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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK291 (mAID-mClover-Bsr)

A plasmid for construction of C-terminal mAID-mClover tagging donors.

Catalog number RDB13934
Resource name pMK291 (mAID-mClover-Bsr)
Alternative name mAID-mClover-Bsr, 1024
Clone info. A plasmid for construction of C-terminal mAID-mClover tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
Comment To make 0.5 M stock solution, dissolve indole-3-acetic acid (IAA) (Sigma-Aldrich, 45533) or 1-naphthaleneacetic acid (NAA) (Sigma-Aldrich, 35745) in 100 % DMSO. IAA solution can be made in 100 % ethanol. Aliquots can be kept for 6 months in the dark at -20oC (Devrekanli, A., Kanemaki, M.T. Methods in Molecular Biology, 1369, 257-278, 2016).
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Arabidopsis thaliana AtIAA17 Aequorea victoria GFP

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
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          手続きの概要は、「提供申込みについて[link]」をご覧ください。

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          RDB13934 pMK291 (mAID-mClover-Bsr) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pMK291 (mAID-mClover-Bsr) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13934).

          Reference section:

          Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13934_A6B8p1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40 (Pr0410)
          Region: insert mid(Linker,mAID,mClover 5')
          Sequence file: RDB13934_A6B8e.seq check
          >D03070A18_A6B8_1_M13(-40)_C05_08.ab1
              1 NNNNNNNNNN NNCNCNNANN NNNNTNNCNA TAGGGCGAAT TGGAGCTCCC CGGGNTCCGG
             61 TGCAGGCGCC AAGGAGAAGA GTGCTTGTCC TAAAGATCCA GCCAAACCTC CGGCCAAGGC
            121 ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG AACGTGATGG TTTCCTGCCA
            181 AAAATCAAGC GGTGGCCCGG AGGCGGCGGC GTTCGTGAAG GTATCAATGG ACGGAGCACC
            241 GTACTTGAGG AAAATCGATT TGAGGATGTA TAAAGCTAGC ATGGTGAGCA AGGGCGAGGA
            301 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA
            361 GTTCAGCGTC CGCGGCGAGG GCGAGGGCGA TGCCACCAAC GGCAAGCTGA CCCTGAAGTT
            421 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCTTCGGCTA
            481 CGGCGTGGCC TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC
            541 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTCT TTCAAGGACG ACGGTACCTA
            601 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA
            661 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTTCAA
            721 CAGCCACAAC GTCTATATCA CGGCCGACAA GCAGAAGANC GGCATCAAGG CTAACTTCAA
            781 GATCCGCCAC AACGTTGAGG ACGGCAGCGT GCAGCTCGCC NNACCACTAC CANCNNAACA
            841 CCCCCATCGG CGANNGNCCC GNNCTGCTGC CCGANANCCA CTACCTGAGC CATCAGTNCA
            901 AGCTGANCNA AGANCCCNNC NANAAGCNNG ATCACATGGT CCNGCTNGNA NNNNNNGNNC
          961 NCNGCCNGGN NTTACNNNNG GNANNNNNNA NCTGNANNAN TNNNNNNNNN N
          //
          Primer: M13R (Pr0160)
          Region: insert 3'(loxP,mPGK ter,bsr 3')
          Sequence file: RDB13934_A6B8f.seq check
          >D03070A18_A6B8_1_M13R_C04_07.ab1
              1 NNNNNNNNNN NNNNNNNNNN NAANGGGNNN NNAAGCTGGG TANCGGATCC TAGTGAACCT
             61 CTTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAT TAAGGGTTCC
            121 GGATCTCGAC CAGCTTCTGA TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG
            181 TATGTGGAAC AGAGGCTGCT GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA
            241 CCACAGTACT TAGAACCACA AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT
            301 CTATAGATCT ATAGATCATG AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG
            361 TTTAAATTAT GATATCCAAC TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG
            421 TAAAGAGCAG GCATTTATCT AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC
            481 ATTCAAAATG TAGGTACTCT GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC
            541 CATTTTAGTG GACATCTTTA TTGTTTAATA GATCATCAAT TTCTGCATCC CGGGGATCTG
            601 ATATCATCGA TGCATGGGGT CGTGCGCTCC TTTCGGTCGG GCGCTGCGGG TCGTGGGGCG
            661 GGCGGCTAGC TTAATTTCGG GTATATTTGA GTGGAATGAG TTCTTCAATC GTAGTTTTGA
            721 CTAACTTGCC ATTCATTTCT ATTAACACAA AACAATCTGG TGCATAGTCT GAAATCAACT
            781 CCCTACACAT ACCACAAGGA CTTACCACTC GAATACTTCT ATCTACTTCG TCAGANTAAG
            841 GGTGTCTAAC AGCTACNATC GTGTCNAAAT CCTTTTGTCC ATTNNNANCT GCNCTACCNA
            901 TCGCAANNNG CNTNNNGCAC AAACAGTTAN NCGNNCCTAT ANNCNCNTNC NATATGTACT
            961 GNCNNAAAAT GANTTNCTCN NNNTTTCNNN CNNAATTNNC CNNNTCCCNN NNNTGNATGN
          1021 NNNNNNATNN NNNNAANNNN NNNNNN
          //
          Primer: PGK_N_rev (Pr0243)
          Region: insert mid(loxP,SV40 pA)
          Sequence file: RDB13934_A6B8g.seq check
          >D03070A18_A6B8_1_PGK-N-rev_F06_18.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGGNANNNN NNCGAGGTCG AGNNNCCTAA GCTTGGCTGG
             61 ANGTAAACTC CTCTTCAGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATATT
            121 AAGGGTTATT GAATATGATC GACGCGTTAA GATACATTGA TGAGTTTGGA CAAACCACAA
            181 CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTNTT GCNNNNNNTG
            241 TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC
            301 AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCNNGTA AAACCTCTAC NAATGTGGTA
            361 TGGCTGATTA TGATNNNNTA TNNNNANTAC TTGTACAGCN NGNNNNTGCC ATGTGTNNNN
            421 NCGGCGGCNG NNNNGAANNN NNNNAGGACC ATGTNATNNC GCTTCNCNNN NGNNNCTTTG
            481 CTCNNCTTGN NNNNNNGGNN NNNGTAGTGG NTGTCNGGNN NNANCACNGG GCCNNNNNNN
          541 NNN
          //
          Primer: PGKpro_F (Pr0249)
          Region: insert mid(bsr,mPGK ter 5')
          Sequence file: RDB13934_A6B8h.seq check
          >D03070A18_A6B8_1_PGK-proF_A08_02.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNTCCNGGC CTTTCGACCT GCATCCNTCT AGATCTCGAG
             61 CAGCTGAAGC TTACCGCTAG CATGAAAACA TTTAACATTT CTCAACAAGA TCTAGAATTA
            121 GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG ATAATAAACA TCATGTGGGA
            181 GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG TACATATTGA AGCGTATATA
            241 GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA GTGCAGTTTC GAATGGACAA
            301 AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT CTGACGAAGT AGATAGAAGT
            361 ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA TTTCAGACTA TGCACCAGAT
            421 TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA CTACGATTGA AGAACTCATT
            481 CCACTCAAAT ATACCCGAAA TTAAGCTAGC CGCCCGCCCC ACGACCCGCA GCGCCCGACC
            541 GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT
            601 GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA
            661 AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG NNTGGAGCTA CGGGGGTGGG
            721 GGTGGGGTGG GATTAGATAA ATGCCTGCTC TTTACTGAAG GCTCTTTACT ATTGCTTTAT
            781 GATAATGTTT CATAGTTGGA TATCATAATT TAAACNAGCA AAACCAAATT NAGGGNCAGC
            841 TCATTNCCTC CCACTCATGA TCTANAGATC TATAGATCTC TCGNGGGATC ATTGTTTTTC
            901 TCTTGNNNNC NNNTTTGNGG NNCTNANTAC TGNGGNTTTN CANNGNGTCA GTTTNNNANC
          961 CNGNANNAAC NAGNTNANNN NNCCTNNNNN NCNNNNNNNN NN
          //
          Primer: SV40polyA_R2 (Pr0623)
          Region: insert mid(mClover 3')
          Sequence file: RDB13934_A6B8i.seq check
          >13934_13934_B3C8F_SV40polyA_R2_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNGNNNNNA AANNNGNNNN NNNCNACNAT TGCATTCATT TTATGTTTCA
             61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT
            121 GGCTGATTAT GATCAGTTAT CTAGATTACT TGTACAGCTC GTCCATGCCA TGTGTAATCC
            181 CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC
            241 TCAGCTTGGA CTGATGGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA
            301 TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT GCCGTCCTCA ACGTTGTGGC
            361 GGATCTTGAA GTTAGCCTTG ATGCCGTTCT TCTGCTTGTC GGCCGTGATA TAGACGTTGT
            421 GGCTGTTGAA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC TTGAAGTCGA
            481 TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC TCGGCGCGGG
            541 TCTTGTAGGT ACCGTCGTCC TTGAAAGAGA TGGTGCGCTC CTGGACGTAG CCTTCGGGCA
            601 TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG GTAGCGGCTG AAGCAGGCCA
            661 CGCCGTANCC GAAGGTGGTC ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC
            721 AGATGAACTT CANGGTCAGC TTGCCGTTGG TGGCATCGCC CTCGCCCTCG CCGCGGANGC
            781 TGAACTTGTG GCCGTTTACG TCGCCNTCCA GCTCGACCAG NANGGGCACC ACCNCGGTGA
            841 ACAGCTCCTC GCCCTTGCTC ACCATGCTAG CTTTATACNT CCTNNNATCN ATTTTCCNNN
            901 AGNACNGNGC TNCNNCCATT GATANCTTCA CGANNGCCGC CNCNNCCGGN NNNNNGCTTG
          961 NNTTTTGNCN NNAANNNNNN NCNNNCNTNN NNNNNTGATN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP]
          reference Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318.
          reference Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302.

          2023.10.05

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