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pMK394 (mAID-Nluc-Neo) (#RDB16822)

A plasmid for construction of C-terminal mAID-Nluc-Neo tagging donors.

Clone info. A plasmid for construction of C-terminal mAID-Nluc-Neo tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Oplophorus gracilirostris luciferase cDNA
Depositor|Developer Kanemaki, Masato |
 

Distribution information

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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
MTAに書く付加的使用条件:
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16822 pMK394 (mAID-Nluc-Neo) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK394 (mAID-Nluc-Neo) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16822).

Reference section:

Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16822_A8Lpp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310)
Region: T7 pro,insert 5'(Linker,mAID,Nluc 5')
Sequence file: RDB16822_A8Lpd.seq check
>D06157B9_A8Lp_1_pUC-M13_-100_A08_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGGNNNGCC NGGNTTTTCC CNGTCACGNC GTTGTAAAAC
   61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGAGC TCCCCGGGAT
  121 CCGGTGCAGG CGCCAAGGAG AAGAGTGCTT GTCCTAAAGA TCCAGCCAAA CCTCCGGCCA
  181 AGGCACAAGT TGTGGGATGG CCACCGGTGA GATCATACCG GAAGAACGTG ATGGTTTCCT
  241 GCCAAAAATC AAGCGGTGGC CCGGAGGCGG CGGCGTTCGT GAAGGTATCA ATGGACGGAG
  301 CACCGTACTT GAGGAAAATC GATTTGAGGA TGTATAAAGC TAGCATGGTC TTCACACTCG
  361 AAGATTTCGT TGGGGACTGG CGACAGACAG CCGGCTACAA CCTGGACCAA GTCCTTGAAC
  421 AGGGAGGTGT GTCCAGTTTG TTTCAGAATC TCGGGGTGTC CGTAACTCCG ATCCAAAGGA
  481 TTGTCCTGAG CGGTGAAAAT GGGCTGAAGA TCGACATCCA TGTCATCATC CCGTATGAAG
  541 GTCTGAGCGG CGACCAAATG GGCCAGATCG AAAAAATTTT TAAGGTGGTG TACCCTGTGG
  601 ATGATCATCA CTTTAAGGTG ATCCTGCACT ATGGCACACT GGTAATCGAC GGGGTTACGC
  661 CGAACATGAT CGACTATTTC GGACGGCCGT ATGAAGGCAT CGCCGTGTTC GACNGCAAAA
  721 AGATCACTGT AACAGGGACC CTGTGGAACG GCAACAAAAT TATCGACGAN CGCCTGNTCA
  781 ACCCCGACNG CTCCCTGCTN TTCCGANTAA CCATCAACGG NNTGANCGGC TGGCGGCTGN
  841 GCGAACGCNT TCTGGCGNNA NCGGCCGCTC NANTCNAGAT AACTGATCAT ANTCAGCCNN
901 ACCNCATTTN GTANNNGTTT TNNNTNNCNT NNAAANNNCN TCCNNNNNCN NNNNNNNNN
//
Primer: SV40pA3element Reverse (Pr0108)
Region: T3 pro,insert 3'(TK pA,NeoR KanR)
Sequence file: RDB16822_A8Lpe.seq check
>16822_16822_B3C8F_SV40pA3'element Reverse_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNGNNNNNN NNNNATGNNN NNNNCCNGCA ACGCGGCCTT TTTACGGTTC
   61 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG
  121 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG
  181 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC
  241 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC
  301 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC
  361 TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA
  421 AACAGCTATG ACCATGATTA CGCCAAGCGC GCAATTAACC CTCACTAAAG GGAACAAAAG
  481 CTGGGTACCG GATCCAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC
  541 ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG
  601 TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG
  661 AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT
  721 GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT
  781 CGTCAAGAAG GCGANAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA
  841 CGAGGAANCG GTCAGCCCAT TCNCCGCCAA GCTCTNCANC NATATCACGG GTAGCNAACG
  901 CNATGTCCTG ATAGCNGNCC GCCNCACCNA NCCNGNCCNC AGTCGANNNA NTNCNGAAAA
961 NCNGNNNNTT TTNCNCCNNG ANNNTNNNNN NNNNN
//
Primer: SV40pro_R2 (Pr0516)
Region: insert mid(SV40 pro_ori,AmpR pro,
Sequence file: RDB16822_A8Lpf.seq check
>D06157B9_A8Lp_1_SV40pro_R2_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNCTGCCTGC TGGGGANCCT GGGGNNTTTC CNCACCCTAA
   61 CTGACACACA TTCNNCAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT
  121 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA
  181 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT
  241 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC
  301 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC
  361 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG
  421 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA
  481 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC
  541 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG
  601 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT
  661 AACAAAATAT TAACGCTTAC AATTTACGCG TTAAGATACA TTGATGAGTT TGGACAAACC
  721 ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA
  781 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAANN ACNATTGCAT TCATTTTATG
  841 TTTCNNGTTC AGGGGGANGT GTGGGAGNNT TTTTTAAAGC AAGTAAAANC TCTACAAATG
  901 TGNNATGGNT GATTATGANC AGTTATCTNG ACTCGAGCNG NNNGCTTANN CCAGANTGNG
961 TTCGCNCANC CGCCAGNNNN GNCACTCCNN TNNANNGNNN NTNNGNNNNN NNNNN
//
Primer: SV40pro_F (Pr0110)
Region: insert mid(SV40 pro_ori, NeoR_KanR 5')
Sequence file: RDB16822_A8Lpg.seq check
>D06157B9_A8Lp_1_SV40pro_F_C08_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN GNNGNTTTTT TGGANGCCTA GGCTTTTGCA
   61 AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC
  121 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA
  181 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT
  241 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG ACGAGGCAGC GCGGCTATCG
  301 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA
  361 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT
  421 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG
  481 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG
  541 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC
  601 GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT
  661 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC
  721 TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAN CGTTGGCTAC CCGTGATATT
  781 GCTGAANAGC TTGNNGGCGA ATGGNCTGAN CGCTTCCTCG TGCTTTACNN TATCGCCGCT
  841 CCCGANTCGC ANCGCATCGC CTTCTATCGC NNTCTTGANG ANTTCNTTCT GAGCGGGANN
  901 CTNGGGTNCN AAATGANCGA NNNANCGACN NCCNANNNNG NNNTCANNAG ATTTNNANTN
961 NNNNGNCNGC CNTNCTNNNN AANNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]