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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mAID-BRD4 donor (#RDB18358)

Donor plasmid to generate BRD4-mAID degron cell lines, hygromycin resistance.

Clone info. Donor plasmid to generate BRD4-mAID degron cell lines, hygromycin resistance, see Fig. 5 of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone pBluescriptII KS- (plasmid)
Selectable markers Ampicillin (E. coli), Hygromycin (mammalian cell).
Gene/insert name human BRD4 genomic Arabidopsis thaliana IAA17 cDNA
Depositor|Developer Kanemaki, Masato |
 
Sequence (full) RDB18358zzk01.seq provided by the depositor

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。

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RDB18358 mAID-BRD4 donor DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The mAID-BRD4 donor was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18358).

Reference section:

Yesbolatova, A., Saito, Y., Kitamoto, N., Makino-Itou, H., Ajima, R., Nakano, R., Nakaoka, H., Fukui, K., Gamo, K., Tominari, Y., Takeuchi, H., Saga, Y., Hayashi, K., Kanemaki, M.T., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18358_B2Hap1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310)
Region: T7 pro,insert 5'(homology arm.1,HygR)
Sequence file: RDB18358_B2Haa.seq check
>D07105C8_B2Ha_3_pUC-M13_-100_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TGGNNNNNNC NNGGGTTTTC CCNGTCNCGA CGTTGTAAAA
   61 CGACGGCCAG TGNNCNCNCN NAATACGACT CACTATAGGG CGAATTGGAG CTCCAAATGA
  121 AATGATTGAC TTTGCTCGAT GCTTTGTGTG TGATAAATGC AAGGAGTTTT AATGTAAATG
  181 TCATATGTGG GAGCCTGCAC ATCATCCCCT TCCACACACA GGCAGCATCT CCTAAAAACT
  241 CACAGGTGGG AAAAGGGATG TGCAGGTTTG TGAGACGTGG CTTCATTGTA CATCCTTCCC
  301 CATGACCTTC ATAGTTCCCA GAGCCTTTGC TGGCTCCTAG TGGGCTCTCA CCACCTCACT
  361 CTGCCTCCTC TGTTGGTTTG TGGTCTCCTC CCTGCTGGAG GGTAAGTCTT TGGGGAGAGG
  421 AGACATTGGA ACCCTTTCAG TCAAAGCATG GGGTGGTCCC TCTGGCCAAC TTGGCTAGCT
  481 ACCGCCAGGG AACTCAGTCC TTGGAGACCA CAGCCAGAGG GACTTGTCAT TCTGAGCGGT
  541 GCCCATCTGC TGACTGATAT CTCACGGGGG CTCTTCTCTT CCTTTGTAGA GTGCCTGGTG
  601 AAGAATGTGA TGGGATCACT AGCAAGCTTG ATATCGCCAC CATGAAAAAG CCTGAACTCA
  661 CCGCGACGTC TGTCGAGAAG TTTCTGATCG AAAAGTTCGA CAGCGTCTCC GACCTGATGC
  721 AGCTCTCGGA NGGCGAAGAN TCTCGTGCTT TCAGCTTCGA TGTANGAGGG CGTGGANATG
  781 TCCTGCGGGT AAATAGCTGC GCCGATGGTT TCTACNAAGA TCGTTATGTT TATCGGCACT
  841 TTGCATCGGC CGCGCTCCCG ATTCCNGAAG TGCTTGACNT TGGGGAATTC AGCGANAGCC
  901 TGNNCTATTG CATCTNCNGC CNNGCACANG GNGTCACGTN NCNNGANCTG CCTGAAANCN
  961 AACTGCCCGC TNTNCTNCAN CCGGTCGCNG NNNNNNANNG NATGCNNNNG CNNNNNNNNN
1021 N
//
Primer: pUC-CAPbs_up_F (Pr0536)
Region: T3 pro,insert 3'(homology arm.2,Linker,mAID)
Sequence file: RDB18358_B2Hab.seq check
>D07105C8_B2Ha_3_pUC-CAPbs_up_F_F02_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCNC ANCGCAATTA ATGTGAGTTA GCTCACTCAT
   61 TAGGCACCCC AGGCTTTACA CTTTATGCTC CCGGCTCGTA TGTTGTGTGG AATTGTGAGC
  121 GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCG CGCAATTAAC
  181 CCTCACTAAA GGGAACAAAA GCTGGGTACC TCAACTGATC AGAACTGCTG AAACCACTCA
  241 CTCAAGGGCC CTGCAGCTCT CACCATGATT CCAAATGCAT CTCCTACCCT CCCCCCAGGA
  301 ACTGCCCTGA CCAGGAGACA TGCAGGAGAC GACCTCCAGG ACGGCCACCC TGGGCCCTGC
  361 CCACACTACT CACAGGGAGG TTCAGCTTGA CGGCATCCAC AGGCTGCTGG AAAGGCCATG
  421 CAAACTGGTG TTTCCATAGT GTCTTGAGCA CCACTCTGAG CAGGTATTGC AGTTGGTTGG
  481 TCTGCCTCTT GGGCTTGTTA GGGTTGGAGG TCTCTGGGGG CGGGGGGTTG GTGCTGGCTG
  541 CGTTGGCTGG CTGGGGTTGG GCCTGGGCCT GTGTTGTAGA CATTTGGGAA GTTTCTAGTC
  601 CATCCCCCAT TACTGGCAGA TTTCTCAATC TCGTCCCAGG ACCACTCTCC GCAGACATGG
  661 ATCCGGCGCC TGCACCAGCG CCTTTATACA TCCTCAAATC GATTTTCCTC AAGTACGGTG
  721 CTCCGTCCAT TGATACCTTC ACGAACGCCG CCGCCTCCGG GCCACCGCTT GATTTTTGGC
  781 ANGAAACCAT CACGTTCTTC CGGTATGATC TCACCGGTGG CCATCCCNCA ACTTGTGCCT
  841 TGNCCGGANG TTTGGCTGGA TCTTNNNGAC AAGCACTCNT CTCCTTAGAT CTNNNNCCAN
  901 GGNTCTCCTN CNNCGTCTCC NGCCTGCTTC AGCNNNTGAA NTTAGTANCT NCNCNTCCTG
  961 ANTNCNTTTG NCNTCGNANN ANTGNTGGGG NNNNGGTTTN NNNNNTCGGC NANNNNTTCN
1021 ACNCAGNCNT CNGNNCCANA NNNNNNCNCN TNNGNNNNNN NNNN
//
Primer: HygR-M_R (Pr0769)
Region: insert mid(HygR,homology arm.1)
Sequence file: RDB18358_B2Hac.seq check
>D07105C8_B2Ha_3_HygR-M_R_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTGNNCGATT CCTTGCGGTC CGAATGGGCC GAACCCGCTC
   61 GTCTGGCTNN NNNNNNNCGC AGCGATCGCA TCCATGGCCT CCGCGACCGG CTGNNNAACA
  121 GCGGGCAGTT CGGTTTCAGG CAGGTCTTGC AACGTGACAC CCTGTGCACG GCGGGAGATG
  181 CAATAGGTCA GGCTCTCGCT GAATTCCCCA ATGTCAAGCA CTTCCGGAAT CGGGAGCGCG
  241 GCCGATGCAA AGTGCCGATA AACATAACGA TCTTTGTAGA AACCATCGGC GCAGCTATTT
  301 ACCCGCAGGA CATATCCACG CCCTCCTACA TCGAAGCTGA AAGCACGAGA TTCTTCGCCC
  361 TCCGAGAGCT GCATCAGGTC GGAGACGCTG TCGAACTTTT CGATCAGAAA CTTCTCGACA
  421 GACGTCGCGG TGAGTTCAGG CTTTTTCATG GTGGCGATAT CAAGCTTGCT AGTGATCCCA
  481 TCACATTCTT CACCAGGCAC TCTACAAAGG AAGAGAAGAG CCCCCGTGAG ATATCAGTCA
  541 GCAGATGGGC ACCGCTCAGA ATGACAAGTC CCTCTGGCTG TGGTCTCCAA GGACTGAGTT
  601 CCCTGGCGGT AGCTAGCCAA GTTGGCCAGA GGGACCACCC CATGCTTTGA CTGAAAGGGT
  661 TCCAATGTCT CCTCTCCCCA AAGACTTACC CTCCAGCAGG GAGGAGACCA CAAACCAACA
  721 GAGGANGCAG AGTGAGGTGG TGAGAGCCCA CTANGAGCCA GCAAAGGCTC TGGGAACTAT
  781 GAAGGTCATG GGGAANGATG TACAATGAAN CCACGTCTCA CAAACCTGCA CATCCCTTTT
  841 NCCACCNGTG ANTTTTTAGG ANANGCTGCC NGTGTGTGGA ANGGGATGAN GTGCANGCTC
  901 CCACATATGN NATTTACATN AAAACTCCTT GCATTTATCA CACNCAAAGC ATCGNNCAAA
961 GTCNNTCATT TTCNNTTGGA NCTCCNNNNN GCCCNNNNNN GAGNNNNNNN NNN
//
Primer: mSLGE_R2 (Pr0816)
Region: insert mid(Linker,mAID,P2A,HygR)
Sequence file: RDB18358_B2Had.seq check
>D07105C8_B2Ha_3_mSLGE_R2_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNCNN NNNNNNNTAC TGGCAGNNTT CTCNATCTCG TCCCAGGACC
   61 ACTCTCCGCA NACNNNNNNN NGGCGCCTGC ACCAGCGCCT TTATACATCC TCAAATCGAT
  121 TTTCCTCAAG TACGGTGCTC CGTCCATTGA TACCTTCACG AACGCCGCCG CCTCCGGGCC
  181 ACCGCTTGAT TTTTGGCAGG AAACCATCAC GTTCTTCCGG TATGATCTCA CCGGTGGCCA
  241 TCCCACAACT TGTGCCTTGG CCGGAGGTTT GGCTGGATCT TTAGGACAAG CACTCTTCTC
  301 CTTAGATCTA GGTCCAGGGT TCTCCTCCAC GTCTCCAGCC TGCTTCAGCA GGCTGAAGTT
  361 AGTAGCTCCG CTTCCTGATT CCTTTGCCCT CGGACGAGTG CTGGGGCGTC GGTTTCCACT
  421 ATCGGCGAGT ACTTCTACAC AGCCATCGGT CCAGACGGCC GCGCTTCTGC GGGCGATTTG
  481 TGTACGCCCG ACAGTCCCGG CTCCGGATCG GACGATTGCG TCGCATCGAC CCTGCGCCCA
  541 AGCTGCATCA TCGAAATTGC CGTCAACCAA GCTCTGATAG AGTTGGTCAA GACCAATGCG
  601 GAGCATATAC GCCCGGAGCC GCGGCGATCC TGCAAGCTCC GGATGCCTCC GCTCGAAGTA
  661 GCGCGTCTGC TGCTCCATAC AAGCCAACCA CGGCCTCCAG AAGAAGATGT TGGCGACCTC
  721 GTATTGGGAA TCCCCGAACA TCGCCTCGCT CCAGTCAATG ACCGCTGTTA TGCGGCCATT
  781 GTCCGTCAGG ACATTGTTGG AGCCGAAATC CGCGTGCNCG ANGTGNCGGA NTTCGGGGCA
  841 GTCCTCGGCC CAAAGCATCA GCTCATCGAN AGCCTGCGCG ACGGANGCNC TGACGGTGTC
  901 NTCCATCACA GTTTGNCAGT GATACNCANG GGGNTCANCN NTCGCNCATA TGAAATCNCG
  961 CCNNGNANTG NANNNNCCGA NTCCTTGCNG NCCNAATGGG NNNAANCCNC NCNNCNNGNN
1021 NANNNNGGNN NCNNCNATNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]