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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK344 (Hygro-P2A-mAID) (#RDB16805)

A plasmid for construction of N-terminal Hygro-P2A-mAID tagging donors.

Clone info. A plasmid for construction of N-terminal Hygro-P2A-mAID tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA
Depositor|Developer Kanemaki, Masato |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。

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RDB16805 pMK344 (Hygro-P2A-mAID) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK344 (Hygro-P2A-mAID) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16805).

Reference section:

Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16805_A8Lpp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310)
Region: T7 pro,HygR
Sequence file: RDB16805_A8Lpc.seq check
>D06157A1_A8LP_2_pUC-M13_-100_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNANN NNNNNNNNCC NNNGTTTTCC CNGTCACGAC GTTGTAAAAC
   61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGGTA CCGGGCCCCC
  121 CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGCCACC ATGAAAAAGC CTGAACTCAC
  181 CGCGACGTCT GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA
  241 GCTCTCGGAG GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT
  301 CCTGCGGGTA AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT
  361 TGCATCGGCC GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT
  421 GACCTATTGC ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA
  481 ACTGCCCGCT GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT
  541 TAGCCAGACG AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG
  601 GCGTGATTTC ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA
  661 CGACACCGTC AGTGCGTCCG TCGCGCANGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA
  721 CTGCCCCGAA GTCCGGCACC TCGTGCACGC GGATTTCNGC TCCNACAATG TCCTGACGGA
  781 CAATGGCCGC ATAACAGCGG TCATTGNCTG GANCGAGGCG ATGTTCGGGG ATTCCCAATA
  841 CGANGTCNCC AACNTCTTCN TCTGGANGNC GNGGTTGGCT TGNATGGAGC ANCANANNNN
  901 CTANTTCNNN NNGNANGCNT NCNNANNNTN NNNNNNCGNN NNCNNCNNCN NGNNNNANNN
961 NNNNNNN
//
Primer: reverse (Pr0099)
Region: Linker,mAID,P2A,HygR
Sequence file: RDB16805_A8Lpd.seq check
>D06157A1_A8LP_2_reverse_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGNANN NAAGCTGGAG CTCCNCCGCG GTGGCGGCCG
   61 CTCTAGAACT AGTGGATCCG GCGCCTGCAC CAGCGCCTTT ATACATCCTC AAATCGATTT
  121 TCCTCAAGTA CGGTGCTCCG TCCATTGATA CCTTCACGAA CGCCGCCGCC TCCGGGCCAC
  181 CGCTTGATTT TTGGCAGGAA ACCATCACGT TCTTCCGGTA TGATCTCACC GGTGGCCATC
  241 CCACAACTTG TGCCTTGGCC GGAGGTTTGG CTGGATCTTT AGGACAAGCA CTCTTCTCCT
  301 TAGATCTAGG TCCAGGGTTC TCCTCCACGT CTCCAGCCTG CTTCAGCAGG CTGAAGTTAG
  361 TAGCTCCGCT TCCTGATTCC TTTGCCCTCG GACGAGTGCT GGGGCGTCGG TTTCCACTAT
  421 CGGCGAGTAC TTCTACACAG CCATCGGTCC AGACGGCCGC GCTTCTGCGG GCGATTTGTG
  481 TACGCCCGAC AGTCCCGGCT CCGGATCGGA CGATTGCGTC GCATCGACCC TGCGCCCAAG
  541 CTGCATCATC GAAATTGCCG TCAACCAAGC TCTGATAGAG TTGGTCAAGA CCAATGCGGA
  601 GCATATACGC CCGGAGCCGC GGCGATCCTG CAAGCTCCGG ATGCCTCCGC TCGAAGTAGC
  661 GCGTCTGCTG CTCCATACAA GCCAACCACG GCCTCCAGAA GAAGATGTTG GCGANCTCGT
  721 ATTGGGAATC CCCGAACATC GCCTCGCTCC AGTCAATGAC CGCTGTTATG CGGCCATTGT
  781 CCGTCANGAC ATTGTTGGAN CCGAAATCCG CGNGCACGAN GTGCCNGANT TCNGGGCAGT
  841 CCTCGGNCNN AAGCATCNGC TCATCNNGAN NCTGCNCGAN GGANNCACTN NCGGNGNCGN
  901 NCATCNNANN TNGNNANNGA TACNCNNNGG NNNCAGNNNT CGCGCATATN NNNCNCGCCN
961 NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]