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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAAV-hSyn-OsTIR1(F74A) (#RDB18340)

Adeno-associated virus (AAV) expression vector of OsTIR1(F74A) and mAID-EGFP-NES marker with hSyn promoter.

Clone info. Adeno-associated virus (AAV) expression vector of OsTIR1(F74A) and mAID-EGFP-NES marker with hSyn promoter, see Fig. 2A, B and SFig. 4A of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone pAAV-hSyn (plasmid)
Selectable markers Ampicillin
Gene/insert name Oryza sativa OsTIR1 cDNA Arabidopsis thaliana IAA17 cDNA Aequorea victoria GFP (EGFP) cDNA
Depositor|Developer Kanemaki, Masato |
 
Sequence (full) RDB18340zzk01.seq provided by the depositor

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。

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RDB18340 pAAV-hSyn-OsTIR1(F74A) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAAV-hSyn-OsTIR1(F74A) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18340).

Reference section:

Yesbolatova, A., Saito, Y., Kitamoto, N., Makino-Itou, H., Ajima, R., Nakano, R., Nakaoka, H., Fukui, K., Gamo, K., Tominari, Y., Takeuchi, H., Saga, Y., Hayashi, K., Kanemaki, M.T., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18340_B0Gfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: OsTIR1_R2 (Pr0891)
Region: OsTIR1 5',ccvOsTIR1 codon plus (F74A),hSyn pro
Sequence file: RDB18340_B0Gfa.seq check
>18340_18340_B0Gf_1_OsTIR1-N_R2_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNATTNNCT TCGCCNTTGN NGCNNGCAAN GTTCNNGGNN
   61 NCCANGCTGG TACATGANTC TGGNNANCAA GACAGCCATC TGGGGCCTCT ATCCTCCACT
  121 TCATTCTCCT GGAGGTCCAG CTCCCGCAGC AGCTTACAGT GGGAGGCCAC GGCTGCCAGT
  181 CCATCGGTGC TGAAGCCTTC GCAAGAAATC AGCACCAGTG CGCGAAACCT AGGGAAGCTC
  241 CGAGCCAGCA GCTCCAGACT TTCGTCAGAG ACCACCATTC GCTTCATGCG CAGTTCCTCC
  301 AGTCCATGAC AGCCGCGAGC GGCTGCCTCG ATCCAAGGGC CGGCGTATCC TCCCCAATCA
  361 GGGGGCACCA GGTTAAAGTC GGCGGCGTGG GGTTTCCCCT TGACGGTCAG TGCGCGCACA
  421 TTTGGAAACC TAGCGGCGAC CCTGCCTGCT CTCACAGCAT AGCAGTTGCC GACGAACACT
  481 CCTCTCCGGC TCAGGCGTTC GATCTCGTAC CACACTTTGC AGACCAGGCT CACTGTGTTT
  541 CTATCTCTCT GTGCAGGCAG GAAGCTAAAA ATGTGTTCGA CGACCTCTTC AGGAAAGTAT
  601 GTCATGGTGG CGATCCTCTC GACTGCGCTC TCAGGCACGA CACGACTCCT CCGCTGCCCA
  661 CCGCAGACTG AGGCAGCGCT GAGTCGCCGG CGCCGCAGCG CAGATGGTCG CGCCCGTGCC
  721 CCCCTATCTC GCGCCTCGCG TGGTGCGGTC CGGCTGGGCC GGCGGCGGCG CGNANGCNAN
  781 CAANGTGGCC GGGAAGGNNN NNNTGNGNNG NANNGGCGAG TGANNTCNNC GCGCCNTCAG
841 TGNTGAGGNN NNNNNGNNNN NNNNNN
//
Primer: EGFP-N_R2 (Pr0905)
Region: EGFP 3',NES
Sequence file: RDB18340_B0Gfb.seq check
>18340_18340_B0Gf_1_EGFP-N_R2_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNNANNNNNG CNCGCCGTAN GTCAGGGTGG TCACGAGGGT
   61 GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAAC TTCAGGGTCA GCTTGCCGTA
  121 GGTGGCATCG CCCTCGCCCT CGCCGGACAC GCTGAACTTG TGGCCGTTTA CGTCGCCGTC
  181 CAGCTCGACC AGGATGGGCA CCACCCCGGT GAACAGCTCC TCGCCCTTGC TCACCATGCT
  241 AGCTTTATAC ATCCTCAAAT CGATTTTCCT CAAGTACGGT GCTCCGTCCA TTGATACCTT
  301 CACGAACGCC GCCGCCTCCG GGCCACCGCT TGATTTTTGG CAGGAAACCA TCACGTTCTT
  361 CCGGTATGAT CTCACCGGTG GCCATCCCAC AACTTGTGCC TTGGCCGGAG GTTTGGCTGG
  421 ATCTTTAGGA CAAGCACTCT TCTCCTTAGA TCTAGGTCCA GGGTTCTCCT CCACGTCTCC
  481 AGCCTGCTTC AGCAGGCTGA AGTTAGTAGC TCCGCTTCCT GATCTCAGAA TCTTCACAAA
  541 GTTGGGAGCA TCATCTCTTG CCCCTGCGGT GGTCCGATAC ACATACAGTT TCTCCACCTT
  601 TGGCAGGTCG CCATGATTTT CCTCCATTTC GTTAGACCCG TCCCGCTCAT TAATGACTTC
  661 CACGTTCAGC ATAGGCATTT TACTAGCCAG GACCTGACAG CCCTTCAGAG TCACATTGCA
  721 ACTAGACATC CACAGGCTCC TCATTGTCTC GTATCTGGCA AAGTTTCCCA GCAGANCGGC
  781 ATCCCCGAAA GGGCTGTCCC GGATTTCCAG TTTTCGCAGA NTCTTACACC CGTTCATCAC
  841 GTGCATCATG CCCTTATCGG ANTCTCCGGC AAATGCAATG CTCAGCATCT CCAGCTGCTN
  901 ANCATACNNG CCNANGTACA TGAACACTTT GTCTGTCAGC NNNNCNGAAN NGCTCANCCN
961 NNNNANNNCC NNNNNNNNNN
//
Primer: EGFP-N_rev (Pr0156)
Region: EGFP 5',mini-AID,P2A,OsTIR1 3'
Sequence file: RDB18340_B0Gfc.seq check
>18340_18340_B0Gf_1_EGFP-N_rev_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNGN NNNNNNNNGN CGANGGCGNN GCCNCCTACG GCAAGCTGAN
   61 CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC
  121 CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT
  181 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA
  241 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT
  301 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA
  361 CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT
  421 GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA
  481 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC
  541 CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT
  601 CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGGGTG CAGGCGCCCA
  661 AGCTTCGAAT TCTGCAGTCG ACCTGCCCCC CCTGGAGCGC CTGACCCTGA TCGACGGTAC
  721 CGCGGGCCCG GGATCCACCG GATCTAGATA ACTGATCATA ATCAGCCATA CCACATTTGT
  781 ANAGGTTTTA CTTGCTTNAA AAAACCTCCC ACACCTCCCN CTGANCCTGA NACATAAAAT
  841 GAATGCAATT GNTGNTGTTA ACTTGTTTAT TGCANCTTAT AATGGTTACA AATAAAGCAA
  901 TAGCATCNCA AATTTCANNA ANNNAANNCN TTTTTTTCNN TGNNTTCTAN NTNGTGGTTT
  961 GNCCNAANTC ATCANNGNAT CNTNNNNNNN NATATCNANN NNTANCNANN NTCNNNCNNN
1021 NNNNNTNNNN NAANNNNNNN ANNNNNNNNN N
//
Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',NES,SV40 pA,WPRE
Sequence file: RDB18340_B0Gfd.seq check
>18340_18340_B0Gf_1_EGFP-C2_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNCNCNTGGT CCTGCTGGAN TTCGTGANCG CCGCCGGGNT
   61 CACTCTCGGC ATGGACGAGC TGTACAAGGG TGCAGGCGCC CAAGCTTCGA ATTCTGCAGT
  121 CGACCTGCCC CCCCTGGAGC GCCTGACCCT GATCGACGGT ACCGCGGGCC CGGGATCCAC
  181 CGGATCTAGA TAACTGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT
  241 AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT
  301 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC
  361 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC
  421 TTAAATTCGA TATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT
  481 TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC
  541 CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT
  601 GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA
  661 CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT
  721 CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG
  781 CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGNCGGGGA
  841 ANTCATCGTC CTTTCCTTGN CTGCTCGCCT ATGTTGCNAC CTGNATTCTG CGCGGGNNGN
  901 CCTTCTGCTA NGTCCCTTCG GNCCTNNANT NCANCGGACC TTNCTNNCCN GCGGNCTGNT
  961 GNCGGCTCTG CGGNNNNTTN CNNNNNTNCN CNTTNNNCNN CANANNANNN NNNANNNNCC
1021 NTTNNNNNNN NCNNCCCNNN NNNNNNN
//
Primer: SV40_F2 (Pr1194)
Region: WPRE,bGH pA,AAV2 ITR
Sequence file: RDB18340_B0Gfe.seq check
>18340_18340_B0Gf_1_SV40_F2_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC
   61 CCTTTGGGCC GCCTCCCCGC ATCGATACCG AGCGCTGCTC GAGAGATCTA CGGGTGGCAT
  121 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC
  181 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT
  241 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG
  301 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA
  361 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC
  421 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC
  481 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC
  541 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTGTAG
  601 GTAACCACGT GCGGACCGAG CGGCCGCAGG AACCCCTAGT GATGGAGTTG GCCACTCCCT
661 CTCTGCGCGC TCNCTCNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]