Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK280 (mCherry2-NeoR)

A plasmid for construction of C-terminal mCherry tagging donors.

Catalog number RDB13923
Resource name pMK280 (mCherry2-NeoR)
Alternative name mCherry2-NeoR, 970
Clone info. A plasmid for construction of C-terminal mCherry tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone. Please note that this plasmid does not contain the loxP sites, which are intended for removal of the marker.
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Gene/insert name Discosoma sp. RFP cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Discosoma sp. RFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose)[Link]
            Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
            The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose).
            Distribution information (domestic users) [open/close]


            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB13923 pMK280 (mCherry2-NeoR) DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Auxin Inducible Degron (AID) System (English text)
            Featured content Auxin Inducible Degron (AID) System (Japanese text)
            Featured content Genome Editing (English text)
            Featured content Genome Editing (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13923_A6B8p1_v2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13_-40
            Sequence file: RDB13923_A6B8a_2.seq check
            >D03070A07_A6B8_1_M13(-40)_D02_11.ab1
                1 CGCGGACGCG CGTATACGAC TCACTATAGG GCGAATTGGA GCTCGGATCC GGTGCAGGCG
               61 CCGCTAGCAT GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC
              121 GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG
              181 GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC
              241 CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG
              301 TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAATT
              361 GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC
              421 TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG
              481 GCCCCGTAAT GCAGTGTCGT ACCATGGGCT GGGAGGCCTC CACTGAGCGG ATGTACCCCG
              541 AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT
              601 ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT
              661 ACAACGTCGA CATCAAGTTG GACATCCTTT CCCACAACGA GGACTACACC ATAGATGGAA
              721 CAGTACGAAC GCGCCGAGGG CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGTAA
              781 TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA
              841 AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC
              901 TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT
              961 AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAT GTATCTACGC
             1021 GTAAAATTGG TAAGGCGTTA TATTTTGTTA AATTCGCGTT AAATTTTTGT AATCAGCTCA
             1081 TTTTTTACAT AGCGAAATCG CAAATCCTAT ATCAAAGAAT AGACGAGAAT AGGCTTTGAA
            1141 GTGTTGTTTC CAAGAATCG
            //
            Primer: SV40pro_F_V2
            Sequence file: RDB13923_A6B8b_2.seq check
            >D03070A07_A6B8_1_SV40pro_F_V2_B10_04.ab1
                1 GTAGCACATT TTTGGAGCTA GGCTTTTGCA AGATCGATCA AGAGACAGGA TGAGGATCGT
               61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
              121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
              181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
              241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
              301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
              361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
              421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
              481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
              541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC
              601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
              661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
              721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
              781 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
              841 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGA AATGACCGAC CAAGCGACGC
              901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG
              961 GAATTCGTTT TCCGGGACGC CGGCTGGATG ATCCCTCCAG CGCGGGGATC TCATGCTGGG
             1021 AGTTTCTTCG CCCACCCTAG GGGAGGCTAA CCTGAACACG GGAAAGGAGA CAATACCGGA
            1081 AAGAACCGCG CTAATGAACG GCAAATAAAA AAGACAGAAA ATAAAAAACC
            //
            Primer: SV40polyA-R
            Sequence file: RDB13923_A6B8c_2.seq check
            >D03070A07_A6B8_1_SV40polyA-R_G04_19.ab1
                1 CCCCATGCAG TTATTAGCAG TAACCTCTAC AATGTGGTAT GGCTGATTAT GATCAGTTAT
               61 CTAGATTACT TGTACAGCTC GTCCATGCCG CCGGTGGAGT GGCGGCCCTC GGCGCGTTCG
              121 TACTGTTCCA CGATGGTGTA GTCCTCGTTG TGGGAAAGGA TGTCCAACTT GATGTCGACG
              181 TTGTAGGCGC CGGGCAGCTG CACGGGCTTC TTGGCCTTGT AGGTGGTCTT GACCTCAGCG
              241 TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC CTCTGCTTGA TCTCGCCCTT CAGGGCGCCG
              301 TCCTCGGGGT ACATCCGCTC AGTGGAGGCC TCCCAGCCCA TGGTACGACA CTGCATTACG
              361 GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC AGCTTCACCT TGTAGATGAA CTCGCCGTCC
              421 TGCAGGGAGG AGTCCTGGGT CACGGTCACC ACGCCGCCGT CCTCGAAGTT CATCACGCGC
              481 TCCCAATTGA AGCCCTCGGG GAAGGACAGC TTCAAGTAGT CGGGGATGTC GGCGGGGTGC
              541 TTCACGTAGG CCTTGGAGCC GTACATGAAC TGAGGGGACA GGATGTCCCA GGCGAAGGGC
              601 AGGGGGCCAC CCTTGGTCAC CTTCAGCTTG GCGGTCTGGG TGCCCTCGTA GGGGCGGCCC
              661 TCGCCCTCGC CCTCGATCTC GAACTCGTGG CCGTTCACGG AGCCCTCCAT GTGCACCTTG
              721 AAGCGCATGA ACTCCTTGAA TGATGGCCAT GTTATCCTCC TCGCCCTTGC TCACCATGCT
              781 AGCGGCGCCT GCACCGGATC CGAGCTCCAA TTCGCCCTAT AAGTGAGTCG TATTACGCGC
              841 GCTCACTGGC GGTCGTTTTA CAACGTCGTG ACTGGGAAAA AACCCTGGCG TTACCCAAAC
              901 TTAAATCGCC TTGCAGCACA TCCCCCCTTT CGCCAGCTGG CGTAATAAGC GAAGAGGCCC
              961 GCACCGATCG CCCTTCCCAA CAAATTGCGC AGCCCTGAAT GGCCAAATGG AAAATTGTAA
            1021 AGCGGTAAAT ATTTTGGTTA AAAAATCCCG CGAT
            //
            Primer: M13R
            Sequence file: RDB13923_A6B8d_2.seq check
            >D03070A07_A6B8_1_M13R_D01_10.ab1
                1 CATCGCGCAA TTACCCCTCA CTAAGGGAAC AAAAGCTGGG TACCGGGCCC CCCCTCGAGG
               61 GATCCAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG
              121 TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA
              181 ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC
              241 CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG
              301 GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG
              361 GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG
              421 GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG
              481 ATAGCGGTCC GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC
              541 CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG
              601 CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC
              661 CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG
              721 TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC
              781 ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC
              841 CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCAACAG
              901 CTGCGCAAGG AAACGCCCGT CGTGGCCCAG CCACGATAAG CCCGCGCTGC CTCGTCTTGC
              961 AGTTTCATTC AAGGGCACCG GACAGGGTCG GGTCTTGAAC AAAAAAGAAC CGGGCGCTCC
             1021 CTGCCGCTGA CAGCCCGAAC AACGGCGGGC AATCAGAGCA AGCCGAATTG GTCTTGTTTG
             1081 TTGCCCAGTT CAATAGCCCG GAATAGCCTC CTCAACCCAA AGCGGTCTGG AAGATCCTGC
            1141 TTGCAAATCC ATTCTTTGTT TTCAATTCTC AATGTCCCAG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

            2021.11.02

            GNP_filter3_RDBDEP_html_211029.pl