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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK347 (BSD-P2A-mAID)

A plasmid for construction of N-terminal BSD-P2A-mAID tagging donors.

Catalog number RDB16808
Resource name pMK347 (BSD-P2A-mAID)
Clone info. A plasmid for construction of N-terminal BSD-P2A-mAID tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank
External Database
Arabidopsis thaliana AtIAA17
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
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          手続きの概要は、「提供申込みについて[link]」をご覧ください。

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          RDB16808 pMK347 (BSD-P2A-mAID) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pMK347 (BSD-P2A-mAID) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16808).

          Reference section:

          Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

          Tips

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16808_A8Lpp1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-M13_-100 (Pr0310)
          Region: T7 pro,insert 5'(BSD,P2A,mAID 5')
          Sequence file: RDB16808_A8Lpc.seq check
          >D06157A4_A8LP_2_pUC-M13_-100_A02_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNGGNANNG CCNGNNNTTT CCCNGTCACG ACGTTGTAAA
             61 ACGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGG TACCGGGCCC
            121 CCCCTCGAGG TCGACGGTAT CGATAAGCTT GATATCGCCA CCATGGCCAA GCCTTTGTCT
            181 CAAGAAGAAT CCACCCTCAT TGAAAGAGCA ACGGCTACAA TCAACAGCAT CCCCATCTCT
            241 GAAGACTACA GCGTCGCCAG CGCAGCTCTC TCTAGCGACG GCCGCATCTT CACTGGTGTC
            301 AATGTATATC ATTTTACTGG GGGACCTTGT GCAGAACTCG TGGTGCTGGG CACTGCTGCT
            361 GCTGCGGCAG CTGGCAACCT GACTTGTATC GTCGCGATCG GAAATGAGAA CAGGGGCATC
            421 TTGAGCCCCT GCGGACGGTG CCGACAGGTG CTTCTCGATC TGCATCCTGG GATCAAAGCC
            481 ATAGTGAAGG ACAGTGATGG ACAGCCGACG GCAGTTGGGA TTCGTGAATT GCTGCCCTCT
            541 GGTTATGTGT GGGAGGGCTC AGGAAGCGGA GCTACTAACT TCAGCCTGCT GAAGCAGGCT
            601 GGAGACGTGG AGGAGAACCC TGGACCTAGA TCTAAGGAGA AGAGTGCTTG TCCTAAAGAT
            661 CCAGCCAAAC CTCCGGCCAA GGCACAAGTT GTGGGATGGC CACCGGTGAG ATCATACCGG
            721 AAGAACGTGA TGGTTTCCTG CCNAAAATCA AGCGGNGGCC CGGANGCGGC GGCGTTCGTG
            781 AAGGTATCAA TGGACGGANC ACCGTACTTG ANGAAAATCN ATTTGAGGAT GTATAANGGC
            841 GCTGGNGCNN NCGCNNGNNN CNCTANTTCT AGAGCGGNCG CCNCNGNNGN GGANCTCCAG
          901 CTTTTGNTCC NNTTANNGAG GGTNANTTGN GCGCTNGNNN NNNNNNN
          //
          Primer: reverse (Pr0099)
          Region: insert 3'(Linker,mAID,P2A,BSD 3')
          Sequence file: RDB16808_A8Lpd.seq check
          >D06157A4_A8LP_2_reverse_B02_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN ANGGNNNNNN NNGCTGGAGC TCCNCCGCGG TGGCGGCCGC
             61 TCTAGAACTA GTGGATCCGG CGCCTGCACC AGCGCCTTTA TACATCCTCA AATCGATTTT
            121 CCTCAAGTAC GGTGCTCCGT CCATTGATAC CTTCACGAAC GCCGCCGCCT CCGGGCCACC
            181 GCTTGATTTT TGGCAGGAAA CCATCACGTT CTTCCGGTAT GATCTCACCG GTGGCCATCC
            241 CACAACTTGT GCCTTGGCCG GAGGTTTGGC TGGATCTTTA GGACAAGCAC TCTTCTCCTT
            301 AGATCTAGGT CCAGGGTTCT CCTCCACGTC TCCAGCCTGC TTCAGCAGGC TGAAGTTAGT
            361 AGCTCCGCTT CCTGAGCCCT CCCACACATA ACCAGAGGGC AGCAATTCAC GAATCCCAAC
            421 TGCCGTCGGC TGTCCATCAC TGTCCTTCAC TATGGCTTTG ATCCCAGGAT GCAGATCGAG
            481 AAGCACCTGT CGGCACCGTC CGCAGGGGCT CAAGATGCCC CTGTTCTCAT TTCCGATCGC
            541 GACGATACAA GTCAGGTTGC CAGCTGCCGC AGCAGCAGCA GTGCCCAGCA CCACGAGTTC
            601 TGCACAAGGT CCCCCAGTAA AATGATATAC ATTGACACCA GTGAAGATGC GGCCGTCGCT
            661 AGAGAGAGCT GCGCTGGCGA CGCTGTAGTC TTCAGAGATG GGGATGCTGT TGANTGTAGC
            721 CGTTGCTCTT TCAATGAGGG TGGANTCTTC TTGAGACAAA GGCTTGGCCA TGGTGGCGAT
            781 ATCAAGCTTA TCGATACCGT CGACCTCNAG GGGGGGCCCG GNANCCNATT CGCCCTATAG
            841 NGANTCGNAN TACGCGCGCT CACNGGNCGT CGTTTTACAA CGTCNNGNNN GGNNAAACCN
          901 TGGNGTTANC NNACTTAANC GCNNTNNNGC NNNTCCCCCN NTTNNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

          2024.03.26

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