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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK401 (Hygro-P2A-mAID-Nluc)

A plasmid for construction of N-terminal Hygro-P2A-mAID-Nluc tagging donors.

Catalog number RDB16823
Resource name pMK401 (Hygro-P2A-mAID-Nluc)
Clone info. A plasmid for construction of N-terminal Hygro-P2A-mAID-Nluc tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Oplophorus gracilirostris luciferase cDNA
Depositor|Developer Kanemaki, Masato |
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External Database
Arabidopsis thaliana AtIAA17 Oplophorus gracilirostris luciferase

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16823 pMK401 (Hygro-P2A-mAID-Nluc) DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16823_A8Lpp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-M13_-100
          Sequence file: RDB16823_A8Lpa.seq check
          >D06157C1_A8Lp_1_pUC-M13_-100_D08_11_ABI24.ab1
              1 GGTCGGTCCA GCCGGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTTGTA
             61 AAACGACGGC CAGTGAGCGC GCGTAATACG ACTCACTATA GGGCGAATTG GGTACCGGGC
            121 CCCCCCTCGA GGTCGACGGT ATCGATAAGC TTGATATCGC CACCATGAAA AAGCCTGAAC
            181 TCACCGCGAC GTCTGTCGAG AAGTTTCTGA TCGAAAAGTT CGACAGCGTC TCCGACCTGA
            241 TGCAGCTCTC GGAGGGCGAA GAATCTCGTG CTTTCAGCTT CGATGTAGGA GGGCGTGGAT
            301 ATGTCCTGCG GGTAAATAGC TGCGCCGATG GTTTCTACAA AGATCGTTAT GTTTATCGGC
            361 ACTTTGCATC GGCCGCGCTC CCGATTCCGG AAGTGCTTGA CATTGGGGAA TTCAGCGAGA
            421 GCCTGACCTA TTGCATCTCC CGCCGTGCAC AGGGTGTCAC GTTGCAAGAC CTGCCTGAAA
            481 CCGAACTGCC CGCTGTTCTG CAGCCGGTCG CGGAGGCCAT GGATGCGATC GCTGCGGCCG
            541 ATCTTAGCCA GACGAGCGGG TTCGGCCCAT TCGGACCGCA AGGAATCGGT CAATACACTA
            601 CATGGCGTGA TTTCATATGC GCGATTGCTG ATCCCCATGT GTATCACTGG CAAACTGTGA
            661 TGGACGACAC CGTCAGTGCG TCCGTCGCGC AGGCTCTCGA TGAGCTGATG CTTTGGGCCG
            721 AGGACTGCCC CGAAGTCCGG CACCTCGTGC ACGCGGATTT CGGCTCCAAC AATGTCCTGA
            781 CGGACAATGG CCGCATAACA GCGGTCATTG ACTGGAGCGA GGCGATGTTC GGGGATTCCC
            841 AATACGAGGT CGCCAACATC TTCTTCTGGA GGCCGTGGTT GGCTTGTATG GAGCAGCAGA
            901 CGCGCTACTT CGAGCGGGAG GCATCCGGAG CTTGCAGATC GCCGCGGCTC CCGGGCGTAT
            961 ATGCTCCGCA TTGGTCTTGA CCAACTTCTT ATCAGAGCTT GGTTTGACGG CCAATTTTCG
           1021 ATGGATGCAG CTTTGGCGCA AGGTTCGATG CGACGCAATC GGTTCCGATT CGGAGCCGGA
          1081 ACTTGCTCGG GGCGTTACCA CAAATTCGTC CCCG
          //
          Primer: reverse
          Sequence file: RDB16823_A8Lpb.seq check
          >D06157C1_A8Lp_1_reverse_E08_14_ABI24.ab1
              1 CCCGCCAATT ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCACCGCGG TGGCGGCCGC
             61 TCTAGAACTA GTGGATCCGG CGCCGGCCCC TGCACCAGCG CCCGCCAGAA TGCGTTCGCA
            121 CAGCCGCCAG CCGGTCACTC CGTTGATGGT TACTCGGAAC AGCAGGGAGC CGTCGGGGTT
            181 GATCAGGCGC TCGTCGATAA TTTTGTTGCC GTTCCACAGG GTCCCTGTTA CAGTGATCTT
            241 TTTGCCGTCG AACACGGCGA TGCCTTCATA CGGCCGTCCG AAATAGTCGA TCATGTTCGG
            301 CGTAACCCCG TCGATTACCA GTGTGCCATA GTGCAGGATC ACCTTAAAGT GATGATCATC
            361 CACAGGGTAC ACCACCTTAA AAATTTTTTC GATCTGGCCC ATTTGGTCGC CGCTCAGACC
            421 TTCATACGGG ATGATGACAT GGATGTCGAT CTTCAGCCCA TTTTCACCGC TCAGGACAAT
            481 CCTTTGGATC GGAGTTACGG ACACCCCGAG ATTCTGAAAC AAACTGGACA CACCTCCCTG
            541 TTCAAGGACT TGGTCCAGGT TGTAGCCGGC TGTCTGTCGC CAGTCCCCAA CGAAATCTTC
            601 GAGTGTGAAG ACCATGCTAG CTTTATACAT CCTCAAATCG ATTTTCCTCA AGTACGGTGC
            661 TCCGTCCATT GATACCTTCA CGAACGCCGC CGCCTCCGGG CCACCGCTTG ATTTTTGGCA
            721 GGAAACCATC ACGTTCTTCC GGTATGATCT CACCGGTGGC CATCCCACAA CTTGTGCCTT
            781 GGCCGGAGGT TTGGCTGGAT CTTTAGGACA AGCACTCTTC TCCTTAGATC TAGGTCCAGG
            841 GTTCTCCTCC ACGTCTCCAG CCTGCTTCAG CAGCTGAAGT TAGTAGCTCC GCTTCCTGAT
            901 TCCTTTGCCC TCGGGACGAG TGCTGGGGGC GTCGGTTTCC ACTATCGGGC GAGTACTTCT
            961 ACACAGCCCA TCGGGTCCAG ACGGCCCGCG CCTTCTGCAG GCGATTTGTG TTACGCCCGG
           1021 ACAGTCCCCG GCTCCGGATT CGGACGAATT GCGTCGCAAT CGACCTTGCG CCTAAGCCTG
          1081 CATCAATCGA AATTTGTCCC GGTA
          //
          Primer: HygR-C_F
          Sequence file: RDB16823_A8Lpc.seq check
          >D06157C1_A8Lp_1_HygR-C_F_F08_17_ABI24.ab1
              1 CGTACTCGGG GATCCAATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT
             61 GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC
            121 GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT CTATCAGAGC TTGGTTGACG
            181 GCAATTTCGA TGATGCAGCT TGGGCGCAGG GTCGATGCGA CGCAATCGTC CGATCCGGAG
            241 CCGGGACTGT CGGGCGTACA CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT
            301 GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG
            361 AATCAGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGAGAC GTGGAGGAGA
            421 ACCCTGGACC TAGATCTAAG GAGAAGAGTG CTTGTCCTAA AGATCCAGCC AAACCTCCGG
            481 CCAAGGCACA AGTTGTGGGA TGGCCACCGG TGAGATCATA CCGGAAGAAC GTGATGGTTT
            541 CCTGCCAAAA ATCAAGCGGT GGCCCGGAGG CGGCGGCGTT CGTGAAGGTA TCAATGGACG
            601 GAGCACCGTA CTTGAGGAAA ATCGATTTGA GGATGTATAA AGCTAGCATG GTCTTCACAC
            661 TCGAAGATTT CGTTGGGGAC TGGCGACAGA CAGCCGGCTA CAACCTGGAC CAAGTCCTTG
            721 AACAGGGAGG TGTGTCCAGT TTGTTTCAGA ATCTCGGGGT GTCCGTAACT CCGATCCAAA
            781 GGATTGTCCT GAGCGGTGAA AATGGGCTGA AGATCGACAT CCATGTCATC ATCCCGTATG
            841 AAGGTCTGAG CGGCGACCAA ATGGGCCAGA TCGAAAAAAA TTTTTTAAGG TGGTGTACCC
            901 TGTGGATGAT CATCACTTTA AGTGATCCTG CACTATGGCA CACTGGTAAT CGACGGGGGT
            961 TTACGCCGAA ACATGATCGA CTATTTCGGA CGGCCCGTAT GAACGCAATC GCGTGTTTCG
           1021 ACGGCAAAAG ATCACTGTAA CCAAGGAACC CTGTGCAACG CCAACCAAAA TTTATTCGGA
          1081 ACGAGAGGCG GCT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          2021.11.02

          GNP_filter3_RDBDEP_html_211029.pl