Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK292 (mAID-mCherry2-NeoR)

A plasmid for construction of C-terminal mAID-mCherry tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	提供依頼書 [Word] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13935_A6B8p1-4.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410) Region: insert 5'(Linker,mAID,mCherry2 5') Sequence file: RDB13935_A6B8i.seq
>D03070A19_A6B8_1_M13(-40)_D05_11.ab1     1 NNNNNNNNNN NNNNNNNNCN NNNNNNGANT NNNNNTAGGG CGAANTGGAG CTCCCCGGGN    61 TCCGGTGCAG GCGCCAAGGA GAAGAGTGCT TGTCCTAAAG ATCCAGCCAA ACCTCCGGCC   121 AAGGCACAAG TTGTGGGATG GCCACCGGTG AGATCATACC GGAAGAACGT GATGGTTTCC   181 TGCCAAAAAT CAAGCGGTGG CCCGGAGGCG GCGGCGTTCG TGAAGGTATC AATGGACGGA   241 GCACCGTACT TGAGGAAAAT CGATTTGAGG ATGTATAAAG CTAGCATGGT GAGCAAGGGC   301 GAGGAGGATA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC   361 TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC   421 ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC   481 CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC   541 GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAATTGGG AGCGCGTGAT GAACTTCGAG   601 GACGGCGGCG TGGTGACCGT GACNNNNNAC TCCTCCCTGC AGGACGGCGA GTTCATCTAC   661 AAGGTGAAGC TGCGCGGCAC CNACTTCCCC TCCGACGGCC CCGTAATGCA GTGTCGTACC   721 ATGGGCTGGG AGGCCTCCAC TGNNNNGATG TNNNCCCGAG GACGGCGCCC TGAANGGCGA   781 GATCNAGCAG ANGCTGAANG CTGANNGACG GCGGCCACTA CNACGCTGAN GTCAAGACCA   841 CCTANNNNGC CNAGAAGCCC GTGCAGCTGN CCNGCGCNNN NNACGTCNAN NNCNNGTTNG   901 GANATCCTTN NNNNNNNCNN GGACTANNNC NTCGNGGAAN NANNNNNNAA NNNNNNNNNG   961 GGNCGNCNNC TNNNNCNGNN GGCNNNGNNN NNNCNGTNCA NNNNNTCTNN NANNANCTGA  1021 NNNNNNANNC NNNNNTNNCA CATTTNNNNN NANGGNTTTT ANNNNNNNTT TNNNNNNNNN 1081 N //
Primer: M13R (Pr0160) Region: insert 3'(NeoR_KanR 3') Sequence file: RDB13935_A6B8j.seq
>D03070A19_A6B8_1_M13R_D04_10.ab1     1 NNNNNNNNNN NNNNNNNNNN ANGGGNNNNN AAGCTGGGTA NCGGATCCNA CGACCCAACA    61 CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC   121 TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC   181 CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA   241 TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC   301 ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC   361 GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC   421 CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGG TCCGCCACAC   481 CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA   541 AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC   601 TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA   661 CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA   721 ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA   781 CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA   841 GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCNAGCAC AGCTGCNCAN NGAACGCCCG   901 NCGNGGCCAG CCACGATAGC CGCGCNGCCN CGTCTNGCAG TTCANTCNNN GCANCNGNNN 961 GGTCNGTCTN GANNAAAANA NNCNGGCGCC CNNNCNNNNN NNNNCNNNNN NNNNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert mid(SV40 ter,mCherry2 3') Sequence file: RDB13935_A6B8k.seq
>13935_13935_A9KsF_SV40polyA_R2_F09_18_ABI24.ab1     1 NNNNNNNNGN ANNANNNNGT TAACAACAAC AATTGCATTC ATTTTATGTT TCNGGTTCAG    61 GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGCTGAT   121 TATGATCAGT TATCTAGATT ACTTGTACAG CTCGTCCATG CCGCCGGTGG AGTGGCGGCC   181 CTCGGCGCGT TCGTACTGTT CCACGATGGT GTAGTCCTCG TTGTGGGAAA GGATGTCCAA   241 CTTGATGTCG ACGTTGTAGG CGCCGGGCAG CTGCACGGGC TTCTTGGCCT TGTAGGTGGT   301 CTTGACCTCA GCGTCGTAGT GGCCGCCGTC CTTCAGCTTC AGCCTCTGCT TGATCTCGCC   361 CTTCAGGGCG CCGTCCTCGG GGTACATCCG CTCAGTGGAG GCCTCCCAGC CCATGGTACG   421 ACACTGCATT ACGGGGCCGT CGGAGGGGAA GTTGGTGCCG CGCAGCTTCA CCTTGTAGAT   481 GAACTCGCCG TCCTGCAGGG AGGAGTCCTG GGTCACGGTC ACCACGCCGC CGTCCTCGAA   541 GTTCATCACG CGCTCCCAAT TGAAGCCCTC GGGGAAGGAC AGCTTCAAGT AGTCGGGGAT   601 GTCGGCGGGG TGCTTCACGT AGGCCTTGGA GCCGTACATG AACTGAGGGG ACAGGATGTC   661 CCAGGCGAAG GGCAGGGGGC CACCCTTGGT CACCTTCAGC TTGNCGGTCT GGGTGCCCTC   721 GTANGGGCGG NCCTCGCCCT CGCCCTCGAT CTCGAACTCG TGGCCGTTCA CGGAGCCCTC   781 CATGTGCANC TTGAAGCGCA TGAACTCCTT GATGATGGCC ATGTTATCCT CCTCGCCCTT   841 GCTCACCATG CTAGCTTTAT ACATCCTCAA NTCGATTTTC CTCNAGTACN GNGCNNCGNN   901 NATTGATNNC TTCACNAACG CCGCCGCNNN CNGGNCACCG CTNGANTTTT GGNNNNAANN 961 NNTCNCGTTC NNNCGGTATG ATNNNNNNNN NN //
Primer: SV40pro_F_V2 (Pr0154) Region: insert mid(NeoR_KanR 5 ') Sequence file: RDB13935_A6B8l.seq
>D03070A19_A6B8_1_SV40pro_F_V2_F10_16.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGCTTTTG CAAGNNCGAT CNAGAGANNG GATGAGGNTC    61 GTTTCGCATG ATTGAACNNG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG   121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG   181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA   241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC   301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC   361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA   421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA   481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT   541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT   601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT   661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA   721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGANNAG CTTGGCGGCG AATGGGCTGA   781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TNCCGATTCG CAGCGCATCG CCTTCTATCG   841 CCTTCTTGAC GANTTCTTCT GAGCGGGNNT CTGGGGTTCG AANTGACCGA NCAAGCGNNG   901 CCCNANCTGN CNTCNCGANN NTNCNATTCC NCCGCCGCCT TCTATGAANN NNNGGGGCTT 961 NNNNNNNNNT TTNCNNGNNN NNNNGNTNNN NGNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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