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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

RAD21 C-tag CIRPSR

Expression vector of Cas9 and gRNA for a RAD21 tagging donor.

Catalog number RDB18346
Resource name RAD21 C-tag CIRPSR
Clone info. Expression vector of Cas9 and gRNA for a RAD21 tagging donor, see Fig. 3 of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone px330 (plasmid)
Selectable markers Ampicillin
Gene/insert name human RAD21 cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
human RAD21

          Reference sequence
            
           
          Sequence (full) RDB18346zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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          Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
          Remarks
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word]
          備考
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB18346 RAD21 C-tag CIRPSR DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18346_B0Gfp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: SV40pA3element Reverse
          Region: U6 pro,sgRNA for RAD21,CBh pro
          Sequence file: RDB18346_B0Gfa.seq check
          >D07105B5_B0Gf_4_SV40pA3'element Reverse_A04_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGNNNNNNG CNNGCAACGC GGCCTTTTTA CGGTTCCTGG
             61 CCTTTNNNNN NNNNNTTGCT CACATGTGAG GGCCTATTTC CCATGATTCC TTCATATTTG
            121 CATATACGAT ACAAGGCTGT TAGAGAGATA ATTGGAATTA ATTTGACTGT AAACACAAAG
            181 ATATTAGTAC AAAATACGTG ACGTAGAAAG TAATAATTTC TTGGGTAGTT TGCAGTTTTA
            241 AAATTATGTT TTAAAATGGA CTATCATATG CTTACCGTAA CTTGAAAGTA TTTCGATTTC
            301 TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACACCGCCA AGGTTCCATA TTATATAGTT
            361 TTAGAGCTAG AAATAGCAAG TTAAAATAAG GCTAGTCCGT TATCAACTTG AAAAAGTGGC
            421 ACCGAGTCGG TGCTTTTTTG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC
            481 GTTTTTAGCG CGTGCGCCAA TTCTGCAGAC AAATGGCTCT AGAGGTACCC GTTACATAAC
            541 TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAG
            601 TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC
            661 ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG
            721 GTAAATGGCC CGCCTGGCAT TGTGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC
            781 AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTCGA NGTGAGCCCC ACGTTCTGCT
            841 TCACTCTCCC CATCTCCCCC CCCCNCNCCA NCCCCAATTT TGTATTTATT TATTTTTTNN
          901 ATTATTTTGT GCANCGATGG GGGCNNNNNN NNNN
          //
          Primer: b-actin pro_F1
          Region: 3xFLAG,SV40NLS,Cas9
          Sequence file: RDB18346_B0Gfb.seq check
          >D07105B5_B0Gf_4_b-actin pro_F1_C06_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGTGGGGNN NTAATGTTTA ATTACCTGGN
             61 NNNNNNGCCT GAANTCNNTT TTTTTCNGGT TGGACCGGTG NCNCCATGGA CTATAAGGAC
            121 CACGACGGAG ACTACAAGGA TCATGATATT GATTACAAAG ACGATGACGA TAAGATGGCC
            181 CCAAAGAAGA AGCGGAAGGT CGGTATCCAC GGAGTCCCAG CAGCCGACAA GAAGTACAGC
            241 ATCGGCCTGG ACATCGGCAC CAACTCTGTG GGCTGGGCCG TGATCACCGA CGAGTACAAG
            301 GTGCCCAGCA AGAAATTCAA GGTGCTGGGC AACACCGACC GGCACAGCAT CAAGAAGAAC
            361 CTGATCGGAG CCCTGCTGTT CGACAGCGGC GAAACAGCCG AGGCCACCCG GCTGAAGAGA
            421 ACCGCCAGAA GAAGATACAC CAGACGGAAG AACCGGATCT GCTATCTGCA AGAGATCTTC
            481 AGCAACGAGA TGGCCAAGGT GGACGACAGC TTCTTCCACA GACTGGAAGA GTCCTTCCTG
            541 GTGGAAGAGG ATAAGAAGCA CGAGCGGCAC CCCATCTTCG GCAACATCGT GGACGAGGTG
            601 GCCTACCACG AGAAGTACCC CACCATCTAN NNNNNGAGAA AGAAACTGGT GGACAGCACC
            661 GACAAGGCCG ACCTGCGGCT GATCTATCTG GCCCTGNNNN NNATGATCAA GTTCCGGGGC
            721 CACTTCCTGA TCGAGGGCGA CCTGAACCCC GACAACANNN NNNTGGACNA AGCTGTTCNT
            781 CCAGCTGGTG NANANCTACA NCCNGCTGTT CGAGGNAAAA CCCCATCNAN NNCCAGCGGC
            841 GTGGNACGCC NNNNGCCATC NNNNTCTGCC AGACTGANNN ANNANCANAN NGGCNGGAAA
            901 ATNNNNNNNC CCANNTGNCC NGCNNNGNAN AANANNGGNN NNNGTNNNGN ANNNNNNNNN
          961 N
          //
          Primer: bGH_rev3
          Region: bGH pA,nucleoplasmin NLS,Cas9
          Sequence file: RDB18346_B0Gfc.seq check
          >D07105B5_B0Gf_4_bGH_rev3_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGNNAAACAA CNGATGGCTG GCAACTAGAA
             61 GGCACAGTCG AGGCTGATCA GCGAGCTCTA GGAATTCTTA CTTTTTCTTT TTTGCCTGGC
            121 CGGCCTTTTT CGTGGCCGCC GGCCTTTTGT CGCCTCCCAG CTGAGACAGG TCGATCCGTG
            181 TCTCGTACAG GCCGGTGATG CTCTGGTGGA TCAGGGTGGC GTCCAGCACC TCTTTGGTGC
            241 TGGTGTACCT CTTCCGGTCG ATGGTGGTGT CAAAGTACTT GAAGGCGGCA GGGGCTCCCA
            301 GATTGGTCAG GGTAAACAGG TGGATGATAT TCTCGGCCTG CTCTCTGATG GGCTTATCCC
            361 GGTGCTTGTT GTAGGCGGAC AGCACTTTGT CCAGATTAGC GTCGGCCAGG ATCACTCTCT
            421 TGGAGAACTC GCTGATCTGC TCGATGATCT CGTCCAGGTA GTGCTTGTGC TGTTCCACAA
            481 ACAGCTGTTT CTGCTCATTA TCCTCGGGGG AGCCCTTCAG CTTCTCATAG TGGCTGGCCA
            541 GGTACAGGAA GTTCACATAT TTGGAGGGCA GGGCCAGTTC GTTTCCCTTC TGCAGTTCGC
            601 CGGCAGAGGC CAGCATTCTC TTCCGGCCGT TTTCCAGCTC GAACAGGGAG TACTTAGGCA
            661 GCTTGATGAT CAGGTCCTTT TTCACTTCTT TGTAGCCCTT GGCTTCCAGA AAGTCGATGG
            721 GATTCTTCTC NAAGCTGCTT CTTTCCATGA TGGTGATCCC CANCAGCTCT TTCACACTCT
            781 TCAGTTTCTT GGACTTGCCC TTTTNCACTT TGGCCNCCAC CAGCACANNN NNNGNCACNG
            841 TGGGGCTGTC GAANCCGCCN TACTTCTTNN GNNCCCNNNN CTTCNTTCTG GNNANCAGCT
            901 TATCNNTGNT NNCTCTTGGG CNNGANANAN TCTTTNNCTG ANNCCGCCNG TCNGCNNCTC
          961 GNNCNTTTNN ACNNNNNTCN CNTGGGNNAN GNTCNNNNNN TNCNNNNNNN NNN
          //
          Primer: Cas9-N_R1
          Region: SV40 NLS,3xFLAG,CBh pro
          Sequence file: RDB18346_B0Gfd.seq check
          >D07105B5_B0Gf_4_Cas9-N_R1_C04_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNTGNNNT CGTCGGTGNN CACGGCCCAG CCCACAGAGT
             61 TGGTGCNNNN NNNNNNNNNN ATGCTGTACT TCTTGTCGGC TGCTGGNACT NNNTGGATAC
            121 CGACCTTCCG CTTCTTCTTT GGGGCCATCT TATCGTCATC GTCTTTGTAA TCAATATCAT
            181 GATCCTTGTA GTCTCCGTCG TGGTCCTTAT AGTCCATGGT GGCACCGGTC CAACCTGAAA
            241 AAAAGTGATT TCAGGCAGGT GCTCCAGGTA ATTAAACATT AATACCCCAC CAACCAACCA
            301 TCCCTTAAAC CCTTACCTCT TGCTCAGCTA ATTACAGCCC GGAGGAGAAG GGCCGTCCCG
          361 CCCGCTCACC TGTGGGAGTA ACGCGGTCAG TCAGAGCCGG GGCGGGCGGN GNGAGGNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

          2022.09.29

          GNP_filter3_RDBDEP_html_220812.pl