Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK291 (mAID-mClover-Bsr)

A plasmid for construction of C-terminal mAID-mClover tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13934_A6B8p1_v2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB13934_A6B8a_2.seq
>D03070A18_A6B8_1_M13(-40)_C05_08.ab1     1 AGGCCGGGGA CGCGCGTATA CGACTCACTA TAGGGCGAAT TGGAGCTCCC CGGGATCCGG    61 TGCAGGCGCC AAGGAGAAGA GTGCTTGTCC TAAAGATCCA GCCAAACCTC CGGCCAAGGC   121 ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG AACGTGATGG TTTCCTGCCA   181 AAAATCAAGC GGTGGCCCGG AGGCGGCGGC GTTCGTGAAG GTATCAATGG ACGGAGCACC   241 GTACTTGAGG AAAATCGATT TGAGGATGTA TAAAGCTAGC ATGGTGAGCA AGGGCGAGGA   301 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA   361 GTTCAGCGTC CGCGGCGAGG GCGAGGGCGA TGCCACCAAC GGCAAGCTGA CCCTGAAGTT   421 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCTTCGGCTA   481 CGGCGTGGCC TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC   541 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTCT TTCAAGGACG ACGGTACCTA   601 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA   661 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTTCAA   721 CAGCCACAAC GTCTATATCA CGGCCGACAA GCAGAAGAAC GGCATCAAGG CTAACTTCAA   781 GATCCGCCAC AACGTTGAGG ACGGCAGCGT GCAGCTCGCC GGACCACTAC CAGCAGAACA   841 CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC CATCAGTCCA   901 AGCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGGA GTTCGTGACC   961 GCCGCCGGGG ATTACACATG GCATGGACGA GCTGTACAAG TAATCTAGAT AACTGATCAT  1021 ATCAGCCATA CCACATTTGT AGGAGGTTTT ACTGGCTTAA AAACCTCCCA CACTCCCCCT  1081 TGACCTGAAC ATTAATGATG CCATTGGATG TTGGTACTTG GTTTATTGCC AGCCTTATAT 1141 AATGTGGCT //
Primer: PGK_N_rev Sequence file: RDB13934_A6B8b_2.seq
>D03070A18_A6B8_1_PGK-N-rev_F06_18.ab1     1 CATGGGACGC CTCCCCTACC CCGGTAGAAT TTCGAGGTCG AGAATCCTAA GCTTGGCTGG    61 ACGTAAACTC CTCTTCAGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATATT   121 AAGGGTTATT GAATATGATC GACGCGTTAA GATACATTGA TGAGTTTGGA CAAACCACAA   181 CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG   241 TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC   301 AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA   361 TGGCTGATTA TGATCAGTTA TCTAGATTAC TTGTACAGCT CGTCCATGCC ATGTGTAATC   421 CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG   481 CTCAGCTTGG ACTGATGGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCG 541 ATGGGGGTGT TCTGCTGGTA GT //
Primer: PGKpro_F Sequence file: RDB13934_A6B8c_2.seq
>D03070A18_A6B8_1_PGK-proF_A08_02.ab1     1 AGTCGGCGAT CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCATCCATCT AGATCTCGAG    61 CAGCTGAAGC TTACCGCTAG CATGAAAACA TTTAACATTT CTCAACAAGA TCTAGAATTA   121 GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG ATAATAAACA TCATGTGGGA   181 GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG TACATATTGA AGCGTATATA   241 GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA GTGCAGTTTC GAATGGACAA   301 AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT CTGACGAAGT AGATAGAAGT   361 ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA TTTCAGACTA TGCACCAGAT   421 TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA CTACGATTGA AGAACTCATT   481 CCACTCAAAT ATACCCGAAA TTAAGCTAGC CGCCCGCCCC ACGACCCGCA GCGCCCGACC   541 GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT   601 GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA   661 AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG ATTGGAGCTA CGGGGGTGGG   721 GGTGGGGTGG GATTAGATAA ATGCCTGCTC TTTACTGAAG GCTCTTTACT ATTGCTTTAT   781 GATAATGTTT CATAGTTGGA TATCATAATT TAAACAAGCA AAACCAAATT AAGGGCCAGC   841 TCATTCCCTC CCACTCATGA TCTATAGATC TATAGATCTC TCGTGGGATC ATTGTTTTTC   901 TCTTGATTCC CACTTTGTGG TTCTAAGTAC TGTGGGTTTC CAATGTGTCA GTTTCATAGC   961 CTGAAAGAAC GAGATCAGCA AGCCTCTGTT CCCACATACA CTTTCATTTC TCAGTAATTG  1021 TTTGCCAAGG TTCTAAATTT CCATCCAGAG GCTGGTCGAG AATCCGGAAC CCCTTCAATA  1081 TACTTCGTAA TAATGTATGG CCTATACCGA GTATAGTCTC GAAGAAGGTT CAACTAGGAT 1141 TCCAGTTAAC CACCAGCCGT TG //
Primer: M13R Sequence file: RDB13934_A6B8d_2.seq
>D03070A18_A6B8_1_M13R_C04_07.ab1     1 CCCCGGGCAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGG TACCGGATCC TAGTGAACCT    61 CTTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAT TAAGGGTTCC   121 GGATCTCGAC CAGCTTCTGA TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG   181 TATGTGGAAC AGAGGCTGCT GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA   241 CCACAGTACT TAGAACCACA AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT   301 CTATAGATCT ATAGATCATG AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG   361 TTTAAATTAT GATATCCAAC TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG   421 TAAAGAGCAG GCATTTATCT AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC   481 ATTCAAAATG TAGGTACTCT GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC   541 CATTTTAGTG GACATCTTTA TTGTTTAATA GATCATCAAT TTCTGCATCC CGGGGATCTG   601 ATATCATCGA TGCATGGGGT CGTGCGCTCC TTTCGGTCGG GCGCTGCGGG TCGTGGGGCG   661 GGCGGCTAGC TTAATTTCGG GTATATTTGA GTGGAATGAG TTCTTCAATC GTAGTTTTGA   721 CTAACTTGCC ATTCATTTCT ATTAACACAA AACAATCTGG TGCATAGTCT GAAATCAACT   781 CCCTACACAT ACCACAAGGA CTTACCACTC GAATACTTCT ATCTACTTCG TCAGAATAAG   841 GGTGTCTAAC AGCTACAATC GTGTCAAAAT CCTTTTGTCC ATTCGAAACT GCACTACCAA   901 TCGCAAATGG CTTTCTGCAC AAACAGTTAC TCGTCCCTAT ATACGCTTTC AATATGTACT   961 GCCCGAAAAT GATTTTCTCC TGTTTTCGTA CGAAATTTGC CGCCTCCCCA CCATGAATGT  1021 TTAATTATCC TCATAAAGGC ATTGTAATTC TTTCTCTGTC GCCTACTTTC TACTAAATTT  1081 CTAGAATCTG TGGAGAAATT GTTTAAAATG GATTTTCATG CCTAGCGGTA AGCCTTCAGC 1141 CTGACTCGAA GGAATCTCTA GAAAATTGGA GTTGTGCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl