RDB13934 - pMK291 (mAID-mClover-Bsr)

Resource data sheet
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pMK291 (mAID-mClover-Bsr)

A plasmid for construction of C-terminal mAID-mClover tagging donors.


Catalog number	RDB13934
Resource name	pMK291 (mAID-mClover-Bsr)
Alternative name	mAID-mClover-Bsr, 1024
Clone info.	A plasmid for construction of C-terminal mAID-mClover tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone. To make 0.5 M stock solution, dissolve indole-3-acetic acid (IAA) (Sigma-Aldrich, 45533) or 1-naphthaleneacetic acid (NAA) (Sigma-Aldrich, 35745) in 100 % DMSO. IAA solution can be made in 100 % ethanol. Aliquots can be kept for 6 months in the dark at -20oC (Devrekanli, A., Kanemaki, M.T. Methods in Molecular Biology, 1369, 257-278, 2016).
Vector backbone	pBluescriptII KS- (Plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Am^r
Gene/insert name	Arabidopsis thaliana AtIAA17 cDNA Aequorea victoria GFP cDNA
Depositor	Kanemaki, Masato \|
Other clones in our bank	Arabidopsis thaliana AtIAA17 (KEGG orthology K14484) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

Ordering Please visit Information of Request for Distribution.
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]

Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	((Additional form)) GFP-A Please use Schedule A (GFP-A) additionally.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	【追加提供依頼書】GFP-A Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13934	pMK291 (mAID-mClover-Bsr)	DNA solution

cDNA Request [in Japanese] [in English]

References and tips

Electronic file

Featured content	Auxin Inducible Degron (AID) System (English text)
Featured content	Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB13934_A6B8p1_v2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40	RDB13934_A6B8a_2.seq
>D03070A18_A6B8_1_M13(-40)_C05_08.ab1 1 AGGCCGGGGA CGCGCGTATA CGACTCACTA TAGGGCGAAT TGGAGCTCCC CGGGATCCGG 61 TGCAGGCGCC AAGGAGAAGA GTGCTTGTCC TAAAGATCCA GCCAAACCTC CGGCCAAGGC 121 ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG AACGTGATGG TTTCCTGCCA 181 AAAATCAAGC GGTGGCCCGG AGGCGGCGGC GTTCGTGAAG GTATCAATGG ACGGAGCACC 241 GTACTTGAGG AAAATCGATT TGAGGATGTA TAAAGCTAGC ATGGTGAGCA AGGGCGAGGA 301 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA 361 GTTCAGCGTC CGCGGCGAGG GCGAGGGCGA TGCCACCAAC GGCAAGCTGA CCCTGAAGTT 421 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCTTCGGCTA 481 CGGCGTGGCC TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC 541 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTCT TTCAAGGACG ACGGTACCTA 601 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA 661 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTTCAA 721 CAGCCACAAC GTCTATATCA CGGCCGACAA GCAGAAGAAC GGCATCAAGG CTAACTTCAA 781 GATCCGCCAC AACGTTGAGG ACGGCAGCGT GCAGCTCGCC GGACCACTAC CAGCAGAACA 841 CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC CATCAGTCCA 901 AGCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGGA GTTCGTGACC 961 GCCGCCGGGG ATTACACATG GCATGGACGA GCTGTACAAG TAATCTAGAT AACTGATCAT 1021 ATCAGCCATA CCACATTTGT AGGAGGTTTT ACTGGCTTAA AAACCTCCCA CACTCCCCCT 1081 TGACCTGAAC ATTAATGATG CCATTGGATG TTGGTACTTG GTTTATTGCC AGCCTTATAT 1141 AATGTGGCT //
Primer: PGK_N_rev	RDB13934_A6B8b_2.seq
>D03070A18_A6B8_1_PGK-N-rev_F06_18.ab1 1 CATGGGACGC CTCCCCTACC CCGGTAGAAT TTCGAGGTCG AGAATCCTAA GCTTGGCTGG 61 ACGTAAACTC CTCTTCAGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATATT 121 AAGGGTTATT GAATATGATC GACGCGTTAA GATACATTGA TGAGTTTGGA CAAACCACAA 181 CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG 241 TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC 301 AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA 361 TGGCTGATTA TGATCAGTTA TCTAGATTAC TTGTACAGCT CGTCCATGCC ATGTGTAATC 421 CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG 481 CTCAGCTTGG ACTGATGGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCG 541 ATGGGGGTGT TCTGCTGGTA GT //
Primer: PGKpro_F	RDB13934_A6B8c_2.seq
>D03070A18_A6B8_1_PGK-proF_A08_02.ab1 1 AGTCGGCGAT CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCATCCATCT AGATCTCGAG 61 CAGCTGAAGC TTACCGCTAG CATGAAAACA TTTAACATTT CTCAACAAGA TCTAGAATTA 121 GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG ATAATAAACA TCATGTGGGA 181 GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG TACATATTGA AGCGTATATA 241 GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA GTGCAGTTTC GAATGGACAA 301 AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT CTGACGAAGT AGATAGAAGT 361 ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA TTTCAGACTA TGCACCAGAT 421 TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA CTACGATTGA AGAACTCATT 481 CCACTCAAAT ATACCCGAAA TTAAGCTAGC CGCCCGCCCC ACGACCCGCA GCGCCCGACC 541 GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT 601 GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA 661 AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG ATTGGAGCTA CGGGGGTGGG 721 GGTGGGGTGG GATTAGATAA ATGCCTGCTC TTTACTGAAG GCTCTTTACT ATTGCTTTAT 781 GATAATGTTT CATAGTTGGA TATCATAATT TAAACAAGCA AAACCAAATT AAGGGCCAGC 841 TCATTCCCTC CCACTCATGA TCTATAGATC TATAGATCTC TCGTGGGATC ATTGTTTTTC 901 TCTTGATTCC CACTTTGTGG TTCTAAGTAC TGTGGGTTTC CAATGTGTCA GTTTCATAGC 961 CTGAAAGAAC GAGATCAGCA AGCCTCTGTT CCCACATACA CTTTCATTTC TCAGTAATTG 1021 TTTGCCAAGG TTCTAAATTT CCATCCAGAG GCTGGTCGAG AATCCGGAAC CCCTTCAATA 1081 TACTTCGTAA TAATGTATGG CCTATACCGA GTATAGTCTC GAAGAAGGTT CAACTAGGAT 1141 TCCAGTTAAC CACCAGCCGT TG //
Primer: M13R	RDB13934_A6B8d_2.seq
>D03070A18_A6B8_1_M13R_C04_07.ab1 1 CCCCGGGCAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGG TACCGGATCC TAGTGAACCT 61 CTTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAT TAAGGGTTCC 121 GGATCTCGAC CAGCTTCTGA TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG 181 TATGTGGAAC AGAGGCTGCT GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA 241 CCACAGTACT TAGAACCACA AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT 301 CTATAGATCT ATAGATCATG AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG 361 TTTAAATTAT GATATCCAAC TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG 421 TAAAGAGCAG GCATTTATCT AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC 481 ATTCAAAATG TAGGTACTCT GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC 541 CATTTTAGTG GACATCTTTA TTGTTTAATA GATCATCAAT TTCTGCATCC CGGGGATCTG 601 ATATCATCGA TGCATGGGGT CGTGCGCTCC TTTCGGTCGG GCGCTGCGGG TCGTGGGGCG 661 GGCGGCTAGC TTAATTTCGG GTATATTTGA GTGGAATGAG TTCTTCAATC GTAGTTTTGA 721 CTAACTTGCC ATTCATTTCT ATTAACACAA AACAATCTGG TGCATAGTCT GAAATCAACT 781 CCCTACACAT ACCACAAGGA CTTACCACTC GAATACTTCT ATCTACTTCG TCAGAATAAG 841 GGTGTCTAAC AGCTACAATC GTGTCAAAAT CCTTTTGTCC ATTCGAAACT GCACTACCAA 901 TCGCAAATGG CTTTCTGCAC AAACAGTTAC TCGTCCCTAT ATACGCTTTC AATATGTACT 961 GCCCGAAAAT GATTTTCTCC TGTTTTCGTA CGAAATTTGC CGCCTCCCCA CCATGAATGT 1021 TTAATTATCC TCATAAAGGC ATTGTAATTC TTTCTCTGTC GCCTACTTTC TACTAAATTT 1081 CTAGAATCTG TGGAGAAATT GTTTAAAATG GATTTTCATG CCTAGCGGTA AGCCTTCAGC 1141 CTGACTCGAA GGAATCTCTA GAAAATTGGA GTTGTGCA //

Please visit Sequencing and PCR primers for primer information.

References

original	Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.
reference	Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318.
reference	Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302 .

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl