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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CTCF-C CRISPR

Expression vector of Cas9 and gRNA for a CTCF tagging donor.

Catalog number RDB18355
Resource name CTCF-C CRISPR
Clone info. Expression vector of Cas9 and gRNA for a CTCF tagging donor, see Fig. 4E of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone px330 (plasmid)
Selectable markers Ampicillin
Gene/insert name human CTCF cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
human CTCF

          Reference sequence
            
           
          Sequence (full) RDB18355zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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          Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
          Remarks
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word]
          備考
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB18355 CTCF-C CRISPR DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18355_B0Gfp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: SV40pA3element Reverse
          Region: U6 pro,sgRNA for CTCF,CBh pro
          Sequence file: RDB18355_B0Gfa.seq check
          >D07105C5_B0Gf_3_SV40pA3'element Reverse_E04_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNANGC NNGCAACGCG GCCTTTTTAN GGTTCCTGGC
             61 CTTTTNNNNN NNNNNNNCNN NCATGTGANG GCCTATTTCC CATGATNNCN NNNNNTTTGC
            121 ATATACGATA CAAGGCTGTT AGAGAGATAA TTGGAATTAA TTTGACTGTA AACACAAAGA
            181 TATTAGTACA AAATACGTGA CGTAGAAAGT AATAATTTCT TGGGTAGTTT GCAGTTTTAA
            241 AATTATGTTT TAAAATGGAC TATCATATGC TTACCGTAAC TTGAAAGTAT TTCGATTTCT
            301 TGGCTTTATA TATCTTGTGG AAAGGACGAA ACACCGAGAA GTCCTGGCGA CGCACAGTTT
            361 TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT ATCAACTTGA AAAAGTGGCA
            421 CCGAGTCGGT GCTTTTTTGT TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG
            481 TTTTTAGCGC GTGCGCCAAT TCTGCAGACA AATGGCTCTA GAGGTACCCG TTACATAACT
            541 TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAGT
            601 AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA
            661 CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG
            721 TAAATGGCCC GCCTGGCATT GTGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA
            781 GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTCNAN GTGAGCCCCA CGTTCTGCTT
            841 CACTCTNNNC ATCTCCCCCC CCCTCCCCAC CCCCNATTTT GTATTNATTT ATTTTTTAAT
          901 TATTTTGTGC ANNGATGGGG GCGGGGGGGG GGGGGGGNGN NNNNCNCNNN NNNNNNN
          //
          Primer: Cas9-N_R3
          Region: Cas9,SV40 NLS,3xFLAG,CBh pro
          Sequence file: RDB18355_B0Gfb.seq check
          >D07105C5_B0Gf_3_cas9_N_R3_B07_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGG NGCCGANGTC NNGGCCGANG CTGTACTTCT TGTCGGCTGC
             61 TGGGACTCCG TGGATACCGA CCTTCCGCTT CTTCTTTGGG GCCATCTTAT CGTCATCGTC
            121 TTTGTAATCA ATATCATGAT CCTTGTAGTC TCCGTCGTGG TCCTTATAGT CCATGGTGGC
            181 ACCGGTCCAA CCTGAAAAAA AGTGATTTCA GGCAGGTGCT CCAGGTAATT AAACATTAAT
            241 ACCCCACCAA CCAACCATCC CTTAAACCCT TACCTCTTGC TCAGCTAATT ACAGCCCGGA
            301 GGAGAAGGGC CGTCCCGCCC GCTCACCTGT GGGAGTAACG CGGTCAGTCA GAGCCGGGGC
          361 GGGCGGCGCG AGGCGGNNGC NGANCNNNNN NNNGNNNNNA NGNNNNNNNN NN
          //
          Primer: b-actin pro_F1
          Region: 3xFLAG,SV40 NLS,Cas9
          Sequence file: RDB18355_B0Gfc.seq check
          >D07105C5_B0Gf_3_b-actin pro_F1_F08_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGGNT GNNTGGNGGG GTANTAATGT TTAATTACCT GGAGCACCTG
             61 CCTGAANNNN NNTTTTTTCA GGTTGGACCG GTGCCACCAT GGACTATAAN GNCCACGACG
            121 GAGACTACAA GGATCATGAT ATTGATTACA AAGACGATGA CGATAAGATG GCCCCAAAGA
            181 AGAAGCGGAA GGTCGGTATC CACGGAGTCC CAGCAGCCGA CAAGAAGTAC AGCATCGGCC
            241 TGGACATCGG CACCAACTCT GTGGGCTGGG CCGTGATCAC CGACGAGTAC AAGGTGCCCA
            301 GCAAGAAATT CAAGGTGCTG GGCAACACCG ACCGGCACAG CATCAAGAAG AACCTGATCG
            361 GAGCCCTGCT GTTCGACAGC GGCGAAACAG CCGAGGCCAC CCGGCTGAAG AGAACCGCCA
            421 GAAGAAGATA CACCAGACGG AAGAACCGGA TCTGCTATCT GCAAGAGATC TTCAGCAACG
            481 AGATGGCCAA GGTGGACGAC AGCTTCTTCC ACAGACTGGA AGAGTCCTTC CTGGTGGAAG
            541 AGGATAAGAA GCACGAGCGG CACCCCATCT TCGGCAACAT CGTGGACGAG GTGGCCTACC
            601 ACGAGAAGTA CCCCACCATC TACCACCTGA GAAAGAAACT GGTGGACAGC ACCGACAAGG
            661 CCGACCTGCG GCTGATCTAT CTGGCCCTGG CCCACATGAT CAAGTTCCGG GGCCACTTCC
            721 TGATCGAGGG CGACCTGAAC CCCGACAACA GCGACGTGGA CAAGCTGTTC ATCCAGCTGG
            781 TGCAGANCTA CAACCAGCTG TTCGAGGAAA ACCCCATCAA CGCCAGCGGC GTGGACGCCN
            841 AGGCCATCCT GTCTGCCNGA NTGANCAAGA NCAGANNGCT GGAAAATCTG ATCGCCNAGC
            901 TGCCCNGNGA NAAGAAGANT GGCCTGNNCG GAAACCNNAT TGCCCTNANC CNGGGCCTGA
          961 NCCCNNACTT CNNNANCNNC TTCNNNNNNN NNN
          //
          Primer: bGH_rev3
          Region: nucleoplasmin NLS,Cas9
          Sequence file: RDB18355_B0Gfd.seq check
          >D07105C5_B0Gf_3_bGH_rev3_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNCNGAT GGCTGGCAAC
             61 TAGAAGGCAC AGTCGAGGCT GATNNNNNNN GCTCTAGGAA TTCTTACTTT TTCTTTTTTG
            121 CCTGGCCGGC CTTTTTCGTG GCCGCCGGCC TTTTGTCGCC TCCCAGCTGA GACAGGTCGA
            181 TCCGTGTCTC GTACAGGCCG GTGATGCTCT GGTGGATCAG GGTGGCGTCC AGCACCTCTT
            241 TGGTGCTGGT GTACCTCTTC CGGTCGATGG TGGTGTCAAA GTACTTGAAG GCGGCAGGGG
            301 CTCCCAGATT GGTCAGGGTA AACAGGTGGA TGATATTCTC GGCCTGCTCT CTGATGGGCT
            361 TATCCCGGTG CTTGTTGTAG GCGGACAGCA CTTTGTCCAG ATTAGCGTCG GCCAGGATCA
            421 CTCTCTTGGA GAACTCGCTG ATCTGCTCGA TGATCTCGTC CAGGTAGTGC TTGTGCTGTT
            481 CCACAAACAG CTGTTTCTGC TCATTATCCT CGGGGGAGCC CTTCAGCTTC TCATAGTGGC
            541 TGGCCAGGTA CAGGAAGTTC ACATATTTGG AGGGCAGGGC CAGTTCGTTT CCCTTCTGCA
            601 GTTCGCCGGC AGAGGCCAGC ATTCTCTTCC GGCCGTTTTC CAGCTCGAAC AGGGAGTACT
            661 TAGGCAGCTT GATGATCAGG TCCTTTTTCA CTTCTTTGTA GCCCTTGGCT TCCAGAAAGT
            721 CGATGGGANT CTTCTCNAAN CTGCTTCTTT CCATGATGGT GATCCCCAGC AGCTCTTTCA
            781 NNNTCTTCAG TTTCTTGNAC TTGNCCNTTT TCCACNTTNN GNCNCCACCN NNNNNNAANA
            841 GGCCNCGGTG GGGCTGTCGA NNCCGCCGTA NTTCTTANGG NNNNNNTCNN TTCNTNCTGG
            901 NGANNANCNN NNNNCTNTTC CTCTTNGGCA GGNNANANTC NTTNGCTGAA NCCNCCTGTN
          961 TGNACNTNNN NCTTTTNNNN NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

          2022.09.29

          GNP_filter3_RDBDEP_html_220812.pl