Sequencing and PCR primers for primer information.
A plasmid for construction of C-terminal Stag-3FLAG tagging donors.
| Catalog number | RDB13926 |
|---|---|
| Resource name | pMK283 (Stag-3FLAG-Neo) |
| Alternative name | Stag-3FLAG-Neo |
| Clone info. | A plasmid for construction of C-terminal Stag-3FLAG tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone. Please note that this plasmid does not contain the loxP sites, which are intended for removal of the marker. Sequence analysis is not reliable around MluI site adjacent to SV40 poly(A) site probably because of tangled tertiary structure induced by unexpected palindrome sequences. |
| Vector backbone | pBluescriptII KS- (Plasmid) |
| Size of vector backbone | 3.0 kb |
| Selectable markers | Am^r |
| Depositor | Kanemaki, Masato | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB13926 | pMK283 (Stag-3FLAG-Neo) | DNA solution |
Electronic file
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
|---|---|
| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
| Featured content | Genome Editing (English text) |
| Featured content | Genome Editing (Japanese text) |
| Featured content | Empty Backbone (English text) |
| Featured content | Empty Backbone (Japanese text) |
References
| original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
|---|
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13926_A6B8p1_v2.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
| Primer: M13_-40 | RDB13926_A6B8a_2.seq |
|---|---|
|
>D03070A10_A6B8_3_M13(-40)_B01_04.ab1 1 CAGCCAGCGC GCGCGTATAC GACTCACTAT AGGGCGAATT GGAGCTCGGA TCCGGTGCAG 61 GCGCCAAGGA AACCGCCGCT GCAAAATTCG AACGCCAGCA CATGGACAGC CGATCCCGGG 121 CTGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG 181 ATGACAAGTA GTGTACAAGT AATCTAGATA ACTGATCATA ATCAGCCATA CCACATTTGT 241 AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT 301 GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA 361 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC 421 CAAACTCATC AATGTATCTT AACGCGTAAA TTGTAAGCG // |
|
| Primer: SV40pro_F_V2 | RDB13926_A6B8b_2.seq |
|
>D03070A10_A6B8_3_SV40pro_F_V2_H09_24.ab1 1 GTAGGCTTTT TGGAGGCTAG GCTTTTGCAA GATCGATCAA GAGACAGGAT GAGGATCGTT 61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT 121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT 181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA 241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC 301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG 361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC 421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA 481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA 541 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC 601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA 661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA 721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG 781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT 841 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC 901 AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA 961 ATCGTTTTCC CGGGACGCCG GCTGGATGAT CCCTCCAGCG CGGGGGATCT CATGCTGGGA 1021 GGTTCTTTCG CCCACCCCTA GGGGGAGGCT ACTGAAACAC GGATGAGAAC ATACCGAGAA 1081 CCCGCGCTAT GACGCCAATA AAGACGAGTA AAACGGGACT AACT // |
|
| Primer: M13R | RDB13926_A6B8c_2.seq |
|
>D03070A10_A6B8_3_M13R_A01_01.ab1 1 CTGGGGCACT ACCCTCACTA AAGGGAACAA AAGCTGGGTA CCGGGCCCCC CCTCGAGGGA 61 TCCAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT 121 CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC 181 TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG 241 AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC 301 GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC 361 GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT 421 CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT 481 AGCGGTCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA 541 CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA 601 TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA 661 GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT 721 TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT 781 CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG 841 GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG 901 CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCCTCGTC TTGCAGTTCA 961 TTCAGGGCAC CCGGACAGGT CGGTCTTGAC AAAAAGAACC CGGGCGCCCC CTGCGCTGAC 1021 AGCCCGGGAA CGCGCGGCAT CAGAAGCAGC CCGATTTGTC TGTTGTGCCC AGTCATAAGC 1081 CGATAGCCTC TTCCACCCAG CGGCCGGGAG AAAACCCTTG GCGGTTGCAA CATTC // |
|
Please visit Sequencing and PCR primers for primer information.
References
| original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. |
|---|
2018.09.03
GNP_filter3_RDBDEP_html_180823.pl