Please review the QC test results indicated by icon below as well as clone information before placing your order.

pAY15

TOL2 expression vector of OsTIR1(F74G) and mAID-EGFP-NLS marker.

Sequence (full)	RDB18334zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)). Additional terms and conditions: The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Koichi Kawakami of the National Institute of Genetics (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004))is requested.

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Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。 付加的提供条件： Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。利用者は、研究成果の公表にあたって国立遺伝学研究所 川上浩一博士が指定する文献 (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004)) の引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18334_B0Gfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310) Region: Tol2R175,insert 3'(lox P,mouse PGK ter) Sequence file: RDB18334_B0Gfa.seq
>D07105A2B0Gf_1_pUC-M13_-100_E10_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGGGTTTT CCCNGTCNCG ACGTTGTAAA    61 ACGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGG TACCGGCATA   121 TGGTTCTTGA CAGAGGTGTA AAAAGTACTC AAAAATTTTA CTCAAGTGAA AGTACAAGTA   181 CTTAGGGAAA ATTTTACTCA ATTAAAAGTA AAAGTATCTG GCTAGAATCT TACTTGAGTA   241 AAAGTAAAAA AGTACTCCAT TAAAATTGTA CTTGAGTATT AAGGAAGTAA AAGTAAAAGC   301 AAGAAAGATC TTAGTGAACC TCTTCGAGGG ACCTAATAAC TTCGTATAGC ATACATTATA   361 CGAAGTTATA TTAAGGGTTC CGGATCTCGA CCAGCTTCTG ATGGAATTAG AACTTGGCAA   421 AACAATACTG AGAATGAAGT GTATGTGGAA CAGAGGCTGC TGATCTCGTT CTTCAGGCTA   481 TGAAACTGAC ACATTTGGAA ACCACAGTAC TTAGAACCAC AAAGTGGGAA TCAAGAGAAA   541 AACAATGATC CCACGAGAGA TCTATAGATC TATAGATCAT GAGTGGGAGG AATGAGCTGG   601 CCCTTAATTT GGTTTTGCTT GTTTAAATTA TGATATCCAA CTATGAAACA TTATCATAAA   661 GCAATAGTAA AGAGCCTTCA GTAAAGAGCA GGCATTTATC TAATCCCACC CCACCCCCAC   721 CCCCGTAGCT CCNATCCTTC CATTCAAAAT GTANGTACTC TGTTCTCACC CTTCTTAACA   781 AAGTATGACA GGAAAAACTT CCATTTTAGT GGACATCTTT ATTGTTTAAT AGATCATCAA   841 TTTCTGCATN CCGGGGNNCT GANATCATCG ATGCATGGGG GTCGTGCGCN CCTTTCGGNC   901 GGGCGCTGCN GNTCGTGGGG GNGGGNNTCN NGNACNNGGN TNGCGGGNCN TGCACNNGGT   961 GCGCNGNNCC TTCNGNNNNC NCNNCGNNNG NGGNGNNGGN NNANNCNNAN CNNNNCNNAN 1021 NNNNNANNNN NNGGGGNNCN NNNNNNNNN //
Primer: EGFP-C2 (Pr0402) Region: insert mid(EGFP 3',NLS,SV40pA,lox P,mouse PGK Pro) Sequence file: RDB18334_B0Gfb.seq
>D07105A2B0Gf_1_EGFP-C2_C10_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCNN NGNNCNCNTG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG    61 ATCACTCTCG GCATGGNCGA GCTGTACAAG GGTGCAGGCG CCCAAGCTTC GAATTCTGCA   121 GTCGACCCCA AGAAGAAAAG AAAGGTCATC GACGGTACCG CGGGCCCGGG ATCCACCGGA   181 TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA   241 AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC   301 TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT   361 AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAA   421 GCGATCATAT TCAATAACCC TTAATATAAC TTCGTATAAT GTATGCTATA CGAAGTTATT   481 AGGTCTGAAG AGGAGTTTAC GTCCAGCCAA GCTTAGGATC TCGACCTCGA AATTCTACCG   541 GGTAGGGGAG GCGCTTTTCC CAAGGCAGTC TGGAGCATGC GCTTTAGCAG CCCCGCTGGG   601 CACTTGGCGC TACACAAGTG GCCTCTGGCC TCGCACACAT TCCACATCCA CCGGTAGGCG   661 CCAACCGACT CCGTTCTTTG GTGGCCCCTT CGCGCCACCT TCTACTCCTC CCCTAGTCAG   721 GAAGTTCCCC CCCGCCCCGC AGCTCGCGTC GTGCANGACG TGACAAATGG AAGTAGCACG   781 TCTCACTAGT CTCGTGCANA TGGACAGCAC CGCTGANCAA TGGAAGCGGG TANGCCTTTG   841 GGGCANCGGC CNATANCAGC TTTGCTNCTT CGCTTTCTGG GCTCNNANGC TGGGAANGGG   901 NGGGTCCGGG GGCGGGCNNN NNNGCGGGNT CNNNNNCNGG GCGGGCGNCN NAANGNCCNC   961 NNGANNNNNN GNATNCTGNA CGCNNNAAAA NNNNNCGTCN GNCNCGCTNT TNNNNNNNNN 1021 N //
Primer: EGFP-N_R2 (Pr0905) Region: insert mid(EGFP 5',mAID,P2A,OsTIR1(F74G) 3') Sequence file: RDB18334_B0Gfc.seq
>D07105A2B0Gf_1_EGFP-N_R2_B10_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNAN CNNNNCNCGC CGNANGTCNG GGNGGTCACG    61 AGGGTGGGCC AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG   121 CCGTAGGTGG CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG   181 CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC   241 ATGCTAGCTT TATACATCCT CAAATCGATT TTCCTCAAGT ACGGTGCTCC GTCCATTGAT   301 ACCTTCACGA ACGCCGCCGC CTCCGGGCCA CCGCTTGATT TTTGGCAGGA AACCATCACG   361 TTCTTCCGGT ATGATCTCAC CGGTGGCCAT CCCACAACTT GTGCCTTGGC CGGAGGTTTG   421 GCTGGATCTT TAGGACAAGC ACTCTTCTCC TTAGATCTAG GTCCAGGGTT CTCCTCCACG   481 TCTCCAGCCT GCTTCAGCAG GCTGAAGTTA GTAGCTCCGC TTCCTGATCT CAGAATCTTC   541 ACAAAGTTGG GAGCATCATC TCTTGCCCCT GCGGTGGTCC GATACACATA CAGTTTCTCC   601 ACCTTTGGCA GGTCGCCATG ATTTTCCTCC ATTTCGTTAG ACCCGTCCCG CTCATTAATG   661 ACTTCCACGT TCAGCATAGG CATTTTACTA GCCAGGACCT GACAGCCCTT CAGAGTCACA   721 TTGCAACTAG ACATCCACAG GCTCCTCATT GTCTCGTATC TGGCAAAGTT TCCCAGCAGA   781 NCGGCATCCC CGAAAGGGCT GTCCCGGANT TCCNGTTTTC GCANANTCTT ACACCCGTTC   841 ATCACGTGCA TCATGCCNTT ATCGGANTCT NCGGCAAATG CAANGNTCAN CATCTCCAGC   901 TGCTTANCAT ACTTGNCNAN GTACATGAAC ACTTNGTCTG NCAGCNNGNC NGAAANGNTC   961 NNCCNCNGGA NTCCCTTGNN NTNTNNCACG ANAGCNNCNA AACCNNTCNT NCNGNGNNTG 1021 GGNNGNGANN NNNNNNNN //
Primer: pCAG-F_V3 (Pr0495) Region: chimeric inton,insert 5'(OsTIR1(F74G) 5') Sequence file: RDB18334_B0Gfd.seq
>D07105A2B0Gf_1_pCAG-F_V3_A10_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGGNANG GCGGGGTTCG GCTTCTGGCG    61 TGTGANCGGC GGCTCTAGAG CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA   121 GCTCCTGGGC AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTCGAGCT   181 CGGTACCTCG CGAATGCATC TAGATAGCGC TGCCACCATG ACATACTTTC CTGAAGAGGT   241 CGTCGAACAC ATTTTTAGCT TCCTGCCTGC ACAGAGAGAT AGAAACACAG TGAGCCTGGT   301 CTGCAAAGTG TGGTACGAGA TCGAACGCCT GAGCCGGAGA GGAGTGTTCG TCGGCAACTG   361 CTATGCTGTG AGAGCAGGCA GGGTCGCCGC TAGGTTTCCA AATGTGCGCG CACTGACCGT   421 CAAGGGGAAA CCCCACGGCG CCGACTTTAA CCTGGTGCCC CCTGATTGGG GAGGATACGC   481 CGGCCCTTGG ATCGAGGCAG CCGCTCGCGG CTGTCATGGA CTGGAGGAAC TGCGCATGAA   541 GCGAATGGTG GTCTCTGACG AAAGTCTGGA GCTGCTGGCT CGGAGCTTCC CTAGGTTTCG   601 CGCACTGGTG CTGATTTCTT GCGAAGGCTT CAGCACCGAT GGACTGGCAG CCGTGGCCTC   661 CCACTGTAAG CTGCTGCGGG AGCTGGACCT CCAGGAGAAT GAAGTGNAGG ATAGAGGCCC   721 CAGATGGCTG TCTTGCTTCC CAGACTCATG TACCAGCCTG GTGTCCCTGA ACTTTGCCTG   781 CATCAAAGGC GAAGTGAATG CTGGGTCCCT GNANCGGCTG GTCTCNAGAN NCCCCAACCT   841 GANGTCTCTG CGGCTGANCN GGANCGTGAG CGNGGACACT CTGGCTAANA NNCTGCTGAN   901 AACCCCTNNN NTGNANGATC TGGGNANCNG GCNNTCTGAN NNANNATTTN CNNANNNANT   961 CCTANTTTNA NCTGANTTCT GNCNNNNNNA ANTGTAANNN GNTNNNNNNC TGNCNNNATT 1021 CNGGGNNGNN TNCNCCNNNN NNNCNNNNNN NTNNNNNNNN NN //
Primer: PGKpro_F (Pr0249) Region: PuroR 5' Sequence file: RDB18334_B0Gfe.seq
>18334_18334_B1KhF_1_PGKpro_F_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGCCTTTCG ACCTGCATCN NNTCTNNGAT CTCGAGCAGC    61 TGAAGCTTAC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC   121 CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG   181 TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG   241 TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA   301 CCACGCCGGA GAGCGTCNAA NCGGGGGCGG TGTTCGCCGA NATCGGNNNN NGNNNGGNCG   361 AGTTGAGCGG TTCCCGGCTG GNNGCGNNNN NNNANANGGA ANGNCTCCTG NNGNCNNANC 421 GNNNNNNNGN NNCNNNNNGG TTN //
Primer: reverse-2 (Pr0100) Region: TolL200,CMV en Sequence file: RDB18334_B0Gff.seq
>D07105A2B0Gf_1_reverse-2_D10_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CNNNNTANNN NNNNCTANNN NNNNNNNNAA    61 GCTGGAGCTC GGATCTGCTG GGCTTGCTGA AGGTAGGGGG TCAAGAACCA GAGGTGTAAA   121 GTACTTGAGT AATTTTACTT GATTACTGTA CTTAAGTATT ATTTTTGGGG ATTTTTACTT   181 TACTTGAGTA CAATTAAAAA TCAATACTTT TACTTTTACT TAATTACATT TTTTTAGAAA   241 AAAAAGTACT TTTTACTCCT TACAATTTTA TTTACAGTCA AAAAGTACTT ATTTTTTGGA   301 GATCACTTGG GCCCTCTAGA TGCATGCTCG ACATTGATTA TTGACTAGTT ATTAATAGTA   361 ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC   421 GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC   481 GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT   541 ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT   601 TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA   661 CTTTCCTACT TGGCAGTACA TCTACGTATT ANNCATCGNN NNNACCATGG TCGAGGTGAG   721 CCCCACGTTC TGCTTCACTC TCCCCCATCT CCCCCCCCTC CCCACCCCCA ATTTTGTATT   781 TATTTATTTT TNAATTATTT TGNGCANCGA TGGGGGCGGG GGGGGGGGGG GGNNNNNNNN 841 NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl