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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK283 (Stag-3FLAG-Neo)

A plasmid for construction of C-terminal Stag-3FLAG tagging donors.

Catalog number RDB13926
Resource name pMK283 (Stag-3FLAG-Neo)
Alternative name Stag-3FLAG-Neo
Clone info. A plasmid for construction of C-terminal Stag-3FLAG tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone. Please note that this plasmid does not contain the loxP sites, which are intended for removal of the marker.
Comment Sequence analysis is not reliable around MluI site adjacent to SV40 poly(A) site probably because of tangled tertiary structure induced by unexpected palindrome sequences.
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Depositor|Developer Kanemaki, Masato |
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            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB13926 pMK283 (Stag-3FLAG-Neo) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Auxin Inducible Degron (AID) System (English text)
            Featured content Auxin Inducible Degron (AID) System (Japanese text)
            Featured content Genome Editing (English text)
            Featured content Genome Editing (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13926_A6B8p1_v2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13_-40
            Sequence file: RDB13926_A6B8a_2.seq check
            >D03070A10_A6B8_3_M13(-40)_B01_04.ab1
                1 CAGCCAGCGC GCGCGTATAC GACTCACTAT AGGGCGAATT GGAGCTCGGA TCCGGTGCAG
               61 GCGCCAAGGA AACCGCCGCT GCAAAATTCG AACGCCAGCA CATGGACAGC CGATCCCGGG
              121 CTGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG
              181 ATGACAAGTA GTGTACAAGT AATCTAGATA ACTGATCATA ATCAGCCATA CCACATTTGT
              241 AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT
              301 GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
              361 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC
            421 CAAACTCATC AATGTATCTT AACGCGTAAA TTGTAAGCG
            //
            Primer: SV40pro_F_V2
            Sequence file: RDB13926_A6B8b_2.seq check
            >D03070A10_A6B8_3_SV40pro_F_V2_H09_24.ab1
                1 GTAGGCTTTT TGGAGGCTAG GCTTTTGCAA GATCGATCAA GAGACAGGAT GAGGATCGTT
               61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
              121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
              181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
              241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
              301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
              361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
              421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
              481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
              541 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
              601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
              661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
              721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
              781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
              841 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC
              901 AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA
              961 ATCGTTTTCC CGGGACGCCG GCTGGATGAT CCCTCCAGCG CGGGGGATCT CATGCTGGGA
             1021 GGTTCTTTCG CCCACCCCTA GGGGGAGGCT ACTGAAACAC GGATGAGAAC ATACCGAGAA
            1081 CCCGCGCTAT GACGCCAATA AAGACGAGTA AAACGGGACT AACT
            //
            Primer: M13R
            Sequence file: RDB13926_A6B8c_2.seq check
            >D03070A10_A6B8_3_M13R_A01_01.ab1
                1 CTGGGGCACT ACCCTCACTA AAGGGAACAA AAGCTGGGTA CCGGGCCCCC CCTCGAGGGA
               61 TCCAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT
              121 CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC
              181 TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG
              241 AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC
              301 GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC
              361 GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT
              421 CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT
              481 AGCGGTCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA
              541 CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA
              601 TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA
              661 GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT
              721 TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT
              781 CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG
              841 GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG
              901 CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCCTCGTC TTGCAGTTCA
              961 TTCAGGGCAC CCGGACAGGT CGGTCTTGAC AAAAAGAACC CGGGCGCCCC CTGCGCTGAC
             1021 AGCCCGGGAA CGCGCGGCAT CAGAAGCAGC CCGATTTGTC TGTTGTGCCC AGTCATAAGC
            1081 CGATAGCCTC TTCCACCCAG CGGCCGGGAG AAAACCCTTG GCGGTTGCAA CATTC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

            2022.05.17

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