Sequencing and PCR primers for primer information.
A plasmid for construction of C-terminal mCherry2-BSD tagging donors.
| Catalog number | RDB16817 |
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| Resource name | pMK389 (mCherry2-BSD) |
| Clone info. | A plasmid for construction of C-terminal mCherry2-BSD tagging donors. |
| Vector backbone | pBluescript (plasmid) |
| Size of vector backbone | 2.9 kb |
| Selectable markers | Am^r (E. coli) |
| Gene/insert name | Discosoma sp. RFP cDNA |
| Depositor | Kanemaki, Masato | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Exclusive MTA [Link] |
| Remarks | The BIOLOGICAL RESOURCE may not be used for COMMERCIAL PURPOSES. Please read the Exclusive MTA (Clontech FPs). |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB16817 | pMK389 (mCherry2-BSD) | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
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| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB16817_A8Lpp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pUC-M13_-100 | RDB16817_A8Lpa.seq |
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>D06157B4_A8LP_2_pUC-M13_-100_E06_15_ABI24.ab1 1 GAAGGTCAAG CGATTAGGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG 61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CGGATCCGGT 121 GCAGGCGCCG CTAGCATGGT GAGCAAGGGC GAGGAGGATA ACATGGCCAT CATCAAGGAG 181 TTCATGCGCT TCAAGGTGCA CATGGAGGGC TCCGTGAACG GCCACGAGTT CGAGATCGAG 241 GGCGAGGGCG AGGGCCGCCC CTACGAGGGC ACCCAGACCG CCAAGCTGAA GGTGACCAAG 301 GGTGGCCCCC TGCCCTTCGC CTGGGACATC CTGTCCCCTC AGTTCATGTA CGGCTCCAAG 361 GCCTACGTGA AGCACCCCGC CGACATCCCC GACTACTTGA AGCTGTCCTT CCCCGAGGGC 421 TTCAATTGGG AGCGCGTGAT GAACTTCGAG GACGGCGGCG TGGTGACCGT GACCCAGGAC 481 TCCTCCCTGC AGGACGGCGA GTTCATCTAC AAGGTGAAGC TGCGCGGCAC CAACTTCCCC 541 TCCGACGGCC CCGTAATGCA GTGTCGTACC ATGGGCTGGG AGGCCTCCAC TGAGCGGATG 601 TACCCCGAGG ACGGCGCCCT GAAGGGCGAG ATCAAGCAGA GGCTGAAGCT GAAGGACGGC 661 GGCCACTACG ACGCTGAGGT CAAGACCACC TACAAGGCCA AGAAGCCCGT GCAGCTGCCC 721 GGCGCCTACA ACGTCGACAT CAAGTTGGAC ATCCTTTCCC ACAACGAGGA CTACACCATC 781 GTGGAACAGT ACGAACGCGC CGAGGGCCGC CACTCCACCG GCGGCATGGA CGAGCTGTAC 841 AAGTAATCTA GATAACTGAT CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT 901 TTAAAAAAAC CTCCCACACC TCCCCCCTGA AACCTGAAAC ATAAAAATGA AATGCAATTT 961 GTTGTTTGTT TAACTTTGTT TATTTGCAGC TTTATAAATG ATTTACAAAT AAAGCAAATT 1021 AGCATCACAA TTTCACAAAT AAGCCAATTT TTTTTTCACT GCATTTCTAG TTGTGCTTGT 1081 CCAAAACCTC ATCCAACTGT AATTCCGTA // |
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| Primer: reverse | RDB16817_A8Lpb.seq |
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>D06157B4_A8LP_2_reverse_F06_18_ABI24.ab1 1 CTAGCAAATT ACCCCTCACT AAAGGGAACA AAAGCTGGGT ACCGGATCCT AGTGAACCTC 61 TTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATATT AAGGGTTCCG 121 GATCTCGACC AGCTTCTGAT GGAATTAGAA CTTGGCAAAA CAATACTGAG AATGAAGTGT 181 ATGTGGAACA GAGGCTGCTG ATCTCGTTCT TCAGGCTATG AAACTGACAC ATTTGGAAAC 241 CACAGTACTT AGAACCACAA AGTGGGAATC AAGAGAAAAA CAATGATCCC ACGAGAGATC 301 TATAGATCTA TAGATCATGA GTGGGAGGAA TGAGCTGGCC CTTAATTTGG TTTTGCTTGT 361 TTAAATTATG ATATCCAACT ATGAAACATT ATCATAAAGC AATAGTAAAG AGCCTTCAGT 421 AAAGAGCAGG CATTTATCTA ATCCCACCCC ACCCCCACCC CCGTAGCTCC AATCCTTCCA 481 TTCAAAATGT AGGTACTCTG TTCTCACCCT TCTTAACAAA GTATGACAGG AAAAACTTCC 541 ATTTTAGTGG ACATCTTTAT TGTTTAATAG ATCATCAATT TCTGCATCCC GGGGATCTGA 601 TATCATCGAT TAGCCCTCCC ACACATAACC AGAGGGCAGC AATTCACGAA TCCCAACTGC 661 CGTCGGCTGT CCATCACTGT CCTTCACTAT GGCTTTGATC CCAGGATGCA GATCGAGAAG 721 CACCTGTCGG CACCGTCCGC AGGGGCTCAA GATGCCCCTG TTCTCATTTC CGATCGCGAC 781 GATACAAGTC AGGTTGCCAG CTGCCGCAGC AGCAGCAGTG CCCAGCACCA CGAGTTCTGC 841 ACAAGGTCCC CCCAGTAAAA TGATATACAT TGACACCAGT TGAAGATGCG GCCGTCGCTA 901 GAGAGAGCTG CGCTGGCGAC GCTGTAGTCT TCAGAGATGG GGATGCTGTT GATTGTAGCC 961 GTTGCTCTTT CAATGAGGGT GGATTTCTTC TTGAGACAAA GGCTTGCCCA TTGATGGCTC 1021 GGAGATCTAG AATGAATGCC AAGGTTCGAA CGGCCGAGAG ATTGCAGAAG AAGGAAGTAA 1081 CAGCGCCGGT CAAGACCGAG // |
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| Primer: PGK_N_rev | RDB16817_A8Lpc.seq |
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>D06157B4_A8LP_2_PGK_N_rev_G06_21_ABI24.ab1 1 CGTGGGGCGG CTCCCCTACC CGGTAGATTT CGAGGTCGAG ATCCTAAGCT TGGCTGGACG 61 TAAACTCCTC TTCAGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG 121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA 181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA 241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG 301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG 361 CTGATTATGA TCAGTTATCT AGATTACTTG TACAGCTCGT CCATGCCGCC GGTGGAGTGG 421 CGGCCCTCGG CGCGTTCGTA CTGTTCCACG ATGGTGTAGT CCTCGTTGTG GGAAAGGATG 481 TCCAACTTGA TGTCGACGTT GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT GGCCTTGTAG 541 GTGGTCTTGA CCTCAGCGTC GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT CTGCTTGATC 601 TCGCCCTTCA GGGCGCCGTC CTCGGGGTAC ATCCGCTCAG TGGAGGCCTC CCAGCCCATG 661 GTACGACACT GCATTACGGG GCCGTCGGAG GGGAAGTTGG TGCCGCGCAG CTTCACCTTG 721 TAGATGAACT CGCCGTCCTG CAGGGAGGAG TCCTGGGTCA CGGTCACCAC GCCGCCGTCC 781 TCGAAGTTCA TCACGCGCTC CCAATTGAAG CCCTCGGGGA AGGACAGCTT CAAGTAGTCG 841 GGGATGTCGG CGGGGTGCTT CACGTAGCCT TGGAGCCGTA CATGAACTGA GGGGGACAGG 901 ATGTCCCAGG CGAAGGGCAG GGGGCCCACC CTTGGTCACC CTTCAGCTTG GCGGTCTGGG 961 TGCCCTCGTA GGGCGGGCCC TCGCCCTCGC CCTCGATCTC GAAACTCGTG ACCGGTTTCA 1021 CGGGAGCCCC TCCCATGTGC ACTTTGGAAG CGGCATGAAC TCCGTTGATG AATGACAATG 1081 GTTAATTCAT CTTCGACCCG TGGCCTTCAG A // |
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| Primer: PGKpro_F | RDB16817_A8Lpd.seq |
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>D06157B4_A8LP_2_PGKpro_F_H06_24_ABI24.ab1 1 AAGGGGGGGC GTGTTCTCTC TTCTCATCTC CGGGCCTTTC GACCTGCATC CATCTAGATC 61 TCGAGCCACC ATGGCCAAGC CTTTGTCTCA AGAAGAATCC ACCCTCATTG AAAGAGCAAC 121 GGCTACAATC AACAGCATCC CCATCTCTGA AGACTACAGC GTCGCCAGCG CAGCTCTCTC 181 TAGCGACGGC CGCATCTTCA CTGGTGTCAA TGTATATCAT TTTACTGGGG GACCTTGTGC 241 AGAACTCGTG GTGCTGGGCA CTGCTGCTGC TGCGGCAGCT GGCAACCTGA CTTGTATCGT 301 CGCGATCGGA AATGAGAACA GGGGCATCTT GAGCCCCTGC GGACGGTGCC GACAGGTGCT 361 TCTCGATCTG CATCCTGGGA TCAAAGCCAT AGTGAAGGAC AGTGATGGAC AGCCGACGGC 421 AGTTGGGATT CGTGAATTGC TGCCCTCTGG TTATGTGTGG GAGGGCTAAT CGATGATATC 481 AGATCCCCGG GATGCAGAAA TTGATGATCT ATTAAACAAT AAAGATGTCC ACTAAAATGG 541 AAGTTTTTCC TGTCATACTT TGTTAAGAAG GGTGAGAACA GAGTACCTAC ATTTTGAATG 601 GAAGGATTGG AGCTACGGGG GTGGGGGTGG GGTGGGATTA GATAAATGCC TGCTCTTTAC 661 TGAAGGCTCT TTACTATTGC TTTATGATAA TGTTTCATAG TTGGATATCA TAATTTAAAC 721 AAGCAAAACC AAATTAAGGG CCAGCTCATT CCTCCCACTC ATGATCTATA GATCTATAGA 781 TCTCTCGTGG GATCATTGTT TTTCTCTTGA TTCCCACTTT GTGGTTCTAA GTACTGTGGT 841 TTCCAAATGT GTCAGTTTCA TAGCCTGAAG AACGAAGATC AGCAGCCTCT GTTCCACATA 901 CAACTTCATT CTCAGTATTG TTTTGCCAAG TTCTAATTTC CATCAGAAGC TGGGTCGAGA 961 TCCGGAACCC TTAATATAAA CTTCGTTATA TGTATGCTAT ACGAAGGTTA ATTAAGGTCC 1021 CTCGAAGAGG GTTCCACTTA GGAATCCGGG TACTCAGCCT TTTTGATTCC CTTTAGTGAG 1081 GGATTACTTT GCGCGCTGAC GGTCATCCAT GGGTCAATAG // |
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Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2019.07.12
GNP_filter3_RDBDEP_html_190431.pl