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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK285 (Stag-3FLAG-Bsr)

A plasmid for construction of C-terminal Stag-3FLAG tagging donors.

Catalog number RDB13928
Resource name pMK285 (Stag-3FLAG-Bsr)
Alternative name Stag-3FLAG-Bsr
Clone info. A plasmid for construction of C-terminal Stag-3FLAG tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Depositor|Developer Kanemaki, Masato |
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External Database

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
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            提供同意書 (MTA, 非営利学術目的用)[Word]
            提供同意書 (MTA, 営利目的用)[Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。

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            RDB13928 pMK285 (Stag-3FLAG-Bsr) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pMK285 (Stag-3FLAG-Bsr) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13928).

            Reference section:

            Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Auxin Inducible Degron (AID) System (English text)
            Featured content Auxin Inducible Degron (AID) System (Japanese text)
            Featured content Genome Editing (English text)
            Featured content Genome Editing (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13928_A6B8p1-4.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13_-40 (Pr0410)
            Region: T7 pro,insert 5'(Linker,S-Tag,3xFLAG,SV40 pA,loxP,mPGK pro)
            Sequence file: RDB13928_A6B8d.seq check
            >D03070A12_A6B8_1_M13(-40)_E03_15.ab1
                1 NNNNNNNNNC GCNNANNNGA NTNNCNATAG GGCGAATTGG AGCTCGGATC CGGTGCAGGC
               61 GCCAAGGAAA CCGCCGCTGC AAAATTCGAA CGCCAGCACA TGGACAGCCG ATCCCGGGCT
              121 GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT
              181 GACAAGTAGT GTACAAGTAA TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG
              241 AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA
              301 ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA
              361 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA
              421 AACTCATCAA TGTATCTTAA CGCGTCGATC ATATTCAATA ACCCTTAATA TAACTTCGTA
              481 TAATGTATGC TATACGAAGT TATTAGGTCT GAAGAGGAGT TTACGTCCAG CCAAGCTTAG
              541 GATCTCGACC TCGAAATTCT ACCGGGTAGG GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC
              601 ATGCGCTTTA GCAGCCCCGC TGGGCACTTG GCGCTACACA AGTGGCCTCT GGCCTCGCAC
              661 ACATTCCACA TCCACCGGTA GGCGCCAACC GACTCCGTTC TTTGGTGGCC CCTTCGCGCC
              721 ACCTTCTACT CCTCCCCTAG TCAGGAAGTT CCCCCCCGCC CCGCAGCTCG CGTCGTGCAN
              781 GANGTGACAA ATGGAAGTAG CACGTCTCAC TAGTCTCGTG CAGATGGACA GCACCGCTGA
              841 NCAATGGAAG CGGGTANGCC TTTGGGGNAG CGGCNAATAN CANCTTTGCT CCTTCNCTTT
              901 CTGGGNTCAN ANGCTGGGAN NGGGNGGNNN CGGGGGCGGG CTCNNGGGNG GGNTCNNNGC
              961 GGGGCGGGCG CCCGANNNNN NNCNNNNNNC NNNNANNNNG CNNGCNNCAA AAGCNNNNNT
            1021 NNNNNNNNNN
            //
            Primer: M13R (Pr0160)
            Region: insert 3'(loxP,mPGK ter,Bar,)
            Sequence file: RDB13928_A6B8e.seq check
            >D03070A12_A6B8_1_M13R_A03_03.ab1
                1 NNNNNNNNNN NNCNNNNNNN ANGGGNNNNA AAGCTGGGTA CCGGATCCTA GTGAACCTCT
               61 TCGAGGGANC TAATAACTTC GTATAGCATA CATTATACGA AGTTATATTA AGGGTTCCGG
              121 ATCTCGACCA GCTTCTGATG GAATTAGAAC TTGGCAAAAC AATACTGAGA ATGAAGTGTA
              181 TGTGGAACAG AGGCTGCTGA TCTCGTTCTT CAGGCTATGA AACTGACACA TTTGGAAACC
              241 ACAGTACTTA GAACCACAAA GTGGGAATCA AGAGAAAAAC AATGATCCCA CGAGAGATCT
              301 ATAGATCTAT AGATCATGAG TGGGAGGAAT GAGCTGGCCC TTAATTTGGT TTTGCTTGTT
              361 TAAATTATGA TATCCAACTA TGAAACATTA TCATAAAGCA ATAGTAAAGA GCCTTCAGTA
              421 AAGAGCAGGC ATTTATCTAA TCCCACCCCA CCCCCACCCC CGTAGCTCCA ATCCTTCCAT
              481 TCAAAATGTA GGTACTCTGT TCTCACCCTT CTTAACAAAG TATGACAGGA AAAACTTCCA
              541 TTTTAGTGGA CATCTTTATT GTTTAATAGA TCATCAATTT CTGCATCCCG GGGATCTGAT
              601 ATCATCGATG CATGGGGTCG TGCGCTCCTT TCGGTCGGGC GCTGCGGGTC GTGGGGCGGG
              661 CGGCTAGCTT AATTTCGGGT ATATTTGAGT GGAATGAGTT CTTCAATCGT AGTTTTGACT
              721 AACTTGCCAT TCATTTCTAT TAACACAAAA CAATCTGGTG CATAGTCTGA AATCAACTCC
              781 CTACACATAC NACAAGGACT TACCACTCGA ATACTTCTAT CTACTTCGTC AGAATAANGG
              841 TGTCTAACAG CTACAATCGT GTCAAAATCC TTTTGTCCAT TCGAAACTGC ACTACCAATC
              901 GCAATGGCTT CNGCACAAAC AGTTACTCGN CCTATATANN CTTCAANATG TACNGCCNAA
              961 ATGATTTNNC CNNNTTTCNT ACGANTNGNC NCNNCNNNNN NNNGNTNNNT NNNCCNNATN
            1021 ANNNNNNNNN ANNNNNNNNN N
            //
            Primer: PGKpro_F (Pr0249)
            Region: insert mid(Bsr)
            Sequence file: RDB13928_A6B8f.seq check
            >D03070A12_A6B8_1_PGK-proF_F07_16.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNTCCGGGCC TTTCGACCTG CATCCNTCTA GATCTCGAGC
               61 AGCTGAAGCT TACCGCTAGC ATGAAAACAT TTAACATTTC TCAACAAGAT CTAGAATTAG
              121 TAGAAGTAGC GACAGAGAAG ATTACAATGC TTTATGAGGA TAATAAACAT CATGTGGGAG
              181 CGGCAATTCG TACGAAAACA GGAGAAATCA TTTCGGCAGT ACATATTGAA GCGTATATAG
              241 GACGAGTAAC TGTTTGTGCA GAAGCCATTG CGATTGGTAG TGCAGTTTCG AATGGACAAA
              301 AGGATTTTGA CACGATTGTA GCTGTTAGAC ACCCTTATTC TGACGAAGTA GATAGAAGTA
              361 TTCGAGTGGT AAGTCCTTGT GGTATGTGTA GGGAGTTGAT TTCAGACTAT GCACCAGATT
              421 GTTTTGTGTT AATAGAAATG AATGGCAAGT TAGTCAAAAC TACGATTGAA GAACTCATTC
              481 CACTCAAATA TACCCGAAAT TAAGCTAGCC GCCCGCCCCA CGACCCGCAG CGCCCGACCG
              541 AAAGGAGCGC ACGACCCCAT GCATCGATGA TATCAGATCC CCGGGATGCA GAAATTGATG
              601 ATCTATTAAA CAATAAAGAT GTCCACTAAA ATGGAAGTTT TTCCTGTCAT ACTTTGTTAA
              661 GAAGGGTGAG AACAGAGTAC CTACATTTTG AATGGAAGGA TTGGAGCTAC GGGGGTGGGG
              721 GTGGGGTGGG ANTAGATAAA TGCCTGCTCT TTACTGAAGG CTCTTTACTA TTGCTTTATG
              781 ATAATGTTTC NTANTTGGAT ATCATAATTT AAACNAGCAA AACCNAANTT AAGGGNCNNG
              841 CTCATTCCTC CCACTCATGA TCNANNNNTN NNANAGATNN NCTCNTGGGA TNNNTGTTTT
              901 TCNCNTNGAT TCCCNNTTNN NNGGTTCTAA NTACNGTGGN TTNNCAAATG TGTCANNTTT
              961 CNNANNCNTG ANNANNGANN TNNGCANNCC TCNNNNNCNN NNACNNTTNN NNNNTNNNNA
            1021 NNNGNTTTNN NNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP]

            2023.10.05

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