Sequencing and PCR primers for primer information.
A plasmid for construction of N-terminal Hygro-P2A-Stag-3FLAG tagging donors.
| Catalog number | RDB16813 |
|---|---|
| Resource name | pMK375 (Hygro-P2A-Stag-3FLAG) |
| Clone info. | A plasmid for construction of N-terminal Hygro-P2A-Stag-3FLAG tagging donors. |
| Vector backbone | pBluescript (plasmid) |
| Size of vector backbone | 2.9 kb |
| Selectable markers | Am^r (E. coli) |
| Depositor | Kanemaki, Masato | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB16813 | pMK375 (Hygro-P2A-Stag-3FLAG) | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
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| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB16813_A8Lpp1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pUC-M13_-100 | RDB16813_A8Lpa.seq |
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>D06157A9_A8LP_1_pUC-M13_-100_A05_02_ABI24.ab1 1 GGATGTTCAA GGCGAATTAG GTTGGGTACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA 61 ACGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGG TACCGGGCCC 121 CCCCTCGAGG TCGACGGTAT CGATAAGCTT GATATCGCCA CCATGAAAAA GCCTGAACTC 181 ACCGCGACGT CTGTCGAGAA GTTTCTGATC GAAAAGTTCG ACAGCGTCTC CGACCTGATG 241 CAGCTCTCGG AGGGCGAAGA ATCTCGTGCT TTCAGCTTCG ATGTAGGAGG GCGTGGATAT 301 GTCCTGCGGG TAAATAGCTG CGCCGATGGT TTCTACAAAG ATCGTTATGT TTATCGGCAC 361 TTTGCATCGG CCGCGCTCCC GATTCCGGAA GTGCTTGACA TTGGGGAATT CAGCGAGAGC 421 CTGACCTATT GCATCTCCCG CCGTGCACAG GGTGTCACGT TGCAAGACCT GCCTGAAACC 481 GAACTGCCCG CTGTTCTGCA GCCGGTCGCG GAGGCCATGG ATGCGATCGC TGCGGCCGAT 541 CTTAGCCAGA CGAGCGGGTT CGGCCCATTC GGACCGCAAG GAATCGGTCA ATACACTACA 601 TGGCGTGATT TCATATGCGC GATTGCTGAT CCCCATGTGT ATCACTGGCA AACTGTGATG 661 GACGACACCG TCAGTGCGTC CGTCGCGCAG GCTCTCGATG AGCTGATGCT TTGGGCCGAG 721 GACTGCCCCG AAGTCCGGCA CCTCGTGCAC GCGGATTTCG GCTCCAACAA TGTCCTGACG 781 GACAATGGCC GCATAACAGC GGTCATTGAC TGGAGCGAGG CGATGTTCGG GGATTCCCAA 841 TACGAGGTCG CCAACATCTT CTTCTGGAGG CCGTGGTTGG CTTGTATGGA GCAGCAGACG 901 CGCTACTTCG AGCGGAGGCA TCCGGAGCTT GCAGGATCGC CGCGGCTCCG GGGCGTATAT 961 GCTCCGCATT GGGTCTTGAC CAAACTCTAT CAGAGCTTGA TTTGACGGGC AATTTCGATG 1021 ATGCAGCCTT TGGCCGCAAG GGATTCGAAT GCGACGGCAA TCGATCCGAA TCTGGAGCTG 1081 GACTGTCGGC CGTACACCCA AAATTCG // |
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| Primer: reverse | RDB16813_A8Lpb.seq |
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>D06157A9_A8LP_1_reverse_B05_05_ABI24.ab1 1 CGCCCCAATT ACCTCACTAA AGGGAACAAA AGCTGGAGCT CCACCGCGGT GGCGGCCGCT 61 CTAGAACTAG TGGATCCGGC GCCTGCACCA GCGCCCTTGT CATCGTCATC CTTGTAGTCG 121 ATGTCATGAT CTTTATAATC ACCGTCATGG TCTTTGTAGT CAGCCCGGGA TCGGCTGTCC 181 ATGTGCTGGC GTTCGAATTT TGCAGCGGCG GTTTCCTTAG ATCTAGGTCC AGGGTTCTCC 241 TCCACGTCTC CAGCCTGCTT CAGCAGGCTG AAGTTAGTAG CTCCGCTTCC TGATTCCTTT 301 GCCCTCGGAC GAGTGCTGGG GCGTCGGTTT CCACTATCGG CGAGTACTTC TACACAGCCA 361 TCGGTCCAGA CGGCCGCGCT TCTGCGGGCG ATTTGTGTAC GCCCGACAGT CCCGGCTCCG 421 GATCGGACGA TTGCGTCGCA TCGACCCTGC GCCCAAGCTG CATCATCGAA ATTGCCGTCA 481 ACCAAGCTCT GATAGAGTTG GTCAAGACCA ATGCGGAGCA TATACGCCCG GAGCCGCGGC 541 GATCCTGCAA GCTCCGGATG CCTCCGCTCG AAGTAGCGCG TCTGCTGCTC CATACAAGCC 601 AACCACGGCC TCCAGAAGAA GATGTTGGCG ACCTCGTATT GGGAATCCCC GAACATCGCC 661 TCGCTCCAGT CAATGACCGC TGTTATGCGG CCATTGTCCG TCAGGACATT GTTGGAGCCG 721 AAATCCGCGT GCACGAGGTG CCGGACTTCG GGGCAGTCCT CGGCCCAAAG CATCAGCTCA 781 TCGAGAGCCT GCGCGACGGA CGCACTGACG GTGTCGTCCA TCACAGTTTG CCAGTGATAC 841 ACATGGGGGA TCAGCAATCG CGCATATGAA ATCACGCCAT GTAGTGTATT GACCGATTCC 901 TTGCGGTCCG AATGGGCCGA ACCCGCTCGT CTGGCTAAGA TCGGCCGCAG CGATCGCATC 961 CATGGGCCTC CGCGACCGGC TGCAGAAACA GCGGGCAGTT CGGGTTTCAG CAAGTCTTGC 1021 AAACGTGACA CCCCTGTGCA CGGCCGGAGG ATGGCAATAG TCCAGGCTCC TCGCTGGTAT 1081 TCCCTCAATG TCAAGCCAAC CTTTTTCG // |
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Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2019.07.12
GNP_filter3_RDBDEP_html_190431.pl