Please review the QC test results indicated by icon below as well as clone information before placing your order.

Mermaid2

Voltage probe Mermaid2.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Tsutsui H, Jinno Y, Tomita A, Niino Y, Yamada Y, Mikoshiba K, Miyawaki A, Okamura Y. (2013) Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase. J. Physiol. 591 (18): 4427-4437. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Tsutsui H, Jinno Y, Tomita A, Niino Y, Yamada Y, Mikoshiba K, Miyawaki A, Okamura Y. (2013) Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase. J. Physiol. 591 (18): 4427-4437. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15263_A7H9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F (Pr0506) Region: CMV IE94 pro,SP6 pro, insert 5' Sequence file: RDB15263_A7H9e.seq
>D04498H3_A7H9_pCMV-IE94_F_A11_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GGGNNGNCTA TATAAGCAAT GCTCGTTTAG GGAACCGCCA    61 TTCTGCCTGG GGACGTCGGA GCTAGCTTGA TTTAGGTGAC ACTATAGAAT ACAAGCTTAT   121 GGAGGGATTC GACGGTTCAG ATTTTAGTCC TCCAGCTGAT TTAGTTGGCG TTGACGGTGC   181 AGTCATGCGG AACGTCGTTG ACGTCACGAT AAATGGTGAC GTCACTGCTC CGCCGAAAGC   241 TGCGCCAAGA AAAAGTGAAT CGGTAAAGAA AGTTCATTGG AATGATGTAG ACCAAGGACC   301 GAGTGAAAAA CCAGAGACAA GACAGGAGGA ACGAATAGAT ATACCCGAGA TTTCAGGTCT   361 ATGGTGGGGC GAGAATGAAC ATGGAGTGGA CGATGGGAGA ATGGAGATAC CTACTACTGG   421 TGTAGGGGAT CCCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT   481 GGTCGAGCTG GACGGCGACG TAAACGGCCA CAGGTTCAGC GTGAGGGGCG AGGGCGAGGG   541 CGATGCCACC CAGGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGTTGCCCGT   601 GCCCTGGCCC ACCCTCGTGA CCACCCTGAG CTGGGGCGTG CAGTGCTTCG CCCGCTACCC   661 CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGTGATG CCCGAAGGCT ACGTCCAGGA   721 GCGTACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGANG TGAAGTTCGA   781 GGGCGACACC CTGGTGAACC GCATCGAGCT GAANGGCCAG GGCTTCNAGG ANGANGGCAA   841 CATCCTGGGG CACAAGCTGG ANTACAACTA CATCAGCGAC AACGTCNATA TCACNGCCNA   901 NNAGCANAAG ANCGGCNTCN AGGCCCACTT CNAGATCCNN CNCNNNATCN NNGNNGGNGG   961 NNNNCANCTC GNNNNNCNNN NCNNNCAGAN NNNCCCCNNT CGNNNNNNNN CNNNNNCNNN 1021 NNNCNNNNNN NCNNNNNNNN NN //
Primer: pAxCALNLF1 (Pr0166) Region: insert 3' Sequence file: RDB15263_A7H9f.seq
>D04498H3_A7H9_1_pAxCALNL_F1_A05_02_ABI24.ab1     1 NNNNNNNNNN NNANNNNGTC TGGNNCTACG TAATACGACT CNCTATNGTT CTAGATTACT    61 TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA   121 TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGTAGCTC AGGTAGTGGT   181 TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA   241 GCTGCACGCC GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTGGCCTTG ATGCCGTTCT   301 TCTGCTTGTC GGCGGTGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC   361 CCAGGATGTT GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG   421 TGTCGCCCTC GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA   481 TGGTGCGCTC CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA   541 TGTGGTCGGG GTAGCGGGCG AAGCACTGCA GGCCGTAGCC CAGGGTGGTC ACGAGGGTGG   601 GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC AGATCAGCTT CAGGGTCAGC TTGCCGTAGG   661 TGGCATCGCC CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA   721 GCTCGANCAG GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATCTCGA   781 GTGANATTGT TCTTCTGCTT GAAGCCTTCA TTTGTTGGTG GGAATAAAAT ATTCTTGCTA   841 ATCGAACCAC ACGCAGCAAA CGGGCCNACA CAACNNATTG NCCCNAACCA TCNGNTNCNG   901 TTNCTTGCNC GTANTCATCT AANNCAGNGT AAAATATCGN NNNGANNANN GNNNNNNCNA 961 NNNNNNNNNC NNCANNNNNN NNNN //
Primer: VSD-M_R (Pr1312) Region: insert mid Sequence file: RDB15263_A7H9g.seq
>15263_15263_B3CmF_VSD-M_R_D01_04_ABI08.ab1     1 NNNNNNNNNN NTNNNNNNGN NANNNTGANN NCAATGATCA TGAGGANGAT GTCCAAGAAA    61 ATTAGGAAGA CTCCAAAGAC TCGCATCCCT AGATGATCAA TCACTGCTCG GACACGAAAC   121 TGGACGCGAC CTACACCGAA TTCCTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG   181 GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGC   241 CAGGACTGGG TGGTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG   301 GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT CCTCGATGTT GTGGCGGATC   361 TTGAAGTGGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTCGCTG   421 ATGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GCCCTGGCCC   481 TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG   541 TAGTTGCCGT CGTCCTTGAA GAAGATGGTA CGCTCCTGGA CGTAGCCTTC GGGCATCACG   601 GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGGCGAANCA CTGCACGCCC   661 CAGCTCANGG TGGTCACGAN GGTGGGCCAG GGCACGGGCA ACNTGNCGGN NGNGNANANG 721 NNNNTNNNGN NNNNNNTNNN NNNNGNNNNN N //
Primer: RDB15263#2 (Pr0525) Region: insert mid Sequence file: RDB15263_A7H9h.seq
>D04498H3_A7H9_RDB15263#2_C11_08_ABI24.ab1     1 NNNNNNNNTN NTNNNNNNNN NNNNNNTGAT CNTTGATCTC NGTCTTCCNG GAAAAAGTGA    61 ATCTTCACAA TCCTTTTATG ACGGGATGGC TTTGGCTCTT TCTTGTTATT TCATGCTGGA   121 TTTAGGATTA AGGATATTTG CCTACGGGCC CAAGAATTTC TTCACCAACC CCTGGGAGGT   181 TGCTGATGGT TTGATTATCG TTGTCACATT CGTCGTCACG ATATTTTACA CTGTGTTAGA   241 TGAATACGTG CAAGAAACAG GAGCCGATGG TTTGGGGCAA TTGGTTGTGT TGGCCCGTTT   301 GCTGCGTGTG GTTCGATTAG CAAGAATATT TTATTCCCAC CAACAAATGA AGGCTTCAAG   361 CAGAAGAACA ATATCACTCG AGATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT   421 GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA   481 GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG CTGATCTGCA CCACCGGCAA   541 GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC TACGGCCTGC AGTGCTTCGC   601 CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA   661 CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACNAGACCC GCGCCGAGGT   721 GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AANGGCATCG ACTTCNAGGA   781 GGANGGCAAC ATCCTGGGGC ACAAGCTGGA NTACAACTAC NACAGCCACA ACGTCTATAT   841 CACCGCCGAN AAGCAGAANA ACGGNATCAA GGCCNACTTC NAGATNCGNC ACNACATCGA   901 GGANNGCGGC GNNCAGCTCG CCNANCACTA CNAGCAGAAC ACCNNNNTCN GNNANGGCCC   961 CNNNCNGCNG NCNNANNNNN NCNANNNNAN NNANCANNNC GNCNNNANCN AANNNCCNNN 1021 NNNNNANNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl