Resource data sheet
Prev.

pMK364 (CMV-OsTIR1-loxP-PURO-loxP)

A donor vector for introducing CMV-OsTIR1 at the human AAVS1 locus.

Catalog number RDB16811
Resource name pMK364 (CMV-OsTIR1-loxP-PURO-loxP)
Clone info. A donor vector for introducing CMV-OsTIR1 at the human AAVS1 locus. Oryza sativa OsTIR1 (NP_001052659.1), codon optimized cDNA for the expression in human cells.
Vector backbone plasmid
Selectable markers Am^r (E. coli), Puro^r (mammalian cells)
Gene/insert name human AAVS1 genomic Oryza sativa OsTIR1 cDNA
Depositor Kanemaki, Masato |
 
Other clones in our bank human AAVS1 (NCBI Gene 17) | Oryza sativa LOC4335696 |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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RDB16811 pMK364 (CMV-OsTIR1-loxP-PURO-loxP) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16811_A8Lpp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB16811_A8Lpa.seq
>D06157A7_A8LP_1_CMV-Forward_D03_12_ABI24.ab1
    1 ATGTTCCTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTG CCACCATGAC ATACTTTCCT
  121 GAAGAGGTCG TCGAACACAT TTTTAGCTTC CTGCCTGCAC AGAGAGATAG AAACACAGTG
  181 AGCCTGGTCT GCAAAGTGTG GTACGAGATC GAACGCCTGA GCCGGAGAGG AGTGTTCGTC
  241 GGCAACTGCT ATGCTGTGAG AGCAGGCAGG GTCGCCGCTA GGTTTCCAAA TGTGCGCGCA
  301 CTGACCGTCA AGGGGAAACC CCACTTCGCC GACTTTAACC TGGTGCCCCC TGATTGGGGA
  361 GGATACGCCG GCCCTTGGAT CGAGGCAGCC GCTCGCGGCT GTCATGGACT GGAGGAACTG
  421 CGCATGAAGC GAATGGTGGT CTCTGACGAA AGTCTGGAGC TGCTGGCTCG GAGCTTCCCT
  481 AGGTTTCGCG CACTGGTGCT GATTTCTTGC GAAGGCTTCA GCACCGATGG ACTGGCAGCC
  541 GTGGCCTCCC ACTGTAAGCT GCTGCGGGAG CTGGACCTCC AGGAGAATGA AGTGGAGGAT
  601 AGAGGCCCCA GATGGCTGTC TTGCTTCCCA GACTCATGTA CCAGCCTGGT GTCCCTGAAC
  661 TTTGCCTGCA TCAAAGGCGA AGTGAATGCT GGGTCCCTGG AGCGGCTGGT CTCAAGAAGC
  721 CCCAACCTGA GGTCTCTGCG GCTGAACCGG AGCGTGAGCG TGGACACTCT GGCTAAGATT
  781 CTGCTGAGAA CCCCTAACCT GGAGGATCTG GGAACCGGCA ATCTGACAGA CGATTTCCAG
  841 ACAGAATCCT ACTTTAAACT GACTTCTGCC CTGGAGAAGT GTAAAATGCT GAGGAGTCTG
  901 TCACGATTCT GGGATGCTTC ACCCGTGTGC CCTGAGCTTT ATCTACCCTC TGTGTGCACA
  961 GCTGACAGGC CTGAAACCCT GAGCTATGCA CCAACCCTGC ACGCCCAGTG GATTCTGAAC
 1021 AAGAATGAAT CTCCACGCTT GCGTTGAAAC TCAGCGGACT GTGGATGCTG GAACCTGTAT
1081 TTCGATAGGG CTCCCAAG
//
Primer: PGK_N_rev RDB16811_A8Lpb.seq
>D06157A7_A8LP_1_PGK_N_rev_E03_15_ABI24.ab1
    1 CCTGTACGCT CCTACCCGGT AGATTTCGAG GTCGAGATCC TAAGCTTGGC TGGACGTAAA
   61 CTCCTCTTCA GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT
  121 ATTGAATATG ATCGCTTAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG
  181 TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA
  241 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG
  301 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT
  361 GATCTAGAGT CGCGGCCCCG GTGGATCCCG GGCCCGCGGT ACCGTCGACG GGCCCGGGAT
  421 CCGATGGATC CTCACAGAAT CTTCACAAAG TTGGGAGCAT CATCTCTTGC CCCTGCGGTG
  481 GTCCGATACA CATACAGTTT CTCCACCTTT GGCAGGTCGC CATGATTTTC CTCCATTTCG
  541 TTAGACCCGT CCCGCTCATT AATGACTTCC ACGTTCAGCA TAGGCATTTT ACTAGCCAGG
  601 ACCTGACAGC CCTTCAGAGT CACATTGCAA CTAGACATCC ACAGGCTCCT CATTGTCTCG
  661 TATCTGGCAA AGTTTCCCAG CAGAGCGGCA TCCCCGAAAG GGCTGTCCCG GATTTCCAGT
  721 TTTCGCAGAT TCTTACACCC GTTCATCACG TGCATCATGC CCTTATCGGA GTCTCCGGCA
  781 AATGCAATGC TCAGCATCTC CAGCTGCTTA GCATACTTGC CGATGTACAT GAACACTTTG
  841 TCTGTCAGCA GGCCGGAAAT GCTCAGCCTC TGGAGTCCCT TGCACTCTCT CACGATAGCT
  901 CCAAACCCTT CATCCAGTGG CTGGGATGTG ACCACGTCGG GTTTGCCTGG TTTCCAGAAT
  961 ACACAGCCCG AAATCGGGTG AAATTTAGGG CAGTTCTTGA CGACGGTCAC CAGTGCAGCA
 1021 TTTAGGTCAT CTGGATGACC AGGAATTACA GCAAGCTGAT CCAGCTTTTG GGTCATTCCC
1081 CAGTGAGAAC CTGCTC
//
Primer: PGKpro_F RDB16811_A8Lpc.seq
>D06157A7_A8LP_1_PGKpro_F_F03_18_ABI24.ab1
    1 AGTCGGCGCG TATCTCTCTT CTCATCTCCG GGCCTTTCGA CCTGCATCCA TCTAGATCTC
   61 GAGCAGCTGA AGCTTACCAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC
  121 GACGTCCCCA GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC
  181 CACACCGTCG ATCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC
  241 ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG
  301 GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC
  361 ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG
  421 CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC
  481 CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC
  541 GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCACAACC TCCCCTTCTA CGAGCGGCTC
  601 GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG GTGCATGACC
  661 CGCAAGCCCG GTGCCTGACG CCCGCCCCAC GACCCGCAGC GCCCGACCGA AAGGAGCGCA
  721 CGACCCCATG CATCGATGAT ATCAGATCCC CGGGATGCAG AAATTGATGA TCTATTAAAC
  781 AATAAAGATG TCCACTAAAA TGGAAGTTTT TCCTGTCATA CTTTGTTAAG AAGGGTGAGA
  841 ACAGAGTACC TACATTTTGA ATGGAAGGAT TGGAGCTACG GGGGTGGGGG TGGGGGTGGG
  901 ATTAGATAAA TGCCTGCTCT TTACTGAAGG CTCTTTACTA TTGCTTTATG ATAATGTTTC
  961 ATAGTTGGAT ATCATAATTT AAACAAGCAA AAACCCAAAT TAATGGCCAG CCTCATTTCT
 1021 TCCCACCTTC ATGATCTATA GATCCTATAG GAATCTCTTC GTTGGGATCA TTGGATTTTC
1081 CTCATGGATT CCCACTCTTT TGCTG
//
Primer: reverse RDB16811_A8Lpd.seq
>D06157A7_A8LP_1_reverse_G03_21_ABI24.ab1
    1 ATTAAATAAC CTCACTAAAG GGACTAGTCC TGCAGGTTTA AACGAATTCG CCCTTTGCTT
   61 TCTCTGACCA GCATTCTCTC CCCTGGGCCT GTGCCGCTTT CTGTCTGCAG CTTGTGGCCT
  121 GGGTCACCTC TACGGCTGGC CCAGATCCTT CCCTGCCGCC TCCTTCAGGT TCCGTCTTCC
  181 TCCACTCCCT CTTCCCCTTG CTCTCTGCTG TGTTGCTGCC CAAGGATGCT CTTTCCGGAG
  241 CACTTCCTTC TCGGCGCTGC ACCACGTGAT GTCCTCTGAG CGGATCCTCC CCGTGTCTGG
  301 GTCCTCTCCG GGCATCTCTC CTCCCTCACC CAACCCCATG CCGTCTTCAC TCGCTGGGTT
  361 CCCTTTTCCT TCTCCTTCTG GGGCCTGTGC CATCTCTCGT TTCTTAGGAT GGCCTTCTCC
  421 GACGGATGTC TCCCTTGCGT CCCGCCTCCC CTTCTTGTAG GCCTGCATCA TCACCGTTTT
  481 TCTGGACAAC CCCAAAGTAC CCCGTCTCCC TGGCTTTAGC CACCTCTCCA TCCTCTTGCT
  541 TTCTTTGCCT GGACACCCCG TTCTCCTGTG GATTCGGGTC ACCTCTCACT CCTTTCATTT
  601 GGGCAGCTCC CCTACCCCCC TTACCTCTCT AGTCTGTGCT AGCTCTTCCA GCCCCCTGTC
  661 ATGGCATCTT CCAGGGGTCC GAGAGCTCAG CTAGTCTTCT TCCTCCAACC CGGGCCCCTA
  721 TGTCCACTTC AGGACAGCAT GTTTGCTGCC TCCAGGGATC CTGTGTCCCC GAGCTGGGAC
  781 CACCTTATAT TCCCAGGGCC GGTTAATGTG GCTCTGGTTC TGGGTACTTT TATCTGTCCC
  841 CTCCACCCCA CAGTGGGGCA AGCTAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT
  901 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG
  961 ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGGCG ATAGGACTTT
 1021 CCCATTTGAA CGTTCATTGG ATGAGGTATT AACGGTAACT GACCCACGTT GGCCAGTTAC
1081 CATCCAAGGG TGCTTATC
//
Primer: PuroR_F RDB16811_A8Lpe.seq
>D06157A7_A8LP_1_PuroR_F_H03_24_ABI24.ab1
    1 GCGGTCAGCC GACGTCGAGG TGCCCGAGGA CCGCGCACCT GGTGCATGAC CCGCAAGCCC
   61 GGTGCCTGAC GCCCGCCCCA CGACCCGCAG CGCCCGACCG AAAGGAGCGC ACGACCCCAT
  121 GCATCGATGA TATCAGATCC CCGGGATGCA GAAATTGATG ATCTATTAAA CAATAAAGAT
  181 GTCCACTAAA ATGGAAGTTT TTCCTGTCAT ACTTTGTTAA GAAGGGTGAG AACAGAGTAC
  241 CTACATTTTG AATGGAAGGA TTGGAGCTAC GGGGGTGGGG GTGGGGTGGG ATTAGATAAA
  301 TGCCTGCTCT TTACTGAAGG CTCTTTACTA TTGCTTTATG ATAATGTTTC ATAGTTGGAT
  361 ATCATAATTT AAACAAGCAA AACCAAATTA AGGGCCAGCT CATTCCTCCC ACTCATGATC
  421 TATAGATCTA TAGATCTCTC GTGGGATCAT TGTTTTTCTC TTGATTCCCA CTTTGTGGTT
  481 CTAAGTACTG TGGTTTCCAA ATGTGTCAGT TTCATAGCCT GAAGAACGAG ATCAGCAGCC
  541 TCTGTTCCAC ATACACTTCA TTCTCAGTAT TGTTTTGCCA AGTTCTAATT CCATCAGAAG
  601 CTGGTCGAGA TCCGGAACCC TTAATATAAC TTCGTATAAT GTATGCTATA CGAAGTTATT
  661 AGGTCCCTCG AAGAGGTTCA CTAAGCTTTA CTAGGGACAG GATTGGTGAC AGAAAAGCCC
  721 CATCCTTAGG CCTCCTCCTT CCTAGTCTCC TGATATTGGG TCTAACCCCC ACCTCCTGTT
  781 AGGCAGATTC CTTATCTGGT GACACACCCC CATTTCCTGG AGCCATCTCT CTCCTTGCCA
  841 GAACCTCTAA GGTTTGCTTA CGATGGAGCC AGAGAGGATC CTGGGAGGGA GAGCTTGGCA
  901 GGGGGTGGAG GGAAAGGGGG GGATGCGTGA CCTGCCCGGG TTCTCAGTGG CCACCCTGCG
  961 CTACCCTCTC CCAGAACCCT GAGCTGCTCT GACGCGGCTG TCTGGGTGCG TTTCACTGAA
 1021 TCCCTGATGC TGCAGCCTTC CCTTTACACT TCCTCAGGAG AGAAAGCCAG TTGGAAAACC
1081 AAACTTCAGA TTAGTTTGGT TCCCTGAAGA C
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

2019.07.18

GNP_filter3_RDBDEP_html_190432.pl