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pMK347 (BSD-P2A-mAID)

A plasmid for construction of N-terminal BSD-P2A-mAID tagging donors.

Catalog number RDB16808
Resource name pMK347 (BSD-P2A-mAID)
Clone info. A plasmid for construction of N-terminal BSD-P2A-mAID tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA
Depositor Kanemaki, Masato |
 
Other clones in our bank Arabidopsis thaliana AXR3 (KEGG orthology K14484) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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RDB16808 pMK347 (BSD-P2A-mAID) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16808_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 RDB16808_A8Lpa.seq
>D06157A4_A8LP_2_pUC-M13_-100_A02_02_ABI24.ab1
    1 GTTCGTTCAG GCCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA
   61 ACGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGG TACCGGGCCC
  121 CCCCTCGAGG TCGACGGTAT CGATAAGCTT GATATCGCCA CCATGGCCAA GCCTTTGTCT
  181 CAAGAAGAAT CCACCCTCAT TGAAAGAGCA ACGGCTACAA TCAACAGCAT CCCCATCTCT
  241 GAAGACTACA GCGTCGCCAG CGCAGCTCTC TCTAGCGACG GCCGCATCTT CACTGGTGTC
  301 AATGTATATC ATTTTACTGG GGGACCTTGT GCAGAACTCG TGGTGCTGGG CACTGCTGCT
  361 GCTGCGGCAG CTGGCAACCT GACTTGTATC GTCGCGATCG GAAATGAGAA CAGGGGCATC
  421 TTGAGCCCCT GCGGACGGTG CCGACAGGTG CTTCTCGATC TGCATCCTGG GATCAAAGCC
  481 ATAGTGAAGG ACAGTGATGG ACAGCCGACG GCAGTTGGGA TTCGTGAATT GCTGCCCTCT
  541 GGTTATGTGT GGGAGGGCTC AGGAAGCGGA GCTACTAACT TCAGCCTGCT GAAGCAGGCT
  601 GGAGACGTGG AGGAGAACCC TGGACCTAGA TCTAAGGAGA AGAGTGCTTG TCCTAAAGAT
  661 CCAGCCAAAC CTCCGGCCAA GGCACAAGTT GTGGGATGGC CACCGGTGAG ATCATACCGG
  721 AAGAACGTGA TGGTTTCCTG CCAAAAATCA AGCGGTGGCC CGGAGGCGGC GGCGTTCGTG
  781 AAGGTATCAA TGGACGGAGC ACCGTACTTG AGGAAAATCG ATTTGAGGAT GTATAAAGGC
  841 GCTGGTGCAG GCGCCGGATC CACTAGTTCT AGAGCGGCCG CCACCGCGGT GGAGCTCCAG
  901 CTTTTGTTCC CTTTAGTGAG GGTTAATTGC GCGCTTGGCG TAATCATGGG TCATAGCTGT
  961 TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGGAAGCATA
 1021 AAGTGTAAAG CCTGGGGGTG CCTTAAATGA GTGGAGCTTA ACTCACATAA ATGCCGTGCG
1081 CTCACTGCCG CTTCAGCTCG GACTGATCGA TTGGCACGAG TC
//
Primer: reverse RDB16808_A8Lpb.seq
>D06157A4_A8LP_2_reverse_B02_05_ABI24.ab1
    1 CAAAACAATT ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCACCGCGG TGGCGGCCGC
   61 TCTAGAACTA GTGGATCCGG CGCCTGCACC AGCGCCTTTA TACATCCTCA AATCGATTTT
  121 CCTCAAGTAC GGTGCTCCGT CCATTGATAC CTTCACGAAC GCCGCCGCCT CCGGGCCACC
  181 GCTTGATTTT TGGCAGGAAA CCATCACGTT CTTCCGGTAT GATCTCACCG GTGGCCATCC
  241 CACAACTTGT GCCTTGGCCG GAGGTTTGGC TGGATCTTTA GGACAAGCAC TCTTCTCCTT
  301 AGATCTAGGT CCAGGGTTCT CCTCCACGTC TCCAGCCTGC TTCAGCAGGC TGAAGTTAGT
  361 AGCTCCGCTT CCTGAGCCCT CCCACACATA ACCAGAGGGC AGCAATTCAC GAATCCCAAC
  421 TGCCGTCGGC TGTCCATCAC TGTCCTTCAC TATGGCTTTG ATCCCAGGAT GCAGATCGAG
  481 AAGCACCTGT CGGCACCGTC CGCAGGGGCT CAAGATGCCC CTGTTCTCAT TTCCGATCGC
  541 GACGATACAA GTCAGGTTGC CAGCTGCCGC AGCAGCAGCA GTGCCCAGCA CCACGAGTTC
  601 TGCACAAGGT CCCCCAGTAA AATGATATAC ATTGACACCA GTGAAGATGC GGCCGTCGCT
  661 AGAGAGAGCT GCGCTGGCGA CGCTGTAGTC TTCAGAGATG GGGATGCTGT TGATTGTAGC
  721 CGTTGCTCTT TCAATGAGGG TGGATTCTTC TTGAGACAAA GGCTTGGCCA TGGTGGCGAT
  781 ATCAAGCTTA TCGATACCGT CGACCTCGAG GGGGGGCCCG GTACCCAATT CGCCCTATAG
  841 TGAGTCGTAT TACGCGCGCT CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC
  901 TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCC TTTTCGCCAG CTGGCGTAAT
  961 AGCGAAGAGG CCCGCACCCG ATCGCCCCTT CCCAACAGTT GCGCAGCCCT GAATTGGCGA
 1021 ATGGAAATTT GGTAAGCGGT TAATATTTTG ATAAAATTTC GCGATAATTT TTGATAATTC
1081 CAGCTTCAAT TTTTTTTTAA ACCCAAGTAG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

2019.07.12

GNP_filter3_RDBDEP_html_190431.pl