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pMK401 (Hygro-P2A-mAID-Nluc)

A plasmid for construction of N-terminal Hygro-P2A-mAID-Nluc tagging donors.

Catalog number RDB16823
Resource name pMK401 (Hygro-P2A-mAID-Nluc)
Clone info. A plasmid for construction of N-terminal Hygro-P2A-mAID-Nluc tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Oplophorus gracilirostris luciferase cDNA
Depositor Kanemaki, Masato |
 
Other clones in our bank Arabidopsis thaliana AXR3 (KEGG orthology K14484) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB16823 pMK401 (Hygro-P2A-mAID-Nluc) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16823_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 RDB16823_A8Lpa.seq
>D06157C1_A8Lp_1_pUC-M13_-100_D08_11_ABI24.ab1
    1 GGTCGGTCCA GCCGGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTTGTA
   61 AAACGACGGC CAGTGAGCGC GCGTAATACG ACTCACTATA GGGCGAATTG GGTACCGGGC
  121 CCCCCCTCGA GGTCGACGGT ATCGATAAGC TTGATATCGC CACCATGAAA AAGCCTGAAC
  181 TCACCGCGAC GTCTGTCGAG AAGTTTCTGA TCGAAAAGTT CGACAGCGTC TCCGACCTGA
  241 TGCAGCTCTC GGAGGGCGAA GAATCTCGTG CTTTCAGCTT CGATGTAGGA GGGCGTGGAT
  301 ATGTCCTGCG GGTAAATAGC TGCGCCGATG GTTTCTACAA AGATCGTTAT GTTTATCGGC
  361 ACTTTGCATC GGCCGCGCTC CCGATTCCGG AAGTGCTTGA CATTGGGGAA TTCAGCGAGA
  421 GCCTGACCTA TTGCATCTCC CGCCGTGCAC AGGGTGTCAC GTTGCAAGAC CTGCCTGAAA
  481 CCGAACTGCC CGCTGTTCTG CAGCCGGTCG CGGAGGCCAT GGATGCGATC GCTGCGGCCG
  541 ATCTTAGCCA GACGAGCGGG TTCGGCCCAT TCGGACCGCA AGGAATCGGT CAATACACTA
  601 CATGGCGTGA TTTCATATGC GCGATTGCTG ATCCCCATGT GTATCACTGG CAAACTGTGA
  661 TGGACGACAC CGTCAGTGCG TCCGTCGCGC AGGCTCTCGA TGAGCTGATG CTTTGGGCCG
  721 AGGACTGCCC CGAAGTCCGG CACCTCGTGC ACGCGGATTT CGGCTCCAAC AATGTCCTGA
  781 CGGACAATGG CCGCATAACA GCGGTCATTG ACTGGAGCGA GGCGATGTTC GGGGATTCCC
  841 AATACGAGGT CGCCAACATC TTCTTCTGGA GGCCGTGGTT GGCTTGTATG GAGCAGCAGA
  901 CGCGCTACTT CGAGCGGGAG GCATCCGGAG CTTGCAGATC GCCGCGGCTC CCGGGCGTAT
  961 ATGCTCCGCA TTGGTCTTGA CCAACTTCTT ATCAGAGCTT GGTTTGACGG CCAATTTTCG
 1021 ATGGATGCAG CTTTGGCGCA AGGTTCGATG CGACGCAATC GGTTCCGATT CGGAGCCGGA
1081 ACTTGCTCGG GGCGTTACCA CAAATTCGTC CCCG
//
Primer: reverse RDB16823_A8Lpb.seq
>D06157C1_A8Lp_1_reverse_E08_14_ABI24.ab1
    1 CCCGCCAATT ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCACCGCGG TGGCGGCCGC
   61 TCTAGAACTA GTGGATCCGG CGCCGGCCCC TGCACCAGCG CCCGCCAGAA TGCGTTCGCA
  121 CAGCCGCCAG CCGGTCACTC CGTTGATGGT TACTCGGAAC AGCAGGGAGC CGTCGGGGTT
  181 GATCAGGCGC TCGTCGATAA TTTTGTTGCC GTTCCACAGG GTCCCTGTTA CAGTGATCTT
  241 TTTGCCGTCG AACACGGCGA TGCCTTCATA CGGCCGTCCG AAATAGTCGA TCATGTTCGG
  301 CGTAACCCCG TCGATTACCA GTGTGCCATA GTGCAGGATC ACCTTAAAGT GATGATCATC
  361 CACAGGGTAC ACCACCTTAA AAATTTTTTC GATCTGGCCC ATTTGGTCGC CGCTCAGACC
  421 TTCATACGGG ATGATGACAT GGATGTCGAT CTTCAGCCCA TTTTCACCGC TCAGGACAAT
  481 CCTTTGGATC GGAGTTACGG ACACCCCGAG ATTCTGAAAC AAACTGGACA CACCTCCCTG
  541 TTCAAGGACT TGGTCCAGGT TGTAGCCGGC TGTCTGTCGC CAGTCCCCAA CGAAATCTTC
  601 GAGTGTGAAG ACCATGCTAG CTTTATACAT CCTCAAATCG ATTTTCCTCA AGTACGGTGC
  661 TCCGTCCATT GATACCTTCA CGAACGCCGC CGCCTCCGGG CCACCGCTTG ATTTTTGGCA
  721 GGAAACCATC ACGTTCTTCC GGTATGATCT CACCGGTGGC CATCCCACAA CTTGTGCCTT
  781 GGCCGGAGGT TTGGCTGGAT CTTTAGGACA AGCACTCTTC TCCTTAGATC TAGGTCCAGG
  841 GTTCTCCTCC ACGTCTCCAG CCTGCTTCAG CAGCTGAAGT TAGTAGCTCC GCTTCCTGAT
  901 TCCTTTGCCC TCGGGACGAG TGCTGGGGGC GTCGGTTTCC ACTATCGGGC GAGTACTTCT
  961 ACACAGCCCA TCGGGTCCAG ACGGCCCGCG CCTTCTGCAG GCGATTTGTG TTACGCCCGG
 1021 ACAGTCCCCG GCTCCGGATT CGGACGAATT GCGTCGCAAT CGACCTTGCG CCTAAGCCTG
1081 CATCAATCGA AATTTGTCCC GGTA
//
Primer: HygR-C_F RDB16823_A8Lpc.seq
>D06157C1_A8Lp_1_HygR-C_F_F08_17_ABI24.ab1
    1 CGTACTCGGG GATCCAATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT
   61 GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC
  121 GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT CTATCAGAGC TTGGTTGACG
  181 GCAATTTCGA TGATGCAGCT TGGGCGCAGG GTCGATGCGA CGCAATCGTC CGATCCGGAG
  241 CCGGGACTGT CGGGCGTACA CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT
  301 GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG
  361 AATCAGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGAGAC GTGGAGGAGA
  421 ACCCTGGACC TAGATCTAAG GAGAAGAGTG CTTGTCCTAA AGATCCAGCC AAACCTCCGG
  481 CCAAGGCACA AGTTGTGGGA TGGCCACCGG TGAGATCATA CCGGAAGAAC GTGATGGTTT
  541 CCTGCCAAAA ATCAAGCGGT GGCCCGGAGG CGGCGGCGTT CGTGAAGGTA TCAATGGACG
  601 GAGCACCGTA CTTGAGGAAA ATCGATTTGA GGATGTATAA AGCTAGCATG GTCTTCACAC
  661 TCGAAGATTT CGTTGGGGAC TGGCGACAGA CAGCCGGCTA CAACCTGGAC CAAGTCCTTG
  721 AACAGGGAGG TGTGTCCAGT TTGTTTCAGA ATCTCGGGGT GTCCGTAACT CCGATCCAAA
  781 GGATTGTCCT GAGCGGTGAA AATGGGCTGA AGATCGACAT CCATGTCATC ATCCCGTATG
  841 AAGGTCTGAG CGGCGACCAA ATGGGCCAGA TCGAAAAAAA TTTTTTAAGG TGGTGTACCC
  901 TGTGGATGAT CATCACTTTA AGTGATCCTG CACTATGGCA CACTGGTAAT CGACGGGGGT
  961 TTACGCCGAA ACATGATCGA CTATTTCGGA CGGCCCGTAT GAACGCAATC GCGTGTTTCG
 1021 ACGGCAAAAG ATCACTGTAA CCAAGGAACC CTGTGCAACG CCAACCAAAA TTTATTCGGA
1081 ACGAGAGGCG GCT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

2019.07.12

GNP_filter3_RDBDEP_html_190431.pl