RDB18334 - pAY15

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pAY15 (#RDB18334)

TOL2 expression vector of OsTIR1(F74G) and mAID-EGFP-NLS marker.

Alternative name	OsTIR1(F74G) mAID-EGFP-NLS
Clone info.	TOL2 expression vector of OsTIR1(F74G) and mAID-EGFP-NLS marker, see Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone	pT2AL200R175-CAGGS-EGFP (plasmid)
Selectable markers	Ampicillin (E. coli), Puromycin (mammalian cell).
Gene/insert name	Oryza sativa OsTIR1 cDNA Arabidopsis thaliana IAA17 cDNA Aequorea victoria GFP (EGFP) cDNA
Depositor\|Developer	Kanemaki, Masato \|

Sequence (full)	RDB18334zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
Additional terms and conditions:
The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Koichi Kawakami of the National Institute of Genetics (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004))is requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)). Additional terms and conditions: The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Koichi Kawakami of the National Institute of Genetics (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004))is requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。 MTAに書く付加的使用条件： Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。利用者は、研究成果の公表にあたって国立遺伝学研究所川上浩一博士が指定する文献 (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004)) の引用を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
MTAに書く付加的使用条件：
Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。利用者は、研究成果の公表にあたって国立遺伝学研究所川上浩一博士が指定する文献 (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004)) の引用を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18334	pAY15	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAY15 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18334).

Reference section:

Yesbolatova, A., Saito, Y., Kitamoto, N., Makino-Itou, H., Ajima, R., Nakano, R., Nakaoka, H., Fukui, K., Gamo, K., Tominari, Y., Takeuchi, H., Saga, Y., Hayashi, K., Kanemaki, M.T., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18334_B0Gfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310) Region: Tol2R175,insert 3'(lox P,mouse PGK ter) Sequence file: RDB18334_B0Gfa.seq
>D07105A2B0Gf_1_pUC-M13_-100_E10_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGGGTTTT CCCNGTCNCG ACGTTGTAAA 61 ACGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGG TACCGGCATA 121 TGGTTCTTGA CAGAGGTGTA AAAAGTACTC AAAAATTTTA CTCAAGTGAA AGTACAAGTA 181 CTTAGGGAAA ATTTTACTCA ATTAAAAGTA AAAGTATCTG GCTAGAATCT TACTTGAGTA 241 AAAGTAAAAA AGTACTCCAT TAAAATTGTA CTTGAGTATT AAGGAAGTAA AAGTAAAAGC 301 AAGAAAGATC TTAGTGAACC TCTTCGAGGG ACCTAATAAC TTCGTATAGC ATACATTATA 361 CGAAGTTATA TTAAGGGTTC CGGATCTCGA CCAGCTTCTG ATGGAATTAG AACTTGGCAA 421 AACAATACTG AGAATGAAGT GTATGTGGAA CAGAGGCTGC TGATCTCGTT CTTCAGGCTA 481 TGAAACTGAC ACATTTGGAA ACCACAGTAC TTAGAACCAC AAAGTGGGAA TCAAGAGAAA 541 AACAATGATC CCACGAGAGA TCTATAGATC TATAGATCAT GAGTGGGAGG AATGAGCTGG 601 CCCTTAATTT GGTTTTGCTT GTTTAAATTA TGATATCCAA CTATGAAACA TTATCATAAA 661 GCAATAGTAA AGAGCCTTCA GTAAAGAGCA GGCATTTATC TAATCCCACC CCACCCCCAC 721 CCCCGTAGCT CCNATCCTTC CATTCAAAAT GTANGTACTC TGTTCTCACC CTTCTTAACA 781 AAGTATGACA GGAAAAACTT CCATTTTAGT GGACATCTTT ATTGTTTAAT AGATCATCAA 841 TTTCTGCATN CCGGGGNNCT GANATCATCG ATGCATGGGG GTCGTGCGCN CCTTTCGGNC 901 GGGCGCTGCN GNTCGTGGGG GNGGGNNTCN NGNACNNGGN TNGCGGGNCN TGCACNNGGT 961 GCGCNGNNCC TTCNGNNNNC NCNNCGNNNG NGGNGNNGGN NNANNCNNAN CNNNNCNNAN 1021 NNNNNANNNN NNGGGGNNCN NNNNNNNNN //
Primer: EGFP-C2 (Pr0402) Region: insert mid(EGFP 3',NLS,SV40pA,lox P,mouse PGK Pro) Sequence file: RDB18334_B0Gfb.seq
>D07105A2B0Gf_1_EGFP-C2_C10_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNCNN NGNNCNCNTG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG 61 ATCACTCTCG GCATGGNCGA GCTGTACAAG GGTGCAGGCG CCCAAGCTTC GAATTCTGCA 121 GTCGACCCCA AGAAGAAAAG AAAGGTCATC GACGGTACCG CGGGCCCGGG ATCCACCGGA 181 TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA 241 AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC 301 TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT 361 AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAA 421 GCGATCATAT TCAATAACCC TTAATATAAC TTCGTATAAT GTATGCTATA CGAAGTTATT 481 AGGTCTGAAG AGGAGTTTAC GTCCAGCCAA GCTTAGGATC TCGACCTCGA AATTCTACCG 541 GGTAGGGGAG GCGCTTTTCC CAAGGCAGTC TGGAGCATGC GCTTTAGCAG CCCCGCTGGG 601 CACTTGGCGC TACACAAGTG GCCTCTGGCC TCGCACACAT TCCACATCCA CCGGTAGGCG 661 CCAACCGACT CCGTTCTTTG GTGGCCCCTT CGCGCCACCT TCTACTCCTC CCCTAGTCAG 721 GAAGTTCCCC CCCGCCCCGC AGCTCGCGTC GTGCANGACG TGACAAATGG AAGTAGCACG 781 TCTCACTAGT CTCGTGCANA TGGACAGCAC CGCTGANCAA TGGAAGCGGG TANGCCTTTG 841 GGGCANCGGC CNATANCAGC TTTGCTNCTT CGCTTTCTGG GCTCNNANGC TGGGAANGGG 901 NGGGTCCGGG GGCGGGCNNN NNNGCGGGNT CNNNNNCNGG GCGGGCGNCN NAANGNCCNC 961 NNGANNNNNN GNATNCTGNA CGCNNNAAAA NNNNNCGTCN GNCNCGCTNT TNNNNNNNNN 1021 N //
Primer: EGFP-N_R2 (Pr0905) Region: insert mid(EGFP 5',mAID,P2A,OsTIR1(F74G) 3') Sequence file: RDB18334_B0Gfc.seq
>D07105A2B0Gf_1_EGFP-N_R2_B10_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNAN CNNNNCNCGC CGNANGTCNG GGNGGTCACG 61 AGGGTGGGCC AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG 121 CCGTAGGTGG CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG 181 CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC 241 ATGCTAGCTT TATACATCCT CAAATCGATT TTCCTCAAGT ACGGTGCTCC GTCCATTGAT 301 ACCTTCACGA ACGCCGCCGC CTCCGGGCCA CCGCTTGATT TTTGGCAGGA AACCATCACG 361 TTCTTCCGGT ATGATCTCAC CGGTGGCCAT CCCACAACTT GTGCCTTGGC CGGAGGTTTG 421 GCTGGATCTT TAGGACAAGC ACTCTTCTCC TTAGATCTAG GTCCAGGGTT CTCCTCCACG 481 TCTCCAGCCT GCTTCAGCAG GCTGAAGTTA GTAGCTCCGC TTCCTGATCT CAGAATCTTC 541 ACAAAGTTGG GAGCATCATC TCTTGCCCCT GCGGTGGTCC GATACACATA CAGTTTCTCC 601 ACCTTTGGCA GGTCGCCATG ATTTTCCTCC ATTTCGTTAG ACCCGTCCCG CTCATTAATG 661 ACTTCCACGT TCAGCATAGG CATTTTACTA GCCAGGACCT GACAGCCCTT CAGAGTCACA 721 TTGCAACTAG ACATCCACAG GCTCCTCATT GTCTCGTATC TGGCAAAGTT TCCCAGCAGA 781 NCGGCATCCC CGAAAGGGCT GTCCCGGANT TCCNGTTTTC GCANANTCTT ACACCCGTTC 841 ATCACGTGCA TCATGCCNTT ATCGGANTCT NCGGCAAATG CAANGNTCAN CATCTCCAGC 901 TGCTTANCAT ACTTGNCNAN GTACATGAAC ACTTNGTCTG NCAGCNNGNC NGAAANGNTC 961 NNCCNCNGGA NTCCCTTGNN NTNTNNCACG ANAGCNNCNA AACCNNTCNT NCNGNGNNTG 1021 GGNNGNGANN NNNNNNNN //
Primer: pCAG-F_V3 (Pr0495) Region: chimeric inton,insert 5'(OsTIR1(F74G) 5') Sequence file: RDB18334_B0Gfd.seq
>D07105A2B0Gf_1_pCAG-F_V3_A10_01_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGGNANG GCGGGGTTCG GCTTCTGGCG 61 TGTGANCGGC GGCTCTAGAG CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA 121 GCTCCTGGGC AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTCGAGCT 181 CGGTACCTCG CGAATGCATC TAGATAGCGC TGCCACCATG ACATACTTTC CTGAAGAGGT 241 CGTCGAACAC ATTTTTAGCT TCCTGCCTGC ACAGAGAGAT AGAAACACAG TGAGCCTGGT 301 CTGCAAAGTG TGGTACGAGA TCGAACGCCT GAGCCGGAGA GGAGTGTTCG TCGGCAACTG 361 CTATGCTGTG AGAGCAGGCA GGGTCGCCGC TAGGTTTCCA AATGTGCGCG CACTGACCGT 421 CAAGGGGAAA CCCCACGGCG CCGACTTTAA CCTGGTGCCC CCTGATTGGG GAGGATACGC 481 CGGCCCTTGG ATCGAGGCAG CCGCTCGCGG CTGTCATGGA CTGGAGGAAC TGCGCATGAA 541 GCGAATGGTG GTCTCTGACG AAAGTCTGGA GCTGCTGGCT CGGAGCTTCC CTAGGTTTCG 601 CGCACTGGTG CTGATTTCTT GCGAAGGCTT CAGCACCGAT GGACTGGCAG CCGTGGCCTC 661 CCACTGTAAG CTGCTGCGGG AGCTGGACCT CCAGGAGAAT GAAGTGNAGG ATAGAGGCCC 721 CAGATGGCTG TCTTGCTTCC CAGACTCATG TACCAGCCTG GTGTCCCTGA ACTTTGCCTG 781 CATCAAAGGC GAAGTGAATG CTGGGTCCCT GNANCGGCTG GTCTCNAGAN NCCCCAACCT 841 GANGTCTCTG CGGCTGANCN GGANCGTGAG CGNGGACACT CTGGCTAANA NNCTGCTGAN 901 AACCCCTNNN NTGNANGATC TGGGNANCNG GCNNTCTGAN NNANNATTTN CNNANNNANT 961 CCTANTTTNA NCTGANTTCT GNCNNNNNNA ANTGTAANNN GNTNNNNNNC TGNCNNNATT 1021 CNGGGNNGNN TNCNCCNNNN NNNCNNNNNN NTNNNNNNNN NN //
Primer: PGKpro_F (Pr0249) Region: PuroR 5' Sequence file: RDB18334_B0Gfe.seq
>18334_18334_B1KhF_1_PGKpro_F_F01_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGCCTTTCG ACCTGCATCN NNTCTNNGAT CTCGAGCAGC 61 TGAAGCTTAC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC 121 CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG 181 TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG 241 TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA 301 CCACGCCGGA GAGCGTCNAA NCGGGGGCGG TGTTCGCCGA NATCGGNNNN NGNNNGGNCG 361 AGTTGAGCGG TTCCCGGCTG GNNGCGNNNN NNNANANGGA ANGNCTCCTG NNGNCNNANC 421 GNNNNNNNGN NNCNNNNNGG TTN //
Primer: reverse-2 (Pr0100) Region: TolL200,CMV en Sequence file: RDB18334_B0Gff.seq
>D07105A2B0Gf_1_reverse-2_D10_10_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CNNNNTANNN NNNNCTANNN NNNNNNNNAA 61 GCTGGAGCTC GGATCTGCTG GGCTTGCTGA AGGTAGGGGG TCAAGAACCA GAGGTGTAAA 121 GTACTTGAGT AATTTTACTT GATTACTGTA CTTAAGTATT ATTTTTGGGG ATTTTTACTT 181 TACTTGAGTA CAATTAAAAA TCAATACTTT TACTTTTACT TAATTACATT TTTTTAGAAA 241 AAAAAGTACT TTTTACTCCT TACAATTTTA TTTACAGTCA AAAAGTACTT ATTTTTTGGA 301 GATCACTTGG GCCCTCTAGA TGCATGCTCG ACATTGATTA TTGACTAGTT ATTAATAGTA 361 ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC 421 GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC 481 GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT 541 ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT 601 TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA 661 CTTTCCTACT TGGCAGTACA TCTACGTATT ANNCATCGNN NNNACCATGG TCGAGGTGAG 721 CCCCACGTTC TGCTTCACTC TCCCCCATCT CCCCCCCCTC CCCACCCCCA ATTTTGTATT 781 TATTTATTTT TNAATTATTT TGNGCANCGA TGGGGGCGGG GGGGGGGGGG GGNNNNNNNN 841 NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]
user_report	Kishimoto, T., An engineered ligand-responsive Csy4 endoribonuclease controls transgene expression from Sendai virus vectors. J. Biol. Eng. 18 (1): 9 (2024). PMID 38229076. [link to RRC of NBRP]