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icon below as well as clone information before placing your order.
A plasmid for construction of N-terminal Hygro-P2A-Stag-3FLAG tagging donors.
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External Database Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
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| Ordering | Order form [Credit Card Payment ] [Bank Transfer Payment]MTA, for use for not-for-profit academic purpose [Word ]MTA, for use for for-profit purpose [Word ]Please visit Information of Request for Distribution.[link] |
[open/close]| 提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 |
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| 提供依頼 | 提供依頼書 [Word ]提供同意書 (MTA, 非営利学術目的用)[Word ]提供同意書 (MTA, 営利目的用)[Word ]手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB16813 | pMK375 (Hygro-P2A-Stag-3FLAG) | DNA solution |
icon below as well as clone information before placing your order.Materials & Methods section:
| The pMK375 (Hygro-P2A-Stag-3FLAG) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16813). |
Reference section:
| Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
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| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB16813_A8Lpp1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: pUC-M13_-100 () Sequence file: RDB16813_A8Lpa.seq
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>D06157A9_A8LP_1_pUC-M13_-100_A05_02_ABI24.ab1 1 GGATGTTCAA GGCGAATTAG GTTGGGTACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA 61 ACGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGG TACCGGGCCC 121 CCCCTCGAGG TCGACGGTAT CGATAAGCTT GATATCGCCA CCATGAAAAA GCCTGAACTC 181 ACCGCGACGT CTGTCGAGAA GTTTCTGATC GAAAAGTTCG ACAGCGTCTC CGACCTGATG 241 CAGCTCTCGG AGGGCGAAGA ATCTCGTGCT TTCAGCTTCG ATGTAGGAGG GCGTGGATAT 301 GTCCTGCGGG TAAATAGCTG CGCCGATGGT TTCTACAAAG ATCGTTATGT TTATCGGCAC 361 TTTGCATCGG CCGCGCTCCC GATTCCGGAA GTGCTTGACA TTGGGGAATT CAGCGAGAGC 421 CTGACCTATT GCATCTCCCG CCGTGCACAG GGTGTCACGT TGCAAGACCT GCCTGAAACC 481 GAACTGCCCG CTGTTCTGCA GCCGGTCGCG GAGGCCATGG ATGCGATCGC TGCGGCCGAT 541 CTTAGCCAGA CGAGCGGGTT CGGCCCATTC GGACCGCAAG GAATCGGTCA ATACACTACA 601 TGGCGTGATT TCATATGCGC GATTGCTGAT CCCCATGTGT ATCACTGGCA AACTGTGATG 661 GACGACACCG TCAGTGCGTC CGTCGCGCAG GCTCTCGATG AGCTGATGCT TTGGGCCGAG 721 GACTGCCCCG AAGTCCGGCA CCTCGTGCAC GCGGATTTCG GCTCCAACAA TGTCCTGACG 781 GACAATGGCC GCATAACAGC GGTCATTGAC TGGAGCGAGG CGATGTTCGG GGATTCCCAA 841 TACGAGGTCG CCAACATCTT CTTCTGGAGG CCGTGGTTGG CTTGTATGGA GCAGCAGACG 901 CGCTACTTCG AGCGGAGGCA TCCGGAGCTT GCAGGATCGC CGCGGCTCCG GGGCGTATAT 961 GCTCCGCATT GGGTCTTGAC CAAACTCTAT CAGAGCTTGA TTTGACGGGC AATTTCGATG 1021 ATGCAGCCTT TGGCCGCAAG GGATTCGAAT GCGACGGCAA TCGATCCGAA TCTGGAGCTG 1081 GACTGTCGGC CGTACACCCA AAATTCG // |
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Primer: reverse () Sequence file: RDB16813_A8Lpb.seq
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>D06157A9_A8LP_1_reverse_B05_05_ABI24.ab1 1 CGCCCCAATT ACCTCACTAA AGGGAACAAA AGCTGGAGCT CCACCGCGGT GGCGGCCGCT 61 CTAGAACTAG TGGATCCGGC GCCTGCACCA GCGCCCTTGT CATCGTCATC CTTGTAGTCG 121 ATGTCATGAT CTTTATAATC ACCGTCATGG TCTTTGTAGT CAGCCCGGGA TCGGCTGTCC 181 ATGTGCTGGC GTTCGAATTT TGCAGCGGCG GTTTCCTTAG ATCTAGGTCC AGGGTTCTCC 241 TCCACGTCTC CAGCCTGCTT CAGCAGGCTG AAGTTAGTAG CTCCGCTTCC TGATTCCTTT 301 GCCCTCGGAC GAGTGCTGGG GCGTCGGTTT CCACTATCGG CGAGTACTTC TACACAGCCA 361 TCGGTCCAGA CGGCCGCGCT TCTGCGGGCG ATTTGTGTAC GCCCGACAGT CCCGGCTCCG 421 GATCGGACGA TTGCGTCGCA TCGACCCTGC GCCCAAGCTG CATCATCGAA ATTGCCGTCA 481 ACCAAGCTCT GATAGAGTTG GTCAAGACCA ATGCGGAGCA TATACGCCCG GAGCCGCGGC 541 GATCCTGCAA GCTCCGGATG CCTCCGCTCG AAGTAGCGCG TCTGCTGCTC CATACAAGCC 601 AACCACGGCC TCCAGAAGAA GATGTTGGCG ACCTCGTATT GGGAATCCCC GAACATCGCC 661 TCGCTCCAGT CAATGACCGC TGTTATGCGG CCATTGTCCG TCAGGACATT GTTGGAGCCG 721 AAATCCGCGT GCACGAGGTG CCGGACTTCG GGGCAGTCCT CGGCCCAAAG CATCAGCTCA 781 TCGAGAGCCT GCGCGACGGA CGCACTGACG GTGTCGTCCA TCACAGTTTG CCAGTGATAC 841 ACATGGGGGA TCAGCAATCG CGCATATGAA ATCACGCCAT GTAGTGTATT GACCGATTCC 901 TTGCGGTCCG AATGGGCCGA ACCCGCTCGT CTGGCTAAGA TCGGCCGCAG CGATCGCATC 961 CATGGGCCTC CGCGACCGGC TGCAGAAACA GCGGGCAGTT CGGGTTTCAG CAAGTCTTGC 1021 AAACGTGACA CCCCTGTGCA CGGCCGGAGG ATGGCAATAG TCCAGGCTCC TCGCTGGTAT 1081 TCCCTCAATG TCAAGCCAAC CTTTTTCG // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP] |
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2023.10.05
GNP_filter3_RDBDEP_html_231001.pl