Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK375 (Hygro-P2A-Stag-3FLAG)

A plasmid for construction of N-terminal Hygro-P2A-Stag-3FLAG tagging donors.

Catalog number RDB16813
Resource name pMK375 (Hygro-P2A-Stag-3FLAG)
Clone info. A plasmid for construction of N-terminal Hygro-P2A-Stag-3FLAG tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Depositor|Developer Kanemaki, Masato |
Other clones in our bank
External Database
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
            Ordering
            Order form [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Please visit Information of Request for Distribution.[link] 
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB16813 pMK375 (Hygro-P2A-Stag-3FLAG) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pMK375 (Hygro-P2A-Stag-3FLAG) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16813).

            Reference section:

            Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

            Tips

            Featured content

            Featured content Auxin Inducible Degron (AID) System (English text)
            Featured content Auxin Inducible Degron (AID) System (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB16813_A8Lpp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pUC-M13_-100 ()
            Sequence file: RDB16813_A8Lpa.seq check
            >D06157A9_A8LP_1_pUC-M13_-100_A05_02_ABI24.ab1
                1 GGATGTTCAA GGCGAATTAG GTTGGGTACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA
               61 ACGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGG TACCGGGCCC
              121 CCCCTCGAGG TCGACGGTAT CGATAAGCTT GATATCGCCA CCATGAAAAA GCCTGAACTC
              181 ACCGCGACGT CTGTCGAGAA GTTTCTGATC GAAAAGTTCG ACAGCGTCTC CGACCTGATG
              241 CAGCTCTCGG AGGGCGAAGA ATCTCGTGCT TTCAGCTTCG ATGTAGGAGG GCGTGGATAT
              301 GTCCTGCGGG TAAATAGCTG CGCCGATGGT TTCTACAAAG ATCGTTATGT TTATCGGCAC
              361 TTTGCATCGG CCGCGCTCCC GATTCCGGAA GTGCTTGACA TTGGGGAATT CAGCGAGAGC
              421 CTGACCTATT GCATCTCCCG CCGTGCACAG GGTGTCACGT TGCAAGACCT GCCTGAAACC
              481 GAACTGCCCG CTGTTCTGCA GCCGGTCGCG GAGGCCATGG ATGCGATCGC TGCGGCCGAT
              541 CTTAGCCAGA CGAGCGGGTT CGGCCCATTC GGACCGCAAG GAATCGGTCA ATACACTACA
              601 TGGCGTGATT TCATATGCGC GATTGCTGAT CCCCATGTGT ATCACTGGCA AACTGTGATG
              661 GACGACACCG TCAGTGCGTC CGTCGCGCAG GCTCTCGATG AGCTGATGCT TTGGGCCGAG
              721 GACTGCCCCG AAGTCCGGCA CCTCGTGCAC GCGGATTTCG GCTCCAACAA TGTCCTGACG
              781 GACAATGGCC GCATAACAGC GGTCATTGAC TGGAGCGAGG CGATGTTCGG GGATTCCCAA
              841 TACGAGGTCG CCAACATCTT CTTCTGGAGG CCGTGGTTGG CTTGTATGGA GCAGCAGACG
              901 CGCTACTTCG AGCGGAGGCA TCCGGAGCTT GCAGGATCGC CGCGGCTCCG GGGCGTATAT
              961 GCTCCGCATT GGGTCTTGAC CAAACTCTAT CAGAGCTTGA TTTGACGGGC AATTTCGATG
             1021 ATGCAGCCTT TGGCCGCAAG GGATTCGAAT GCGACGGCAA TCGATCCGAA TCTGGAGCTG
            1081 GACTGTCGGC CGTACACCCA AAATTCG
            //
            Primer: reverse ()
            Sequence file: RDB16813_A8Lpb.seq check
            >D06157A9_A8LP_1_reverse_B05_05_ABI24.ab1
                1 CGCCCCAATT ACCTCACTAA AGGGAACAAA AGCTGGAGCT CCACCGCGGT GGCGGCCGCT
               61 CTAGAACTAG TGGATCCGGC GCCTGCACCA GCGCCCTTGT CATCGTCATC CTTGTAGTCG
              121 ATGTCATGAT CTTTATAATC ACCGTCATGG TCTTTGTAGT CAGCCCGGGA TCGGCTGTCC
              181 ATGTGCTGGC GTTCGAATTT TGCAGCGGCG GTTTCCTTAG ATCTAGGTCC AGGGTTCTCC
              241 TCCACGTCTC CAGCCTGCTT CAGCAGGCTG AAGTTAGTAG CTCCGCTTCC TGATTCCTTT
              301 GCCCTCGGAC GAGTGCTGGG GCGTCGGTTT CCACTATCGG CGAGTACTTC TACACAGCCA
              361 TCGGTCCAGA CGGCCGCGCT TCTGCGGGCG ATTTGTGTAC GCCCGACAGT CCCGGCTCCG
              421 GATCGGACGA TTGCGTCGCA TCGACCCTGC GCCCAAGCTG CATCATCGAA ATTGCCGTCA
              481 ACCAAGCTCT GATAGAGTTG GTCAAGACCA ATGCGGAGCA TATACGCCCG GAGCCGCGGC
              541 GATCCTGCAA GCTCCGGATG CCTCCGCTCG AAGTAGCGCG TCTGCTGCTC CATACAAGCC
              601 AACCACGGCC TCCAGAAGAA GATGTTGGCG ACCTCGTATT GGGAATCCCC GAACATCGCC
              661 TCGCTCCAGT CAATGACCGC TGTTATGCGG CCATTGTCCG TCAGGACATT GTTGGAGCCG
              721 AAATCCGCGT GCACGAGGTG CCGGACTTCG GGGCAGTCCT CGGCCCAAAG CATCAGCTCA
              781 TCGAGAGCCT GCGCGACGGA CGCACTGACG GTGTCGTCCA TCACAGTTTG CCAGTGATAC
              841 ACATGGGGGA TCAGCAATCG CGCATATGAA ATCACGCCAT GTAGTGTATT GACCGATTCC
              901 TTGCGGTCCG AATGGGCCGA ACCCGCTCGT CTGGCTAAGA TCGGCCGCAG CGATCGCATC
              961 CATGGGCCTC CGCGACCGGC TGCAGAAACA GCGGGCAGTT CGGGTTTCAG CAAGTCTTGC
             1021 AAACGTGACA CCCCTGTGCA CGGCCGGAGG ATGGCAATAG TCCAGGCTCC TCGCTGGTAT
            1081 TCCCTCAATG TCAAGCCAAC CTTTTTCG
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

            2023.08.17

            GNP_filter3_RDBDEP_html_230702.pl