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Mermaid2 (#RDB15263)

Voltage probe Mermaid2.

Alternative name Merm2
Clone info. Voltage probe Mermaid2.
Comment For the compornent of Mermaid2, refer Fig.S1 of Tsutsui, H., J. Physiol. 591 (18), 4427-4437, 2013. This clone has Y39Q mutation, which was confirmed by depositor, while Y39R written in the paper.
Vector backbone pCS2p+ (Plasmid)
Gene/insert name Ciona intestinalis Ci-VSP cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
 
Sequence (full) RDB15263hts01.seq checkAssembled from experimentally sequenced data.
Publication Tsutsui, H., J. Physiol. 591 (18): 4427-4437 (2013). PMID 23836686. [link to RRC of NBRP]
Test sheet RDB15263_B0Bip1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Tsutsui, H. et al., J. Physiol. 591 (18): 4427-4437 (2013).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Tsutsui, H. et al., J. Physiol. 591 (18): 4427-4437 (2013).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15263 Mermaid2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The Mermaid2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15263).

Reference section:

Tsutsui, H., Jinno, Y., Tomita, A., Niino, Y., Yamada, Y., Mikoshiba, K., Miyawaki, A., Okamura, Y., Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase. J. Physiol. 591 (18): 4427-4437 (2013). PMID 23836686. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Tsutsui, H., Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase. J. Physiol. 591 (18): 4427-4437 (2013). PMID 23836686. [link to RRC of NBRP]
review Xu, Y., Voltage imaging with genetically encoded indicators. Curr. Opin. Chem. Biol. 39: 1-10 (2017). PMID 28460291. [link to Journal]