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pMK364 (CMV-OsTIR1-loxP-PURO-loxP)

A donor vector for introducing CMV-OsTIR1 at the human AAVS1 locus.

Catalog number RDB16811
Resource name pMK364 (CMV-OsTIR1-loxP-PURO-loxP)
Clone info. A donor vector for introducing CMV-OsTIR1 at the human AAVS1 locus. Oryza sativa OsTIR1 (NP_001052659.1), codon optimized cDNA for the expression in human cells.
Vector backbone plasmid
Selectable markers Am^r (E. coli), Puro^r (mammalian cells)
Gene/insert name human AAVS1 genomic Oryza sativa OsTIR1 cDNA
Depositor|Developer Kanemaki, Masato |
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External Database
human AAVS1 Oryza sativa OsTIR1

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB16811 pMK364 (CMV-OsTIR1-loxP-PURO-loxP) DNA solution

        Ordering Information [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Auxin Inducible Degron (AID) System (English text)
        Featured content Auxin Inducible Degron (AID) System (Japanese text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB16811_A8Lpp1-3.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV-Forward
        Region: CMV pro, insert 5'
        Sequence file: RDB16811_A8Lpa.seq check
        >D06157A7_A8LP_1_CMV-Forward_D03_12_ABI24.ab1
            1 ATGTTCCTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
           61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTG CCACCATGAC ATACTTTCCT
          121 GAAGAGGTCG TCGAACACAT TTTTAGCTTC CTGCCTGCAC AGAGAGATAG AAACACAGTG
          181 AGCCTGGTCT GCAAAGTGTG GTACGAGATC GAACGCCTGA GCCGGAGAGG AGTGTTCGTC
          241 GGCAACTGCT ATGCTGTGAG AGCAGGCAGG GTCGCCGCTA GGTTTCCAAA TGTGCGCGCA
          301 CTGACCGTCA AGGGGAAACC CCACTTCGCC GACTTTAACC TGGTGCCCCC TGATTGGGGA
          361 GGATACGCCG GCCCTTGGAT CGAGGCAGCC GCTCGCGGCT GTCATGGACT GGAGGAACTG
          421 CGCATGAAGC GAATGGTGGT CTCTGACGAA AGTCTGGAGC TGCTGGCTCG GAGCTTCCCT
          481 AGGTTTCGCG CACTGGTGCT GATTTCTTGC GAAGGCTTCA GCACCGATGG ACTGGCAGCC
          541 GTGGCCTCCC ACTGTAAGCT GCTGCGGGAG CTGGACCTCC AGGAGAATGA AGTGGAGGAT
          601 AGAGGCCCCA GATGGCTGTC TTGCTTCCCA GACTCATGTA CCAGCCTGGT GTCCCTGAAC
          661 TTTGCCTGCA TCAAAGGCGA AGTGAATGCT GGGTCCCTGG AGCGGCTGGT CTCAAGAAGC
          721 CCCAACCTGA GGTCTCTGCG GCTGAACCGG AGCGTGAGCG TGGACACTCT GGCTAAGATT
          781 CTGCTGAGAA CCCCTAACCT GGAGGATCTG GGAACCGGCA ATCTGACAGA CGATTTCCAG
          841 ACAGAATCCT ACTTTAAACT GACTTCTGCC CTGGAGAAGT GTAAAATGCT GAGGAGTCTG
          901 TCACGATTCT GGGATGCTTC ACCCGTGTGC CCTGAGCTTT ATCTACCCTC TGTGTGCACA
          961 GCTGACAGGC CTGAAACCCT GAGCTATGCA CCAACCCTGC ACGCCCAGTG GATTCTGAAC
         1021 AAGAATGAAT CTCCACGCTT GCGTTGAAAC TCAGCGGACT GTGGATGCTG GAACCTGTAT
        1081 TTCGATAGGG CTCCCAAG
        //
        Primer: PGK_N_rev
        Region: loxP, SV40 p(A), insert 3'
        Sequence file: RDB16811_A8Lpb.seq check
        >D06157A7_A8LP_1_PGK_N_rev_E03_15_ABI24.ab1
            1 CCTGTACGCT CCTACCCGGT AGATTTCGAG GTCGAGATCC TAAGCTTGGC TGGACGTAAA
           61 CTCCTCTTCA GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT
          121 ATTGAATATG ATCGCTTAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG
          181 TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA
          241 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG
          301 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT
          361 GATCTAGAGT CGCGGCCCCG GTGGATCCCG GGCCCGCGGT ACCGTCGACG GGCCCGGGAT
          421 CCGATGGATC CTCACAGAAT CTTCACAAAG TTGGGAGCAT CATCTCTTGC CCCTGCGGTG
          481 GTCCGATACA CATACAGTTT CTCCACCTTT GGCAGGTCGC CATGATTTTC CTCCATTTCG
          541 TTAGACCCGT CCCGCTCATT AATGACTTCC ACGTTCAGCA TAGGCATTTT ACTAGCCAGG
          601 ACCTGACAGC CCTTCAGAGT CACATTGCAA CTAGACATCC ACAGGCTCCT CATTGTCTCG
          661 TATCTGGCAA AGTTTCCCAG CAGAGCGGCA TCCCCGAAAG GGCTGTCCCG GATTTCCAGT
          721 TTTCGCAGAT TCTTACACCC GTTCATCACG TGCATCATGC CCTTATCGGA GTCTCCGGCA
          781 AATGCAATGC TCAGCATCTC CAGCTGCTTA GCATACTTGC CGATGTACAT GAACACTTTG
          841 TCTGTCAGCA GGCCGGAAAT GCTCAGCCTC TGGAGTCCCT TGCACTCTCT CACGATAGCT
          901 CCAAACCCTT CATCCAGTGG CTGGGATGTG ACCACGTCGG GTTTGCCTGG TTTCCAGAAT
          961 ACACAGCCCG AAATCGGGTG AAATTTAGGG CAGTTCTTGA CGACGGTCAC CAGTGCAGCA
         1021 TTTAGGTCAT CTGGATGACC AGGAATTACA GCAAGCTGAT CCAGCTTTTG GGTCATTCCC
        1081 CAGTGAGAAC CTGCTC
        //
        Primer: PGKpro_F
        Region: PGK pro, PuroR, mouse PGK ter
        Sequence file: RDB16811_A8Lpc.seq check
        >D06157A7_A8LP_1_PGKpro_F_F03_18_ABI24.ab1
            1 AGTCGGCGCG TATCTCTCTT CTCATCTCCG GGCCTTTCGA CCTGCATCCA TCTAGATCTC
           61 GAGCAGCTGA AGCTTACCAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC
          121 GACGTCCCCA GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC
          181 CACACCGTCG ATCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC
          241 ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG
          301 GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC
          361 ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG
          421 CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC
          481 CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC
          541 GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCACAACC TCCCCTTCTA CGAGCGGCTC
          601 GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG GTGCATGACC
          661 CGCAAGCCCG GTGCCTGACG CCCGCCCCAC GACCCGCAGC GCCCGACCGA AAGGAGCGCA
          721 CGACCCCATG CATCGATGAT ATCAGATCCC CGGGATGCAG AAATTGATGA TCTATTAAAC
          781 AATAAAGATG TCCACTAAAA TGGAAGTTTT TCCTGTCATA CTTTGTTAAG AAGGGTGAGA
          841 ACAGAGTACC TACATTTTGA ATGGAAGGAT TGGAGCTACG GGGGTGGGGG TGGGGGTGGG
          901 ATTAGATAAA TGCCTGCTCT TTACTGAAGG CTCTTTACTA TTGCTTTATG ATAATGTTTC
          961 ATAGTTGGAT ATCATAATTT AAACAAGCAA AAACCCAAAT TAATGGCCAG CCTCATTTCT
         1021 TCCCACCTTC ATGATCTATA GATCCTATAG GAATCTCTTC GTTGGGATCA TTGGATTTTC
        1081 CTCATGGATT CCCACTCTTT TGCTG
        //
        Primer: reverse
        Region: T3 pro, AAVS1 arm1, CMV en
        Sequence file: RDB16811_A8Lpd.seq check
        >D06157A7_A8LP_1_reverse_G03_21_ABI24.ab1
            1 ATTAAATAAC CTCACTAAAG GGACTAGTCC TGCAGGTTTA AACGAATTCG CCCTTTGCTT
           61 TCTCTGACCA GCATTCTCTC CCCTGGGCCT GTGCCGCTTT CTGTCTGCAG CTTGTGGCCT
          121 GGGTCACCTC TACGGCTGGC CCAGATCCTT CCCTGCCGCC TCCTTCAGGT TCCGTCTTCC
          181 TCCACTCCCT CTTCCCCTTG CTCTCTGCTG TGTTGCTGCC CAAGGATGCT CTTTCCGGAG
          241 CACTTCCTTC TCGGCGCTGC ACCACGTGAT GTCCTCTGAG CGGATCCTCC CCGTGTCTGG
          301 GTCCTCTCCG GGCATCTCTC CTCCCTCACC CAACCCCATG CCGTCTTCAC TCGCTGGGTT
          361 CCCTTTTCCT TCTCCTTCTG GGGCCTGTGC CATCTCTCGT TTCTTAGGAT GGCCTTCTCC
          421 GACGGATGTC TCCCTTGCGT CCCGCCTCCC CTTCTTGTAG GCCTGCATCA TCACCGTTTT
          481 TCTGGACAAC CCCAAAGTAC CCCGTCTCCC TGGCTTTAGC CACCTCTCCA TCCTCTTGCT
          541 TTCTTTGCCT GGACACCCCG TTCTCCTGTG GATTCGGGTC ACCTCTCACT CCTTTCATTT
          601 GGGCAGCTCC CCTACCCCCC TTACCTCTCT AGTCTGTGCT AGCTCTTCCA GCCCCCTGTC
          661 ATGGCATCTT CCAGGGGTCC GAGAGCTCAG CTAGTCTTCT TCCTCCAACC CGGGCCCCTA
          721 TGTCCACTTC AGGACAGCAT GTTTGCTGCC TCCAGGGATC CTGTGTCCCC GAGCTGGGAC
          781 CACCTTATAT TCCCAGGGCC GGTTAATGTG GCTCTGGTTC TGGGTACTTT TATCTGTCCC
          841 CTCCACCCCA CAGTGGGGCA AGCTAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT
          901 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG
          961 ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGGCG ATAGGACTTT
         1021 CCCATTTGAA CGTTCATTGG ATGAGGTATT AACGGTAACT GACCCACGTT GGCCAGTTAC
        1081 CATCCAAGGG TGCTTATC
        //
        Primer: PuroR_F
        Region: PuroR, mouse PGK ter, loxP, AAVS1 arm2
        Sequence file: RDB16811_A8Lpe.seq check
        >D06157A7_A8LP_1_PuroR_F_H03_24_ABI24.ab1
            1 GCGGTCAGCC GACGTCGAGG TGCCCGAGGA CCGCGCACCT GGTGCATGAC CCGCAAGCCC
           61 GGTGCCTGAC GCCCGCCCCA CGACCCGCAG CGCCCGACCG AAAGGAGCGC ACGACCCCAT
          121 GCATCGATGA TATCAGATCC CCGGGATGCA GAAATTGATG ATCTATTAAA CAATAAAGAT
          181 GTCCACTAAA ATGGAAGTTT TTCCTGTCAT ACTTTGTTAA GAAGGGTGAG AACAGAGTAC
          241 CTACATTTTG AATGGAAGGA TTGGAGCTAC GGGGGTGGGG GTGGGGTGGG ATTAGATAAA
          301 TGCCTGCTCT TTACTGAAGG CTCTTTACTA TTGCTTTATG ATAATGTTTC ATAGTTGGAT
          361 ATCATAATTT AAACAAGCAA AACCAAATTA AGGGCCAGCT CATTCCTCCC ACTCATGATC
          421 TATAGATCTA TAGATCTCTC GTGGGATCAT TGTTTTTCTC TTGATTCCCA CTTTGTGGTT
          481 CTAAGTACTG TGGTTTCCAA ATGTGTCAGT TTCATAGCCT GAAGAACGAG ATCAGCAGCC
          541 TCTGTTCCAC ATACACTTCA TTCTCAGTAT TGTTTTGCCA AGTTCTAATT CCATCAGAAG
          601 CTGGTCGAGA TCCGGAACCC TTAATATAAC TTCGTATAAT GTATGCTATA CGAAGTTATT
          661 AGGTCCCTCG AAGAGGTTCA CTAAGCTTTA CTAGGGACAG GATTGGTGAC AGAAAAGCCC
          721 CATCCTTAGG CCTCCTCCTT CCTAGTCTCC TGATATTGGG TCTAACCCCC ACCTCCTGTT
          781 AGGCAGATTC CTTATCTGGT GACACACCCC CATTTCCTGG AGCCATCTCT CTCCTTGCCA
          841 GAACCTCTAA GGTTTGCTTA CGATGGAGCC AGAGAGGATC CTGGGAGGGA GAGCTTGGCA
          901 GGGGGTGGAG GGAAAGGGGG GGATGCGTGA CCTGCCCGGG TTCTCAGTGG CCACCCTGCG
          961 CTACCCTCTC CCAGAACCCT GAGCTGCTCT GACGCGGCTG TCTGGGTGCG TTTCACTGAA
         1021 TCCCTGATGC TGCAGCCTTC CCTTTACACT TCCTCAGGAG AGAAAGCCAG TTGGAAAACC
        1081 AAACTTCAGA TTAGTTTGGT TCCCTGAAGA C
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

        2021.11.02

        GNP_filter3_RDBDEP_html_211029.pl