Resource data sheet
Prev.

pMK375 (Hygro-P2A-Stag-3FLAG)

A plasmid for construction of N-terminal Hygro-P2A-Stag-3FLAG tagging donors.

Catalog number RDB16813
Resource name pMK375 (Hygro-P2A-Stag-3FLAG)
Clone info. A plasmid for construction of N-terminal Hygro-P2A-Stag-3FLAG tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Depositor Kanemaki, Masato |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16813 pMK375 (Hygro-P2A-Stag-3FLAG) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16813_A8Lpp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 RDB16813_A8Lpa.seq
>D06157A9_A8LP_1_pUC-M13_-100_A05_02_ABI24.ab1
    1 GGATGTTCAA GGCGAATTAG GTTGGGTACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA
   61 ACGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGG TACCGGGCCC
  121 CCCCTCGAGG TCGACGGTAT CGATAAGCTT GATATCGCCA CCATGAAAAA GCCTGAACTC
  181 ACCGCGACGT CTGTCGAGAA GTTTCTGATC GAAAAGTTCG ACAGCGTCTC CGACCTGATG
  241 CAGCTCTCGG AGGGCGAAGA ATCTCGTGCT TTCAGCTTCG ATGTAGGAGG GCGTGGATAT
  301 GTCCTGCGGG TAAATAGCTG CGCCGATGGT TTCTACAAAG ATCGTTATGT TTATCGGCAC
  361 TTTGCATCGG CCGCGCTCCC GATTCCGGAA GTGCTTGACA TTGGGGAATT CAGCGAGAGC
  421 CTGACCTATT GCATCTCCCG CCGTGCACAG GGTGTCACGT TGCAAGACCT GCCTGAAACC
  481 GAACTGCCCG CTGTTCTGCA GCCGGTCGCG GAGGCCATGG ATGCGATCGC TGCGGCCGAT
  541 CTTAGCCAGA CGAGCGGGTT CGGCCCATTC GGACCGCAAG GAATCGGTCA ATACACTACA
  601 TGGCGTGATT TCATATGCGC GATTGCTGAT CCCCATGTGT ATCACTGGCA AACTGTGATG
  661 GACGACACCG TCAGTGCGTC CGTCGCGCAG GCTCTCGATG AGCTGATGCT TTGGGCCGAG
  721 GACTGCCCCG AAGTCCGGCA CCTCGTGCAC GCGGATTTCG GCTCCAACAA TGTCCTGACG
  781 GACAATGGCC GCATAACAGC GGTCATTGAC TGGAGCGAGG CGATGTTCGG GGATTCCCAA
  841 TACGAGGTCG CCAACATCTT CTTCTGGAGG CCGTGGTTGG CTTGTATGGA GCAGCAGACG
  901 CGCTACTTCG AGCGGAGGCA TCCGGAGCTT GCAGGATCGC CGCGGCTCCG GGGCGTATAT
  961 GCTCCGCATT GGGTCTTGAC CAAACTCTAT CAGAGCTTGA TTTGACGGGC AATTTCGATG
 1021 ATGCAGCCTT TGGCCGCAAG GGATTCGAAT GCGACGGCAA TCGATCCGAA TCTGGAGCTG
1081 GACTGTCGGC CGTACACCCA AAATTCG
//
Primer: reverse RDB16813_A8Lpb.seq
>D06157A9_A8LP_1_reverse_B05_05_ABI24.ab1
    1 CGCCCCAATT ACCTCACTAA AGGGAACAAA AGCTGGAGCT CCACCGCGGT GGCGGCCGCT
   61 CTAGAACTAG TGGATCCGGC GCCTGCACCA GCGCCCTTGT CATCGTCATC CTTGTAGTCG
  121 ATGTCATGAT CTTTATAATC ACCGTCATGG TCTTTGTAGT CAGCCCGGGA TCGGCTGTCC
  181 ATGTGCTGGC GTTCGAATTT TGCAGCGGCG GTTTCCTTAG ATCTAGGTCC AGGGTTCTCC
  241 TCCACGTCTC CAGCCTGCTT CAGCAGGCTG AAGTTAGTAG CTCCGCTTCC TGATTCCTTT
  301 GCCCTCGGAC GAGTGCTGGG GCGTCGGTTT CCACTATCGG CGAGTACTTC TACACAGCCA
  361 TCGGTCCAGA CGGCCGCGCT TCTGCGGGCG ATTTGTGTAC GCCCGACAGT CCCGGCTCCG
  421 GATCGGACGA TTGCGTCGCA TCGACCCTGC GCCCAAGCTG CATCATCGAA ATTGCCGTCA
  481 ACCAAGCTCT GATAGAGTTG GTCAAGACCA ATGCGGAGCA TATACGCCCG GAGCCGCGGC
  541 GATCCTGCAA GCTCCGGATG CCTCCGCTCG AAGTAGCGCG TCTGCTGCTC CATACAAGCC
  601 AACCACGGCC TCCAGAAGAA GATGTTGGCG ACCTCGTATT GGGAATCCCC GAACATCGCC
  661 TCGCTCCAGT CAATGACCGC TGTTATGCGG CCATTGTCCG TCAGGACATT GTTGGAGCCG
  721 AAATCCGCGT GCACGAGGTG CCGGACTTCG GGGCAGTCCT CGGCCCAAAG CATCAGCTCA
  781 TCGAGAGCCT GCGCGACGGA CGCACTGACG GTGTCGTCCA TCACAGTTTG CCAGTGATAC
  841 ACATGGGGGA TCAGCAATCG CGCATATGAA ATCACGCCAT GTAGTGTATT GACCGATTCC
  901 TTGCGGTCCG AATGGGCCGA ACCCGCTCGT CTGGCTAAGA TCGGCCGCAG CGATCGCATC
  961 CATGGGCCTC CGCGACCGGC TGCAGAAACA GCGGGCAGTT CGGGTTTCAG CAAGTCTTGC
 1021 AAACGTGACA CCCCTGTGCA CGGCCGGAGG ATGGCAATAG TCCAGGCTCC TCGCTGGTAT
1081 TCCCTCAATG TCAAGCCAAC CTTTTTCG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

2019.07.12

GNP_filter3_RDBDEP_html_190431.pl