8Gua.seq check
          >D05739A1-3_A8Gu_CMV-Forward_A01_01_ABI08.ab1
              1 GCGATACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
             61 TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA
            121 TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCCACCA TGAGCCATGG CTTCCCGCCG
            181 GAGGTGGAGG AGCAGGATGA TGGCACGCTG CCCATGTCTT GTGCCCAGGA GAGCGGGATG
            241 GACCGTCACC CTGCAGCCTG TGCTTCTGCT AGGATCAATG TGGGGAAGCT TGGTCGAGGG
            301 CTAGCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC
            361 GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT
            421 GCCACCTACG GCAAGCTGAC CCTGAAGCTG ATCTGCACCA CCGGCAAGCT GCCCGTGCCC
            481 TGGCCCACCC TCGTGACCAC CCTGGGCTAC GGCCTGCAGT GCTTCGCCCG CTACCCCGAC
            541 CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC
            601 ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC
            661 GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC
            721 CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAC CGCCGACAAG
            781 CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCGGCGTG
            841 CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC
            901 GACAACCACT ACCTGAGCTA CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT
            961 CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGAACGAGCT
           1021 GTACAAGTCC CGGACTCAGA TCTAGCCCAT GGGCTTTCCG CCGGAGTGAA GGAGCAGATG
           1081 ATGGGCCACG CTGCCATGTC TTGGTGCCCA AGGAGAGCGG ATGACGGTCA CCTTGCCAGC
          1141 CTGTGCTCCT GCTAGGAATC AAATGGGTGT TAGAAGAGCC TTTCGCGA
          //
          Primer: BGH_rev2
          Region: BGH p(A), PEST, Venus 3'
          Sequence file: RDB15994_A8Gub.seq check
          >D05739A1-3_A8Gu_BGH_rev2_B01_02_ABI08.ab1
              1 CTATTCCTAG GGGTCAGGAA GGGCACGGGG GAGGGGCAAA CAACAGATGG CTGGCAACTA
             61 GAAGGCACAG TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGACTC GAGCGGCCGC
            121 CACTGTGCTG GATATCTGCA GAATTCCACC ACACTGGACT AGTGGATCCC GGGCCCGCGG
            181 TACCGTCGAC TGCAGAATTC GAAGCTTCTA CACATTGATC CTAGCAGAAG CACAGGCTGC
            241 AGGGTGACGG TCCATCCCGC TCTCCTGGGC ACAAGACATG GGCAGCGTGC CATCATCCTG
            301 CTCCTCCACC TCCGGCGGGA AGCCATGGCT AGATCTGAGT CCGGACTTGT ACAGCTCGTC
            361 CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT
            421 CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GTAGCTCAGG TAGTGGTTGT CGGGCAGCAG
            481 CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCCGCC
            541 GTCCTCGATG TTGTGGCGGA TCTTGAAGTT GGCCTTGATG CCGTTCTTCT GCTTGTCGGC
            601 GGTGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC
            661 GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA
            721 CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG TGCGCTCCTG
            781 GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA
            841 GCGGGCGAAG CACTGCAGGC CGTAGCCCAG GGTGGTCACG AGGGTGGGCC AGGGCACGGG
            901 CAGCTTGCCG GTGGTGCAGA TCAGCTTCAG GGTCAGCTTG CCGTAGGTGG GCATCGCCCT
            961 CGCCCTCGCC GGACACGCTG AAACTTGTGG CCGTTTACGT CGCCGTTCCA GCTCGACCAG
           1021 GATGGGCACC ACCCCGGGTG AACAGCCTCC TCGCCCTTGG CTCACCATGG GTGGGCTAGC
           1081 CCTCGACCAA GCTTCCCCCA CCATTTGATC TAAGCAGAAG CCACAGGGCT GCAGGGTGAC
           1141 GTTCATTCCC GGCCTCCTCC TGGGGCCATA GACTGGTCAG CGGTGGCATC ATCCTGTCTC
          1201 ATCTCCTGTG CGGATAGAGA C
          //
          Primer: SV40pro_F
          Region: SV40 pro_ori, HygR
          Sequence file: RDB15994_A8Guc.seq check
          >D05739A1-3_A8Gu_SV40pro_F_C01_03_ABI08.ab1
              1 CCCCTCGGGC AATGGACCAA TTCAGAAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT
             61 TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG CACGTGATGA
            121 AAAAGCCTGA ACTCACCGCG ACGTCTGTCG AGAAGTTTCT GATCGAAAAG TTCGACAGCG
            181 TCTCCGACCT GATGCAGCTC TCGGAGGGCG AAGAATCTCG TGCTTTCAGC TTCGATGTAG
            241 GAGGGCGTGG ATATGTCCTG CGGGTAAATA GCTGCGCCGA TGGTTTCTAC AAAGATCGTT
            301 ATGTTTATCG GCACTTTGCA TCGGCCGCGC TCCCGATTCC GGAAGTGCTT GACATTGGGG
            361 AATTCAGCGA GAGCCTGACC TATTGCATCT CCCGCCGTGC ACAGGGTGTC ACGTTGCAAG
            421 ACCTGCCTGA AACCGAACTG CCCGCTGTTC TGCAGCCGGT CGCGGAGGCC ATGGATGCGA
            481 TCGCTGCGGC CGATCTTAGC CAGACGAGCG GGTTCGGCCC ATTCGGACCG CAAGGAATCG
            541 GTCAATACAC TACATGGCGT GATTTCATAT GCGCGATTGC TGATCCCCAT GTGTATCACT
            601 GGCAAACTGT GATGGACGAC ACCGTCAGTG CGTCCGTCGC GCAGGCTCTC GATGAGCTGA
            661 TGCTTTGGGC CGAGGACTGC CCCGAAGTCC GGCACCTCGT GCACGCGGAT TTCGGCTCCA
            721 ACAATGTCCT GACGGACAAT GGCCGCATAA CAGCGGTCAT TGACTGGAGC GAGGCGATGT
            781 TCGGGGATTC CCAATACGAG GTCGCCAACA TCTTCTTCTG GAGGCCGTGG TTGGCTTGTA
            841 TGGAGCAGCA GACGCGCTAC TTCGAGCGGA GGCATCCGGA GCTTGCAGGA TCGCCGCGGC
            901 TCCGGGCGTA TATGCTCCGC ATTGGTCTTG ACCAACTCTA TCAGAGCTTG GTTGACGGCA
            961 ATTTCGATGA TGCAGCTTGG GCGCAGGGTC GATGCGACGC AATCGTCCGG ATCCGGAGCC
           1021 GGGACTGTCG GGCGTACACA AATCGCCCCG CAGAAGCGCG GCCGTCTGGG ACGATGGGCT
           1081 GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GGACGCCCAG CACTCGGTCC GAAGGGCAAG
          1141 GGTATTAGCA CGTGCTACCG AGATTTCGAT ATTCACACCG GCCGCGACT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ando, H., Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Sci. Rep. 7 (1): 12642 (2017). PMID 28974737.
          reference Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl