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Mermaid2

Voltage probe Mermaid2.

Catalog number RDB15263
Resource name Mermaid2
Alternative name Merm2
Clone info. Voltage probe Mermaid2.
For the compornent of Mermaid2, refer Fig.S1 of Tsutsui, H., J. Physiol. 591 (18), 4427-4437, 2013. This clone has Y39Q mutation, which was confirmed by depositor, while Y39R written in the paper.
Vector backbone pCS2p+ (Plasmid)
Gene/insert name Ciona intestinalis Ci-VSP cDNA Aequorea victoria GFP cDNA
Depositor Miyawaki, Atsushi |
 
Other clones in our bank Ciona intestinalis vsp (KEGG orthology K18079) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Tsutsui H, Jinno Y, Tomita A, Niino Y, Yamada Y, Mikoshiba K, Miyawaki A, Okamura Y. (2013) Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase. J. Physiol. 591 (18): 4427-4437.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Approval form by depositor [Word]
Remarks
Please use Schedule A (GFP-A) additionally.
Please obtain written approval of the depositor.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15263 Mermaid2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Dr. Miyawaki's resources (English text)
Featured content Sensor and Visualization (English text)

References

original Tsutsui, H., Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase. J. Physiol. 591 (18): 4427-4437 (2013). PMID 23836686.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15263_A7H9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F RDB15263_A7H9a.seq
>D04498H3_A7H9_pCMV-IE94_F_A11_02_ABI24.ab1
    1 ATGGGCGGTA GCGTGCTAAT GGGAGGTCTA TATAAGCAAT GCTCGTTTAG GGAACCGCCA
   61 TTCTGCCTGG GGACGTCGGA GCTAGCTTGA TTTAGGTGAC ACTATAGAAT ACAAGCTTAT
  121 GGAGGGATTC GACGGTTCAG ATTTTAGTCC TCCAGCTGAT TTAGTTGGCG TTGACGGTGC
  181 AGTCATGCGG AACGTCGTTG ACGTCACGAT AAATGGTGAC GTCACTGCTC CGCCGAAAGC
  241 TGCGCCAAGA AAAAGTGAAT CGGTAAAGAA AGTTCATTGG AATGATGTAG ACCAAGGACC
  301 GAGTGAAAAA CCAGAGACAA GACAGGAGGA ACGAATAGAT ATACCCGAGA TTTCAGGTCT
  361 ATGGTGGGGC GAGAATGAAC ATGGAGTGGA CGATGGGAGA ATGGAGATAC CTACTACTGG
  421 TGTAGGGGAT CCCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT
  481 GGTCGAGCTG GACGGCGACG TAAACGGCCA CAGGTTCAGC GTGAGGGGCG AGGGCGAGGG
  541 CGATGCCACC CAGGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGTTGCCCGT
  601 GCCCTGGCCC ACCCTCGTGA CCACCCTGAG CTGGGGCGTG CAGTGCTTCG CCCGCTACCC
  661 CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGTGATG CCCGAAGGCT ACGTCCAGGA
  721 GCGTACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA
  781 GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCCAG GGCTTCAAGG AGGACGGCAA
  841 CATCCTGGGG CACAAGCTGG AGTACAACTA CATCAGCGAC AACGTCTATA TCACCGCCGA
  901 CAAGCAGAAG AACGGCATCA AGGCCCACTT CAAGATCCGC CACAACATCG AGGACGGCGG
  961 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCCAT CGGCGACGGC CCCGTGCTGC
 1021 TGCCCGACAA CCCACTACCC TGACCACCCA GTCCCTGGCT GAGCAAAGAC CCCACGAGGA
 1081 GCGCGATCAC ATGATCTGCC TGGAAGTCCG TGAACCGACC GGCCGGGCAT TCACCTCTTC
1141 GGTCAATTGA
//
Primer: pAxCALNL_F1 RDB15263_A7H9b.seq
>D04498H3_A7H9_1_pAxCALNL_F1_A05_02_ABI24.ab1
    1 TTAATGTATC TTATCATGTC TGGATCTACG TAATACGACT CACTATAGTT CTAGATTACT
   61 TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA
  121 TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGTAGCTC AGGTAGTGGT
  181 TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA
  241 GCTGCACGCC GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTGGCCTTG ATGCCGTTCT
  301 TCTGCTTGTC GGCGGTGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC
  361 CCAGGATGTT GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG
  421 TGTCGCCCTC GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA
  481 TGGTGCGCTC CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA
  541 TGTGGTCGGG GTAGCGGGCG AAGCACTGCA GGCCGTAGCC CAGGGTGGTC ACGAGGGTGG
  601 GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC AGATCAGCTT CAGGGTCAGC TTGCCGTAGG
  661 TGGCATCGCC CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA
  721 GCTCGACCAG GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATCTCGA
  781 GTGATATTGT TCTTCTGCTT GAAGCCTTCA TTTGTTGGTG GGAATAAAAT ATTCTTGCTA
  841 ATCGAACCAC ACGCAGCAAA CGGGCCAACA CAACCAATTG CCCCAAACCA TCGGCTCCTG
  901 TTTCTTGCAC GTATTCATCT AACACAGTGT AAAATATCGT GACGACGAAT GTGACAACGA
  961 TAATCAAACC ATCAGCAACC CTCCCAGGGT TGGGTGAAGA AATTCTTGGC CCGTAGGCAA
 1021 ATATCCCTTT AATTCCTTAA ATCAGCATGA AATACAGAAA GAGCAAGGTC ATCCGGTCAT
1081 AAATGATGTG AGATTCACCA TTCTGCGACT GGAGATCGAA TGAAT
//
Primer: RDB15263#1 RDB15263_A7H9c.seq
>D04498H3_A7H9_RDB15263#1_B11_05_ABI24.ab1
    1 GTTTTTTCCT GGGAGATGGG GATCATGATC ATGAGGATGA TGTCCAAGAA AATTAGGAAG
   61 ACTCCAAAGA CTCGCATCCC TAGATGATCA ATCACTGCTC GGACACGAAA CTGGACGCGA
  121 CCTACACCGA ATTCCTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG
  181 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG CCAGGACTGG
  241 GTGGTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC
  301 TGGTAGTGGT CGGCGAGCTG CACGCCGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTGG
  361 GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTCGCT GATGTAGTTG
  421 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGCCCTGGCC CTTCAGCTCG
  481 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG
  541 TCGTCCTTGA AGAAGATGGT ACGCTCCTGG ACGTAGCCTT CGGGCATCAC GGGACTTGAA
  601 GAAGTCGTGC TGCTTCATGT GGTCGGGGGT AGCGGGCGAA GCACTGCACG CCCCAGCTCA
  661 GGGTGGTCAC GGAGGGTGGG GCCAGGGGCA CGGGCAACTT GCCGGTGGTG CAGATGAACT
  721 TCAGGGTCAG CTTGCCCTGG GGTGGGCATC GCCCTCGGCC CTCGCCCCTC ACGCTGAACC
  781 TGTGGGCCGT TTTACGTCGC CCGTCCAGCT CGACCAGGGA TGGGCACCAC CCCCGGTGAA
  841 CAGCTCCTCG CCCCTTGCTC ACCATGGGGG ATCCCCCTAC ACCAGGAGTA GGTATCTCCA
  901 TTCTCCCATC GTCCACTCCC ATGTTCATTT CTCGCCCAGC ATTAGACCTG GAATCTTGGG
961 G
//
Primer: RDB15263#2 RDB15263_A7H9d.seq
>D04498H3_A7H9_RDB15263#2_C11_08_ABI24.ab1
    1 TACTCGACTT CTTGGAATCA TCCTCATGAT CATTGATCTC AGTCTTCCAG GAAAAAGTGA
   61 ATCTTCACAA TCCTTTTATG ACGGGATGGC TTTGGCTCTT TCTTGTTATT TCATGCTGGA
  121 TTTAGGATTA AGGATATTTG CCTACGGGCC CAAGAATTTC TTCACCAACC CCTGGGAGGT
  181 TGCTGATGGT TTGATTATCG TTGTCACATT CGTCGTCACG ATATTTTACA CTGTGTTAGA
  241 TGAATACGTG CAAGAAACAG GAGCCGATGG TTTGGGGCAA TTGGTTGTGT TGGCCCGTTT
  301 GCTGCGTGTG GTTCGATTAG CAAGAATATT TTATTCCCAC CAACAAATGA AGGCTTCAAG
  361 CAGAAGAACA ATATCACTCG AGATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT
  421 GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA
  481 GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG CTGATCTGCA CCACCGGCAA
  541 GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC TACGGCCTGC AGTGCTTCGC
  601 CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA
  661 CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT
  721 GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA
  781 GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT
  841 CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC AAGATCCGCC ACAACATCGA
  901 GGACGGCGGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC
  961 CGTGCTGCTG CCCGACAACC ACTACCTGAG CTACCAGTCC GCCCTGAGCA AAGACCCCAA
 1021 CGAGAAAGCG CGATCACATG GGTCCCTGCT GGAGTTTCGT GACCGCCCGC CGGGATCACT
 1081 CTCGGCATGG GACGAGCTGT ACCAGGTTAT CCTAGGACTA TAGCTGGAGT CGTATTACGT
1141 AGAATCCAGG AACCAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tsutsui, H., Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase. J. Physiol. 591 (18): 4427-4437 (2013). PMID 23836686.
reference Xu, Y., Voltage imaging with genetically encoded indicators. Curr. Opin. Chem. Biol. 39: 1-10 (2017). PMID 28460291.

2019.02.26

GNP_filter3_RDBDEP_html_190131.pl