Please review the QC test results indicated by icon below as well as clone information before placing your order.

BRD4-N CRISPR

Expression vector of Cas9 and gRNA for a BRD4 tagging donor.

Sequence (full)	RDB18359zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Remarks	The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
備考	本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18359_B0Gfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3element Reverse (Pr0108) Region: U6 pro,sgRNA for BRD4-N,CBh pro Sequence file: RDB18359_B0Gfa.seq
>D07105C9_B0Gf_3_SV40pA3'element Reverse_A05_02_ABI24.ab1     1 NNNNNNNNNN NNNNNGNNNN NANGGNNNNN NCNNGCAACG CGGCCTTTTT ACGGTTCCTG    61 GCCTTTTNNN NNNNNNTTGC TCACATGTGA GGGCCTATTT CCCATGATTC CNTCNTATTT   121 GCATATACGA TACAAGGCTG TTAGAGAGAT AATTGGAATT AATTTGACTG TAAACACAAA   181 GATATTAGTA CAAAATACGT GACGTAGAAA GTAATAATTT CTTGGGTAGT TTGCAGTTTT   241 AAAATTATGT TTTAAAATGG ACTATCATAT GCTTACCGTA ACTTGAAAGT ATTTCGATTT   301 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAACACCGAT GTCTGCGGAG AGCGGCCCGT   361 TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG TTATCAACTT GAAAAAGTGG   421 CACCGAGTCG GTGCTTTTTT GTTTTAGAGC TAGAAATAGC AAGTTAAAAT AAGGCTAGTC   481 CGTTTTTAGC GCGTGCGCCA ATTCTGCAGA CAAATGGCTC TAGAGGTACC CGTTACATAA   541 CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA   601 GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC   661 CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC   721 GGTAAATGGC CCGCCTGGCA TTGTGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG   781 CAGTACATCT ACGTATTANT CATCGCTATT ACCATGGTCG ANGTGANCCC CACGTTCTGC   841 TTCACTCTCC CCATCTCCCC CCCCNNNCCN ACCCCCAATT TTGTANTTAT TTATTTTTTN 901 NATTATTTTG TGCANCGATG GGGGCGNNNN NNNNNN //
Primer: bGH_rev3 (Pr0885) Region: nucleoplasmin NLS,Cas9 Sequence file: RDB18359_B0Gfb.seq
>D07105C9_B0Gf_3_bGH_rev3_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGGGNNGG NNNNNNACNG ANGGCTGGCA ACTAGAAGGC    61 ACAGTCGAGG CTGATCAGCN AGCTCTAGGA ATTCTTACTT TTTCTTTTTT GCCTGGCCGG   121 CCTTTTTCGT GGCCGCCGGC CTTTTGTCGC CTCCCAGCTG AGACAGGTCG ATCCGTGTCT   181 CGTACAGGCC GGTGATGCTC TGGTGGATCA GGGTGGCGTC CAGCACCTCT TTGGTGCTGG   241 TGTACCTCTT CCGGTCGATG GTGGTGTCAA AGTACTTGAA GGCGGCAGGG GCTCCCAGAT   301 TGGTCAGGGT AAACAGGTGG ATGATATTCT CGGCCTGCTC TCTGATGGGC TTATCCCGGT   361 GCTTGTTGTA GGCGGACAGC ACTTTGTCCA GATTAGCGTC GGCCAGGATC ACTCTCTTGG   421 AGAACTCGCT GATCTGCTCG ATGATCTCGT CCAGGTAGTG CTTGTGCTGT TCCACAAACA   481 GCTGTTTCTG CTCATTATCC TCGGGGGAGC CCTTCAGCTT CTCATAGTGG CTGGCCAGGT   541 ACAGGAAGTT CACATATTTG GAGGGCAGGG CCAGTTCGTT TCCCTTCTGC AGTTCGCCGG   601 CAGAGGCCAG CATTCTCTTC CGGCCGTTTT CCAGCTCGAA CAGGGAGTAC TTAGGCAGCT   661 TGATGATCAG GTCCTTTTTC ACTTCTTTGT AGCCCTTGGC TTCCAGAAAG TCGATGGGAT   721 TCTTCTCGAA NCTGCTTCTT TCCATGATGG TGATCCCCAG CAGCTCTTTC ACACTCTTCA   781 NTTTCTTGGA CTTGCCCTTT TCCACTTTGG NCNCCACNNN NNNNNAATAN GCCACGGTGG   841 GGCTGTCNNN NNNNCNNNAC TTCTTAGGGT CCCAGTCCTT CTTTCTGGCG ATNANCTTAN   901 CNCTGNTCCT CNTGNNNNNG ANANANTCTT TNCTNNANNC CNCNTGNCNG NNNNTCNGNN   961 CNTTTTCNCN ATATTCACNN NGGGNNNNGC TNNANNNNNT TCCNCNNGGN GGNCNAANNC  1021 CCGGNCCNTN ATCCCNCNNN NATNNNCCNN NTTNCNNNGT NNGNCNNGAT NNNANNNNNN 1081 NNCNNNNNNN NNN //
Primer: Cas9-N_R1 (Pr0650) Region: CBh pro,3xFLAG, SV40 NLS,Cas9 Sequence file: RDB18359_B0Gfc.seq
>D07105C9_B0Gf_3_Cas9-N_R3_H01_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNGNCGA NGTCNNGGCC GANGCTGTAC TTCTTGTCGG    61 CTGCTGGGAC TCCGTGGATA CCGACCTTCC GCTTCTTCTT TGGGGCCATC TTATCGTCAT   121 CGTCTTTGTA ATCAATATCA TGATCCTTGT AGTCTCCGTC GTGGTCCTTA TAGTCCATGG   181 TGGCACCGGT CCAACCTGAA AAAAAGTGAT TTCAGGCAGG TGCTCCAGGT AATTAAACAT   241 TAATACCCCA CCAACCAACC ATCCCTTAAA CCCTTACCTC TTGCTCAGCT AATTACAGCC   301 CGGAGGAGAA GGGCCGTCCC GCCCGCTCAC CTGTGGGAGT AACGCGGTCA GTCAGAGCCG 361 GGGCGGGCGG CGCGAGG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl