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pMK375 (Hygro-P2A-Stag-3FLAG) (#RDB16813)

A plasmid for construction of N-terminal Hygro-P2A-Stag-3FLAG tagging donors.

Clone info. A plasmid for construction of N-terminal Hygro-P2A-Stag-3FLAG tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Growth conditions LB+Amp, 37oC
Depositor|Developer Kanemaki, Masato |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。

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RDB16813 pMK375 (Hygro-P2A-Stag-3FLAG) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK375 (Hygro-P2A-Stag-3FLAG) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16813).

Reference section:

Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet Data Sheet open check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 ()
Sequence file: RDB16813_A8Lpa.seq check
>D06157A9_A8LP_1_pUC-M13_-100_A05_02_ABI24.ab1
    1 GGATGTTCAA GGCGAATTAG GTTGGGTACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA
   61 ACGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGG TACCGGGCCC
  121 CCCCTCGAGG TCGACGGTAT CGATAAGCTT GATATCGCCA CCATGAAAAA GCCTGAACTC
  181 ACCGCGACGT CTGTCGAGAA GTTTCTGATC GAAAAGTTCG ACAGCGTCTC CGACCTGATG
  241 CAGCTCTCGG AGGGCGAAGA ATCTCGTGCT TTCAGCTTCG ATGTAGGAGG GCGTGGATAT
  301 GTCCTGCGGG TAAATAGCTG CGCCGATGGT TTCTACAAAG ATCGTTATGT TTATCGGCAC
  361 TTTGCATCGG CCGCGCTCCC GATTCCGGAA GTGCTTGACA TTGGGGAATT CAGCGAGAGC
  421 CTGACCTATT GCATCTCCCG CCGTGCACAG GGTGTCACGT TGCAAGACCT GCCTGAAACC
  481 GAACTGCCCG CTGTTCTGCA GCCGGTCGCG GAGGCCATGG ATGCGATCGC TGCGGCCGAT
  541 CTTAGCCAGA CGAGCGGGTT CGGCCCATTC GGACCGCAAG GAATCGGTCA ATACACTACA
  601 TGGCGTGATT TCATATGCGC GATTGCTGAT CCCCATGTGT ATCACTGGCA AACTGTGATG
  661 GACGACACCG TCAGTGCGTC CGTCGCGCAG GCTCTCGATG AGCTGATGCT TTGGGCCGAG
  721 GACTGCCCCG AAGTCCGGCA CCTCGTGCAC GCGGATTTCG GCTCCAACAA TGTCCTGACG
  781 GACAATGGCC GCATAACAGC GGTCATTGAC TGGAGCGAGG CGATGTTCGG GGATTCCCAA
  841 TACGAGGTCG CCAACATCTT CTTCTGGAGG CCGTGGTTGG CTTGTATGGA GCAGCAGACG
  901 CGCTACTTCG AGCGGAGGCA TCCGGAGCTT GCAGGATCGC CGCGGCTCCG GGGCGTATAT
  961 GCTCCGCATT GGGTCTTGAC CAAACTCTAT CAGAGCTTGA TTTGACGGGC AATTTCGATG
 1021 ATGCAGCCTT TGGCCGCAAG GGATTCGAAT GCGACGGCAA TCGATCCGAA TCTGGAGCTG
1081 GACTGTCGGC CGTACACCCA AAATTCG
//
Primer: reverse ()
Sequence file: RDB16813_A8Lpb.seq check
>D06157A9_A8LP_1_reverse_B05_05_ABI24.ab1
    1 CGCCCCAATT ACCTCACTAA AGGGAACAAA AGCTGGAGCT CCACCGCGGT GGCGGCCGCT
   61 CTAGAACTAG TGGATCCGGC GCCTGCACCA GCGCCCTTGT CATCGTCATC CTTGTAGTCG
  121 ATGTCATGAT CTTTATAATC ACCGTCATGG TCTTTGTAGT CAGCCCGGGA TCGGCTGTCC
  181 ATGTGCTGGC GTTCGAATTT TGCAGCGGCG GTTTCCTTAG ATCTAGGTCC AGGGTTCTCC
  241 TCCACGTCTC CAGCCTGCTT CAGCAGGCTG AAGTTAGTAG CTCCGCTTCC TGATTCCTTT
  301 GCCCTCGGAC GAGTGCTGGG GCGTCGGTTT CCACTATCGG CGAGTACTTC TACACAGCCA
  361 TCGGTCCAGA CGGCCGCGCT TCTGCGGGCG ATTTGTGTAC GCCCGACAGT CCCGGCTCCG
  421 GATCGGACGA TTGCGTCGCA TCGACCCTGC GCCCAAGCTG CATCATCGAA ATTGCCGTCA
  481 ACCAAGCTCT GATAGAGTTG GTCAAGACCA ATGCGGAGCA TATACGCCCG GAGCCGCGGC
  541 GATCCTGCAA GCTCCGGATG CCTCCGCTCG AAGTAGCGCG TCTGCTGCTC CATACAAGCC
  601 AACCACGGCC TCCAGAAGAA GATGTTGGCG ACCTCGTATT GGGAATCCCC GAACATCGCC
  661 TCGCTCCAGT CAATGACCGC TGTTATGCGG CCATTGTCCG TCAGGACATT GTTGGAGCCG
  721 AAATCCGCGT GCACGAGGTG CCGGACTTCG GGGCAGTCCT CGGCCCAAAG CATCAGCTCA
  781 TCGAGAGCCT GCGCGACGGA CGCACTGACG GTGTCGTCCA TCACAGTTTG CCAGTGATAC
  841 ACATGGGGGA TCAGCAATCG CGCATATGAA ATCACGCCAT GTAGTGTATT GACCGATTCC
  901 TTGCGGTCCG AATGGGCCGA ACCCGCTCGT CTGGCTAAGA TCGGCCGCAG CGATCGCATC
  961 CATGGGCCTC CGCGACCGGC TGCAGAAACA GCGGGCAGTT CGGGTTTCAG CAAGTCTTGC
 1021 AAACGTGACA CCCCTGTGCA CGGCCGGAGG ATGGCAATAG TCCAGGCTCC TCGCTGGTAT
1081 TCCCTCAATG TCAAGCCAAC CTTTTTCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [PubMed] [Article] [RRC of NBRP]