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pMK281 (mCherry2-Hygro)

A plasmid for construction of C-terminal mCherry tagging donors.

Catalog number RDB13924
Resource name pMK281 (mCherry2-Hygro)
Alternative name mCherry2-Hygro, 975
Clone info. A plasmid for construction of C-terminal mCherry tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Gene/insert name Discosoma sp. RFP cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Discosoma sp. RFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose)[Link]
            Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
            The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            専用MTA (Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用)[Link]
            備考 クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。
            本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。専用MTA(Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用)をご参照ください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB13924 pMK281 (mCherry2-Hygro) DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Auxin Inducible Degron (AID) System (English text)
            Featured content Auxin Inducible Degron (AID) System (Japanese text)
            Featured content Genome Editing (English text)
            Featured content Genome Editing (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13924_A6B8p1_v2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13_-40
            Sequence file: RDB13924_A6B8a_2.seq check
            >D03070A08_A6B8_1_M13(-40)_E02_14.ab1
                1 CGCCCGTTAC GCGCGTATAC GACTCACTAT AGGGCGAATT GGAGCTCGGA TCCGGTGCAG
               61 GCGCCGCTAG CATGGTGAGC AAGGGCGAGG AGGATAACAT GGCCATCATC AAGGAGTTCA
              121 TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG ATCGAGGGCG
              181 AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG ACCAAGGGTG
              241 GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CATGTACGGC TCCAAGGCCT
              301 ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC GAGGGCTTCA
              361 ATTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC CAGGACTCCT
              421 CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC TTCCCCTCCG
              481 ACGGCCCCGT AATGCAGTGT CGTACCATGG GCTGGGAGGC CTCCACTGAG CGGATGTACC
              541 CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGCAGAGGCT GAAGCTGAAG GACGGCGGCC
              601 ACTACGACGC TGAGGTCAAG ACCACCTACA AGGCCAAGAA GCCCGTGCAG CTGCCCGGCG
              661 CCTACAACGT CGACATCAAG TTGGACATCC TTTCCCACAA CGAGGACTAC ACCATCGTGG
              721 AACAGTACGA ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAG CTGTACAAGT
              781 AATCTAGATA ACTGATCATA ATCAGCCATA CCACATTTGT AGAGGTTTTA CTTGCTTTAA
              841 AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT GAATGCAATT GTTGTTGTTA
              901 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA
              961 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT
             1021 AACGCGTCGA TCATATTCAT AACCCTTAAT ATAACTTCGT ATAATGTATG CTATACGAAG
             1081 TTTATTAAGG TCTTGAAGAG GAGTTTAACG TCAGCAAAGC TAGATCTCGA CCTCGAATTT
            1141 CTACCCGGAT AAGGGGAAGG GCGGCATTTT TCCACAG
            //
            Primer: PGKpro_F
            Sequence file: RDB13924_A6B8b_2.seq check
            >D03070A08_A6B8_1_PGK-proF_C07_07.ab1
                1 AGTCGGCGCT CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCATCCATCT AGATCTCGAG
               61 CAGCTGAAGC TTACCGCTAG CATGGATAGA TCCGGAAAGC CTGAACTCAC CGCGACGTCT
              121 GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA GCTCTCGGAG
              181 GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA
              241 AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC
              301 GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT GACCTATTGC
              361 ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT
              421 GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG
              481 AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC
              541 ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC
              601 AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA
              661 GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA CAATGGCCGC
              721 ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA CGAGGTCGCC
              781 AACATCTTCT TCTGGAGGCC GTGGTTGGCT TGTATGGAGC AGCAGACGCG CTACTTCGAG
              841 CGGAGGCATC CGGAGCTTGC AGGATCGCCG CGGCTCCGGG CGTATATGCT CCGCATTGGT
              901 CTTGACCAAC TCTATCAGAG CTTGGTTGAC GGCAATTTCG ATGATGCAGC TTGGCGCAGG
              961 GTCGATGCGA CGCAATCGTC CGATCCGGGA GCCCGGGACT GTCGGGCCGT ACACAAATCG
             1021 CCCGCAGAAG CGCGGCCCGT CTGGGACCGA TGGGCTGTGT AGAAGTACTC GCCCGATAGT
             1081 GGAACCGACG GCCTCCAGCA CTTCGGTTCG GAAGGCAAGA TAGCTAGCCG CCCGGTCCCC
            1141 ACGAACCCGC GCAAGA
            //
            Primer: PGK_N_rev
            Sequence file: RDB13924_A6B8c_2.seq check
            >D03070A08_A6B8_1_PGK-N-rev_C06_09.ab1
                1 CATGGGACGC CTCCCCTACC CGGTAGATTT CGAGGTCGAG ATCCTAAGCT TGGCTGGACG
               61 TAAACTCCTC TTCAGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG
              121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA
              181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA
              241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG
              301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
              361 CTGATTATGA TCAGTTATCT AGATTACTTG TACAGCTCGT CCATGCCGCC GGTGGAGTGG
              421 CGGCCCTCGG CGCGTTCGTA CTGTTCCACG ATGGTGTAGT CCTCGTTGTG GGAAAGGATG
              481 TCCAACTTGA TGTCGACGTT GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT GGCCTTGTAG
              541 GTGGTCTTGA CCTCAGCGTC GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT CTGCTTGATC
              601 TCGCCCTTCA GGGCGCCGTC CTCGGGGTAC ATCCGCTCAG TGGAGGCCTC CCAGCCCATG
              661 GTACGACACT GCATTACGGG GCCGTCGGAG GGGAAGTTGG TGCCGCGCAG CTTCACCTTG
              721 TAGATGAACT CGCCGTCCTG CAGGGAGGAG TCCCTGGGTC ACGGTCACCA CGCCGCCGTC
              781 CTCGAAGTTC ATCACGCGCT CCCAATTGCA GCCCCTCGGG GGAAGGACAG CTTCAAGTAG
              841 TCCGGGGATG TCAGGCGGGG TGCTTTCACG TAGGGCCTTG GAGCCGTACA TGAACTGAGG
              901 GGACAGGATG TCCCAGGCCG AAAGGGAAGG GGGCCACCCC TTGGGTTCAC CTTTCAGCTT
              961 TGGCGGGTCT GGGTTGCCCC TCGTAAGGGG CGGGCCCTTC GCCCCTCGGC CCCTCCGATC
            1021 TATCGAATCT CGGTGGGCCG GTT
            //
            Primer: M13R
            Sequence file: RDB13924_A6B8d_2.seq check
            >D03070A08_A6B8_1_M13R_E01_13.ab1
                1 CATCGGGCAT TACCCTCACT AAAGGGACAA AAGCTGGGTA CCGGATCCTA GTGAACCTCT
               61 TCGAGGGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATATTA AGGGTTCCGG
              121 ATCTCGACCA GCTTCTGATG GAATTAGAAC TTGGCAAAAC AATACTGAGA ATGAAGTGTA
              181 TGTGGAACAG AGGCTGCTGA TCTCGTTCTT CAGGCTATGA AACTGACACA TTTGGAAACC
              241 ACAGTACTTA GAACCACAAA GTGGGAATCA AGAGAAAAAC AATGATCCCA CGAGAGATCT
              301 ATAGATCTAT AGATCATGAG TGGGAGGAAT GAGCTGGCCC TTAATTTGGT TTTGCTTGTT
              361 TAAATTATGA TATCCAACTA TGAAACATTA TCATAAAGCA ATAGTAAAGA GCCTTCAGTA
              421 AAGAGCAGGC ATTTATCTAA TCCCACCCCA CCCCCACCCC CGTAGCTCCA ATCCTTCCAT
              481 TCAAAATGTA GGTACTCTGT TCTCACCCTT CTTAACAAAG TATGACAGGA AAAACTTCCA
              541 TTTTAGTGGA CATCTTTATT GTTTAATAGA TCATCAATTT CTGCATCCCG GGGATCTGAT
              601 ATCATCGATG CATGGGGTCG TGCGCTCCTT TCGGTCGGGC GCTGCGGGTC GTGGGGCGGG
              661 CGGCTAGCCT ATTCCTTTGC CCTCGGACGA GTGCTGGGGC GTCGGTTTCC ACTATCGGCG
              721 AGTACTTCTA CACAGCCATC GGTCCAGACG GCCGCGCTTC TGCGGGCGAT TTGTGTACGC
              781 CCGACAGTCC CGGCTCCGGA TCGGACGATT GCGTCGCATC GACCCTGCGC CCAAGCTGCA
              841 ATCATCGAAA TTGCCGTCAA CCAAAGCTCT GATAGAGTTG GTCAAGACCA ATGCGGGAGC
              901 ATATACGCCC GGGAGCCGCG GCGATCCTGC AAGCTCCGGA TGCCCTCCGC TCGAAAGTAG
              961 CGCGTCTGCT GCTCCATAAC AAGCCCAACC AACGGGCCCT CCAGGAAGAA AGATGTTGGC
             1021 GACCTCGTAT TGGGGAATCC CCGAACAATC GCCCTCGCTT CCAGTCAAAT GACCGGCCTG
             1081 TTAATGCGGC CATTGTCGTC AAGGACCATT GATTGAAGCG AATCGGCGTG GCACGAGTGG
            1141 CCTGACCTTC GGGGGCACAG TTCCCTTT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.
            reference Natsume, T., Acute inactivation of the replicative helicase in human cells triggers MCM8-9-dependent DNA synthesis. Genes Dev. (2017). PMID 28487407.

            2022.02.09

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