Sequencing and PCR primers for primer information.
A plasmid for construction of C-terminal Stag-3FLAG tagging donors.
| Catalog number | RDB13928 |
|---|---|
| Resource name | pMK285 (Stag-3FLAG-Bsr) |
| Alternative name | Stag-3FLAG-Bsr |
| Clone info. | A plasmid for construction of C-terminal Stag-3FLAG tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone. |
| Vector backbone | pBluescriptII KS- (Plasmid) |
| Size of vector backbone | 3.0 kb |
| Selectable markers | Am^r |
| Depositor | Kanemaki, Masato | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB13928 | pMK285 (Stag-3FLAG-Bsr) | DNA solution |
Electronic file
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
|---|---|
| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
| Featured content | Genome Editing (English text) |
| Featured content | Genome Editing (Japanese text) |
| Featured content | Empty Backbone (English text) |
| Featured content | Empty Backbone (Japanese text) |
References
| original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
|---|
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13928_A6B8p1_v2.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
| Primer: M13_-40 | RDB13928_A6B8a_2.seq |
|---|---|
|
>D03070A12_A6B8_1_M13(-40)_E03_15.ab1 1 AAGCGGACGC GCGTATACGA CTCACTATAG GGCGAATTGG AGCTCGGATC CGGTGCAGGC 61 GCCAAGGAAA CCGCCGCTGC AAAATTCGAA CGCCAGCACA TGGACAGCCG ATCCCGGGCT 121 GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT 181 GACAAGTAGT GTACAAGTAA TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG 241 AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA 301 ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 361 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 421 AACTCATCAA TGTATCTTAA CGCGTCGATC ATATTCAATA ACCCTTAATA TAACTTCGTA 481 TAATGTATGC TATACGAAGT TATTAGGTCT GAAGAGGAGT TTACGTCCAG CCAAGCTTAG 541 GATCTCGACC TCGAAATTCT ACCGGGTAGG GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC 601 ATGCGCTTTA GCAGCCCCGC TGGGCACTTG GCGCTACACA AGTGGCCTCT GGCCTCGCAC 661 ACATTCCACA TCCACCGGTA GGCGCCAACC GACTCCGTTC TTTGGTGGCC CCTTCGCGCC 721 ACCTTCTACT CCTCCCCTAG TCAGGAAGTT CCCCCCCGCC CCGCAGCTCG CGTCGTGCAG 781 GACGTGACAA ATGGAAGTAG CACGTCTCAC TAGTCTCGTG CAGATGGACA GCACCGCTGA 841 GCAATGGAAG CGGGTAGGCC TTTGGGGCAG CGGCCAATAG CAGCTTTGCT CCTTCGCTTT 901 CTGGGCTCAG AGGCTGGGAA GGGGTGGGTC CGGGGGCGGG CTCAGGGGCG GGCTCAGGGC 961 GGGGCGGGCG CCCGAAGTCC TCCCGAGGCC CGGCATTCTG CACGCTTCAA AAGCGCACGT 1021 CTGCCGCGCT GTTCTCCTCT TCCTCATCTC CGGGCCTTTC GACTGCATCC ATCTAGATCT 1081 CGAGCAGCTG AAGCTACGCT AGCATGAAAA CATTACCATT TCTCACCAGA TCTAGATAAG 1141 TAGGAGTAAG CGGACAGAGA CAAGAATTAC A // |
|
| Primer: PGKpro_F | RDB13928_A6B8b_2.seq |
|
>D03070A12_A6B8_1_PGK-proF_F07_16.ab1 1 AGGGGCGTTC TCTCTTCTCA TCTCCGGGCC TTTCGACCTG CATCCATCTA GATCTCGAGC 61 AGCTGAAGCT TACCGCTAGC ATGAAAACAT TTAACATTTC TCAACAAGAT CTAGAATTAG 121 TAGAAGTAGC GACAGAGAAG ATTACAATGC TTTATGAGGA TAATAAACAT CATGTGGGAG 181 CGGCAATTCG TACGAAAACA GGAGAAATCA TTTCGGCAGT ACATATTGAA GCGTATATAG 241 GACGAGTAAC TGTTTGTGCA GAAGCCATTG CGATTGGTAG TGCAGTTTCG AATGGACAAA 301 AGGATTTTGA CACGATTGTA GCTGTTAGAC ACCCTTATTC TGACGAAGTA GATAGAAGTA 361 TTCGAGTGGT AAGTCCTTGT GGTATGTGTA GGGAGTTGAT TTCAGACTAT GCACCAGATT 421 GTTTTGTGTT AATAGAAATG AATGGCAAGT TAGTCAAAAC TACGATTGAA GAACTCATTC 481 CACTCAAATA TACCCGAAAT TAAGCTAGCC GCCCGCCCCA CGACCCGCAG CGCCCGACCG 541 AAAGGAGCGC ACGACCCCAT GCATCGATGA TATCAGATCC CCGGGATGCA GAAATTGATG 601 ATCTATTAAA CAATAAAGAT GTCCACTAAA ATGGAAGTTT TTCCTGTCAT ACTTTGTTAA 661 GAAGGGTGAG AACAGAGTAC CTACATTTTG AATGGAAGGA TTGGAGCTAC GGGGGTGGGG 721 GTGGGGTGGG ATTAGATAAA TGCCTGCTCT TTACTGAAGG CTCTTTACTA TTGCTTTATG 781 ATAATGTTTC ATAGTTGGAT ATCATAATTT AAACAAGCAA AACCAAAATT AAGGGCCCAG 841 CTCATTCCTC CCACTCATGA TCTATAGATC CTATAGATCC TCTCGTGGGA TCATTGTTTT 901 TCTCTTTGAT TCCCACTTTG GTGGTTCTAA GTACTGTGGG TTTCCAAATG TGTCAGTTTT 961 CATAGGCCTG AAGAACGAGA TCAGCAGCCC TCTGTTCCAC ATACACTTCA ATTCTCAGTA 1021 TTGGTTTTTG CCAAGTTCTA AATTCCATCA GAAGCTGGTC GAGATCAGAC CCTTTAATAT 1081 AACTTCGGTA TAATGTATGC CTAATACGAA GTAATAAGGT TCCTCCAAGG AGGTTCCAAC 1141 TTAGGAATCC GGGATTCACC CAGAGCGTTC TTTG // |
|
| Primer: M13R | RDB13928_A6B8c_2.seq |
|
>D03070A12_A6B8_1_M13R_A03_03.ab1 1 CTCGCGCATT ACCCTCACTA AAGGGAACAA AAGCTGGGTA CCGGATCCTA GTGAACCTCT 61 TCGAGGGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATATTA AGGGTTCCGG 121 ATCTCGACCA GCTTCTGATG GAATTAGAAC TTGGCAAAAC AATACTGAGA ATGAAGTGTA 181 TGTGGAACAG AGGCTGCTGA TCTCGTTCTT CAGGCTATGA AACTGACACA TTTGGAAACC 241 ACAGTACTTA GAACCACAAA GTGGGAATCA AGAGAAAAAC AATGATCCCA CGAGAGATCT 301 ATAGATCTAT AGATCATGAG TGGGAGGAAT GAGCTGGCCC TTAATTTGGT TTTGCTTGTT 361 TAAATTATGA TATCCAACTA TGAAACATTA TCATAAAGCA ATAGTAAAGA GCCTTCAGTA 421 AAGAGCAGGC ATTTATCTAA TCCCACCCCA CCCCCACCCC CGTAGCTCCA ATCCTTCCAT 481 TCAAAATGTA GGTACTCTGT TCTCACCCTT CTTAACAAAG TATGACAGGA AAAACTTCCA 541 TTTTAGTGGA CATCTTTATT GTTTAATAGA TCATCAATTT CTGCATCCCG GGGATCTGAT 601 ATCATCGATG CATGGGGTCG TGCGCTCCTT TCGGTCGGGC GCTGCGGGTC GTGGGGCGGG 661 CGGCTAGCTT AATTTCGGGT ATATTTGAGT GGAATGAGTT CTTCAATCGT AGTTTTGACT 721 AACTTGCCAT TCATTTCTAT TAACACAAAA CAATCTGGTG CATAGTCTGA AATCAACTCC 781 CTACACATAC CACAAGGACT TACCACTCGA ATACTTCTAT CTACTTCGTC AGAATAAGGG 841 TGTCTAACAG CTACAATCGT GTCAAAATCC TTTTGTCCAT TCGAAACTGC ACTACCAATC 901 GCAATGGCTT CTGCACAAAC AGTTACTCGT CCTATATACG CTTCAATATG TACTGCCGAA 961 ATGATTTCTC CTGTTTTCGT ACGAATTGCC GCTCCCACAT GATGTTTATT TATCCTCATA 1021 AAGCATTGTA ATCTTCTCTG TCGCTACTTC TACTAATTCT AGATCTGTGA GAAATGTTAA 1081 ATGTTTTCAA TGCTAGCGTA GCCTCAGCCT GCTCGAAGAA TCCTAGATGG ATTGCCAAGG 1141 GTTCCGAAAT GC // |
|
Please visit Sequencing and PCR primers for primer information.
References
| original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
|---|
2018.09.03
GNP_filter3_RDBDEP_html_180823.pl