Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK292 (mAID-mCherry2-NeoR)

A plasmid for construction of C-terminal mAID-mCherry tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Please contact us to request the Exclusive MTA.
Remarks	This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。
備考	Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13935_A6B8p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410) Region: insert5'(Linker,mAID,mCherry2) Sequence file: RDB13935_A6B8e.seq
>D03070A19_A6B8_1_M13(-40)_D05_11.ab1     1 CCGGGCAGAT GGAGCGCGCG TAATACGACT CACTATAGGG CGAATTGGAG CTCCCCGGGA    61 TCCGGTGCAG GCGCCAAGGA GAAGAGTGCT TGTCCTAAAG ATCCAGCCAA ACCTCCGGCC   121 AAGGCACAAG TTGTGGGATG GCCACCGGTG AGATCATACC GGAAGAACGT GATGGTTTCC   181 TGCCAAAAAT CAAGCGGTGG CCCGGAGGCG GCGGCGTTCG TGAAGGTATC AATGGACGGA   241 GCACCGTACT TGAGGAAAAT CGATTTGAGG ATGTATAAAG CTAGCATGGT GAGCAAGGGC   301 GAGGAGGATA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC   361 TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC   421 ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC   481 CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC   541 GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAATTGGG AGCGCGTGAT GAACTTCGAG   601 GACGGCGGCG TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA GTTCATCTAC   661 AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GTGTCGTACC   721 ATGGGCTGGG AGGCCTCCAC TGAGCGGATG TAACCCCGAG GACGGCGCCC TGAAGGGCGA   781 GATCAAGCAG AGGCTGAAAG CTGAAGGACG GCGGCCACTA CGACGCTGAG GTCAAGACCA   841 CCTACAAGGC CAAGAAGCCC GTGCAGCTGC CCGGCGCCTA CAACGTCGAC ATCAAGTTGG   901 GACATCCTTT CCCACAACGA GGACTACACC ATCGTGGAAA CAGTACGAAA CGCGCCGAGG   961 GGCCGCCCAC TCCACCGGCC GGCATGGAAC GAGCTGTACA AGTAATCTAA GATAAACTGA  1021 TACATAAATC AGCCATACCA CATTTGGTAA GAGGGTTTTT ACTATGCTTT TAAAAAACTT  1081 CCCACACCCT TCCCCCTTGA ACTGAAACCA TAGAATGAAT GCAATTGATT GTGGGTAACT  1141 TGGTTTTATT GGCAAGGCCT TATCAACGTG GGTTTGTAGC AGACAATTGA AGAGCCGTCA 1201 T //
Primer: SV40polyA-R (Pr0109) Region: insert mid(SV40 ter,mCherry2) Sequence file: RDB13935_A6B8f.seq
>D03070A19_A6B8_1_SV40polyA-R_H05_23.ab1     1 CGCCTCGCAG TTATTAGCAG TAACCTCTAC AATGTGGTAT GGCTGATTAT GATCAGTTAT    61 CTAGATTACT TGTACAGCTC GTCCATGCCG CCGGTGGAGT GGCGGCCCTC GGCGCGTTCG   121 TACTGTTCCA CGATGGTGTA GTCCTCGTTG TGGGAAAGGA TGTCCAACTT GATGTCGACG   181 TTGTAGGCGC CGGGCAGCTG CACGGGCTTC TTGGCCTTGT AGGTGGTCTT GACCTCAGCG   241 TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC CTCTGCTTGA TCTCGCCCTT CAGGGCGCCG   301 TCCTCGGGGT ACATCCGCTC AGTGGAGGCC TCCCAGCCCA TGGTACGACA CTGCATTACG   361 GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC AGCTTCACCT TGTAGATGAA CTCGCCGTCC   421 TGCAGGGAGG AGTCCTGGGT CACGGTCACC ACGCCGCCGT CCTCGAAGTT CATCACGCGC   481 TCCCAATTGA AGCCCTCGGG GAAGGACAGC TTCAAGTAGT CGGGGATGTC GGCGGGGTGC   541 TTCACGTAGG CCTTGGAGCC GTACATGAAC TGAGGGGACA GGATGTCCCA GGCGAAGGGC   601 AGGGGGCCAC CCTTGGTCAC CTTCAGCTTG GCGGTCTGGG TGCCCTCGTA GGGGCGGCCC   661 TCGCCCTCGC CCTCGATCTC GAACTCGTGG CCGTTCACGG AGCCCTCCAT GTGCACCTTG   721 AAGCGCATGA ACTCCTTGAT GATGGCCATG TTATCCTCCT CGCCCTTGCT CACCATGCTA   781 GCTTTATACA TCCTCAAATC GATTTTCCTC AAGTACGGTG CTCCGTCCAT TGATACCTTC   841 ACGAACGCCG CCCGCCTCCG GGCCACCGCT TGATTTTTGG CAGGAAACCA TCACGTTCTT   901 CCGGTATGAT CTCACCGGTG GCCATCCCAC AACTTGTGCC CTTGGCCGGA AGGTTTGGGC   961 TGGGATCTTT AGGAACAAGC AACTCCTTCT CCCTTGGCGC CTGCACCGGA ATCCCGGGGG  1021 GAGCTCCAAT TCGCCCTATA AGTGAGTCGT AATACGCGCG CTCCCACTGG CGGTCGATTT  1081 ACCACGTCCT GACTGGAAAC CCTGCGGTTA CCCACTTAAA TCGCCCTTGG CAGCCACAAT 1141 CCCCCATATC AGCCACAAGC T //
Primer: SV40pro_F_V2 (Pr0154) Region: insert mid(NeoR) Sequence file: RDB13935_A6B8g.seq
>D03070A19_A6B8_1_SV40pro_F_V2_F10_16.ab1     1 GTTGGCCCTT TTTTGGAGGC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC    61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG   121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG   181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA   241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC   301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC   361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA   421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA   481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT   541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT   601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT   661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA   721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA   781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG   841 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG   901 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGGCTT   961 CGGAATCGTT TTCCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG  1021 GAGTTCTTTC GCCCACCCTA GGGGGAAGGC TAACTGAAAT CACGAAGGAG ACAATACCGG 1081 AAGGACCGCG CTATGAACCG GCAATTAAAA AGACAGGAAT AAAAACGGCA //
Primer: M13R (Pr0160) Region: insert3'(NeoR) Sequence file: RDB13935_A6B8h.seq
>D03070A19_A6B8_1_M13R_D04_10.ab1     1 GCCGCGGCAT TACCTCACTA AAGGGAACAA AAGCTGGGTA CCGGATCCAA CGACCCAACA    61 CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC   121 TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC   181 CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA   241 TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC   301 ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC   361 GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC   421 CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGG TCCGCCACAC   481 CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA   541 AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC   601 TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA   661 CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA   721 ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA   781 CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA   841 GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG   901 TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA   961 GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCCGGAA CACGCGGCAT  1021 CAGAGCAGCC CGATTGTCTG TGTGCCCAGT CATAGGCCGG ATAGGCCTCT CCACCCCAAG  1081 GCGGGCCAGG AGATCTTGCG ATGCCAGTTC CAATTCTTGA TCAGTCATGC CGAAAACCGA 1141 TTCCCT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.02.27

GNP_filter3_RDBDEP_html_230227.pl