Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK291 (mAID-mClover-Bsr)

A plasmid for construction of C-terminal mAID-mClover tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

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Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13934_A6B8p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410) Region: insert mid(Linker,mAID,mClover 5') Sequence file: RDB13934_A6B8e.seq
>D03070A18_A6B8_1_M13(-40)_C05_08.ab1     1 NNNNNNNNNN NNCNCNNANN NNNNTNNCNA TAGGGCGAAT TGGAGCTCCC CGGGNTCCGG    61 TGCAGGCGCC AAGGAGAAGA GTGCTTGTCC TAAAGATCCA GCCAAACCTC CGGCCAAGGC   121 ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG AACGTGATGG TTTCCTGCCA   181 AAAATCAAGC GGTGGCCCGG AGGCGGCGGC GTTCGTGAAG GTATCAATGG ACGGAGCACC   241 GTACTTGAGG AAAATCGATT TGAGGATGTA TAAAGCTAGC ATGGTGAGCA AGGGCGAGGA   301 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA   361 GTTCAGCGTC CGCGGCGAGG GCGAGGGCGA TGCCACCAAC GGCAAGCTGA CCCTGAAGTT   421 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCTTCGGCTA   481 CGGCGTGGCC TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC   541 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTCT TTCAAGGACG ACGGTACCTA   601 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA   661 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTTCAA   721 CAGCCACAAC GTCTATATCA CGGCCGACAA GCAGAAGANC GGCATCAAGG CTAACTTCAA   781 GATCCGCCAC AACGTTGAGG ACGGCAGCGT GCAGCTCGCC NNACCACTAC CANCNNAACA   841 CCCCCATCGG CGANNGNCCC GNNCTGCTGC CCGANANCCA CTACCTGAGC CATCAGTNCA   901 AGCTGANCNA AGANCCCNNC NANAAGCNNG ATCACATGGT CCNGCTNGNA NNNNNNGNNC 961 NCNGCCNGGN NTTACNNNNG GNANNNNNNA NCTGNANNAN TNNNNNNNNN N //
Primer: M13R (Pr0160) Region: insert 3'(loxP,mPGK ter,bsr 3') Sequence file: RDB13934_A6B8f.seq
>D03070A18_A6B8_1_M13R_C04_07.ab1     1 NNNNNNNNNN NNNNNNNNNN NAANGGGNNN NNAAGCTGGG TANCGGATCC TAGTGAACCT    61 CTTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAT TAAGGGTTCC   121 GGATCTCGAC CAGCTTCTGA TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG   181 TATGTGGAAC AGAGGCTGCT GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA   241 CCACAGTACT TAGAACCACA AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT   301 CTATAGATCT ATAGATCATG AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG   361 TTTAAATTAT GATATCCAAC TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG   421 TAAAGAGCAG GCATTTATCT AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC   481 ATTCAAAATG TAGGTACTCT GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC   541 CATTTTAGTG GACATCTTTA TTGTTTAATA GATCATCAAT TTCTGCATCC CGGGGATCTG   601 ATATCATCGA TGCATGGGGT CGTGCGCTCC TTTCGGTCGG GCGCTGCGGG TCGTGGGGCG   661 GGCGGCTAGC TTAATTTCGG GTATATTTGA GTGGAATGAG TTCTTCAATC GTAGTTTTGA   721 CTAACTTGCC ATTCATTTCT ATTAACACAA AACAATCTGG TGCATAGTCT GAAATCAACT   781 CCCTACACAT ACCACAAGGA CTTACCACTC GAATACTTCT ATCTACTTCG TCAGANTAAG   841 GGTGTCTAAC AGCTACNATC GTGTCNAAAT CCTTTTGTCC ATTNNNANCT GCNCTACCNA   901 TCGCAANNNG CNTNNNGCAC AAACAGTTAN NCGNNCCTAT ANNCNCNTNC NATATGTACT   961 GNCNNAAAAT GANTTNCTCN NNNTTTCNNN CNNAATTNNC CNNNTCCCNN NNNTGNATGN 1021 NNNNNNATNN NNNNAANNNN NNNNNN //
Primer: PGK_N_rev (Pr0243) Region: insert mid(loxP,SV40 pA) Sequence file: RDB13934_A6B8g.seq
>D03070A18_A6B8_1_PGK-N-rev_F06_18.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGGNANNNN NNCGAGGTCG AGNNNCCTAA GCTTGGCTGG    61 ANGTAAACTC CTCTTCAGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATATT   121 AAGGGTTATT GAATATGATC GACGCGTTAA GATACATTGA TGAGTTTGGA CAAACCACAA   181 CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTNTT GCNNNNNNTG   241 TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC   301 AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCNNGTA AAACCTCTAC NAATGTGGTA   361 TGGCTGATTA TGATNNNNTA TNNNNANTAC TTGTACAGCN NGNNNNTGCC ATGTGTNNNN   421 NCGGCGGCNG NNNNGAANNN NNNNAGGACC ATGTNATNNC GCTTCNCNNN NGNNNCTTTG   481 CTCNNCTTGN NNNNNNGGNN NNNGTAGTGG NTGTCNGGNN NNANCACNGG GCCNNNNNNN 541 NNN //
Primer: PGKpro_F (Pr0249) Region: insert mid(bsr,mPGK ter 5') Sequence file: RDB13934_A6B8h.seq
>D03070A18_A6B8_1_PGK-proF_A08_02.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNTCCNGGC CTTTCGACCT GCATCCNTCT AGATCTCGAG    61 CAGCTGAAGC TTACCGCTAG CATGAAAACA TTTAACATTT CTCAACAAGA TCTAGAATTA   121 GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG ATAATAAACA TCATGTGGGA   181 GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG TACATATTGA AGCGTATATA   241 GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA GTGCAGTTTC GAATGGACAA   301 AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT CTGACGAAGT AGATAGAAGT   361 ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA TTTCAGACTA TGCACCAGAT   421 TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA CTACGATTGA AGAACTCATT   481 CCACTCAAAT ATACCCGAAA TTAAGCTAGC CGCCCGCCCC ACGACCCGCA GCGCCCGACC   541 GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT   601 GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA   661 AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG NNTGGAGCTA CGGGGGTGGG   721 GGTGGGGTGG GATTAGATAA ATGCCTGCTC TTTACTGAAG GCTCTTTACT ATTGCTTTAT   781 GATAATGTTT CATAGTTGGA TATCATAATT TAAACNAGCA AAACCAAATT NAGGGNCAGC   841 TCATTNCCTC CCACTCATGA TCTANAGATC TATAGATCTC TCGNGGGATC ATTGTTTTTC   901 TCTTGNNNNC NNNTTTGNGG NNCTNANTAC TGNGGNTTTN CANNGNGTCA GTTTNNNANC 961 CNGNANNAAC NAGNTNANNN NNCCTNNNNN NCNNNNNNNN NN //
Primer: SV40polyA_R2 (Pr0623) Region: insert mid(mClover 3') Sequence file: RDB13934_A6B8i.seq
>13934_13934_B3C8F_SV40polyA_R2_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNGNNNNNA AANNNGNNNN NNNCNACNAT TGCATTCATT TTATGTTTCA    61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT   121 GGCTGATTAT GATCAGTTAT CTAGATTACT TGTACAGCTC GTCCATGCCA TGTGTAATCC   181 CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC   241 TCAGCTTGGA CTGATGGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA   301 TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT GCCGTCCTCA ACGTTGTGGC   361 GGATCTTGAA GTTAGCCTTG ATGCCGTTCT TCTGCTTGTC GGCCGTGATA TAGACGTTGT   421 GGCTGTTGAA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC TTGAAGTCGA   481 TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC TCGGCGCGGG   541 TCTTGTAGGT ACCGTCGTCC TTGAAAGAGA TGGTGCGCTC CTGGACGTAG CCTTCGGGCA   601 TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG GTAGCGGCTG AAGCAGGCCA   661 CGCCGTANCC GAAGGTGGTC ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC   721 AGATGAACTT CANGGTCAGC TTGCCGTTGG TGGCATCGCC CTCGCCCTCG CCGCGGANGC   781 TGAACTTGTG GCCGTTTACG TCGCCNTCCA GCTCGACCAG NANGGGCACC ACCNCGGTGA   841 ACAGCTCCTC GCCCTTGCTC ACCATGCTAG CTTTATACNT CCTNNNATCN ATTTTCCNNN   901 AGNACNGNGC TNCNNCCATT GATANCTTCA CGANNGCCGC CNCNNCCGGN NNNNNGCTTG 961 NNTTTTGNCN NNAANNNNNN NCNNNCNTNN NNNNNTGATN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl