Resource data sheet
Prev.

ptol2/cmlc2-mermaid

Catalog number RDB15780
Resource name ptol2/cmlc2-mermaid
Vector backbone Plasmid
Depositor Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
Tsutsui H, Higashijima SI, Miyawaki A, Okamura Y. (2010) Visualizing voltage dynamics in zebrafish heart. J. Physiol., 588 (Pt 12): 2017-2021.
4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Approval form by depositor [Word]
Remarks
Please obtain written approval of the depositor.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15780 ptol2/cmlc2-mermaid DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Tsutsui, H., Visualizing voltage dynamics in zebrafish heart. J. Physiol. 588 (Pt 12): 2017-2021 (2010). PMID 20421282.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dr. Miyawaki's resources (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15780_A8D8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: cmlc2pro_F RDB15780_A8D8a.seq
>D04498J8_A8D8_3_cmlc2pro_F_A02_02_ABI24.ab1
    1 ATATGAAACA TTGATAGAGT CCTCATACAT AAAGACTCCA TTAGTAAGCC AGTGACCCAG
   61 GAGCCCAGAC CAACAGCAAA GCAGACAGTG GATCCACTAG TTCTAGAGCG GCCGCATGGA
  121 GGGATTCGAC GGTTCAGATT TTAGTCCTCC AGCTGATTTA GTTGGCGTTG ACGGTGCAGT
  181 CATGCGGAAC GTCGTTGACG TCACGATAAA TGGTGACGTC ACTGCTCCGC CGAAAGCTGC
  241 GCCAAGAAAA AGTGAATCGG TAAAGAAAGT TCATTGGAAT GATGTAGACC AAGGACCGAG
  301 TGAAAAACCA GAGACAAGAC AGGAGGAACG AATAGATATA CCCGAGATTT CAGGTCTATG
  361 GTGGGGCGAG AATGAACATG GAGTGGACGA TGGGAGAATG GAGATACCTA CTACTGGTGT
  421 AGGTCGCGTC CAGTTTCGTG TCCGAGCAGT GATTGATCAT CTAGGGATGC GAGTCTTTGG
  481 AGTCTTCCTA ATTTTCTTGG ACATCATCCT CATGATCATT GATCTCAGTC TTCCAGGAAA
  541 AAGTGAATCT TCACAATCCT TTTATGACGG GATGGCTTTG GCTCTTTCTT GTTATTTCAT
  601 GCTGGATTTA GGATTAAGGA TATTTGCCTA CGGGCCCAAG AATTTCTTCA CCAACCCCTG
  661 GGAGGTTGCT GATGGTTTGA TTATCGTTGT CACATTCGTC GTCACGATAT TTTACACTGT
  721 GTTAGATGAA TACGTGCAAG AAACAGGAGC CGATGGTTTG GGGCAATTGG TTGTGTTGGC
  781 CCGTTTGCTG CGTGTGGTTC GATTAGCAAG AATATTTTAT TCCCACCAAC AAATGAAGGC
  841 TTCAAGCAGA AGAACAATAT CAGGGGATCC CATGAGTGTG ATTAAAGAGG AAATGAAGAT
  901 CAAGCTGCAT ATGGAAGGAA ATGTAAACGG TCATGCATTT GTGATTGAAG GAGATGGAAA
  961 AGGAAAGCCT TACGATGGGA CACAGACTTT AAACCTGACA GTGAAAGAAG GCGCACCTCT
 1021 CCCTTTTTCT TACGACATCT TGACAAATGC GTTTCCAGTA CGGAAATAGA GCATTCACTA
 1081 AAATATCAGC GATATACAGA CTATTTTCAA GCAGACGGTT TCCGAGGGGT ATTCATGGAA
1141 AGATCATGAG TAATGAGAAC AACGCCATTT TGCAACG
//
Primer: mKO2-N_R RDB15780_A8D8b.seq
>D04498J8_A8D8_3_mK02-N_R_B03_06_ABI24.ab1
    1 CCTTTCATGT GACTCATGCC CATTGACGGA GCCGTCCATG TAGTACCTCA TCTTCATCTC
   61 TGGTTTAATC ACACTCACCA TGGTCTCGAG CTTCGAAGCC TGACTTGGCA GCAAAGAATT
  121 GCGAGCAACC GCATTCTCAT ACAGCTTGAC TTTGCTGTAA TCCCTGTCAT GGCTCAAGAT
  181 CTCGATACGA TGGTCGATCT TGTGGGCATC TGGCAACTGC ACAACCTTTT TCGCTTTGTA
  241 GGAAGTTTTG AAGTCACATC GGTGATGGCC ACCTCCTTCA AGCAACAGAG CCATGTTAAC
  301 ATCACCCATC AGAAACCCAT CACGCACGTA CATCATCTCA GTGGATGGTT CCCACTTCAA
  361 AGTTTTCTTC TGCATAACTG GACCATTGGG GGGAAAGTTC TTGCCATCAA ACCGAATTTT
  421 ATAGATAAAG CAGTCGCCTT CCATGCTGAT CTCGCTTCTC ACGTTGCAAA TGGCGTTGTC
  481 TTCATAACTC ATGGTTCTTT CCCATGAATA CCCCTCGGGA AACGTCTGCT TGAAATAGTC
  541 TGGTATATCG GCTGGATATT TAGTGAATGC TCTATTTCCG TACTGGAACG CATTTGTCAA
  601 GATGTCGTAA GAAAAAGGGA GAGGTGCGCC TTCTTTCACT GTCAGGTTTA AAGTCTGTGT
  661 CCCATCGTAA GGCTTTCCTT TTCCATCTCC TTCAATCACA AATGCATGAC CGTTTACATT
  721 TCCTTCCATA TGCAGCTTGA TCTTCATTTC CTCTTTAATC ACACTCATGG GATCCCCTGA
  781 TATTGTTCTT CTGCTTGAAG CCTTCATTTG TTGGTGGGAA TAAAATATTC TTGCTAATCG
  841 AACCACACGC AGCAAACGGG CCAACACAAC CAATTGCCCC AAACCATCGG CTCCTGTTTC
  901 TTGCACGTAT TCATCTAACA CAGTGTAAAA ATATCGTGAC GACGATGTGA CACGATATCA
  961 AACCATCAGC ACCTCCCAGG ATGTGAAGAA ATCTGGGCCC GTAGCAATAT CTATCTATTC
 1021 AGCATGAATA CAGGACGAGC AGCATTCGTC ATAAGATGAG ATCACTTTTC TGGAGACTGA
1081 ATCAGAC
//
Primer: SV40polyA_R2 RDB15780_A8D8c.seq
>D04498J8_A8D8_3_SV40polyA-R2_B02_05_ABI24.ab1
    1 ACTTATAAGC CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG
   61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
  121 CTGATTATGA TCTAGATTAG GAATGAGCTA CTGCATCTTC TACCTGCTCA GTAATGTTGC
  181 CTTCGGTTTT CCTGACGAGG CGATGGCCGA TGTAATGGTC TCCTGGCATT TCAAGAATCT
  241 CTTTTGCCGC CTTGTAAGTA GTCTTGAATT GGCATTTGTG ATTGCCGCCT CCTTCAAGTT
  301 TTAGGTACAT CGTAACATCA CCCTTCAGAA CTCCGTCGCT GGCAGTAATT TTCTCGGTTG
  361 ATGGCTCCCA ATCAACACTT TGGTTTTGCA TGATAGGACC ATCGGCAGGA AAGTTAACCC
  421 CAGTAAATTT GGATTTGTGG TAGAAGGTGT TTCCTCTAAG GCTTATATGC GCACTGACTG
  481 AAGCGGACCC ACCATCTTCG AACTCCAACG ACCTTTCCCA TGACAGGCCT TCAGGAAATG
  541 CTTGTTTGAA ATAGTCTGGT ATCTCTTCTG GATATTTAGT AAATACTCTG TGGCCGTAAC
  601 AGAACACGTG TGACACTAAG TCAAACGCGA AAGGCATTGG CCCGCCCTCG GCCATTGTGA
  661 CGCGTAGTGT CATCTCTTGA TGTCCCTCGT AAGGTCTGCC TGTGCCTTCA CCTTCAATTG
  721 TGAACTCATG CCCATTGACG GAGCCGTCCA TGTAGTACCT CATCTTCATC TCTGGTTTAA
  781 TCACACTCAC CATGGTCTCG AGCTTCGAAG CCTGACTTGG CAGCAAAGAA TTGCGAGCAA
  841 CCGCATTCTC ATACAGCTTG ACTTTGCTGT AATCCCTGTC ATGGCTCAAG ATCTCGATAC
  901 GATGGTCGAT CTTGTGGGCA TCTGGCAACT GCACAACCTT TTTCGCTTTG TAGGAAGTTT
  961 TGAAGTCACA TCGGTGATGG CCACCTCCTT TCAAGCAACA GAGCCATGTT AACATCACCC
 1021 ATCAGAAACC CATCACGCAC GTACATCATC TCAGTGGGAT GGTTCCCACT TTCAAAGTTT
 1081 TCTTCTGCAT ACCTGACATT GGGGGGGAAA GTTCTTGCCA TCACCGAATT TATAGATTAA
1141 GCAGTTCGGC TTCATGCCTT GAATTCTCCT CGCTTTCCTC TACACACCG
//
Primer: cmlc2pro_R RDB15780_A8D8d.seq
>D04498J8_A8D8_3_cmlc2pro_R_G03_21_ABI24.ab1
    1 GATCTAAGTT AAAACTGGCT ATGCCTGATT TTTATCACTA TTGAATCAAT CATTTTAAAG
   61 AATGTTAAGT CTGCTGATTT GGAGAGCTCA TTTACATTTA TTTTGAATGT CTCTTATTTA
  121 ACACAACTGA TTTAAGCTTA TCGATACCGT CGACCTCGAG GGGGGGCCCA TCTGGCCTGT
  181 GTTTCAGACA CCAGGGAGTC TCTGCTCACG TTTCCTGCTA TTTGCAGCCT CTCTATCAAG
  241 ACTAATACAC CTCTTCCCGC ATCGGCTGCC TGTGAGAGGC TTTTCAGCAC TGCAGGATTG
  301 CTTTTCAGCC CCAAAAGAGC TAGGCTTGAC ACTAACAATT TTGAGAATCA GCTTCTACTG
  361 AAGTTAAATC TGAGGTTTTA CAACTTTGAG TAGCGTGTAC TGGCATTAGA TTGTCTGTCT
  421 TATAGTTTGA TAATTAAATA CAAACAGTTC TAAAGCAGGA TAAAACCTTG TATGCATTTC
  481 ATTTAATGTT TTTTGAGATT AAAAGCTTAA ACAAGAATCT CTAGTTTTCT TTCTTGCTTT
  541 TACTTTTACT TCCTTAATAC TCAAGTACAA TTTTAATGGA GTACTTTTTT ACTTTTACTC
  601 AAGTAAGATT CTAGCCAGAT ACTTTTACTT TTAATTGAGT AAAATTTTCC CTAAGTACTT
  661 GTACTTTCAC TTGAGTAAAA TTTTTGAGTA CTTTTTACAC CTCTGTCAAG AACTCCTGGA
  721 CAAACCTCTG ACCTGTGTGG AACAGAGTGG ATATGGGTGT CTGAACAGAT ATTCACGTCT
  781 TTTGCAGATC AGAGGGCATT TCTGGTGTTG TGCAATGACC TGGGTCCAAC AGGTAGGCGT
  841 ACTCGTATCC AAGAGCATTG TTGGACAGGA GGTAGTCTCC ATTCCTATAA AGAGGAAGGA
  901 ATGGCACCAT GTAGTAGCCG TCATTGTGGC CAATTGGTGC ATTGGTACGT GGGTAGATGG
  961 ACCGGGGAGG CTGATGAGTT CTTAGCCAGC GCTCAAAGAT GCTAGAAATG ATGTAAGAAC
 1021 AATATGCAAG ATGAAGTTAG ATAAGATAAA AATTTAAACA ATAATTGCAT TATGTTTTGT
 1081 GTTTCTATTC ACAAATAATA TCTCACGCAG TTAAAGCACA GGATATTTTT GTTCGTTGGC
1141 CTCTGGCCAG GGCCAACCCA A
//
Primer: mKOk-C_F RDB15780_A8D8e.seq
>D04498J8_A8D8_3_mKOk-C_F_H03_24_ABI24.ab1
    1 TGGAAGGCCA AGGGAGACAT TACATCGGCC ATCGCCTCGT CAGGAAAACC GAAGGCAACA
   61 TTACTGAGCA GGTAGAAGAT GCAGTAGCTC ATTCCTAATC TAGATCATAA TCAGCCATAC
  121 CACATTTGTA GAGGTTTTAC TTGCTTTAAA AAACCTCCCA CACCTCCCCC TGAACCTGAA
  181 ACATAAAATG AATGCAATTG TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA
  241 ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG
  301 TGGTTTGTCC AAACTCATCA ATGTATGCGG CCGCACTAGT CTCGAGTCTA GAGATCTGCG
  361 AAGATACGGC CACGGGTGCT CTTGATCCTG TGGCTGATTT TGGACTGTGC TGCTCGCAGC
  421 TGCTGATGAA TCACATACTT CCTCCATTTT CTTCCACTGA TTGACTGTTA TAATTTCCCT
  481 AATTTCCAGG TCAAGGTGCT GTGCATTGTG GTAATAGATG TGACATGACG TCACTTCCAA
  541 AGGACCAATG AACATGTCTG ACCAATTTCA TATAATGTGA AAACGATTTT CATAGGCAGA
  601 ATAAATAACA TTTAAATTAA ACTGGGCATC AGCGCAATTC AATTGGTTTG GTAATAGCAA
  661 GGGAAAATAG AATGAAGTGA TCTCCAAAAA ATAAGTACTT TTTGACTGTA AATAAAATTG
  721 TAAGGAGTAA AAAGTACTTT TTTTTCTAAA AAAATGTAAT TAAGTAAAAG TAAAAGTATT
  781 GATTTTTAAT TGTACTCAAG TAAAGTAAAA ATCCCCAAAA ATAATACTTA AGTACAGTAA
  841 TCAAGTAAAA TTACTCAAGT ACTTTACACC TCTGGTTCTT GACCCCCTAC CTTCAGCAAG
  901 CCCAGCAGAT CCACTAGGGC CGCCACCGCG GTGGAGCTCC AGCTTTTGTT CCCTTTAGTG
  961 AGGGTTAATT GCGCGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA
 1021 TCCGCTCACA ATTCCACACA ACAATACGAG CCCGGAAGCA TAAAGTGTAA AGCCTTGGGG
 1081 GTGCCTTAAT GAGTGAGCTA ACTCCACATT TAATTGCGTT TGCGCTCACT GACCGGCTTC
1141 AGTCCGAAAA CTGGTCGTGC CAAGCTGCAT TAATTGAAAT TCTGGGC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Tsutsui, H., Visualizing voltage dynamics in zebrafish heart. J. Physiol. 588 (Pt 12): 2017-2021 (2010). PMID 20421282.

2019.08.08

GNP_filter3_RDBDEP_html_190432.pl