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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK394 (mAID-Nluc-Neo)

A plasmid for construction of C-terminal mAID-Nluc-Neo tagging donors.

Catalog number RDB16822
Resource name pMK394 (mAID-Nluc-Neo)
Clone info. A plasmid for construction of C-terminal mAID-Nluc-Neo tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Oplophorus gracilirostris luciferase cDNA
Depositor|Developer Kanemaki, Masato |
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External Database
Arabidopsis thaliana AtIAA17 Oplophorus gracilirostris luciferase

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16822 pMK394 (mAID-Nluc-Neo) DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16822_A8Lpp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-M13_-100
          Sequence file: RDB16822_A8Lpa.seq check
          >D06157B9_A8Lp_1_pUC-M13_-100_A08_02_ABI24.ab1
              1 GGTGGGTTAA GCGATTAAGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC
             61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGAGC TCCCCGGGAT
            121 CCGGTGCAGG CGCCAAGGAG AAGAGTGCTT GTCCTAAAGA TCCAGCCAAA CCTCCGGCCA
            181 AGGCACAAGT TGTGGGATGG CCACCGGTGA GATCATACCG GAAGAACGTG ATGGTTTCCT
            241 GCCAAAAATC AAGCGGTGGC CCGGAGGCGG CGGCGTTCGT GAAGGTATCA ATGGACGGAG
            301 CACCGTACTT GAGGAAAATC GATTTGAGGA TGTATAAAGC TAGCATGGTC TTCACACTCG
            361 AAGATTTCGT TGGGGACTGG CGACAGACAG CCGGCTACAA CCTGGACCAA GTCCTTGAAC
            421 AGGGAGGTGT GTCCAGTTTG TTTCAGAATC TCGGGGTGTC CGTAACTCCG ATCCAAAGGA
            481 TTGTCCTGAG CGGTGAAAAT GGGCTGAAGA TCGACATCCA TGTCATCATC CCGTATGAAG
            541 GTCTGAGCGG CGACCAAATG GGCCAGATCG AAAAAATTTT TAAGGTGGTG TACCCTGTGG
            601 ATGATCATCA CTTTAAGGTG ATCCTGCACT ATGGCACACT GGTAATCGAC GGGGTTACGC
            661 CGAACATGAT CGACTATTTC GGACGGCCGT ATGAAGGCAT CGCCGTGTTC GACGGCAAAA
            721 AGATCACTGT AACAGGGACC CTGTGGAACG GCAACAAAAT TATCGACGAG CGCCTGATCA
            781 ACCCCGACGG CTCCCTGCTG TTCCGAGTAA CCATCAACGG AGTGACCGGC TGGCGGCTGT
            841 GCGAACGCAT TCTGGCGTAA GCGGCCGCTC GAGTCTAGAT AACTGATCAT AATCAGCCAT
            901 ACCACATTTT GTAGAGGTTT TTACTTGCTT TAAAAAACCC TCCCACACCC TCCCCCTGAA
            961 CCCTGAAACA TAAAATGAAA TGCAAATTGT TTGTTGTTAA ACTTTGTTTA TTTGCAGCCT
           1021 AATATGGTTA CAAATAAGCC AATTAGCCAT CACAATTTCC ACAATAAGGC ATTTTTTTTC
          1081 ACTGCCAATT CTAGTTGAGT GGGACTG
          //
          Primer: SV40pro_R2
          Sequence file: RDB16822_A8Lpb.seq check
          >D06157B9_A8Lp_1_SV40pro_R2_F01_06_ABI08.ab1
              1 TAGTCCATGC TTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA
             61 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT
            121 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA
            181 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT
            241 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC
            301 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC
            361 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG
            421 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA
            481 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC
            541 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG
            601 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT
            661 AACAAAATAT TAACGCTTAC AATTTACGCG TTAAGATACA TTGATGAGTT TGGACAAACC
            721 ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA
            781 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG
            841 TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTTAAAGC AAGTAAAACC TCTACAAATG
            901 TGGTATGGCT GATTATGATC AGTTATCTAG ACTCGAGCGG GCCGCTTACG CCAGAATGCG
            961 TTCGCACAGC CGCCAGCCGG GTCACTCCGT TTGATGGTTA CTCGGGAACA GCAAGGGAGC
           1021 CGTCGGGGTT GATCAGGCGC TCGTCGATAA TTTTTGTTGC GTTCACAGGG TTCCCTTGTT
           1081 ACAGTGATTC TTTTTGCCGT CGAACACGCG AATGGCTCAT ACGCCGTTCG AAAATAGTCG
          1141 AATCAATGGT TTCGGGCGGG TAAATCTCG
          //
          Primer: SV40pro_F
          Sequence file: RDB16822_A8Lpc.seq check
          >D06157B9_A8Lp_1_SV40pro_F_C08_08_ABI24.ab1
              1 CGTGTCGGGG TTAGCATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
             61 AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC
            121 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA
            181 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT
            241 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG ACGAGGCAGC GCGGCTATCG
            301 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA
            361 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT
            421 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG
            481 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG
            541 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC
            601 GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT
            661 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC
            721 TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT
            781 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT
            841 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTTCT GAGCGGGACT
            901 CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAAACCTG CCATCACGAG ATTTCGATTC
            961 CACCGCCCGC CTTTCTATGA AAAGGTTGGC TTTCGGAAAC GATTTTCCCG GAACGCCGGC
           1021 TGGAATGATC CTCCCAGCGG CGGGATCTCA ATGCCTGATT CTCGCCCACC CTTAGGGGAA
          1081 GGCCTTACCT GAATACACCG AATGGAGAAA C
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591.

          2021.11.02

          GNP_filter3_RDBDEP_html_211029.pl