Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK389 (mCherry2-BSD)

A plasmid for construction of C-terminal mCherry2-BSD tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose)[Link]
Remarks	The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose).

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA (Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用)[Link]
備考	本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。専用MTA（Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用）をご参照ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16817_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 Sequence file: RDB16817_A8Lpa.seq
>D06157B4_A8LP_2_pUC-M13_-100_E06_15_ABI24.ab1     1 GAAGGTCAAG CGATTAGGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG    61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CGGATCCGGT   121 GCAGGCGCCG CTAGCATGGT GAGCAAGGGC GAGGAGGATA ACATGGCCAT CATCAAGGAG   181 TTCATGCGCT TCAAGGTGCA CATGGAGGGC TCCGTGAACG GCCACGAGTT CGAGATCGAG   241 GGCGAGGGCG AGGGCCGCCC CTACGAGGGC ACCCAGACCG CCAAGCTGAA GGTGACCAAG   301 GGTGGCCCCC TGCCCTTCGC CTGGGACATC CTGTCCCCTC AGTTCATGTA CGGCTCCAAG   361 GCCTACGTGA AGCACCCCGC CGACATCCCC GACTACTTGA AGCTGTCCTT CCCCGAGGGC   421 TTCAATTGGG AGCGCGTGAT GAACTTCGAG GACGGCGGCG TGGTGACCGT GACCCAGGAC   481 TCCTCCCTGC AGGACGGCGA GTTCATCTAC AAGGTGAAGC TGCGCGGCAC CAACTTCCCC   541 TCCGACGGCC CCGTAATGCA GTGTCGTACC ATGGGCTGGG AGGCCTCCAC TGAGCGGATG   601 TACCCCGAGG ACGGCGCCCT GAAGGGCGAG ATCAAGCAGA GGCTGAAGCT GAAGGACGGC   661 GGCCACTACG ACGCTGAGGT CAAGACCACC TACAAGGCCA AGAAGCCCGT GCAGCTGCCC   721 GGCGCCTACA ACGTCGACAT CAAGTTGGAC ATCCTTTCCC ACAACGAGGA CTACACCATC   781 GTGGAACAGT ACGAACGCGC CGAGGGCCGC CACTCCACCG GCGGCATGGA CGAGCTGTAC   841 AAGTAATCTA GATAACTGAT CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT   901 TTAAAAAAAC CTCCCACACC TCCCCCCTGA AACCTGAAAC ATAAAAATGA AATGCAATTT   961 GTTGTTTGTT TAACTTTGTT TATTTGCAGC TTTATAAATG ATTTACAAAT AAAGCAAATT  1021 AGCATCACAA TTTCACAAAT AAGCCAATTT TTTTTTCACT GCATTTCTAG TTGTGCTTGT 1081 CCAAAACCTC ATCCAACTGT AATTCCGTA //
Primer: reverse Sequence file: RDB16817_A8Lpb.seq
>D06157B4_A8LP_2_reverse_F06_18_ABI24.ab1     1 CTAGCAAATT ACCCCTCACT AAAGGGAACA AAAGCTGGGT ACCGGATCCT AGTGAACCTC    61 TTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATATT AAGGGTTCCG   121 GATCTCGACC AGCTTCTGAT GGAATTAGAA CTTGGCAAAA CAATACTGAG AATGAAGTGT   181 ATGTGGAACA GAGGCTGCTG ATCTCGTTCT TCAGGCTATG AAACTGACAC ATTTGGAAAC   241 CACAGTACTT AGAACCACAA AGTGGGAATC AAGAGAAAAA CAATGATCCC ACGAGAGATC   301 TATAGATCTA TAGATCATGA GTGGGAGGAA TGAGCTGGCC CTTAATTTGG TTTTGCTTGT   361 TTAAATTATG ATATCCAACT ATGAAACATT ATCATAAAGC AATAGTAAAG AGCCTTCAGT   421 AAAGAGCAGG CATTTATCTA ATCCCACCCC ACCCCCACCC CCGTAGCTCC AATCCTTCCA   481 TTCAAAATGT AGGTACTCTG TTCTCACCCT TCTTAACAAA GTATGACAGG AAAAACTTCC   541 ATTTTAGTGG ACATCTTTAT TGTTTAATAG ATCATCAATT TCTGCATCCC GGGGATCTGA   601 TATCATCGAT TAGCCCTCCC ACACATAACC AGAGGGCAGC AATTCACGAA TCCCAACTGC   661 CGTCGGCTGT CCATCACTGT CCTTCACTAT GGCTTTGATC CCAGGATGCA GATCGAGAAG   721 CACCTGTCGG CACCGTCCGC AGGGGCTCAA GATGCCCCTG TTCTCATTTC CGATCGCGAC   781 GATACAAGTC AGGTTGCCAG CTGCCGCAGC AGCAGCAGTG CCCAGCACCA CGAGTTCTGC   841 ACAAGGTCCC CCCAGTAAAA TGATATACAT TGACACCAGT TGAAGATGCG GCCGTCGCTA   901 GAGAGAGCTG CGCTGGCGAC GCTGTAGTCT TCAGAGATGG GGATGCTGTT GATTGTAGCC   961 GTTGCTCTTT CAATGAGGGT GGATTTCTTC TTGAGACAAA GGCTTGCCCA TTGATGGCTC  1021 GGAGATCTAG AATGAATGCC AAGGTTCGAA CGGCCGAGAG ATTGCAGAAG AAGGAAGTAA 1081 CAGCGCCGGT CAAGACCGAG //
Primer: PGK_N_rev Sequence file: RDB16817_A8Lpc.seq
>D06157B4_A8LP_2_PGK_N_rev_G06_21_ABI24.ab1     1 CGTGGGGCGG CTCCCCTACC CGGTAGATTT CGAGGTCGAG ATCCTAAGCT TGGCTGGACG    61 TAAACTCCTC TTCAGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG   121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA   181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA   241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG   301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG   361 CTGATTATGA TCAGTTATCT AGATTACTTG TACAGCTCGT CCATGCCGCC GGTGGAGTGG   421 CGGCCCTCGG CGCGTTCGTA CTGTTCCACG ATGGTGTAGT CCTCGTTGTG GGAAAGGATG   481 TCCAACTTGA TGTCGACGTT GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT GGCCTTGTAG   541 GTGGTCTTGA CCTCAGCGTC GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT CTGCTTGATC   601 TCGCCCTTCA GGGCGCCGTC CTCGGGGTAC ATCCGCTCAG TGGAGGCCTC CCAGCCCATG   661 GTACGACACT GCATTACGGG GCCGTCGGAG GGGAAGTTGG TGCCGCGCAG CTTCACCTTG   721 TAGATGAACT CGCCGTCCTG CAGGGAGGAG TCCTGGGTCA CGGTCACCAC GCCGCCGTCC   781 TCGAAGTTCA TCACGCGCTC CCAATTGAAG CCCTCGGGGA AGGACAGCTT CAAGTAGTCG   841 GGGATGTCGG CGGGGTGCTT CACGTAGCCT TGGAGCCGTA CATGAACTGA GGGGGACAGG   901 ATGTCCCAGG CGAAGGGCAG GGGGCCCACC CTTGGTCACC CTTCAGCTTG GCGGTCTGGG   961 TGCCCTCGTA GGGCGGGCCC TCGCCCTCGC CCTCGATCTC GAAACTCGTG ACCGGTTTCA  1021 CGGGAGCCCC TCCCATGTGC ACTTTGGAAG CGGCATGAAC TCCGTTGATG AATGACAATG 1081 GTTAATTCAT CTTCGACCCG TGGCCTTCAG A //
Primer: PGKpro_F Sequence file: RDB16817_A8Lpd.seq
>D06157B4_A8LP_2_PGKpro_F_H06_24_ABI24.ab1     1 AAGGGGGGGC GTGTTCTCTC TTCTCATCTC CGGGCCTTTC GACCTGCATC CATCTAGATC    61 TCGAGCCACC ATGGCCAAGC CTTTGTCTCA AGAAGAATCC ACCCTCATTG AAAGAGCAAC   121 GGCTACAATC AACAGCATCC CCATCTCTGA AGACTACAGC GTCGCCAGCG CAGCTCTCTC   181 TAGCGACGGC CGCATCTTCA CTGGTGTCAA TGTATATCAT TTTACTGGGG GACCTTGTGC   241 AGAACTCGTG GTGCTGGGCA CTGCTGCTGC TGCGGCAGCT GGCAACCTGA CTTGTATCGT   301 CGCGATCGGA AATGAGAACA GGGGCATCTT GAGCCCCTGC GGACGGTGCC GACAGGTGCT   361 TCTCGATCTG CATCCTGGGA TCAAAGCCAT AGTGAAGGAC AGTGATGGAC AGCCGACGGC   421 AGTTGGGATT CGTGAATTGC TGCCCTCTGG TTATGTGTGG GAGGGCTAAT CGATGATATC   481 AGATCCCCGG GATGCAGAAA TTGATGATCT ATTAAACAAT AAAGATGTCC ACTAAAATGG   541 AAGTTTTTCC TGTCATACTT TGTTAAGAAG GGTGAGAACA GAGTACCTAC ATTTTGAATG   601 GAAGGATTGG AGCTACGGGG GTGGGGGTGG GGTGGGATTA GATAAATGCC TGCTCTTTAC   661 TGAAGGCTCT TTACTATTGC TTTATGATAA TGTTTCATAG TTGGATATCA TAATTTAAAC   721 AAGCAAAACC AAATTAAGGG CCAGCTCATT CCTCCCACTC ATGATCTATA GATCTATAGA   781 TCTCTCGTGG GATCATTGTT TTTCTCTTGA TTCCCACTTT GTGGTTCTAA GTACTGTGGT   841 TTCCAAATGT GTCAGTTTCA TAGCCTGAAG AACGAAGATC AGCAGCCTCT GTTCCACATA   901 CAACTTCATT CTCAGTATTG TTTTGCCAAG TTCTAATTTC CATCAGAAGC TGGGTCGAGA   961 TCCGGAACCC TTAATATAAA CTTCGTTATA TGTATGCTAT ACGAAGGTTA ATTAAGGTCC  1021 CTCGAAGAGG GTTCCACTTA GGAATCCGGG TACTCAGCCT TTTTGATTCC CTTTAGTGAG 1081 GGATTACTTT GCGCGCTGAC GGTCATCCAT GGGTCAATAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2021.03.11

GNP_filter3_RDBDEP_html_210308.pl