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Masato Kanemaki's Resource

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Catalog #Name of cloneRunning titlePublication
RDB08468pNHK60Expression clone for an auxin-based degron (AID) system, GFP-aid-NLS.Nishimura, K., An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods. 6(12), 917-922, (2009). PMID 19915560. (original).
RDB08469pMK106Expression clone for an auxin-based degron (AID) system, aid-CENPH.Nishimura, K., An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods. 6(12), 917-22, (2009). PMID 19915560. (original).
RDB08470pMK107Expression clone for an auxin-based degron (AID) system, CENPH-aid.Nishimura, K., An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods. 6(12), 917-22, (2009). PMID 19915560. (original).
RDB13917AAVS1 T2 CRISPR in pX330A CRISPR/Cas plasmid for making DNA dounle-strand break at the human AAVS1 locus.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original).
RDB13918pMK232 (AAVS1 CMV-OsTIR1-PURO)A donor vector for introducing CMV-OsTIR1 at the human AAVS1 locus.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original).
RDB13920pMK277 (mClover-NeoR)A plasmid for construction of C-terminal mClover tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original).
RDB13921pMK278 (mClover-Hygro)A plasmid for construction of C-terminal mClover tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original).
RDB13922pMK279 (mClover-Bsr)A plasmid for construction of C-terminal mClover tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original).
RDB13923pMK280 (mCherry2-NeoR)A plasmid for construction of C-terminal mCherry tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original).
RDB13924pMK281 (mCherry2-Hygro)A plasmid for construction of C-terminal mCherry tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original).
RDB13925pMK282 (mCherry2-Bsr)A plasmid for construction of C-terminal mCherry tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original).
RDB13926pMK283 (Stag-3FLAG-Neo)A plasmid for construction of C-terminal Stag-3FLAG tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original).
RDB13927pMK284 (Stag-FLAG-Hygro)A plasmid for construction of C-terminal Stag-3FLAG tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original).
RDB13928pMK285 (Stag-3FLAG-Bsr)A plasmid for construction of C-terminal Stag-3FLAG tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original).
RDB13929pMK286 (mAID-NeoR)A plasmid for construction of C-terminal mAID tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original).
RDB13930pMK287 (mAID-Hygro)A plasmid for construction of C-terminal mAID tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original).
RDB13931pMK288 (mAID-Bsr)A plasmid for construction of C-terminal mAID tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original).
RDB13932pMK289 (mAID-mClover-NeoR)A plasmid for construction of C-terminal mAID-mClover tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original).
RDB13933pMK290 (mAID-mClover-Hygro)A plasmid for construction of C-terminal mAID-mClover tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original).
RDB13934pMK291 (mAID-mClover-Bsr)A plasmid for construction of C-terminal mAID-mClover tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original).
RDB13935pMK292 (mAID-mCherry2-NeoR)A plasmid for construction of C-terminal mAID-mCherry tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original).
RDB13936pMK293 (mAID-mCherry2-Hygro)A plasmid for construction of C-terminal mAID-mCherry tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original).
RDB13937pMK294 (mAID-mCherry2-Bsr)A plasmid for construction of C-terminal mAID-mCherry tagging donors.Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. (original).
RDB16805pMK344 (Hygro-P2A-mAID)A plasmid for construction of N-terminal Hygro-P2A-mAID tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB16806pMK345 (Hygro-P2A-mAID-mClover)A plasmid for construction of N-terminal Hygro-P2A-mAID-mClover tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB16807pMK346 (Hygro-P2A-mAID-mCherry2)A plasmid for construction of N-terminal Hygro-P2A-mAID-mCherry2 tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB16808pMK347 (BSD-P2A-mAID)A plasmid for construction of N-terminal BSD-P2A-mAID tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB16809pMK348 (BSD-P2A-mAID-mClover)A plasmid for construction of N-terminal BSD-P2A-mAID-mClover tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB16810pMK349 (BSD-P2A-mAID-mCherry2)A plasmid for construction of N-terminal BSD-P2A-mAID-mCherry2 tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB16811pMK364 (CMV-OsTIR1-loxP-PURO-loxP)A donor vector for introducing CMV-OsTIR1 at the human AAVS1 locus.Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB16813pMK375 (Hygro-P2A-Stag-3FLAG)A plasmid for construction of N-terminal Hygro-P2A-Stag-3FLAG tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB16814pMK376 (Hygro-P2A-mClover)A plasmid for construction of N-terminal Hygro-P2A-mClover tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB16815pMK377 (Hygro-P2A-mCherry2)A plasmid for construction of N-terminal Hygro-P2A-mCherry2 tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB16816pMK388 (mClover-BSD)A plasmid for construction of C-terminal mClover-BSD tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB16817pMK389 (mCherry2-BSD)A plasmid for construction of C-terminal mCherry2-BSD tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB16818pMK390 (Stag-3FLAG-BSD)A plasmid for construction of C-terminal Stag-3FLAG-BSD tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB16819pMK391 (mAID-BSD)A plasmid for construction of C-terminal mAID-BSD tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB16820pMK392 (mAID-mClover-BSD)A plasmid for construction of C-terminal mAID-mClover-BSD tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB16821pMK393 (mAID-mCherry2-BSD)A plasmid for construction of C-terminal mAID-mCherry2-BSD tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB16822pMK394 (mAID-Nluc-Neo)A plasmid for construction of C-terminal mAID-Nluc-Neo tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB16823pMK401 (Hygro-P2A-mAID-Nluc)A plasmid for construction of N-terminal Hygro-P2A-mAID-Nluc tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB16824pMK407 (BSD-P2A-mAID-Nluc)A plasmid for construction of N-terminal BSD-P2A-mAID-Nluc tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB16825pMK408 (mAID-Nluc Hygro)A plasmid for construction of C-terminal mAID-Nluc Hygro tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB16826pMK409 (mAID-Nluc BSD)A plasmid for construction of C-terminal mAID-Nluc BSD tagging donors. Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. (original).
RDB18333pAY7TOL2 expression vector of OsTIR1(WT) and mAID-EGFP-NLS marker.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18334pAY15TOL2 expression vector of OsTIR1(F74G) and mAID-EGFP-NLS marker.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18335pAY19TOL2 expression vector of OsTIR1(F74A) and mAID-EGFP-NLS marker.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18337pMK381A donor vector for introducing CMV-OsTIR1 (F74G mutant) at the human AAVS1 locus.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18338pAAV-hSyn-OsTIR1(WT) Adeno-associated virus (AAV) expression vector of OsTIR1(wild type) and mAID-EGFP-NES marker with hSyn promoter.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18339pAAV-hSyn-OsTIR1(F74G) Adeno-associated virus (AAV) expression vector of OsTIR1(F74G) and mAID-EGFP-NES marker with hSyn promoter.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18340pAAV-hSyn-OsTIR1(F74A) Adeno-associated virus (AAV) expression vector of OsTIR1(F74A) and mAID-EGFP-NES marker with hSyn promoter.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18341pMK198A plasmid for generate yeast lines expressing OsTIR1(wild type) under the GAL promoter.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18342pMK419A plasmid for generate yeast lines expressing OsTIR1(F74G) under the GAL promoter.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18343pMK425A plasmid for generate yeast lines expressing OsTIR1(F74A) under the GAL promoter.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18344pMK262Donor plasmid to generate RAD21-mAID degron cell lines with mClover as a marker, neomycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18345pMK265Donor plasmid to generate RAD21-mAID degron cell lines with mClover as a marker, hygromycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18346RAD21 C-tag CIRPSRExpression vector of Cas9 and gRNA for a RAD21 tagging donor.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18347pMK233A donor vector for introducing CMV-AtAFB2 at the human AAVS1 locus.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18348pMK296Donor plasmid to generate DHC1-mAID degron cell lines with mClover as a marker, neomycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18349pMK297Donor plasmid to generate DHC1-mAID degron cell lines with mClover as a marker, hygromycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18350DHC1-mIAA7 NeoDonor plasmid to generate DHC1-mlAA7 degron cell lines with mClover as a marker, neomycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18351DHC1-mIAA7 HygroDonor plasmid to generate DHC1-mlAA7 degron cell lines with mClover as a marker, hygromycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18352DHC1 CRISPRExpression vector of Cas9 and gRNA for a DHC1 tagging donor.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18353CTCF-mAC donor (Neo) Donor plasmid to generate CTCF-mAID degron cell lines with mClover as a marker, neomycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18354CTCF-mAC donor (Hygro) Donor plasmid to generate CTCF-mAID degron cell lines with mClover as a marker, hygromycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18355CTCF-C CRISPRExpression vector of Cas9 and gRNA for a CTCF tagging donor.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18356SMC2-mAC donor (Hygro) Donor plasmid to generate SMC2-mAID degron cell lines with mClover as a marker, hygromycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18357SMC2 CRISPRExpression vector of Cas9 and gRNA for a SMC2 tagging donor.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18358mAID-BRD4 donorDonor plasmid to generate BRD4-mAID degron cell lines, hygromycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18359BRD4-N CRISPRExpression vector of Cas9 and gRNA for a BRD4 tagging donor.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18360pMK321Donor plasmid to generate TOP2A-mAID degron cell lines with mClover as a marker, neomycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18361pMK322Donor plasmid to generate TOP2A-mAID degron cell lines with mClover as a marker, hygromycin resistance.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18362pMK312Expression vector of Cas9 and gRNA for a TOP2A tagging donor.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18363pMK427TOL2 expression vector of OsTIR1(WT) and mAID-EGFP-Nluc marker.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).
RDB18364pMK411TOL2 expression vector of OsTIR1(F74G) and mAID-EGFP-Nluc marker.Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). (original).

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2021.03.26

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