RDB13923 - pMK280 (mCherry2-NeoR)

Resource data sheet
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pMK280 (mCherry2-NeoR)

A plasmid for construction of C-terminal mCherry tagging donors.

Catalog number	RDB13923
Resource name	pMK280 (mCherry2-NeoR)
Alternative name	mCherry2-NeoR, 970
Clone info.	A plasmid for construction of C-terminal mCherry tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone. Please note that this plasmid does not contain the loxP sites, which are intended for removal of the marker.
Vector backbone	pBluescriptII KS- (Plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Am^r
Gene/insert name	Discosoma sp. RFP cDNA
Depositor	Kanemaki, Masato \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

Ordering Please visit Information of Request for Distribution.
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Exclusive MTA [Link]

Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
Please use the Exclusive MTA (Clontech FPs).

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Exclusive MTA [Link]
Remarks	An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required. Please use the Exclusive MTA (Clontech FPs).

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 専用MTA（英語版のみ）[Link]
備考	クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。専用MTA（Clontech FPs, 英語版のみ）をお使いください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13923	pMK280 (mCherry2-NeoR)	DNA solution

cDNA Request [in Japanese] [in English]

References and tips

Electronic file

Featured content	Auxin Inducible Degron (AID) System (English text)
Featured content	Auxin Inducible Degron (AID) System (Japanese text)
Featured content	Genome Editing (English text)
Featured content	Genome Editing (Japanese text)
Featured content	Empty Backbone (English text)
Featured content	Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB13923_A6B8p1_v2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40	RDB13923_A6B8a_2.seq
>D03070A07_A6B8_1_M13(-40)_D02_11.ab1 1 CGCGGACGCG CGTATACGAC TCACTATAGG GCGAATTGGA GCTCGGATCC GGTGCAGGCG 61 CCGCTAGCAT GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC 121 GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG 181 GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC 241 CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG 301 TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAATT 361 GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC 421 TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG 481 GCCCCGTAAT GCAGTGTCGT ACCATGGGCT GGGAGGCCTC CACTGAGCGG ATGTACCCCG 541 AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT 601 ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT 661 ACAACGTCGA CATCAAGTTG GACATCCTTT CCCACAACGA GGACTACACC ATAGATGGAA 721 CAGTACGAAC GCGCCGAGGG CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGTAA 781 TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA 841 AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC 901 TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT 961 AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAT GTATCTACGC 1021 GTAAAATTGG TAAGGCGTTA TATTTTGTTA AATTCGCGTT AAATTTTTGT AATCAGCTCA 1081 TTTTTTACAT AGCGAAATCG CAAATCCTAT ATCAAAGAAT AGACGAGAAT AGGCTTTGAA 1141 GTGTTGTTTC CAAGAATCG //
Primer: SV40pro_F_V2	RDB13923_A6B8b_2.seq
>D03070A07_A6B8_1_SV40pro_F_V2_B10_04.ab1 1 GTAGCACATT TTTGGAGCTA GGCTTTTGCA AGATCGATCA AGAGACAGGA TGAGGATCGT 61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC 121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC 181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG 241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG 301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG 361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG 421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC 481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG 541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC 601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG 661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC 721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC 781 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC 841 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGA AATGACCGAC CAAGCGACGC 901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 961 GAATTCGTTT TCCGGGACGC CGGCTGGATG ATCCCTCCAG CGCGGGGATC TCATGCTGGG 1021 AGTTTCTTCG CCCACCCTAG GGGAGGCTAA CCTGAACACG GGAAAGGAGA CAATACCGGA 1081 AAGAACCGCG CTAATGAACG GCAAATAAAA AAGACAGAAA ATAAAAAACC //
Primer: SV40polyA-R	RDB13923_A6B8c_2.seq
>D03070A07_A6B8_1_SV40polyA-R_G04_19.ab1 1 CCCCATGCAG TTATTAGCAG TAACCTCTAC AATGTGGTAT GGCTGATTAT GATCAGTTAT 61 CTAGATTACT TGTACAGCTC GTCCATGCCG CCGGTGGAGT GGCGGCCCTC GGCGCGTTCG 121 TACTGTTCCA CGATGGTGTA GTCCTCGTTG TGGGAAAGGA TGTCCAACTT GATGTCGACG 181 TTGTAGGCGC CGGGCAGCTG CACGGGCTTC TTGGCCTTGT AGGTGGTCTT GACCTCAGCG 241 TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC CTCTGCTTGA TCTCGCCCTT CAGGGCGCCG 301 TCCTCGGGGT ACATCCGCTC AGTGGAGGCC TCCCAGCCCA TGGTACGACA CTGCATTACG 361 GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC AGCTTCACCT TGTAGATGAA CTCGCCGTCC 421 TGCAGGGAGG AGTCCTGGGT CACGGTCACC ACGCCGCCGT CCTCGAAGTT CATCACGCGC 481 TCCCAATTGA AGCCCTCGGG GAAGGACAGC TTCAAGTAGT CGGGGATGTC GGCGGGGTGC 541 TTCACGTAGG CCTTGGAGCC GTACATGAAC TGAGGGGACA GGATGTCCCA GGCGAAGGGC 601 AGGGGGCCAC CCTTGGTCAC CTTCAGCTTG GCGGTCTGGG TGCCCTCGTA GGGGCGGCCC 661 TCGCCCTCGC CCTCGATCTC GAACTCGTGG CCGTTCACGG AGCCCTCCAT GTGCACCTTG 721 AAGCGCATGA ACTCCTTGAA TGATGGCCAT GTTATCCTCC TCGCCCTTGC TCACCATGCT 781 AGCGGCGCCT GCACCGGATC CGAGCTCCAA TTCGCCCTAT AAGTGAGTCG TATTACGCGC 841 GCTCACTGGC GGTCGTTTTA CAACGTCGTG ACTGGGAAAA AACCCTGGCG TTACCCAAAC 901 TTAAATCGCC TTGCAGCACA TCCCCCCTTT CGCCAGCTGG CGTAATAAGC GAAGAGGCCC 961 GCACCGATCG CCCTTCCCAA CAAATTGCGC AGCCCTGAAT GGCCAAATGG AAAATTGTAA 1021 AGCGGTAAAT ATTTTGGTTA AAAAATCCCG CGAT //
Primer: M13R	RDB13923_A6B8d_2.seq
>D03070A07_A6B8_1_M13R_D01_10.ab1 1 CATCGCGCAA TTACCCCTCA CTAAGGGAAC AAAAGCTGGG TACCGGGCCC CCCCTCGAGG 61 GATCCAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG 121 TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA 181 ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC 241 CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG 301 GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG 361 GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG 421 GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG 481 ATAGCGGTCC GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC 541 CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG 601 CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC 661 CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG 721 TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC 781 ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC 841 CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCAACAG 901 CTGCGCAAGG AAACGCCCGT CGTGGCCCAG CCACGATAAG CCCGCGCTGC CTCGTCTTGC 961 AGTTTCATTC AAGGGCACCG GACAGGGTCG GGTCTTGAAC AAAAAAGAAC CGGGCGCTCC 1021 CTGCCGCTGA CAGCCCGAAC AACGGCGGGC AATCAGAGCA AGCCGAATTG GTCTTGTTTG 1081 TTGCCCAGTT CAATAGCCCG GAATAGCCTC CTCAACCCAA AGCGGTCTGG AAGATCCTGC 1141 TTGCAAATCC ATTCTTTGTT TTCAATTCTC AATGTCCCAG //

Please visit Sequencing and PCR primers for primer information.

References

original	Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl