RDB16826 - pMK409 (mAID-Nluc BSD)

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pMK409 (mAID-Nluc BSD) (#RDB16826)

A plasmid for construction of C-terminal mAID-Nluc BSD tagging donors.

Clone info.	A plasmid for construction of C-terminal mAID-Nluc BSD tagging donors.
Vector backbone	pBluescript (plasmid)
Size of vector backbone	2.9 kb
Selectable markers	Am^r (E. coli)
Gene/insert name	Arabidopsis thaliana AtIAA17 cDNA Oplophorus gracilirostris luciferase cDNA
Depositor\|Developer	Kanemaki, Masato \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB16826	pMK409 (mAID-Nluc BSD)	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK409 (mAID-Nluc BSD) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16826).

Reference section:

Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB16826_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 () Sequence file: RDB16826_A8Lpa.seq
>D06157C4_A8Lp_1_pUC-M13_-100_E09_15_ABI24.ab1 1 GATGGTCAGG CGATTAGTTG GGTACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA 61 CGGCCAGTGA GCGCGCGTAA TACGACTCAC TATAGGGCGA ATTGGAGCTC CCCGGGATCC 121 GGTGCAGGCG CCAAGGAGAA GAGTGCTTGT CCTAAAGATC CAGCCAAACC TCCGGCCAAG 181 GCACAAGTTG TGGGATGGCC ACCGGTGAGA TCATACCGGA AGAACGTGAT GGTTTCCTGC 241 CAAAAATCAA GCGGTGGCCC GGAGGCGGCG GCGTTCGTGA AGGTATCAAT GGACGGAGCA 301 CCGTACTTGA GGAAAATCGA TTTGAGGATG TATAAAGCTA GCATGGTCTT CACACTCGAA 361 GATTTCGTTG GGGACTGGCG ACAGACAGCC GGCTACAACC TGGACCAAGT CCTTGAACAG 421 GGAGGTGTGT CCAGTTTGTT TCAGAATCTC GGGGTGTCCG TAACTCCGAT CCAAAGGATT 481 GTCCTGAGCG GTGAAAATGG GCTGAAGATC GACATCCATG TCATCATCCC GTATGAAGGT 541 CTGAGCGGCG ACCAAATGGG CCAGATCGAA AAAATTTTTA AGGTGGTGTA CCCTGTGGAT 601 GATCATCACT TTAAGGTGAT CCTGCACTAT GGCACACTGG TAATCGACGG GGTTACGCCG 661 AACATGATCG ACTATTTCGG ACGGCCGTAT GAAGGCATCG CCGTGTTCGA CGGCAAAAAG 721 ATCACTGTAA CAGGGACCCT GTGGAACGGC AACAAAATTA TCGACGAGCG CCTGATCAAC 781 CCCGACGGCT CCCTGCTGTT CCGAGTAACC ATCAACGGAG TGACCGGCTG GCGGCTGTGC 841 GAAACGCATT CTGGCGTAAG CGGCCGCTCG AGTCTAGATA ACTGATCATA ATCAGCCATA 901 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CCTGAAACCT 961 GAAACATAAA ATGAAATGCA ATTGTTGTTG TTAACTTTGG TTTATTGCAG CCTTATTAAT 1021 TGGTTTACAA ATAAAGCCAA TTAGCATTCA CAAACTTTCA ACAAACTAAG CATTTTTTCT 1081 CACTGCCATT CCTTAAGTTT GTTTG //
Primer: reverse () Sequence file: RDB16826_A8Lpb.seq
>D06157C4_A8Lp_1_reverse_F09_18_ABI24.ab1 1 CAAAACATTA CCCTCACTAA AGGGAACAAA AGCTGGGTAC CGGATCCTAG TGAACCTCTT 61 CGAGGGACCT AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA GGGTTCCGGA 121 TCTCGACCAG CTTCTGATGG AATTAGAACT TGGCAAAACA ATACTGAGAA TGAAGTGTAT 181 GTGGAACAGA GGCTGCTGAT CTCGTTCTTC AGGCTATGAA ACTGACACAT TTGGAAACCA 241 CAGTACTTAG AACCACAAAG TGGGAATCAA GAGAAAAACA ATGATCCCAC GAGAGATCTA 301 TAGATCTATA GATCATGAGT GGGAGGAATG AGCTGGCCCT TAATTTGGTT TTGCTTGTTT 361 AAATTATGAT ATCCAACTAT GAAACATTAT CATAAAGCAA TAGTAAAGAG CCTTCAGTAA 421 AGAGCAGGCA TTTATCTAAT CCCACCCCAC CCCCACCCCC GTAGCTCCAA TCCTTCCATT 481 CAAAATGTAG GTACTCTGTT CTCACCCTTC TTAACAAAGT ATGACAGGAA AAACTTCCAT 541 TTTAGTGGAC ATCTTTATTG TTTAATAGAT CATCAATTTC TGCATCCCGG GGATCTGATA 601 TCATCGATTA GCCCTCCCAC ACATAACCAG AGGGCAGCAA TTCACGAATC CCAACTGCCG 661 TCGGCTGTCC ATCACTGTCC TTCACTATGG CTTTGATCCC AGGATGCAGA TCGAGAAGCA 721 CCTGTCGGCA CCGTCCGCAG GGGCTCAAGA TGCCCCTGTT CTCATTTCCG ATCGCGACGA 781 TACAAGTCAG GTTGCCAGCT GCCGCAGCAG CAGCAGTGCC CAGCACCACG AGTTCTGCAC 841 AAGGTCCCCC CAGTAAAATG ATATACATTG ACACCAGTGA AGATGCGGCC GTCGCTAGAG 901 AGAGCTGCGC TGGCGACGCT GTAGTCTTCA GAGATGGGGG ATGCTGTTGA TTGTAGCCCG 961 TTGCTCTTTC AATGAGGTGG ATTCTTCTTT GAGACAAAGG CTTGGCCCAT GGTGGCTCGA 1021 GAATCTAGAT GATGGCAAGT CGAACGCCAG GAGATGAGGA GAAGGAGAAC AGCGCCGGCA 1081 GAACGGTGC //
Primer: PGK_N_rev () Sequence file: RDB16826_A8Lpc.seq
>D06157C4_A8Lp_1_PGK_N_rev_G09_21_ABI24.ab1 1 CCAGGGGCGG CCTCCCTACC CGGTAGATTT CGAGGTCGAG ATCCTAAGCT TGGCTGGACG 61 TAAACTCCTC TTCAGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG 121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA 181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA 241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG 301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG 361 CTGATTATGA TCAGTTATCT AGACTCGAGC GGCCGCTTAC GCCAGAATGC GTTCGCACAG 421 CCGCCAGCCG GTCACTCCGT TGATGGTTAC TCGGAACAGC AGGGAGCCGT CGGGGTTGAT 481 CAGGCGCTCG TCGATAATTT TGTTGCCGTT CCACAGGGTC CCTGTTACAG TGATCTTTTT 541 GCCGTCGAAC ACGGCGATGC CTTCATACGG CCGTCCGAAA TAGTCGATCA TGTTCGGCGT 601 AACCCCGTCG ATTACCAGTG TGCCATAGTG CAGGATCACC TTAAAGTGAT GATCATCCAC 661 AGGGTACACC ACCTTAAAAA TTTTTTCGAT CTGGCCCATT TGGTCGCCGC TCAGACCTTC 721 ATACGGGATG ATGACATGGA TGTCGATCTT CAGCCCATTT TCACCGCTCA GGACAATCCT 781 TTGGATCGGA GTTACGGACA CCCCGAGATT CTGAAACAAA CTGGACACAC CTCCCTGTTC 841 AAGGACTTGG TCCAGGTTGT AGCCGGCTGT CTGTCGCCAG TCCCCAACGA AATCTTCGAG 901 TGTGAAGACC ATGCTAGCTT TATACATCCT CAAATCGATT TTCCTCAAGT ACGGTGCTCC 961 GTCCCATTGA TACCTTCACG AACGCCCGCC GCCTCCTGGC CACCGCTTTG ATTTTTGGCA 1021 GAAACAATCA CGTTCTTCCG GTATGGATCT CACCGGTGGT CATCCACACT GTGCCTTGAC 1081 TGAGTTGGGC CTGGGATC //
Primer: PGKpro_F () Sequence file: RDB16826_A8Lpd.seq
>D06157C4_A8Lp_1_PGKpro_F_H09_24_ABI24.ab1 1 AGTGGCGCCT ATCTCTCTTC TCATCTCCGG GCCTTTCGAC CTGCATCCAT CTAGATCTCG 61 AGCCACCATG GCCAAGCCTT TGTCTCAAGA AGAATCCACC CTCATTGAAA GAGCAACGGC 121 TACAATCAAC AGCATCCCCA TCTCTGAAGA CTACAGCGTC GCCAGCGCAG CTCTCTCTAG 181 CGACGGCCGC ATCTTCACTG GTGTCAATGT ATATCATTTT ACTGGGGGAC CTTGTGCAGA 241 ACTCGTGGTG CTGGGCACTG CTGCTGCTGC GGCAGCTGGC AACCTGACTT GTATCGTCGC 301 GATCGGAAAT GAGAACAGGG GCATCTTGAG CCCCTGCGGA CGGTGCCGAC AGGTGCTTCT 361 CGATCTGCAT CCTGGGATCA AAGCCATAGT GAAGGACAGT GATGGACAGC CGACGGCAGT 421 TGGGATTCGT GAATTGCTGC CCTCTGGTTA TGTGTGGGAG GGCTAATCGA TGATATCAGA 481 TCCCCGGGAT GCAGAAATTG ATGATCTATT AAACAATAAA GATGTCCACT AAAATGGAAG 541 TTTTTCCTGT CATACTTTGT TAAGAAGGGT GAGAACAGAG TACCTACATT TTGAATGGAA 601 GGATTGGAGC TACGGGGGTG GGGGTGGGGT GGGATTAGAT AAATGCCTGC TCTTTACTGA 661 AGGCTCTTTA CTATTGCTTT ATGATAATGT TTCATAGTTG GATATCATAA TTTAAACAAG 721 CAAAACCAAA TTAAGGGCCA GCTCATTCCT CCCACT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]