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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

BRD4-N CRISPR

Expression vector of Cas9 and gRNA for a BRD4 tagging donor.

Catalog number RDB18359
Resource name BRD4-N CRISPR
Clone info. Expression vector of Cas9 and gRNA for a BRD4 tagging donor, see Fig. 5 of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone px330 (plasmid)
Selectable markers Ampicillin
Gene/insert name human BRD4 cDNA
Depositor|Developer Kanemaki, Masato |
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External Database
human BRD4

          Reference sequence
            
           
          Sequence (full) RDB18359zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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          Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
          Remarks
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word]
          備考
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB18359 BRD4-N CRISPR DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18359_B0Gfp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: SV40pA3element Reverse
          Region: U6 pro,sgRNA for BRD4-N,CBh pro
          Sequence file: RDB18359_B0Gfa.seq check
          >D07105C9_B0Gf_3_SV40pA3'element Reverse_A05_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNGNNNN NANGGNNNNN NCNNGCAACG CGGCCTTTTT ACGGTTCCTG
             61 GCCTTTTNNN NNNNNNTTGC TCACATGTGA GGGCCTATTT CCCATGATTC CNTCNTATTT
            121 GCATATACGA TACAAGGCTG TTAGAGAGAT AATTGGAATT AATTTGACTG TAAACACAAA
            181 GATATTAGTA CAAAATACGT GACGTAGAAA GTAATAATTT CTTGGGTAGT TTGCAGTTTT
            241 AAAATTATGT TTTAAAATGG ACTATCATAT GCTTACCGTA ACTTGAAAGT ATTTCGATTT
            301 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAACACCGAT GTCTGCGGAG AGCGGCCCGT
            361 TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG TTATCAACTT GAAAAAGTGG
            421 CACCGAGTCG GTGCTTTTTT GTTTTAGAGC TAGAAATAGC AAGTTAAAAT AAGGCTAGTC
            481 CGTTTTTAGC GCGTGCGCCA ATTCTGCAGA CAAATGGCTC TAGAGGTACC CGTTACATAA
            541 CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA
            601 GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC
            661 CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC
            721 GGTAAATGGC CCGCCTGGCA TTGTGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG
            781 CAGTACATCT ACGTATTANT CATCGCTATT ACCATGGTCG ANGTGANCCC CACGTTCTGC
            841 TTCACTCTCC CCATCTCCCC CCCCNNNCCN ACCCCCAATT TTGTANTTAT TTATTTTTTN
          901 NATTATTTTG TGCANCGATG GGGGCGNNNN NNNNNN
          //
          Primer: bGH_rev3
          Region: nucleoplasmin NLS,Cas9
          Sequence file: RDB18359_B0Gfb.seq check
          >D07105C9_B0Gf_3_bGH_rev3_G01_07_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNGGGNNGG NNNNNNACNG ANGGCTGGCA ACTAGAAGGC
             61 ACAGTCGAGG CTGATCAGCN AGCTCTAGGA ATTCTTACTT TTTCTTTTTT GCCTGGCCGG
            121 CCTTTTTCGT GGCCGCCGGC CTTTTGTCGC CTCCCAGCTG AGACAGGTCG ATCCGTGTCT
            181 CGTACAGGCC GGTGATGCTC TGGTGGATCA GGGTGGCGTC CAGCACCTCT TTGGTGCTGG
            241 TGTACCTCTT CCGGTCGATG GTGGTGTCAA AGTACTTGAA GGCGGCAGGG GCTCCCAGAT
            301 TGGTCAGGGT AAACAGGTGG ATGATATTCT CGGCCTGCTC TCTGATGGGC TTATCCCGGT
            361 GCTTGTTGTA GGCGGACAGC ACTTTGTCCA GATTAGCGTC GGCCAGGATC ACTCTCTTGG
            421 AGAACTCGCT GATCTGCTCG ATGATCTCGT CCAGGTAGTG CTTGTGCTGT TCCACAAACA
            481 GCTGTTTCTG CTCATTATCC TCGGGGGAGC CCTTCAGCTT CTCATAGTGG CTGGCCAGGT
            541 ACAGGAAGTT CACATATTTG GAGGGCAGGG CCAGTTCGTT TCCCTTCTGC AGTTCGCCGG
            601 CAGAGGCCAG CATTCTCTTC CGGCCGTTTT CCAGCTCGAA CAGGGAGTAC TTAGGCAGCT
            661 TGATGATCAG GTCCTTTTTC ACTTCTTTGT AGCCCTTGGC TTCCAGAAAG TCGATGGGAT
            721 TCTTCTCGAA NCTGCTTCTT TCCATGATGG TGATCCCCAG CAGCTCTTTC ACACTCTTCA
            781 NTTTCTTGGA CTTGCCCTTT TCCACTTTGG NCNCCACNNN NNNNNAATAN GCCACGGTGG
            841 GGCTGTCNNN NNNNCNNNAC TTCTTAGGGT CCCAGTCCTT CTTTCTGGCG ATNANCTTAN
            901 CNCTGNTCCT CNTGNNNNNG ANANANTCTT TNCTNNANNC CNCNTGNCNG NNNNTCNGNN
            961 CNTTTTCNCN ATATTCACNN NGGGNNNNGC TNNANNNNNT TCCNCNNGGN GGNCNAANNC
           1021 CCGGNCCNTN ATCCCNCNNN NATNNNCCNN NTTNCNNNGT NNGNCNNGAT NNNANNNNNN
          1081 NNCNNNNNNN NNN
          //
          Primer: Cas9-N_R1
          Region: CBh pro,3xFLAG, SV40 NLS,Cas9
          Sequence file: RDB18359_B0Gfc.seq check
          >D07105C9_B0Gf_3_Cas9-N_R3_H01_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNGNCGA NGTCNNGGCC GANGCTGTAC TTCTTGTCGG
             61 CTGCTGGGAC TCCGTGGATA CCGACCTTCC GCTTCTTCTT TGGGGCCATC TTATCGTCAT
            121 CGTCTTTGTA ATCAATATCA TGATCCTTGT AGTCTCCGTC GTGGTCCTTA TAGTCCATGG
            181 TGGCACCGGT CCAACCTGAA AAAAAGTGAT TTCAGGCAGG TGCTCCAGGT AATTAAACAT
            241 TAATACCCCA CCAACCAACC ATCCCTTAAA CCCTTACCTC TTGCTCAGCT AATTACAGCC
            301 CGGAGGAGAA GGGCCGTCCC GCCCGCTCAC CTGTGGGAGT AACGCGGTCA GTCAGAGCCG
          361 GGGCGGGCGG CGCGAGG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

          2022.09.29

          GNP_filter3_RDBDEP_html_220812.pl