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icon below as well as clone information before placing your order.
A plasmid for construction of N-terminal Hygro-P2A-mAID tagging donors.
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External Database Arabidopsis thaliana AtIAA17 Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
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| Ordering | Order form [Credit Card Payment ] [Bank Transfer Payment]MTA, for use for not-for-profit academic purpose [Word ]MTA, for use for for-profit purpose [Word ]Please visit Information of Request for Distribution.[link] |
[open/close]| 提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 |
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| 提供依頼 | 提供依頼書 [Word ]提供同意書 (MTA, 非営利学術目的用)[Word ]提供同意書 (MTA, 営利目的用)[Word ]手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB16805 | pMK344 (Hygro-P2A-mAID) | DNA solution |
icon below as well as clone information before placing your order.Materials & Methods section:
| The pMK344 (Hygro-P2A-mAID) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16805). |
Reference section:
| Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
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| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB16805_A8Lpp1-2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: pUC-M13_-100 (Pr0310) Region: T7 pro,HygR Sequence file: RDB16805_A8Lpc.seq
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>D06157A1_A8LP_2_pUC-M13_-100_A01_01_ABI24.ab1 1 NNNNNNNNNN NNNNNNNANN NNNNNNNNCC NNNGTTTTCC CNGTCACGAC GTTGTAAAAC 61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGGTA CCGGGCCCCC 121 CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGCCACC ATGAAAAAGC CTGAACTCAC 181 CGCGACGTCT GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA 241 GCTCTCGGAG GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT 301 CCTGCGGGTA AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT 361 TGCATCGGCC GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT 421 GACCTATTGC ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA 481 ACTGCCCGCT GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT 541 TAGCCAGACG AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG 601 GCGTGATTTC ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA 661 CGACACCGTC AGTGCGTCCG TCGCGCANGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA 721 CTGCCCCGAA GTCCGGCACC TCGTGCACGC GGATTTCNGC TCCNACAATG TCCTGACGGA 781 CAATGGCCGC ATAACAGCGG TCATTGNCTG GANCGAGGCG ATGTTCGGGG ATTCCCAATA 841 CGANGTCNCC AACNTCTTCN TCTGGANGNC GNGGTTGGCT TGNATGGAGC ANCANANNNN 901 CTANTTCNNN NNGNANGCNT NCNNANNNTN NNNNNNCGNN NNCNNCNNCN NGNNNNANNN 961 NNNNNNN // |
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Primer: reverse (Pr0099) Region: Linker,mAID,P2A,HygR Sequence file: RDB16805_A8Lpd.seq
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>D06157A1_A8LP_2_reverse_B01_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNGNANN NAAGCTGGAG CTCCNCCGCG GTGGCGGCCG 61 CTCTAGAACT AGTGGATCCG GCGCCTGCAC CAGCGCCTTT ATACATCCTC AAATCGATTT 121 TCCTCAAGTA CGGTGCTCCG TCCATTGATA CCTTCACGAA CGCCGCCGCC TCCGGGCCAC 181 CGCTTGATTT TTGGCAGGAA ACCATCACGT TCTTCCGGTA TGATCTCACC GGTGGCCATC 241 CCACAACTTG TGCCTTGGCC GGAGGTTTGG CTGGATCTTT AGGACAAGCA CTCTTCTCCT 301 TAGATCTAGG TCCAGGGTTC TCCTCCACGT CTCCAGCCTG CTTCAGCAGG CTGAAGTTAG 361 TAGCTCCGCT TCCTGATTCC TTTGCCCTCG GACGAGTGCT GGGGCGTCGG TTTCCACTAT 421 CGGCGAGTAC TTCTACACAG CCATCGGTCC AGACGGCCGC GCTTCTGCGG GCGATTTGTG 481 TACGCCCGAC AGTCCCGGCT CCGGATCGGA CGATTGCGTC GCATCGACCC TGCGCCCAAG 541 CTGCATCATC GAAATTGCCG TCAACCAAGC TCTGATAGAG TTGGTCAAGA CCAATGCGGA 601 GCATATACGC CCGGAGCCGC GGCGATCCTG CAAGCTCCGG ATGCCTCCGC TCGAAGTAGC 661 GCGTCTGCTG CTCCATACAA GCCAACCACG GCCTCCAGAA GAAGATGTTG GCGANCTCGT 721 ATTGGGAATC CCCGAACATC GCCTCGCTCC AGTCAATGAC CGCTGTTATG CGGCCATTGT 781 CCGTCANGAC ATTGTTGGAN CCGAAATCCG CGNGCACGAN GTGCCNGANT TCNGGGCAGT 841 CCTCGGNCNN AAGCATCNGC TCATCNNGAN NCTGCNCGAN GGANNCACTN NCGGNGNCGN 901 NCATCNNANN TNGNNANNGA TACNCNNNGG NNNCAGNNNT CGCGCATATN NNNCNCGCCN 961 NNNNNNNNN // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP] |
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2024.03.26
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