RDB18335 - pAY19

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pAY19 (#RDB18335)

TOL2 expression vector of OsTIR1(F74A) and mAID-EGFP-NLS marker.

Alternative name	OsTIR1(F74A)mAID-EGFP-NLS
Clone info.	TOL2 expression vector of OsTIR1(F74A) and mAID-EGFP-NLS marker, see Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone	pT2AL200R175-CAGGS-EGFP (plasmid)
Selectable markers	Ampicillin (E. coli), Puromycin (mammalian cell).
Gene/insert name	Oryza sativa OsTIR1 cDNA Arabidopsis thaliana IAA17 cDNA Aequorea victoria GFP (EGFP) cDNA
Depositor\|Developer	Kanemaki, Masato \|

Sequence (full)	RDB18335zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
Additional terms and conditions:
The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Koichi Kawakami of the National Institute of Genetics (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004))is requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)). Additional terms and conditions: The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Koichi Kawakami of the National Institute of Genetics (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004))is requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。 MTAに書く付加的使用条件： Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。利用者は、研究成果の公表にあたって国立遺伝学研究所川上浩一博士が指定する文献 (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004)) の引用を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
MTAに書く付加的使用条件：
Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。利用者は、研究成果の公表にあたって国立遺伝学研究所川上浩一博士が指定する文献 (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004)) の引用を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18335	pAY19	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAY19 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18335).

Reference section:

Yesbolatova, A., Saito, Y., Kitamoto, N., Makino-Itou, H., Ajima, R., Nakano, R., Nakaoka, H., Fukui, K., Gamo, K., Tominari, Y., Takeuchi, H., Saga, Y., Hayashi, K., Kanemaki, M.T., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18335_B0Gfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164) Region: insert 5'(OsTIR1(F74A) 5') Sequence file: RDB18335_B0Gfa.seq
>18335_18335_B0Gf_3_pAxCA_F1_C03_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN CCTTCTTCTT TTTCCTACNG CTCCTGGGCA ACGTGCTGGT 61 TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCGAGCTC GGTACCTCGC GAATGCATCT 121 AGATAGCGCT GCCACCATGA CATACTTTCC TGAAGAGGTC GTCGAACACA TTTTTAGCTT 181 CCTGCCTGCA CAGAGAGATA GAAACACAGT GAGCCTGGTC TGCAAAGTGT GGTACGAGAT 241 CGAACGCCTG AGCCGGAGAG GAGTGTTCGT CGGCAACTGC TATGCTGTGA GAGCAGGCAG 301 GGTCGCCGCT AGGTTTCCAA ATGTGCGCGC ACTGACCGTC AAGGGGAAAC CCCACGCCGC 361 CGACTTTAAC CTGGTGCCCC CTGATTGGGG AGGATACGCC GGCCCTTGGA TCGAGGCAGC 421 CGCTCGCGGC TGTCATGGAC TGGAGGAACT GCGCATGAAG CGAATGGTGG TCTCTGACGA 481 AAGTCTGGAG CTGCTGGCTC GGAGCTTCCC TAGGTTTCGC GCACTGGTGC TGATTTCTTG 541 CGAAGGCTTC AGCACCGATG GACTGGCAGC CGTGGCCTCC CACTGTAAGC TGCTGCGGGA 601 GCTGGACCTC CNGGANAATG AAGTGGANGA NAGAGGNCCC AGANGGCTGT CTTGCTTCCC 661 NNANTCNTGT ACNNGCCNNG NGTCCNGNAC TTTGNNNGNN NCAANNNNAA NNGNATGNTG 721 GGNNCNTGNN NCNNCNNGNN NNNNNNNN //
Primer: OsTIR1-B_R (Pr1085) Region: insert mid(OsTIR1(F74A) 3',P2A,mAID,EGFP 5') Sequence file: RDB18335_B0Gfb.seq
>18335_18335_B0Gf_3_OsTIR1-B_R_D03_12_ABI24.ab1 1 NNNNNNNNNN NNNNNNGGAN GANNNCATGG CGACCTGCCN AAGGTGGAGA AACTGTATGT 61 GTATCGGACC ACCGCAGGGG CAAGAGATGA TGCTCCCAAC TTTGTGAAGA TTCTGAGATC 121 AGGAAGCGGA GCTACTAACT TCAGCCTGCT GAAGCAGGCT GGAGACGTGG AGGAGAACCC 181 TGGACCTAGA TCTAAGGAGA AGAGTGCTTG TCCTAAAGAT CCAGCCAAAC CTCCGGCCAA 241 GGCACAAGTT GTGGGATGGC CACCGGTGAG ATCATACCGG AAGAACGTGA TGGTTTCCTG 301 CCAAAAATCA AGCGGTGGCC CGGAGGCGGC GGCGTTCGTG AAGGTATCAA TGGACGGAGC 361 ACCGTACTTG AGGAAAATCG ATTTGAGGAT GTATAAAGCT AGCATGGTGA GCAAGGGCGA 421 GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA 481 CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA 541 GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGNCCC ACCCTCGTGA CCACCCTGAC 601 CTACNGCGTG CAGTGCTTCN GCCGCTACCC CGACCACATG AAGCAGCNCG ACTTCNTNNA 661 GTCCGCCNTG CCCNANNNTA CNTNCAGGAN CGCACCATCN NCTNNNNNNN NNANNNNNTA 721 CNNNANCNNN NNNNANNNNN NNNNN //
Primer: EGFP-C2 (Pr0402) Region: insert mid(EGFP 3',Linker,NLS,SV40 pA,loxP,PGK pro) Sequence file: RDB18335_B0Gfc.seq
>18335_18335_B0Gf_3_EGFP-C2_C02_08_ABI24.ab1 1 NNNNNNNNNN NNCGCGANCN CNTGGTCCTG CTGGANTTCG TGACCGCCGC CGGGATCACT 61 CTCGGCATGG ACGAGCTGTA CAAGGGTGCA GGCGCCCAAG CTTCGAATTC TGCAGTCGAC 121 CCCAAGAAGA AAAGAAAGGT CATCGACGGT ACCGCGGGCC CGGGATCCAC CGGATCTAGA 181 TAACTGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC 241 CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT 301 ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA 361 TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAAGCGATC 421 ATATTCAATA ACCCTTAATA TAACTTCGTA TAATGTATGC TATACGAAGT TATTAGGTCT 481 GAAGAGGAGT TTACGTCCAG CCAAGCTTAG GATCTCGACC TCGAAATTCT ACCGGGTAGG 541 GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC ATGCGCTTTA GCAGCCCCGC TGGGCACTTG 601 GCGCTACACA AGTGGCCTCT GGCCTCGCAC ACATTCNNCA TCNACCGGTA NGCGCCNNCC 661 GANTCCGTNC NTTGGTGGCC CCNTNNCGCC NNCNTCNACT NNTCCCNNAN NCNGGANNNT 721 NCCCCCCNNN CCNNNNNNNN NN //
Primer: pUC-M13_-100 (Pr0310) Region: T7 pro,Tol2R175,insert 3'(loxP, mPGK ter) Sequence file: RDB18335_B0Gfd.seq
>18335_18335_B0Gf_3_pUC-M13_-100_E02_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNG GNANGCCNGG GTTTTCCCNG TCACGACGTT GTAAAACGAC 61 GGCCAGTGAG CGCGCGTAAT ACGACTCACT ATAGGGCGAA TTGGGTACCG GCATATGGTT 121 CTTGACAGAG GTGTAAAAAG TACTCAAAAA TTTTACTCAA GTGAAAGTAC AAGTACTTAG 181 GGAAAATTTT ACTCAATTAA AAGTAAAAGT ATCTGGCTAG AATCTTACTT GAGTAAAAGT 241 AAAAAAGTAC TCCATTAAAA TTGTACTTGA GTATTAAGGA AGTAAAAGTA AAAGCAAGAA 301 AGATCTTAGT GAACCTCTTC GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG 361 TTATATTAAG GGTTCCGGAT CTCGACCAGC TTCTGATGGA ATTAGAACTT GGCAAAACAA 421 TACTGAGAAT GAAGTGTATG TGGAACAGAG GCTGCTGATC TCGTTCTTCA GGCTATGAAA 481 CTGACACATT TGGAAACCAC AGTACTTAGA ACCACAAAGT GGGAATCAAG AGAAAAACAA 541 TGATCCCACG AGAGATCTAT AGATCTATAG ATCATGAGTG GGAGGAANGA GCTGGCCCTT 601 AATTTGNTTT TGCTTGTTTA AATTATGANA TCCNACTATG AAACATTATC ATAAAGCAAT 661 AGTAAAGAGC NNTNAGTAAA NAGCNNNNTT TATCNNATCC CNCCCNNCCC NNNCCCNNAN 721 CTNNNNNCNN NNNNNNNN //
Primer: PGKpro_F (Pr0249) Region: insert mid(PuroR) Sequence file: RDB18335_B0Gfe.seq
>18335_18335_B0Gf_3_PKGpro_F_F02_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNTT NNNNNTCTCC GGGCCTTTCG ACCTGCATCC NTCTAGATCT 61 CGAGCAGCTG AAGCTTACCA TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA 121 CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG 181 CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT 241 CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC 301 GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG 361 CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC 421 GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA 481 CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC 541 CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCACAAC CTCCCCTTCT ACGAGCGGCT 601 CGGCTTCANN GTCACCGCCG ANGTCGAGGT GCCCGANNGN NCNNGCACCT GGTGNNTGNN 661 CCNNNNGCCC GGTGCCTGAN GCCCGCCNCA NGANCCGNNN CGCCCGANCN AANNNANNNC 721 NNNNNNCCCN NGCATCNNNN NNNNNN //
Primer: reverse-2 (Pr0100) Region: T3 pro,Tol2L200, CMV en 5' Sequence file: RDB18335_B0Gff.seq
>18335_18335_B0Gf_3_reverse-2_D02_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGC GCANTTAACC CTCACTAAAG GGAACAAAAG CTGGAGCTCG 61 GATCTGCTGG GCTTGCTGAA GGTAGGGGGT CAAGAACCAG AGGTGTAAAG TACTTGAGTA 121 ATTTTACTTG ATTACTGTAC TTAAGTATTA TTTTTGGGGA TTTTTACTTT ACTTGAGTAC 181 AATTAAAAAT CAATACTTTT ACTTTTACTT AATTACATTT TTTTAGAAAA AAAAGTACTT 241 TTTACTCCTT ACAATTTTAT TTACAGTCAA AAAGTACTTA TTTTTTGGAG ATCACTTGGG 301 CCCTCTAGAT GCATGCTCGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG 361 GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC 421 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA 481 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG 541 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG 601 ACGGTAAATG GCCCNNCTGG CATTATGCCC AGTACATGAC CTTATGGGNN TTTCCNACNN 661 NGNANTACAT CTACGTATTA NTCATCGCTA TTANNNNGGT CNANNTGANC CCCACGTTCT 721 GCTTNNNTCT NCCNNNNNNC CCCCCCCNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]