A plasmid for construction of C-terminal mCherry tagging donors.
Alternative name | mCherry2-Hygro, 975 |
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Clone info. | A plasmid for construction of C-terminal mCherry tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone. |
Vector backbone | pBluescriptII KS- (Plasmid) |
Size of vector backbone | 3.0 kb |
Selectable markers | Am^r |
Gene/insert name | Discosoma sp. RFP cDNA |
Depositor|Developer | Kanemaki, Masato | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13924 | pMK281 (mCherry2-Hygro) | DNA solution |
Materials & Methods section:
The pMK281 (mCherry2-Hygro) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13924). |
Reference section:
Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13924_A6B8p1-3.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: M13_-40 (Pr0410) Region: T7 pro,insert 5'(Linker,mCherry2 5') Sequence file: RDB13924_A6B8e.seq |
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>D03070A08_A6B8_1_M13(-40)_E02_14.ab1 1 NNNNNNNNNN NNNCNNNNNN GNNTNNCNAT AGGGCGAATT GGANCTCGGA TCCGGTGCAG 61 GCGCCGCTAG CATGGTGAGC AAGGGCGAGG AGGATAACAT GGCCATCATC AAGGAGTTCA 121 TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG ATCGAGGGCG 181 AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG ACCAAGGGTG 241 GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CATGTACGGC TCCAAGGCCT 301 ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC GAGGGCTTCA 361 ATTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC CAGGACTCCT 421 CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC TTCCCCTCCG 481 ACGGCCCCGT AATGCAGTGT CGTACCATGG GCTGGGAGGC CTCCACTGAG CGGATGTACC 541 CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGCAGAGGCT GAAGCTGAAG GACGGCGGCC 601 ACTACGACGC TGAGGTCAAG ACCACCTACA AGGCCAAGAA GCCCGTGCAG CTGCCCGGCG 661 CCTACAACGT CGACATCAAG TTGGACATCC TTTCCCACAA CGAGGNCTAC ACCATCGTGG 721 AACAGTACGA ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAN CTGTACAAGT 781 AATCTAGATA ACTGATCATA ATCAGCCATA CCACATTTGT AGAGGTTTTA CTTGCTTTNA 841 AAAANCTCCC ACACCNCCCC CNGANNCTGA AACATAAAAN GAATGCNATT GTTGTTGNTA 901 ACTTGTTNAT TGCAGCTTNN AATGNTTACN AATAAANCAA TAGCNTCNCN ANNTTCACAA 961 ATAAAGCATT TTTTTCNCTG NNNTCTANNN GNGGNTNGNN CNAANNCATN NNNGTATNNN 1021 NNNNNNN // |
Primer: M13R (Pr0160) Region: insert 3'(loxP,mPGK ter,HygR) Sequence file: RDB13924_A6B8f.seq |
>D03070A08_A6B8_1_M13R_E01_13.ab1 1 NNNNNNNNNN NNNNNNNNNN NANGNNNNNN AAGCTGGGTA NCGGATCCTA GTGAACCTCT 61 TCGAGGGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATATTA AGGGTTCCGG 121 ATCTCGACCA GCTTCTGATG GAATTAGAAC TTGGCAAAAC AATACTGAGA ATGAAGTGTA 181 TGTGGAACAG AGGCTGCTGA TCTCGTTCTT CAGGCTATGA AACTGACACA TTTGGAAACC 241 ACAGTACTTA GAACCACAAA GTGGGAATCA AGAGAAAAAC AATGATCCCA CGAGAGATCT 301 ATAGATCTAT AGATCATGAG TGGGAGGAAT GAGCTGGCCC TTAATTTGGT TTTGCTTGTT 361 TAAATTATGA TATCCAACTA TGAAACATTA TCATAAAGCA ATAGTAAAGA GCCTTCAGTA 421 AAGAGCAGGC ATTTATCTAA TCCCACCCCA CCCCCACCCC CGTAGCTCCA ATCCTTCCAT 481 TCAAAATGTA GGTACTCTGT TCTCACCCTT CTTAACAAAG TATGACAGGA AAAACTTCCA 541 TTTTAGTGGA CATCTTTATT GTTTAATAGA TCATCAATTT CTGCATCCCG GGGATCTGAT 601 ATCATCGATG CATGGGGTCG TGCGCTCCTT TCGGTCGGGC GCTGCGGGTC GTGGGGCGGG 661 CGGCTAGCCT ATTCCTTTGC CCTCGGACGA GTGCTGGGGC GTCGGTTTCC ACTATCGGCG 721 AGTACTTCTA CACAGCCATC GGTCCAGACN GCCGCGCTTC TGCGGGCGAT TTGTGTACGC 781 CCGACAGTCC CGGCTCCNGA TCGGANGATT GCGTCGCATC GACCCTGCGC CCAAGCTGNN 841 NTCATCGAAN NTGCCGTCNA CCAANNCTCT GANAGAGTTG GTCNNGANCA ATGCNGNANC 901 ATATACGCCC NGNANNCNCN GNNATCCTGC NANCNNNNGA TGNCNNNCNC TCNNAANNNN 961 NNCGTCTGNN GCNNCNNNNN ANNNCNNANC NNNNGNNCNN NCNNNNNNNA NNNNGNNNNN 1021 NNNNN // |
Primer: PGK_N_rev (Pr0243) Region: insert mid(loxP,SV40 pA,mCherry2 3') Sequence file: RDB13924_A6B8g.seq |
>D03070A08_A6B8_1_PGK-N-rev_C06_09.ab1 1 NNNNNNNNNN NNNNNNNNNC NGGNANANTT CGAGGTCGAG ATCCTAAGCT TGGCTGGACG 61 TAAACTCCTC TTCAGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG 121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA 181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA 241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG 301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG 361 CTGATTATGA TCAGTTATCT AGATTACTTG TACAGCTCGT CCATGCCGCC GGTGGAGTGG 421 CGGCCCTCGG CGCGTTCGTA CTGTTCCACG ATGGTGTAGT CCTCGTTGTG GGAAAGGATG 481 TCCAACTTGA TGTCGACGTT GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT GGCCTTGTAG 541 GTGGTCTTGA CCTCAGCGTC GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT CTGCTTGATC 601 TCGCCCTTCA GGGCGCCGTC CTCGGGNNNN NNCCGCTCAG TGGAGGCCTC CCAGCCCATG 661 GTACGACACT GCATTACGGG GCCGTCGGAN GGGAAGTTGG TGCCNCGCAN CTTCACCTTG 721 TAGATGAACT CNNCGTCCTG CANGGANGAN NNNNTGGGTC ACGGTCACCN NNNCNCCGTC 781 CTCNAAGTTC NTCNCGCGCT CCCNNNNNNN NNCCCTCNGG GGAAGGACAG CTTCANNNNN 841 NNNNN // |
Primer: PGKpro_F (Pr0249) Region: insert mid(HygR) Sequence file: RDB13924_A6B8h.seq |
>D03070A08_A6B8_1_PGK-proF_C07_07.ab1 1 NNNNNNNNNN NNNNNNNNNN NTCTCCNGGC CTTTCGACCT GCATCCNTCT AGATCTCGAG 61 CAGCTGAAGC TTACCGCTAG CATGGATAGA TCCGGAAAGC CTGAACTCAC CGCGACGTCT 121 GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA GCTCTCGGAG 181 GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA 241 AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC 301 GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT GACCTATTGC 361 ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT 421 GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG 481 AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC 541 ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC 601 AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA 661 GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA CAATGGCCGC 721 ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA CGAGGTCGCC 781 AACATCTTCT TCTGGANGCC GTGGTTGGCT TGTATGGAGC AGCAGANGCG CTACTTCGAG 841 CGGANGCATC CGGANCTTGC NNGATCGCCG CGGCNNCNGG CGTATATGCT CCGCATTGGT 901 CTTGACNNAC TCTATCANAN CTNNNNTGAN NGCNATTTCN ATGANGCAGC TNNNNNCNNG 961 NTCGATGCNA CNNNNNCGTC CNATNNNGNA NNCNNGNNNN GNCNGGNNGT ACNNNANNNN 1021 NNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP] |
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reference | Natsume, T., Acute inactivation of the replicative helicase in human cells triggers MCM8-9-dependent DNA synthesis. Genes Dev. (2017). PMID 28487407. |
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