Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK364 (CMV-OsTIR1-loxP-PURO-loxP)

A donor vector for introducing CMV-OsTIR1 at the human AAVS1 locus.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16811_A8Lpp1-4.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,OsTIR1 5' Sequence file: RDB16811_A8Lpf.seq
>D06157A7_A8LP_1_CMV-Forward_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNTGNN GCNNTGGGCG GTANGCGTGT ACGGTGGGNN GTCTATATAA    61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTG CCACCATGAC ATACTTTCCT   121 GAAGAGGTCG TCGAACACAT TTTTAGCTTC CTGCCTGCAC AGAGAGATAG AAACACAGTG   181 AGCCTGGTCT GCAAAGTGTG GTACGAGATC GAACGCCTGA GCCGGAGAGG AGTGTTCGTC   241 GGCAACTGCT ATGCTGTGAG AGCAGGCAGG GTCGCCGCTA GGTTTCCAAA TGTGCGCGCA   301 CTGACCGTCA AGGGGAAACC CCACTTCGCC GACTTTAACC TGGTGCCCCC TGATTGGGGA   361 GGATACGCCG GCCCTTGGAT CGAGGCAGCC GCTCGCGGCT GTCATGGACT GGAGGAACTG   421 CGCATGAAGC GAATGGTGGT CTCTGACGAA AGTCTGGAGC TGCTGGCTCG GAGCTTCCCT   481 AGGTTTCGCG CACTGGTGCT GATTTCTTGC GAAGGCTTCA GCACCGATGG ACTGGCAGCC   541 GTGGCCTCCC ACTGTAAGCT GCTGCGGGAG CTGGACCTCC AGGAGAATGA AGTGGAGGAT   601 AGAGGCCCCA GATGGCTGTC TTGCTTCCCA GACTCATGTA CCAGCCTGGT GTCCCTGAAC   661 TTTGCCTGCA TCAAAGGCGA AGTGAATGCT GGGTCCCTGG AGCGGCTGGT CTCAAGAAGC   721 CCCAACCTGA NGTCTCTGCG GCTGANNCGG ANCGTGAGCG TGGACACTCT GGCTAAGATT   781 CTGCTGANAA CNCCTAACCT GGNNGNNCTG GGAANCGGCA ATCTGACAGA CGANTTCCAG   841 ANAGANTCCT ACTTTAAANT GANNTCTGNC CNNNNNAANT GTAAAATGNT NNNNNNNCTG 901 NNNNNNNTCT GGNNNNNNTC ACCNNNNNNN CNNNANCNTN NNNNNNCNNN NNNNNNN //
Primer: PGK_N_rev (Pr0243) Region: loxP,SV40 pA,OsTIR1 3' Sequence file: RDB16811_A8Lpg.seq
>D06157A7_A8LP_1_PGK_N_rev_E03_15_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGGN ANANTTCGAG GTCGANNNCC TAAGCTTGGC TGGACGTAAA    61 CTCCTCTTCN GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT   121 ATTGAATATG ATCGCTTAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG   181 TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA   241 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG   301 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT   361 GATCTAGAGT CGCGGCCCCG GTGGATCCCG GGCCCGCGGT ACCGTCGACG GGCCCGGGAT   421 CCGATGGATC CTCACAGAAT CTTCACAAAG TTGGGAGCAT CATCTCTTGC CCCTGCGGTG   481 GTCCGATACA CATACAGTTT CTCCACCTTT GGCAGGTCGC CATGATTTTC CTCCATTTCG   541 TTAGACCCGT CCCGCTCATT AATGACTTCC ACGTTCAGCA TAGGCATTTT ACTAGCCAGG   601 ACCTGACAGC CCTTCAGAGT CACATTGCAA CTAGACATCC ACAGGCTCCT CATTGTCTCG   661 TATCTGGCAA AGTTTCCCAG CAGAGCGGCA TCCCCGAAAG GGCTGTCCCG GATTTCCAGT   721 TTTCGCAGAT TCTTACACCC GTTCATCACG TGCATCATGC CCTTATCGGA NTCTCCNGCA   781 AATGCAATGC TCANCATCTC CAGCTGCTTA NCATACTTGC CNANGTACAT GAACACNNNG   841 TCTGTCANCA NGNCNGAAAN GCTCAGCCTC NGNANNCCCT NNCANTCTNN CACGATANNN 901 CCNAANCCTN CNTNCNNNGN NTGGNNNNNN ANNNCNNNNG NNTNNNNNNN NNN //
Primer: PGKpro_F (Pr0249) Region: PuroR,mPGK ter Sequence file: RDB16811_A8Lph.seq
>D06157A7_A8LP_1_PGKpro_F_F03_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNTT NNNNNCTCCN GGCCTTTCGA CCTGCATCCN TCTAGATCTC    61 GAGCAGCTGA AGCTTACCAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC   121 GACGTCCCCA GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC   181 CACACCGTCG ATCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC   241 ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG   301 GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC   361 ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG   421 CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC   481 CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC   541 GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCACAACC TCCCCTTCTA CGAGCGGCTC   601 GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG GTGCATGACC   661 CGCAAGCCCG GTGCCTGACG CCCGCCCCAC GACCCGCAGC GCCCGACCGA AAGGAGCGCA   721 CGACCCCATG CATCGATGAT ATCAGATCCC CGGGATGCAG AAATTGATGA TCTATTAAAC   781 AATAAAGATG TCCACTNAAA TGGAAGTTTT TCCTGTCATA CTTTGTTAAG AANGGTGANA   841 ACAGANTANC TACATTTTGA ATGGANNGAN TGGNNCTANN GGGGNGGGGG NGGGGGNGGG   901 NNTAGATAAA TGNCNGNTNT TTACNNNANG NTNNTTACTA NNGNTTTNNG ANNNNNNTNN 961 NNNNNNNN //
Primer: PuroR_F (Pr0554) Region: PuroR,mPGK ter,loxP,AAVS1 arm 2 Sequence file: RDB16811_A8Lpi.seq
>D06157A7_A8LP_1_PuroR_F_H03_24_ABI24.ab1     1 NNNNNNNNNN NNNNNCNNNG NGCCCGANGN NCGCGCACCT GGTGCATGAC CCGCAAGCCC    61 GGTGCCTGAC GCCCGCCCCA CGACCCGCAG CGCCCGACCG AAAGGAGCGC ACGACCCCAT   121 GCATCGATGA TATCAGATCC CCGGGATGCA GAAATTGATG ATCTATTAAA CAATAAAGAT   181 GTCCACTAAA ATGGAAGTTT TTCCTGTCAT ACTTTGTTAA GAAGGGTGAG AACAGAGTAC   241 CTACATTTTG AATGGAAGGA TTGGAGCTAC GGGGGTGGGG GTGGGGTGGG ATTAGATAAA   301 TGCCTGCTCT TTACTGAAGG CTCTTTACTA TTGCTTTATG ATAATGTTTC ATAGTTGGAT   361 ATCATAATTT AAACAAGCAA AACCAAATTA AGGGCCAGCT CATTCCTCCC ACTCATGATC   421 TATAGATCTA TAGATCTCTC GTGGGATCAT TGTTTTTCTC TTGATTCCCA CTTTGTGGTT   481 CTAAGTACTG TGGTTTCCAA ATGTGTCAGT TTCATAGCCT GAAGAACGAG ATCAGCAGCC   541 TCTGTTCCAC ATACACTTCA TTCTCAGTAT TGTTTTGCCA AGTTCTAATT CCATCAGAAG   601 CTGGTCGAGA TCCGGAACCC TTAATATAAC TTCGTATAAT GTATGCTATA CGAAGTTATT   661 AGGTCCCTCG AAGAGGTTCA CTAAGCTTTA CTAGGGACAG GANTGGTGAC AGAAAAGCCC   721 CATCCTTANG CCTCCTCCTT CCTAGTCTCC TGATATTGGG TCTAACCCCC ACCTCCTGTT   781 ANGCAGANTC CTTATCTGGT GACACACCCC CATTTCCTGG ANCCNTCTCT CTCCTTGCCN   841 GAACCTCTAA NGTTTGCTTA CNATGGANCC NGANNNGATC CNGGGANGGA NANCTTNNNN   901 GGGGGNNNNN GGNANNGGGG GGNNGCGTGN NCTGNCCNGN TNCTCANNGN NNNNCNNNNN 961 NNNCCNNNNN CNNNNNNCNN NNNNNNNN //
Primer: pUC-CAPbs_up_F (Pr0536) Region: T3 pro,AAVS1 arm 1 Sequence file: RDB16811_A8Lpj.seq
>16811_16811_B3C2F_pUC-CAPbs_up_F_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NCNNNNNCAA TTAANGTGAG TTAGCTCACT CATTAGGCAC    61 CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC   121 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCTCAGAATT AACCCTCACT   181 AAAGGGACTA GTCCTGCAGG TTTAAACGAA TTCGCCCTTT GCTTTCTCTG ACCAGCATTC   241 TCTCCCCTGG GCCTGTGCCG CTTTCTGTCT GCAGCTTGTG GCCTGGGTCA CCTCTACGGC   301 TGGCCCAGAT CCTTCCCTGC CGCCTCCTTC AGGTTCCGTC TTCCTCCACT CCCTCTTCCC   361 CTTGCTCTCT GCTGTGTTGC TGCCCAAGGA TGCTCTTTCC GGAGCACTTC CTTCTCGGCG   421 CTGCACCACG TGATGTCCTC TGAGCGGATC CTCCCCGTGT CTGGGTCCTC TCCGGGCATC   481 TCTCCTCCCT CACCCAACCC CATGCCGTCT TCACTCGCTG GGTTCCCTTT TCCTTCTCCT   541 TCTGGGGCCT GTGCCATCTC TCGTTTCTTA GGATGGCCTT CTCCGACGGA TGTCTCCCTT   601 GCGTCCCGCC TCCCCTTCTT GTANGCCTGC ATCATCACCG TTTTTCTGGA CAACCCCAAA   661 GTACCCCGTC TCCCTGGCTT TAGCCACCTC TCCATCCTCT TGCTTTCTTT GCCTGGACAC   721 CCCGTTCTCC TGTGGATTCG GGTCACCTCT CACTCCTTTC ATTTGGGCAG CTCCCCTACC   781 CCCCTTACCT CTCTAGTCTG TGCTAGCTCT TCCAGCCCCC NGTCATGGCA TCTTCCAGGG   841 GTNCNANANC TCANCTAGTC TTCTNNCTCC NNNCNNGGGC CCCTATGNNN ACTTNNNNAN   901 AGCANGNTNG CTGCCNCCAG GGNTCCNNNN NNCNCNANNN GGNNNNNNNN TNANNNNCNN 961 NNGNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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