Resource data sheet
Prev.

pMK285 (Stag-3FLAG-Bsr)

A plasmid for construction of C-terminal Stag-3FLAG tagging donors.

Catalog number RDB13928
Resource name pMK285 (Stag-3FLAG-Bsr)
Alternative name Stag-3FLAG-Bsr
Clone info. A plasmid for construction of C-terminal Stag-3FLAG tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Depositor Kanemaki, Masato |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13928 pMK285 (Stag-3FLAG-Bsr) DNA solution

cDNA Request [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)
Featured content Genome Editing (English text)
Featured content Genome Editing (Japanese text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

References

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13928_A6B8p1_v2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB13928_A6B8a_2.seq
>D03070A12_A6B8_1_M13(-40)_E03_15.ab1
    1 AAGCGGACGC GCGTATACGA CTCACTATAG GGCGAATTGG AGCTCGGATC CGGTGCAGGC
   61 GCCAAGGAAA CCGCCGCTGC AAAATTCGAA CGCCAGCACA TGGACAGCCG ATCCCGGGCT
  121 GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT
  181 GACAAGTAGT GTACAAGTAA TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG
  241 AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA
  301 ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA
  361 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA
  421 AACTCATCAA TGTATCTTAA CGCGTCGATC ATATTCAATA ACCCTTAATA TAACTTCGTA
  481 TAATGTATGC TATACGAAGT TATTAGGTCT GAAGAGGAGT TTACGTCCAG CCAAGCTTAG
  541 GATCTCGACC TCGAAATTCT ACCGGGTAGG GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC
  601 ATGCGCTTTA GCAGCCCCGC TGGGCACTTG GCGCTACACA AGTGGCCTCT GGCCTCGCAC
  661 ACATTCCACA TCCACCGGTA GGCGCCAACC GACTCCGTTC TTTGGTGGCC CCTTCGCGCC
  721 ACCTTCTACT CCTCCCCTAG TCAGGAAGTT CCCCCCCGCC CCGCAGCTCG CGTCGTGCAG
  781 GACGTGACAA ATGGAAGTAG CACGTCTCAC TAGTCTCGTG CAGATGGACA GCACCGCTGA
  841 GCAATGGAAG CGGGTAGGCC TTTGGGGCAG CGGCCAATAG CAGCTTTGCT CCTTCGCTTT
  901 CTGGGCTCAG AGGCTGGGAA GGGGTGGGTC CGGGGGCGGG CTCAGGGGCG GGCTCAGGGC
  961 GGGGCGGGCG CCCGAAGTCC TCCCGAGGCC CGGCATTCTG CACGCTTCAA AAGCGCACGT
 1021 CTGCCGCGCT GTTCTCCTCT TCCTCATCTC CGGGCCTTTC GACTGCATCC ATCTAGATCT
 1081 CGAGCAGCTG AAGCTACGCT AGCATGAAAA CATTACCATT TCTCACCAGA TCTAGATAAG
1141 TAGGAGTAAG CGGACAGAGA CAAGAATTAC A
//
Primer: PGKpro_F RDB13928_A6B8b_2.seq
>D03070A12_A6B8_1_PGK-proF_F07_16.ab1
    1 AGGGGCGTTC TCTCTTCTCA TCTCCGGGCC TTTCGACCTG CATCCATCTA GATCTCGAGC
   61 AGCTGAAGCT TACCGCTAGC ATGAAAACAT TTAACATTTC TCAACAAGAT CTAGAATTAG
  121 TAGAAGTAGC GACAGAGAAG ATTACAATGC TTTATGAGGA TAATAAACAT CATGTGGGAG
  181 CGGCAATTCG TACGAAAACA GGAGAAATCA TTTCGGCAGT ACATATTGAA GCGTATATAG
  241 GACGAGTAAC TGTTTGTGCA GAAGCCATTG CGATTGGTAG TGCAGTTTCG AATGGACAAA
  301 AGGATTTTGA CACGATTGTA GCTGTTAGAC ACCCTTATTC TGACGAAGTA GATAGAAGTA
  361 TTCGAGTGGT AAGTCCTTGT GGTATGTGTA GGGAGTTGAT TTCAGACTAT GCACCAGATT
  421 GTTTTGTGTT AATAGAAATG AATGGCAAGT TAGTCAAAAC TACGATTGAA GAACTCATTC
  481 CACTCAAATA TACCCGAAAT TAAGCTAGCC GCCCGCCCCA CGACCCGCAG CGCCCGACCG
  541 AAAGGAGCGC ACGACCCCAT GCATCGATGA TATCAGATCC CCGGGATGCA GAAATTGATG
  601 ATCTATTAAA CAATAAAGAT GTCCACTAAA ATGGAAGTTT TTCCTGTCAT ACTTTGTTAA
  661 GAAGGGTGAG AACAGAGTAC CTACATTTTG AATGGAAGGA TTGGAGCTAC GGGGGTGGGG
  721 GTGGGGTGGG ATTAGATAAA TGCCTGCTCT TTACTGAAGG CTCTTTACTA TTGCTTTATG
  781 ATAATGTTTC ATAGTTGGAT ATCATAATTT AAACAAGCAA AACCAAAATT AAGGGCCCAG
  841 CTCATTCCTC CCACTCATGA TCTATAGATC CTATAGATCC TCTCGTGGGA TCATTGTTTT
  901 TCTCTTTGAT TCCCACTTTG GTGGTTCTAA GTACTGTGGG TTTCCAAATG TGTCAGTTTT
  961 CATAGGCCTG AAGAACGAGA TCAGCAGCCC TCTGTTCCAC ATACACTTCA ATTCTCAGTA
 1021 TTGGTTTTTG CCAAGTTCTA AATTCCATCA GAAGCTGGTC GAGATCAGAC CCTTTAATAT
 1081 AACTTCGGTA TAATGTATGC CTAATACGAA GTAATAAGGT TCCTCCAAGG AGGTTCCAAC
1141 TTAGGAATCC GGGATTCACC CAGAGCGTTC TTTG
//
Primer: M13R RDB13928_A6B8c_2.seq
>D03070A12_A6B8_1_M13R_A03_03.ab1
    1 CTCGCGCATT ACCCTCACTA AAGGGAACAA AAGCTGGGTA CCGGATCCTA GTGAACCTCT
   61 TCGAGGGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATATTA AGGGTTCCGG
  121 ATCTCGACCA GCTTCTGATG GAATTAGAAC TTGGCAAAAC AATACTGAGA ATGAAGTGTA
  181 TGTGGAACAG AGGCTGCTGA TCTCGTTCTT CAGGCTATGA AACTGACACA TTTGGAAACC
  241 ACAGTACTTA GAACCACAAA GTGGGAATCA AGAGAAAAAC AATGATCCCA CGAGAGATCT
  301 ATAGATCTAT AGATCATGAG TGGGAGGAAT GAGCTGGCCC TTAATTTGGT TTTGCTTGTT
  361 TAAATTATGA TATCCAACTA TGAAACATTA TCATAAAGCA ATAGTAAAGA GCCTTCAGTA
  421 AAGAGCAGGC ATTTATCTAA TCCCACCCCA CCCCCACCCC CGTAGCTCCA ATCCTTCCAT
  481 TCAAAATGTA GGTACTCTGT TCTCACCCTT CTTAACAAAG TATGACAGGA AAAACTTCCA
  541 TTTTAGTGGA CATCTTTATT GTTTAATAGA TCATCAATTT CTGCATCCCG GGGATCTGAT
  601 ATCATCGATG CATGGGGTCG TGCGCTCCTT TCGGTCGGGC GCTGCGGGTC GTGGGGCGGG
  661 CGGCTAGCTT AATTTCGGGT ATATTTGAGT GGAATGAGTT CTTCAATCGT AGTTTTGACT
  721 AACTTGCCAT TCATTTCTAT TAACACAAAA CAATCTGGTG CATAGTCTGA AATCAACTCC
  781 CTACACATAC CACAAGGACT TACCACTCGA ATACTTCTAT CTACTTCGTC AGAATAAGGG
  841 TGTCTAACAG CTACAATCGT GTCAAAATCC TTTTGTCCAT TCGAAACTGC ACTACCAATC
  901 GCAATGGCTT CTGCACAAAC AGTTACTCGT CCTATATACG CTTCAATATG TACTGCCGAA
  961 ATGATTTCTC CTGTTTTCGT ACGAATTGCC GCTCCCACAT GATGTTTATT TATCCTCATA
 1021 AAGCATTGTA ATCTTCTCTG TCGCTACTTC TACTAATTCT AGATCTGTGA GAAATGTTAA
 1081 ATGTTTTCAA TGCTAGCGTA GCCTCAGCCT GCTCGAAGAA TCCTAGATGG ATTGCCAAGG
1141 GTTCCGAAAT GC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

2018.09.03

GNP_filter3_RDBDEP_html_180823.pl