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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK344 (Hygro-P2A-mAID)

A plasmid for construction of N-terminal Hygro-P2A-mAID tagging donors.

Catalog number RDB16805
Resource name pMK344 (Hygro-P2A-mAID)
Clone info. A plasmid for construction of N-terminal Hygro-P2A-mAID tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA
Depositor|Developer Kanemaki, Masato |
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External Database
Arabidopsis thaliana AtIAA17

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16805 pMK344 (Hygro-P2A-mAID) DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16805_A8Lpp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-M13_-100
          Sequence file: RDB16805_A8Lpa.seq check
          >D06157A1_A8LP_2_pUC-M13_-100_A01_01_ABI24.ab1
              1 GGACCTGTGG GCGGATTAGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC
             61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGGTA CCGGGCCCCC
            121 CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGCCACC ATGAAAAAGC CTGAACTCAC
            181 CGCGACGTCT GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA
            241 GCTCTCGGAG GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT
            301 CCTGCGGGTA AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT
            361 TGCATCGGCC GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT
            421 GACCTATTGC ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA
            481 ACTGCCCGCT GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT
            541 TAGCCAGACG AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG
            601 GCGTGATTTC ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA
            661 CGACACCGTC AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA
            721 CTGCCCCGAA GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA
            781 CAATGGCCGC ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA
            841 CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT TGTATGGAGC AGCAGACGCG
            901 CTACTTCGAG CGGGAGGCAT CCGGAGCTTT GCAGATCGCC CGCGGCTCCC GGGCGTATAT
            961 GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTTGTT GACGGCATTT CGAATGATGC
           1021 AGCCTTGGGC GCAGGGTTCG ATGCGACGCA TTCGGTTCGA TCGAAGCTGA ACTGGTCGGG
          1081 GCGTACACTA AAATTCGGTC CTGGTCA
          //
          Primer: reverse
          Sequence file: RDB16805_A8Lpb.seq check
          >D06157A1_A8LP_2_reverse_B01_04_ABI24.ab1
              1 CCCGGCAAAT TAAACCTCAC TAAAGGGACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG
             61 CTCTAGAACT AGTGGATCCG GCGCCTGCAC CAGCGCCTTT ATACATCCTC AAATCGATTT
            121 TCCTCAAGTA CGGTGCTCCG TCCATTGATA CCTTCACGAA CGCCGCCGCC TCCGGGCCAC
            181 CGCTTGATTT TTGGCAGGAA ACCATCACGT TCTTCCGGTA TGATCTCACC GGTGGCCATC
            241 CCACAACTTG TGCCTTGGCC GGAGGTTTGG CTGGATCTTT AGGACAAGCA CTCTTCTCCT
            301 TAGATCTAGG TCCAGGGTTC TCCTCCACGT CTCCAGCCTG CTTCAGCAGG CTGAAGTTAG
            361 TAGCTCCGCT TCCTGATTCC TTTGCCCTCG GACGAGTGCT GGGGCGTCGG TTTCCACTAT
            421 CGGCGAGTAC TTCTACACAG CCATCGGTCC AGACGGCCGC GCTTCTGCGG GCGATTTGTG
            481 TACGCCCGAC AGTCCCGGCT CCGGATCGGA CGATTGCGTC GCATCGACCC TGCGCCCAAG
            541 CTGCATCATC GAAATTGCCG TCAACCAAGC TCTGATAGAG TTGGTCAAGA CCAATGCGGA
            601 GCATATACGC CCGGAGCCGC GGCGATCCTG CAAGCTCCGG ATGCCTCCGC TCGAAGTAGC
            661 GCGTCTGCTG CTCCATACAA GCCAACCACG GCCTCCAGAA GAAGATGTTG GCGACCTCGT
            721 ATTGGGAATC CCCGAACATC GCCTCGCTCC AGTCAATGAC CGCTGTTATG CGGCCATTGT
            781 CCGTCAGGAC ATTGTTGGAG CCGAAATCCG CGTGCACGAG GTGCCGGACT TCGGGGCAGT
            841 CCTCGGCCCA AAGCATCAGC TCATCGAGAG CCTGCGCGAC GGACGCACTG ACGGTGTCGT
            901 CCATCACAGT TTGCCAGTGA TACACATGGG GATCAGCAAT CGCGCATATG AATCACGCCA
            961 TGTAGTGTAT TGACCGATTC CTTGCGGGTC CGAAATGGCC CGAACCCGCT TCGTTCTTGG
           1021 CTTAAGATCG GTCGCAGCGA TTCGCAATCA TGGACCTTCG CGACTGGCTG CAGAACAGCG
          1081 GGCCAGTTCG GGATTTCCAG GGCCAGGGA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          2021.11.02

          GNP_filter3_RDBDEP_html_211029.pl