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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAY19

TOL2 expression vector of OsTIR1(F74A) and mAID-EGFP-NLS marker.

Catalog number RDB18335
Resource name pAY19
Alternative name OsTIR1(F74A)mAID-EGFP-NLS
Clone info. TOL2 expression vector of OsTIR1(F74A) and mAID-EGFP-NLS marker, see Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone pT2AL200R175-CAGGS-EGFP (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell).
Gene/insert name Oryza sativa OsTIR1 cDNA Arabidopsis thaliana IAA17 cDNA Aequorea victoria GFP (EGFP) cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Oryza sativa OsTIR1 Arabidopsis thaliana IAA17 Aequorea victoria GFP (EGFP)

        Reference sequence
          
         
        Sequence (full) RDB18335zzk01.seq provided by the depositor

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
        Additional terms and conditions for distribution The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Koichi Kawakami of the National Institute of Genetics (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004))is requested.
        Ordering Please visit Information of Request for Distribution.[link] 
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        提供案内 (日本国内) [open/close]

        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
        付加的提供条件 Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。利用者は、研究成果の公表にあたって国立遺伝学研究所 川上浩一博士が指定する文献 (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004)) の引用を必要とする。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB18335 pAY19 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Original reference

        original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Auxin Inducible Degron (AID) System (English text)
        Featured content Auxin Inducible Degron (AID) System (Japanese text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB18335_B0Gfp1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: pAxCA_F1
        Region: insert 5'(OsTIR1(F74A) 5')
        Sequence file: RDB18335_B0Gfa.seq check
        >18335_18335_B0Gf_3_pAxCA_F1_C03_09_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN CCTTCTTCTT TTTCCTACNG CTCCTGGGCA ACGTGCTGGT
           61 TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCGAGCTC GGTACCTCGC GAATGCATCT
          121 AGATAGCGCT GCCACCATGA CATACTTTCC TGAAGAGGTC GTCGAACACA TTTTTAGCTT
          181 CCTGCCTGCA CAGAGAGATA GAAACACAGT GAGCCTGGTC TGCAAAGTGT GGTACGAGAT
          241 CGAACGCCTG AGCCGGAGAG GAGTGTTCGT CGGCAACTGC TATGCTGTGA GAGCAGGCAG
          301 GGTCGCCGCT AGGTTTCCAA ATGTGCGCGC ACTGACCGTC AAGGGGAAAC CCCACGCCGC
          361 CGACTTTAAC CTGGTGCCCC CTGATTGGGG AGGATACGCC GGCCCTTGGA TCGAGGCAGC
          421 CGCTCGCGGC TGTCATGGAC TGGAGGAACT GCGCATGAAG CGAATGGTGG TCTCTGACGA
          481 AAGTCTGGAG CTGCTGGCTC GGAGCTTCCC TAGGTTTCGC GCACTGGTGC TGATTTCTTG
          541 CGAAGGCTTC AGCACCGATG GACTGGCAGC CGTGGCCTCC CACTGTAAGC TGCTGCGGGA
          601 GCTGGACCTC CNGGANAATG AAGTGGANGA NAGAGGNCCC AGANGGCTGT CTTGCTTCCC
          661 NNANTCNTGT ACNNGCCNNG NGTCCNGNAC TTTGNNNGNN NCAANNNNAA NNGNATGNTG
        721 GGNNCNTGNN NCNNCNNGNN NNNNNNNN
        //
        Primer: OsTIR1-B_R
        Region: insert mid(OsTIR1(F74A) 3',P2A,mAID,EGFP 5')
        Sequence file: RDB18335_B0Gfb.seq check
        >18335_18335_B0Gf_3_OsTIR1-B_R_D03_12_ABI24.ab1
            1 NNNNNNNNNN NNNNNNGGAN GANNNCATGG CGACCTGCCN AAGGTGGAGA AACTGTATGT
           61 GTATCGGACC ACCGCAGGGG CAAGAGATGA TGCTCCCAAC TTTGTGAAGA TTCTGAGATC
          121 AGGAAGCGGA GCTACTAACT TCAGCCTGCT GAAGCAGGCT GGAGACGTGG AGGAGAACCC
          181 TGGACCTAGA TCTAAGGAGA AGAGTGCTTG TCCTAAAGAT CCAGCCAAAC CTCCGGCCAA
          241 GGCACAAGTT GTGGGATGGC CACCGGTGAG ATCATACCGG AAGAACGTGA TGGTTTCCTG
          301 CCAAAAATCA AGCGGTGGCC CGGAGGCGGC GGCGTTCGTG AAGGTATCAA TGGACGGAGC
          361 ACCGTACTTG AGGAAAATCG ATTTGAGGAT GTATAAAGCT AGCATGGTGA GCAAGGGCGA
          421 GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA
          481 CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA
          541 GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGNCCC ACCCTCGTGA CCACCCTGAC
          601 CTACNGCGTG CAGTGCTTCN GCCGCTACCC CGACCACATG AAGCAGCNCG ACTTCNTNNA
          661 GTCCGCCNTG CCCNANNNTA CNTNCAGGAN CGCACCATCN NCTNNNNNNN NNANNNNNTA
        721 CNNNANCNNN NNNNANNNNN NNNNN
        //
        Primer: EGFP-C2
        Region: insert mid(EGFP 3',Linker,NLS,SV40 pA,loxP,PGK pro)
        Sequence file: RDB18335_B0Gfc.seq check
        >18335_18335_B0Gf_3_EGFP-C2_C02_08_ABI24.ab1
            1 NNNNNNNNNN NNCGCGANCN CNTGGTCCTG CTGGANTTCG TGACCGCCGC CGGGATCACT
           61 CTCGGCATGG ACGAGCTGTA CAAGGGTGCA GGCGCCCAAG CTTCGAATTC TGCAGTCGAC
          121 CCCAAGAAGA AAAGAAAGGT CATCGACGGT ACCGCGGGCC CGGGATCCAC CGGATCTAGA
          181 TAACTGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC
          241 CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT
          301 ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA
          361 TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAAGCGATC
          421 ATATTCAATA ACCCTTAATA TAACTTCGTA TAATGTATGC TATACGAAGT TATTAGGTCT
          481 GAAGAGGAGT TTACGTCCAG CCAAGCTTAG GATCTCGACC TCGAAATTCT ACCGGGTAGG
          541 GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC ATGCGCTTTA GCAGCCCCGC TGGGCACTTG
          601 GCGCTACACA AGTGGCCTCT GGCCTCGCAC ACATTCNNCA TCNACCGGTA NGCGCCNNCC
          661 GANTCCGTNC NTTGGTGGCC CCNTNNCGCC NNCNTCNACT NNTCCCNNAN NCNGGANNNT
        721 NCCCCCCNNN CCNNNNNNNN NN
        //
        Primer: pUC-M13_-100
        Region: T7 pro,Tol2R175,insert 3'(loxP, mPGK ter)
        Sequence file: RDB18335_B0Gfd.seq check
        >18335_18335_B0Gf_3_pUC-M13_-100_E02_14_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNG GNANGCCNGG GTTTTCCCNG TCACGACGTT GTAAAACGAC
           61 GGCCAGTGAG CGCGCGTAAT ACGACTCACT ATAGGGCGAA TTGGGTACCG GCATATGGTT
          121 CTTGACAGAG GTGTAAAAAG TACTCAAAAA TTTTACTCAA GTGAAAGTAC AAGTACTTAG
          181 GGAAAATTTT ACTCAATTAA AAGTAAAAGT ATCTGGCTAG AATCTTACTT GAGTAAAAGT
          241 AAAAAAGTAC TCCATTAAAA TTGTACTTGA GTATTAAGGA AGTAAAAGTA AAAGCAAGAA
          301 AGATCTTAGT GAACCTCTTC GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG
          361 TTATATTAAG GGTTCCGGAT CTCGACCAGC TTCTGATGGA ATTAGAACTT GGCAAAACAA
          421 TACTGAGAAT GAAGTGTATG TGGAACAGAG GCTGCTGATC TCGTTCTTCA GGCTATGAAA
          481 CTGACACATT TGGAAACCAC AGTACTTAGA ACCACAAAGT GGGAATCAAG AGAAAAACAA
          541 TGATCCCACG AGAGATCTAT AGATCTATAG ATCATGAGTG GGAGGAANGA GCTGGCCCTT
          601 AATTTGNTTT TGCTTGTTTA AATTATGANA TCCNACTATG AAACATTATC ATAAAGCAAT
          661 AGTAAAGAGC NNTNAGTAAA NAGCNNNNTT TATCNNATCC CNCCCNNCCC NNNCCCNNAN
        721 CTNNNNNCNN NNNNNNNN
        //
        Primer: PGKpro_F
        Region: insert mid(PuroR)
        Sequence file: RDB18335_B0Gfe.seq check
        >18335_18335_B0Gf_3_PKGpro_F_F02_17_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNTT NNNNNTCTCC GGGCCTTTCG ACCTGCATCC NTCTAGATCT
           61 CGAGCAGCTG AAGCTTACCA TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA
          121 CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG
          181 CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT
          241 CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC
          301 GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG
          361 CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC
          421 GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA
          481 CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC
          541 CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCACAAC CTCCCCTTCT ACGAGCGGCT
          601 CGGCTTCANN GTCACCGCCG ANGTCGAGGT GCCCGANNGN NCNNGCACCT GGTGNNTGNN
          661 CCNNNNGCCC GGTGCCTGAN GCCCGCCNCA NGANCCGNNN CGCCCGANCN AANNNANNNC
        721 NNNNNNCCCN NGCATCNNNN NNNNNN
        //
        Primer: reverse-2
        Region: T3 pro,Tol2L200, CMV en 5'
        Sequence file: RDB18335_B0Gff.seq check
        >18335_18335_B0Gf_3_reverse-2_D02_11_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNGC GCANTTAACC CTCACTAAAG GGAACAAAAG CTGGAGCTCG
           61 GATCTGCTGG GCTTGCTGAA GGTAGGGGGT CAAGAACCAG AGGTGTAAAG TACTTGAGTA
          121 ATTTTACTTG ATTACTGTAC TTAAGTATTA TTTTTGGGGA TTTTTACTTT ACTTGAGTAC
          181 AATTAAAAAT CAATACTTTT ACTTTTACTT AATTACATTT TTTTAGAAAA AAAAGTACTT
          241 TTTACTCCTT ACAATTTTAT TTACAGTCAA AAAGTACTTA TTTTTTGGAG ATCACTTGGG
          301 CCCTCTAGAT GCATGCTCGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG
          361 GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC
          421 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA
          481 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG
          541 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG
          601 ACGGTAAATG GCCCNNCTGG CATTATGCCC AGTACATGAC CTTATGGGNN TTTCCNACNN
          661 NGNANTACAT CTACGTATTA NTCATCGCTA TTANNNNGGT CNANNTGANC CCCACGTTCT
        721 GCTTNNNTCT NCCNNNNNNC CCCCCCCNNN NNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

        2022.08.12

        GNP_filter3_RDBDEP_html_220812.pl