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pMK389 (mCherry2-BSD)

A plasmid for construction of C-terminal mCherry2-BSD tagging donors.

Catalog number RDB16817
Resource name pMK389 (mCherry2-BSD)
Clone info. A plasmid for construction of C-terminal mCherry2-BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Discosoma sp. RFP cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Discosoma sp. RFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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            Remarks
            The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose).
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            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB16817 pMK389 (mCherry2-BSD) DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Auxin Inducible Degron (AID) System (English text)
            Featured content Auxin Inducible Degron (AID) System (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB16817_A8Lpp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pUC-M13_-100
            Sequence file: RDB16817_A8Lpa.seq check
            >D06157B4_A8LP_2_pUC-M13_-100_E06_15_ABI24.ab1
                1 GAAGGTCAAG CGATTAGGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
               61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CGGATCCGGT
              121 GCAGGCGCCG CTAGCATGGT GAGCAAGGGC GAGGAGGATA ACATGGCCAT CATCAAGGAG
              181 TTCATGCGCT TCAAGGTGCA CATGGAGGGC TCCGTGAACG GCCACGAGTT CGAGATCGAG
              241 GGCGAGGGCG AGGGCCGCCC CTACGAGGGC ACCCAGACCG CCAAGCTGAA GGTGACCAAG
              301 GGTGGCCCCC TGCCCTTCGC CTGGGACATC CTGTCCCCTC AGTTCATGTA CGGCTCCAAG
              361 GCCTACGTGA AGCACCCCGC CGACATCCCC GACTACTTGA AGCTGTCCTT CCCCGAGGGC
              421 TTCAATTGGG AGCGCGTGAT GAACTTCGAG GACGGCGGCG TGGTGACCGT GACCCAGGAC
              481 TCCTCCCTGC AGGACGGCGA GTTCATCTAC AAGGTGAAGC TGCGCGGCAC CAACTTCCCC
              541 TCCGACGGCC CCGTAATGCA GTGTCGTACC ATGGGCTGGG AGGCCTCCAC TGAGCGGATG
              601 TACCCCGAGG ACGGCGCCCT GAAGGGCGAG ATCAAGCAGA GGCTGAAGCT GAAGGACGGC
              661 GGCCACTACG ACGCTGAGGT CAAGACCACC TACAAGGCCA AGAAGCCCGT GCAGCTGCCC
              721 GGCGCCTACA ACGTCGACAT CAAGTTGGAC ATCCTTTCCC ACAACGAGGA CTACACCATC
              781 GTGGAACAGT ACGAACGCGC CGAGGGCCGC CACTCCACCG GCGGCATGGA CGAGCTGTAC
              841 AAGTAATCTA GATAACTGAT CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT
              901 TTAAAAAAAC CTCCCACACC TCCCCCCTGA AACCTGAAAC ATAAAAATGA AATGCAATTT
              961 GTTGTTTGTT TAACTTTGTT TATTTGCAGC TTTATAAATG ATTTACAAAT AAAGCAAATT
             1021 AGCATCACAA TTTCACAAAT AAGCCAATTT TTTTTTCACT GCATTTCTAG TTGTGCTTGT
            1081 CCAAAACCTC ATCCAACTGT AATTCCGTA
            //
            Primer: reverse
            Sequence file: RDB16817_A8Lpb.seq check
            >D06157B4_A8LP_2_reverse_F06_18_ABI24.ab1
                1 CTAGCAAATT ACCCCTCACT AAAGGGAACA AAAGCTGGGT ACCGGATCCT AGTGAACCTC
               61 TTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATATT AAGGGTTCCG
              121 GATCTCGACC AGCTTCTGAT GGAATTAGAA CTTGGCAAAA CAATACTGAG AATGAAGTGT
              181 ATGTGGAACA GAGGCTGCTG ATCTCGTTCT TCAGGCTATG AAACTGACAC ATTTGGAAAC
              241 CACAGTACTT AGAACCACAA AGTGGGAATC AAGAGAAAAA CAATGATCCC ACGAGAGATC
              301 TATAGATCTA TAGATCATGA GTGGGAGGAA TGAGCTGGCC CTTAATTTGG TTTTGCTTGT
              361 TTAAATTATG ATATCCAACT ATGAAACATT ATCATAAAGC AATAGTAAAG AGCCTTCAGT
              421 AAAGAGCAGG CATTTATCTA ATCCCACCCC ACCCCCACCC CCGTAGCTCC AATCCTTCCA
              481 TTCAAAATGT AGGTACTCTG TTCTCACCCT TCTTAACAAA GTATGACAGG AAAAACTTCC
              541 ATTTTAGTGG ACATCTTTAT TGTTTAATAG ATCATCAATT TCTGCATCCC GGGGATCTGA
              601 TATCATCGAT TAGCCCTCCC ACACATAACC AGAGGGCAGC AATTCACGAA TCCCAACTGC
              661 CGTCGGCTGT CCATCACTGT CCTTCACTAT GGCTTTGATC CCAGGATGCA GATCGAGAAG
              721 CACCTGTCGG CACCGTCCGC AGGGGCTCAA GATGCCCCTG TTCTCATTTC CGATCGCGAC
              781 GATACAAGTC AGGTTGCCAG CTGCCGCAGC AGCAGCAGTG CCCAGCACCA CGAGTTCTGC
              841 ACAAGGTCCC CCCAGTAAAA TGATATACAT TGACACCAGT TGAAGATGCG GCCGTCGCTA
              901 GAGAGAGCTG CGCTGGCGAC GCTGTAGTCT TCAGAGATGG GGATGCTGTT GATTGTAGCC
              961 GTTGCTCTTT CAATGAGGGT GGATTTCTTC TTGAGACAAA GGCTTGCCCA TTGATGGCTC
             1021 GGAGATCTAG AATGAATGCC AAGGTTCGAA CGGCCGAGAG ATTGCAGAAG AAGGAAGTAA
            1081 CAGCGCCGGT CAAGACCGAG
            //
            Primer: PGK_N_rev
            Sequence file: RDB16817_A8Lpc.seq check
            >D06157B4_A8LP_2_PGK_N_rev_G06_21_ABI24.ab1
                1 CGTGGGGCGG CTCCCCTACC CGGTAGATTT CGAGGTCGAG ATCCTAAGCT TGGCTGGACG
               61 TAAACTCCTC TTCAGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG
              121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA
              181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA
              241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG
              301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
              361 CTGATTATGA TCAGTTATCT AGATTACTTG TACAGCTCGT CCATGCCGCC GGTGGAGTGG
              421 CGGCCCTCGG CGCGTTCGTA CTGTTCCACG ATGGTGTAGT CCTCGTTGTG GGAAAGGATG
              481 TCCAACTTGA TGTCGACGTT GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT GGCCTTGTAG
              541 GTGGTCTTGA CCTCAGCGTC GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT CTGCTTGATC
              601 TCGCCCTTCA GGGCGCCGTC CTCGGGGTAC ATCCGCTCAG TGGAGGCCTC CCAGCCCATG
              661 GTACGACACT GCATTACGGG GCCGTCGGAG GGGAAGTTGG TGCCGCGCAG CTTCACCTTG
              721 TAGATGAACT CGCCGTCCTG CAGGGAGGAG TCCTGGGTCA CGGTCACCAC GCCGCCGTCC
              781 TCGAAGTTCA TCACGCGCTC CCAATTGAAG CCCTCGGGGA AGGACAGCTT CAAGTAGTCG
              841 GGGATGTCGG CGGGGTGCTT CACGTAGCCT TGGAGCCGTA CATGAACTGA GGGGGACAGG
              901 ATGTCCCAGG CGAAGGGCAG GGGGCCCACC CTTGGTCACC CTTCAGCTTG GCGGTCTGGG
              961 TGCCCTCGTA GGGCGGGCCC TCGCCCTCGC CCTCGATCTC GAAACTCGTG ACCGGTTTCA
             1021 CGGGAGCCCC TCCCATGTGC ACTTTGGAAG CGGCATGAAC TCCGTTGATG AATGACAATG
            1081 GTTAATTCAT CTTCGACCCG TGGCCTTCAG A
            //
            Primer: PGKpro_F
            Sequence file: RDB16817_A8Lpd.seq check
            >D06157B4_A8LP_2_PGKpro_F_H06_24_ABI24.ab1
                1 AAGGGGGGGC GTGTTCTCTC TTCTCATCTC CGGGCCTTTC GACCTGCATC CATCTAGATC
               61 TCGAGCCACC ATGGCCAAGC CTTTGTCTCA AGAAGAATCC ACCCTCATTG AAAGAGCAAC
              121 GGCTACAATC AACAGCATCC CCATCTCTGA AGACTACAGC GTCGCCAGCG CAGCTCTCTC
              181 TAGCGACGGC CGCATCTTCA CTGGTGTCAA TGTATATCAT TTTACTGGGG GACCTTGTGC
              241 AGAACTCGTG GTGCTGGGCA CTGCTGCTGC TGCGGCAGCT GGCAACCTGA CTTGTATCGT
              301 CGCGATCGGA AATGAGAACA GGGGCATCTT GAGCCCCTGC GGACGGTGCC GACAGGTGCT
              361 TCTCGATCTG CATCCTGGGA TCAAAGCCAT AGTGAAGGAC AGTGATGGAC AGCCGACGGC
              421 AGTTGGGATT CGTGAATTGC TGCCCTCTGG TTATGTGTGG GAGGGCTAAT CGATGATATC
              481 AGATCCCCGG GATGCAGAAA TTGATGATCT ATTAAACAAT AAAGATGTCC ACTAAAATGG
              541 AAGTTTTTCC TGTCATACTT TGTTAAGAAG GGTGAGAACA GAGTACCTAC ATTTTGAATG
              601 GAAGGATTGG AGCTACGGGG GTGGGGGTGG GGTGGGATTA GATAAATGCC TGCTCTTTAC
              661 TGAAGGCTCT TTACTATTGC TTTATGATAA TGTTTCATAG TTGGATATCA TAATTTAAAC
              721 AAGCAAAACC AAATTAAGGG CCAGCTCATT CCTCCCACTC ATGATCTATA GATCTATAGA
              781 TCTCTCGTGG GATCATTGTT TTTCTCTTGA TTCCCACTTT GTGGTTCTAA GTACTGTGGT
              841 TTCCAAATGT GTCAGTTTCA TAGCCTGAAG AACGAAGATC AGCAGCCTCT GTTCCACATA
              901 CAACTTCATT CTCAGTATTG TTTTGCCAAG TTCTAATTTC CATCAGAAGC TGGGTCGAGA
              961 TCCGGAACCC TTAATATAAA CTTCGTTATA TGTATGCTAT ACGAAGGTTA ATTAAGGTCC
             1021 CTCGAAGAGG GTTCCACTTA GGAATCCGGG TACTCAGCCT TTTTGATTCC CTTTAGTGAG
            1081 GGATTACTTT GCGCGCTGAC GGTCATCCAT GGGTCAATAG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

            2021.11.02

            GNP_filter3_RDBDEP_html_211029.pl