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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK344 (Hygro-P2A-mAID)

A plasmid for construction of N-terminal Hygro-P2A-mAID tagging donors.

Catalog number RDB16805
Resource name pMK344 (Hygro-P2A-mAID)
Clone info. A plasmid for construction of N-terminal Hygro-P2A-mAID tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA
Depositor|Developer Kanemaki, Masato |
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External Database
Arabidopsis thaliana AtIAA17

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB16805 pMK344 (Hygro-P2A-mAID) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16805_A8Lpp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-M13_-100
          Region: insert 5'(HygR 5')
          Sequence file: RDB16805_A8Lpc.seq check
          >D06157A1_A8LP_2_pUC-M13_-100_A01_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNANN NNNNNNNNCC NNNGTTTTCC CNGTCACGAC GTTGTAAAAC
             61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGGTA CCGGGCCCCC
            121 CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGCCACC ATGAAAAAGC CTGAACTCAC
            181 CGCGACGTCT GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA
            241 GCTCTCGGAG GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT
            301 CCTGCGGGTA AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT
            361 TGCATCGGCC GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT
            421 GACCTATTGC ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA
            481 ACTGCCCGCT GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT
            541 TAGCCAGACG AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG
            601 GCGTGATTTC ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA
            661 CGACACCGTC AGTGCGTCCG TCGCGCANGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA
            721 CTGCCCCGAA GTCCGGCACC TCGTGCACGC GGATTTCNGC TCCNACAATG TCCTGACGGA
            781 CAATGGCCGC ATAACAGCGG TCATTGNCTG GANCGAGGCG ATGTTCGGGG ATTCCCAATA
            841 CGANGTCNCC AACNTCTTCN TCTGGANGNC GNGGTTGGCT TGNATGGAGC ANCANANNNN
            901 CTANTTCNNN NNGNANGCNT NCNNANNNTN NNNNNNCGNN NNCNNCNNCN NGNNNNANNN
          961 NNNNNNN
          //
          Primer: reverse
          Region: insert 3'(linker,mAID,P2A,HygR 3')
          Sequence file: RDB16805_A8Lpd.seq check
          >D06157A1_A8LP_2_reverse_B01_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNGNANN NAAGCTGGAG CTCCNCCGCG GTGGCGGCCG
             61 CTCTAGAACT AGTGGATCCG GCGCCTGCAC CAGCGCCTTT ATACATCCTC AAATCGATTT
            121 TCCTCAAGTA CGGTGCTCCG TCCATTGATA CCTTCACGAA CGCCGCCGCC TCCGGGCCAC
            181 CGCTTGATTT TTGGCAGGAA ACCATCACGT TCTTCCGGTA TGATCTCACC GGTGGCCATC
            241 CCACAACTTG TGCCTTGGCC GGAGGTTTGG CTGGATCTTT AGGACAAGCA CTCTTCTCCT
            301 TAGATCTAGG TCCAGGGTTC TCCTCCACGT CTCCAGCCTG CTTCAGCAGG CTGAAGTTAG
            361 TAGCTCCGCT TCCTGATTCC TTTGCCCTCG GACGAGTGCT GGGGCGTCGG TTTCCACTAT
            421 CGGCGAGTAC TTCTACACAG CCATCGGTCC AGACGGCCGC GCTTCTGCGG GCGATTTGTG
            481 TACGCCCGAC AGTCCCGGCT CCGGATCGGA CGATTGCGTC GCATCGACCC TGCGCCCAAG
            541 CTGCATCATC GAAATTGCCG TCAACCAAGC TCTGATAGAG TTGGTCAAGA CCAATGCGGA
            601 GCATATACGC CCGGAGCCGC GGCGATCCTG CAAGCTCCGG ATGCCTCCGC TCGAAGTAGC
            661 GCGTCTGCTG CTCCATACAA GCCAACCACG GCCTCCAGAA GAAGATGTTG GCGANCTCGT
            721 ATTGGGAATC CCCGAACATC GCCTCGCTCC AGTCAATGAC CGCTGTTATG CGGCCATTGT
            781 CCGTCANGAC ATTGTTGGAN CCGAAATCCG CGNGCACGAN GTGCCNGANT TCNGGGCAGT
            841 CCTCGGNCNN AAGCATCNGC TCATCNNGAN NCTGCNCGAN GGANNCACTN NCGGNGNCGN
            901 NCATCNNANN TNGNNANNGA TACNCNNNGG NNNCAGNNNT CGCGCATATN NNNCNCGCCN
          961 NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          2022.07.06

          GNP_filter3_RDBDEP_html_220520.pl