RDB16823 - pMK401 (Hygro-P2A-mAID-Nluc)

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pMK401 (Hygro-P2A-mAID-Nluc) (#RDB16823)

A plasmid for construction of N-terminal Hygro-P2A-mAID-Nluc tagging donors.

Clone info.	A plasmid for construction of N-terminal Hygro-P2A-mAID-Nluc tagging donors.
Vector backbone	pBluescript (plasmid)
Size of vector backbone	2.9 kb
Selectable markers	Am^r (E. coli)
Gene/insert name	Arabidopsis thaliana AtIAA17 cDNA Oplophorus gracilirostris luciferase cDNA
Depositor\|Developer	Kanemaki, Masato \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB16823	pMK401 (Hygro-P2A-mAID-Nluc)	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK401 (Hygro-P2A-mAID-Nluc) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16823).

Reference section:

Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB16823_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 () Sequence file: RDB16823_A8Lpa.seq
>D06157C1_A8Lp_1_pUC-M13_-100_D08_11_ABI24.ab1 1 GGTCGGTCCA GCCGGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTTGTA 61 AAACGACGGC CAGTGAGCGC GCGTAATACG ACTCACTATA GGGCGAATTG GGTACCGGGC 121 CCCCCCTCGA GGTCGACGGT ATCGATAAGC TTGATATCGC CACCATGAAA AAGCCTGAAC 181 TCACCGCGAC GTCTGTCGAG AAGTTTCTGA TCGAAAAGTT CGACAGCGTC TCCGACCTGA 241 TGCAGCTCTC GGAGGGCGAA GAATCTCGTG CTTTCAGCTT CGATGTAGGA GGGCGTGGAT 301 ATGTCCTGCG GGTAAATAGC TGCGCCGATG GTTTCTACAA AGATCGTTAT GTTTATCGGC 361 ACTTTGCATC GGCCGCGCTC CCGATTCCGG AAGTGCTTGA CATTGGGGAA TTCAGCGAGA 421 GCCTGACCTA TTGCATCTCC CGCCGTGCAC AGGGTGTCAC GTTGCAAGAC CTGCCTGAAA 481 CCGAACTGCC CGCTGTTCTG CAGCCGGTCG CGGAGGCCAT GGATGCGATC GCTGCGGCCG 541 ATCTTAGCCA GACGAGCGGG TTCGGCCCAT TCGGACCGCA AGGAATCGGT CAATACACTA 601 CATGGCGTGA TTTCATATGC GCGATTGCTG ATCCCCATGT GTATCACTGG CAAACTGTGA 661 TGGACGACAC CGTCAGTGCG TCCGTCGCGC AGGCTCTCGA TGAGCTGATG CTTTGGGCCG 721 AGGACTGCCC CGAAGTCCGG CACCTCGTGC ACGCGGATTT CGGCTCCAAC AATGTCCTGA 781 CGGACAATGG CCGCATAACA GCGGTCATTG ACTGGAGCGA GGCGATGTTC GGGGATTCCC 841 AATACGAGGT CGCCAACATC TTCTTCTGGA GGCCGTGGTT GGCTTGTATG GAGCAGCAGA 901 CGCGCTACTT CGAGCGGGAG GCATCCGGAG CTTGCAGATC GCCGCGGCTC CCGGGCGTAT 961 ATGCTCCGCA TTGGTCTTGA CCAACTTCTT ATCAGAGCTT GGTTTGACGG CCAATTTTCG 1021 ATGGATGCAG CTTTGGCGCA AGGTTCGATG CGACGCAATC GGTTCCGATT CGGAGCCGGA 1081 ACTTGCTCGG GGCGTTACCA CAAATTCGTC CCCG //
Primer: reverse () Sequence file: RDB16823_A8Lpb.seq
>D06157C1_A8Lp_1_reverse_E08_14_ABI24.ab1 1 CCCGCCAATT ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCACCGCGG TGGCGGCCGC 61 TCTAGAACTA GTGGATCCGG CGCCGGCCCC TGCACCAGCG CCCGCCAGAA TGCGTTCGCA 121 CAGCCGCCAG CCGGTCACTC CGTTGATGGT TACTCGGAAC AGCAGGGAGC CGTCGGGGTT 181 GATCAGGCGC TCGTCGATAA TTTTGTTGCC GTTCCACAGG GTCCCTGTTA CAGTGATCTT 241 TTTGCCGTCG AACACGGCGA TGCCTTCATA CGGCCGTCCG AAATAGTCGA TCATGTTCGG 301 CGTAACCCCG TCGATTACCA GTGTGCCATA GTGCAGGATC ACCTTAAAGT GATGATCATC 361 CACAGGGTAC ACCACCTTAA AAATTTTTTC GATCTGGCCC ATTTGGTCGC CGCTCAGACC 421 TTCATACGGG ATGATGACAT GGATGTCGAT CTTCAGCCCA TTTTCACCGC TCAGGACAAT 481 CCTTTGGATC GGAGTTACGG ACACCCCGAG ATTCTGAAAC AAACTGGACA CACCTCCCTG 541 TTCAAGGACT TGGTCCAGGT TGTAGCCGGC TGTCTGTCGC CAGTCCCCAA CGAAATCTTC 601 GAGTGTGAAG ACCATGCTAG CTTTATACAT CCTCAAATCG ATTTTCCTCA AGTACGGTGC 661 TCCGTCCATT GATACCTTCA CGAACGCCGC CGCCTCCGGG CCACCGCTTG ATTTTTGGCA 721 GGAAACCATC ACGTTCTTCC GGTATGATCT CACCGGTGGC CATCCCACAA CTTGTGCCTT 781 GGCCGGAGGT TTGGCTGGAT CTTTAGGACA AGCACTCTTC TCCTTAGATC TAGGTCCAGG 841 GTTCTCCTCC ACGTCTCCAG CCTGCTTCAG CAGCTGAAGT TAGTAGCTCC GCTTCCTGAT 901 TCCTTTGCCC TCGGGACGAG TGCTGGGGGC GTCGGTTTCC ACTATCGGGC GAGTACTTCT 961 ACACAGCCCA TCGGGTCCAG ACGGCCCGCG CCTTCTGCAG GCGATTTGTG TTACGCCCGG 1021 ACAGTCCCCG GCTCCGGATT CGGACGAATT GCGTCGCAAT CGACCTTGCG CCTAAGCCTG 1081 CATCAATCGA AATTTGTCCC GGTA //
Primer: HygR-C_F () Sequence file: RDB16823_A8Lpc.seq
>D06157C1_A8Lp_1_HygR-C_F_F08_17_ABI24.ab1 1 CGTACTCGGG GATCCAATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT 61 GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC 121 GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT CTATCAGAGC TTGGTTGACG 181 GCAATTTCGA TGATGCAGCT TGGGCGCAGG GTCGATGCGA CGCAATCGTC CGATCCGGAG 241 CCGGGACTGT CGGGCGTACA CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT 301 GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG 361 AATCAGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGAGAC GTGGAGGAGA 421 ACCCTGGACC TAGATCTAAG GAGAAGAGTG CTTGTCCTAA AGATCCAGCC AAACCTCCGG 481 CCAAGGCACA AGTTGTGGGA TGGCCACCGG TGAGATCATA CCGGAAGAAC GTGATGGTTT 541 CCTGCCAAAA ATCAAGCGGT GGCCCGGAGG CGGCGGCGTT CGTGAAGGTA TCAATGGACG 601 GAGCACCGTA CTTGAGGAAA ATCGATTTGA GGATGTATAA AGCTAGCATG GTCTTCACAC 661 TCGAAGATTT CGTTGGGGAC TGGCGACAGA CAGCCGGCTA CAACCTGGAC CAAGTCCTTG 721 AACAGGGAGG TGTGTCCAGT TTGTTTCAGA ATCTCGGGGT GTCCGTAACT CCGATCCAAA 781 GGATTGTCCT GAGCGGTGAA AATGGGCTGA AGATCGACAT CCATGTCATC ATCCCGTATG 841 AAGGTCTGAG CGGCGACCAA ATGGGCCAGA TCGAAAAAAA TTTTTTAAGG TGGTGTACCC 901 TGTGGATGAT CATCACTTTA AGTGATCCTG CACTATGGCA CACTGGTAAT CGACGGGGGT 961 TTACGCCGAA ACATGATCGA CTATTTCGGA CGGCCCGTAT GAACGCAATC GCGTGTTTCG 1021 ACGGCAAAAG ATCACTGTAA CCAAGGAACC CTGTGCAACG CCAACCAAAA TTTATTCGGA 1081 ACGAGAGGCG GCT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]