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pAY15

TOL2 expression vector of OsTIR1(F74G) and mAID-EGFP-NLS marker.

Catalog number RDB18334
Resource name pAY15
Alternative name OsTIR1(F74G) mAID-EGFP-NLS
Clone info. TOL2 expression vector of OsTIR1(F74G) and mAID-EGFP-NLS marker, see Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone pT2AL200R175-CAGGS-EGFP (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell).
Gene/insert name Oryza sativa OsTIR1 cDNA Arabidopsis thaliana IAA17 cDNA Aequorea victoria GFP (EGFP) cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Oryza sativa OsTIR1 Arabidopsis thaliana IAA17 Aequorea victoria GFP (EGFP)

        Reference sequence
          
         
        Sequence (full) RDB18334zzk01.seq provided by the depositor

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
        Additional terms and conditions for distribution The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Koichi Kawakami of the National Institute of Genetics (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004))is requested.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        提供案内 (日本国内) [open/close]

        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
        付加的提供条件 Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。利用者は、研究成果の公表にあたって国立遺伝学研究所 川上浩一博士が指定する文献 (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004)) の引用を必要とする。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB18334 pAY15 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Original reference

        original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Auxin Inducible Degron (AID) System (English text)
        Featured content Auxin Inducible Degron (AID) System (Japanese text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB18334_B0Gfp1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: pUC-M13_-100
        Region: Tol2R175,insert 3'(lox P,mouse PGK ter)
        Sequence file: RDB18334_B0Gfa.seq check
        >D07105A2B0Gf_1_pUC-M13_-100_E10_13_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGGGTTTT CCCNGTCNCG ACGTTGTAAA
           61 ACGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGG TACCGGCATA
          121 TGGTTCTTGA CAGAGGTGTA AAAAGTACTC AAAAATTTTA CTCAAGTGAA AGTACAAGTA
          181 CTTAGGGAAA ATTTTACTCA ATTAAAAGTA AAAGTATCTG GCTAGAATCT TACTTGAGTA
          241 AAAGTAAAAA AGTACTCCAT TAAAATTGTA CTTGAGTATT AAGGAAGTAA AAGTAAAAGC
          301 AAGAAAGATC TTAGTGAACC TCTTCGAGGG ACCTAATAAC TTCGTATAGC ATACATTATA
          361 CGAAGTTATA TTAAGGGTTC CGGATCTCGA CCAGCTTCTG ATGGAATTAG AACTTGGCAA
          421 AACAATACTG AGAATGAAGT GTATGTGGAA CAGAGGCTGC TGATCTCGTT CTTCAGGCTA
          481 TGAAACTGAC ACATTTGGAA ACCACAGTAC TTAGAACCAC AAAGTGGGAA TCAAGAGAAA
          541 AACAATGATC CCACGAGAGA TCTATAGATC TATAGATCAT GAGTGGGAGG AATGAGCTGG
          601 CCCTTAATTT GGTTTTGCTT GTTTAAATTA TGATATCCAA CTATGAAACA TTATCATAAA
          661 GCAATAGTAA AGAGCCTTCA GTAAAGAGCA GGCATTTATC TAATCCCACC CCACCCCCAC
          721 CCCCGTAGCT CCNATCCTTC CATTCAAAAT GTANGTACTC TGTTCTCACC CTTCTTAACA
          781 AAGTATGACA GGAAAAACTT CCATTTTAGT GGACATCTTT ATTGTTTAAT AGATCATCAA
          841 TTTCTGCATN CCGGGGNNCT GANATCATCG ATGCATGGGG GTCGTGCGCN CCTTTCGGNC
          901 GGGCGCTGCN GNTCGTGGGG GNGGGNNTCN NGNACNNGGN TNGCGGGNCN TGCACNNGGT
          961 GCGCNGNNCC TTCNGNNNNC NCNNCGNNNG NGGNGNNGGN NNANNCNNAN CNNNNCNNAN
        1021 NNNNNANNNN NNGGGGNNCN NNNNNNNNN
        //
        Primer: EGFP-C2
        Region: insert mid(EGFP 3',NLS,SV40pA,lox P,mouse PGK Pro)
        Sequence file: RDB18334_B0Gfb.seq check
        >D07105A2B0Gf_1_EGFP-C2_C10_07_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNCNN NGNNCNCNTG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG
           61 ATCACTCTCG GCATGGNCGA GCTGTACAAG GGTGCAGGCG CCCAAGCTTC GAATTCTGCA
          121 GTCGACCCCA AGAAGAAAAG AAAGGTCATC GACGGTACCG CGGGCCCGGG ATCCACCGGA
          181 TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA
          241 AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC
          301 TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT
          361 AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAA
          421 GCGATCATAT TCAATAACCC TTAATATAAC TTCGTATAAT GTATGCTATA CGAAGTTATT
          481 AGGTCTGAAG AGGAGTTTAC GTCCAGCCAA GCTTAGGATC TCGACCTCGA AATTCTACCG
          541 GGTAGGGGAG GCGCTTTTCC CAAGGCAGTC TGGAGCATGC GCTTTAGCAG CCCCGCTGGG
          601 CACTTGGCGC TACACAAGTG GCCTCTGGCC TCGCACACAT TCCACATCCA CCGGTAGGCG
          661 CCAACCGACT CCGTTCTTTG GTGGCCCCTT CGCGCCACCT TCTACTCCTC CCCTAGTCAG
          721 GAAGTTCCCC CCCGCCCCGC AGCTCGCGTC GTGCANGACG TGACAAATGG AAGTAGCACG
          781 TCTCACTAGT CTCGTGCANA TGGACAGCAC CGCTGANCAA TGGAAGCGGG TANGCCTTTG
          841 GGGCANCGGC CNATANCAGC TTTGCTNCTT CGCTTTCTGG GCTCNNANGC TGGGAANGGG
          901 NGGGTCCGGG GGCGGGCNNN NNNGCGGGNT CNNNNNCNGG GCGGGCGNCN NAANGNCCNC
          961 NNGANNNNNN GNATNCTGNA CGCNNNAAAA NNNNNCGTCN GNCNCGCTNT TNNNNNNNNN
        1021 N
        //
        Primer: EGFP-N_R2
        Region: insert mid(EGFP 5',mAID,P2A,OsTIR1(F74G) 3')
        Sequence file: RDB18334_B0Gfc.seq check
        >D07105A2B0Gf_1_EGFP-N_R2_B10_04_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNAN CNNNNCNCGC CGNANGTCNG GGNGGTCACG
           61 AGGGTGGGCC AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG
          121 CCGTAGGTGG CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG
          181 CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC
          241 ATGCTAGCTT TATACATCCT CAAATCGATT TTCCTCAAGT ACGGTGCTCC GTCCATTGAT
          301 ACCTTCACGA ACGCCGCCGC CTCCGGGCCA CCGCTTGATT TTTGGCAGGA AACCATCACG
          361 TTCTTCCGGT ATGATCTCAC CGGTGGCCAT CCCACAACTT GTGCCTTGGC CGGAGGTTTG
          421 GCTGGATCTT TAGGACAAGC ACTCTTCTCC TTAGATCTAG GTCCAGGGTT CTCCTCCACG
          481 TCTCCAGCCT GCTTCAGCAG GCTGAAGTTA GTAGCTCCGC TTCCTGATCT CAGAATCTTC
          541 ACAAAGTTGG GAGCATCATC TCTTGCCCCT GCGGTGGTCC GATACACATA CAGTTTCTCC
          601 ACCTTTGGCA GGTCGCCATG ATTTTCCTCC ATTTCGTTAG ACCCGTCCCG CTCATTAATG
          661 ACTTCCACGT TCAGCATAGG CATTTTACTA GCCAGGACCT GACAGCCCTT CAGAGTCACA
          721 TTGCAACTAG ACATCCACAG GCTCCTCATT GTCTCGTATC TGGCAAAGTT TCCCAGCAGA
          781 NCGGCATCCC CGAAAGGGCT GTCCCGGANT TCCNGTTTTC GCANANTCTT ACACCCGTTC
          841 ATCACGTGCA TCATGCCNTT ATCGGANTCT NCGGCAAATG CAANGNTCAN CATCTCCAGC
          901 TGCTTANCAT ACTTGNCNAN GTACATGAAC ACTTNGTCTG NCAGCNNGNC NGAAANGNTC
          961 NNCCNCNGGA NTCCCTTGNN NTNTNNCACG ANAGCNNCNA AACCNNTCNT NCNGNGNNTG
        1021 GGNNGNGANN NNNNNNNN
        //
        Primer: pCAG-F_V3
        Region: chimeric inton,insert 5'(OsTIR1(F74G) 5')
        Sequence file: RDB18334_B0Gfd.seq check
        >D07105A2B0Gf_1_pCAG-F_V3_A10_01_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGGNANG GCGGGGTTCG GCTTCTGGCG
           61 TGTGANCGGC GGCTCTAGAG CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA
          121 GCTCCTGGGC AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTCGAGCT
          181 CGGTACCTCG CGAATGCATC TAGATAGCGC TGCCACCATG ACATACTTTC CTGAAGAGGT
          241 CGTCGAACAC ATTTTTAGCT TCCTGCCTGC ACAGAGAGAT AGAAACACAG TGAGCCTGGT
          301 CTGCAAAGTG TGGTACGAGA TCGAACGCCT GAGCCGGAGA GGAGTGTTCG TCGGCAACTG
          361 CTATGCTGTG AGAGCAGGCA GGGTCGCCGC TAGGTTTCCA AATGTGCGCG CACTGACCGT
          421 CAAGGGGAAA CCCCACGGCG CCGACTTTAA CCTGGTGCCC CCTGATTGGG GAGGATACGC
          481 CGGCCCTTGG ATCGAGGCAG CCGCTCGCGG CTGTCATGGA CTGGAGGAAC TGCGCATGAA
          541 GCGAATGGTG GTCTCTGACG AAAGTCTGGA GCTGCTGGCT CGGAGCTTCC CTAGGTTTCG
          601 CGCACTGGTG CTGATTTCTT GCGAAGGCTT CAGCACCGAT GGACTGGCAG CCGTGGCCTC
          661 CCACTGTAAG CTGCTGCGGG AGCTGGACCT CCAGGAGAAT GAAGTGNAGG ATAGAGGCCC
          721 CAGATGGCTG TCTTGCTTCC CAGACTCATG TACCAGCCTG GTGTCCCTGA ACTTTGCCTG
          781 CATCAAAGGC GAAGTGAATG CTGGGTCCCT GNANCGGCTG GTCTCNAGAN NCCCCAACCT
          841 GANGTCTCTG CGGCTGANCN GGANCGTGAG CGNGGACACT CTGGCTAANA NNCTGCTGAN
          901 AACCCCTNNN NTGNANGATC TGGGNANCNG GCNNTCTGAN NNANNATTTN CNNANNNANT
          961 CCTANTTTNA NCTGANTTCT GNCNNNNNNA ANTGTAANNN GNTNNNNNNC TGNCNNNATT
        1021 CNGGGNNGNN TNCNCCNNNN NNNCNNNNNN NTNNNNNNNN NN
        //
        Primer: PGKpro_F
        Region: PuroR 5'
        Sequence file: RDB18334_B0Gfe.seq check
        >18334_18334_B1KhF_1_PGKpro_F_F01_06_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNGCCTTTCG ACCTGCATCN NNTCTNNGAT CTCGAGCAGC
           61 TGAAGCTTAC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC
          121 CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG
          181 TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG
          241 TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA
          301 CCACGCCGGA GAGCGTCNAA NCGGGGGCGG TGTTCGCCGA NATCGGNNNN NGNNNGGNCG
          361 AGTTGAGCGG TTCCCGGCTG GNNGCGNNNN NNNANANGGA ANGNCTCCTG NNGNCNNANC
        421 GNNNNNNNGN NNCNNNNNGG TTN
        //
        Primer: reverse-2
        Region: TolL200,CMV en
        Sequence file: RDB18334_B0Gff.seq check
        >D07105A2B0Gf_1_reverse-2_D10_10_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CNNNNTANNN NNNNCTANNN NNNNNNNNAA
           61 GCTGGAGCTC GGATCTGCTG GGCTTGCTGA AGGTAGGGGG TCAAGAACCA GAGGTGTAAA
          121 GTACTTGAGT AATTTTACTT GATTACTGTA CTTAAGTATT ATTTTTGGGG ATTTTTACTT
          181 TACTTGAGTA CAATTAAAAA TCAATACTTT TACTTTTACT TAATTACATT TTTTTAGAAA
          241 AAAAAGTACT TTTTACTCCT TACAATTTTA TTTACAGTCA AAAAGTACTT ATTTTTTGGA
          301 GATCACTTGG GCCCTCTAGA TGCATGCTCG ACATTGATTA TTGACTAGTT ATTAATAGTA
          361 ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC
          421 GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC
          481 GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT
          541 ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT
          601 TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA
          661 CTTTCCTACT TGGCAGTACA TCTACGTATT ANNCATCGNN NNNACCATGG TCGAGGTGAG
          721 CCCCACGTTC TGCTTCACTC TCCCCCATCT CCCCCCCCTC CCCACCCCCA ATTTTGTATT
          781 TATTTATTTT TNAATTATTT TGNGCANCGA TGGGGGCGGG GGGGGGGGGG GGNNNNNNNN
        841 NN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522.

        2022.08.12

        GNP_filter3_RDBDEP_html_220812.pl