Please review the QC test results indicated by icon below as well as clone information before placing your order.

Mermaid2

Voltage probe Mermaid2.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Tsutsui H, Jinno Y, Tomita A, Niino Y, Yamada Y, Mikoshiba K, Miyawaki A, Okamura Y. (2013) Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase. J. Physiol. 591 (18): 4427-4437. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]). Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Tsutsui H, Jinno Y, Tomita A, Niino Y, Yamada Y, Mikoshiba K, Miyawaki A, Okamura Y. (2013) Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase. J. Physiol. 591 (18): 4427-4437. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15263_A7H9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F Region: CMV IE94 pro, SP6 pro, insert 5' Sequence file: RDB15263_A7H9a.seq
>D04498H3_A7H9_pCMV-IE94_F_A11_02_ABI24.ab1     1 ATGGGCGGTA GCGTGCTAAT GGGAGGTCTA TATAAGCAAT GCTCGTTTAG GGAACCGCCA    61 TTCTGCCTGG GGACGTCGGA GCTAGCTTGA TTTAGGTGAC ACTATAGAAT ACAAGCTTAT   121 GGAGGGATTC GACGGTTCAG ATTTTAGTCC TCCAGCTGAT TTAGTTGGCG TTGACGGTGC   181 AGTCATGCGG AACGTCGTTG ACGTCACGAT AAATGGTGAC GTCACTGCTC CGCCGAAAGC   241 TGCGCCAAGA AAAAGTGAAT CGGTAAAGAA AGTTCATTGG AATGATGTAG ACCAAGGACC   301 GAGTGAAAAA CCAGAGACAA GACAGGAGGA ACGAATAGAT ATACCCGAGA TTTCAGGTCT   361 ATGGTGGGGC GAGAATGAAC ATGGAGTGGA CGATGGGAGA ATGGAGATAC CTACTACTGG   421 TGTAGGGGAT CCCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT   481 GGTCGAGCTG GACGGCGACG TAAACGGCCA CAGGTTCAGC GTGAGGGGCG AGGGCGAGGG   541 CGATGCCACC CAGGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGTTGCCCGT   601 GCCCTGGCCC ACCCTCGTGA CCACCCTGAG CTGGGGCGTG CAGTGCTTCG CCCGCTACCC   661 CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGTGATG CCCGAAGGCT ACGTCCAGGA   721 GCGTACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA   781 GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCCAG GGCTTCAAGG AGGACGGCAA   841 CATCCTGGGG CACAAGCTGG AGTACAACTA CATCAGCGAC AACGTCTATA TCACCGCCGA   901 CAAGCAGAAG AACGGCATCA AGGCCCACTT CAAGATCCGC CACAACATCG AGGACGGCGG   961 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCCAT CGGCGACGGC CCCGTGCTGC  1021 TGCCCGACAA CCCACTACCC TGACCACCCA GTCCCTGGCT GAGCAAAGAC CCCACGAGGA  1081 GCGCGATCAC ATGATCTGCC TGGAAGTCCG TGAACCGACC GGCCGGGCAT TCACCTCTTC 1141 GGTCAATTGA //
Primer: pAxCALNLF1 Region: SV40 p(A), insert 3' Sequence file: RDB15263_A7H9b.seq
>D04498H3_A7H9_1_pAxCALNL_F1_A05_02_ABI24.ab1     1 TTAATGTATC TTATCATGTC TGGATCTACG TAATACGACT CACTATAGTT CTAGATTACT    61 TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA   121 TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGTAGCTC AGGTAGTGGT   181 TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA   241 GCTGCACGCC GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTGGCCTTG ATGCCGTTCT   301 TCTGCTTGTC GGCGGTGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC   361 CCAGGATGTT GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG   421 TGTCGCCCTC GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA   481 TGGTGCGCTC CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA   541 TGTGGTCGGG GTAGCGGGCG AAGCACTGCA GGCCGTAGCC CAGGGTGGTC ACGAGGGTGG   601 GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC AGATCAGCTT CAGGGTCAGC TTGCCGTAGG   661 TGGCATCGCC CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA   721 GCTCGACCAG GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATCTCGA   781 GTGATATTGT TCTTCTGCTT GAAGCCTTCA TTTGTTGGTG GGAATAAAAT ATTCTTGCTA   841 ATCGAACCAC ACGCAGCAAA CGGGCCAACA CAACCAATTG CCCCAAACCA TCGGCTCCTG   901 TTTCTTGCAC GTATTCATCT AACACAGTGT AAAATATCGT GACGACGAAT GTGACAACGA   961 TAATCAAACC ATCAGCAACC CTCCCAGGGT TGGGTGAAGA AATTCTTGGC CCGTAGGCAA  1021 ATATCCCTTT AATTCCTTAA ATCAGCATGA AATACAGAAA GAGCAAGGTC ATCCGGTCAT 1081 AAATGATGTG AGATTCACCA TTCTGCGACT GGAGATCGAA TGAAT //
Primer: RDB15263#1 Region: insert mid Sequence file: RDB15263_A7H9c.seq
>D04498H3_A7H9_RDB15263#1_B11_05_ABI24.ab1     1 GTTTTTTCCT GGGAGATGGG GATCATGATC ATGAGGATGA TGTCCAAGAA AATTAGGAAG    61 ACTCCAAAGA CTCGCATCCC TAGATGATCA ATCACTGCTC GGACACGAAA CTGGACGCGA   121 CCTACACCGA ATTCCTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG   181 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG CCAGGACTGG   241 GTGGTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC   301 TGGTAGTGGT CGGCGAGCTG CACGCCGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTGG   361 GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTCGCT GATGTAGTTG   421 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGCCCTGGCC CTTCAGCTCG   481 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG   541 TCGTCCTTGA AGAAGATGGT ACGCTCCTGG ACGTAGCCTT CGGGCATCAC GGGACTTGAA   601 GAAGTCGTGC TGCTTCATGT GGTCGGGGGT AGCGGGCGAA GCACTGCACG CCCCAGCTCA   661 GGGTGGTCAC GGAGGGTGGG GCCAGGGGCA CGGGCAACTT GCCGGTGGTG CAGATGAACT   721 TCAGGGTCAG CTTGCCCTGG GGTGGGCATC GCCCTCGGCC CTCGCCCCTC ACGCTGAACC   781 TGTGGGCCGT TTTACGTCGC CCGTCCAGCT CGACCAGGGA TGGGCACCAC CCCCGGTGAA   841 CAGCTCCTCG CCCCTTGCTC ACCATGGGGG ATCCCCCTAC ACCAGGAGTA GGTATCTCCA   901 TTCTCCCATC GTCCACTCCC ATGTTCATTT CTCGCCCAGC ATTAGACCTG GAATCTTGGG 961 G //
Primer: RDB15263#2 Region: insert mid Sequence file: RDB15263_A7H9d.seq
>D04498H3_A7H9_RDB15263#2_C11_08_ABI24.ab1     1 TACTCGACTT CTTGGAATCA TCCTCATGAT CATTGATCTC AGTCTTCCAG GAAAAAGTGA    61 ATCTTCACAA TCCTTTTATG ACGGGATGGC TTTGGCTCTT TCTTGTTATT TCATGCTGGA   121 TTTAGGATTA AGGATATTTG CCTACGGGCC CAAGAATTTC TTCACCAACC CCTGGGAGGT   181 TGCTGATGGT TTGATTATCG TTGTCACATT CGTCGTCACG ATATTTTACA CTGTGTTAGA   241 TGAATACGTG CAAGAAACAG GAGCCGATGG TTTGGGGCAA TTGGTTGTGT TGGCCCGTTT   301 GCTGCGTGTG GTTCGATTAG CAAGAATATT TTATTCCCAC CAACAAATGA AGGCTTCAAG   361 CAGAAGAACA ATATCACTCG AGATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT   421 GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA   481 GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG CTGATCTGCA CCACCGGCAA   541 GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC TACGGCCTGC AGTGCTTCGC   601 CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA   661 CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT   721 GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA   781 GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT   841 CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC AAGATCCGCC ACAACATCGA   901 GGACGGCGGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC   961 CGTGCTGCTG CCCGACAACC ACTACCTGAG CTACCAGTCC GCCCTGAGCA AAGACCCCAA  1021 CGAGAAAGCG CGATCACATG GGTCCCTGCT GGAGTTTCGT GACCGCCCGC CGGGATCACT  1081 CTCGGCATGG GACGAGCTGT ACCAGGTTAT CCTAGGACTA TAGCTGGAGT CGTATTACGT 1141 AGAATCCAGG AACCAT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.13

GNP_filter3_RDBDEP_html_220713.pl