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pMK344 (Hygro-P2A-mAID)

A plasmid for construction of N-terminal Hygro-P2A-mAID tagging donors.

Catalog number RDB16805
Resource name pMK344 (Hygro-P2A-mAID)
Clone info. A plasmid for construction of N-terminal Hygro-P2A-mAID tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA
Depositor Kanemaki, Masato |
 
Other clones in our bank Arabidopsis thaliana AXR3 (KEGG orthology K14484) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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RDB16805 pMK344 (Hygro-P2A-mAID) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16805_A8Lpp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 RDB16805_A8Lpa.seq
>D06157A1_A8LP_2_pUC-M13_-100_A01_01_ABI24.ab1
    1 GGACCTGTGG GCGGATTAGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC
   61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGGTA CCGGGCCCCC
  121 CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGCCACC ATGAAAAAGC CTGAACTCAC
  181 CGCGACGTCT GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA
  241 GCTCTCGGAG GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT
  301 CCTGCGGGTA AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT
  361 TGCATCGGCC GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT
  421 GACCTATTGC ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA
  481 ACTGCCCGCT GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT
  541 TAGCCAGACG AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG
  601 GCGTGATTTC ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA
  661 CGACACCGTC AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA
  721 CTGCCCCGAA GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA
  781 CAATGGCCGC ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA
  841 CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT TGTATGGAGC AGCAGACGCG
  901 CTACTTCGAG CGGGAGGCAT CCGGAGCTTT GCAGATCGCC CGCGGCTCCC GGGCGTATAT
  961 GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTTGTT GACGGCATTT CGAATGATGC
 1021 AGCCTTGGGC GCAGGGTTCG ATGCGACGCA TTCGGTTCGA TCGAAGCTGA ACTGGTCGGG
1081 GCGTACACTA AAATTCGGTC CTGGTCA
//
Primer: reverse RDB16805_A8Lpb.seq
>D06157A1_A8LP_2_reverse_B01_04_ABI24.ab1
    1 CCCGGCAAAT TAAACCTCAC TAAAGGGACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG
   61 CTCTAGAACT AGTGGATCCG GCGCCTGCAC CAGCGCCTTT ATACATCCTC AAATCGATTT
  121 TCCTCAAGTA CGGTGCTCCG TCCATTGATA CCTTCACGAA CGCCGCCGCC TCCGGGCCAC
  181 CGCTTGATTT TTGGCAGGAA ACCATCACGT TCTTCCGGTA TGATCTCACC GGTGGCCATC
  241 CCACAACTTG TGCCTTGGCC GGAGGTTTGG CTGGATCTTT AGGACAAGCA CTCTTCTCCT
  301 TAGATCTAGG TCCAGGGTTC TCCTCCACGT CTCCAGCCTG CTTCAGCAGG CTGAAGTTAG
  361 TAGCTCCGCT TCCTGATTCC TTTGCCCTCG GACGAGTGCT GGGGCGTCGG TTTCCACTAT
  421 CGGCGAGTAC TTCTACACAG CCATCGGTCC AGACGGCCGC GCTTCTGCGG GCGATTTGTG
  481 TACGCCCGAC AGTCCCGGCT CCGGATCGGA CGATTGCGTC GCATCGACCC TGCGCCCAAG
  541 CTGCATCATC GAAATTGCCG TCAACCAAGC TCTGATAGAG TTGGTCAAGA CCAATGCGGA
  601 GCATATACGC CCGGAGCCGC GGCGATCCTG CAAGCTCCGG ATGCCTCCGC TCGAAGTAGC
  661 GCGTCTGCTG CTCCATACAA GCCAACCACG GCCTCCAGAA GAAGATGTTG GCGACCTCGT
  721 ATTGGGAATC CCCGAACATC GCCTCGCTCC AGTCAATGAC CGCTGTTATG CGGCCATTGT
  781 CCGTCAGGAC ATTGTTGGAG CCGAAATCCG CGTGCACGAG GTGCCGGACT TCGGGGCAGT
  841 CCTCGGCCCA AAGCATCAGC TCATCGAGAG CCTGCGCGAC GGACGCACTG ACGGTGTCGT
  901 CCATCACAGT TTGCCAGTGA TACACATGGG GATCAGCAAT CGCGCATATG AATCACGCCA
  961 TGTAGTGTAT TGACCGATTC CTTGCGGGTC CGAAATGGCC CGAACCCGCT TCGTTCTTGG
 1021 CTTAAGATCG GTCGCAGCGA TTCGCAATCA TGGACCTTCG CGACTGGCTG CAGAACAGCG
1081 GGCCAGTTCG GGATTTCCAG GGCCAGGGA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

2019.07.16

GNP_filter3_RDBDEP_html_190432.pl