Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK285 (Stag-3FLAG-Bsr) (#RDB13928)

A plasmid for construction of C-terminal Stag-3FLAG tagging donors.

Alternative name Stag-3FLAG-Bsr
Clone info. A plasmid for construction of C-terminal Stag-3FLAG tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Depositor|Developer Kanemaki, Masato |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13928 pMK285 (Stag-3FLAG-Bsr) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK285 (Stag-3FLAG-Bsr) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13928).

Reference section:

Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13928_A6B8p1-4.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410)
Region: T7 pro,insert 5'(Linker,S-Tag,3xFLAG,SV40 pA,loxP,mPGK pro)
Sequence file: RDB13928_A6B8d.seq check
>D03070A12_A6B8_1_M13(-40)_E03_15.ab1
    1 NNNNNNNNNC GCNNANNNGA NTNNCNATAG GGCGAATTGG AGCTCGGATC CGGTGCAGGC
   61 GCCAAGGAAA CCGCCGCTGC AAAATTCGAA CGCCAGCACA TGGACAGCCG ATCCCGGGCT
  121 GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT
  181 GACAAGTAGT GTACAAGTAA TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG
  241 AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA
  301 ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA
  361 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA
  421 AACTCATCAA TGTATCTTAA CGCGTCGATC ATATTCAATA ACCCTTAATA TAACTTCGTA
  481 TAATGTATGC TATACGAAGT TATTAGGTCT GAAGAGGAGT TTACGTCCAG CCAAGCTTAG
  541 GATCTCGACC TCGAAATTCT ACCGGGTAGG GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC
  601 ATGCGCTTTA GCAGCCCCGC TGGGCACTTG GCGCTACACA AGTGGCCTCT GGCCTCGCAC
  661 ACATTCCACA TCCACCGGTA GGCGCCAACC GACTCCGTTC TTTGGTGGCC CCTTCGCGCC
  721 ACCTTCTACT CCTCCCCTAG TCAGGAAGTT CCCCCCCGCC CCGCAGCTCG CGTCGTGCAN
  781 GANGTGACAA ATGGAAGTAG CACGTCTCAC TAGTCTCGTG CAGATGGACA GCACCGCTGA
  841 NCAATGGAAG CGGGTANGCC TTTGGGGNAG CGGCNAATAN CANCTTTGCT CCTTCNCTTT
  901 CTGGGNTCAN ANGCTGGGAN NGGGNGGNNN CGGGGGCGGG CTCNNGGGNG GGNTCNNNGC
  961 GGGGCGGGCG CCCGANNNNN NNCNNNNNNC NNNNANNNNG CNNGCNNCAA AAGCNNNNNT
1021 NNNNNNNNNN
//
Primer: M13R (Pr0160)
Region: insert 3'(loxP,mPGK ter,Bar,)
Sequence file: RDB13928_A6B8e.seq check
>D03070A12_A6B8_1_M13R_A03_03.ab1
    1 NNNNNNNNNN NNCNNNNNNN ANGGGNNNNA AAGCTGGGTA CCGGATCCTA GTGAACCTCT
   61 TCGAGGGANC TAATAACTTC GTATAGCATA CATTATACGA AGTTATATTA AGGGTTCCGG
  121 ATCTCGACCA GCTTCTGATG GAATTAGAAC TTGGCAAAAC AATACTGAGA ATGAAGTGTA
  181 TGTGGAACAG AGGCTGCTGA TCTCGTTCTT CAGGCTATGA AACTGACACA TTTGGAAACC
  241 ACAGTACTTA GAACCACAAA GTGGGAATCA AGAGAAAAAC AATGATCCCA CGAGAGATCT
  301 ATAGATCTAT AGATCATGAG TGGGAGGAAT GAGCTGGCCC TTAATTTGGT TTTGCTTGTT
  361 TAAATTATGA TATCCAACTA TGAAACATTA TCATAAAGCA ATAGTAAAGA GCCTTCAGTA
  421 AAGAGCAGGC ATTTATCTAA TCCCACCCCA CCCCCACCCC CGTAGCTCCA ATCCTTCCAT
  481 TCAAAATGTA GGTACTCTGT TCTCACCCTT CTTAACAAAG TATGACAGGA AAAACTTCCA
  541 TTTTAGTGGA CATCTTTATT GTTTAATAGA TCATCAATTT CTGCATCCCG GGGATCTGAT
  601 ATCATCGATG CATGGGGTCG TGCGCTCCTT TCGGTCGGGC GCTGCGGGTC GTGGGGCGGG
  661 CGGCTAGCTT AATTTCGGGT ATATTTGAGT GGAATGAGTT CTTCAATCGT AGTTTTGACT
  721 AACTTGCCAT TCATTTCTAT TAACACAAAA CAATCTGGTG CATAGTCTGA AATCAACTCC
  781 CTACACATAC NACAAGGACT TACCACTCGA ATACTTCTAT CTACTTCGTC AGAATAANGG
  841 TGTCTAACAG CTACAATCGT GTCAAAATCC TTTTGTCCAT TCGAAACTGC ACTACCAATC
  901 GCAATGGCTT CNGCACAAAC AGTTACTCGN CCTATATANN CTTCAANATG TACNGCCNAA
  961 ATGATTTNNC CNNNTTTCNT ACGANTNGNC NCNNCNNNNN NNNGNTNNNT NNNCCNNATN
1021 ANNNNNNNNN ANNNNNNNNN N
//
Primer: PGKpro_F (Pr0249)
Region: insert mid(Bsr)
Sequence file: RDB13928_A6B8f.seq check
>D03070A12_A6B8_1_PGK-proF_F07_16.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTCCGGGCC TTTCGACCTG CATCCNTCTA GATCTCGAGC
   61 AGCTGAAGCT TACCGCTAGC ATGAAAACAT TTAACATTTC TCAACAAGAT CTAGAATTAG
  121 TAGAAGTAGC GACAGAGAAG ATTACAATGC TTTATGAGGA TAATAAACAT CATGTGGGAG
  181 CGGCAATTCG TACGAAAACA GGAGAAATCA TTTCGGCAGT ACATATTGAA GCGTATATAG
  241 GACGAGTAAC TGTTTGTGCA GAAGCCATTG CGATTGGTAG TGCAGTTTCG AATGGACAAA
  301 AGGATTTTGA CACGATTGTA GCTGTTAGAC ACCCTTATTC TGACGAAGTA GATAGAAGTA
  361 TTCGAGTGGT AAGTCCTTGT GGTATGTGTA GGGAGTTGAT TTCAGACTAT GCACCAGATT
  421 GTTTTGTGTT AATAGAAATG AATGGCAAGT TAGTCAAAAC TACGATTGAA GAACTCATTC
  481 CACTCAAATA TACCCGAAAT TAAGCTAGCC GCCCGCCCCA CGACCCGCAG CGCCCGACCG
  541 AAAGGAGCGC ACGACCCCAT GCATCGATGA TATCAGATCC CCGGGATGCA GAAATTGATG
  601 ATCTATTAAA CAATAAAGAT GTCCACTAAA ATGGAAGTTT TTCCTGTCAT ACTTTGTTAA
  661 GAAGGGTGAG AACAGAGTAC CTACATTTTG AATGGAAGGA TTGGAGCTAC GGGGGTGGGG
  721 GTGGGGTGGG ANTAGATAAA TGCCTGCTCT TTACTGAAGG CTCTTTACTA TTGCTTTATG
  781 ATAATGTTTC NTANTTGGAT ATCATAATTT AAACNAGCAA AACCNAANTT AAGGGNCNNG
  841 CTCATTCCTC CCACTCATGA TCNANNNNTN NNANAGATNN NCTCNTGGGA TNNNTGTTTT
  901 TCNCNTNGAT TCCCNNTTNN NNGGTTCTAA NTACNGTGGN TTNNCAAATG TGTCANNTTT
  961 CNNANNCNTG ANNANNGANN TNNGCANNCC TCNNNNNCNN NNACNNTTNN NNNNTNNNNA
1021 NNNGNTTTNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP]