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pMK278 (mClover-Hygro)

A plasmid for construction of C-terminal mClover tagging donors.

Catalog number RDB13921
Resource name pMK278 (mClover-Hygro)
Alternative name mClover-Hygro, 1000
Clone info. A plasmid for construction of C-terminal mClover tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor Kanemaki, Masato |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13921 pMK278 (mClover-Hygro) DNA solution

cDNA Request [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)
Featured content Genome Editing (English text)
Featured content Genome Editing (Japanese text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

References

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13921_A6B8p1_v2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB13921_A6B8a_2.seq
>D03070A05_A6B8_1_M13(-40)_B02_05.ab1
    1 CCGCCGGTAG CGCGCGTATA CGACTCACTA TAGGGCGAAT TGGAGCTCGG ATCCGGTGCA
   61 GGCGCCGCTA GCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG
  121 GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TCCGCGGCGA GGGCGAGGGC
  181 GATGCCACCA ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG
  241 CCCTGGCCCA CCCTCGTGAC CACCTTCGGC TACGGCGTGG CCTGCTTCAG CCGCTACCCC
  301 GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG
  361 CGCACCATCT CTTTCAAGGA CGACGGTACC TACAAGACCC GCGCCGAGGT GAAGTTCGAG
  421 GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC
  481 ATCCTGGGGC ACAAGCTGGA GTACAACTTC AACAGCCACA ACGTCTATAT CACGGCCGAC
  541 AAGCAGAAGA ACGGCATCAA GGCTAACTTC AAGATCCGCC ACAACGTTGA GGACGGCAGC
  601 GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG
  661 CCCGACAACC ACTACCTGAG CCATCAGTCC AAGCTGAGCA AAGACCCCAA CGAGAAGCGC
  721 GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TTACACATGG CATGGACGAG
  781 CTGTACAAGT AATCTAGATA ACTGATCATA ATCAGCCATA CCACATTTGT AGAGGTTTTA
  841 CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT GAATGCAATT
  901 GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAGCAAT AGCATCACAA
  961 ATTTCACAAT AAAGCATTTT TTTTCACTGC ATTCTAGTTG TGGTTTTGTC CAAACTCATC
 1021 AATGTATCTT AACGCGTCGA TCATATTCAT AACCTTATAT AACTTCGTAA TAATGTATGC
 1081 TATTACGAGG TTATTAGGTC TGAGAGAGTT AACGTTCCAG CAAGCTTAGG AACCTCGAAC
1141 TCTCGAGACT
//
Primer: PGKpro_F RDB13921_A6B8b_2.seq
>D03070A05_A6B8_1_PGK-proF_A07_01.ab1
    1 ACTACGCGTT CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCATCCATCT AGATCTCGAG
   61 CAGCTGAAGC TTACCGCTAG CATGGATAGA TCCGGAAAGC CTGAACTCAC CGCGACGTCT
  121 GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA GCTCTCGGAG
  181 GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA
  241 AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC
  301 GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT GACCTATTGC
  361 ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT
  421 GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG
  481 AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC
  541 ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC
  601 AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA
  661 GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA CAATGGCCGC
  721 ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA CGAGGTCGCC
  781 AACATCTTCT TCTGGAGCCG TGGTTGGCTT GTATGGAGCA GCAGACGCGC TACTTCGAGC
  841 GGAGGCATCC GGAGCTTGCA GGATCGCCGC GGCTCCGGGC GTATATGCTC CGCATTGGTC
  901 TTGACCAACT CTATCAGAGC TTGGTTGACG CAATTTCGAT GATGCAGCTT GGGCGCAAGG
  961 GTTCGATGCG ACGCATCGTT CCGATCGGAG TCTGGGACTG TCGGGCGTAC ACAAATCGCC
 1021 GCAGAGCGCG CCGTCTTGGA ACCGAATGCT GTGTAGAGTA CCTCGCGATA GTGAAATCGG
 1081 ACGCTCAGCA CCTGTCGAGC AAGGATTGCC TTAGCTGCGG CCCCTCACGA CTCGCTGAGC
1141 TGTC
//
Primer: PGK_N_rev RDB13921_A6B8c_2.seq
>D03070A05_A6B8_1_PGK-N-rev_A06_03.ab1
    1 CATGGGCGCT CCCTACCGGT AGATTTCGAG GTCGAGATCC TAAGCTTGGC TGGACGTAAA
   61 CTCCTCTTCA GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT
  121 ATTGAATATG ATCGACGCGT TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT
  181 GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT
  241 TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA
  301 GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA
  361 TTATGATCAG TTATCTAGAT TACTTGTACA GCTCGTCCAT GCCATGTGTA ATCCCGGCGG
  421 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGCT
  481 TGGACTGATG GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG
  541 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCAACGTTG TGGCGGATCT
  601 TGAAGTTAGC CTTGATGCCG TTCTTCTGCT TGTCGGCCGT GATATAGACG TTGTGGCTGT
  661 TGAAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT
  721 TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT
  781 AGGTACCGTC GTCCTTGAAA GAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG
  841 ACTTGAAGAA GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GCTGAAAGCA GCCACGCCGT
  901 AGCCGAAGGT GGTCACGAGG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA
  961 ACTTCAGGGT CAAGCTTGCC GTTTGGTGGC ATCGCCCTCG CCCTCGCCCG CGGACGCTGA
 1021 ACTTTGTGGC CGTTAACGTC GGCCGTCAAG CTCGACCAGA TGGCACCACC CCGGTGAACA
 1081 GCTCTTCGCC ATGCCTCACA ATGCTAGCGC GCATGCACGG GATCCGAGCT CCATTTCGCC
1141 CCTATTTAGT GAAGATCCGC A
//
Primer: M13R RDB13921_A6B8d_2.seq
>D03070A05_A6B8_1_M13R_B01_04.ab1
    1 CTTCGCGCAT TACCCTCACT AAAGGGAACA AAAGCTGGGT ACCGGATCCT AGTGAACCTC
   61 TTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATATT AAGGGTTCCG
  121 GATCTCGACC AGCTTCTGAT GGAATTAGAA CTTGGCAAAA CAATACTGAG AATGAAGTGT
  181 ATGTGGAACA GAGGCTGCTG ATCTCGTTCT TCAGGCTATG AAACTGACAC ATTTGGAAAC
  241 CACAGTACTT AGAACCACAA AGTGGGAATC AAGAGAAAAA CAATGATCCC ACGAGAGATC
  301 TATAGATCTA TAGATCATGA GTGGGAGGAA TGAGCTGGCC CTTAATTTGG TTTTGCTTGT
  361 TTAAATTATG ATATCCAACT ATGAAACATT ATCATAAAGC AATAGTAAAG AGCCTTCAGT
  421 AAAGAGCAGG CATTTATCTA ATCCCACCCC ACCCCCACCC CCGTAGCTCC AATCCTTCCA
  481 TTCAAAATGT AGGTACTCTG TTCTCACCCT TCTTAACAAA GTATGACAGG AAAAACTTCC
  541 ATTTTAGTGG ACATCTTTAT TGTTTAATAG ATCATCAATT TCTGCATCCC GGGGATCTGA
  601 TATCATCGAT GCATGGGGTC GTGCGCTCCT TTCGGTCGGG CGCTGCGGGT CGTGGGGCGG
  661 GCGGCTAGCC TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC CACTATCGGC
  721 GAGTACTTCT ACACAGCCAT CGGTCCAGAC GGCCGCGCTT CTGCGGGCGA TTTGTGTACG
  781 CCCCGACAGT CCCGGCTCCG GATCGGGACG ATTGCGTCGC ATCGACCCTG CGCCCAAGCT
  841 GCATCATCGA AAATTGCCCG TCAACCAAAG CTCTGATAGA GTTTGGTCAA GACCAATGGC
  901 GGGAGCATAA TACCGCCCGG GGAGCCCGCG GGCGAATCCT GCAAGGCTCC GGGATGCCTC
  961 CGGCTCGAAA GTAAGCGCGT CTGGCTGGCT CCATACAAGG CCAACCACGG GCCTTCCAGA
 1021 AAGAAGATGT TTGGGGACCC TCGTATTGGT GAAAATCTCC CGAACAGTCG GCCCTCCGCC
 1081 TCCAGTTCAA TGAACCGCGC TGTTTAATGC GGGGCCTATT TGTTCCGACT CAGGGGACAT
1141 ATTGTGTT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

2018.09.03

GNP_filter3_RDBDEP_html_180823.pl