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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK409 (mAID-Nluc BSD)

A plasmid for construction of C-terminal mAID-Nluc BSD tagging donors.

Catalog number RDB16826
Resource name pMK409 (mAID-Nluc BSD)
Clone info. A plasmid for construction of C-terminal mAID-Nluc BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Oplophorus gracilirostris luciferase cDNA
Depositor|Developer Kanemaki, Masato |
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External Database
Arabidopsis thaliana AtIAA17 Oplophorus gracilirostris luciferase

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16826 pMK409 (mAID-Nluc BSD) DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16826_A8Lpp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-M13_-100
          Sequence file: RDB16826_A8Lpa.seq check
          >D06157C4_A8Lp_1_pUC-M13_-100_E09_15_ABI24.ab1
              1 GATGGTCAGG CGATTAGTTG GGTACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA
             61 CGGCCAGTGA GCGCGCGTAA TACGACTCAC TATAGGGCGA ATTGGAGCTC CCCGGGATCC
            121 GGTGCAGGCG CCAAGGAGAA GAGTGCTTGT CCTAAAGATC CAGCCAAACC TCCGGCCAAG
            181 GCACAAGTTG TGGGATGGCC ACCGGTGAGA TCATACCGGA AGAACGTGAT GGTTTCCTGC
            241 CAAAAATCAA GCGGTGGCCC GGAGGCGGCG GCGTTCGTGA AGGTATCAAT GGACGGAGCA
            301 CCGTACTTGA GGAAAATCGA TTTGAGGATG TATAAAGCTA GCATGGTCTT CACACTCGAA
            361 GATTTCGTTG GGGACTGGCG ACAGACAGCC GGCTACAACC TGGACCAAGT CCTTGAACAG
            421 GGAGGTGTGT CCAGTTTGTT TCAGAATCTC GGGGTGTCCG TAACTCCGAT CCAAAGGATT
            481 GTCCTGAGCG GTGAAAATGG GCTGAAGATC GACATCCATG TCATCATCCC GTATGAAGGT
            541 CTGAGCGGCG ACCAAATGGG CCAGATCGAA AAAATTTTTA AGGTGGTGTA CCCTGTGGAT
            601 GATCATCACT TTAAGGTGAT CCTGCACTAT GGCACACTGG TAATCGACGG GGTTACGCCG
            661 AACATGATCG ACTATTTCGG ACGGCCGTAT GAAGGCATCG CCGTGTTCGA CGGCAAAAAG
            721 ATCACTGTAA CAGGGACCCT GTGGAACGGC AACAAAATTA TCGACGAGCG CCTGATCAAC
            781 CCCGACGGCT CCCTGCTGTT CCGAGTAACC ATCAACGGAG TGACCGGCTG GCGGCTGTGC
            841 GAAACGCATT CTGGCGTAAG CGGCCGCTCG AGTCTAGATA ACTGATCATA ATCAGCCATA
            901 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CCTGAAACCT
            961 GAAACATAAA ATGAAATGCA ATTGTTGTTG TTAACTTTGG TTTATTGCAG CCTTATTAAT
           1021 TGGTTTACAA ATAAAGCCAA TTAGCATTCA CAAACTTTCA ACAAACTAAG CATTTTTTCT
          1081 CACTGCCATT CCTTAAGTTT GTTTG
          //
          Primer: reverse
          Sequence file: RDB16826_A8Lpb.seq check
          >D06157C4_A8Lp_1_reverse_F09_18_ABI24.ab1
              1 CAAAACATTA CCCTCACTAA AGGGAACAAA AGCTGGGTAC CGGATCCTAG TGAACCTCTT
             61 CGAGGGACCT AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA GGGTTCCGGA
            121 TCTCGACCAG CTTCTGATGG AATTAGAACT TGGCAAAACA ATACTGAGAA TGAAGTGTAT
            181 GTGGAACAGA GGCTGCTGAT CTCGTTCTTC AGGCTATGAA ACTGACACAT TTGGAAACCA
            241 CAGTACTTAG AACCACAAAG TGGGAATCAA GAGAAAAACA ATGATCCCAC GAGAGATCTA
            301 TAGATCTATA GATCATGAGT GGGAGGAATG AGCTGGCCCT TAATTTGGTT TTGCTTGTTT
            361 AAATTATGAT ATCCAACTAT GAAACATTAT CATAAAGCAA TAGTAAAGAG CCTTCAGTAA
            421 AGAGCAGGCA TTTATCTAAT CCCACCCCAC CCCCACCCCC GTAGCTCCAA TCCTTCCATT
            481 CAAAATGTAG GTACTCTGTT CTCACCCTTC TTAACAAAGT ATGACAGGAA AAACTTCCAT
            541 TTTAGTGGAC ATCTTTATTG TTTAATAGAT CATCAATTTC TGCATCCCGG GGATCTGATA
            601 TCATCGATTA GCCCTCCCAC ACATAACCAG AGGGCAGCAA TTCACGAATC CCAACTGCCG
            661 TCGGCTGTCC ATCACTGTCC TTCACTATGG CTTTGATCCC AGGATGCAGA TCGAGAAGCA
            721 CCTGTCGGCA CCGTCCGCAG GGGCTCAAGA TGCCCCTGTT CTCATTTCCG ATCGCGACGA
            781 TACAAGTCAG GTTGCCAGCT GCCGCAGCAG CAGCAGTGCC CAGCACCACG AGTTCTGCAC
            841 AAGGTCCCCC CAGTAAAATG ATATACATTG ACACCAGTGA AGATGCGGCC GTCGCTAGAG
            901 AGAGCTGCGC TGGCGACGCT GTAGTCTTCA GAGATGGGGG ATGCTGTTGA TTGTAGCCCG
            961 TTGCTCTTTC AATGAGGTGG ATTCTTCTTT GAGACAAAGG CTTGGCCCAT GGTGGCTCGA
           1021 GAATCTAGAT GATGGCAAGT CGAACGCCAG GAGATGAGGA GAAGGAGAAC AGCGCCGGCA
          1081 GAACGGTGC
          //
          Primer: PGK_N_rev
          Sequence file: RDB16826_A8Lpc.seq check
          >D06157C4_A8Lp_1_PGK_N_rev_G09_21_ABI24.ab1
              1 CCAGGGGCGG CCTCCCTACC CGGTAGATTT CGAGGTCGAG ATCCTAAGCT TGGCTGGACG
             61 TAAACTCCTC TTCAGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG
            121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA
            181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA
            241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG
            301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
            361 CTGATTATGA TCAGTTATCT AGACTCGAGC GGCCGCTTAC GCCAGAATGC GTTCGCACAG
            421 CCGCCAGCCG GTCACTCCGT TGATGGTTAC TCGGAACAGC AGGGAGCCGT CGGGGTTGAT
            481 CAGGCGCTCG TCGATAATTT TGTTGCCGTT CCACAGGGTC CCTGTTACAG TGATCTTTTT
            541 GCCGTCGAAC ACGGCGATGC CTTCATACGG CCGTCCGAAA TAGTCGATCA TGTTCGGCGT
            601 AACCCCGTCG ATTACCAGTG TGCCATAGTG CAGGATCACC TTAAAGTGAT GATCATCCAC
            661 AGGGTACACC ACCTTAAAAA TTTTTTCGAT CTGGCCCATT TGGTCGCCGC TCAGACCTTC
            721 ATACGGGATG ATGACATGGA TGTCGATCTT CAGCCCATTT TCACCGCTCA GGACAATCCT
            781 TTGGATCGGA GTTACGGACA CCCCGAGATT CTGAAACAAA CTGGACACAC CTCCCTGTTC
            841 AAGGACTTGG TCCAGGTTGT AGCCGGCTGT CTGTCGCCAG TCCCCAACGA AATCTTCGAG
            901 TGTGAAGACC ATGCTAGCTT TATACATCCT CAAATCGATT TTCCTCAAGT ACGGTGCTCC
            961 GTCCCATTGA TACCTTCACG AACGCCCGCC GCCTCCTGGC CACCGCTTTG ATTTTTGGCA
           1021 GAAACAATCA CGTTCTTCCG GTATGGATCT CACCGGTGGT CATCCACACT GTGCCTTGAC
          1081 TGAGTTGGGC CTGGGATC
          //
          Primer: PGKpro_F
          Sequence file: RDB16826_A8Lpd.seq check
          >D06157C4_A8Lp_1_PGKpro_F_H09_24_ABI24.ab1
              1 AGTGGCGCCT ATCTCTCTTC TCATCTCCGG GCCTTTCGAC CTGCATCCAT CTAGATCTCG
             61 AGCCACCATG GCCAAGCCTT TGTCTCAAGA AGAATCCACC CTCATTGAAA GAGCAACGGC
            121 TACAATCAAC AGCATCCCCA TCTCTGAAGA CTACAGCGTC GCCAGCGCAG CTCTCTCTAG
            181 CGACGGCCGC ATCTTCACTG GTGTCAATGT ATATCATTTT ACTGGGGGAC CTTGTGCAGA
            241 ACTCGTGGTG CTGGGCACTG CTGCTGCTGC GGCAGCTGGC AACCTGACTT GTATCGTCGC
            301 GATCGGAAAT GAGAACAGGG GCATCTTGAG CCCCTGCGGA CGGTGCCGAC AGGTGCTTCT
            361 CGATCTGCAT CCTGGGATCA AAGCCATAGT GAAGGACAGT GATGGACAGC CGACGGCAGT
            421 TGGGATTCGT GAATTGCTGC CCTCTGGTTA TGTGTGGGAG GGCTAATCGA TGATATCAGA
            481 TCCCCGGGAT GCAGAAATTG ATGATCTATT AAACAATAAA GATGTCCACT AAAATGGAAG
            541 TTTTTCCTGT CATACTTTGT TAAGAAGGGT GAGAACAGAG TACCTACATT TTGAATGGAA
            601 GGATTGGAGC TACGGGGGTG GGGGTGGGGT GGGATTAGAT AAATGCCTGC TCTTTACTGA
            661 AGGCTCTTTA CTATTGCTTT ATGATAATGT TTCATAGTTG GATATCATAA TTTAAACAAG
          721 CAAAACCAAA TTAAGGGCCA GCTCATTCCT CCCACT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          2021.11.02

          GNP_filter3_RDBDEP_html_211029.pl