Expression clone for an auxin-based degron (AID) system, aid-CENPH.
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Other clones in our bank
External Database Gallus gallus CENPH Oryza sativa OsTIR1 Arabidopsis thaliana AtIAA17 Reference sequence |
Sequence | RDB08469z.seq |
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Remarks, protocol and/or map (pdf) | RDB08469.pdf |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Nishimura, K., Nat. Methods., 6, 917-922 (2009)). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
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Ordering | Order form [Credit Card Payment ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() MTA, for use for for-profit purpose [Word ![]() Please visit Information of Request for Distribution.[link] |
提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishimura, K., Nat. Methods., 6, 917-922 (2009))。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 |
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提供依頼 | 提供依頼書 [Word ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() 提供同意書 (MTA, 営利目的用)[Word ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB08469 | pMK106 | DNA solution |
Materials & Methods section:
The pMK106 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08469). |
Reference section:
Nishimura, K., Fukagawa, T., Takisawa, H., Kakimoto, T., Kanemaki, M., An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods. 6(12), 917-922, (2009). PMID 19915560. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Auxin Inducible Degron (AID) System (English text) |
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Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18378_B0Hhp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV_Forward (Pr0016) Region: CMV pro,OsTIR1 5' Sequence file: RDB18378_B0Hha.seq ![]() |
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>08469_18378_B0Hh_3_CMV_Forward_A11_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNTNNN GCNNATGGGC GGTANGCGTG TACGGTGGGN NGTCTATATA 61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGACTCA GATCTCGAGA 121 TGACGTACTT CCCGGAGGAG GTGGTGGAGC ACATCTTCAG CTTCCTGCCG GCGCAGCGCG 181 ACCGCAACAC GGTCTCGCTC GTCTGCAAGG TGTGGTACGA GATCGAGAGG CTGAGCCGCC 241 GCGGCGTCTT CGTGGGCAAC TGCTACGCCG TGCGCGCCGG CCGCGTCGCC GCGCGGTTCC 301 CCAACGTGCG GGCGCTCACG GTGAAGGGGA AGCCCCACTT CGCCGACTTC AACCTCGTGC 361 CCCCCGACTG GGGCGGCTAC GCGGGGCCGT GGATCGAGGC GGCCGCGAGG GGATGCCACG 421 GCCTGGAGGA GCTCAGGATG AAGCGGATGG TGGTGTCCGA CGAGAGCCTC GAGCTGCTGG 481 CTCGCTCGTT CCCGCGGTTC AGGGCTCTTG TTCTTATCAG CTGCGAGGGG TTCAGCACTG 541 ACGGGCTAGC CGCCGTCGCG AGCCATTGCA AGCTTCTGAG GGAGTTGGAT TTGCAGGAAA 601 ATGAAGTGGA GGATCGAGGG CCTAGGTGGC TTTCCTGCTT CCCTGATTCC TGCACATCAC 661 TTGTCTCATT GAATTTTGCC TGCATCAAAG GGGANGTTAA TGCTGGTTCA CTGGAGAGAC 721 TTGTTAGCAG GTCCNCAAAC CTGCGGANTT TGANGCTGAN TCGATCTGTA TCGGTAGATA 781 CACTTGCNAA GATACTACTG CGTACNNCTA ACTTGNNNGA NTTGGGGANA NGGAATTTGN 841 CAGATGANTT CCNAACTGAN TCCTANTTTN AGCTTACCAN NNCTCTGGAN AANTGNNANA 901 NGTTGANGAN TTTGTCNGGN NTCTGGNNNN NNTCNNCNNG TTNNNNNNNN NN // |
Primer: IRES-R (Pr0037) Region: 9XMyc,OaTIR1 3' Sequence file: RDB18378_B0Hhb.seq ![]() |
>08469_18378_B0Hh_3_IRES-R_C11_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNGTTA NGGGGGGGGG NNGGNNNNGG GCGGNTCCCG 61 GGGNNCTCTT GAATGATCGT TCCACTTTTT AGCTAGTGGA TCCGTTCAAG TCTTCTTCTG 121 AGATTAATTT TTGTTCACCG TTCAAGTCTT CCTCGGAGAT TAGCTTTTGT TCACCGTTCA 181 AATCTTCTTC AGAAATCAAC TTTTGTTCAC CTCTAGAGGA TCCGTTCAAG TCTTCTTCTG 241 AGATTAATTT TTGTTCACCG TTCAAGTCTT CCTCGGAGAT TAGCTTTTGT TCACCGTTCA 301 AATCTTCTTC AGAAATCAAC TTTTGTTCAC CTCTAGAGGA TCCGTTCAAG TCTTCTTCTG 361 AGATTAATTT TTGTTCACCG TTCAAGTCTT CCTCGGAGAT TAGCTTTTGT TCACCGTTCA 421 AATCTTCTTC AGAAATCAAC TTTTGTTCAC CACTAGCAGC AGAACCGGAG TCGACTAGGA 481 TTTTAACAAA ATTTGGTGCA TCATCCCTCG CCCCAGCAGT TGTGCGGTAC ACATATAATT 541 TCTCCACTTT AGGCAGATCT CCATGGTTTT CCTCCATTTC ATTGCTACCA TCCCGCTCAT 601 TTATGACCTC AACATTGAGC ATCGGCATCT TTGACGCAAG GACTTGGCAC CCCTTTAACG 661 TGACATTGCA AGATGACATC CAAAGGGATC GCATTGTCTC GTACCTAGCA AAATTCCCCA 721 AGAGTGCAGC ATCACCAAAC GGGCTATCTC TTATCTCCNG TTTCCTTAAA TTCTTGCATC 781 CATTCATAAC ATGCATCATA CCCTTATCAC TGTCACCAGC AAATGCTATA NAAAGCATCT 841 CNAGTTGTTT TGCATATTTC CNAATATACA TGAAAACTTT GTCTGTGAGA NNANCAGATA 901 TTGANAAACG TTNNNATCCC NNGCACNCNC NAACNNNANN NNCNAANNNT TNNTCNNATG 961 GTNNNNTTGN CNCNNNNTCN GNNTNNCNNG GNNNNNNNAN NNNNANNNCN GNANNNNNNN 1021 NNN // |
Primer: IRES-F (Pr0036) Region: IRES2,AID 5' Sequence file: RDB18378_B0Hhc.seq ![]() |
>08469_18378_B0Hh_3_IRES-F_D11_11_ABI24.ab1 1 NNNNNNNNNN NNNNTNNNTC GNNGTTNNNA AACGTCTAGG CCCCCCGAAC CNCGGGGNCG 61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGGGCAG TGTCGAGCTG 121 AATCTGAGGG AGACTGAGCT GTGTCTTGGT CTTCCCGGTG GAGATACAGT GGCTCCGGTA 181 ACCGGAAACA AGAGAGGGTT CTCAGAGACG GTTGATCTGA AGCTAAATCT GAATAATGAG 241 CCTGCAAACA AGGAAGGATC TACGACTCAT GACGTCGTGA CTTTTGATTC CAAGGAGAAG 301 AGTGCTTGTC CTAAAGATCC AGCCAAACCT CCGGCCAAGG CACAAGTTGT GGGATGGCCA 361 CCGGTGAGAT CATACCGGAA GAACGTGATG GTTTCCTGCC AAAAATCAAG CGGTGGCCCG 421 GAGGCGGCGG CGTTCGTGAA GGTATCAATG GACGGAGCAC CGTACTTGAG GAAAATCGAT 481 TTGAGGATGT ATAAAAGCTA CGATGAGCTT TCTAATGCTT TGTCCAACAT GTTCAGCTCT 541 TTTACCATGG GCAAACATGG AGGAGAAGAA GGAATGATAG ACTTCATGAA TGAGAGGAAA 601 TTGATGGATT TGGTGAATAG CTGGGACTAT GTTCCCTCTT ATGAAGACAA AGACGGTGAT 661 TGGATGCTCG TCGGCGACGT TCCTTGGCCA ATGTTCGTCG ATACATGCAA GCGTTTACGT 721 CTCATGAAAG GATCGGATGC CATTGGTCTC GCTCCGAGGG CGATGGAGAA GTGCAAGANC 781 AGANCTGCGG CCGGANCTGG TGCANGCGCT GGANCGGGTG CCGATATCGA ATTCACCANG 841 GCGGGGCGGC TGTCGGANNN CGTCNGGNNC NGGCCGGGCG CTNANGCCNA NACNGCGGCC 901 NATNCNNNNG CNAANCGGGN NNNNNNNNN // |
Primer: SV40polyA_R2 (Pr0623) Region: bGHpA,chiken CENP-H 3' Sequence file: RDB18378_B0Hhd.seq ![]() |
>08469_18378_B0Hh_3_SV40polyA_R2_B11_05_ABI24.ab1 1 NNNNNNNNCN NNNNNNNNNN NGTTNNNAAC AACAATTGCA TTCNTTTTAT GTTTCAGGTT 61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT 121 GATTATGATC TAGAGTCGCG GCCGCAGTGA TCACAGTACT CTACAAGAGA TTCTGAATGG 181 AGATATCTTT CTCGAGCTGT AGAATAATTG CTCTTAAAGA TGGATCTTTC GCCCAGTTGA 241 TTTGGCATCC GACTATGATA TTCTGGAGTA TATTTTGAAC TAACGTAGTC ACCTTCCTTT 301 CATACTGTAC ATTCCTATGG ATAAACTTCA ACTTTTCAGC AATTTTCATT CTGACTACTT 361 CCTTTTTTTT CCTCACTGTG GTAAAAATTC GCAGCGCCTT CTCTCCTTCG GCCTGTTTCA 421 GAAAGTATCT TTTCCTTCTA ATGTCATTCA AATTCTCTCT AATCACACGT GCTTGCTGAT 481 GAGCTTGAAT TATTTCCCAG TTGAGCTTGA TTATGTCTCT CATTGTTTCC ACGATCAGTC 541 GTGCGCAACC ATCATTTTCT TTCACTCTCT TTCTCAGAGC ATCCATAAGC TGGATCCTTT 601 TTATGACTAA TGCTTTGCTT TCAAAGGCAC TTTTTACTTT CCCCACTTCT TCCAAAGTTT 661 CTTTGGCACT TTCGATAAAG TTTACTTCCG CGCCACTGTT GCACCCTTCA TCGGGGGGGC 721 ACGCGGTGTC GAACTCCATC ACCAGCTGCT TCAGATGCGT CCTCGCGCAN ANGTGCTCCA 781 GGACGTCCCG CTTCGCGTCC GGNTCGGCCG CCGTCTCGGC CTCAGCGCCC GGCCCGGANC 841 CNANGGNNTC CGACAGCCGC CCCGCCANGG NGAANTNNAT ATCGGCACCN GCNCCAGCGC 901 NNGNNCCANC TCCNGNCGCA NCNNNGCNNN NGCNNTTNNN CNTCNNCCTC NNNNCNNNNN 961 NNNNGNNNNN NNNNN // |
Primer: CENP-M_R (Pr0887) Region: chiken CENP-H 5',AID 3' Sequence file: RDB18378_B0Hhe.seq ![]() |
>08469_18378_B0Hh_3_CENP-M_R_E11_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNTCNCNN GCTGCTTNNG ATGCGTCCTC GCGCAGANGT 61 GCTCCAGGAC GTCCCGCTTC GCGTCCGGAT CGGCCGCCGT CTCGGCCTCA GCGCCCGGCC 121 CGGACCCGAC GGACTCCGAC AGCCGCCCCG CCATGGTGAA TTCGATATCG GCACCCGCTC 181 CAGCGCCTGC ACCAGCTCCG GCCGCAGCTC TGCTCTTGCA CTTCTCCATC GCCCTCGGAG 241 CGAGACCAAT GGCATCCGAT CCTTTCATGA GACGTAAACG CTTGCATGTA TCGACGAACA 301 TTGGCCAAGG AACGTCGCCG ACGAGCATCC AATCACCGTC TTTGTCTTCA TAAGAGGGAA 361 CATAGTCCCA GCTATTCACC AAATCCATCA ATTTCCTCTC ATTCATGAAG TCTATCATTC 421 CTTCTTCTCC TCCATGTTTG CCCATGGTAA AAGAGCTGAA CATGTTGGAC AAAGCATTAG 481 AAAGCTCATC GTAGCTTTTA TACATCCTCA AATCGATTTT CCTCAAGTAC GGTGCTCCGT 541 CCATTGATAC CTTCACGAAC GCCGCCGCCT CCGGGCCACC GCTTGATTTT TGGCAGGAAA 601 CCATCACGTT CTTCCGGTAT GATCTCACCG GTGGCCATCC CACAACTTGT GCCTTGGCCG 661 GAGGTTTGGC TGGATCTTTA GGACAAGCAC TCTTCTCCTT GGAATCAAAA GTCACGACGT 721 CATGAGTCGT AGATCCTTCC TTGTTTGCAG GCTCATTATT CAGATTTAGC TTCAGATCAA 781 CCGTCTCTGA NAACCCTCTC TTGTTTCCGG TTACCGGANC CACTGTATCT CCACCGGGAA 841 NANCAAGANA CAGCTCANTC TCCNTCANAN TCAGCTCNAC ACNGCCCANG GNTGNGGNCN 901 TATNATCATC NNGNTTTTTC NAANGNAAAN CACNNNCCCN NNNNNTNCNG GGGGGNCNNN 961 NNNNNTTTTT TNNNNCNNNN NNNNANNNNN NNNNANNNNN NNNNN // |
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR_KanR Sequence file: RDB18378_B0Hhf.seq ![]() |
>08469_18378_B0Hh_3_SV40pro_ori_F_F11_17_ABI24.ab1 1 NNNNNNNNNN NNNNGNNGCC TCNGCCTCTG NNCTATTCNN GAAGTAGTGA GGAGGCTTTT 61 TTGGAGGCCT AGGCTTTTGC AAAGATCGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 121 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 181 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 241 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAA 301 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 361 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 421 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 481 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 541 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 601 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT GCCCGACGGC 661 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 721 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA TCANGACATA 781 GCGTTGGCTA CNNGTGATAT TGCTGAAGAN CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 841 GNGCTTTACN GNATCGCCGC TCCCNANTCN CANCGCATCG CCTTCNATCG CNNTCNNGAN 901 NANNNNNTCN NNNCGGNNNN NNNGGNNNNC NAAANNNNNN NNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Nishimura, K., An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods. 6(12), 917-922, (2009). PMID 19915560. [link to RRC of NBRP] |
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reference | Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318. |
reference | Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302. |
reference | Holland, A.J., Inducible, reversible system for the rapid and complete degradation of proteins in mammalian cells. Proc. Natl. Acad. Sci. U.S.A. 109 (49): E3350-7 PMID 23150568. |
user_report | Hatoyama, Y., Establishment and analysis of conditional Rab1- and Rab5-knockout cells using the auxin-inducible degron system. J. Cell Sci. 134 (24): jcs259184 (2021). PMID 34817057. [link to RRC of NBRP] |
user_report | Miura, K., Application of auxin-inducible degron technology to mouse oocyte activation with PLCzeta. J. Reprod. Dev. 64 (4): 319-326 (2018). PMID 29731504. [link to RRC of NBRP] |
2023.10.05
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