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pMK296

Donor plasmid to generate DHC1-mAID degron cell lines with mClover as a marker, neomycin resistance.

Catalog number RDB18348
Resource name pMK296
Alternative name DHC1-mAID Neo
Clone info. Donor plasmid to generate DHC1-mAID degron cell lines with mClover as a marker, neomycin resistance, see Fig. 4A-D and SFig. 6 of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone pBluescriptII KS- (plasmid)
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell).
Gene/insert name human DYNC1H1 genomic Arabidopsis thaliana IAA17 cDNA Aequorea victoria GFP (mClover) cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank
External Database
human DYNC1H1 Arabidopsis thaliana IAA17 Aequorea victoria GFP (mClover)
        Reference sequence
          
         
        Sequence (full) RDB18348zzk01.seq provided by the depositor

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
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        Catalog # Resource name Availability Shipping form Fee (non-profit org.)
        RDB18348 pMK296 Under QC test. Please contact us. DNA solution

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        References and tips

        Original reference

        original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Auxin Inducible Degron (AID) System (English text)
        Featured content Auxin Inducible Degron (AID) System (Japanese text)

        QC test results

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        References

        Original, user report and related articles

        original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

        2023.04.06

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