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pMK381

A donor vector for introducing CMV-OsTIR1 (F74G mutant) at the human AAVS1 locus.

Catalog number RDB18337
Resource name pMK381
Alternative name AAVS1 CMV-OsTIR1 F74G
Clone info. A donor vector for introducing CMV-OsTIR1 (F74G mutant) at the human AAVS1 locus, see Fig. 1B-E, SFig. 1B and SFig. 5 of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone pCR (plasmid)
Selectable markers Ampillin (E. coli), Puromycin (mammalian cell).
Gene/insert name Oryza sativa OsTIR1 cDNA human AAVS1 genomic
Depositor|Developer Kanemaki, Masato |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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RDB18337 pMK381 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence provided by the depositor RDB18337zzk02.seq

Original reference

original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020).

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18337_B0Gfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMVpro, OstIR1(F74G) 5'
Sequence file: RDB18337_B0Gfa.seq check
>D07105A5_B0Gf_2_CMV_Forward_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTT GNNGCNNTGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTGCCACCAT GACATACTTT
  121 CCTGAAGAGG TCGTCGAACA CATTTTTAGC TTCCTGCCTG CACAGAGAGA TAGAAACACA
  181 GTGAGCCTGG TCTGCAAAGT GTGGTACGAG ATCGAACGCC TGAGCCGGAG AGGAGTGTTC
  241 GTCGGCAACT GCTATGCTGT GAGAGCAGGC AGGGTCGCCG CTAGGTTTCC AAATGTGCGC
  301 GCACTGACCG TCAAGGGGAA ACCCCACGGC GCCGACTTTA ACCTGGTGCC CCCTGATTGG
  361 GGAGGATACG CCGGCCCTTG GATCGAGGCA GCCGCTCGCG GCTGTCATGG ACTGGAGGAA
  421 CTGCGCATGA AGCGAATGGT GGTCTCTGAC GAAAGTCTGG AGCTGCTGGC TCGGAGCTTC
  481 CCTAGGTTTC GCGCACTGGT GCTGATTTCT TGCGAAGGCT TCAGCACCGA TGGACTGGCA
  541 GCCGTGGCCT CCCACTGTAA GCTGCTGCGG GAGCTGGACC TCCAGGAGAA TGAAGTGGAG
  601 GATAGAGGCC CCAGATGGCT GTCTTGCTTC CCAGACTCAT GTACCAGCCT GGTGTCCCTG
  661 AACTTTGCCT GCATCAAAGG CGAAGTGAAT GCTGGGTCCC TGGAGCGGCT GGTCTCAAGA
  721 AGCCCCAACC TGAGGTCTCT GCGGCTGAAC CGGAGCGTGA GCGTGGACAC TCTGGCTAAG
  781 ATTCTGCTGA NAACCCCTAA CCTGGANGAT CTGGGAACCG GCAATCTGAC AGANGATTTC
  841 CAGANAGAAT CCTACTTTNA ACTGACTTCT GNCCTGNAGA ANTGNAAAAT GNTGANGANT
  901 CTGTCNNNAT TCTGGNATGC TNCNCCCGNG TGCCTGANCT TTATCTACCC TCTGTGTGCA
  961 CAGCTGANNN NNCTGNACCT GANCTATGCN NNNNNCCNNN NNGNNNNNGN TCNGNNNANN
1021 NNNNTNNNNN NNNNN
//
Primer: PGK_N_rev
Region: loxP, SV40 pA, OsTIR1(F74G) 3'
Sequence file: RDB18337_B0Gfb.seq check
>D07105A5_B0Gf_2_PGK_N_rev_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNANANTTCN AGGTCGAGAT CCTAAGCTTG GCTGGACGTA
   61 AACTCCTCTT CAGACCTAAT AACTTCGTAT AGCATACATT ATACGAAGTT ATATTAAGGG
  121 TTATTGAATA TGATCGCTTA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC
  181 AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA
  241 TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG
  301 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT
  361 ATGATCTAGA GTCGCGGCCC CGGTGGATCC CGGGCCCGCG GTACCGTCGA CGGGCCCGGG
  421 ATCCGATGGA TCCTCACAGA ATCTTCACAA AGTTGGGAGC ATCATCTCTT GCCCCTGCGG
  481 TGGTCCGATA CACATACAGT TTCTCCACCT TTGGCAGGTC GCCATGATTT TCCTCCATTT
  541 CGTTAGACCC GTCCCGCTCA TTAATGACTT CCACGTTCAG CATAGGCATT TTACTAGCCA
  601 GGACCTGACA GCCCTTCAGA GTCACATTGC AACTAGACAT CCACAGGCTC CTCATTGTCT
  661 CGTATCTGGC AAAGTTTCCC AGCAGAGCGG CATCCCCGAA AGGGCTGTCC CGGATTTCCA
  721 GTTTTCGCAG ATTCTTACAC CCGTTCATCA CGTGCATCAT GCCCTTATCG GANTCTCCGG
  781 NAAATGNNAT GNTCAGCATC TCCAGCTGCT TANCATACTT GCCGANGTAC NTGAANACTT
  841 TGTCTGNCAN CANGNCNGAA ATGNTCANCC NCTNGANTCC CTTGNNCTCT CTCNCNATAG
  901 CTNNNAACCC TTCATNCNGN GNNNGGGNNG NNANNNCNNN NGNNTNNNCN NNNNNNNNAN
961 NANNNANNNN AANNNGNNNN ANNNNNGNNN NNNNNNN
//
Primer: reverse-2
Region: AAVS1 arm1
Sequence file: RDB18337_B0Gfc.seq check
>D07105A5_B0Gf_2_reverse-2_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNCCTCN CTAAAGGGNN TAGTCCTGCA
   61 GGTTTAAACG AATTCGCCCT TTGCTTTCTC TGACCAGCAT TCTCTCCCCT GGGCCTGTGC
  121 CGCTTTCTGT CTGCAGCTTG TGGCCTGGGT CACCTCTACG GCTGGCCCAG ATCCTTCCCT
  181 GCCGCCTCCT TCAGGTTCCG TCTTCCTCCA CTCCCTCTTC CCCTTGCTCT CTGCTGTGTT
  241 GCTGCCCAAG GATGCTCTTT CCGGAGCACT TCCTTCTCGG CGCTGCACCA CGTGATGTCC
  301 TCTGAGCGGA TCCTCCCCGT GTCTGGGTCC TCTCCGGGCA TCTCTCCTCC CTCACCCAAC
  361 CCCATGCCGT CTTCACTCGC TGGGTTCCCT TTTCCTTCTC CTTCTGGGGC CTGTGCCATC
  421 TCTCGTTTCT TAGGATGGCC TTCTCCGACG GATGTCTCCC TTGCGTCCCG CCTCCCCTTC
  481 TTGTAGGCCT GCATCATCAC CGTTTTTCTG GACAACCCCA AAGTACCCCG TCTCCCTGGC
  541 TTTAGCCACC TCTCCATCCT CTTGCTTTCT TTGCCTGGAC ACCCCGTTCT CCTGTGGATT
  601 CGGGTCACCT CTCACTCCTT TCATTTGGGC AGCTCCCCTA CCCCCCTTAC CTCNNNNNNC
  661 TGTGCTAGCT CTTCCAGCCC CCTGTCATGG CATCTTCCAG GGGTCCGAGA GCTCAGCTAG
  721 TCTTCTTCCT CCAACCCGGG CCCCTATGTC CACTTCAGGA CAGCATGTTT GCTGCCTCCA
  781 GGGATCCTGT GTCCCCGAGC TGGGANCACC TTATATTCCC ANGGCCGGTT AATGNGGCTC
  841 TGGTTCTGGG TANTTTTATC TGNCCCCTCC ANCCCACANT GGGGNAAGCT ANTAATCAAT
  901 TACGGGGTCA TTAGTTCATA NCCNNTATAT GGAGTTNNCN CGNTACNTNA CTTACNGTAA
  961 ANGGCCCGCC TGGNTGANNG CCCNACNANC CCCGNCNNNT GACNTNANNN TGANGNATGT
1021 TNCNNNNNNN NNCGNCNANN NGGGANTTTC CATTNNNNNN NNNN
//
Primer: pUC-M13_-100
Region: AVVS1 arm2
Sequence file: RDB18337_B0Gfd.seq check
>D07105A5_B0Gf_2_pUC-M13_-100_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TGNGNANGCN NGGNTTTTCC CNGTCNCGAC GTTGTAAAAC
   61 GACGGCCAGT GAATTGTAAT ACGACTCACT ATAGGGCGAA TTGAATTTAG CGGCCGCGAA
  121 TTCGCCCTTA GAGCAGAGCC AGGAACCCCT GTAGGGAAGG GGCAGGAGAG CCAGGGGCAT
  181 GAGATGGTGG ACGAGGAAGG GGGACAGGGA AGCCTGAGCG CCTCTCCTGG GCTTGCCAAG
  241 GACTCAAACC CAGAAGCCCA GAGCAGGGCC TTAGGGAAGC GGGACCCTGC TCTGGGCGGA
  301 GGAATATGTC CCAGATAGCA CTGGGGACTC TTTAAGGAAA GAAGGATGGA GAAAGAGAAA
  361 GGGAGTAGAG GCGGCCACGA CCTGGTGAAC ACCTAGGACG CACCATTCTC ACAAAGGGAG
  421 TTTTCCACAC GGACACCCCC CTCCTCACCA CAGCCCTGCC AGGACGGGGC TGGCTACTGG
  481 CCTTATCTCA CAGGTAAAAC TGACGCACGG AGGAACAATA TAAATTGGGG ACTAGAAAGG
  541 TGAAGAGCCA AAGTTAGAAC TCAGGACCAA CTTATTCTGA TTTTGTTTTT CCAAACTGCT
  601 TCTCCTCTTG GGAAGTGTAA GGAAGCTGCA GCACCAGGAT CAGTGAAACG CACCAGACAG
  661 CCGCGTCAGA GCAGCTCAGG TTCTGGGAGA GGGTAGCGCA GGGTGGCCAC TGAGAACCGG
  721 GCAGGTCACG CATCCCCCCC TTCCCTCCCA CCCCCTGCCN AGCTCTCCCT CCCAGGATCC
  781 TCTCTGGCTC CATCGTAAGC AAACCTTAGA GGTTCTGGNA AGGAGAGAGA TGGCTCCNGG
  841 AAATGGGGGT GTGTCACCAG ATAANGAATC TGCCTAACAN GANGTGGGGG NTAGANCCAA
  901 TATCNNGAGA NTANGAANGA NGANGNCTAN NGNNGGGGCT TTTCTGNCNC CNATCCTGTC
  961 CCNANNAAAG CTTANNGAAN CTCTNCNANG GANCNAATAN CTTCNNATAG CATACNTTAT
1021 NNNAAGNNNT NNNNNNNNNT NNCNNNNNNN NNN
//
Primer: PGKpro_F
Region: PuroR, mouse PGK ter
Sequence file: RDB18337_B0Gfe.seq check
>D07105A5_B0Gf_2_PGKpro_F_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCN NNNNNCCTTT CGACCTGCAT CCNTCTAGAT
   61 CTCGNAGNCA GCTGAANNCT TACCATNNCC GAGTACAAGC CCACGGTGCG CCTCGCCACC
  121 CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC
  181 ACGCGCCACA CCGTCGATCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC
  241 TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG
  301 GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC
  361 CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC
  421 CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC
  481 GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG
  541 CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC ACAACCTCCC CTTCTACGAG
  601 CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC
  661 ATGACCCGCA AGCCCGGTGC CTGACGCCCG CCCCACGACC CGCAGCGCCC GACCGAAAGG
  721 AGCGCACGAC CCCATGCATC GATGATATCA GATCCCCGGG ATGCAGAAAT TGATGATCTA
  781 TTAAACAATA AANNATGTCC ACTAAAATGG AAGTTTTTCC TGTCATACTT TGTTAAGAAN
  841 GGTGANANNA GAGTANCTAC NTTTTGNATG GAAGGATTGG ANCNNNNGGG NTGGGGGTGG
  901 GGTGGGANTA GANNAANNGN CTGNTCNNNN NNGANNNNNN NNTANNNNNN GCNNNANNNA
961 NNNNGNNNCA TANNNGGNNN NNNTANNTTA ANNNNNNNNN N
//
Primer: mPGKter_F
Region: mouse PGK ter, loxP, AVVS1 arm2
Sequence file: RDB18337_B0Gff.seq check
>D07105A5_B0Gf_2_mPGKter_F_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG NTTTGNNNNG TTCTAATTCN NTCAGAAGCT GGTCGAGATC
   61 CGGAACCCTT AATATAACTT CGTATAATGT ATGCTATACG AAGTTATTAG GTCCCTCGAA
  121 GAGGTTCACT AAGCTTTACT AGGGACAGGA TTGGTGACAG AAAAGCCCCA TCCTTAGGCC
  181 TCCTCCTTCC TAGTCTCCTG ATATTGGGTC TAACCCCCAC CTCCTGTTAG GCAGATTCCT
  241 TATCTGGTGA CACACCCCCA TTTCCTGGAG CCATCTCTCT CCTTGCCAGA ACCTCTAAGG
  301 TTTGCTTACG ATGGAGCCAG AGAGGATCCT GGGAGGGAGA GCTTGGCAGG GGGTGGGAGG
  361 GAAGGGGGGG ATGCGTGACC TGCCCGGTTC TCAGTGGCCA CCCTGCGCTA CCCTCTCCCA
  421 GAACCTGAGC TGCTCTGACG CGGCTGTCTG GTGCGTTTCA CTGATCCTGG TGCTGCAGCT
  481 TCCTTACACT TCCCAAGAGG AGAAGCAGTT TGGAAAAACA AAATCAGAAT AAGTTGGTCC
  541 TGAGTTCTAA CTTTGGCTCT TCACCTTTCT AGTCCCCAAT TTATATTGTT CCTCCGTGCG
  601 TCAGTTTTAC CTGTGAGATA AGGCCAGTAG CCAGCCCCGT CCTGGCAGGG CTGTGGTGAG
  661 GAGGGGGGTG TCCGTGTGGA AAACTCCCTT TGTGAGAATG GTGCGTCCTA GGTGTTCACC
  721 AGGTCGTGGC CGCCTCTACT CCCTTTCTCT TTCTCCATCC TTCTTTCCTT AAAGAGTCCC
  781 CAGTGCTATC TGGGACATAT TCCTCCGCCC AGAGCAGGGT CCCGCTTCCC TAANGNCCTG
  841 CTCTGGGCTT CTGGGTTTGA GTCCTTGGCA AGCCCANGAN ANGCGCTCAN GCTTCCCTGT
  901 CCCCCTTCCT CGNCCACCAT CTCATGNCCC TGGNTCTCCT GNCCCTTCCC TANAGGGGTT
  961 CCNGGNTCTG NTCNNANNGN CGAANTCNCG GNCNNCTAAN NTNNNTTCGN CNTATANNGA
1021 GNCNNANNAN NNNTNNNCNN GNNCNNNNNN TTNNNNNNNN NN
//
Primer: ccdB-C_F
Region: ccdB, NeoR_KanR
Sequence file: RDB18337_B0Gfg.seq check
>D07105A5_B0Gf_2_ccdB-C_F_B03_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN TCNNNNNNNA GCTGGCGCAT GNNGNCCNCC GNNTATGGCN
   61 NNGTGTGCCG GTCTCCGTTA TCGGGGAAGA AGTGGCTGAT CTCAGCCACC GCGAAAATGA
  121 CATCAAAAAC GCCATTAACC TGATGTTCTG GGGAATATAA ATGTCAGGCA TGAGATTATC
  181 AAAAAGGATC TTCACCTAGA TCCTTTTCAC GTAGAAAGCC AGTCCGCAGA AACGGTGCTG
  241 ACCCCGGATG AATGTCAGCT ACTGGGCTAT CTGGACAAGG GAAAACGCAA GCGCAAAGAG
  301 AAAGCAGGTA GCTTGCAGTG GGCTTACATG GCGATAGCTA GACTGGGCGG TTTTATGGAC
  361 AGCAAGCGAA CCGGAATTGC CAGCTGGGGC GCCCTCTGGT AAGGTTGGGA AGCCCTGCAA
  421 AGTAAACTGG ATGGCTTTCT TGCCGCCAAG GATCTGATGG CGCAGGGGAT CAAGCTCTGA
  481 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC
  541 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG
  601 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC
  661 CGACCTGTCC GGTGCCCTGA ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC
  721 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG
  781 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA
  841 GAAAGTATCC ATCATGGCTG ATGCNATGCG GCGGCTGCAT ACGCTTGATC CGGCTACNTG
  901 CCCATTCNAC CACCAAGCGA AACATCNCAT CNAGCGNNCN CGTACTCNGN NGGAANCCNN
  961 NCTNGNCNAT CNNNATGNNN NGGNNNANNN NCATCNNNGN NNCNCNNNNN NNNNANCTNN
1021 NNNNCNNNNN NNNANNNNNN NNNN
//
Primer: ccdB-C_F
Region: ccdB, NeoR_KanR
Sequence file: RDB18337_B0Gfg.seq check
>D07105A5_B0Gf_2_ccdB-C_F_B03_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN TCNNNNNNNA GCTGGCGCAT GNNGNCCNCC GNNTATGGCN
   61 NNGTGTGCCG GTCTCCGTTA TCGGGGAAGA AGTGGCTGAT CTCAGCCACC GCGAAAATGA
  121 CATCAAAAAC GCCATTAACC TGATGTTCTG GGGAATATAA ATGTCAGGCA TGAGATTATC
  181 AAAAAGGATC TTCACCTAGA TCCTTTTCAC GTAGAAAGCC AGTCCGCAGA AACGGTGCTG
  241 ACCCCGGATG AATGTCAGCT ACTGGGCTAT CTGGACAAGG GAAAACGCAA GCGCAAAGAG
  301 AAAGCAGGTA GCTTGCAGTG GGCTTACATG GCGATAGCTA GACTGGGCGG TTTTATGGAC
  361 AGCAAGCGAA CCGGAATTGC CAGCTGGGGC GCCCTCTGGT AAGGTTGGGA AGCCCTGCAA
  421 AGTAAACTGG ATGGCTTTCT TGCCGCCAAG GATCTGATGG CGCAGGGGAT CAAGCTCTGA
  481 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC
  541 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG
  601 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC
  661 CGACCTGTCC GGTGCCCTGA ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC
  721 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG
  781 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA
  841 GAAAGTATCC ATCATGGCTG ATGCNATGCG GCGGCTGCAT ACGCTTGATC CGGCTACNTG
  901 CCCATTCNAC CACCAAGCGA AACATCNCAT CNAGCGNNCN CGTACTCNGN NGGAANCCNN
  961 NCTNGNCNAT CNNNATGNNN NGGNNNANNN NCATCNNNGN NNCNCNNNNN NNNNANCTNN
1021 NNNNCNNNNN NNNANNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020).

2021.03.05

GNP_filter3_RDBDEP_html_201222.pl