Please review the QC test results indicated by icon below as well as clone information before placing your order.

mAID-BRD4 donor

Donor plasmid to generate BRD4-mAID degron cell lines, hygromycin resistance.

Sequence (full)	RDB18358zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18358_B2Hap1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310) Region: T7 pro,insert 5'(homology arm.1,HygR) Sequence file: RDB18358_B2Haa.seq
>D07105C8_B2Ha_3_pUC-M13_-100_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN TGGNNNNNNC NNGGGTTTTC CCNGTCNCGA CGTTGTAAAA    61 CGACGGCCAG TGNNCNCNCN NAATACGACT CACTATAGGG CGAATTGGAG CTCCAAATGA   121 AATGATTGAC TTTGCTCGAT GCTTTGTGTG TGATAAATGC AAGGAGTTTT AATGTAAATG   181 TCATATGTGG GAGCCTGCAC ATCATCCCCT TCCACACACA GGCAGCATCT CCTAAAAACT   241 CACAGGTGGG AAAAGGGATG TGCAGGTTTG TGAGACGTGG CTTCATTGTA CATCCTTCCC   301 CATGACCTTC ATAGTTCCCA GAGCCTTTGC TGGCTCCTAG TGGGCTCTCA CCACCTCACT   361 CTGCCTCCTC TGTTGGTTTG TGGTCTCCTC CCTGCTGGAG GGTAAGTCTT TGGGGAGAGG   421 AGACATTGGA ACCCTTTCAG TCAAAGCATG GGGTGGTCCC TCTGGCCAAC TTGGCTAGCT   481 ACCGCCAGGG AACTCAGTCC TTGGAGACCA CAGCCAGAGG GACTTGTCAT TCTGAGCGGT   541 GCCCATCTGC TGACTGATAT CTCACGGGGG CTCTTCTCTT CCTTTGTAGA GTGCCTGGTG   601 AAGAATGTGA TGGGATCACT AGCAAGCTTG ATATCGCCAC CATGAAAAAG CCTGAACTCA   661 CCGCGACGTC TGTCGAGAAG TTTCTGATCG AAAAGTTCGA CAGCGTCTCC GACCTGATGC   721 AGCTCTCGGA NGGCGAAGAN TCTCGTGCTT TCAGCTTCGA TGTANGAGGG CGTGGANATG   781 TCCTGCGGGT AAATAGCTGC GCCGATGGTT TCTACNAAGA TCGTTATGTT TATCGGCACT   841 TTGCATCGGC CGCGCTCCCG ATTCCNGAAG TGCTTGACNT TGGGGAATTC AGCGANAGCC   901 TGNNCTATTG CATCTNCNGC CNNGCACANG GNGTCACGTN NCNNGANCTG CCTGAAANCN   961 AACTGCCCGC TNTNCTNCAN CCGGTCGCNG NNNNNNANNG NATGCNNNNG CNNNNNNNNN 1021 N //
Primer: pUC-CAPbs_up_F (Pr0536) Region: T3 pro,insert 3'(homology arm.2,Linker,mAID) Sequence file: RDB18358_B2Hab.seq
>D07105C8_B2Ha_3_pUC-CAPbs_up_F_F02_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCNC ANCGCAATTA ATGTGAGTTA GCTCACTCAT    61 TAGGCACCCC AGGCTTTACA CTTTATGCTC CCGGCTCGTA TGTTGTGTGG AATTGTGAGC   121 GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCG CGCAATTAAC   181 CCTCACTAAA GGGAACAAAA GCTGGGTACC TCAACTGATC AGAACTGCTG AAACCACTCA   241 CTCAAGGGCC CTGCAGCTCT CACCATGATT CCAAATGCAT CTCCTACCCT CCCCCCAGGA   301 ACTGCCCTGA CCAGGAGACA TGCAGGAGAC GACCTCCAGG ACGGCCACCC TGGGCCCTGC   361 CCACACTACT CACAGGGAGG TTCAGCTTGA CGGCATCCAC AGGCTGCTGG AAAGGCCATG   421 CAAACTGGTG TTTCCATAGT GTCTTGAGCA CCACTCTGAG CAGGTATTGC AGTTGGTTGG   481 TCTGCCTCTT GGGCTTGTTA GGGTTGGAGG TCTCTGGGGG CGGGGGGTTG GTGCTGGCTG   541 CGTTGGCTGG CTGGGGTTGG GCCTGGGCCT GTGTTGTAGA CATTTGGGAA GTTTCTAGTC   601 CATCCCCCAT TACTGGCAGA TTTCTCAATC TCGTCCCAGG ACCACTCTCC GCAGACATGG   661 ATCCGGCGCC TGCACCAGCG CCTTTATACA TCCTCAAATC GATTTTCCTC AAGTACGGTG   721 CTCCGTCCAT TGATACCTTC ACGAACGCCG CCGCCTCCGG GCCACCGCTT GATTTTTGGC   781 ANGAAACCAT CACGTTCTTC CGGTATGATC TCACCGGTGG CCATCCCNCA ACTTGTGCCT   841 TGNCCGGANG TTTGGCTGGA TCTTNNNGAC AAGCACTCNT CTCCTTAGAT CTNNNNCCAN   901 GGNTCTCCTN CNNCGTCTCC NGCCTGCTTC AGCNNNTGAA NTTAGTANCT NCNCNTCCTG   961 ANTNCNTTTG NCNTCGNANN ANTGNTGGGG NNNNGGTTTN NNNNNTCGGC NANNNNTTCN 1021 ACNCAGNCNT CNGNNCCANA NNNNNNCNCN TNNGNNNNNN NNNN //
Primer: HygR-M_R (Pr0769) Region: insert mid(HygR,homology arm.1) Sequence file: RDB18358_B2Hac.seq
>D07105C8_B2Ha_3_HygR-M_R_C02_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NTGNNCGATT CCTTGCGGTC CGAATGGGCC GAACCCGCTC    61 GTCTGGCTNN NNNNNNNCGC AGCGATCGCA TCCATGGCCT CCGCGACCGG CTGNNNAACA   121 GCGGGCAGTT CGGTTTCAGG CAGGTCTTGC AACGTGACAC CCTGTGCACG GCGGGAGATG   181 CAATAGGTCA GGCTCTCGCT GAATTCCCCA ATGTCAAGCA CTTCCGGAAT CGGGAGCGCG   241 GCCGATGCAA AGTGCCGATA AACATAACGA TCTTTGTAGA AACCATCGGC GCAGCTATTT   301 ACCCGCAGGA CATATCCACG CCCTCCTACA TCGAAGCTGA AAGCACGAGA TTCTTCGCCC   361 TCCGAGAGCT GCATCAGGTC GGAGACGCTG TCGAACTTTT CGATCAGAAA CTTCTCGACA   421 GACGTCGCGG TGAGTTCAGG CTTTTTCATG GTGGCGATAT CAAGCTTGCT AGTGATCCCA   481 TCACATTCTT CACCAGGCAC TCTACAAAGG AAGAGAAGAG CCCCCGTGAG ATATCAGTCA   541 GCAGATGGGC ACCGCTCAGA ATGACAAGTC CCTCTGGCTG TGGTCTCCAA GGACTGAGTT   601 CCCTGGCGGT AGCTAGCCAA GTTGGCCAGA GGGACCACCC CATGCTTTGA CTGAAAGGGT   661 TCCAATGTCT CCTCTCCCCA AAGACTTACC CTCCAGCAGG GAGGAGACCA CAAACCAACA   721 GAGGANGCAG AGTGAGGTGG TGAGAGCCCA CTANGAGCCA GCAAAGGCTC TGGGAACTAT   781 GAAGGTCATG GGGAANGATG TACAATGAAN CCACGTCTCA CAAACCTGCA CATCCCTTTT   841 NCCACCNGTG ANTTTTTAGG ANANGCTGCC NGTGTGTGGA ANGGGATGAN GTGCANGCTC   901 CCACATATGN NATTTACATN AAAACTCCTT GCATTTATCA CACNCAAAGC ATCGNNCAAA 961 GTCNNTCATT TTCNNTTGGA NCTCCNNNNN GCCCNNNNNN GAGNNNNNNN NNN //
Primer: mSLGE_R2 (Pr0816) Region: insert mid(Linker,mAID,P2A,HygR) Sequence file: RDB18358_B2Had.seq
>D07105C8_B2Ha_3_mSLGE_R2_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNCNN NNNNNNNTAC TGGCAGNNTT CTCNATCTCG TCCCAGGACC    61 ACTCTCCGCA NACNNNNNNN NGGCGCCTGC ACCAGCGCCT TTATACATCC TCAAATCGAT   121 TTTCCTCAAG TACGGTGCTC CGTCCATTGA TACCTTCACG AACGCCGCCG CCTCCGGGCC   181 ACCGCTTGAT TTTTGGCAGG AAACCATCAC GTTCTTCCGG TATGATCTCA CCGGTGGCCA   241 TCCCACAACT TGTGCCTTGG CCGGAGGTTT GGCTGGATCT TTAGGACAAG CACTCTTCTC   301 CTTAGATCTA GGTCCAGGGT TCTCCTCCAC GTCTCCAGCC TGCTTCAGCA GGCTGAAGTT   361 AGTAGCTCCG CTTCCTGATT CCTTTGCCCT CGGACGAGTG CTGGGGCGTC GGTTTCCACT   421 ATCGGCGAGT ACTTCTACAC AGCCATCGGT CCAGACGGCC GCGCTTCTGC GGGCGATTTG   481 TGTACGCCCG ACAGTCCCGG CTCCGGATCG GACGATTGCG TCGCATCGAC CCTGCGCCCA   541 AGCTGCATCA TCGAAATTGC CGTCAACCAA GCTCTGATAG AGTTGGTCAA GACCAATGCG   601 GAGCATATAC GCCCGGAGCC GCGGCGATCC TGCAAGCTCC GGATGCCTCC GCTCGAAGTA   661 GCGCGTCTGC TGCTCCATAC AAGCCAACCA CGGCCTCCAG AAGAAGATGT TGGCGACCTC   721 GTATTGGGAA TCCCCGAACA TCGCCTCGCT CCAGTCAATG ACCGCTGTTA TGCGGCCATT   781 GTCCGTCAGG ACATTGTTGG AGCCGAAATC CGCGTGCNCG ANGTGNCGGA NTTCGGGGCA   841 GTCCTCGGCC CAAAGCATCA GCTCATCGAN AGCCTGCGCG ACGGANGCNC TGACGGTGTC   901 NTCCATCACA GTTTGNCAGT GATACNCANG GGGNTCANCN NTCGCNCATA TGAAATCNCG   961 CCNNGNANTG NANNNNCCGA NTCCTTGCNG NCCNAATGGG NNNAANCCNC NCNNCNNGNN 1021 NANNNNGGNN NCNNCNATNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl