Resource data sheet
Prev.

pMK291 (mAID-mClover-Bsr)

A plasmid for construction of C-terminal mAID-mClover tagging donors.

Catalog number RDB13934
Resource name pMK291 (mAID-mClover-Bsr)
Alternative name mAID-mClover-Bsr, 1024
Clone info. A plasmid for construction of C-terminal mAID-mClover tagging donors. Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use this clone.
To make 0.5 M stock solution, dissolve indole-3-acetic acid (IAA) (Sigma-Aldrich, 45533) or 1-naphthaleneacetic acid (NAA) (Sigma-Aldrich, 35745) in 100 % DMSO. IAA solution can be made in 100 % ethanol. Aliquots can be kept for 6 months in the dark at -20oC (Devrekanli, A., Kanemaki, M.T. Methods in Molecular Biology, 1369, 257-278, 2016).
Vector backbone pBluescriptII KS- (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Am^r
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Aequorea victoria GFP cDNA
Depositor Kanemaki, Masato |
 
Other clones in our bank Arabidopsis thaliana AtIAA17 (KEGG orthology K14484) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13934 pMK291 (mAID-mClover-Bsr) DNA solution

cDNA Request [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Auxin Inducible Degron (AID) System (English text)
Featured content Auxin Inducible Degron (AID) System (Japanese text)

References

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13934_A6B8p1_v2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB13934_A6B8a_2.seq
>D03070A18_A6B8_1_M13(-40)_C05_08.ab1
    1 AGGCCGGGGA CGCGCGTATA CGACTCACTA TAGGGCGAAT TGGAGCTCCC CGGGATCCGG
   61 TGCAGGCGCC AAGGAGAAGA GTGCTTGTCC TAAAGATCCA GCCAAACCTC CGGCCAAGGC
  121 ACAAGTTGTG GGATGGCCAC CGGTGAGATC ATACCGGAAG AACGTGATGG TTTCCTGCCA
  181 AAAATCAAGC GGTGGCCCGG AGGCGGCGGC GTTCGTGAAG GTATCAATGG ACGGAGCACC
  241 GTACTTGAGG AAAATCGATT TGAGGATGTA TAAAGCTAGC ATGGTGAGCA AGGGCGAGGA
  301 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA
  361 GTTCAGCGTC CGCGGCGAGG GCGAGGGCGA TGCCACCAAC GGCAAGCTGA CCCTGAAGTT
  421 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCTTCGGCTA
  481 CGGCGTGGCC TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC
  541 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTCT TTCAAGGACG ACGGTACCTA
  601 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA
  661 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTTCAA
  721 CAGCCACAAC GTCTATATCA CGGCCGACAA GCAGAAGAAC GGCATCAAGG CTAACTTCAA
  781 GATCCGCCAC AACGTTGAGG ACGGCAGCGT GCAGCTCGCC GGACCACTAC CAGCAGAACA
  841 CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC CATCAGTCCA
  901 AGCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGGA GTTCGTGACC
  961 GCCGCCGGGG ATTACACATG GCATGGACGA GCTGTACAAG TAATCTAGAT AACTGATCAT
 1021 ATCAGCCATA CCACATTTGT AGGAGGTTTT ACTGGCTTAA AAACCTCCCA CACTCCCCCT
 1081 TGACCTGAAC ATTAATGATG CCATTGGATG TTGGTACTTG GTTTATTGCC AGCCTTATAT
1141 AATGTGGCT
//
Primer: PGK_N_rev RDB13934_A6B8b_2.seq
>D03070A18_A6B8_1_PGK-N-rev_F06_18.ab1
    1 CATGGGACGC CTCCCCTACC CCGGTAGAAT TTCGAGGTCG AGAATCCTAA GCTTGGCTGG
   61 ACGTAAACTC CTCTTCAGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATATT
  121 AAGGGTTATT GAATATGATC GACGCGTTAA GATACATTGA TGAGTTTGGA CAAACCACAA
  181 CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG
  241 TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC
  301 AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA
  361 TGGCTGATTA TGATCAGTTA TCTAGATTAC TTGTACAGCT CGTCCATGCC ATGTGTAATC
  421 CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG
  481 CTCAGCTTGG ACTGATGGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCG
541 ATGGGGGTGT TCTGCTGGTA GT
//
Primer: PGKpro_F RDB13934_A6B8c_2.seq
>D03070A18_A6B8_1_PGK-proF_A08_02.ab1
    1 AGTCGGCGAT CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCATCCATCT AGATCTCGAG
   61 CAGCTGAAGC TTACCGCTAG CATGAAAACA TTTAACATTT CTCAACAAGA TCTAGAATTA
  121 GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG ATAATAAACA TCATGTGGGA
  181 GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG TACATATTGA AGCGTATATA
  241 GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA GTGCAGTTTC GAATGGACAA
  301 AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT CTGACGAAGT AGATAGAAGT
  361 ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA TTTCAGACTA TGCACCAGAT
  421 TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA CTACGATTGA AGAACTCATT
  481 CCACTCAAAT ATACCCGAAA TTAAGCTAGC CGCCCGCCCC ACGACCCGCA GCGCCCGACC
  541 GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT
  601 GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA
  661 AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG ATTGGAGCTA CGGGGGTGGG
  721 GGTGGGGTGG GATTAGATAA ATGCCTGCTC TTTACTGAAG GCTCTTTACT ATTGCTTTAT
  781 GATAATGTTT CATAGTTGGA TATCATAATT TAAACAAGCA AAACCAAATT AAGGGCCAGC
  841 TCATTCCCTC CCACTCATGA TCTATAGATC TATAGATCTC TCGTGGGATC ATTGTTTTTC
  901 TCTTGATTCC CACTTTGTGG TTCTAAGTAC TGTGGGTTTC CAATGTGTCA GTTTCATAGC
  961 CTGAAAGAAC GAGATCAGCA AGCCTCTGTT CCCACATACA CTTTCATTTC TCAGTAATTG
 1021 TTTGCCAAGG TTCTAAATTT CCATCCAGAG GCTGGTCGAG AATCCGGAAC CCCTTCAATA
 1081 TACTTCGTAA TAATGTATGG CCTATACCGA GTATAGTCTC GAAGAAGGTT CAACTAGGAT
1141 TCCAGTTAAC CACCAGCCGT TG
//
Primer: M13R RDB13934_A6B8d_2.seq
>D03070A18_A6B8_1_M13R_C04_07.ab1
    1 CCCCGGGCAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGG TACCGGATCC TAGTGAACCT
   61 CTTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAT TAAGGGTTCC
  121 GGATCTCGAC CAGCTTCTGA TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG
  181 TATGTGGAAC AGAGGCTGCT GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA
  241 CCACAGTACT TAGAACCACA AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT
  301 CTATAGATCT ATAGATCATG AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG
  361 TTTAAATTAT GATATCCAAC TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG
  421 TAAAGAGCAG GCATTTATCT AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC
  481 ATTCAAAATG TAGGTACTCT GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC
  541 CATTTTAGTG GACATCTTTA TTGTTTAATA GATCATCAAT TTCTGCATCC CGGGGATCTG
  601 ATATCATCGA TGCATGGGGT CGTGCGCTCC TTTCGGTCGG GCGCTGCGGG TCGTGGGGCG
  661 GGCGGCTAGC TTAATTTCGG GTATATTTGA GTGGAATGAG TTCTTCAATC GTAGTTTTGA
  721 CTAACTTGCC ATTCATTTCT ATTAACACAA AACAATCTGG TGCATAGTCT GAAATCAACT
  781 CCCTACACAT ACCACAAGGA CTTACCACTC GAATACTTCT ATCTACTTCG TCAGAATAAG
  841 GGTGTCTAAC AGCTACAATC GTGTCAAAAT CCTTTTGTCC ATTCGAAACT GCACTACCAA
  901 TCGCAAATGG CTTTCTGCAC AAACAGTTAC TCGTCCCTAT ATACGCTTTC AATATGTACT
  961 GCCCGAAAAT GATTTTCTCC TGTTTTCGTA CGAAATTTGC CGCCTCCCCA CCATGAATGT
 1021 TTAATTATCC TCATAAAGGC ATTGTAATTC TTTCTCTGTC GCCTACTTTC TACTAAATTT
 1081 CTAGAATCTG TGGAGAAATT GTTTAAAATG GATTTTCATG CCTAGCGGTA AGCCTTCAGC
1141 CTGACTCGAA GGAATCTCTA GAAAATTGGA GTTGTGCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166.
reference Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318.
reference Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302 .

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl