A plasmid for construction of C-terminal mAID-mClover tagging donors.
Alternative name | mAID-mClover-Bsr, 1024 |
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Clone info. | A plasmid for construction of C-terminal mAID-mClover tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone. |
Comment | To make 0.5 M stock solution, dissolve indole-3-acetic acid (IAA) (Sigma-Aldrich, 45533) or 1-naphthaleneacetic acid (NAA) (Sigma-Aldrich, 35745) in 100 % DMSO. IAA solution can be made in 100 % ethanol. Aliquots can be kept for 6 months in the dark at -20oC (Devrekanli, A., Kanemaki, M.T. Methods in Molecular Biology, 1369, 257-278, 2016). |
Vector backbone | pBluescriptII KS- (Plasmid) |
Size of vector backbone | 3.0 kb |
Selectable markers | Am^r |
Gene/insert name | Arabidopsis thaliana AtIAA17 cDNA Aequorea victoria GFP cDNA |
Depositor|Developer | Kanemaki, Masato | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() MTA, for use for for-profit purpose [Word ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 提供同意書 (MTA, 営利目的用)[Word ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13934 | pMK291 (mAID-mClover-Bsr) | DNA solution |
Materials & Methods section:
The pMK291 (mAID-mClover-Bsr) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13934). |
Reference section:
Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13934_A6B8p1-3.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: M13_-40 (Pr0410) Region: insert mid(Linker,mAID,mClover 5') Sequence file: RDB13934_A6B8e.seq ![]() |
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>D03070A18_A6B8_1_M13(-40)_C05_08.ab1 1 NNNNNNNNNN NNCNCNNANN NNNNTNNCNA TAGGGCGAAT TGGAGCTCCC CGGGNTCCGG 61 TGCAGGCGCC AAGGAGAAGA GTGCTTGTCC TAAAGATCCA GCCAAACCTC CGGCCAAGGC 121 ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG AACGTGATGG TTTCCTGCCA 181 AAAATCAAGC GGTGGCCCGG AGGCGGCGGC GTTCGTGAAG GTATCAATGG ACGGAGCACC 241 GTACTTGAGG AAAATCGATT TGAGGATGTA TAAAGCTAGC ATGGTGAGCA AGGGCGAGGA 301 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA 361 GTTCAGCGTC CGCGGCGAGG GCGAGGGCGA TGCCACCAAC GGCAAGCTGA CCCTGAAGTT 421 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCTTCGGCTA 481 CGGCGTGGCC TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC 541 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTCT TTCAAGGACG ACGGTACCTA 601 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA 661 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTTCAA 721 CAGCCACAAC GTCTATATCA CGGCCGACAA GCAGAAGANC GGCATCAAGG CTAACTTCAA 781 GATCCGCCAC AACGTTGAGG ACGGCAGCGT GCAGCTCGCC NNACCACTAC CANCNNAACA 841 CCCCCATCGG CGANNGNCCC GNNCTGCTGC CCGANANCCA CTACCTGAGC CATCAGTNCA 901 AGCTGANCNA AGANCCCNNC NANAAGCNNG ATCACATGGT CCNGCTNGNA NNNNNNGNNC 961 NCNGCCNGGN NTTACNNNNG GNANNNNNNA NCTGNANNAN TNNNNNNNNN N // |
Primer: M13R (Pr0160) Region: insert 3'(loxP,mPGK ter,bsr 3') Sequence file: RDB13934_A6B8f.seq ![]() |
>D03070A18_A6B8_1_M13R_C04_07.ab1 1 NNNNNNNNNN NNNNNNNNNN NAANGGGNNN NNAAGCTGGG TANCGGATCC TAGTGAACCT 61 CTTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAT TAAGGGTTCC 121 GGATCTCGAC CAGCTTCTGA TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG 181 TATGTGGAAC AGAGGCTGCT GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA 241 CCACAGTACT TAGAACCACA AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT 301 CTATAGATCT ATAGATCATG AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG 361 TTTAAATTAT GATATCCAAC TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG 421 TAAAGAGCAG GCATTTATCT AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC 481 ATTCAAAATG TAGGTACTCT GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC 541 CATTTTAGTG GACATCTTTA TTGTTTAATA GATCATCAAT TTCTGCATCC CGGGGATCTG 601 ATATCATCGA TGCATGGGGT CGTGCGCTCC TTTCGGTCGG GCGCTGCGGG TCGTGGGGCG 661 GGCGGCTAGC TTAATTTCGG GTATATTTGA GTGGAATGAG TTCTTCAATC GTAGTTTTGA 721 CTAACTTGCC ATTCATTTCT ATTAACACAA AACAATCTGG TGCATAGTCT GAAATCAACT 781 CCCTACACAT ACCACAAGGA CTTACCACTC GAATACTTCT ATCTACTTCG TCAGANTAAG 841 GGTGTCTAAC AGCTACNATC GTGTCNAAAT CCTTTTGTCC ATTNNNANCT GCNCTACCNA 901 TCGCAANNNG CNTNNNGCAC AAACAGTTAN NCGNNCCTAT ANNCNCNTNC NATATGTACT 961 GNCNNAAAAT GANTTNCTCN NNNTTTCNNN CNNAATTNNC CNNNTCCCNN NNNTGNATGN 1021 NNNNNNATNN NNNNAANNNN NNNNNN // |
Primer: PGK_N_rev (Pr0243) Region: insert mid(loxP,SV40 pA) Sequence file: RDB13934_A6B8g.seq ![]() |
>D03070A18_A6B8_1_PGK-N-rev_F06_18.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGGNANNNN NNCGAGGTCG AGNNNCCTAA GCTTGGCTGG 61 ANGTAAACTC CTCTTCAGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATATT 121 AAGGGTTATT GAATATGATC GACGCGTTAA GATACATTGA TGAGTTTGGA CAAACCACAA 181 CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTNTT GCNNNNNNTG 241 TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC 301 AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCNNGTA AAACCTCTAC NAATGTGGTA 361 TGGCTGATTA TGATNNNNTA TNNNNANTAC TTGTACAGCN NGNNNNTGCC ATGTGTNNNN 421 NCGGCGGCNG NNNNGAANNN NNNNAGGACC ATGTNATNNC GCTTCNCNNN NGNNNCTTTG 481 CTCNNCTTGN NNNNNNGGNN NNNGTAGTGG NTGTCNGGNN NNANCACNGG GCCNNNNNNN 541 NNN // |
Primer: PGKpro_F (Pr0249) Region: insert mid(bsr,mPGK ter 5') Sequence file: RDB13934_A6B8h.seq ![]() |
>D03070A18_A6B8_1_PGK-proF_A08_02.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNTCCNGGC CTTTCGACCT GCATCCNTCT AGATCTCGAG 61 CAGCTGAAGC TTACCGCTAG CATGAAAACA TTTAACATTT CTCAACAAGA TCTAGAATTA 121 GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG ATAATAAACA TCATGTGGGA 181 GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG TACATATTGA AGCGTATATA 241 GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA GTGCAGTTTC GAATGGACAA 301 AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT CTGACGAAGT AGATAGAAGT 361 ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA TTTCAGACTA TGCACCAGAT 421 TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA CTACGATTGA AGAACTCATT 481 CCACTCAAAT ATACCCGAAA TTAAGCTAGC CGCCCGCCCC ACGACCCGCA GCGCCCGACC 541 GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT 601 GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA 661 AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG NNTGGAGCTA CGGGGGTGGG 721 GGTGGGGTGG GATTAGATAA ATGCCTGCTC TTTACTGAAG GCTCTTTACT ATTGCTTTAT 781 GATAATGTTT CATAGTTGGA TATCATAATT TAAACNAGCA AAACCAAATT NAGGGNCAGC 841 TCATTNCCTC CCACTCATGA TCTANAGATC TATAGATCTC TCGNGGGATC ATTGTTTTTC 901 TCTTGNNNNC NNNTTTGNGG NNCTNANTAC TGNGGNTTTN CANNGNGTCA GTTTNNNANC 961 CNGNANNAAC NAGNTNANNN NNCCTNNNNN NCNNNNNNNN NN // |
Primer: SV40polyA_R2 (Pr0623) Region: insert mid(mClover 3') Sequence file: RDB13934_A6B8i.seq ![]() |
>13934_13934_B3C8F_SV40polyA_R2_D01_04_ABI08.ab1 1 NNNNNNNNNN NNNGNNNNNA AANNNGNNNN NNNCNACNAT TGCATTCATT TTATGTTTCA 61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT 121 GGCTGATTAT GATCAGTTAT CTAGATTACT TGTACAGCTC GTCCATGCCA TGTGTAATCC 181 CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC 241 TCAGCTTGGA CTGATGGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA 301 TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT GCCGTCCTCA ACGTTGTGGC 361 GGATCTTGAA GTTAGCCTTG ATGCCGTTCT TCTGCTTGTC GGCCGTGATA TAGACGTTGT 421 GGCTGTTGAA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC TTGAAGTCGA 481 TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC TCGGCGCGGG 541 TCTTGTAGGT ACCGTCGTCC TTGAAAGAGA TGGTGCGCTC CTGGACGTAG CCTTCGGGCA 601 TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG GTAGCGGCTG AAGCAGGCCA 661 CGCCGTANCC GAAGGTGGTC ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC 721 AGATGAACTT CANGGTCAGC TTGCCGTTGG TGGCATCGCC CTCGCCCTCG CCGCGGANGC 781 TGAACTTGTG GCCGTTTACG TCGCCNTCCA GCTCGACCAG NANGGGCACC ACCNCGGTGA 841 ACAGCTCCTC GCCCTTGCTC ACCATGCTAG CTTTATACNT CCTNNNATCN ATTTTCCNNN 901 AGNACNGNGC TNCNNCCATT GATANCTTCA CGANNGCCGC CNCNNCCGGN NNNNNGCTTG 961 NNTTTTGNCN NNAANNNNNN NCNNNCNTNN NNNNNTGATN NNNNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP] |
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reference | Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318. |
reference | Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302. |
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