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pMK280 (mCherry2-NeoR)

A plasmid for construction of C-terminal mCherry tagging donors.

Catalog number RDB13923
Resource name pMK280 (mCherry2-NeoR)
Alternative name mCherry2-NeoR, 970
Clone info. A plasmid for construction of C-terminal mCherry tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone. Please note that this plasmid does not contain the loxP sites, which are intended for removal of the marker.
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Gene/insert name Discosoma sp. RFP cDNA
Depositor Kanemaki, Masato |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
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RDB13923 pMK280 (mCherry2-NeoR) DNA solution

cDNA Request [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)
Featured content Genome Editing (English text)
Featured content Genome Editing (Japanese text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

References

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13923_A6B8p1_v2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB13923_A6B8a_2.seq
>D03070A07_A6B8_1_M13(-40)_D02_11.ab1
    1 CGCGGACGCG CGTATACGAC TCACTATAGG GCGAATTGGA GCTCGGATCC GGTGCAGGCG
   61 CCGCTAGCAT GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC
  121 GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG
  181 GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC
  241 CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG
  301 TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAATT
  361 GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC
  421 TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG
  481 GCCCCGTAAT GCAGTGTCGT ACCATGGGCT GGGAGGCCTC CACTGAGCGG ATGTACCCCG
  541 AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT
  601 ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT
  661 ACAACGTCGA CATCAAGTTG GACATCCTTT CCCACAACGA GGACTACACC ATAGATGGAA
  721 CAGTACGAAC GCGCCGAGGG CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGTAA
  781 TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA
  841 AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC
  901 TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT
  961 AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAT GTATCTACGC
 1021 GTAAAATTGG TAAGGCGTTA TATTTTGTTA AATTCGCGTT AAATTTTTGT AATCAGCTCA
 1081 TTTTTTACAT AGCGAAATCG CAAATCCTAT ATCAAAGAAT AGACGAGAAT AGGCTTTGAA
1141 GTGTTGTTTC CAAGAATCG
//
Primer: SV40pro_F_V2 RDB13923_A6B8b_2.seq
>D03070A07_A6B8_1_SV40pro_F_V2_B10_04.ab1
    1 GTAGCACATT TTTGGAGCTA GGCTTTTGCA AGATCGATCA AGAGACAGGA TGAGGATCGT
   61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC
  601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  781 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  841 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGA AATGACCGAC CAAGCGACGC
  901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG
  961 GAATTCGTTT TCCGGGACGC CGGCTGGATG ATCCCTCCAG CGCGGGGATC TCATGCTGGG
 1021 AGTTTCTTCG CCCACCCTAG GGGAGGCTAA CCTGAACACG GGAAAGGAGA CAATACCGGA
1081 AAGAACCGCG CTAATGAACG GCAAATAAAA AAGACAGAAA ATAAAAAACC
//
Primer: SV40polyA-R RDB13923_A6B8c_2.seq
>D03070A07_A6B8_1_SV40polyA-R_G04_19.ab1
    1 CCCCATGCAG TTATTAGCAG TAACCTCTAC AATGTGGTAT GGCTGATTAT GATCAGTTAT
   61 CTAGATTACT TGTACAGCTC GTCCATGCCG CCGGTGGAGT GGCGGCCCTC GGCGCGTTCG
  121 TACTGTTCCA CGATGGTGTA GTCCTCGTTG TGGGAAAGGA TGTCCAACTT GATGTCGACG
  181 TTGTAGGCGC CGGGCAGCTG CACGGGCTTC TTGGCCTTGT AGGTGGTCTT GACCTCAGCG
  241 TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC CTCTGCTTGA TCTCGCCCTT CAGGGCGCCG
  301 TCCTCGGGGT ACATCCGCTC AGTGGAGGCC TCCCAGCCCA TGGTACGACA CTGCATTACG
  361 GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC AGCTTCACCT TGTAGATGAA CTCGCCGTCC
  421 TGCAGGGAGG AGTCCTGGGT CACGGTCACC ACGCCGCCGT CCTCGAAGTT CATCACGCGC
  481 TCCCAATTGA AGCCCTCGGG GAAGGACAGC TTCAAGTAGT CGGGGATGTC GGCGGGGTGC
  541 TTCACGTAGG CCTTGGAGCC GTACATGAAC TGAGGGGACA GGATGTCCCA GGCGAAGGGC
  601 AGGGGGCCAC CCTTGGTCAC CTTCAGCTTG GCGGTCTGGG TGCCCTCGTA GGGGCGGCCC
  661 TCGCCCTCGC CCTCGATCTC GAACTCGTGG CCGTTCACGG AGCCCTCCAT GTGCACCTTG
  721 AAGCGCATGA ACTCCTTGAA TGATGGCCAT GTTATCCTCC TCGCCCTTGC TCACCATGCT
  781 AGCGGCGCCT GCACCGGATC CGAGCTCCAA TTCGCCCTAT AAGTGAGTCG TATTACGCGC
  841 GCTCACTGGC GGTCGTTTTA CAACGTCGTG ACTGGGAAAA AACCCTGGCG TTACCCAAAC
  901 TTAAATCGCC TTGCAGCACA TCCCCCCTTT CGCCAGCTGG CGTAATAAGC GAAGAGGCCC
  961 GCACCGATCG CCCTTCCCAA CAAATTGCGC AGCCCTGAAT GGCCAAATGG AAAATTGTAA
1021 AGCGGTAAAT ATTTTGGTTA AAAAATCCCG CGAT
//
Primer: M13R RDB13923_A6B8d_2.seq
>D03070A07_A6B8_1_M13R_D01_10.ab1
    1 CATCGCGCAA TTACCCCTCA CTAAGGGAAC AAAAGCTGGG TACCGGGCCC CCCCTCGAGG
   61 GATCCAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG
  121 TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA
  181 ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC
  241 CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG
  301 GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG
  361 GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG
  421 GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG
  481 ATAGCGGTCC GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC
  541 CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG
  601 CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC
  661 CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG
  721 TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC
  781 ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC
  841 CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCAACAG
  901 CTGCGCAAGG AAACGCCCGT CGTGGCCCAG CCACGATAAG CCCGCGCTGC CTCGTCTTGC
  961 AGTTTCATTC AAGGGCACCG GACAGGGTCG GGTCTTGAAC AAAAAAGAAC CGGGCGCTCC
 1021 CTGCCGCTGA CAGCCCGAAC AACGGCGGGC AATCAGAGCA AGCCGAATTG GTCTTGTTTG
 1081 TTGCCCAGTT CAATAGCCCG GAATAGCCTC CTCAACCCAA AGCGGTCTGG AAGATCCTGC
1141 TTGCAAATCC ATTCTTTGTT TTCAATTCTC AATGTCCCAG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

2018.09.03

GNP_filter3_RDBDEP_html_180823.pl