Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK285 (Stag-3FLAG-Bsr)

A plasmid for construction of C-terminal Stag-3FLAG tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13928_A6B8p1_v2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB13928_A6B8a_2.seq
>D03070A12_A6B8_1_M13(-40)_E03_15.ab1     1 AAGCGGACGC GCGTATACGA CTCACTATAG GGCGAATTGG AGCTCGGATC CGGTGCAGGC    61 GCCAAGGAAA CCGCCGCTGC AAAATTCGAA CGCCAGCACA TGGACAGCCG ATCCCGGGCT   121 GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT   181 GACAAGTAGT GTACAAGTAA TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG   241 AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA   301 ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA   361 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA   421 AACTCATCAA TGTATCTTAA CGCGTCGATC ATATTCAATA ACCCTTAATA TAACTTCGTA   481 TAATGTATGC TATACGAAGT TATTAGGTCT GAAGAGGAGT TTACGTCCAG CCAAGCTTAG   541 GATCTCGACC TCGAAATTCT ACCGGGTAGG GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC   601 ATGCGCTTTA GCAGCCCCGC TGGGCACTTG GCGCTACACA AGTGGCCTCT GGCCTCGCAC   661 ACATTCCACA TCCACCGGTA GGCGCCAACC GACTCCGTTC TTTGGTGGCC CCTTCGCGCC   721 ACCTTCTACT CCTCCCCTAG TCAGGAAGTT CCCCCCCGCC CCGCAGCTCG CGTCGTGCAG   781 GACGTGACAA ATGGAAGTAG CACGTCTCAC TAGTCTCGTG CAGATGGACA GCACCGCTGA   841 GCAATGGAAG CGGGTAGGCC TTTGGGGCAG CGGCCAATAG CAGCTTTGCT CCTTCGCTTT   901 CTGGGCTCAG AGGCTGGGAA GGGGTGGGTC CGGGGGCGGG CTCAGGGGCG GGCTCAGGGC   961 GGGGCGGGCG CCCGAAGTCC TCCCGAGGCC CGGCATTCTG CACGCTTCAA AAGCGCACGT  1021 CTGCCGCGCT GTTCTCCTCT TCCTCATCTC CGGGCCTTTC GACTGCATCC ATCTAGATCT  1081 CGAGCAGCTG AAGCTACGCT AGCATGAAAA CATTACCATT TCTCACCAGA TCTAGATAAG 1141 TAGGAGTAAG CGGACAGAGA CAAGAATTAC A //
Primer: PGKpro_F Sequence file: RDB13928_A6B8b_2.seq
>D03070A12_A6B8_1_PGK-proF_F07_16.ab1     1 AGGGGCGTTC TCTCTTCTCA TCTCCGGGCC TTTCGACCTG CATCCATCTA GATCTCGAGC    61 AGCTGAAGCT TACCGCTAGC ATGAAAACAT TTAACATTTC TCAACAAGAT CTAGAATTAG   121 TAGAAGTAGC GACAGAGAAG ATTACAATGC TTTATGAGGA TAATAAACAT CATGTGGGAG   181 CGGCAATTCG TACGAAAACA GGAGAAATCA TTTCGGCAGT ACATATTGAA GCGTATATAG   241 GACGAGTAAC TGTTTGTGCA GAAGCCATTG CGATTGGTAG TGCAGTTTCG AATGGACAAA   301 AGGATTTTGA CACGATTGTA GCTGTTAGAC ACCCTTATTC TGACGAAGTA GATAGAAGTA   361 TTCGAGTGGT AAGTCCTTGT GGTATGTGTA GGGAGTTGAT TTCAGACTAT GCACCAGATT   421 GTTTTGTGTT AATAGAAATG AATGGCAAGT TAGTCAAAAC TACGATTGAA GAACTCATTC   481 CACTCAAATA TACCCGAAAT TAAGCTAGCC GCCCGCCCCA CGACCCGCAG CGCCCGACCG   541 AAAGGAGCGC ACGACCCCAT GCATCGATGA TATCAGATCC CCGGGATGCA GAAATTGATG   601 ATCTATTAAA CAATAAAGAT GTCCACTAAA ATGGAAGTTT TTCCTGTCAT ACTTTGTTAA   661 GAAGGGTGAG AACAGAGTAC CTACATTTTG AATGGAAGGA TTGGAGCTAC GGGGGTGGGG   721 GTGGGGTGGG ATTAGATAAA TGCCTGCTCT TTACTGAAGG CTCTTTACTA TTGCTTTATG   781 ATAATGTTTC ATAGTTGGAT ATCATAATTT AAACAAGCAA AACCAAAATT AAGGGCCCAG   841 CTCATTCCTC CCACTCATGA TCTATAGATC CTATAGATCC TCTCGTGGGA TCATTGTTTT   901 TCTCTTTGAT TCCCACTTTG GTGGTTCTAA GTACTGTGGG TTTCCAAATG TGTCAGTTTT   961 CATAGGCCTG AAGAACGAGA TCAGCAGCCC TCTGTTCCAC ATACACTTCA ATTCTCAGTA  1021 TTGGTTTTTG CCAAGTTCTA AATTCCATCA GAAGCTGGTC GAGATCAGAC CCTTTAATAT  1081 AACTTCGGTA TAATGTATGC CTAATACGAA GTAATAAGGT TCCTCCAAGG AGGTTCCAAC 1141 TTAGGAATCC GGGATTCACC CAGAGCGTTC TTTG //
Primer: M13R Sequence file: RDB13928_A6B8c_2.seq
>D03070A12_A6B8_1_M13R_A03_03.ab1     1 CTCGCGCATT ACCCTCACTA AAGGGAACAA AAGCTGGGTA CCGGATCCTA GTGAACCTCT    61 TCGAGGGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATATTA AGGGTTCCGG   121 ATCTCGACCA GCTTCTGATG GAATTAGAAC TTGGCAAAAC AATACTGAGA ATGAAGTGTA   181 TGTGGAACAG AGGCTGCTGA TCTCGTTCTT CAGGCTATGA AACTGACACA TTTGGAAACC   241 ACAGTACTTA GAACCACAAA GTGGGAATCA AGAGAAAAAC AATGATCCCA CGAGAGATCT   301 ATAGATCTAT AGATCATGAG TGGGAGGAAT GAGCTGGCCC TTAATTTGGT TTTGCTTGTT   361 TAAATTATGA TATCCAACTA TGAAACATTA TCATAAAGCA ATAGTAAAGA GCCTTCAGTA   421 AAGAGCAGGC ATTTATCTAA TCCCACCCCA CCCCCACCCC CGTAGCTCCA ATCCTTCCAT   481 TCAAAATGTA GGTACTCTGT TCTCACCCTT CTTAACAAAG TATGACAGGA AAAACTTCCA   541 TTTTAGTGGA CATCTTTATT GTTTAATAGA TCATCAATTT CTGCATCCCG GGGATCTGAT   601 ATCATCGATG CATGGGGTCG TGCGCTCCTT TCGGTCGGGC GCTGCGGGTC GTGGGGCGGG   661 CGGCTAGCTT AATTTCGGGT ATATTTGAGT GGAATGAGTT CTTCAATCGT AGTTTTGACT   721 AACTTGCCAT TCATTTCTAT TAACACAAAA CAATCTGGTG CATAGTCTGA AATCAACTCC   781 CTACACATAC CACAAGGACT TACCACTCGA ATACTTCTAT CTACTTCGTC AGAATAAGGG   841 TGTCTAACAG CTACAATCGT GTCAAAATCC TTTTGTCCAT TCGAAACTGC ACTACCAATC   901 GCAATGGCTT CTGCACAAAC AGTTACTCGT CCTATATACG CTTCAATATG TACTGCCGAA   961 ATGATTTCTC CTGTTTTCGT ACGAATTGCC GCTCCCACAT GATGTTTATT TATCCTCATA  1021 AAGCATTGTA ATCTTCTCTG TCGCTACTTC TACTAATTCT AGATCTGTGA GAAATGTTAA  1081 ATGTTTTCAA TGCTAGCGTA GCCTCAGCCT GCTCGAAGAA TCCTAGATGG ATTGCCAAGG 1141 GTTCCGAAAT GC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.02.09

GNP_filter3_RDBDEP_html_220112.pl