Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK293 (mAID-mCherry2-Hygro)

A plasmid for construction of C-terminal mAID-mCherry tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Please contact us to request the Exclusive MTA.
Remarks	This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。
備考	Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13936_A6B8p1-2

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410) Region: Linker, mAID, mCherry2 5' Sequence file: RDB13936_A6B8e.seq
>D03070A20_A6B8_1_M13(-40)_E05_14.ab1     1 CGCGGGACGC GCGTATACGA CTCACTATAG GGCGAATTGG AGCTCCCCGG GATCCGGTGC    61 AGGCGCCAAG GAGAAGAGTG CTTGTCCTAA AGATCCAGCC AAACCTCCGG CCAAGGCACA   121 AGTTGTGGGA TGGCCACCGG TGAGATCATA CCGGAAGAAC GTGATGGTTT CCTGCCAAAA   181 ATCAAGCGGT GGCCCGGAGG CGGCGGCGTT CGTGAAGGTA TCAATGGACG GAGCACCGTA   241 CTTGAGGAAA ATCGATTTGA GGATGTATAA AGCTAGCATG GTGAGCAAGG GCGAGGAGGA   301 TAACATGGCC ATCATCAAGG AGTTCATGCG CTTCAAGGTG CACATGGAGG GCTCCGTGAA   361 CGGCCACGAG TTCGAGATCG AGGGCGAGGG CGAGGGCCGC CCCTACGAGG GCACCCAGAC   421 CGCCAAGCTG AAGGTGACCA AGGGTGGCCC CCTGCCCTTC GCCTGGGACA TCCTGTCCCC   481 TCAGTTCATG TACGGCTCCA AGGCCTACGT GAAGCACCCC GCCGACATCC CCGACTACTT   541 GAAGCTGTCC TTCCCCGAGG GCTTCAATTG GGAGCGCGTG ATGAACTTCG AGGACGGCGG   601 CGTGGTGACC GTGACCCAGG ACTCCTCCCT GCAGGACGGC GAGTTCATCT ACAAGGTGAA   661 GCTGCGCGGC ACCAACTTCC CCTCCGACGG CCCCGTAATG CAGTGTCGTA CCATGGGCTG   721 GGAGGCCTCC ACTGAGCGGA TGTACCCCGA GGACGGCGCC CTGAAGGGCG AGATCAAGCA   781 GAGGCTGAAG CTGAAGGACG GCGGCCACTA CGACGCTGAG GTCAAGACCA CCTACAAGGC   841 CAAGAAGCCC GTGCAGCTGC CCGGCGCCTA CAACGTCGAC ATCAAGTTGG ACATCCTTTT   901 CCCACAACGA GGACTACACC ATCGTGGAAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC   961 CACCGGGCGG CATGGACGAG CTGTACAAGT AATCTAGATA ACTGATCATA ATCAGCCATA  1021 CCACATTTGT AGAGGGTTTT TACTTTGCTT TAAAAAACTC CCACACCTCC CCCTGACCTG  1081 AAACATAAAA TGATGCAATT GAGTGTACCT GGTTAATTGC AGCCTAATAA TGGATTACAA 1141 AATTAAAGGC AATTAG //
Primer: PGK_N_rev (Pr0243) Region: loxP, SV40 p(A), mCherry2 3' Sequence file: RDB13936_A6B8f.seq
>D03070A20_A6B8_1_PGK-N-rev_G06_21.ab1     1 CATGGACGCT CCCTACCGGT AGATTTCGAG GTCGAGATCC TAAGCTTGGC TGGACGTAAA    61 CTCCTCTTCA GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT   121 ATTGAATATG ATCGACGCGT TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT   181 GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT   241 TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA   301 GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA   361 TTATGATCAG TTATCTAGAT TACTTGTACA GCTCGTCCAT GCCGCCGGTG GAGTGGCGGC   421 CCTCGGCGCG TTCGTACTGT TCCACGATGG TGTAGTCCTC GTTGTGGGAA AGGATGTCCA   481 ACTTGATGTC GACGTTGTAG GCGCCGGGCA GCTGCACGGG CTTCTTGGCC TTGTAGGTGG   541 TCTTGACCTC AGCGTCGTAG TGGCCGCCGT CCTTCAGCTT CAGCCTCTGC TTGATCTCGC   601 CCTTCAGGGC GCCGTCCTCG GGGTACATCC GCTCAGTGGA GGCCTCCCAG CCCATGGTAC   661 GACACTGCAT TACGGGGCCG TCGGAGGGGA AGTTGGTGCC GCGCAGCTTC ACCTTGTAGA   721 TGAACTCGCC GTCCTGCAGG GAGGAGTCCT GGGTCACGGT CACCACGCCG CCGTCCTCGA   781 AGTTCATCAC GCGCTCCCAA TTGAAGCCCT CGGGGAAGGA CAGCTTCAAG TAGTCGGGGA   841 TGTCGGCGGG GTGCTTCACG TAGGCCTTGG AGCCGTACAT GAACTGAAGG GGACAGGATG   901 TCCCAGGCGA AGGCAGGGGG CCCACCTTGG GTCACCTTCA GCTTGGCGGT CTGGGTGCCC   961 TCGTAAGGGC GGCCCTCGCC CCTCGCCCTC GAATCTCGAA CTCGTGGCCC GTTCACGGAG  1021 CCCTCATGTG CACCTTGAAG CCGCATGGAA CCTCCCTGGA TGATGTGACC ATGTTATCCA  1081 TCATCAGCCT TGCTTCACCC ATGGCTAGCC CTTATAGCAT CTCGAATTCG AATTCTTCAA 1141 GTACGGTCTC GTTCCATTGG ATACCATCAA CT //
Primer: PGKpro_F (Pr0249) Region: PGK pro, HygR 5' Sequence file: RDB13936_A6B8g.seq
>D03070A20_A6B8_1_PGK-proF_B08_05.ab1     1 ACCCGCGATC TCTCTTCTCA TCTCCGGGCC TTTCGACCTG CATCCATCTA GATCTCGAGC    61 AGCTGAAGCT TACCGCTAGC ATGGATAGAT CCGGAAAGCC TGAACTCACC GCGACGTCTG   121 TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA CCTGATGCAG CTCTCGGAGG   181 GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG TGGATATGTC CTGCGGGTAA   241 ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA TCGGCACTTT GCATCGGCCG   301 CGCTCCCGAT TCCGGAAGTG CTTGACATTG GGGAATTCAG CGAGAGCCTG ACCTATTGCA   361 TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA CTGCCCGCTG   421 TTCTGCAGCC GGTCGCGGAG GCCATGGATG CGATCGCTGC GGCCGATCTT AGCCAGACGA   481 GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA CACTACATGG CGTGATTTCA   541 TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC TGTGATGGAC GACACCGTCA   601 GTGCGTCCGT CGCGCAGGCT CTCGATGAGC TGATGCTTTG GGCCGAGGAC TGCCCCGAAG   661 TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGAC AATGGCCGCA   721 TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGA TTCCCAATAC GAGGTCGCCA   781 ACATCTTCTT CTGGAGGCCG TGGTTGGCTT GTATGGAGCA GCAGACGCGC TACTTCGAGC   841 GGAGGCATCC GGAGCTTGCA GATCGCCGCG GCTCCGGGCG TATATGCTCC GCATTGGTCT   901 TGACCAACTC TATCAGAGCT TGGTTGACGG CAATTTCGAT GATGCAGCTT GGGCGCAGGG   961 TCGATGCGAC GCAATCGTCC CGATCCGGAG CCGGGACTGT CGGGGCGTAC ACAAATCGCC  1021 GCAGAAGCGC GGCCGTCTGG ACGATGGCTG TGTAGAGTAC TTCGCGATAG TGGAACGACG  1081 CTCAGCACTC GTCCGAGCAA AGATTAGCTA GCCGGCCGTC CCCCACGACC CCGCCCAGTC 1141 G //
Primer: M13R (Pr0160) Region: loxP, mouse PGK ter, HygR 3' Sequence file: RDB13936_A6B8h.seq
>D03070A20_A6B8_1_M13R_E04_13.ab1     1 CTCGCGCATT AACCTCACTA AAGGGACAAA AGCTGGGTAC CGGATCCTAG TGAACCTCTT    61 CGAGGGACCT AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA GGGTTCCGGA   121 TCTCGACCAG CTTCTGATGG AATTAGAACT TGGCAAAACA ATACTGAGAA TGAAGTGTAT   181 GTGGAACAGA GGCTGCTGAT CTCGTTCTTC AGGCTATGAA ACTGACACAT TTGGAAACCA   241 CAGTACTTAG AACCACAAAG TGGGAATCAA GAGAAAAACA ATGATCCCAC GAGAGATCTA   301 TAGATCTATA GATCATGAGT GGGAGGAATG AGCTGGCCCT TAATTTGGTT TTGCTTGTTT   361 AAATTATGAT ATCCAACTAT GAAACATTAT CATAAAGCAA TAGTAAAGAG CCTTCAGTAA   421 AGAGCAGGCA TTTATCTAAT CCCACCCCAC CCCCACCCCC GTAGCTCCAA TCCTTCCATT   481 CAAAATGTAG GTACTCTGTT CTCACCCTTC TTAACAAAGT ATGACAGGAA AAACTTCCAT   541 TTTAGTGGAC ATCTTTATTG TTTAATAGAT CATCAATTTC TGCATCCCGG GGATCTGATA   601 TCATCGATGC ATGGGGTCGT GCGCTCCTTT CGGTCGGGCG CTGCGGGTCG TGGGGCGGGC   661 GGCTAGCCTA TTCCTTTGCC CTCGGACGAG TGCTGGGGCG TCGGTTTCCA CTATCGGCGA   721 GTACTTCTAC ACAGCCATCG GTCCAGACGG CCGCGCTTCT GCGGGCGATT TGTGTACGCC   781 CGACAGTCCC GGCTCCGGAT CGGACGATTG CGTCGCATCG ACCCTGCGCC CAAGCTGCAT   841 CATCGAAATT GCCGTCAACC AAGCTCTGAT AGAGTTGGTC AAGACCAATG CGGAGCATAT   901 ACGCCCGGAG CCGCGGCGAT CCTGCAAGCT CCGGATGCCT CCGCTCGAAG TAGCGCGTCT   961 GCTGCTCCAT ACAAGCCCAA CCCACGGGCC TCCAGAAGAA GATGTTGGCG ACCTCGTATT  1021 GGGGAATCCC CGAACATCGC TCGCTCAGTC ATGAACCGCT GTATGCCGGC ATTGTCGTCA 1081 GGGACATGAT GAGCGGAATT CGCGTGCACG AGTGCCGGAA CCTTCCGGGG GGATCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.02.27

GNP_filter3_RDBDEP_html_230227.pl