RDB15780 - ptol2/cmlc2-mermaid

Catalog number	RDB15780
Resource name	ptol2/cmlc2-mermaid
Vector backbone	Plasmid
Depositor\|Developer	Miyawaki, Atsushi \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Tsutsui H, Higashijima SI, Miyawaki A, Okamura Y. (2010) Visualizing voltage dynamics in zebrafish heart. J. Physiol., 588 (Pt 12): 2017-2021. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Additional terms and conditions for distribution	The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki

Distribution information (domestic users)

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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Tsutsui H, Higashijima SI, Miyawaki A, Okamura Y. (2010) Visualizing voltage dynamics in zebrafish heart. J. Physiol., 588 (Pt 12): 2017-2021. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
付加的提供条件	Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15780	ptol2/cmlc2-mermaid	DNA solution

original	Tsutsui, H., Visualizing voltage dynamics in zebrafish heart. J. Physiol. 588 (Pt 12): 2017-2021 (2010). PMID 20421282.

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: cmlc2pro_F Region: cmlc2 promoter, insert 5' Sequence file: RDB15780_A8D8a.seq
>D04498J8_A8D8_3_cmlc2pro_F_A02_02_ABI24.ab1 1 ATATGAAACA TTGATAGAGT CCTCATACAT AAAGACTCCA TTAGTAAGCC AGTGACCCAG 61 GAGCCCAGAC CAACAGCAAA GCAGACAGTG GATCCACTAG TTCTAGAGCG GCCGCATGGA 121 GGGATTCGAC GGTTCAGATT TTAGTCCTCC AGCTGATTTA GTTGGCGTTG ACGGTGCAGT 181 CATGCGGAAC GTCGTTGACG TCACGATAAA TGGTGACGTC ACTGCTCCGC CGAAAGCTGC 241 GCCAAGAAAA AGTGAATCGG TAAAGAAAGT TCATTGGAAT GATGTAGACC AAGGACCGAG 301 TGAAAAACCA GAGACAAGAC AGGAGGAACG AATAGATATA CCCGAGATTT CAGGTCTATG 361 GTGGGGCGAG AATGAACATG GAGTGGACGA TGGGAGAATG GAGATACCTA CTACTGGTGT 421 AGGTCGCGTC CAGTTTCGTG TCCGAGCAGT GATTGATCAT CTAGGGATGC GAGTCTTTGG 481 AGTCTTCCTA ATTTTCTTGG ACATCATCCT CATGATCATT GATCTCAGTC TTCCAGGAAA 541 AAGTGAATCT TCACAATCCT TTTATGACGG GATGGCTTTG GCTCTTTCTT GTTATTTCAT 601 GCTGGATTTA GGATTAAGGA TATTTGCCTA CGGGCCCAAG AATTTCTTCA CCAACCCCTG 661 GGAGGTTGCT GATGGTTTGA TTATCGTTGT CACATTCGTC GTCACGATAT TTTACACTGT 721 GTTAGATGAA TACGTGCAAG AAACAGGAGC CGATGGTTTG GGGCAATTGG TTGTGTTGGC 781 CCGTTTGCTG CGTGTGGTTC GATTAGCAAG AATATTTTAT TCCCACCAAC AAATGAAGGC 841 TTCAAGCAGA AGAACAATAT CAGGGGATCC CATGAGTGTG ATTAAAGAGG AAATGAAGAT 901 CAAGCTGCAT ATGGAAGGAA ATGTAAACGG TCATGCATTT GTGATTGAAG GAGATGGAAA 961 AGGAAAGCCT TACGATGGGA CACAGACTTT AAACCTGACA GTGAAAGAAG GCGCACCTCT 1021 CCCTTTTTCT TACGACATCT TGACAAATGC GTTTCCAGTA CGGAAATAGA GCATTCACTA 1081 AAATATCAGC GATATACAGA CTATTTTCAA GCAGACGGTT TCCGAGGGGT ATTCATGGAA 1141 AGATCATGAG TAATGAGAAC AACGCCATTT TGCAACG //
Primer: mKO2-N_R Region: insert mid Sequence file: RDB15780_A8D8b.seq
>D04498J8_A8D8_3_mK02-N_R_B03_06_ABI24.ab1 1 CCTTTCATGT GACTCATGCC CATTGACGGA GCCGTCCATG TAGTACCTCA TCTTCATCTC 61 TGGTTTAATC ACACTCACCA TGGTCTCGAG CTTCGAAGCC TGACTTGGCA GCAAAGAATT 121 GCGAGCAACC GCATTCTCAT ACAGCTTGAC TTTGCTGTAA TCCCTGTCAT GGCTCAAGAT 181 CTCGATACGA TGGTCGATCT TGTGGGCATC TGGCAACTGC ACAACCTTTT TCGCTTTGTA 241 GGAAGTTTTG AAGTCACATC GGTGATGGCC ACCTCCTTCA AGCAACAGAG CCATGTTAAC 301 ATCACCCATC AGAAACCCAT CACGCACGTA CATCATCTCA GTGGATGGTT CCCACTTCAA 361 AGTTTTCTTC TGCATAACTG GACCATTGGG GGGAAAGTTC TTGCCATCAA ACCGAATTTT 421 ATAGATAAAG CAGTCGCCTT CCATGCTGAT CTCGCTTCTC ACGTTGCAAA TGGCGTTGTC 481 TTCATAACTC ATGGTTCTTT CCCATGAATA CCCCTCGGGA AACGTCTGCT TGAAATAGTC 541 TGGTATATCG GCTGGATATT TAGTGAATGC TCTATTTCCG TACTGGAACG CATTTGTCAA 601 GATGTCGTAA GAAAAAGGGA GAGGTGCGCC TTCTTTCACT GTCAGGTTTA AAGTCTGTGT 661 CCCATCGTAA GGCTTTCCTT TTCCATCTCC TTCAATCACA AATGCATGAC CGTTTACATT 721 TCCTTCCATA TGCAGCTTGA TCTTCATTTC CTCTTTAATC ACACTCATGG GATCCCCTGA 781 TATTGTTCTT CTGCTTGAAG CCTTCATTTG TTGGTGGGAA TAAAATATTC TTGCTAATCG 841 AACCACACGC AGCAAACGGG CCAACACAAC CAATTGCCCC AAACCATCGG CTCCTGTTTC 901 TTGCACGTAT TCATCTAACA CAGTGTAAAA ATATCGTGAC GACGATGTGA CACGATATCA 961 AACCATCAGC ACCTCCCAGG ATGTGAAGAA ATCTGGGCCC GTAGCAATAT CTATCTATTC 1021 AGCATGAATA CAGGACGAGC AGCATTCGTC ATAAGATGAG ATCACTTTTC TGGAGACTGA 1081 ATCAGAC //
Primer: SV40polyA_R2 Region: SV40 poly(A) signal, insert 3' Sequence file: RDB15780_A8D8c.seq
>D04498J8_A8D8_3_SV40polyA-R2_B02_05_ABI24.ab1 1 ACTTATAAGC CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG 61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG 121 CTGATTATGA TCTAGATTAG GAATGAGCTA CTGCATCTTC TACCTGCTCA GTAATGTTGC 181 CTTCGGTTTT CCTGACGAGG CGATGGCCGA TGTAATGGTC TCCTGGCATT TCAAGAATCT 241 CTTTTGCCGC CTTGTAAGTA GTCTTGAATT GGCATTTGTG ATTGCCGCCT CCTTCAAGTT 301 TTAGGTACAT CGTAACATCA CCCTTCAGAA CTCCGTCGCT GGCAGTAATT TTCTCGGTTG 361 ATGGCTCCCA ATCAACACTT TGGTTTTGCA TGATAGGACC ATCGGCAGGA AAGTTAACCC 421 CAGTAAATTT GGATTTGTGG TAGAAGGTGT TTCCTCTAAG GCTTATATGC GCACTGACTG 481 AAGCGGACCC ACCATCTTCG AACTCCAACG ACCTTTCCCA TGACAGGCCT TCAGGAAATG 541 CTTGTTTGAA ATAGTCTGGT ATCTCTTCTG GATATTTAGT AAATACTCTG TGGCCGTAAC 601 AGAACACGTG TGACACTAAG TCAAACGCGA AAGGCATTGG CCCGCCCTCG GCCATTGTGA 661 CGCGTAGTGT CATCTCTTGA TGTCCCTCGT AAGGTCTGCC TGTGCCTTCA CCTTCAATTG 721 TGAACTCATG CCCATTGACG GAGCCGTCCA TGTAGTACCT CATCTTCATC TCTGGTTTAA 781 TCACACTCAC CATGGTCTCG AGCTTCGAAG CCTGACTTGG CAGCAAAGAA TTGCGAGCAA 841 CCGCATTCTC ATACAGCTTG ACTTTGCTGT AATCCCTGTC ATGGCTCAAG ATCTCGATAC 901 GATGGTCGAT CTTGTGGGCA TCTGGCAACT GCACAACCTT TTTCGCTTTG TAGGAAGTTT 961 TGAAGTCACA TCGGTGATGG CCACCTCCTT TCAAGCAACA GAGCCATGTT AACATCACCC 1021 ATCAGAAACC CATCACGCAC GTACATCATC TCAGTGGGAT GGTTCCCACT TTCAAAGTTT 1081 TCTTCTGCAT ACCTGACATT GGGGGGGAAA GTTCTTGCCA TCACCGAATT TATAGATTAA 1141 GCAGTTCGGC TTCATGCCTT GAATTCTCCT CGCTTTCCTC TACACACCG //
Primer: cmlc2pro_R Region: Tol2 transposase recombinant sequence 1 Sequence file: RDB15780_A8D8d.seq
>D04498J8_A8D8_3_cmlc2pro_R_G03_21_ABI24.ab1 1 GATCTAAGTT AAAACTGGCT ATGCCTGATT TTTATCACTA TTGAATCAAT CATTTTAAAG 61 AATGTTAAGT CTGCTGATTT GGAGAGCTCA TTTACATTTA TTTTGAATGT CTCTTATTTA 121 ACACAACTGA TTTAAGCTTA TCGATACCGT CGACCTCGAG GGGGGGCCCA TCTGGCCTGT 181 GTTTCAGACA CCAGGGAGTC TCTGCTCACG TTTCCTGCTA TTTGCAGCCT CTCTATCAAG 241 ACTAATACAC CTCTTCCCGC ATCGGCTGCC TGTGAGAGGC TTTTCAGCAC TGCAGGATTG 301 CTTTTCAGCC CCAAAAGAGC TAGGCTTGAC ACTAACAATT TTGAGAATCA GCTTCTACTG 361 AAGTTAAATC TGAGGTTTTA CAACTTTGAG TAGCGTGTAC TGGCATTAGA TTGTCTGTCT 421 TATAGTTTGA TAATTAAATA CAAACAGTTC TAAAGCAGGA TAAAACCTTG TATGCATTTC 481 ATTTAATGTT TTTTGAGATT AAAAGCTTAA ACAAGAATCT CTAGTTTTCT TTCTTGCTTT 541 TACTTTTACT TCCTTAATAC TCAAGTACAA TTTTAATGGA GTACTTTTTT ACTTTTACTC 601 AAGTAAGATT CTAGCCAGAT ACTTTTACTT TTAATTGAGT AAAATTTTCC CTAAGTACTT 661 GTACTTTCAC TTGAGTAAAA TTTTTGAGTA CTTTTTACAC CTCTGTCAAG AACTCCTGGA 721 CAAACCTCTG ACCTGTGTGG AACAGAGTGG ATATGGGTGT CTGAACAGAT ATTCACGTCT 781 TTTGCAGATC AGAGGGCATT TCTGGTGTTG TGCAATGACC TGGGTCCAAC AGGTAGGCGT 841 ACTCGTATCC AAGAGCATTG TTGGACAGGA GGTAGTCTCC ATTCCTATAA AGAGGAAGGA 901 ATGGCACCAT GTAGTAGCCG TCATTGTGGC CAATTGGTGC ATTGGTACGT GGGTAGATGG 961 ACCGGGGAGG CTGATGAGTT CTTAGCCAGC GCTCAAAGAT GCTAGAAATG ATGTAAGAAC 1021 AATATGCAAG ATGAAGTTAG ATAAGATAAA AATTTAAACA ATAATTGCAT TATGTTTTGT 1081 GTTTCTATTC ACAAATAATA TCTCACGCAG TTAAAGCACA GGATATTTTT GTTCGTTGGC 1141 CTCTGGCCAG GGCCAACCCA A //
Primer: mKOk-C_F Region: deltaTol2 transposase recombinant sequence 2 Sequence file: RDB15780_A8D8e.seq
>D04498J8_A8D8_3_mKOk-C_F_H03_24_ABI24.ab1 1 TGGAAGGCCA AGGGAGACAT TACATCGGCC ATCGCCTCGT CAGGAAAACC GAAGGCAACA 61 TTACTGAGCA GGTAGAAGAT GCAGTAGCTC ATTCCTAATC TAGATCATAA TCAGCCATAC 121 CACATTTGTA GAGGTTTTAC TTGCTTTAAA AAACCTCCCA CACCTCCCCC TGAACCTGAA 181 ACATAAAATG AATGCAATTG TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA 241 ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG 301 TGGTTTGTCC AAACTCATCA ATGTATGCGG CCGCACTAGT CTCGAGTCTA GAGATCTGCG 361 AAGATACGGC CACGGGTGCT CTTGATCCTG TGGCTGATTT TGGACTGTGC TGCTCGCAGC 421 TGCTGATGAA TCACATACTT CCTCCATTTT CTTCCACTGA TTGACTGTTA TAATTTCCCT 481 AATTTCCAGG TCAAGGTGCT GTGCATTGTG GTAATAGATG TGACATGACG TCACTTCCAA 541 AGGACCAATG AACATGTCTG ACCAATTTCA TATAATGTGA AAACGATTTT CATAGGCAGA 601 ATAAATAACA TTTAAATTAA ACTGGGCATC AGCGCAATTC AATTGGTTTG GTAATAGCAA 661 GGGAAAATAG AATGAAGTGA TCTCCAAAAA ATAAGTACTT TTTGACTGTA AATAAAATTG 721 TAAGGAGTAA AAAGTACTTT TTTTTCTAAA AAAATGTAAT TAAGTAAAAG TAAAAGTATT 781 GATTTTTAAT TGTACTCAAG TAAAGTAAAA ATCCCCAAAA ATAATACTTA AGTACAGTAA 841 TCAAGTAAAA TTACTCAAGT ACTTTACACC TCTGGTTCTT GACCCCCTAC CTTCAGCAAG 901 CCCAGCAGAT CCACTAGGGC CGCCACCGCG GTGGAGCTCC AGCTTTTGTT CCCTTTAGTG 961 AGGGTTAATT GCGCGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA 1021 TCCGCTCACA ATTCCACACA ACAATACGAG CCCGGAAGCA TAAAGTGTAA AGCCTTGGGG 1081 GTGCCTTAAT GAGTGAGCTA ACTCCACATT TAATTGCGTT TGCGCTCACT GACCGGCTTC 1141 AGTCCGAAAA CTGGTCGTGC CAAGCTGCAT TAATTGAAAT TCTGGGC //