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pMK389 (mCherry2-BSD) (#RDB16817)

A plasmid for construction of C-terminal mCherry2-BSD tagging donors.

Clone info. A plasmid for construction of C-terminal mCherry2-BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Growth conditions LB+Amp, 37oC
Gene/insert name Discosoma sp. RFP cDNA
Depositor|Developer Kanemaki, Masato |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16817 pMK389 (mCherry2-BSD) DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK389 (mCherry2-BSD) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16817).

Reference section:

Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16817_A8Lpp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310)
Region: T7 pro,insert 5'(Linker,mCherry2 5')
Sequence file: RDB16817_A8Lpe.seq check
>D06157B4_A8LP_2_pUC-M13_-100_E06_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT GGNNNNNCCN NGGTTTTCCC NGTCNCGACG TTGTAAAACG
   61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CGGATCCGGT
  121 GCAGGCGCCG CTAGCATGGT GAGCAAGGGC GAGGAGGATA ACATGGCCAT CATCAAGGAG
  181 TTCATGCGCT TCAAGGTGCA CATGGAGGGC TCCGTGAACG GCCACGAGTT CGAGATCGAG
  241 GGCGAGGGCG AGGGCCGCCC CTACGAGGGC ACCCAGACCG CCAAGCTGAA GGTGACCAAG
  301 GGTGGCCCCC TGCCCTTCGC CTGGGACATC CTGTCCCCTC AGTTCATGTA CGGCTCCAAG
  361 GCCTACGTGA AGCACCCCGC CGACATCCCC GACTACTTGA AGCTGTCCTT CCCCGAGGGC
  421 TTCAATTGGG AGCGCGTGAT GAACTTCGAG GACGGCGGCG TGGTGACCGT GACCCAGGAC
  481 TCCTCCCTGC AGGACGGCGA GTTCATCTAC AAGGTGAAGC TGCGCGGCAC CAACTTCCCC
  541 TCCGACGGCC CCGTAATGCA GTGTCGTACC ATGGGCTGGG AGGCCTCCAC TGAGCGGATG
  601 TACCCCGAGG ACGGCGCCCT GAAGGGCGAG ATCAAGCAGA GGCTGAAGCT GAAGGACGGC
  661 GGCCACTACG ACGCTGANGT CAAGANCACC TACAAGGCCN AGAAGCCCGT GCAGCTGCCC
  721 GGCGCCTACA ACGTCGACAT CAAGTTGGAC ATCCTTTCNC ACAACGAGGA CTACACCATC
  781 GNGGAACAGT ACGAACGCGC CGANGGCCGC CACTCCACCG GCGGCATGGA CGANCTGTAC
  841 AAGTAATCTA GATANNTGAT CATAATCAGC NNTACCACAT TTGNAGANGN TTTACTNGCT
  901 TTAAAAAAAC CNNCCNCANC NNCNCCNNGN ANCCNNAAAC NTNAAANNGN ANNGNNNNTN
961 NNNGNTNNNT NNNNNTNNNT NNNTNGNNNN NTNANNANNN NNNNNNN
//
Primer: lacIpro-B_F (Pr0724)
Region: T3 pro,insert 3'(loxP,mPGK ter)
Sequence file: RDB16817_A8Lpf.seq check
>16817_16817_B3C9F_lacIpro-B_F_H01_22_ABI24.ab1
    1 NNNNNNNNNN NNNNGNNCGN NNCGNGTCAG TGAGCGAGGN NNNNNNANNA NNNGCCCAAT
   61 ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT
  121 TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA
  181 GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG
  241 ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCGCG CAATTAACCC
  301 TCACTAAAGG GAACAAAAGC TGGGTACCGG ATCCTAGTGA ACCTCTTCGA GGGACCTAAT
  361 AACTTCGTAT AGCATACATT ATACGAAGTT ATATTAAGGG TTCCGGATCT CGACCAGCTT
  421 CTGATGGAAT TAGAACTTGG CAAAACAATA CTGAGAATGA AGTGTATGTG GAACAGAGGC
  481 TGCTGATCTC GTTCTTCAGG CTATGAAACT GACACATTTG GAAACCACAG TACTTAGAAC
  541 CACAAAGTGG GAATCAAGAG AAAAACAATG ATCCCACGAG AGATCTATAG ATCTATAGAT
  601 CATGAGTGGG AGGAATGAGC TGGNCCTTAA TTTGNTTTTG CTTGTTTAAA TTATGATATC
  661 CAACTATGAA ACATTATCAT AAAGCAATAG TAAAGAGCCT TCNGTAAAGA GCANGNATTT
  721 ATCTAATCNN NNNNNACCCC CACCCCCGTA NCTCCNATCC NTCCNTTNNA AATGNNNNAC
  781 TCTGNTCTCA CCCTTCTTAA CNAAGTATGA NNNGAAAAAC TTNCNTTTTA NNGGNNNTCT
  841 TTATNGNTTA ANANATCATC NNTTTNNGCA TCCCGGGGNN NTGANNTCNN NNANNANNCN
901 TCCNNNNCNT NANNNNANGG NANNNNNTCN NNANNNCCNN NNNNNNNN
//
Primer: PGK_N_rev (Pr0243)
Region: insert mid(loxP,SV40 pA,mCherry2 3')
Sequence file: RDB16817_A8Lpg.seq check
>D06157B4_A8LP_2_PGK_N_rev_G06_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGNANANTT CGAGGTCGAN NTCCTAAGCT TGGCTGGACG
   61 TAAACTCCTC TTCAGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG
  121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA
  181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA
  241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG
  301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
  361 CTGATTATGA TCAGTTATCT AGATTACTTG TACAGCTCGT CCATGCCGCC GGTGGAGTGG
  421 CGGCCCTCGG CGCGTTCGTA CTGTTCCACG ATGGTGTAGT CCTCGTTGTG GGAAAGGATG
  481 TCCAACTTGA TGTCGACGTT GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT GGCCTTGTAG
  541 GTGGTCTTGA CCTCAGCGTC GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT CTGCTTGATC
  601 TCGCCCTTCA GGGCGCCGTC CTCGGGGTAC ATCCGCTCAG TGGANGCCTC CCAGCCCATG
  661 GTACGACACT GCATTACGGG GCCGTCGGAN GGGAAGTTGG TGCCGCGCAN CTTCACCTTG
  721 TAGATGAACT CGCCGTCCTG CANGGAGGAN TCCTGNGTCA CGGTCACCAC GCCGCCGTCC
  781 TCNAAGTTCA TCACGCGCTC CCAATTGAAG CCNTCGGGGA ANGACAGCTT CNAGTANTCN
  841 GGGNNGTCNG CGGGGNGCTT CACGTNNNCT NGGANCCNNA CATGANCTGN NGGGGANAGG
  901 ANGTCCNANG CGAAGGGCNN GGGGNCNACC CNNNNNNNNC NTTCANCTTN NNNGNNNNGN
961 NNNCNNNNNN NNGNNNNNNN NNN
//
Primer: PGKpro_F (Pr0249)
Region: insert mid(BSD,mousePGK ter)
Sequence file: RDB16817_A8Lph.seq check
>D06157B4_A8LP_2_PGKpro_F_H06_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC CGGGCCTTTC GNCCTGCATC CNTCTAGATC
   61 TCGAGCCACC ATGGCCAAGC CTTTGTCTCA AGAAGAATCC ACCCTCATTG AAAGAGCAAC
  121 GGCTACAATC AACAGCATCC CCATCTCTGA AGACTACAGC GTCGCCAGCG CAGCTCTCTC
  181 TAGCGACGGC CGCATCTTCA CTGGTGTCAA TGTATATCAT TTTACTGGGG GACCTTGTGC
  241 AGAACTCGTG GTGCTGGGCA CTGCTGCTGC TGCGGCAGCT GGCAACCTGA CTTGTATCGT
  301 CGCGATCGGA AATGAGAACA GGGGCATCTT GAGCCCCTGC GGACGGTGCC GACAGGTGCT
  361 TCTCGATCTG CATCCTGGGA TCAAAGCCAT AGTGAAGGAC AGTGATGGAC AGCCGACGGC
  421 AGTTGGGATT CGTGAATTGC TGCCCTCTGG TTATGTGTGG GAGGGCTAAT CGATGATATC
  481 AGATCCCCGG GATGCAGAAA TTGATGATCT ATTAAACAAT AAAGATGTCC ACTAAAATGG
  541 AAGTTTTTCC TGTCATACTT TGTTAAGAAG GGTGAGAACA GAGTACCTAC ATTTTGAATG
  601 GAAGGATTGG AGCTACGGGG GTGGGGGTGG GGTGGGATTA GATAAATGCC TGCTCTTTAC
  661 TGAAGGCTCT TTACTATTGC TTTATGATAA TGTTTCATAG TTGGATATCA TAATTTAAAC
  721 AAGCAAAACC AAATTAAGGG CCAGCTCATT CCTCNNANTC ATGATCTATA GATCTATAGA
  781 TCTCTCGTGN GATCATTGTT TTTCTCTTGA TTCCCACTTT GTGGTTCTAA NTACTGNGGT
  841 TTCCAAATGT GTCNGTTNCA TAGCNTGAAN NNNNNAGATC AGCANCCTCN GNTNCACATA
901 CNNNTTCNTN NNCNGTATTG NTTTGNCAAN TNNNNANTNC NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]