Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK293 (mAID-mCherry2-Hygro)

A plasmid for construction of C-terminal mAID-mCherry tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	提供依頼書 [Word] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13936_A6B8p1-4.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410) Region: T7 pro,insert 5'(Linker,mAID,mCherry2 5') Sequence file: RDB13936_A6B8i.seq
>D03070A20_A6B8_1_M13(-40)_E05_14.ab1     1 NNNNNNNNNC NCNNANNNGN NTNNCNATAG GGCGAATTGG AGCTCCCCGG GNTCCGGTGC    61 AGGCGCCAAG GAGAAGAGTG CTTGTCCTAA AGATCCAGCC AAACCTCCGG CCAAGGCACA   121 AGTTGTGGGA TGGCCACCGG TGAGATCATA CCGGAAGAAC GTGATGGTTT CCTGCCAAAA   181 ATCAAGCGGT GGCCCGGAGG CGGCGGCGTT CGTGAAGGTA TCAATGGACG GAGCACCGTA   241 CTTGAGGAAA ATCGATTTGA GGATGTATAA AGCTAGCATG GTGAGCAAGG GCGAGGAGGA   301 TAACATGGCC ATCATCAAGG AGTTCATGCG CTTCAAGGTG CACATGGAGG GCTCCGTGAA   361 CGGCCACGAG TTCGAGATCG AGGGCGAGGG CGAGGGCCGC CCCTACGAGG GCACCCAGAC   421 CGCCAAGCTG AAGGTGACCA AGGGTGGCCC CCTGCCCTTC GCCTGGGACA TCCTGTCCCC   481 TCAGTTCATG TACGGCTCCA AGGCCTACGT GAAGCACCCC GCCGACATCC CCGACTACTT   541 GAAGCTGTCC TTCCCCGAGG GCTTCAATTG GGAGCGCGTG ATGAACTTCG AGGACGGCGG   601 CGTGGTGACC GTGACCCAGG ACTCCTCCCT GCAGGACGGC GAGTTCATCT ACAAGGTGAA   661 GCTGCGCGGC ACCAACTTCC CCTCCGACGG CCCCGTAATG CAGTGTCGTA CCATGGGCTG   721 GGANGCCTCC ACTGAGCGGA TGTACCCCGA GGACGGCGCC CTGAAGGGCG AGATCAAGCA   781 GANGCTGAAG CTGAANGACG GCGGCCACTA CGACGCTGAN GTCAAGACCA CCTACAAGGC   841 CNAGAAGCCC GTGCAGCTGC CCGGCGCCTA CNACGTCNAC ATCAAGTTGG ANATCCTTTT   901 NCCACNACGA NGANNACNNN NTCNNGGNAN CAGTACNAAC GCNCCNAGGG NCGCCNNNNC   961 NNNNNGNNGG CATGGNNNAN CTGTNNNANT NATCNANNNN NCTGNTCNNA ANCANCCNTA 1021 NNANNNTNNN NNNNGNNTTT NNNNNNNNNT NANAAANNNN NNNNNN //
Primer: M13R (Pr0160) Region: insert 3'(loxP,mouse PGK ter,HygR) Sequence file: RDB13936_A6B8j.seq
>D03070A20_A6B8_1_M13R_E04_13.ab1     1 NNNNNNNNNN NNNNNNNNNN ANGNNNNNNA AGCTGGGTAC CGGATCCTAG TGAACCTCTT    61 CGAGGGACCT AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA GGGTTCCGGA   121 TCTCGACCAG CTTCTGATGG AATTAGAACT TGGCAAAACA ATACTGAGAA TGAAGTGTAT   181 GTGGAACAGA GGCTGCTGAT CTCGTTCTTC AGGCTATGAA ACTGACACAT TTGGAAACCA   241 CAGTACTTAG AACCACAAAG TGGGAATCAA GAGAAAAACA ATGATCCCAC GAGAGATCTA   301 TAGATCTATA GATCATGAGT GGGAGGAATG AGCTGGCCCT TAATTTGGTT TTGCTTGTTT   361 AAATTATGAT ATCCAACTAT GAAACATTAT CATAAAGCAA TAGTAAAGAG CCTTCAGTAA   421 AGAGCAGGCA TTTATCTAAT CCCACCCCAC CCCCACCCCC GTAGCTCCAA TCCTTCCATT   481 CAAAATGTAG GTACTCTGTT CTCACCCTTC TTAACAAAGT ATGACAGGAA AAACTTCCAT   541 TTTAGTGGAC ATCTTTATTG TTTAATAGAT CATCAATTTC TGCATCCCGG GGATCTGATA   601 TCATCGATGC ATGGGGTCGT GCGCTCCTTT CGGTCGGGCG CTGCGGGTCG TGGGGCGGGC   661 GGCTAGCCTA TTCCTTTGCC CTCGGACGAG TGCTGGGGCG TCGGTTTCCA CTATCGGCGA   721 GTACTTCTAC ACAGCCATCG GTCCAGACGG CCGCGCTTCT GCGGGCGANT TGTGTACGCC   781 CGACAGTCCC GGCTCCGGAT CGGACGATTG CGTCGCATCN ACCCNGCGCC CAAGCTGCAT   841 CATCNAAATT GCCGTCNACC AAGCTCTGAT AGAGTTGGTC AAGANNAATG CNNANCATAT   901 ACGCCNNGAN CCNCGGCGAT NCNGCAANCT CCNGANGNCN NNNCNCNNNN TANCNNGNCN 961 GCNGNNNNNT ANNNNNCNAN NCNNNNGNNN NNNNNNN //
Primer: PGK_N_rev (Pr0243) Region: insert mid(loxP,SV40 pA,mCherry2 3') Sequence file: RDB13936_A6B8k.seq
>D03070A20_A6B8_1_PGK-N-rev_G06_21.ab1     1 NNNNNNNNNN NNNNNNNNGN ANANTTCGAG GTCGAGNTCC TAAGCTTGGC TGGACGTAAA    61 CTCCTCTTCA GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT   121 ATTGAATATG ATCGACGCGT TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT   181 GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT   241 TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA   301 GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA   361 TTATGATCAG TTATCTAGAT TACTTGTACA GCTCGTCCAT GCCGCCGGTG GAGTGGCGGC   421 CCTCGGCGCG TTCGTACTGT TCCACGATGG TGTAGTCCTC GTTGTGGGAA AGGATGTCCA   481 ACTTGATGTC GACGTTGTAG GCGCCGGGCA GCTGCACGGG CTTCTTGGCC TTGTAGGTGG   541 TCTTGACCTC AGCGTCGTAG TGGCCGCCGT CCTTCAGCTT CAGCCTCTGC TTGATCTCGC   601 CCTTCAGGGC GCCGTCCTCG GGGTACATCC GCTCAGTGGA GGCCTCCCAG CCCATGGTAC   661 GACACTGCAT TACGGGGCCG TCGGANGGGA AGTTGGTGCN NNNCAGCTTC ACCTTGTAGA   721 TGAACTCGCC GTCCTGCANG GAGGAGTCCT GGGTCACGGT CACCACGCCG CCNTCCTCGA   781 AGTTCATNAN NNNCTCCCNA TTGAAGCCCT CGGGGANNGN NAGCTTCNAG TAGTCNNGGA   841 NGTNNGCGGG NNGCTTCACG TNNNCCNNNN ANNNNNACAT GANNNNNNGG GGNNAGGNNG   901 NNCCANGNNN NNGNNNGNGG NCNNNCNTGG NNNNNCTTCN NCNNGNCNGT CNGGGTGNCN 961 NNNNNNNGGC NGNCCNNNNN NNNNN //
Primer: PGKpro_F (Pr0249) Region: insert mid( HygR 5') Sequence file: RDB13936_A6B8l.seq
>D03070A20_A6B8_1_PGK-proF_B08_05.ab1     1 NNNNNNNNNN NNNNNNNNNN TCTCCNGGCC TTTCGACCTG CATCCNTCTA GATCTCGAGC    61 AGCTGAAGCT TACCGCTAGC ATGGATAGAT CCGGAAAGCC TGAACTCACC GCGACGTCTG   121 TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA CCTGATGCAG CTCTCGGAGG   181 GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG TGGATATGTC CTGCGGGTAA   241 ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA TCGGCACTTT GCATCGGCCG   301 CGCTCCCGAT TCCGGAAGTG CTTGACATTG GGGAATTCAG CGAGAGCCTG ACCTATTGCA   361 TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA CTGCCCGCTG   421 TTCTGCAGCC GGTCGCGGAG GCCATGGATG CGATCGCTGC GGCCGATCTT AGCCAGACGA   481 GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA CACTACATGG CGTGATTTCA   541 TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC TGTGATGGAC GACACCGTCA   601 GTGCGTCCGT CGCGCAGGCT CTCGATGAGC TGATGCTTTG GGCCGAGGAC TGCCCCGAAG   661 TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGAC AATGGCCGCA   721 TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGN NTCCCAATAC GANGTCGCCA   781 ACATCTTCTT CTGGANGCCG TGGTTGGCTT GTATGGAGCA GCAGACGCGC TACTTCNAGC   841 GGANGCATCC NGANCTTGCN NNTCGCCGCG GCTCCNGGCG TATATGCTNC GCATTNGTCT   901 TGANNNACTC TATCNNANCT TGNTNGANNN NNNTTTCGAT GANGCNNCNN GGGNNNNNGN 961 NCNANGCNNC NNNNNNNNNC NNNNNNNNAN NNNGNNNNNN NNGGNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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