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pMK389 (mCherry2-BSD)

A plasmid for construction of C-terminal mCherry2-BSD tagging donors.

Catalog number RDB16817
Resource name pMK389 (mCherry2-BSD)
Clone info. A plasmid for construction of C-terminal mCherry2-BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Discosoma sp. RFP cDNA
Depositor Kanemaki, Masato |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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Remarks
The BIOLOGICAL RESOURCE may not be used for COMMERCIAL PURPOSES. Please read the Exclusive MTA (Clontech FPs).
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RDB16817 pMK389 (mCherry2-BSD) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16817_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 RDB16817_A8Lpa.seq
>D06157B4_A8LP_2_pUC-M13_-100_E06_15_ABI24.ab1
    1 GAAGGTCAAG CGATTAGGTT GGGTACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
   61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CGGATCCGGT
  121 GCAGGCGCCG CTAGCATGGT GAGCAAGGGC GAGGAGGATA ACATGGCCAT CATCAAGGAG
  181 TTCATGCGCT TCAAGGTGCA CATGGAGGGC TCCGTGAACG GCCACGAGTT CGAGATCGAG
  241 GGCGAGGGCG AGGGCCGCCC CTACGAGGGC ACCCAGACCG CCAAGCTGAA GGTGACCAAG
  301 GGTGGCCCCC TGCCCTTCGC CTGGGACATC CTGTCCCCTC AGTTCATGTA CGGCTCCAAG
  361 GCCTACGTGA AGCACCCCGC CGACATCCCC GACTACTTGA AGCTGTCCTT CCCCGAGGGC
  421 TTCAATTGGG AGCGCGTGAT GAACTTCGAG GACGGCGGCG TGGTGACCGT GACCCAGGAC
  481 TCCTCCCTGC AGGACGGCGA GTTCATCTAC AAGGTGAAGC TGCGCGGCAC CAACTTCCCC
  541 TCCGACGGCC CCGTAATGCA GTGTCGTACC ATGGGCTGGG AGGCCTCCAC TGAGCGGATG
  601 TACCCCGAGG ACGGCGCCCT GAAGGGCGAG ATCAAGCAGA GGCTGAAGCT GAAGGACGGC
  661 GGCCACTACG ACGCTGAGGT CAAGACCACC TACAAGGCCA AGAAGCCCGT GCAGCTGCCC
  721 GGCGCCTACA ACGTCGACAT CAAGTTGGAC ATCCTTTCCC ACAACGAGGA CTACACCATC
  781 GTGGAACAGT ACGAACGCGC CGAGGGCCGC CACTCCACCG GCGGCATGGA CGAGCTGTAC
  841 AAGTAATCTA GATAACTGAT CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT
  901 TTAAAAAAAC CTCCCACACC TCCCCCCTGA AACCTGAAAC ATAAAAATGA AATGCAATTT
  961 GTTGTTTGTT TAACTTTGTT TATTTGCAGC TTTATAAATG ATTTACAAAT AAAGCAAATT
 1021 AGCATCACAA TTTCACAAAT AAGCCAATTT TTTTTTCACT GCATTTCTAG TTGTGCTTGT
1081 CCAAAACCTC ATCCAACTGT AATTCCGTA
//
Primer: reverse RDB16817_A8Lpb.seq
>D06157B4_A8LP_2_reverse_F06_18_ABI24.ab1
    1 CTAGCAAATT ACCCCTCACT AAAGGGAACA AAAGCTGGGT ACCGGATCCT AGTGAACCTC
   61 TTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATATT AAGGGTTCCG
  121 GATCTCGACC AGCTTCTGAT GGAATTAGAA CTTGGCAAAA CAATACTGAG AATGAAGTGT
  181 ATGTGGAACA GAGGCTGCTG ATCTCGTTCT TCAGGCTATG AAACTGACAC ATTTGGAAAC
  241 CACAGTACTT AGAACCACAA AGTGGGAATC AAGAGAAAAA CAATGATCCC ACGAGAGATC
  301 TATAGATCTA TAGATCATGA GTGGGAGGAA TGAGCTGGCC CTTAATTTGG TTTTGCTTGT
  361 TTAAATTATG ATATCCAACT ATGAAACATT ATCATAAAGC AATAGTAAAG AGCCTTCAGT
  421 AAAGAGCAGG CATTTATCTA ATCCCACCCC ACCCCCACCC CCGTAGCTCC AATCCTTCCA
  481 TTCAAAATGT AGGTACTCTG TTCTCACCCT TCTTAACAAA GTATGACAGG AAAAACTTCC
  541 ATTTTAGTGG ACATCTTTAT TGTTTAATAG ATCATCAATT TCTGCATCCC GGGGATCTGA
  601 TATCATCGAT TAGCCCTCCC ACACATAACC AGAGGGCAGC AATTCACGAA TCCCAACTGC
  661 CGTCGGCTGT CCATCACTGT CCTTCACTAT GGCTTTGATC CCAGGATGCA GATCGAGAAG
  721 CACCTGTCGG CACCGTCCGC AGGGGCTCAA GATGCCCCTG TTCTCATTTC CGATCGCGAC
  781 GATACAAGTC AGGTTGCCAG CTGCCGCAGC AGCAGCAGTG CCCAGCACCA CGAGTTCTGC
  841 ACAAGGTCCC CCCAGTAAAA TGATATACAT TGACACCAGT TGAAGATGCG GCCGTCGCTA
  901 GAGAGAGCTG CGCTGGCGAC GCTGTAGTCT TCAGAGATGG GGATGCTGTT GATTGTAGCC
  961 GTTGCTCTTT CAATGAGGGT GGATTTCTTC TTGAGACAAA GGCTTGCCCA TTGATGGCTC
 1021 GGAGATCTAG AATGAATGCC AAGGTTCGAA CGGCCGAGAG ATTGCAGAAG AAGGAAGTAA
1081 CAGCGCCGGT CAAGACCGAG
//
Primer: PGK_N_rev RDB16817_A8Lpc.seq
>D06157B4_A8LP_2_PGK_N_rev_G06_21_ABI24.ab1
    1 CGTGGGGCGG CTCCCCTACC CGGTAGATTT CGAGGTCGAG ATCCTAAGCT TGGCTGGACG
   61 TAAACTCCTC TTCAGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG
  121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA
  181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA
  241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG
  301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
  361 CTGATTATGA TCAGTTATCT AGATTACTTG TACAGCTCGT CCATGCCGCC GGTGGAGTGG
  421 CGGCCCTCGG CGCGTTCGTA CTGTTCCACG ATGGTGTAGT CCTCGTTGTG GGAAAGGATG
  481 TCCAACTTGA TGTCGACGTT GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT GGCCTTGTAG
  541 GTGGTCTTGA CCTCAGCGTC GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT CTGCTTGATC
  601 TCGCCCTTCA GGGCGCCGTC CTCGGGGTAC ATCCGCTCAG TGGAGGCCTC CCAGCCCATG
  661 GTACGACACT GCATTACGGG GCCGTCGGAG GGGAAGTTGG TGCCGCGCAG CTTCACCTTG
  721 TAGATGAACT CGCCGTCCTG CAGGGAGGAG TCCTGGGTCA CGGTCACCAC GCCGCCGTCC
  781 TCGAAGTTCA TCACGCGCTC CCAATTGAAG CCCTCGGGGA AGGACAGCTT CAAGTAGTCG
  841 GGGATGTCGG CGGGGTGCTT CACGTAGCCT TGGAGCCGTA CATGAACTGA GGGGGACAGG
  901 ATGTCCCAGG CGAAGGGCAG GGGGCCCACC CTTGGTCACC CTTCAGCTTG GCGGTCTGGG
  961 TGCCCTCGTA GGGCGGGCCC TCGCCCTCGC CCTCGATCTC GAAACTCGTG ACCGGTTTCA
 1021 CGGGAGCCCC TCCCATGTGC ACTTTGGAAG CGGCATGAAC TCCGTTGATG AATGACAATG
1081 GTTAATTCAT CTTCGACCCG TGGCCTTCAG A
//
Primer: PGKpro_F RDB16817_A8Lpd.seq
>D06157B4_A8LP_2_PGKpro_F_H06_24_ABI24.ab1
    1 AAGGGGGGGC GTGTTCTCTC TTCTCATCTC CGGGCCTTTC GACCTGCATC CATCTAGATC
   61 TCGAGCCACC ATGGCCAAGC CTTTGTCTCA AGAAGAATCC ACCCTCATTG AAAGAGCAAC
  121 GGCTACAATC AACAGCATCC CCATCTCTGA AGACTACAGC GTCGCCAGCG CAGCTCTCTC
  181 TAGCGACGGC CGCATCTTCA CTGGTGTCAA TGTATATCAT TTTACTGGGG GACCTTGTGC
  241 AGAACTCGTG GTGCTGGGCA CTGCTGCTGC TGCGGCAGCT GGCAACCTGA CTTGTATCGT
  301 CGCGATCGGA AATGAGAACA GGGGCATCTT GAGCCCCTGC GGACGGTGCC GACAGGTGCT
  361 TCTCGATCTG CATCCTGGGA TCAAAGCCAT AGTGAAGGAC AGTGATGGAC AGCCGACGGC
  421 AGTTGGGATT CGTGAATTGC TGCCCTCTGG TTATGTGTGG GAGGGCTAAT CGATGATATC
  481 AGATCCCCGG GATGCAGAAA TTGATGATCT ATTAAACAAT AAAGATGTCC ACTAAAATGG
  541 AAGTTTTTCC TGTCATACTT TGTTAAGAAG GGTGAGAACA GAGTACCTAC ATTTTGAATG
  601 GAAGGATTGG AGCTACGGGG GTGGGGGTGG GGTGGGATTA GATAAATGCC TGCTCTTTAC
  661 TGAAGGCTCT TTACTATTGC TTTATGATAA TGTTTCATAG TTGGATATCA TAATTTAAAC
  721 AAGCAAAACC AAATTAAGGG CCAGCTCATT CCTCCCACTC ATGATCTATA GATCTATAGA
  781 TCTCTCGTGG GATCATTGTT TTTCTCTTGA TTCCCACTTT GTGGTTCTAA GTACTGTGGT
  841 TTCCAAATGT GTCAGTTTCA TAGCCTGAAG AACGAAGATC AGCAGCCTCT GTTCCACATA
  901 CAACTTCATT CTCAGTATTG TTTTGCCAAG TTCTAATTTC CATCAGAAGC TGGGTCGAGA
  961 TCCGGAACCC TTAATATAAA CTTCGTTATA TGTATGCTAT ACGAAGGTTA ATTAAGGTCC
 1021 CTCGAAGAGG GTTCCACTTA GGAATCCGGG TACTCAGCCT TTTTGATTCC CTTTAGTGAG
1081 GGATTACTTT GCGCGCTGAC GGTCATCCAT GGGTCAATAG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

2019.07.12

GNP_filter3_RDBDEP_html_190431.pl