RDB15780 - ptol2/cmlc2-mermaid

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icon below as well as clone information before placing your order.

ptol2/cmlc2-mermaid (#RDB15780)

Voltage indicator Mermaid.

Clone info.	Voltage indicator Mermaid.
Vector backbone	pT2KXIG delta in (Plasmid)
Gene/insert name	Ciona intestinalis Ci-VSP cDNA Sarcophyton sp. GFP (Umikinoko-green) cDNA Fungia concinna OFP (Kusabira Orange) cDNA
Depositor\|Developer	Miyawaki, Atsushi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Tsutsui H, Higashijima SI, Miyawaki A, Okamura Y. (2010) Visualizing voltage dynamics in zebrafish heart. J. Physiol., 588 (Pt 12): 2017-2021.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions:
The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Tsutsui H, Higashijima SI, Miyawaki A, Okamura Y. (2010) Visualizing voltage dynamics in zebrafish heart. J. Physiol., 588 (Pt 12): 2017-2021. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Tsutsui H, Higashijima SI, Miyawaki A, Okamura Y. (2010) Visualizing voltage dynamics in zebrafish heart. J. Physiol., 588 (Pt 12): 2017-2021. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. MTAに書く付加的使用条件： Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15780	ptol2/cmlc2-mermaid	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The ptol2/cmlc2-mermaid was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15780).

Reference section:

Tsutsui, H., Higashijima, S., Miyawaki, A., Okamura, Y., Visualizing voltage dynamics in zebrafish heart. J. Physiol. 588 (Pt 12): 2017-2021 (2010). PMID 20421282. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15780_A8D8p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: cmlc2pro_F (Pr0643) Region: insert mid(cmlc2 pro,Mermaid 5') Sequence file: RDB15780_A8D8f.seq
>D04498J8_A8D8_3_cmlc2pro_F_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNNNAGAGT CCTCNTACNT AAAGACTCCN TTAGTAAGCC AGTGACCCAG 61 GAGCCCAGAC CAACAGCAAA GCAGACAGTG GATCCACTAG TTCTAGAGCG GCCGCATGGA 121 GGGATTCGAC GGTTCAGATT TTAGTCCTCC AGCTGATTTA GTTGGCGTTG ACGGTGCAGT 181 CATGCGGAAC GTCGTTGACG TCACGATAAA TGGTGACGTC ACTGCTCCGC CGAAAGCTGC 241 GCCAAGAAAA AGTGAATCGG TAAAGAAAGT TCATTGGAAT GATGTAGACC AAGGACCGAG 301 TGAAAAACCA GAGACAAGAC AGGAGGAACG AATAGATATA CCCGAGATTT CAGGTCTATG 361 GTGGGGCGAG AATGAACATG GAGTGGACGA TGGGAGAATG GAGATACCTA CTACTGGTGT 421 AGGTCGCGTC CAGTTTCGTG TCCGAGCAGT GATTGATCAT CTAGGGATGC GAGTCTTTGG 481 AGTCTTCCTA ATTTTCTTGG ACATCATCCT CATGATCATT GATCTCAGTC TTCCAGGAAA 541 AAGTGAATCT TCACAATCCT TTTATGACGG GATGGCTTTG GCTCTTTCTT GTTATTTCAT 601 GCTGGATTTA GGATTAAGGA TATTTGCCTA CGGGCCCAAG AATTTCTTCA CCAACCCCTG 661 GGAGGTTGCT GATGGTTTGA TTATCGTTGT CACATTCGTC GTCACGATAT TTTACACTGT 721 GTTAGATGAA TACGTGCAAG AAACAGGAGC CGATGGTTTG GGGCAATTGG TTGTGTTGGC 781 CCGTTTGCTG CGTGTGGTTC GATTAGCAAG AATATTTTAT TCCCACCAAC AAATGAANGN 841 TTCAAGCAGA AGAACAATAT CANGGGNNCC CATGANTGTG ATTAAAGANG AAATGAAGAT 901 CAAGCTGCAT ATGGAAGGAA ATGTNAANNN NCNTGNNTTT GTGATTGNNN NNNANGGNAA 961 NNNAAAGNNT TANNATGGGN NNCAGANTTT NAANNNGANA NTGNANNNNN NNNNN //
Primer: mKOk-C_F (Pr0645) Region: insert 3'(Marmeid 3' SV40 pA,delta Tol2 ITR ) Sequence file: RDB15780_A8D8g.seq
>D04498J8_A8D8_3_mKOk-C_F_H03_24_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NACNTCGGCN NTCGCCTCGT CNGGAAAACC GAAGGCAACA 61 TTACTGAGCA GGTAGAAGAT GCAGTAGCTC ATTCCTAATC TAGATCATAA TCAGCCATAC 121 CACATTTGTA GAGGTTTTAC TTGCTTTAAA AAACCTCCCA CACCTCCCCC TGAACCTGAA 181 ACATAAAATG AATGCAATTG TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA 241 ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG 301 TGGTTTGTCC AAACTCATCA ATGTATGCGG CCGCACTAGT CTCGAGTCTA GAGATCTGCG 361 AAGATACGGC CACGGGTGCT CTTGATCCTG TGGCTGATTT TGGACTGTGC TGCTCGCAGC 421 TGCTGATGAA TCACATACTT CCTCCATTTT CTTCCACTGA TTGACTGTTA TAATTTCCCT 481 AATTTCCAGG TCAAGGTGCT GTGCATTGTG GTAATAGATG TGACATGACG TCACTTCCAA 541 AGGACCAATG AACATGTCTG ACCAATTTCA TATAATGTGA AAACGATTTT CATAGGCAGA 601 ATAAATAACA TTTAAATTAA ACTGGGCATC AGCGCAATTC AATTGGTTTG GTAATAGCAA 661 GGGAAAATAG AATGAAGTGA TCTCCAAAAA ATAAGTACTT TTTGACTGTA AATAAAATTG 721 TAAGGAGTAA AAAGTACTTT TTTTTCTAAA AAAATGTAAT TAAGTAAAAG TAAAAGTATT 781 GATTTTTAAT TGTACTCNAG TAAAGTAAAA ATCNNNAAAA ATAATACTTA AGTACAGTAA 841 TCAAGTAAAA TTACTCNAGT ACTTTACACC TCTGGNTCTN GANCCCCTAC CTTCAGCNAG 901 CCCAGCANAT NCNCTANGGC CGCCNCCGCN GNGGANCTCC AGCTTTTGNN CCCTTTANTG 961 AGGGNTAATT GNNCNCTNGG CGNNNNNNTG NNCNTANCTN NTTNNNGNGT GAAATTGNNA 1021 TNNNNNNNNN ATNNNNNNNN NN //
Primer: mKO2-N_R (Pr0494) Region: insert mid(Mermaid mid) Sequence file: RDB15780_A8D8h.seq
>D04498J8_A8D8_3_mK02-N_R_B03_06_ABI24.ab1 1 NNNNNNNNNN NNNNNNTGCN NNTNGNNGGN NCCGTCNNTG TAGTACCTCA TCTTCNTCTC 61 TGGTTTAATC ACACTCACCA TGGTCTCGAG CTTCGAAGCC TGACTTGGCA GCAAAGAATT 121 GCGAGCAACC GCATTCTCAT ACAGCTTGAC TTTGCTGTAA TCCCTGTCAT GGCTCAAGAT 181 CTCGATACGA TGGTCGATCT TGTGGGCATC TGGCAACTGC ACAACCTTTT TCGCTTTGTA 241 GGAAGTTTTG AAGTCACATC GGTGATGGCC ACCTCCTTCA AGCAACAGAG CCATGTTAAC 301 ATCACCCATC AGAAACCCAT CACGCACGTA CATCATCTCA GTGGATGGTT CCCACTTCAA 361 AGTTTTCTTC TGCATAACTG GACCATTGGG GGGAAAGTTC TTGCCATCAA ACCGAATTTT 421 ATAGATAAAG CAGTCGCCTT CCATGCTGAT CTCGCTTCTC ACGTTGCAAA TGGCGTTGTC 481 TTCATAACTC ATGGTTCTTT CCCATGAATA CCCCTCGGGA AACGTCTGCT TGAAATAGTC 541 TGGTATATCG GCTGGATATT TAGTGAATGC TCTATTTCCG TACTGGAACG CATTTGTCAA 601 GATGTCGTAA GAAAAAGGGA GAGGTGCGCC TTCTTTCACT GTCAGGTTTA AAGTCTGTGT 661 CCCATCGTAA GGCTTTCCTT TTCCATCTCC TTCAATCACA AATGCATGAN CGTTTACATT 721 TCCTTCCATA TGCAGCTTGA TCTTCATTTC CTCTTTAATC ACACTCATGG GATCCCCTGA 781 NATTGNTCTT CTGCTTGAAG CCNTCATTTG NTGGNGGGAA TAAAATANTC TTGCTNNNCN 841 AANNNCACNN ANCAAANGGG NCNACACNNC NNNNNNNCCC NAANNNTNNN NTNNNGNTTN 901 NNGNNNGTNN NNNNNNNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert mid(Mermaid 3') Sequence file: RDB15780_A8D8i.seq
>D04498J8_A8D8_3_SV40polyA-R2_B02_05_ABI24.ab1 1 NNNNNNNNNN NNNCNNNNAA NNNGTTAACA ACAANNATTG CATTCNTTTT ATGTTTCAGG 61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG 121 CTGATTATGA TCTAGATTAG GAATGAGCTA CTGCATCTTC TACCTGCTCA GTAATGTTGC 181 CTTCGGTTTT CCTGACGAGG CGATGGCCGA TGTAATGGTC TCCTGGCATT TCAAGAATCT 241 CTTTTGCCGC CTTGTAAGTA GTCTTGAATT GGCATTTGTG ATTGCCGCCT CCTTCAAGTT 301 TTAGGTACAT CGTAACATCA CCCTTCAGAA CTCCGTCGCT GGCAGTAATT TTCTCGGTTG 361 ATGGCTCCCA ATCAACACTT TGGTTTTGCA TGATAGGACC ATCGGCAGGA AAGTTAACCC 421 CAGTAAATTT GGATTTGTGG TAGAAGGTGT TTCCTCTAAG GCTTATATGC GCACTGACTG 481 AAGCGGACCC ACCATCTTCG AACTCCAACG ACCTTTCCCA TGACAGGCCT TCAGGAAATG 541 CTTGTTTGAA ATAGTCTGGT ATCTCTTCTG GATATTTAGT AAATACTCTG TGGCCGTAAC 601 AGAACACGTG TGACACTAAG TCAAACGCGA AAGGCATTGG CCCGCCCTCG GCCATTGTGA 661 CGCGTAGTGT CATCTCTTGA TGTCCCTCGT AAGGTCTGCC TGTGCCTTCA CCTTCNATTG 721 TGAACTCATG CCCATTGACG GANCCGTCCA TGTAGTACCT CATCTTCNTC TCTGGTTTAA 781 TCACACTCAC CATGGTCTCG AGCTTCGAAG CCTGANTTGN CAGCAAAGAA TTGCGAGCAA 841 CCGCATTCTC ATACAGCTTG ANTTTGCTGT AATCCCTGTC ATGNNTCAAG ATCTCGATAN 901 NATGGTCGAT CTTGTGGNNA TCTNGNNACN NNNCNACCTT TTNNNCTTTG NNNGNNNTTT 961 TGAANTCNCN TCNGNNANNG NCNNNNNNNT NNNNNCNNNN NANCNNTGNN NNCNTCACCN 1021 NNNNNAANNN NNNNNNN //
Primer: cmlc2pro_R (Pr0644) Region: insert 5'(cmlc2 pro),Tol2 1 Sequence file: RDB15780_A8D8j.seq
>D04498J8_A8D8_3_cmlc2pro_R_G03_21_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNCN NTGCCTGATT TTTATNNCTA TTGAATCAAT CATTTTAAAG 61 AATGTTAAGT CTGCTGATTT GGAGAGCTCA TTTACATTTA TTTTGAATGT CTCTTATTTA 121 ACACAACTGA TTTAAGCTTA TCGATACCGT CGACCTCGAG GGGGGGCCCA TCTGGCCTGT 181 GTTTCAGACA CCAGGGAGTC TCTGCTCACG TTTCCTGCTA TTTGCAGCCT CTCTATCAAG 241 ACTAATACAC CTCTTCCCGC ATCGGCTGCC TGTGAGAGGC TTTTCAGCAC TGCAGGATTG 301 CTTTTCAGCC CCAAAAGAGC TAGGCTTGAC ACTAACAATT TTGAGAATCA GCTTCTACTG 361 AAGTTAAATC TGAGGTTTTA CAACTTTGAG TAGCGTGTAC TGGCATTAGA TTGTCTGTCT 421 TATAGTTTGA TAATTAAATA CAAACAGTTC TAAAGCAGGA TAAAACCTTG TATGCATTTC 481 ATTTAATGTT TTTTGAGATT AAAAGCTTAA ACAAGAATCT CTAGTTTTCT TTCTTGCTTT 541 TACTTTTACT TCCTTAATAC TCAAGTACAA TTTTAATGGA GTACTTTTTT ACTTTTACTC 601 AAGTAAGATT CTAGCCAGAT ACTTTTACTT TTAATTGAGT AAAATTTTCC CTAAGTACTT 661 GTACTTTCAC TTGAGTAAAA TTTTTGAGTA CTTTTTACAC CTCTGTCAAG AACTCCTGGA 721 CAAACCTCTG ACCTGTGTGG AACAGAGTGG ATATGGGTGT CTGAACAGAT ATTCACGTCT 781 TTTGCAGATC AGANGGCATT TCTGGTGTTG TGCAATGACC TGGGTCCNAC ANGTAGGCGT 841 ACTCGTATCC NAGAGCATTG TTGGANAGGA NGTAGTCTCC ATTCCTATAA AGAGGAANNA 901 ATGGNACCNT GTANTANCCG TCATTGTGGN CAATTNNTGC NNTGGNACGT GGGTAGATGG 961 NNNGGGGNNG NTGANGANTT CTNANCNNGC NCTCNAANNN GCTANAAATG ANGTNNNNNN 1021 NNNN //
Primer: RDB15263#2 (Pr0525) Region: insert mid(Mermaid mid) Sequence file: RDB15780_A8D8k.seq
>15780_15780_B3CmF_RDB15263#2_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNA TCCTCNTGNT CATTGATCTC AGTCTTCCNG GAAAAAGTGA 61 ATCTTCACAA TCCTTTTATG ACGGGNTGGC TTTGGCTCTT TCTTGTTATT TCATGCTGGA 121 TTTAGGATTA AGGATATTTG CCTACGGGCC CAAGAATTTC TTCACCAACC CCTGGGAGGT 181 TGCTGATGGT TTGATTATCG TTGTCACATT CGTCGTCACG ATATTTTACA CTGTGTTAGA 241 TGAATACGTG CAAGAAACAG GAGCCGATGG TTTGGGGCAA TTGGTTGTGT TGGCCCGTTT 301 GCTGCGTGTG GTTCGATTAG CAAGAATATT TTATTCCCAC CAACAAATGA AGGCTTCAAG 361 CAGAAGAACA ATATCAGGGG ATCCCATGAG TGTGATTAAA GAGGAAATGA AGATCAAGCT 421 GCATATGGAA GGAAATGTAA ACGGTCATGC ATTTGTGATT GAAGGAGATG GAAAAGGAAA 481 GCCTTACGAT GGGACACAGA CTTTAAACCT GACAGTGAAA GAAGGCGCAC CTCTCCCTTT 541 TTCTTACGAC ATCTTGACAA ATGCGTTCCA GTACGGAAAT AGAGCATTCA CTAAATATCC 601 AGCCGATATA CCAGACTATT TCAAGCAGAC GTTTCCCGAN GGGTATTCAT GGGAAAGAAN 661 CATGANTTAT GAANNNNACG NCNTTTGCAA CGTGANANNC NANNNCNNNA NNNNANNNNN 721 NNNNN //
Primer: mUGK-B_F (Pr1311) Region: insert mid(Mermaid mid) Sequence file: RDB15780_A8D8l.seq
>15780_15780_B3CmF_mUGK-B_F_F01_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNGCNNN NNNNAGCTGT ATGAGAATGC GGTTGCTCGC 61 AATTCTTTGC TGCCAAGTCA GGCTTCGAAG CTCGAGACCA TGGTGAGTGT GATTAAACCA 121 GAGATGAAGA TGAGGTACTA CATGGACGGC TCCGTCAATG GGCATGAGTT CACAATTGAA 181 GGTGAAGGCA CAGGCAGACC TTACGAGGGA CATCAAGAGA TGACACTACG CGTCACAATG 241 GCCGAGGGCG GGCCAATGCC TTTCGCGTTT GACTTAGTGT CACACGTGTT CTGTTACGGC 301 CACAGAGTAT TTACTAAATA TCCAGAAGAG ATACCAGACT ATTTCAAACA AGCATTTCCT 361 GAAGGCCTGT CATGGGAAAG GTCGTTGGAG TTCGAAGATG GTGGGTCCGC TTCAGTCAGT 421 GCGCATATAA GCCTTAGAGG AAACACCTTC TACCACAAAT CCAAATTTAC TGGGGTTAAC 481 TTTCCTGCCG ATGGTCCTAT CATGCAAAAC CAAAGTGTTG ATTGGGAGCC ATNNNNNNNA 541 GAAAATTACT GCCAGCGACG GAGTTCTGAA GGGTGATGTT ACNATGTANN NNNNACTTGA 601 ANNNNNCGGC AATCACAAAT GCNANNTCAN GANTACTTAC NNNNNGGCAA AAGAGANTCN 661 TGAAANNNNC ANNNAGANNN NNNACATCNN NNNNCNCCNC GTCANGNAAA ACCNNANNGN 721 NAACNTTNNN NNNANCNNGN NNNANNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Tsutsui, H., Visualizing voltage dynamics in zebrafish heart. J. Physiol. 588 (Pt 12): 2017-2021 (2010). PMID 20421282. [link to RRC of NBRP]

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