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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK285 (Stag-3FLAG-Bsr)

A plasmid for construction of C-terminal Stag-3FLAG tagging donors.

Catalog number RDB13928
Resource name pMK285 (Stag-3FLAG-Bsr)
Alternative name Stag-3FLAG-Bsr
Clone info. A plasmid for construction of C-terminal Stag-3FLAG tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Depositor|Developer Kanemaki, Masato |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB13928 pMK285 (Stag-3FLAG-Bsr) DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Auxin Inducible Degron (AID) System (English text)
            Featured content Auxin Inducible Degron (AID) System (Japanese text)
            Featured content Genome Editing (English text)
            Featured content Genome Editing (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13928_A6B8p1_v2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13_-40
            Sequence file: RDB13928_A6B8a_2.seq check
            >D03070A12_A6B8_1_M13(-40)_E03_15.ab1
                1 AAGCGGACGC GCGTATACGA CTCACTATAG GGCGAATTGG AGCTCGGATC CGGTGCAGGC
               61 GCCAAGGAAA CCGCCGCTGC AAAATTCGAA CGCCAGCACA TGGACAGCCG ATCCCGGGCT
              121 GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT
              181 GACAAGTAGT GTACAAGTAA TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG
              241 AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA
              301 ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA
              361 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA
              421 AACTCATCAA TGTATCTTAA CGCGTCGATC ATATTCAATA ACCCTTAATA TAACTTCGTA
              481 TAATGTATGC TATACGAAGT TATTAGGTCT GAAGAGGAGT TTACGTCCAG CCAAGCTTAG
              541 GATCTCGACC TCGAAATTCT ACCGGGTAGG GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC
              601 ATGCGCTTTA GCAGCCCCGC TGGGCACTTG GCGCTACACA AGTGGCCTCT GGCCTCGCAC
              661 ACATTCCACA TCCACCGGTA GGCGCCAACC GACTCCGTTC TTTGGTGGCC CCTTCGCGCC
              721 ACCTTCTACT CCTCCCCTAG TCAGGAAGTT CCCCCCCGCC CCGCAGCTCG CGTCGTGCAG
              781 GACGTGACAA ATGGAAGTAG CACGTCTCAC TAGTCTCGTG CAGATGGACA GCACCGCTGA
              841 GCAATGGAAG CGGGTAGGCC TTTGGGGCAG CGGCCAATAG CAGCTTTGCT CCTTCGCTTT
              901 CTGGGCTCAG AGGCTGGGAA GGGGTGGGTC CGGGGGCGGG CTCAGGGGCG GGCTCAGGGC
              961 GGGGCGGGCG CCCGAAGTCC TCCCGAGGCC CGGCATTCTG CACGCTTCAA AAGCGCACGT
             1021 CTGCCGCGCT GTTCTCCTCT TCCTCATCTC CGGGCCTTTC GACTGCATCC ATCTAGATCT
             1081 CGAGCAGCTG AAGCTACGCT AGCATGAAAA CATTACCATT TCTCACCAGA TCTAGATAAG
            1141 TAGGAGTAAG CGGACAGAGA CAAGAATTAC A
            //
            Primer: PGKpro_F
            Sequence file: RDB13928_A6B8b_2.seq check
            >D03070A12_A6B8_1_PGK-proF_F07_16.ab1
                1 AGGGGCGTTC TCTCTTCTCA TCTCCGGGCC TTTCGACCTG CATCCATCTA GATCTCGAGC
               61 AGCTGAAGCT TACCGCTAGC ATGAAAACAT TTAACATTTC TCAACAAGAT CTAGAATTAG
              121 TAGAAGTAGC GACAGAGAAG ATTACAATGC TTTATGAGGA TAATAAACAT CATGTGGGAG
              181 CGGCAATTCG TACGAAAACA GGAGAAATCA TTTCGGCAGT ACATATTGAA GCGTATATAG
              241 GACGAGTAAC TGTTTGTGCA GAAGCCATTG CGATTGGTAG TGCAGTTTCG AATGGACAAA
              301 AGGATTTTGA CACGATTGTA GCTGTTAGAC ACCCTTATTC TGACGAAGTA GATAGAAGTA
              361 TTCGAGTGGT AAGTCCTTGT GGTATGTGTA GGGAGTTGAT TTCAGACTAT GCACCAGATT
              421 GTTTTGTGTT AATAGAAATG AATGGCAAGT TAGTCAAAAC TACGATTGAA GAACTCATTC
              481 CACTCAAATA TACCCGAAAT TAAGCTAGCC GCCCGCCCCA CGACCCGCAG CGCCCGACCG
              541 AAAGGAGCGC ACGACCCCAT GCATCGATGA TATCAGATCC CCGGGATGCA GAAATTGATG
              601 ATCTATTAAA CAATAAAGAT GTCCACTAAA ATGGAAGTTT TTCCTGTCAT ACTTTGTTAA
              661 GAAGGGTGAG AACAGAGTAC CTACATTTTG AATGGAAGGA TTGGAGCTAC GGGGGTGGGG
              721 GTGGGGTGGG ATTAGATAAA TGCCTGCTCT TTACTGAAGG CTCTTTACTA TTGCTTTATG
              781 ATAATGTTTC ATAGTTGGAT ATCATAATTT AAACAAGCAA AACCAAAATT AAGGGCCCAG
              841 CTCATTCCTC CCACTCATGA TCTATAGATC CTATAGATCC TCTCGTGGGA TCATTGTTTT
              901 TCTCTTTGAT TCCCACTTTG GTGGTTCTAA GTACTGTGGG TTTCCAAATG TGTCAGTTTT
              961 CATAGGCCTG AAGAACGAGA TCAGCAGCCC TCTGTTCCAC ATACACTTCA ATTCTCAGTA
             1021 TTGGTTTTTG CCAAGTTCTA AATTCCATCA GAAGCTGGTC GAGATCAGAC CCTTTAATAT
             1081 AACTTCGGTA TAATGTATGC CTAATACGAA GTAATAAGGT TCCTCCAAGG AGGTTCCAAC
            1141 TTAGGAATCC GGGATTCACC CAGAGCGTTC TTTG
            //
            Primer: M13R
            Sequence file: RDB13928_A6B8c_2.seq check
            >D03070A12_A6B8_1_M13R_A03_03.ab1
                1 CTCGCGCATT ACCCTCACTA AAGGGAACAA AAGCTGGGTA CCGGATCCTA GTGAACCTCT
               61 TCGAGGGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATATTA AGGGTTCCGG
              121 ATCTCGACCA GCTTCTGATG GAATTAGAAC TTGGCAAAAC AATACTGAGA ATGAAGTGTA
              181 TGTGGAACAG AGGCTGCTGA TCTCGTTCTT CAGGCTATGA AACTGACACA TTTGGAAACC
              241 ACAGTACTTA GAACCACAAA GTGGGAATCA AGAGAAAAAC AATGATCCCA CGAGAGATCT
              301 ATAGATCTAT AGATCATGAG TGGGAGGAAT GAGCTGGCCC TTAATTTGGT TTTGCTTGTT
              361 TAAATTATGA TATCCAACTA TGAAACATTA TCATAAAGCA ATAGTAAAGA GCCTTCAGTA
              421 AAGAGCAGGC ATTTATCTAA TCCCACCCCA CCCCCACCCC CGTAGCTCCA ATCCTTCCAT
              481 TCAAAATGTA GGTACTCTGT TCTCACCCTT CTTAACAAAG TATGACAGGA AAAACTTCCA
              541 TTTTAGTGGA CATCTTTATT GTTTAATAGA TCATCAATTT CTGCATCCCG GGGATCTGAT
              601 ATCATCGATG CATGGGGTCG TGCGCTCCTT TCGGTCGGGC GCTGCGGGTC GTGGGGCGGG
              661 CGGCTAGCTT AATTTCGGGT ATATTTGAGT GGAATGAGTT CTTCAATCGT AGTTTTGACT
              721 AACTTGCCAT TCATTTCTAT TAACACAAAA CAATCTGGTG CATAGTCTGA AATCAACTCC
              781 CTACACATAC CACAAGGACT TACCACTCGA ATACTTCTAT CTACTTCGTC AGAATAAGGG
              841 TGTCTAACAG CTACAATCGT GTCAAAATCC TTTTGTCCAT TCGAAACTGC ACTACCAATC
              901 GCAATGGCTT CTGCACAAAC AGTTACTCGT CCTATATACG CTTCAATATG TACTGCCGAA
              961 ATGATTTCTC CTGTTTTCGT ACGAATTGCC GCTCCCACAT GATGTTTATT TATCCTCATA
             1021 AAGCATTGTA ATCTTCTCTG TCGCTACTTC TACTAATTCT AGATCTGTGA GAAATGTTAA
             1081 ATGTTTTCAA TGCTAGCGTA GCCTCAGCCT GCTCGAAGAA TCCTAGATGG ATTGCCAAGG
            1141 GTTCCGAAAT GC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl