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pMK394 (mAID-Nluc-Neo)

A plasmid for construction of C-terminal mAID-Nluc-Neo tagging donors.

Catalog number RDB16822
Resource name pMK394 (mAID-Nluc-Neo)
Clone info. A plasmid for construction of C-terminal mAID-Nluc-Neo tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Oplophorus gracilirostris luciferase cDNA
Depositor Kanemaki, Masato |
 
Other clones in our bank Arabidopsis thaliana AXR3 (KEGG orthology K14484) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16822 pMK394 (mAID-Nluc-Neo) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16822_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 RDB16822_A8Lpa.seq
>D06157B9_A8Lp_1_pUC-M13_-100_A08_02_ABI24.ab1
    1 GGTGGGTTAA GCGATTAAGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC
   61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGAGC TCCCCGGGAT
  121 CCGGTGCAGG CGCCAAGGAG AAGAGTGCTT GTCCTAAAGA TCCAGCCAAA CCTCCGGCCA
  181 AGGCACAAGT TGTGGGATGG CCACCGGTGA GATCATACCG GAAGAACGTG ATGGTTTCCT
  241 GCCAAAAATC AAGCGGTGGC CCGGAGGCGG CGGCGTTCGT GAAGGTATCA ATGGACGGAG
  301 CACCGTACTT GAGGAAAATC GATTTGAGGA TGTATAAAGC TAGCATGGTC TTCACACTCG
  361 AAGATTTCGT TGGGGACTGG CGACAGACAG CCGGCTACAA CCTGGACCAA GTCCTTGAAC
  421 AGGGAGGTGT GTCCAGTTTG TTTCAGAATC TCGGGGTGTC CGTAACTCCG ATCCAAAGGA
  481 TTGTCCTGAG CGGTGAAAAT GGGCTGAAGA TCGACATCCA TGTCATCATC CCGTATGAAG
  541 GTCTGAGCGG CGACCAAATG GGCCAGATCG AAAAAATTTT TAAGGTGGTG TACCCTGTGG
  601 ATGATCATCA CTTTAAGGTG ATCCTGCACT ATGGCACACT GGTAATCGAC GGGGTTACGC
  661 CGAACATGAT CGACTATTTC GGACGGCCGT ATGAAGGCAT CGCCGTGTTC GACGGCAAAA
  721 AGATCACTGT AACAGGGACC CTGTGGAACG GCAACAAAAT TATCGACGAG CGCCTGATCA
  781 ACCCCGACGG CTCCCTGCTG TTCCGAGTAA CCATCAACGG AGTGACCGGC TGGCGGCTGT
  841 GCGAACGCAT TCTGGCGTAA GCGGCCGCTC GAGTCTAGAT AACTGATCAT AATCAGCCAT
  901 ACCACATTTT GTAGAGGTTT TTACTTGCTT TAAAAAACCC TCCCACACCC TCCCCCTGAA
  961 CCCTGAAACA TAAAATGAAA TGCAAATTGT TTGTTGTTAA ACTTTGTTTA TTTGCAGCCT
 1021 AATATGGTTA CAAATAAGCC AATTAGCCAT CACAATTTCC ACAATAAGGC ATTTTTTTTC
1081 ACTGCCAATT CTAGTTGAGT GGGACTG
//
Primer: SV40pro_R2 RDB16822_A8Lpb.seq
>D06157B9_A8Lp_1_SV40pro_R2_F01_06_ABI08.ab1
    1 TAGTCCATGC TTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA
   61 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT
  121 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA
  181 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT
  241 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC
  301 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC
  361 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG
  421 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA
  481 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC
  541 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG
  601 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT
  661 AACAAAATAT TAACGCTTAC AATTTACGCG TTAAGATACA TTGATGAGTT TGGACAAACC
  721 ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA
  781 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG
  841 TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTTAAAGC AAGTAAAACC TCTACAAATG
  901 TGGTATGGCT GATTATGATC AGTTATCTAG ACTCGAGCGG GCCGCTTACG CCAGAATGCG
  961 TTCGCACAGC CGCCAGCCGG GTCACTCCGT TTGATGGTTA CTCGGGAACA GCAAGGGAGC
 1021 CGTCGGGGTT GATCAGGCGC TCGTCGATAA TTTTTGTTGC GTTCACAGGG TTCCCTTGTT
 1081 ACAGTGATTC TTTTTGCCGT CGAACACGCG AATGGCTCAT ACGCCGTTCG AAAATAGTCG
1141 AATCAATGGT TTCGGGCGGG TAAATCTCG
//
Primer: SV40pro_F RDB16822_A8Lpc.seq
>D06157B9_A8Lp_1_SV40pro_F_C08_08_ABI24.ab1
    1 CGTGTCGGGG TTAGCATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC
  121 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA
  181 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT
  241 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG ACGAGGCAGC GCGGCTATCG
  301 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA
  361 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT
  421 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG
  481 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG
  541 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC
  601 GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT
  661 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC
  721 TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT
  781 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT
  841 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTTCT GAGCGGGACT
  901 CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAAACCTG CCATCACGAG ATTTCGATTC
  961 CACCGCCCGC CTTTCTATGA AAAGGTTGGC TTTCGGAAAC GATTTTCCCG GAACGCCGGC
 1021 TGGAATGATC CTCCCAGCGG CGGGATCTCA ATGCCTGATT CTCGCCCACC CTTAGGGGAA
1081 GGCCTTACCT GAATACACCG AATGGAGAAA C
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

2019.07.12

GNP_filter3_RDBDEP_html_190431.pl