RDB13924 - pMK281 (mCherry2-Hygro)

Resource data sheet
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pMK281 (mCherry2-Hygro)

A plasmid for construction of C-terminal mCherry tagging donors.

Catalog number	RDB13924
Resource name	pMK281 (mCherry2-Hygro)
Alternative name	mCherry2-Hygro, 975
Clone info.	A plasmid for construction of C-terminal mCherry tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
Vector backbone	pBluescriptII KS- (Plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Am^r
Gene/insert name	Discosoma sp. RFP cDNA
Depositor	Kanemaki, Masato \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

Ordering Please visit Information of Request for Distribution.
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Exclusive MTA [Link]

Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
Please use the Exclusive MTA (Clontech FPs).

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Exclusive MTA [Link]
Remarks	An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required. Please use the Exclusive MTA (Clontech FPs).

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 専用MTA（英語版のみ）[Link]
備考	クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。専用MTA（Clontech FPs, 英語版のみ）をお使いください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13924	pMK281 (mCherry2-Hygro)	DNA solution

cDNA Request [in Japanese] [in English]

References and tips

Electronic file

Featured content	Auxin Inducible Degron (AID) System (English text)
Featured content	Auxin Inducible Degron (AID) System (Japanese text)
Featured content	Genome Editing (English text)
Featured content	Genome Editing (Japanese text)
Featured content	Empty Backbone (English text)
Featured content	Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB13924_A6B8p1_v2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40	RDB13924_A6B8a_2.seq
>D03070A08_A6B8_1_M13(-40)_E02_14.ab1 1 CGCCCGTTAC GCGCGTATAC GACTCACTAT AGGGCGAATT GGAGCTCGGA TCCGGTGCAG 61 GCGCCGCTAG CATGGTGAGC AAGGGCGAGG AGGATAACAT GGCCATCATC AAGGAGTTCA 121 TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG ATCGAGGGCG 181 AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG ACCAAGGGTG 241 GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CATGTACGGC TCCAAGGCCT 301 ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC GAGGGCTTCA 361 ATTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC CAGGACTCCT 421 CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC TTCCCCTCCG 481 ACGGCCCCGT AATGCAGTGT CGTACCATGG GCTGGGAGGC CTCCACTGAG CGGATGTACC 541 CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGCAGAGGCT GAAGCTGAAG GACGGCGGCC 601 ACTACGACGC TGAGGTCAAG ACCACCTACA AGGCCAAGAA GCCCGTGCAG CTGCCCGGCG 661 CCTACAACGT CGACATCAAG TTGGACATCC TTTCCCACAA CGAGGACTAC ACCATCGTGG 721 AACAGTACGA ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAG CTGTACAAGT 781 AATCTAGATA ACTGATCATA ATCAGCCATA CCACATTTGT AGAGGTTTTA CTTGCTTTAA 841 AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT GAATGCAATT GTTGTTGTTA 901 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA 961 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT 1021 AACGCGTCGA TCATATTCAT AACCCTTAAT ATAACTTCGT ATAATGTATG CTATACGAAG 1081 TTTATTAAGG TCTTGAAGAG GAGTTTAACG TCAGCAAAGC TAGATCTCGA CCTCGAATTT 1141 CTACCCGGAT AAGGGGAAGG GCGGCATTTT TCCACAG //
Primer: PGKpro_F	RDB13924_A6B8b_2.seq
>D03070A08_A6B8_1_PGK-proF_C07_07.ab1 1 AGTCGGCGCT CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCATCCATCT AGATCTCGAG 61 CAGCTGAAGC TTACCGCTAG CATGGATAGA TCCGGAAAGC CTGAACTCAC CGCGACGTCT 121 GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA GCTCTCGGAG 181 GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA 241 AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC 301 GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT GACCTATTGC 361 ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT 421 GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG 481 AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC 541 ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC 601 AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA 661 GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA CAATGGCCGC 721 ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA CGAGGTCGCC 781 AACATCTTCT TCTGGAGGCC GTGGTTGGCT TGTATGGAGC AGCAGACGCG CTACTTCGAG 841 CGGAGGCATC CGGAGCTTGC AGGATCGCCG CGGCTCCGGG CGTATATGCT CCGCATTGGT 901 CTTGACCAAC TCTATCAGAG CTTGGTTGAC GGCAATTTCG ATGATGCAGC TTGGCGCAGG 961 GTCGATGCGA CGCAATCGTC CGATCCGGGA GCCCGGGACT GTCGGGCCGT ACACAAATCG 1021 CCCGCAGAAG CGCGGCCCGT CTGGGACCGA TGGGCTGTGT AGAAGTACTC GCCCGATAGT 1081 GGAACCGACG GCCTCCAGCA CTTCGGTTCG GAAGGCAAGA TAGCTAGCCG CCCGGTCCCC 1141 ACGAACCCGC GCAAGA //
Primer: PGK_N_rev	RDB13924_A6B8c_2.seq
>D03070A08_A6B8_1_PGK-N-rev_C06_09.ab1 1 CATGGGACGC CTCCCCTACC CGGTAGATTT CGAGGTCGAG ATCCTAAGCT TGGCTGGACG 61 TAAACTCCTC TTCAGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG 121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA 181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA 241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG 301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG 361 CTGATTATGA TCAGTTATCT AGATTACTTG TACAGCTCGT CCATGCCGCC GGTGGAGTGG 421 CGGCCCTCGG CGCGTTCGTA CTGTTCCACG ATGGTGTAGT CCTCGTTGTG GGAAAGGATG 481 TCCAACTTGA TGTCGACGTT GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT GGCCTTGTAG 541 GTGGTCTTGA CCTCAGCGTC GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT CTGCTTGATC 601 TCGCCCTTCA GGGCGCCGTC CTCGGGGTAC ATCCGCTCAG TGGAGGCCTC CCAGCCCATG 661 GTACGACACT GCATTACGGG GCCGTCGGAG GGGAAGTTGG TGCCGCGCAG CTTCACCTTG 721 TAGATGAACT CGCCGTCCTG CAGGGAGGAG TCCCTGGGTC ACGGTCACCA CGCCGCCGTC 781 CTCGAAGTTC ATCACGCGCT CCCAATTGCA GCCCCTCGGG GGAAGGACAG CTTCAAGTAG 841 TCCGGGGATG TCAGGCGGGG TGCTTTCACG TAGGGCCTTG GAGCCGTACA TGAACTGAGG 901 GGACAGGATG TCCCAGGCCG AAAGGGAAGG GGGCCACCCC TTGGGTTCAC CTTTCAGCTT 961 TGGCGGGTCT GGGTTGCCCC TCGTAAGGGG CGGGCCCTTC GCCCCTCGGC CCCTCCGATC 1021 TATCGAATCT CGGTGGGCCG GTT //
Primer: M13R	RDB13924_A6B8d_2.seq
>D03070A08_A6B8_1_M13R_E01_13.ab1 1 CATCGGGCAT TACCCTCACT AAAGGGACAA AAGCTGGGTA CCGGATCCTA GTGAACCTCT 61 TCGAGGGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATATTA AGGGTTCCGG 121 ATCTCGACCA GCTTCTGATG GAATTAGAAC TTGGCAAAAC AATACTGAGA ATGAAGTGTA 181 TGTGGAACAG AGGCTGCTGA TCTCGTTCTT CAGGCTATGA AACTGACACA TTTGGAAACC 241 ACAGTACTTA GAACCACAAA GTGGGAATCA AGAGAAAAAC AATGATCCCA CGAGAGATCT 301 ATAGATCTAT AGATCATGAG TGGGAGGAAT GAGCTGGCCC TTAATTTGGT TTTGCTTGTT 361 TAAATTATGA TATCCAACTA TGAAACATTA TCATAAAGCA ATAGTAAAGA GCCTTCAGTA 421 AAGAGCAGGC ATTTATCTAA TCCCACCCCA CCCCCACCCC CGTAGCTCCA ATCCTTCCAT 481 TCAAAATGTA GGTACTCTGT TCTCACCCTT CTTAACAAAG TATGACAGGA AAAACTTCCA 541 TTTTAGTGGA CATCTTTATT GTTTAATAGA TCATCAATTT CTGCATCCCG GGGATCTGAT 601 ATCATCGATG CATGGGGTCG TGCGCTCCTT TCGGTCGGGC GCTGCGGGTC GTGGGGCGGG 661 CGGCTAGCCT ATTCCTTTGC CCTCGGACGA GTGCTGGGGC GTCGGTTTCC ACTATCGGCG 721 AGTACTTCTA CACAGCCATC GGTCCAGACG GCCGCGCTTC TGCGGGCGAT TTGTGTACGC 781 CCGACAGTCC CGGCTCCGGA TCGGACGATT GCGTCGCATC GACCCTGCGC CCAAGCTGCA 841 ATCATCGAAA TTGCCGTCAA CCAAAGCTCT GATAGAGTTG GTCAAGACCA ATGCGGGAGC 901 ATATACGCCC GGGAGCCGCG GCGATCCTGC AAGCTCCGGA TGCCCTCCGC TCGAAAGTAG 961 CGCGTCTGCT GCTCCATAAC AAGCCCAACC AACGGGCCCT CCAGGAAGAA AGATGTTGGC 1021 GACCTCGTAT TGGGGAATCC CCGAACAATC GCCCTCGCTT CCAGTCAAAT GACCGGCCTG 1081 TTAATGCGGC CATTGTCGTC AAGGACCATT GATTGAAGCG AATCGGCGTG GCACGAGTGG 1141 CCTGACCTTC GGGGGCACAG TTCCCTTT //

Please visit Sequencing and PCR primers for primer information.

References

original	Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.
reference	Natsume, T., Acute inactivation of the replicative helicase in human cells triggers MCM8-9-dependent DNA synthesis. Genes Dev. (2017). PMID 28487407.

2018.09.03

GNP_filter3_RDBDEP_html_180823.pl