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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mAID-BRD4 donor

Donor plasmid to generate BRD4-mAID degron cell lines, hygromycin resistance.

Catalog number RDB18358
Resource name mAID-BRD4 donor
Clone info. Donor plasmid to generate BRD4-mAID degron cell lines, hygromycin resistance, see Fig. 5 of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone pBluescriptII KS- (plasmid)
Selectable markers Ampicillin (E. coli), Hygromycin (mammalian cell).
Gene/insert name human BRD4 genomic Arabidopsis thaliana IAA17 cDNA
Depositor|Developer Kanemaki, Masato |
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External Database
human BRD4 Arabidopsis thaliana IAA17

        Reference sequence
          
         
        Sequence (full) RDB18358zzk01.seq provided by the depositor

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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        RDB18358 mAID-BRD4 donor DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Original reference

        original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Auxin Inducible Degron (AID) System (English text)
        Featured content Auxin Inducible Degron (AID) System (Japanese text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB18358_B2Hap1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: pUC-M13_-100
        Region: T7 pro,insert 5'(homology arm.1,HygR)
        Sequence file: RDB18358_B2Haa.seq check
        >D07105C8_B2Ha_3_pUC-M13_-100_A02_02_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN TGGNNNNNNC NNGGGTTTTC CCNGTCNCGA CGTTGTAAAA
           61 CGACGGCCAG TGNNCNCNCN NAATACGACT CACTATAGGG CGAATTGGAG CTCCAAATGA
          121 AATGATTGAC TTTGCTCGAT GCTTTGTGTG TGATAAATGC AAGGAGTTTT AATGTAAATG
          181 TCATATGTGG GAGCCTGCAC ATCATCCCCT TCCACACACA GGCAGCATCT CCTAAAAACT
          241 CACAGGTGGG AAAAGGGATG TGCAGGTTTG TGAGACGTGG CTTCATTGTA CATCCTTCCC
          301 CATGACCTTC ATAGTTCCCA GAGCCTTTGC TGGCTCCTAG TGGGCTCTCA CCACCTCACT
          361 CTGCCTCCTC TGTTGGTTTG TGGTCTCCTC CCTGCTGGAG GGTAAGTCTT TGGGGAGAGG
          421 AGACATTGGA ACCCTTTCAG TCAAAGCATG GGGTGGTCCC TCTGGCCAAC TTGGCTAGCT
          481 ACCGCCAGGG AACTCAGTCC TTGGAGACCA CAGCCAGAGG GACTTGTCAT TCTGAGCGGT
          541 GCCCATCTGC TGACTGATAT CTCACGGGGG CTCTTCTCTT CCTTTGTAGA GTGCCTGGTG
          601 AAGAATGTGA TGGGATCACT AGCAAGCTTG ATATCGCCAC CATGAAAAAG CCTGAACTCA
          661 CCGCGACGTC TGTCGAGAAG TTTCTGATCG AAAAGTTCGA CAGCGTCTCC GACCTGATGC
          721 AGCTCTCGGA NGGCGAAGAN TCTCGTGCTT TCAGCTTCGA TGTANGAGGG CGTGGANATG
          781 TCCTGCGGGT AAATAGCTGC GCCGATGGTT TCTACNAAGA TCGTTATGTT TATCGGCACT
          841 TTGCATCGGC CGCGCTCCCG ATTCCNGAAG TGCTTGACNT TGGGGAATTC AGCGANAGCC
          901 TGNNCTATTG CATCTNCNGC CNNGCACANG GNGTCACGTN NCNNGANCTG CCTGAAANCN
          961 AACTGCCCGC TNTNCTNCAN CCGGTCGCNG NNNNNNANNG NATGCNNNNG CNNNNNNNNN
        1021 N
        //
        Primer: pUC-CAPbs_up_F
        Region: T3 pro,insert 3'(homology arm.2,Linker,mAID)
        Sequence file: RDB18358_B2Hab.seq check
        >D07105C8_B2Ha_3_pUC-CAPbs_up_F_F02_06_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCNC ANCGCAATTA ATGTGAGTTA GCTCACTCAT
           61 TAGGCACCCC AGGCTTTACA CTTTATGCTC CCGGCTCGTA TGTTGTGTGG AATTGTGAGC
          121 GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCG CGCAATTAAC
          181 CCTCACTAAA GGGAACAAAA GCTGGGTACC TCAACTGATC AGAACTGCTG AAACCACTCA
          241 CTCAAGGGCC CTGCAGCTCT CACCATGATT CCAAATGCAT CTCCTACCCT CCCCCCAGGA
          301 ACTGCCCTGA CCAGGAGACA TGCAGGAGAC GACCTCCAGG ACGGCCACCC TGGGCCCTGC
          361 CCACACTACT CACAGGGAGG TTCAGCTTGA CGGCATCCAC AGGCTGCTGG AAAGGCCATG
          421 CAAACTGGTG TTTCCATAGT GTCTTGAGCA CCACTCTGAG CAGGTATTGC AGTTGGTTGG
          481 TCTGCCTCTT GGGCTTGTTA GGGTTGGAGG TCTCTGGGGG CGGGGGGTTG GTGCTGGCTG
          541 CGTTGGCTGG CTGGGGTTGG GCCTGGGCCT GTGTTGTAGA CATTTGGGAA GTTTCTAGTC
          601 CATCCCCCAT TACTGGCAGA TTTCTCAATC TCGTCCCAGG ACCACTCTCC GCAGACATGG
          661 ATCCGGCGCC TGCACCAGCG CCTTTATACA TCCTCAAATC GATTTTCCTC AAGTACGGTG
          721 CTCCGTCCAT TGATACCTTC ACGAACGCCG CCGCCTCCGG GCCACCGCTT GATTTTTGGC
          781 ANGAAACCAT CACGTTCTTC CGGTATGATC TCACCGGTGG CCATCCCNCA ACTTGTGCCT
          841 TGNCCGGANG TTTGGCTGGA TCTTNNNGAC AAGCACTCNT CTCCTTAGAT CTNNNNCCAN
          901 GGNTCTCCTN CNNCGTCTCC NGCCTGCTTC AGCNNNTGAA NTTAGTANCT NCNCNTCCTG
          961 ANTNCNTTTG NCNTCGNANN ANTGNTGGGG NNNNGGTTTN NNNNNTCGGC NANNNNTTCN
        1021 ACNCAGNCNT CNGNNCCANA NNNNNNCNCN TNNGNNNNNN NNNN
        //
        Primer: HygR-M_R
        Region: insert mid(HygR,homology arm.1)
        Sequence file: RDB18358_B2Hac.seq check
        >D07105C8_B2Ha_3_HygR-M_R_C02_08_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NTGNNCGATT CCTTGCGGTC CGAATGGGCC GAACCCGCTC
           61 GTCTGGCTNN NNNNNNNCGC AGCGATCGCA TCCATGGCCT CCGCGACCGG CTGNNNAACA
          121 GCGGGCAGTT CGGTTTCAGG CAGGTCTTGC AACGTGACAC CCTGTGCACG GCGGGAGATG
          181 CAATAGGTCA GGCTCTCGCT GAATTCCCCA ATGTCAAGCA CTTCCGGAAT CGGGAGCGCG
          241 GCCGATGCAA AGTGCCGATA AACATAACGA TCTTTGTAGA AACCATCGGC GCAGCTATTT
          301 ACCCGCAGGA CATATCCACG CCCTCCTACA TCGAAGCTGA AAGCACGAGA TTCTTCGCCC
          361 TCCGAGAGCT GCATCAGGTC GGAGACGCTG TCGAACTTTT CGATCAGAAA CTTCTCGACA
          421 GACGTCGCGG TGAGTTCAGG CTTTTTCATG GTGGCGATAT CAAGCTTGCT AGTGATCCCA
          481 TCACATTCTT CACCAGGCAC TCTACAAAGG AAGAGAAGAG CCCCCGTGAG ATATCAGTCA
          541 GCAGATGGGC ACCGCTCAGA ATGACAAGTC CCTCTGGCTG TGGTCTCCAA GGACTGAGTT
          601 CCCTGGCGGT AGCTAGCCAA GTTGGCCAGA GGGACCACCC CATGCTTTGA CTGAAAGGGT
          661 TCCAATGTCT CCTCTCCCCA AAGACTTACC CTCCAGCAGG GAGGAGACCA CAAACCAACA
          721 GAGGANGCAG AGTGAGGTGG TGAGAGCCCA CTANGAGCCA GCAAAGGCTC TGGGAACTAT
          781 GAAGGTCATG GGGAANGATG TACAATGAAN CCACGTCTCA CAAACCTGCA CATCCCTTTT
          841 NCCACCNGTG ANTTTTTAGG ANANGCTGCC NGTGTGTGGA ANGGGATGAN GTGCANGCTC
          901 CCACATATGN NATTTACATN AAAACTCCTT GCATTTATCA CACNCAAAGC ATCGNNCAAA
        961 GTCNNTCATT TTCNNTTGGA NCTCCNNNNN GCCCNNNNNN GAGNNNNNNN NNN
        //
        Primer: mSLGE_R2
        Region: insert mid(Linker,mAID,P2A,HygR)
        Sequence file: RDB18358_B2Had.seq check
        >D07105C8_B2Ha_3_mSLGE_R2_D01_04_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNCNN NNNNNNNTAC TGGCAGNNTT CTCNATCTCG TCCCAGGACC
           61 ACTCTCCGCA NACNNNNNNN NGGCGCCTGC ACCAGCGCCT TTATACATCC TCAAATCGAT
          121 TTTCCTCAAG TACGGTGCTC CGTCCATTGA TACCTTCACG AACGCCGCCG CCTCCGGGCC
          181 ACCGCTTGAT TTTTGGCAGG AAACCATCAC GTTCTTCCGG TATGATCTCA CCGGTGGCCA
          241 TCCCACAACT TGTGCCTTGG CCGGAGGTTT GGCTGGATCT TTAGGACAAG CACTCTTCTC
          301 CTTAGATCTA GGTCCAGGGT TCTCCTCCAC GTCTCCAGCC TGCTTCAGCA GGCTGAAGTT
          361 AGTAGCTCCG CTTCCTGATT CCTTTGCCCT CGGACGAGTG CTGGGGCGTC GGTTTCCACT
          421 ATCGGCGAGT ACTTCTACAC AGCCATCGGT CCAGACGGCC GCGCTTCTGC GGGCGATTTG
          481 TGTACGCCCG ACAGTCCCGG CTCCGGATCG GACGATTGCG TCGCATCGAC CCTGCGCCCA
          541 AGCTGCATCA TCGAAATTGC CGTCAACCAA GCTCTGATAG AGTTGGTCAA GACCAATGCG
          601 GAGCATATAC GCCCGGAGCC GCGGCGATCC TGCAAGCTCC GGATGCCTCC GCTCGAAGTA
          661 GCGCGTCTGC TGCTCCATAC AAGCCAACCA CGGCCTCCAG AAGAAGATGT TGGCGACCTC
          721 GTATTGGGAA TCCCCGAACA TCGCCTCGCT CCAGTCAATG ACCGCTGTTA TGCGGCCATT
          781 GTCCGTCAGG ACATTGTTGG AGCCGAAATC CGCGTGCNCG ANGTGNCGGA NTTCGGGGCA
          841 GTCCTCGGCC CAAAGCATCA GCTCATCGAN AGCCTGCGCG ACGGANGCNC TGACGGTGTC
          901 NTCCATCACA GTTTGNCAGT GATACNCANG GGGNTCANCN NTCGCNCATA TGAAATCNCG
          961 CCNNGNANTG NANNNNCCGA NTCCTTGCNG NCCNAATGGG NNNAANCCNC NCNNCNNGNN
        1021 NANNNNGGNN NCNNCNATNN NNNNNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

        2022.09.29

        GNP_filter3_RDBDEP_html_220812.pl