Resource data sheet
Prev.

pMK409 (mAID-Nluc BSD)

A plasmid for construction of C-terminal mAID-Nluc BSD tagging donors.

Catalog number RDB16826
Resource name pMK409 (mAID-Nluc BSD)
Clone info. A plasmid for construction of C-terminal mAID-Nluc BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Oplophorus gracilirostris luciferase cDNA
Depositor Kanemaki, Masato |
 
Other clones in our bank Arabidopsis thaliana AXR3 (KEGG orthology K14484) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16826 pMK409 (mAID-Nluc BSD) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16826_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 RDB16826_A8Lpa.seq
>D06157C4_A8Lp_1_pUC-M13_-100_E09_15_ABI24.ab1
    1 GATGGTCAGG CGATTAGTTG GGTACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA
   61 CGGCCAGTGA GCGCGCGTAA TACGACTCAC TATAGGGCGA ATTGGAGCTC CCCGGGATCC
  121 GGTGCAGGCG CCAAGGAGAA GAGTGCTTGT CCTAAAGATC CAGCCAAACC TCCGGCCAAG
  181 GCACAAGTTG TGGGATGGCC ACCGGTGAGA TCATACCGGA AGAACGTGAT GGTTTCCTGC
  241 CAAAAATCAA GCGGTGGCCC GGAGGCGGCG GCGTTCGTGA AGGTATCAAT GGACGGAGCA
  301 CCGTACTTGA GGAAAATCGA TTTGAGGATG TATAAAGCTA GCATGGTCTT CACACTCGAA
  361 GATTTCGTTG GGGACTGGCG ACAGACAGCC GGCTACAACC TGGACCAAGT CCTTGAACAG
  421 GGAGGTGTGT CCAGTTTGTT TCAGAATCTC GGGGTGTCCG TAACTCCGAT CCAAAGGATT
  481 GTCCTGAGCG GTGAAAATGG GCTGAAGATC GACATCCATG TCATCATCCC GTATGAAGGT
  541 CTGAGCGGCG ACCAAATGGG CCAGATCGAA AAAATTTTTA AGGTGGTGTA CCCTGTGGAT
  601 GATCATCACT TTAAGGTGAT CCTGCACTAT GGCACACTGG TAATCGACGG GGTTACGCCG
  661 AACATGATCG ACTATTTCGG ACGGCCGTAT GAAGGCATCG CCGTGTTCGA CGGCAAAAAG
  721 ATCACTGTAA CAGGGACCCT GTGGAACGGC AACAAAATTA TCGACGAGCG CCTGATCAAC
  781 CCCGACGGCT CCCTGCTGTT CCGAGTAACC ATCAACGGAG TGACCGGCTG GCGGCTGTGC
  841 GAAACGCATT CTGGCGTAAG CGGCCGCTCG AGTCTAGATA ACTGATCATA ATCAGCCATA
  901 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CCTGAAACCT
  961 GAAACATAAA ATGAAATGCA ATTGTTGTTG TTAACTTTGG TTTATTGCAG CCTTATTAAT
 1021 TGGTTTACAA ATAAAGCCAA TTAGCATTCA CAAACTTTCA ACAAACTAAG CATTTTTTCT
1081 CACTGCCATT CCTTAAGTTT GTTTG
//
Primer: reverse RDB16826_A8Lpb.seq
>D06157C4_A8Lp_1_reverse_F09_18_ABI24.ab1
    1 CAAAACATTA CCCTCACTAA AGGGAACAAA AGCTGGGTAC CGGATCCTAG TGAACCTCTT
   61 CGAGGGACCT AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA GGGTTCCGGA
  121 TCTCGACCAG CTTCTGATGG AATTAGAACT TGGCAAAACA ATACTGAGAA TGAAGTGTAT
  181 GTGGAACAGA GGCTGCTGAT CTCGTTCTTC AGGCTATGAA ACTGACACAT TTGGAAACCA
  241 CAGTACTTAG AACCACAAAG TGGGAATCAA GAGAAAAACA ATGATCCCAC GAGAGATCTA
  301 TAGATCTATA GATCATGAGT GGGAGGAATG AGCTGGCCCT TAATTTGGTT TTGCTTGTTT
  361 AAATTATGAT ATCCAACTAT GAAACATTAT CATAAAGCAA TAGTAAAGAG CCTTCAGTAA
  421 AGAGCAGGCA TTTATCTAAT CCCACCCCAC CCCCACCCCC GTAGCTCCAA TCCTTCCATT
  481 CAAAATGTAG GTACTCTGTT CTCACCCTTC TTAACAAAGT ATGACAGGAA AAACTTCCAT
  541 TTTAGTGGAC ATCTTTATTG TTTAATAGAT CATCAATTTC TGCATCCCGG GGATCTGATA
  601 TCATCGATTA GCCCTCCCAC ACATAACCAG AGGGCAGCAA TTCACGAATC CCAACTGCCG
  661 TCGGCTGTCC ATCACTGTCC TTCACTATGG CTTTGATCCC AGGATGCAGA TCGAGAAGCA
  721 CCTGTCGGCA CCGTCCGCAG GGGCTCAAGA TGCCCCTGTT CTCATTTCCG ATCGCGACGA
  781 TACAAGTCAG GTTGCCAGCT GCCGCAGCAG CAGCAGTGCC CAGCACCACG AGTTCTGCAC
  841 AAGGTCCCCC CAGTAAAATG ATATACATTG ACACCAGTGA AGATGCGGCC GTCGCTAGAG
  901 AGAGCTGCGC TGGCGACGCT GTAGTCTTCA GAGATGGGGG ATGCTGTTGA TTGTAGCCCG
  961 TTGCTCTTTC AATGAGGTGG ATTCTTCTTT GAGACAAAGG CTTGGCCCAT GGTGGCTCGA
 1021 GAATCTAGAT GATGGCAAGT CGAACGCCAG GAGATGAGGA GAAGGAGAAC AGCGCCGGCA
1081 GAACGGTGC
//
Primer: PGK_N_rev RDB16826_A8Lpc.seq
>D06157C4_A8Lp_1_PGK_N_rev_G09_21_ABI24.ab1
    1 CCAGGGGCGG CCTCCCTACC CGGTAGATTT CGAGGTCGAG ATCCTAAGCT TGGCTGGACG
   61 TAAACTCCTC TTCAGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG
  121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA
  181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA
  241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG
  301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
  361 CTGATTATGA TCAGTTATCT AGACTCGAGC GGCCGCTTAC GCCAGAATGC GTTCGCACAG
  421 CCGCCAGCCG GTCACTCCGT TGATGGTTAC TCGGAACAGC AGGGAGCCGT CGGGGTTGAT
  481 CAGGCGCTCG TCGATAATTT TGTTGCCGTT CCACAGGGTC CCTGTTACAG TGATCTTTTT
  541 GCCGTCGAAC ACGGCGATGC CTTCATACGG CCGTCCGAAA TAGTCGATCA TGTTCGGCGT
  601 AACCCCGTCG ATTACCAGTG TGCCATAGTG CAGGATCACC TTAAAGTGAT GATCATCCAC
  661 AGGGTACACC ACCTTAAAAA TTTTTTCGAT CTGGCCCATT TGGTCGCCGC TCAGACCTTC
  721 ATACGGGATG ATGACATGGA TGTCGATCTT CAGCCCATTT TCACCGCTCA GGACAATCCT
  781 TTGGATCGGA GTTACGGACA CCCCGAGATT CTGAAACAAA CTGGACACAC CTCCCTGTTC
  841 AAGGACTTGG TCCAGGTTGT AGCCGGCTGT CTGTCGCCAG TCCCCAACGA AATCTTCGAG
  901 TGTGAAGACC ATGCTAGCTT TATACATCCT CAAATCGATT TTCCTCAAGT ACGGTGCTCC
  961 GTCCCATTGA TACCTTCACG AACGCCCGCC GCCTCCTGGC CACCGCTTTG ATTTTTGGCA
 1021 GAAACAATCA CGTTCTTCCG GTATGGATCT CACCGGTGGT CATCCACACT GTGCCTTGAC
1081 TGAGTTGGGC CTGGGATC
//
Primer: PGKpro_F RDB16826_A8Lpd.seq
>D06157C4_A8Lp_1_PGKpro_F_H09_24_ABI24.ab1
    1 AGTGGCGCCT ATCTCTCTTC TCATCTCCGG GCCTTTCGAC CTGCATCCAT CTAGATCTCG
   61 AGCCACCATG GCCAAGCCTT TGTCTCAAGA AGAATCCACC CTCATTGAAA GAGCAACGGC
  121 TACAATCAAC AGCATCCCCA TCTCTGAAGA CTACAGCGTC GCCAGCGCAG CTCTCTCTAG
  181 CGACGGCCGC ATCTTCACTG GTGTCAATGT ATATCATTTT ACTGGGGGAC CTTGTGCAGA
  241 ACTCGTGGTG CTGGGCACTG CTGCTGCTGC GGCAGCTGGC AACCTGACTT GTATCGTCGC
  301 GATCGGAAAT GAGAACAGGG GCATCTTGAG CCCCTGCGGA CGGTGCCGAC AGGTGCTTCT
  361 CGATCTGCAT CCTGGGATCA AAGCCATAGT GAAGGACAGT GATGGACAGC CGACGGCAGT
  421 TGGGATTCGT GAATTGCTGC CCTCTGGTTA TGTGTGGGAG GGCTAATCGA TGATATCAGA
  481 TCCCCGGGAT GCAGAAATTG ATGATCTATT AAACAATAAA GATGTCCACT AAAATGGAAG
  541 TTTTTCCTGT CATACTTTGT TAAGAAGGGT GAGAACAGAG TACCTACATT TTGAATGGAA
  601 GGATTGGAGC TACGGGGGTG GGGGTGGGGT GGGATTAGAT AAATGCCTGC TCTTTACTGA
  661 AGGCTCTTTA CTATTGCTTT ATGATAATGT TTCATAGTTG GATATCATAA TTTAAACAAG
721 CAAAACCAAA TTAAGGGCCA GCTCATTCCT CCCACT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

2019.07.12

GNP_filter3_RDBDEP_html_190431.pl