A plasmid for construction of C-terminal mAID-Nluc-Neo tagging donors.
Clone info. | A plasmid for construction of C-terminal mAID-Nluc-Neo tagging donors. |
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Vector backbone | pBluescript (plasmid) |
Size of vector backbone | 2.9 kb |
Selectable markers | Am^r (E. coli) |
Gene/insert name | Arabidopsis thaliana AtIAA17 cDNA Oplophorus gracilirostris luciferase cDNA |
Depositor|Developer | Kanemaki, Masato | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() MTA, for use for for-profit purpose [Word ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 提供同意書 (MTA, 営利目的用)[Word ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 MTAに書く付加的使用条件: 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB16822 | pMK394 (mAID-Nluc-Neo) | DNA solution |
Materials & Methods section:
The pMK394 (mAID-Nluc-Neo) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16822). |
Reference section:
Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB16822_A8Lpp1-2.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pUC-M13_-100 (Pr0310) Region: T7 pro,insert 5'(Linker,mAID,Nluc 5') Sequence file: RDB16822_A8Lpd.seq ![]() |
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>D06157B9_A8Lp_1_pUC-M13_-100_A08_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NGGGNNNGCC NGGNTTTTCC CNGTCACGNC GTTGTAAAAC 61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGAGC TCCCCGGGAT 121 CCGGTGCAGG CGCCAAGGAG AAGAGTGCTT GTCCTAAAGA TCCAGCCAAA CCTCCGGCCA 181 AGGCACAAGT TGTGGGATGG CCACCGGTGA GATCATACCG GAAGAACGTG ATGGTTTCCT 241 GCCAAAAATC AAGCGGTGGC CCGGAGGCGG CGGCGTTCGT GAAGGTATCA ATGGACGGAG 301 CACCGTACTT GAGGAAAATC GATTTGAGGA TGTATAAAGC TAGCATGGTC TTCACACTCG 361 AAGATTTCGT TGGGGACTGG CGACAGACAG CCGGCTACAA CCTGGACCAA GTCCTTGAAC 421 AGGGAGGTGT GTCCAGTTTG TTTCAGAATC TCGGGGTGTC CGTAACTCCG ATCCAAAGGA 481 TTGTCCTGAG CGGTGAAAAT GGGCTGAAGA TCGACATCCA TGTCATCATC CCGTATGAAG 541 GTCTGAGCGG CGACCAAATG GGCCAGATCG AAAAAATTTT TAAGGTGGTG TACCCTGTGG 601 ATGATCATCA CTTTAAGGTG ATCCTGCACT ATGGCACACT GGTAATCGAC GGGGTTACGC 661 CGAACATGAT CGACTATTTC GGACGGCCGT ATGAAGGCAT CGCCGTGTTC GACNGCAAAA 721 AGATCACTGT AACAGGGACC CTGTGGAACG GCAACAAAAT TATCGACGAN CGCCTGNTCA 781 ACCCCGACNG CTCCCTGCTN TTCCGANTAA CCATCAACGG NNTGANCGGC TGGCGGCTGN 841 GCGAACGCNT TCTGGCGNNA NCGGCCGCTC NANTCNAGAT AACTGATCAT ANTCAGCCNN 901 ACCNCATTTN GTANNNGTTT TNNNTNNCNT NNAAANNNCN TCCNNNNNCN NNNNNNNNN // |
Primer: SV40pA3element Reverse (Pr0108) Region: T3 pro,insert 3'(TK pA,NeoR KanR) Sequence file: RDB16822_A8Lpe.seq ![]() |
>16822_16822_B3C8F_SV40pA3'element Reverse_G01_07_ABI08.ab1 1 NNNNNNNNNN NNNGNNNNNN NNNNATGNNN NNNNCCNGCA ACGCGGCCTT TTTACGGTTC 61 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG 121 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG 181 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC 241 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC 301 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC 361 TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA 421 AACAGCTATG ACCATGATTA CGCCAAGCGC GCAATTAACC CTCACTAAAG GGAACAAAAG 481 CTGGGTACCG GATCCAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC 541 ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG 601 TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG 661 AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT 721 GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT 781 CGTCAAGAAG GCGANAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA 841 CGAGGAANCG GTCAGCCCAT TCNCCGCCAA GCTCTNCANC NATATCACGG GTAGCNAACG 901 CNATGTCCTG ATAGCNGNCC GCCNCACCNA NCCNGNCCNC AGTCGANNNA NTNCNGAAAA 961 NCNGNNNNTT TTNCNCCNNG ANNNTNNNNN NNNNN // |
Primer: SV40pro_R2 (Pr0516) Region: insert mid(SV40 pro_ori,AmpR pro, Sequence file: RDB16822_A8Lpf.seq ![]() |
>D06157B9_A8Lp_1_SV40pro_R2_F01_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNCTGCCTGC TGGGGANCCT GGGGNNTTTC CNCACCCTAA 61 CTGACACACA TTCNNCAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 121 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 181 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 241 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 301 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 361 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 421 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 481 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 541 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 601 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 661 AACAAAATAT TAACGCTTAC AATTTACGCG TTAAGATACA TTGATGAGTT TGGACAAACC 721 ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA 781 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAANN ACNATTGCAT TCATTTTATG 841 TTTCNNGTTC AGGGGGANGT GTGGGAGNNT TTTTTAAAGC AAGTAAAANC TCTACAAATG 901 TGNNATGGNT GATTATGANC AGTTATCTNG ACTCGAGCNG NNNGCTTANN CCAGANTGNG 961 TTCGCNCANC CGCCAGNNNN GNCACTCCNN TNNANNGNNN NTNNGNNNNN NNNNN // |
Primer: SV40pro_F (Pr0110) Region: insert mid(SV40 pro_ori, NeoR_KanR 5') Sequence file: RDB16822_A8Lpg.seq ![]() |
>D06157B9_A8Lp_1_SV40pro_F_C08_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN GNNGNTTTTT TGGANGCCTA GGCTTTTGCA 61 AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC 121 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA 181 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT 241 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG ACGAGGCAGC GCGGCTATCG 301 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA 361 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 421 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG 481 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG 541 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC 601 GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT 661 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 721 TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAN CGTTGGCTAC CCGTGATATT 781 GCTGAANAGC TTGNNGGCGA ATGGNCTGAN CGCTTCCTCG TGCTTTACNN TATCGCCGCT 841 CCCGANTCGC ANCGCATCGC CTTCTATCGC NNTCTTGANG ANTTCNTTCT GAGCGGGANN 901 CTNGGGTNCN AAATGANCGA NNNANCGACN NCCNANNNNG NNNTCANNAG ATTTNNANTN 961 NNNNGNCNGC CNTNCTNNNN AANNNNNNNN NN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP] |
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